National Bureau of Economic Research (NBER)
A.M. Rao, Andreas Weber, S. Gollamudi, R.A. Soni,
... System (UMTS) with its high speed packet access (HSPA) enhancements is currently being deployed as the primary ... UMTS, with significant performance improvement targets compared to HSPA. The fact that LTE is not backwards compatible with HSPA spurned the introduction of the HSPA evolution (HSPA+) effort in 3GPP to protect current operator investments in HSPA. HSPA+ provides a framework for HSPA enhancements with the goal of providing performance similar ... to meet the desired performance improvements compared to HSPA, and then describe the key features of HSPA+ ...
Tópico(s): Advanced MIMO Systems Optimization
2009 - Wiley | Bell Labs Technical Journal
Sebastian Suerbaum, Jean‐Michel Thiberge, Imad Kansau, Richard L. Ferrero, Agnès Labigne,
... to belong to a bicistronic operon including the hspA and hspB genes. In Escherichia coli. the constitutive expression of the hspA and hspB genes was initiated from a promoter ... was thus acquired during the cosmid cloning process. hspA and hspB encoded polypeptides of 118 and 545 ... sequence comparison studies revealed that, although H. pylori HspA and HspB proteins were highly similar to their bacterial homologues, the H. pylori HspA featured a striking motif at the C ‐terminus. ... observed. This suggested a close interaction between the HspA and HspB proteins and the urease enzyme, and ...
Tópico(s): Clostridium difficile and Clostridium perfringens research
1994 - Wiley | Molecular Microbiology
Paweł Stocki, Nicholas J. Morris, Christian Preisinger, Xiao N. Wang, Walter Kölch, Gabriele Multhoff, Anne M. Dickinson,
Heat shock protein 70 (HSPA) is a molecular chaperone which has been suggested to shuttle human leukocyte antigen (HLA) epitope precursors from the proteasome to ... Despite the reported observations that peptides chaperoned by HSPA are an effective source of antigens for cross- ... this study, we investigated the possible involvement of HSPA in HLA class I or class II antigen ... analysed the antigenic potential of the associated peptides. HSPA was purified from CCRF-CEM and K562 cell ... 44 different peptides which were co-purified with HSPA. The affinity of the identified peptides to two ...
Tópico(s): Hepatitis B Virus Studies
2010 - Springer Science+Business Media | Cell Stress and Chaperones
Harri Holma, Antti Toskala, Karri Ranta‐aho, Juho Pirskanen,
High-speed packet access (HSPA) was included in third generation partnership project (3GPP) releases 5 and 6 for downlink and for uplink. The 3GPP release 7 offers a number of HSPA enhancements, providing major improvements to the end-user ... this paper. Release 7 also is known as HSPA evolution or HSPA+. The HSPA+ downlink peak bit rate can be increased to ... release 6 to two in release 7. The HSPA flat architecture in release 7 is similar to ... LTE), thus enabling the later, smooth evolution from HSPA to LTE. LTE will be specified as part ...
Tópico(s): Advanced MIMO Systems Optimization
2007 - Institute of Electrical and Electronics Engineers | IEEE Communications Magazine
Mugen Peng, Xiang Zhang, Wenbo Wang, Hsiao‐Hwa Chen,
... in physical layer, the high speed packet access (HSPA) evolution (HSPA+) has been proposed for a smooth migration. HSPA+ is an effort to further improve radio performance of HSPA, and wideband CDMA (WCDMA) HSPA+ and time division-synchronous CDMA (TD-SCDMA) HSPA+ (TD-HSPA+) are two HSPA+ systems evolving from WCDMA and TD-SCDMA, respectively. Different from WCDMA HSPA+ system, TD-HSPA+ system utilizes eight antennas at ...
