Guida Maria Portela‐Gomes, Mats Stridsberg,
... Immunoreactivity in the Human Pancreatic Islet Cell Types GUIDA MARIA PORTELA-GOMES, Corresponding Author GUIDA MARIA PORTELA-GOMES Centre of Gastroenterology and of Nutrition, ... of Pathology, Uppsala University, Uppsala, SwedenAddress for correspondence: Guida Maria Portela-Gomes, Department of Genetics and Pathology, Unit ... Uppsala, SwedenSearch for more papers by this author GUIDA MARIA PORTELA-GOMES, Corresponding Author GUIDA MARIA PORTELA-GOMES Centre of Gastroenterology and of Nutrition, ... of Pathology, Uppsala University, Uppsala, SwedenAddress for correspondence: Guida Maria Portela-Gomes, Department of Genetics and Pathology, Unit ...
Tópico(s): Cellular transport and secretion
2002 - Wiley | Annals of the New York Academy of Sciences
Tobias Hermle, María Clara Guida, Samuel J. Beck, Susanne Helmstädter, Matias Simons,
... for more papers by this author Maria Clara Guida Maria Clara Guida Center for Systems Biology (ZBSA), University ... for more papers by this author Maria Clara Guida Maria Clara Guida Center for Systems Biology (ZBSA), University of Freiburg, ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2013 - Springer Nature | The EMBO Journal
Erik Wilander, Guida Maria Portela‐Gomes, Lars Grimelius, Per Westermark,
Tópico(s): Neuropeptides and Animal Physiology
1977 - Elsevier BV | Gastroenterology
Erik Wilander, Guida Maria Portela‐Gomes, Lars Grimelius, G. Lundqvist, Valdemar Skoog,
A specific immunofluorescence for enteroglucagon or substance P or for both hormones was demonstrated in nine out of 12 examined rectal carcinoids. One tumor was argentaffin, contained ultrastructurally pleomorphic granules of the entero-chromaffin cell type, and showed immunofluorescence for substance P. The rest were non-argentaffin but were argyrophil with the Grimelius technique and contained round granules. The argyrophil carcinoids were immunoreactive to one or both hormones in eight cases ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
1977 - Springer Science+Business Media | Virchows Archiv B Cell Pathology
Guida Maria Portela‐Gomes, Lars Grimelius, Roland Petersson, Reinhold Bergström,
A quantitative study was made of the enterochromaffin (EC) cells, visualized with the Masson (slightly modified) and Falck-Hillarp techniques, in the rat gastrointestinal tract. Different factors influencing the quantification were analysed and the magnitude of methodological errors was estimated. The two histochemical techniques visualized the EC cells in different ways, influencing the quantification results. This means that a comparison of the quantitative results obtained by these techniques can ...
Tópico(s): Drug Solubulity and Delivery Systems
1984 - Taylor & Francis | Upsala Journal of Medical Sciences
Guida Maria Portela‐Gomes, Erik Wilander, Lars Grimelius, R. Bergström, G. Ruhn,
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
1990 - Elsevier BV | Pathology - Research and Practice
Guida Maria Portela‐Gomes, Lars Grimelius,
The staining characteristics of the ent.eroohromaffin cell system in the hU111an and rat gastrointestinal tract were studied with 3 histochemical procedures - the Masson method, formaldehydeinduced fluorescence (FIF), and immunocytochemical techniques using both monoclonal and polyclonal serotonin antisera. A simple modification of the Masson technique is described and COInpared with several other modifications of the same technique. The specificities of the 2 antisera were examined with regard to ...
Tópico(s): Gastrointestinal disorders and treatments
1986 - Elsevier BV | Acta Histochemica
Guida Maria Portela‐Gomes, Mats Stridsberg,
We studied the immunoreactivity of 12 different region-specific antibodies to the chromogranin A (CgA) molecule in the various neuroendocrine cell types of the human gastrointestinal (GI) tract by using double immunofluorescence techniques. These staining results were compared with others obtained with a commercial monoclonal CgA antibody (LK2H10). G (gastrin)-cells showed immunoreactivity to virtually all region-specific antibodies, but with varying frequency. Most intestinal EC (enterochromaffin)- ...
