David M. Novick, Patrizia Farci, Peter Karayiannis, Alvin M. Gelb, Richard J. Stenger, Mary Jeanne Kreek, Howard C. Thomas,
Abstract The hepatitis D virus (HDV; previously called the “delta agent”) is a defective organism which can replicate only in the presence of the hepatitis B virus ( ... liver biopsy, for hepatitis D virus antibody (anti‐HDV). Anti‐HDV was detected in five of eight hepatitis B ... marker in eight of the 12 (67%) anti‐HDV‐positive, HBsAg‐negative patients but in only 14 of 75 (19%) anti‐HDV‐negative, HBsAg‐negative patients (P < 0.005). None of the anti‐HDV‐positive, HBsAg‐negative patients had detectable IgM anti‐ ...
Tópico(s): Liver Disease Diagnosis and Treatment
1985 - Wiley | Journal of Medical Virology
Ferruccio Bonino, Nicola Caporaso, P Dentico, G. Marinucci, L Valeri, Antonio Craxı̀, Antonio Ascione, Giovanni Raimondo, F Piccinino, Giuseppe Rocca, Mario Rizzetto, Paolo Gerardo, Giuseppe Poli, Gianfranco Tappero, B Forzani, Luigi Castellano, Rosalba Suozzo, M. Vinci, Giuseppe Longo, Evangelista Sagnelli,
The prevalence of hepatitis delta virus (HDV) infection was significantly higher among the relatives of 79 carriers of HBsAg with antibody to HDV (index cases) than among relatives of 111 carriers without serological evidence of HDV infection (controls). Antibody to HDV was found in 45 of the 80 (56%) ... controls (P < 0.0001). During follow-up new HDV infection developed in 31% of 13 susceptible carriers ... previously unexposed to the hepatitis B virus had HDV markers in serum or developed this infection during ...
Tópico(s): Liver Disease Diagnosis and Treatment
1985 - Elsevier BV | Journal of Hepatology
J H Lin, Ming‐Fu Chang, Susan C. Baker, Sugantha Govindarajan, M M Lai,
... RNA gel mobility shift assay. Hepatitis delta virus (HDV) RNA bound specifically to the delta antigen in ... high salt concentration. Both genome- and antigenome-sense HDV RNAs and at least two different regions of HDV genomic RNA bound to the delta antigen. Surprisingly, these two different regions of HDV genomic RNA could compete with each other for ... cellular RNA. Since both the genomic and antigenomic HDV RNAs had strong intramolecular complementary sequences, these results ... specific for a secondary structure unique to the HDV RNA. By expressing different subdomains of the delta ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
1990 - American Society for Microbiology | Journal of Virology
Ursula Schlipköter, Antonio Ponzetto, Klaus Fuchs, R. Rasshofer, Sung-Su Choi, Stefan Roos, M. Rapicetta, Michael Roggendorf,
Abstract— Hepatitis delta virus (HDV) superinfection of woodchuck chronic carriers of woodchuck hepatitis virus (WHV) results in acute and chronic disease. The different courses of disease mimicked the outcome of human HDV superinfection, making woodchucks valuable models for clinical studies of HDV. Ten of 11 woodchuck chronic carriers of WHV superinfected with HDV developed acute HDV infection with markers of viral replication in the ... animal (DW128) had no serological markers of acute HDV infection. Nine of 11 (82%) superinfected animals developed ...
Tópico(s): Hepatitis C virus research
1990 - Wiley | Liver International
Jung‐Fa Tsai, Harold S. Margolis, Howard A. Fields, Omana V. Nainan, Wen Yu Chang, Juei‐Hsiung Tsai,
... the role of CIC in hepatitis delta virus (HDV) infection in 54 HBsAg‐negative controls and 85 ... positive patients with chronic hepatitis. The prevalence of HDV markers (HDV antigen and anti‐HD) was 24.70% (21/85). CIC were a common feature of HDV infection with 95.24% of patients having at ... class CIC were statistically higher in patients with HDV infection than in those without ( P = .001 for ... the prevalence of IgG class CIC. Patients with HDV infection also have significantly higher median levels of ... IgG C1q‐CIC ( P = .008). In patients with HDV infection, there was positive correlation between IgM C1q‐ ...
