Anna Ågren, Agneta Wikman, Margareta Holmström, Anders Östlund, Gustaf Edgren,
Background. Several methods exist for evaluation of hypocoagulation in patients with perioperative bleeding, e.g. thromboelastography (TEG®) and conventional methods (platelet count, aPTT, INR and fibrinogen). Considering the vast experience of conventional methods it is important to investigate how well the methods correspond. Methods. Sixty surgical patients were included prospectively and blood samples were taken perioperatively. TEG® and conventional parameters were analyzed simultaneously. An ...
Tópico(s): Hemodynamic Monitoring and Therapy
2013 - Taylor & Francis | Scandinavian Journal of Clinical and Laboratory Investigation
Anna Pasetto, Lars Frelin, Soo Aleman, Fredrik Holmström, Anette Braß, Gustaf Ahlén, Erwin Daniel Brenndörfer, Volker Lohmann, Ralf Bartenschlager, Matti Sällberg, Antonio Bertoletti, Margaret Chen,
Virus-specific CTL with high levels of functional avidity have been associated with viral clearance in hepatitis C virus (HCV) infection and with enhanced protective immunity. In chronic HCV infection, lack of antiviral CTL is frequently observed. In this study, we aim to investigate novel HCV TCRs that differ in Ag specificity. This involved isolating new HCV-specific murine TCRs that recognize a conserved HLA-A2-restricted CTL epitope within the nonstructural protein (NS) 5A viral protein and comparing ...
Tópico(s): T-cell and B-cell Immunology
2012 - American Association of Immunologists | The Journal of Immunology
Olivia Cintas, Göran Berndes, Annette Cowie, Gustaf Egnell, Hampus Holmström, Gregg Marland, Göran I. Ågren,
Abstract Studies report different findings concerning the climate benefits of bioenergy, in part due to varying scope and use of different approaches to define spatial and temporal system boundaries. We quantify carbon balances for bioenergy systems that use biomass from forests managed with long rotations, employing different approaches and boundary conditions. Two approaches to represent landscapes and quantify their carbon balances – expanding vs. constant spatial boundaries – are compared. We show ...
Tópico(s): Environmental Impact and Sustainability
2016 - Wiley | GCB Bioenergy
Olivia Cintas, Göran Berndes, Annette Cowie, Gustaf Egnell, Hampus Holmström, Göran I. Ågren,
Bioenergy from boreal forests managed for productive purposes (e.g., pulp, timber) is commonly held to offer attractive options for climate change mitigation. However, this view has been challenged in recent years. Carbon balances, cumulative radiative forcing, and average global temperature change have been calculated for a variety of bioenergy management regimes in Swedish forests, and the results support the view that an increased use of forest biomass for energy in Sweden can contribute to climate ...
Tópico(s): Environmental Impact and Sustainability
2015 - Wiley | Wiley Interdisciplinary Reviews Energy and Environment
Gustaf Ahlén, Fredrik Holmström, Anna Gibbs, Mats Alheim, Lars Frelin,
We have investigated the ability of hepatitis C virus non-structural (NS) 3/4A-DNA-based vaccines to activate long-term cell-mediated immune responses in mice. Wild-type and synthetic codon optimized (co) NS3/4A DNA vaccines have previously been shown to be immunogenic in mice, rabbits and humans, although we have very poor knowledge about the longevity of the immune responses primed. We therefore analyzed the functionality of primed NS3/4A-specific immune responses in BALB/c (H-2d) and/or C57BL/6J ( ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2014 - Springer Nature | Gene Therapy
Sepideh Levander, Fredrik Holmström, Lars Frelin, Gustaf Ahlén, Daniel Rupp, Gang Long, Ralf Bartenschlager, Matti Sällberg,
Objective HCV is characterised by its ability to establish chronic infection in hepatocytes and to replicate in the presence of an inflammation. We mimicked this situation in vivo in immune-competent mice by syngeneic transplantation of HCV replicon-containing mouse hepatoma cells. Design A total of 5 million H-2 b positive Hep56.1D cells, carrying a subgenomic genotype (gt) 2a replicon (HCV replicon cells) or stably expressing comparable levels of the HCV NS3/4A protease/helicase complex (NS3/4A hepatoma ...
Tópico(s): HIV Research and Treatment
2017 - BMJ | Gut
Anne Fournillier, Lars Frelin, Emilie Jacquier, Gustaf Ahlén, Anette Braß, Estelle Gerossier, Fredrik Holmström, Kate E. Broderick, Niranjan Y. Sardesai, Jean‐Yves Bonnefoy, Geneviève Inchauspé, Matti Sällberg,
We explored the concept of heterologous prime/boost vaccination using 2 therapeutic vaccines currently in clinical development aimed at treating chronically infected hepatitis C virus (HCV) patients: prime with a DNA-based vaccine expressing HCV genotype 1a NS3/4A proteins (ChronVac-C) and boost with a modified vaccinia virus Ankara vaccine expressing genotype 1b NS3/4/5B proteins (MVATG16643).