Tópico(s): Wireless Communication Networks Research
2011 - Institute of Electrical and Electronics Engineers | IEEE Systems Journal
Imad Kansau, Florent Guillain, Jean‐Michel Thiberge, Agnès Labigne,
... gene encoding this protein (heat‐shock protein A, HspA) was recently cloned and it was shown to be unique in structure. H. pylori HspA consists of two domains: the N‐terminal domain ( ... domain. Various recombinant proteins consisting of the entire HspA polypeptide, the A domain, or the B domain ... MBP‐fused proteins allowed us to conclude that HspA binds nickel ions by means of its C‐terminal domain. HspA exhibited a high and specific affinity for nickel ... zinc, cobalt). Equilibrium dialysis experiments revealed that MBP–HspA binds nickel ions with an apparent dissociation constant ( ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
1996 - Wiley | Molecular Microbiology
S. Imamura, Munehiko Asayama, Makoto Shirai,
... inducible psbA2 or the dark-/heat-inducible lrtA/hspA promoters, respectively. Further analysis using a mutant psbA2 ... factor contributes to the transcription of heat-inducible hspA or dark-inducible lrtA genes (Imamura et al. ... bp) AGCTTTACAAAACTCTCATTAATCCTTTAGACTAAGTTTa 329 nt pKK-PA PCC 6803 hspA −35 & −10 (15 bp) CCGAATCTAACCTGGAAGGGGAAATTTTAAGATAGAACCa 345 nt pKK- ... factors was examined in vitro with heat-shock hspA and dark-induced lrtA promoters, which are specifically ... each promoter were summarized in Table 1. The hspA promoter is the E. coli consensus-type promoter ...
Tópico(s): Microbial Community Ecology and Physiology
2004 - Wiley | Genes to Cells
Sam Khalouei, Ari M. Chow, Ian R. Brown,
... cells, localization of two inducible members of the HSPA ( HSP 70) family was investigated, namely HSPA 6 ( HSP 70B') and HSPA 1A ( HSP 70‐1). Following heat shock, yellow fluorescent protein ( YFP )‐tagged HSPA 6 and HSPA 1A proteins localized to nuclear speckles that are ... of the nucleolus (identified by nucleophosmin). Subsequently, YFP ‐ HSPA 6 protein, but not YFP ‐ HSPA 1A, localized to the periphery of nuclear speckles that are sites of RNA transcription. The HSPA 6 gene is present in the human genome ... are lacking a potentially protective member of the HSPA family. image Potential stress‐sensitive sites were identified ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2014 - Wiley | Journal of Neurochemistry
Christine Kirunga Tashobya, Valéria Silveira, Freddie Ssengooba, Juliet Nabyonga‐Orem, Jean Macq, Bart Criel,
... attributes for a 'good' health system performance assessment (HSPA) framework from literature and experiences in different contexts ... Literature review to identify relevant attributes for a HSPA framework; attribute validation for LICs in general, and ... and, finally, review of a selection of existing HSPA frameworks using these attributes. Literature review yielded six key attributes for a HSPA framework: an inclusive development process; its embedding in ... learnt from the review of a selection of HSPA frameworks using the attributes include: it is possible ...
Tópico(s): Healthcare Policy and Management
2014 - BioMed Central | Globalization and Health
Jun Wu, Xuehan Li, Lei Huang, Surong Jiang, Fei Tu, Xiaojin Zhang, He Ma, Rongrong Li, Chuanfu Li, Yuehua Li, Zhengnian Ding, Li Liu,
Abstract Heat shock protein A12B ( HSPA 12B) is a newly discovered member of the HSP 70 protein family. This study investigated the effects of HSPA 12B on lipopolysaccharide ( LPS )‐induced inflammatory responses in ... inflammatory model was induced by LPS . Overexpression of HSPA 12B in HUVEC s was achieved by infection with recombinant adenoviruses encoding green fluorescence protein‐ HSPA 12B. Knockdown of HSPA 12B was achieved by si RNA technique. Twenty ... tumour necrosis factor‐α was measured by ELISA . HSPA 12B suppressed LPS ‐induced HUVEC permeability and reduced ...
Tópico(s): Connexins and lens biology
2014 - Wiley | Journal of Cellular and Molecular Medicine
Xiaojuan Zhang, Shuying Feng, Zhitao Li, Yanming Feng,
... H. pylori). Methods. After L. lactis NZ3900/pNZ8110-hspA was constructed, growth curves were plotted to study ... growth of recombinant L. lactis was affected after hspA was cloned into L. lactis and whether the ... Western blot were adopted, respectively, to detect the HspA expressed by recombinant L. lactis and its immunoreactivity. ... observed from the growth curve after exogenous gene hspA was cloned into L. lactis NZ3900; different concentrations ... of Nisin inhibited the growth of NZ3900/pNZ8110-hspA except 10 ng/mL Nisin. No HspA strip ...