Tópico(s): Blood groups and transfusion
1990 - Wiley | Journal of Medical Virology
Pei‐Jer Chen, Mark Yen‐Ping Kuo, M L Chen, Shu‐Ju Tu, M N Chiu, Hung‐Yi Wu, H C Hsu, Ding‐Shinn Chen,
... clones allowing continuous replication of hepatitis delta virus (HDV), Hep G2, a hepatoblastoma cell line containing no ... a recombinant plasmid containing a tandem trimer of HDV cDNA (driven by the simian virus 40 late ... be integrated into cell chromosomes. Replication of the HDV genome was demonstrated by the presence of not only genomic and antigenomic HDV RNAs but also HDV RNAs in multimeric and circular forms. In addition, ... was documented. Continuous replication and transcription of the HDV genome was thus achieved in these transfected cell ...
Tópico(s): RNA and protein synthesis mechanisms
1990 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Giovanna Fattovich, S. Boscaro, F. Noventa, E. Pornaro, D. Stenico, A. Albertí, Alberto Ruol, G. Realdi,
Serological markers of hepatitis delta virus (HDV) infection were found in 18 (12%) of 146 consecutive patients with chronic hepatitis B, and the characteristics of patients who had antibody to HDV (anti-HDV-positive) were analyzed. During one to 15 years ... histological deterioration was documented in 77% of anti-HDV—positive patients; however, in hepatitis B surface antigen (HBsAg) carriers without HDV infection, histology deteriorated in 30% but improved or ... 01). In seven (70%) of the 10 anti-HDV—positive patients who showed transition from chronic active ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
1987 - Oxford University Press | The Journal of Infectious Diseases
Michael Roggendorf, C. Pahlke, Benjamin Böhm, R. Rasshofer,
... size of proteins associated with hepatitis delta virus (HDV) from serum and liver (human, chimpanzee and woodchuck) in the acute and chronic stages of HDV infection were analysed by immunoblotting. HDV particles in serum were separated from serum proteins by gel filtration and peak fractions of HDV antigens were subjected to PAGE. Immunoblotting with human anti-HDV-positive sera and 125I-labelled Protein A revealed ... structure from liver homogenates by CsCl gradient centrifugation. HDV proteins from such gradients were degraded to a ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
1987 - Microbiology Society | Journal of General Virology
Hong‐Yuan Hsu, Mei‐Hwei Chang, Ding‐Shinn Chen, Chin‐Yun Lee,
... and clinical features of hepatitis D virus infection (HDV) in childhood, total antibody to hepatitis D antigen ( ... showed detectable anti-HD. All three children with HDV coinfection cleared HBsAg and seroconverted to anti-HBs, ... clearance of HBsAg. To identify possible sources of HDV infection, HBV markers and anti-HD in family ... with her HBeAg-negative carrier father, who had HDV superinfection. The infection sources remained undefined in another ... perinatal transmission is not the usual mode of HDV infection in Taiwan. The natural course of either ...
Tópico(s): Hepatitis C virus research
1988 - Elsevier BV | The Journal of Pediatrics
... and for antibody to hepatitis D virus (anti-HDV); 9.7% of the carrier mothers were anti-HDV-positive and the majority of them (94.4%) ... positive. Follow-up of 17 infants of anti-HDV-positive carrier mothers until about 7 months of age showed no evidence of perinatal transmission of HDV. Age-specific prevalence of anti-HDV in 280 Saudi carriers showed that infection with HDV is infrequent in infants and is mainly acquired ... decades of life, which suggests that transmission of HDV is predominantly horizontal. L'antigène Hbe et les ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
1988 - Elsevier BV | Annales de l Institut Pasteur Virologie
W. Blair Geho, Hans C. Geho, John R. Lau, Theophilus J. Gana,
Hepatic-directed vesicle insulin (HDV-I), a novel investigational vesicle (<150 nm diameter) insulin delivery system that carries insulin and a specific hepatocyte-targeting molecule ( ... a portal vein insulin infusion remotely [subcutaneous (SC) HDV-I] and noninvasively (oral HDV-I), has been developed. This review summarizes formulation ... biodistribution, mechanistic, and toxicology studies of predominantly SC HDV-I, in various animal models. Studies conducted to date have confirmed the hepatocyte specificity of HDV and HDV-I and revealed that HDV-I ...