Tópico(s): Lipid Membrane Structure and Behavior
2002 - SAGE Publishing | Journal of Histochemistry & Cytochemistry
Guida Maria Portela–GomesM, Mats Stridsberg,
Chromogranin (Cg) B is an acidic glycoprotein present in neuroendocrine tissue. The sequence shows several dibasic amino acid positions susceptible to proteolytic cleavage. The purpose of this study was to elucidate the expression of CgB epitopes in the human endocrine pancreas. Tissue sections of six human pancreata were immunostained with 16 different region-specific antibodies to the CgB molecule, using double immunofluorescence techniques. The CgB epitope pattern varied in the four major islet ...
Tópico(s): Neuropeptides and Animal Physiology
2002 - SAGE Publishing | Journal of Histochemistry & Cytochemistry
Guida Maria Portela‐Gomes, Samy M. Abdel‐Halim,
Introduction Knowledge about the relation between G proteins and adenylyl cyclases (ACs) is important for the construction of signaling paradigms to increase our understanding of signal transduction in the normal state and its alterations in pathologic states, such as type-2 diabetes. Aims and Methodology The immunocytochemical expression patterns of the stimulatory Gs proteins (G α-s and G α-olf) and the in vitro Ca2+-stimulated ACs (AC1, 3, and 8) were studied in normal and spontaneously diabetic ...
Tópico(s): Diabetes and associated disorders
2002 - Lippincott Williams & Wilkins | Pancreas
Guida Maria Portela‐Gomes, Agneta Lukinius, Otto Ljungberg, Suad Efendić, Bo Åhrén, Samy M. Abdel‐Halim,
Tópico(s): Diabetes Treatment and Management
2003 - Elsevier BV | Regulatory Peptides
Filip Sköldberg, Guida Maria Portela‐Gomes, Lars Grimelius, Gunnar Nilsson, Jaakko Perheentupa, Corrado Betterle, Eystein S. Husebye, Jan Gustafsson, Anders Rönnblom, Fredrik Rorsman, Olle Kämpe,
Patients with autoimmune polyendocrine syndrome type 1 often have autoantibodies against neurotransmitter synthesizing enzymes, including the pyridoxal phosphate-dependent enzymes glutamic acid decarboxylase and aromatic L-amino acid decarboxylase. Using a candidate approach, we have identified the histamine-synthesizing enzyme histidine decarboxylase, also pyridoxal phosphate dependent, as an autoantigen in this disorder. Anti-histidine decarboxylase antibodies reacting with in vitro translated ...
Tópico(s): Hormonal Regulation and Hypertension
2003 - Oxford University Press | The Journal of Clinical Endocrinology & Metabolism
Mats Stridsberg, Lars Grimelius, Guida Maria Portela‐Gomes,
Chromogranins and secretogranins belong to the granin family of proteins, which are expressed in neuroendocrine and nervous tissue. In earlier publications we have described the development of region-specific antibodies against CgA and CgB. In this study we describe antibodies to SgII and SgIII and their usefulness for immunohistochemical staining. Peptides homologous to defined parts of secretogranins II and III were selected and synthesized. Antibodies were raised and immunostainings were performed ...
Tópico(s): Adrenal and Paraganglionic Tumors
2008 - Wiley | Journal of Anatomy
Apostolos V. Tsolakis, Mats Stridsberg, Lars Grimelius, Guida Maria Portela‐Gomes, Sture Falkmer, Helge L. Waldum, Eva Tiensuu Janson,
Goals Our aim was to elucidate the incidence and distribution pattern of ghrelin-immunoreactive (IR) cells in various types of human gastric endocrine tumors, and their surrounding mucosa, and relate the findings to total ghrelin concentrations in plasma. Background It has been demonstrated previously, that ghrelin-IR cells are present not only in normal human gastric oxyntic mucosa, but also in all types of enterochromaffinlike (ECL) cell carcinoids (ECL-CCs), and in mucosal regions affected by ECL ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2008 - Lippincott Williams & Wilkins | Journal of Clinical Gastroenterology
Brita Ardesjö, Guida Maria Portela‐Gomes, Fredrik Rorsman, Eva Gerdin, Lars Lööf, Lars Grimelius, Olle Kämpe, Olov Ekwall,
A number of autoantibodies have been reported in inflammatory bowel disease (IBD). The aim of this study was to investigate to what extent sera from patients with IBD contain autoantibodies directed against normal human gastrointestinal mucosa.Samples of sera from 50 patients with IBD and 50 healthy subjects were used for immunostaining of normal and affected human gastrointestinal tissues.Eighty-four percent of the sera from IBD patients showed immunoreactivity against goblet cells in the appendix ...