Tópico(s): Escherichia coli research studies
2015 - Hindawi Publishing Corporation | Gastroenterology Research and Practice
Enders K. Ng, Stuart A. Thompson, Guillermo I. Pérez‐Pérez, Imad Kansau, Arie van der Ende, Agnès Labigne, Joseph J.�Y. Sung, S. C. Sydney Chung, Martin J. Blaser,
... an unusual GroES homolog, heat shock protein A (HspA). The present study was aimed at an assessment of the serological response to HspA in a group of Chinese patients with defined ... diversity is present in the nucleotide sequences encoding HspA in isolates from these patients. Serum samples collected ... immunoglobulin G (IgG) serologic response to H. pylori HspA by an enzyme linked immunosorbant assay. HspA-encoding nucleotide sequences in H. pylori isolates from 14 patients (7 seropositive and 7 seronegative for HspA) were analyzed by PCR and direct sequencing of ...
Tópico(s): Galectins and Cancer Biology
1999 - American Society for Microbiology | Clinical and Diagnostic Laboratory Immunology
Yanlin Zhao, Chang Liu, Jiali Liu, Qiuyue Kong, Yu Mao, Hao Cheng, Nan Li, Xioajin Zhang, Chuanfu Li, Yuehua Li, Li Liu, Zhengnian Ding,
... Stroke is the leading cause of disability worldwide. HSPA 12B, a heat‐shock protein recently identified expression ... artery occlusion in transgenic mice with overexpression of HSPA 12B ( HSPA 12B Tg) and wild‐type littermates ( WT ). HSPA 12B Tg mice demonstrated a significant higher survival ... locomotor activity and decreased anxiety were detected in HSPA 12B Tg mice compared with WT controls within ... stroke. Stroke‐induced hippocampal degeneration was attenuated in HSPA 12B Tg mice examined at day 28 post‐ ...
Tópico(s): Heat shock proteins research
2018 - Wiley | Journal of Cellular and Molecular Medicine
Ianko Iankov, Cheyne Kurokawa, Kimberly Viker, Steven I. Robinson, Arun Ammayappan, Eleni Panagioti, Mark J. Federspiel, Evanthia Galanis,
... oncolytic virotherapy. Helicobacter pylori heat shock protein A (HspA) is a bacterial heat shock chaperone with essential ... immunogenicity of an attenuated MV strain encoding the HspA transgene (MV-HspA). MV-HspA showed faster replication within 48 h of infection ... 106 50% tissue culture infectious dose (TCID50) MV-HspA significantly improved survival in an ovarian cancer xenograft ... versus 27 days for the control group. The HspA transgene induced a humoral immune response in measles- ... of nine animals developed a long-term anti-HspA antibody response with titers of 1:400 to ...
Tópico(s): Virus-based gene therapy research
2020 - Elsevier BV | Molecular Therapy — Oncolytics
Subhash Medhi, Manash Pratim Sarma, Muhammad Asim, Premashis Kar,
... study the role of heat shock protein A1L ( HSPA 1L) and A1B ( HSPA 1B) polymorphisms and subsequent risk of hepatocellular carcinoma ( ... 200 healthy controls. Genomic DNA was typed for HSPA 1L2437 and HSPA 1B1267 SNP using polymerase chain reaction with restriction ... increased the risk of HCC . The frequencies of HSPA 1L BB genotype and B allele in HCC ... 6.32; P = 0.010]. The frequency of HSPA 1B genotype in the homozygous state was more ... a higher frequency of the B allele of HSPA 1B than healthy controls [ OR : 3.95; P = ...
Tópico(s): Liver Disease Diagnosis and Treatment
2012 - Wiley | Journal of Viral Hepatitis
Kouji Kojima, Hitoshi Nakamoto,
The hspA gene encodes a small heat-shock protein in the thermophilic cyanobacterium Synechococcus vulcanus. To gain insight into the post-transcriptional regulation, hspA was expressed in Escherichia coli. HspA was induced upon a temperature upshift from 30 to 42 degrees C, although the hspA transcription in E. coli occurred constitutively at both ... 42 degrees C. Neither replacement of the native hspA promoter with the lacZ promoter nor the addition ... of a series of the transcriptional and translational hspA-lacZ fusions confirmed the constitutive transcription, and demonstrated ...