Tópico(s): Liver Disease Diagnosis and Treatment
2009 - SAGE Publishing | Journal of Diabetes Science and Technology
Kalliopi Zachou, Cihan Yurdaydìn, Uta Drebber, George Ν. Dalekos, Andreas Erhardt, Yılmaz Çakaloğlu, Halil Değertekin, Selım Gürel, Stefan Zeuzem, Hakan Bozkaya, Verena Schlaphoff, Hans Peter Dienes, C.‐Thomas Bock, Michael P. Manns, Heiner Wedemeyer,
Abstract Background: Hepatitis delta virus (HDV) causes severe liver disease.To investigate the quantitative HDV-RNA, HBsAg and hepatitis B virus (HBV)DNA levels in correlation to ... biochemical and demographical parameters in patients with chronic HDV infection as similar data in a large series of HDV patients are missing.Eighty HDV patients were recruited in Germany, Turkey and Greece; quantitative determination of HDV-RNA, HBsAg and HBV-DNA was performed by ... HBV test respectively.All patients were infected with HDV-genotype 1. Thirty-five patients (48%) had significant ...
Tópico(s): Hepatitis C virus research
2009 - Wiley | Liver International
Hsiu-Hui Shih, Chiaho Shih, H.-W. Wang, Chien‐Wei Su, I-J. Sheen, Jaw‐Ching Wu,
... the assembly and infection of hepatitis D virus (HDV). The assembly efficiency of genotype 1 HDV is higher than that of genotype 2, whilst ... compromises the assembly of genotype 2 and 4 HDV. This study investigated the influence of proline residues ... delta antigen (HDAg-L) on the assembly of HDV of different genotypes. Expression vectors containing the HDAg-L gene or full-length HDV genome of genotype 1, 2 or 4 were ... leucine residues at position 62. Of the eight HDV genotypes, only genotype 1 has Pro-205 in ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
2009 - Microbiology Society | Journal of General Virology
Ziying Han, Carolina Alves, Severin O. Gudima, John M. Taylor,
ABSTRACT Hepatitis delta virus (HDV) encodes one protein, hepatitis delta antigen (δAg), a 195-amino-acid RNA binding protein essential for the accumulation of HDV RNA-directed RNA transcripts. It has been accepted ... predominantly to the nucleolus in the absence of HDV genome replication while in the presence of replication, δAg facilitates HDV RNA transport to the nucleoplasm and helps redirect ... II) to achieve transcription and accumulation of processed HDV RNA species. This study used immunostaining and confocal ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
2009 - American Society for Microbiology | Journal of Virology
R. Romeo, E. Del Ninno, Maria Grazia Rumi, Antonio Russo, Angelo Sangiovanni, Roberto de Franchis, Guido Ronchi, Massimo Colombo,
Background & Aims Chronic infection with hepatitis Δ virus (HDV) is a risk factor for cirrhosis and hepatocellular ... however, poorly defined. We tracked the course of HDV infection in 299 patients over a mean period ... We analyzed data from patients who had been HDV positive for at least 6 months (230 males; ... from 1978 to 2006 to Maggiore Hospital, Milan. HDV infection was defined by the presence of HDV antigen in liver tissue or serum HDV RNA in anti-HDV/hepititis B surface antigen seropositive patients. At enrollment, ...