Tópico(s): Eosinophilic Esophagitis
2008 - Oxford University Press | Inflammatory Bowel Diseases
Kenbugul Jatta, Gabriella Eliason, Guida Maria Portela‐Gomes, Lars Grimelius, Oscar Caro, L Nilholm, Allan Sirjsö, Karin Piehl‐Aulin, Samy M. Abdel‐Halim,
Background: A significant number of patients with chronic obstructive pulmonary disease (COPD) exhibit skeletal muscle wasting and decreased capillary area formation, which correlate with increased mortality. Aim: To determine the molecular mechanisms mediating decreased capillary formation in COPD. Methods: 24 patients with COPD and 12 matching controls were recruited. Patients with COPD were classified into mild, moderate and severe groups according to GOLD (global initiative for chronic obstructive ...
Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research
2008 - BMJ | Journal of Clinical Pathology
Guida Maria Portela‐Gomes, Jiaur R. Gayen, Lars Grimelius, Mats Stridsberg, Sushil K. Mahata,
Chromogranin (Cg) A is expressed in neuroendocrine and neuronal tissues. It is involved in the generation of secretory granules and is cleaved to form biologically active peptides. Targeted ablation of the Chga gene resulted in increased plasma catecholamines, high blood pressure, and decreased size and number of adrenal medullary chromaffin granules. The aim of this study was to determine whether Chga null mice display changes in the morphology and function of the endocrine pancreas. Sections of ...
Tópico(s): Pancreatic function and diabetes
2008 - Elsevier BV | Regulatory Peptides
Guida Maria Portela‐Gomes, Lars Grimelius, Per Westermark, Mats Stridsberg,
Objectives: Somatostatin inhibits hormone release through 5 G protein-coupled somatostatin receptors (sst1-sst5). However, the role of somatostatin in islet physiology is not fully known. The immunoreactivity to sst1 to sst5 in normal human endocrine pancreas has been described. The present study reports the expression of sst1 to sst5 in human pancreatic islets with type 2 diabetes mellitus. Methods: Pancreatic autopsy specimens from individuals with type 2 diabetes mellitus and matched controls ...
Tópico(s): Metabolism, Diabetes, and Cancer
2010 - Lippincott Williams & Wilkins | Pancreas
Guida Maria Portela‐Gomes, Lars Grimelius, Mats Stridsberg,
Different epitopes of the granin family of proteins, chromogranin (Cg) A, CgB and secretogranin (Sg) II, have been demonstrated in normal human pancreas, gastrointestinal tract, adrenal medulla and in several neuroendocrine tumours (NETs). SgIII has been recently reported in endocrine pancreas. The aim of the present study was to examine the expression of SgIII in different NETs and compare it with the expression of CgA, CgB and SgII epitopes. Tissue specimens from 47 NETs were analyzed. Antibodies ...
Tópico(s): Cellular transport and secretion
2010 - Elsevier BV | Regulatory Peptides
Guida Maria Portela‐Gomes, Lars Grimelius, Erik Wilander, Mats Stridsberg,
This review focus on neuroendocrine tumours (NETs), with special reference to the immunohistochemical analysis of granins and granin-related peptides and their usefulness in identifying and characterizing the great diversity of NET types. Granins, their derived peptides, and complex protein-processing enzyme systems that cleave granins and prohormones, have to some extent cell-specific expression patterns in normal and neoplastic NE cells. The marker most commonly used in routine histopathology to ...
Tópico(s): Caveolin-1 and cellular processes
2010 - Elsevier BV | Regulatory Peptides
Guida Maria Portela‐Gomes, Lars Grimelius, Mats Stridsberg,
Prohormone convertases (PCs) are proteinases that cleave inactive prohormones to biologically active peptides. Seven PCs have been identified; two of them, PC1/3 and PC2, have only been localized in neuroendocrine (NE) tissues; a third, furin, in both endocrine and exocrine tissues. We have studied the immunoreactivity of PC1/3, PC2 and furin in the four major NE cell types of the human pancreas by using double immunofluorescence techniques. The study also included the expression of NE secretory protein ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2007 - Elsevier BV | Regulatory Peptides
Guida Maria Portela‐Gomes, Mats Stridsberg, Lars Grimelius, O.P. Rorstad, Eva Tiensuu Janson,
Tópico(s): Neuroblastoma Research and Treatments
2007 - Springer Science+Business Media | Endocrine Pathology
Guida Maria Portela‐Gomes, Mats Stridsberg, Lars Grimelius, Sture Falkmer, Sture Falkmer,
In a recent immunohistochemical study of pheochromocytomas, a difference was observed between benign and malignant pheochromocytomas in their expression in different parts of the chromogranin (Cg) A molecule. The purpose of the present study was to extend the investigations by including two other members of this Cg family, CgB and C. Twenty‐five patients operated on for clinicopathologically benign pheochromocytomas, and four for metastasizing pheochromocytomas, were studied. Expression of the different ...
