National Bureau of Economic Research (NBER)
Cornelia Kampfer, S. Spillner, Katrin Spinnler, Julia Schwarzer, Claudio Terradas, Roberto Ponzio, Elisa Puigdomènech, Oscar Levalle, Frank M. Köhn, María Eugenia Matzkin, Ricardo S. Calandra, Mónica Beatriz Frungieri, Artur Mayerhofer,
... the human testis, from men with normal spermatogenesis (HTPCs) and infertile patients (HTPC‐Fs), previously allowed us to identify an end ... which were identified by RT‐PCR/sequencing in HTPCs and HTPC‐Fs and whole testes. Catalase, DJ‐1, peroxiredoxin ... or DJ‐1 levels, are significantly higher in HTPC‐Fs than in HTPCs. This particular difference is correlated with the improved ability of HTPC‐Fs to handle ROS, which became evident when ... H 2 O 2 induced stronger responses in HTPCs than in HTPC‐Fs, which correlates with the ...
Tópico(s): Reproductive Biology and Fertility
2012 - Wiley | Andrology
M. Adam, Julia Schwarzer, Frank M. Köhn, Leena Strauss, Matti Poutanen, Artur Mayerhofer,
... by RT–PCR. Primary human testicular peritubular cells (HTPCs), which originate from normal and fibrotically altered testes (HTPC-Fs), were studied by qRT–PCR, western blotting, ... with impaired spermatogenesis. Mirroring the situation in vivo, HTPC-Fs secreted more DCN than HTPCs (P< 0.05). In contrast to HTPCs, HTPC-Fs also responded to the main MC product, ... 05). Several GF receptors (GFRs) are expressed by HTPCs and HTPC-Fs. DCN acutely increased intracellular Ca2+-levels and ... PDGF) and EGF induced strong mitogenic responses in HTPC/-Fs, actions that were blocked by DCN, suggesting ...
Tópico(s): Proteoglycans and glycosaminoglycans research
2011 - Oxford University Press | Human Reproduction
Christine Mayer, M. Adam, L. Glashauser, Kim-Gwendolyn Dietrich, Julia Schwarzer, Frank M. Köhn, Leena Strauss, H. Welter, Matti Poutanen, Artur Mayerhofer,
... wall of seminiferous tubules, human testicular peritubular cells (HTPCs), orchestrate inflammatory events and that Toll like receptors ( ... ECM) of this wall are involved. In cultured HTPCs we detected TLRs, including TLR2. A TLR-2 ... 1 (MCP-1) and pentraxin 3 (PTX3) in HTPCs. The ECM-derived proteoglycan biglycan (BGN) is secreted by HTPCs and may be a TLR2-ligand at HTPCs. In support, recombinant human BGN increased PTX3, MCP-1 and IL-6 in HTPCs. Variable endogenous BGN levels in HTPCs derived from different men and differences in BGN ...
Tópico(s): Sperm and Testicular Function
2016 - Nature Portfolio | Scientific Reports
... least explored testicular cells.Human testicular peritubular cells (HTPCs) can be isolated from small testicular fragments of ... to male (in)fertility.Human testicular peritubular cells (HTPCs) retain contractile abilities in vitro and secrete many ... cells, androgens enhanced GDNF production, but not in HTPCs. Rather, AR activation increased the levels of AR ... PG)-synthesizing enzyme (PTGS1 = COX1), we found that HTPCs secrete PGE2 . COX1, and PGE2 receptors (EP1, 2, ... cells in situ, supporting in vivo relevance. In HTPCs, activation of EP1/4 increased GDNF and a ...
Tópico(s): Hormonal and reproductive studies
2019 - Wiley | Andrology
Katrin Spinnler, F.‐M. Köhn, U. Schwarzer, Artur Mayerhofer,
... the secretory repertoire of human testicular peritubular cells (HTPCs), which includes nerve growth factor. Whether peritubular cells ... isolated peritubular cells from men with normal spermatogenesis (HTPCs) and impaired spermatogenesis and testicular fibrosis (HTPC-Fs). Human testicular biopsies and peritubular cells in ... the peritubular and tubular but not interstitial compartments. HTPCs and HTPC-Fs lack FSH- and LH-receptors but express ... no effect on the secretion of GDNF by HTPCs or HTPC-Fs. Peritubular cells in testes of ...
Tópico(s): Testicular diseases and treatments
2010 - Oxford University Press | Human Reproduction
Puqiang He, Jian Tu, Yi Yang, Hui Huang, Buming Chen, Chao Gao, Yapeng He, Guo Zhong-cheng,
... advancement of LCBs. Herein, hierarchical tubular porous carbon (HTPC) with high specific surface area derived from mulberry ... specific surface area, reduces the graphitization degree of HTPC, whereas intrinsic tubular structure would collapse under high activation temperature. The critical function of HTPC as negative additive in LCBs is exhaustively elaborated, where the LCBs with 1.2 % HTPC deliver a discharge capacity of 165.4 mAh ... performance improvement is attributed to the cooperation of HTPC with excellent conductivity, high specific surface, rich functional ...