Tópico(s): HIV Research and Treatment
2013 - Oxford University Press | The Journal of Infectious Diseases
Gustaf Olsson, Bengt Andersson, Bengt Hellström, Hans Holmström, L.-G. Reinius, Petr Vopatek,
This paper is a summary of a committee working for the Swedish Water and Wastewater Works Association (VAV). The purpose of the report is to present the possibilities today to measure, present and analyze data and control treatment plants. The typical audience is the operator, the process engineer, or the consulting engineer. The methods presented are all known from different disciplines, but are here presented in a form that connects the methods to wastewater treatment operation. Unlike any manual ...
Tópico(s): Water Quality Monitoring Technologies
1989 - Pergamon Press | Water Science & Technology
Antony Chen, Gustaf Ahlén, Erwin Daniel Brenndörfer, Anette Braß, Fredrik Holmström, Margaret Chen, Jonas Söderholm, David R. Milich, Lars Frelin, Matti Sällberg,
The hepatitis C virus (HCV)-specific T cell response in patients with chronic HCV is dysfunctional. In this study, we aimed at restoring immunological function through therapeutic vaccination in a transgenic mouse model with impaired HCV-specific T cell responses due to a persistent presence of hepatic HCV nonstructural (NS)3/4A Ags. The HCV-specific T cells have an actively maintained dysfunction reflected in reduced frequency, impaired cytokine production, and impaired effector function in vivo, ...
Tópico(s): Influenza Virus Research Studies
2011 - American Association of Immunologists | The Journal of Immunology
Fredrik Holmström, Anna Pasetto, Veronica Nähr, Anette Braß, Malte Kriegs, Eberhard Hildt, Kate E. Broderick, Margaret Chen, Gustaf Ahlén, Lars Frelin,
The hepatitis C virus (HCV) nonstructural (NS) 5A protein has been shown to promote viral persistence by interfering with both innate and adaptive immunity. At the same time, the HCV NS5A protein has been suggested as a target for antiviral therapy. In this study, we performed a detailed characterization of HCV NS5A immunogenicity in wild-type (wt) and immune tolerant HCV NS5A-transgenic (Tg) C57BL/6J mice. We evaluated how efficiently HCV NS5A-based genetic vaccines could activate strong T cell responses. ...
Tópico(s): HIV Research and Treatment
2013 - American Association of Immunologists | The Journal of Immunology
Fredrik Holmström, Matti Sällberg, Gustaf Ahlén, Lars Frelin,
Tópico(s): Liver Disease Diagnosis and Treatment
2014 - Elsevier BV | Journal of Hepatology
Artis Becs, Lenka Kuglerová, Dan Bergström, Emma Holmström, Gustaf Egnell,
Tópico(s): Forest Ecology and Biodiversity Studies
2024 - Elsevier BV | Forest Ecology and Management
Åsa Audulv, Kenneth Asplund, Karl‐Gustaf Norbergh,
... ways of understanding the phenomenon under study (Larsson & Holmström 2007). A search of phenomenographic research in Scopus ...
Tópico(s): Interprofessional Education and Collaboration
2011 - Wiley | Journal of Nursing and Healthcare of Chronic Illness
Angela Silveira, Anders Hamsten,
... Svenungsson E, Ågren A, Mobarrez F, Bremme K, Holmström M, Eelde A, Bruzelius M, Elgue G, Wallén ...
Tópico(s): Adipokines, Inflammation, and Metabolic Diseases
2006 - Lippincott Williams & Wilkins | Arteriosclerosis Thrombosis and Vascular Biology
Fredrik Holmström, Margaret Chen, Anangi Balasiddaiah, Matti Sällberg, Gustaf Ahlén, Lars Frelin,
Abstract The hepatitis C virus nonstructural (NS) 3/4A and NS5A proteins are major targets for the new direct-acting antiviral compounds. Both viral proteins have been suggested as modulators of the response to the host cell. We have shown that NS3/4A- and NS5A-specific T cell receptors confer different effector functions and that killing of NS3/4A-expressing hepatocytes is highly dependent on IFN-γ. We here characterize the functional differences in the T cell responses to NS3/4A and NS5A. NS3/4A- ...
Tópico(s): Viral Infections and Immunology Research
2016 - Nature Portfolio | Scientific Reports
Fredrik Holmström, Malte Kriegs, Eberhard Hildt, Matii Sallberg, Gustaf Ahlén, Lars Frelin,
Tópico(s): Immunotherapy and Immune Responses
2012 - Elsevier BV | Journal of Hepatology
Lars Frelin, Gustaf Ahlén, A. Chen, Erwin Daniel Brenndörfer, Anette Braß, Fredrik Holmström, M. Chen, Jonas Söderholm, D.R. Milich, Matti Sällberg,
POSTERSreceiving ABT-267 and 2/14 (14%) receiving placebo thus, similar rates across groups.Only one AE was considered by the investigator to be study drug-related Conclusions: Based on the observed pharmacokinetics and in vitro replicon activity, ABT-267 doses of 5 mg are predicted to have significant antiviral activity.In healthy subjects, steady-state trough concentrations for a 5 mg dose were approximately 10-fold above the in vitro replicon EC 50 .No safety signals have been identified.These ...
Tópico(s): Hepatitis B Virus Studies
2011 - Elsevier BV | Journal of Hepatology
... Britt Wennerholm Eva Larsson, Lene Martin and Gerd Holmström Marita Andersson Grönlund, Eva Aring, Ann Hellström, Magnus ...
Tópico(s): Healthcare Systems and Technology
2003 - Wiley | Acta Ophthalmologica Scandinavica