Tópico(s): Pituitary Gland Disorders and Treatments
2004 - Wiley | Apmis
Mats Stridsberg, Eva Tiensuu Janson, Guida Maria Portela‐Gomes,
Cellular localisation of chromogranins and processed products in the diffuse neuroendocrine system and related tumours
Tópico(s): Retinoids in leukemia and cellular processes
2004 - Bentham Science Publishers | Current Medicinal Chemistry
Guida Maria Portela‐Gomes, Gerhard W. Hacker, Raimund Weitgasser,
The authors review the application of a variety of neuroendocrine cell markers to identify pancreatic islet cells and tumors. In the past, several empiric histochemical techniques had been used to demonstrate neuroendocrine cells, particularly the Grimelius argyrophilic stain. The development of immunohistochemistry made it possible to demonstrate specific cell products such as regulatory peptides, thus allowing the classification of pancreatic neuroendocrine tumors with a view to clinical symptoms. ...
Tópico(s): Neuroblastoma Research and Treatments
2004 - Lippincott Williams & Wilkins | Applied immunohistochemistry & molecular morphology
Apostolos V. Tsolakis, Guida Maria Portela‐Gomes, Mats Stridsberg, Lars Grimelius, Anders Sundin, Barbro Eriksson, Kjell E. Öberg, Eva Tiensuu Janson,
A characteristic feature of neuroendocrine tumors is production and release of peptide hormone. Ghrelin is a 28-amino acid hormone that stimulates GH release. In this paper, we describe a patient with a metastasizing gastric neuroendocrine tumor displaying intense immunoreactivity for ghrelin and extremely high circulating levels of ghrelin. Tumor tissue biopsies from the primary tumor and one liver metastasis were examined by immunohistochemistry. Ghrelin and several other hormones and tumor markers ...
Tópico(s): Neuroendocrine Tumor Research Advances
2004 - Oxford University Press | The Journal of Clinical Endocrinology & Metabolism
Guida Maria Portela‐Gomes, Mats Stridsberg, Lars Grimelius, Kjell Öberg, Eva Tiensuu Janson,
Knowledge concerning tissue-specific expression of the five somatostatin receptor subtypes is of great importance in understanding their physiological function. We developed rabbit polyclonal antibodies specific for each human somatostatin receptor subtype and report our results concerning the expression in normal endocrine pancreatic cells. The antibodies were produced by immunizing rabbits with fragments specific for the five cloned somatostatin receptor subtypes. Colocalization of these somatostatin ...
Tópico(s): Receptor Mechanisms and Signaling
2000 - Lippincott Williams & Wilkins | Applied immunohistochemistry & molecular morphology
Guida Maria Portela‐Gomes, Anders Höög,
Insulin-like growth factor II (IGF-II) appears to play an important role during fetal life in cell growth and differentiation in several organs, including the pancreas. In the present study we investigated the cellular localization of IGF-II in human fetal pancreas at 16, 18 and 22 embryonic weeks and compared it with adult pancreas. Single and double immunofluorescence methods were used to study co-localization of IGF-II with the four major islet hormones - insulin, glucagon, somatostatin, pancreatic ...
Tópico(s): Diabetes and associated disorders
2000 - Bioscientifica | Journal of Endocrinology
Guida Maria Portela‐Gomes, Agneta Lukinius, Lars Grimelius,
Synaptic vesicle protein 2 (SV2) is a glycoprotein identified in the nervous system of several species, including man, but its occurrence in the human neuroendocrine (NE) cell system has not been investigated. By using a monoclonal antibody to SV2, immunoreactivities were demonstrated in NE cell types in human gastrointestinal tract, pancreas, anterior pituitary gland, thyroid, parathyroid, and adrenal medulla, and also in chief cells of gastric oxyntic mucosa. Immunoelectron microscopy of pancreatic ...
Tópico(s): Erythrocyte Function and Pathophysiology
2000 - Elsevier BV | American Journal Of Pathology
Amel Guenifi, Guida Maria Portela‐Gomes, Lars Grimelius, Suad Efendić, Samy M. Abdel‐Halim,
Tópico(s): Diabetes and associated disorders
2000 - Springer Science+Business Media | Histochemistry and Cell Biology