Tópico(s): Liver Disease Diagnosis and Treatment
2009 - Elsevier BV | Gastroenterology
Anders Boyd, Karine Lacombe, Patrick Miailhes, Joël Gozlan, Philippe Bonnard, Jean‐Michel Molina, Caroline Lascoux‐Combe, Lawrence Serfaty, Elyanne Gault, Moı̈se Desvarieux, Pierre‐Marie Girard,
... B (HBV), hepatitis C (HCV) and hepatitis D (HDV) viruses in HIV-infected patients have been poorly ... HBV-HCV (39.7, 4.1); 12 HBV-HDV (35.2, 9.9); 12 HBV-HCV-HDV (39.2, 5.2). At inclusion, treatment with ... infection groups. HBV suppression was significantly associated with HDV (aOR = 3.85, 95%CI 1.13-13. ... 04), but marginally associated with HIV-HBV-HCV-HDV (aOR = 2.32, 95%CI 0.94-5.74, P = 0.07). In quad-infection, lower HDV-undetectability (vs HIV-HBV-HDV, P = 0.2) and higher HCV-undetectability (vs ... HBV-HCV = 2.23-5.67, HIV-HBV-HDV = 1.53-15.17]. In treated co-infected ...
Tópico(s): Liver Disease Diagnosis and Treatment
2009 - Wiley | Journal of Viral Hepatitis
... to the discovery of the Hepatitis D Virus (HDV) was the description in Turin, Italy in the ... infected patients, was mainly transmitted by superinfection of HDV on chronic HBV carriers and had the ability ... inhibit the helper HBV. The discovery of the HDV has driven three directions of further research:(1) ... of the replicative and infectious mechanisms of the HDV.(2)The assessment of its epidemiological and medical ... years that have passed since the discovery of HDV. The key to the discovery of the Hepatitis ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
2009 - Elsevier BV | Journal of Hepatology
Yucheng Wang, Chi‐Ruei Huang, Mei Chao, Szecheng J. Lo,
Abstract Background Hepatitis delta virus (HDV) is a defected RNA virus and requires its encoded large antigen (LDAg) to interact with helper viral proteins (HBsAgs) during assembly. ... of the LDAg C-terminus from genotype I HDV to the clathrin heavy chain (CHC), which suggests that this interaction might facilitate HDV assembly. If LDAg binding to clathrin is essential to HDV life cycle, a clathrin box sequence at the ... terminus of LDAg should be conserved across all HDV. However, the C-terminal sequence of LDAg is ...
Tópico(s): HIV Research and Treatment
2009 - BioMed Central | Virology Journal
Edwige Dormond, Pascal Perrier, Amine Kamen,
Helper-dependent adenoviral vectors (HDVs) are the most promising adenoviral vectors for gene therapy treatments as well as vaccination strategies. However, the lack of a robust and efficient strategy to produce the HDV at high titers constitutes a major obstacle hindering ... this promising technology at the clinical level. The HDV production requires a double infection of a recombinase-expressing HEK293 cell line with the HDV and a helper virus (HV). To limit the contamination of viral HDV lots by HV, encapsidation of HV is prevented ...
Tópico(s): CAR-T cell therapy research
2009 - Elsevier BV | Journal of Biotechnology
J.‐C. Wu, Pei‐Jer Chen, Mei‐Ying Kuo, Shin‐Da Lee, Ding‐Shinn Chen, L P Ting,
The hepatitis delta virus (HDV) is a defective virus with a coat composing of the surface antigen of its helper virus, hepatitis B virus (HBV). Replication of HDV in the absence of HBV has been shown in cell cultures by transient transfection of the HDV plasmid. However, the formation and release of HDV virions have not been observed. In this report, ... cell line HuH-7 was transiently cotransfected with HDV and HBV plasmids. The production of monomeric and multimeric antigenomic RNAs of HDV in the transfected cells indicated replication of the ...