Tópico(s): Protein Structure and Dynamics
2005 - Elsevier BV | Biochemical and Biophysical Research Communications
Guillermo I. Pérez‐Pérez, JM Thiberge, A Labigne, Martin J. Blaser,
Heat-shock protein A (HspA) is a GroES homolog in Helicobacter pylori. Using a recombinant HspA-maltose-binding protein fusion, the serologic response to HspA were determined. For 139 H. pylori-uninfected persons, responses to HspA were low-level or absent. In a survey ... no relationship between clinical outcome of infection and HspA seropositivity. Using paired sera obtained from 39 subjects ( ... 1 years apart), the stability of seroresponsiveness to HspA was examined. For 34 persons there was no ... 25 initially seronegative persons seroconverted. The hypothesis that HspA seropositivity was related to patient age was examined ...
Tópico(s): Toxin Mechanisms and Immunotoxins
1996 - Oxford University Press | The Journal of Infectious Diseases
Nataša Perić, Maria M. Hofmarcher-Holzhacker, Judit Simon,
... effectiveness, efficiency and equity. Health system performance assessment (HSPA) has received considerable attention at EU level as ... current state and developments in the field of HSPA relevant in the EU context and by this aid the navigation in the growing HSPA system, understand the available tools and identify opportunities ... the literature on initiatives in the field of HSPA at EU level was carried out. Key literature ... between the different actions and actors involved in HSPA was developed. In addition, expert opinion was sought ...
Tópico(s): Primary Care and Health Outcomes
2017 - BioMed Central | Archives of Public Health
Yoon-Jin Lee, Gil Hong Park, Hye-Nyun Cho, Chul-Koo Cho, Young-Mee Park, Su‐Jae Lee, Yun‐Sil Lee,
... RIF Cells Transfected with Hspb1 (Hsp25) or Inducible Hspa (Hsp70). Radiat. Res. 157, 371–377 (2002).An ... RIF cells did. Since the expression of inducible Hspa (also known as Hsp70) and Hspb1 (also known ... these two cell lines, the role of inducible Hspa and Hspb1 in the adaptive response was examined. When RIF cells were transfected with inducible Hspa or Hspb1, both radioresistance measured by clonogenic assays ... acquired by these two cell lines. The inducible Hspa transfectant showed a more pronounced adaptive response than ...
Tópico(s): Effects of Radiation Exposure
2002 - Radiation Research Society | Radiation Research
Fung Po Tso, Jin Teng, Weijia Jia, Dong Xuan,
... a 3.5G cellular standard usually abbreviated as HSPA) networks in Hong Kong via extensive field tests. ... has largely negative impacts on the performance of HSPA networks, as fast-changing wireless environment causes serious ... and thereby exposed new features of the mobile HSPA networks, which contradict commonly held views. We surprisingly ... triggering and final results of handoffs in mobile HSPA networks are unpredictable and often inappropriate, thus calling ... that mobility is a double-edged sword for HSPA networks. To the best of our knowledge, this ...
Tópico(s): Wireless Networks and Protocols
2011 - Institute of Electrical and Electronics Engineers | IEEE Transactions on Parallel and Distributed Systems
Julio Rodríguez‐Romero, Luis M. Corrochano,
Summary The gene hspA for the heat‐shock protein HSP100 is induced by blue light and heat shock in the zygomycete fungus Phycomyces ... investigated the molecular details of the regulation of hspA gene transcription. We have cloned 1.9 kb of hspA upstream DNA sequence and identified many DNA segments ... We have identified several gene products required for hspA photoactivation and found that they are also required ... carotene, or a chemical derivative, is required for hspA gene photoactivation. The activation of hspA after blue ...
Tópico(s): Plant biochemistry and biosynthesis
2006 - Wiley | Molecular Microbiology
GT Sharma, Amar Nath, Shiv Prasad, Sumit Singhal, Neeraj Singh, NE Gade, PK Dubey, G. Saikumar,
... shock proteins (HSPs), amongst them HSP70, encoded by HSPA‐1A gene, is most abundant and highly conserved ... was designed to study the quantitative expression of HSPA‐1A mRNA in immature oocytes (IMO), matured oocytes ( ... in vivo and in vitro culture conditions. Further, HSPA‐1A gene sequence was analysed to determine its ... IVP and 55 IVD buffalo embryos. Expression of HSPA‐1A was found in oocytes and throughout the ... compared to IVD embryos. Phylogenetic analysis of bubaline HSPA‐1A revealed that it shares 91–98% identity ...