Tópico(s): Viral gastroenteritis research and epidemiology
1991 - American Society for Microbiology | Journal of Virology
A. G. Shattock, MAJ JOSEPH T. MORRIS,
... from six companies for detecting hepatitis delta virus (HDV) markers. For detecting hepatitis delta virus antigen (HDAg), ... 8% sensitivity). For the detection of antibody to HDV (anti-HDV) all six commercial enzyme immunoassays were reactive with all 36 anti-HDV-positive specimens that were confirmed in-house. There ... One HDAg-positive specimen gave a false anti-HDV-positive result in the Abbott assay and an ... specificity). For the detection of immunoglobulin M anti-HDV, the Wellcome, Noctech, and Sorin assays agreed for ...
Tópico(s): Viral Infections and Outbreaks Research
1991 - American Society for Microbiology | Journal of Clinical Microbiology
Ton Kos, Anco Molijn, Leen‐Jan van Doorn, Alex van Belkum, Martin A. Dubbeld, Huub Schellekens,
Abstract Hepatitis delta virus (HDV) RNA was isolated from the serum of a chimpanzee acutely infected with hepatitis B virus (HBV) and superinfected with HDV. Interference of HDV with HBV resulted in decreased HBV DNA levels ... the chimpanzee. The complete cDNA sequence of the HDV RNA (5th passage) was determined. Comparison of this ... 4th passage), located in the variable domain of HDV, was highly conserved. The HDV strain used for these infections originated from a human HDV isolate also used for five to seven HDV ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
1991 - Wiley | Journal of Medical Virology
Johnson Y. N. Lau, Heather M. Smith, Kanchan Chaggar, Lucy J. Hansen, Bernard Portmann, Graeme Alexander, Roger Williams,
Abstract Intrahepatic hepatitis D virus (HDV) antigen (HDAg) and serum HDV RNA are excellent markers of active HDV replication but the relation of IgM anti‐HDV to HDV replication and histological activity is less certain. To further elucidate the significance of serum IgM anti‐HDV, 90 paired sera and liver biopsies from 64 patients sero‐positive for total antibody to HDV were analysed for IgM anti‐HDV, intrahepatic HDAg expression, and histological inflammatory activity. IgM ...
Tópico(s): Liver Diseases and Immunity
1991 - Wiley | Journal of Medical Virology
A. Ottobrelli, Alfredo Marzano, Antonina Smedile, S. Recchia, Mauro Salizzoni, C. Cornù, M Lamy, Jean Bernard Otte, Bernard de Hemptinne, André Geubel, M. Grendele, M. Colledan, D Galmarini, G. Marinucci, Cristina Di Giacomo, S. Agnes, Ferruccio Bonino, Mario Rizzetto,
... Italy and Belgium for terminal Hepatitis delta virus (HDV) cirrhosis were investigated.In 22 of the patients, HDV infection recurred.Two patients died of coexisting HDV and hepatitis B virus (HBV) reactivation.Four patients ... died of unrelated causes were found to have HDV without signs of HBV reactivation.Five patients (18%) cleared both HBV and HDV after transplantation with no evidence of hepatitis (mean follow-up, 29 months).In many surviving patients, HDV infection recurred early without signs of HBV reactivation. ...
Tópico(s): Liver Disease and Transplantation
1991 - Elsevier BV | Gastroenterology
Donatella Pacchioni, Francesco Negro, Elisabetta Chiaberge, Mario Rizzetto, Ferruccio Bonino, Gianni Bussolati,
... was used to detect genomic hepatitis delta virus (HDV) RNA in formalin-fixed, paraffin-embedded liver sections ... NISH) procedure. Twenty-three liver samples from chronically HDV-infected patients were studied. Eight liver specimens from humans and chimpanzees without markers of active HDV infection served as negative controls. In three samples, ... nuclear features and with the distribution of the HDV encoded antigen, HDAg, as detected by direct immunofluorescence. All samples from HDV-infected patients were positive for HDV RNA by ...
Tópico(s): Viral gastroenteritis research and epidemiology
1992 - Elsevier BV | Human Pathology