Tópico(s): Effects of Environmental Stressors on Livestock
2012 - Wiley | Reproduction in Domestic Animals
Hongxin Zhang, Xinsheng Zhang, Ming Liu, Jianye Zhang, Yanjie Li, Chengchao Zheng,
... as factories to produce antigens of biotechnological interest. HspA (heat‐shock protein A) is an effective antigen ... employed to amplify the gene fragment of the HspA from H. pylori chromosomal DNA. The pGEM‐T ... the insertion of the gene fragment of the HspA , and the vector pBI121 was used to construct ... of mice with transgenic tobacco extracts containing the HspA protein elicited anti‐HspA serum antibody that specifically bound to the purified bacterial HspA protein. The present study, using transgenic tobacco plants, ...
Tópico(s): Plant tissue culture and regeneration
2005 - Wiley | Biotechnology and Applied Biochemistry
Nataša Perić, Maria M. Hofmarcher, Judit Simon,
... on the prioritization of health system performance assessment (HSPA) indicators. Selected "leading" or "headline" HSPA indicators may provide early warnings of policy impacts. ... the availability of reported indicators by looking at HSPA initiatives in Member States (MSs) of the European ... to elicit preferences from a wide range of HSPA experts on i) the most relevant HSPA domain(s), i.e. access, efficiency, quality of ... as the top headline indicator for domain equity.HSPA indicators from different initiatives largely overlap and public ...
Tópico(s): Healthcare Systems and Reforms
2018 - BioMed Central | Archives of Public Health
... r 18 000 and heat shock protein A (HspA) from Helicobacter pylori (H.pylori) in E. coli ... H. pylori infection. METHODS:The target gene of HspA was amplified from H. pylori chromosome by PCR, ... enzyme Hind III and BamH I simultaneously.pET32a(+)/ HspA and Omp 18 were recovered from 1 % agarose ... digested viscidity end.The recombinant plasmid of pET32a(+)/HspA/Omp 18 was transformed and expressed in E. ... H. pylori M r 18 000 OMP and HspA was cloned and expressed successfully.The results obtained ...
Tópico(s): Veterinary medicine and infectious diseases
2003 - Baishideng Publishing Group | World Journal of Gastroenterology
Shujian Cun, Hongyan Li, Ruiguang Ge, Marie C.M. Lin, Hongzhe Sun,
HspA, a member of the GroES chaperonin family, is a small protein found in Helicobacter pylori with ... filtration chromatography and native electrophoresis demonstrated that apo-HspA exists as a heptamer in solution. Unexpectedly, binding ... bismuth may interfere with the biological functions of HspA. When cultured in Ni(2+)-supplemented M9 minimal ... Escherichia coli BL21(DE3) cells expressing wild-type HspA or the C-terminal deletion mutant clearly indicated ... hosts were grown in Bi(3+)-supplemented medium. HspA may therefore play a dual role: to facilitate ...
Tópico(s): Trace Elements in Health
2008 - Elsevier BV | Journal of Biological Chemistry
Jurre Hageman, Harm H. Kampinga,
... library for the expression of HSP110/HSPH, HSP70/HSPA and HSP40/DNAJ members. First, the heat shock ... In addition, we describe the generation of a HSPA/DNAJ overexpression library and tested the effect of different fusion tags on HSPA and DNAJ members using different techniques for measuring ... expression library containing most members from the HSPH, HSPA, DNAJA and DNAJB families which will be useful ...
Tópico(s): Protein Structure and Dynamics
2008 - Springer Science+Business Media | Cell Stress and Chaperones
Silvina B. Nadin, Mayra L. Sottile, Maria Magdalena Montt Guevara, Gisel V. Gauna, P. Daguerre, Marcela Leuzzi, Francisco E. Gago, Jorge E. Ibarra, F. Darío Cuello-Carrión, Daniel R. Ciocca, Laura M. Vargas‐Roig,
... immunohistochemistry, the expression of HSP27 (HSPB1) and HSP70 (HSPA) in serial biopsies from locally advanced breast cancer ... methotrexate, and 5-fluorouracil. High nuclear HSPB1 and HSPA expressions were found in invasive cells after DOX/ ... of the HSPs. Infiltrating lymphocytes showed high nuclear HSPA (P < 0.01) levels at postchemotherapy. No correlations ... high nuclear (>31 % of the cells) and cytoplasmic HSPA expressions (>11 % of the tumor cells) were associated ... and P = 0.0118, respectively). We conclude that HSPA expression may be a useful prognostic marker in ...
Tópico(s): Effects of Radiation Exposure
2013 - Springer Science+Business Media | Cell Stress and Chaperones