Tópico(s): Advanced Battery Materials and Technologies
2023 - Elsevier BV | Journal of Energy Storage
Nicholas A. Castillo, W. Ryan James, Rolando O. Santos, Ryan J. Rezek, Daniel Červený, Ross E. Boucek, Aaron J. Adams, Tony L. Goldberg, Lewis J. Campbell, Addiel U. Perez, Juan J. Schmitter‐Soto, Jonathan P. Lewis, Jerker Fick, Tomas Brodin, Jennifer S. Rehage,
... metric based on the human therapeutic plasma concentration (HTPC), comparing measured concentrations to a threshold of 1/3 the HTPC for each pharmaceutical. Every bonefish had at least ... was detected in exceedance of the 1/3 HTPC threshold in 39% of bonefish, with an average ... did not (23 pharmaceuticals exceeded the 1/3 HTPC threshold). The most common pharmaceuticals were venlafaxine (43 ... systems and shows the utility of applying the HTPC to assess the potential for pharmacological effects, and ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2023 - Elsevier BV | Chemosphere
Annika Missel, Lena Walenta, Katja Eubler, Nadine Mundt, Hanna Heikelä, Ulrich Pickl, Matthias Trottmann, Bastian Popper, Matti Poutanen, Leena Strauss, Frank‐Michael Köhn, Lars Kunz, Marc Spehr, Artur Mayerhofer,
... inflammatory cytokine production by human testicular peritubular cells (HTPCs). The ectonucleotidases ENTPD1 and NT5E degrade ATP and ... were found in situ and in vitro in HTPCs. Malachite green assays confirmed enzyme activities in HTPCs. Pharmacological inhibition of ENTPD1 (by POM-1) significantly ... three of the four known adenosine receptors in HTPCs. One, A2B, was also found in situ in ... receptor. In mouse peritubular cells, in contrast to HTPCs, all four of the known adenosine receptors were ...
Tópico(s): Prenatal Substance Exposure Effects
2021 - Oxford University Press | Molecular Human Reproduction
Nina Schmid, Annika Missel, Stoyan Petkov, Jan B. Stöckl, Florian Flenkenthaler, Georg J. Arnold, Thomas Fröhlich, Rüdiger Behr, Artur Mayerhofer,
... Previous studies employing isolated human testicular peritubular cells (HTPCs) indicated that their roles in the testis go ... jacchus , share a high degree of homology with HTPCs. However, like their human counterparts these cells age ... whether they respond in a similar way as HTPCs, iMKTPCs were challenged with forskolin (FSK) and ATP. As HTPCs, they showed increased expression level of the StAR ... IL1B ; CCL7 ), as it is the case in HTPCs. Finally, we confirmed that iMKTPCs can efficiently be ...
Tópico(s): Hormonal and reproductive studies
2020 - Bioscientifica | Reproduction
Nina Schmid, Jan B. Stöckl, Florian Flenkenthaler, Kim-Gwendolyn Dietrich, Julia Schwarzer, F.‐M. Köhn, Charis Drummer, Thomas Fröhlich, Georg J. Arnold, Rüdiger Behr, Artur Mayerhofer,
... for the study of human testicular peritubular cells (HTPCs)?MKTPCs can be isolated and propagated in vitro, ... of 0.78) overlaps with the proteome of HTPCs.Smooth-muscle-like peritubular cells form the wall ... qualify as a translational model we also examined HTPCs from seven men and compared the proteomes of ... Mass-spectrometry-based analyses of the MKTPC and HTPC proteomes were performed.We established a method for ... cells in the human testis.Previous studies with HTPCs from patients revealed a degree of heterogeneity, possibly ...
Tópico(s): Prostate Cancer Treatment and Research
2018 - Oxford University Press | Molecular Human Reproduction
Azam Dalman, Mehdi Totonchi, Mojtaba Rezazadeh Valojerdi,
... and their differentiation into human theca progenitor cells (hTPCs). hTSCs were isolated from the theca layer of ... chondrocyte‐like cells. The cells also differentiated into hTPCs. The hTSCs were morphologically similar to human fibroblast ... F12 culture medium results in the presence of hTPC markers. hTPCs displayed lipid droplets, appropriate gene expression, and secreted ... the ability to differentiate into mesenchymal lineages and hTPCs. This study may provide a novel in vitro ...
Tópico(s): Mesenchymal stem cell research
2018 - Wiley | Journal of Cellular Biochemistry
Christoph Schell, M. Albrecht, Christine Mayer, Julia Schwarzer, Mónica Beatriz Frungieri, Artur Mayerhofer,
... we primarily studied cultured human testicular peritubular cells (HTPCs), isolated from adult human testes. Having established that HTPCs express TNF-α receptors 1 and 2 and ... and ELISA techniques to study phenotypic characteristics of HTPCs and actions of TNFα. We found that HTPCs express the nerve growth factor gene and TNF- ... protein-1 was identified as a product of HTPCs, which was regulated by TNF-α in a ...
Tópico(s): Signaling Pathways in Disease
2008 - Oxford University Press | Endocrinology
... growing interest recently in high-temperature proton conductors (HTPCs). This chapter discusses the basic theory of the ... the reactions occurring at the electrodes when an HTPC electrolyte is used. A critical and comprehensive review of the state-of- the-art HTPC electrolytes is then presented, together with a review ... of the art in electrodes for application with HTPC electrolytes. SOFC applications for HTPC electrolytes are also reviewed and discussed.
Tópico(s): Fuel Cells and Related Materials
1985 - Elsevier BV | Advances in Engineering Software (1978)
Annika Liebich, Nina Schmid, Christina Koupourtidou, Carola Herrmann, Kim-Gwendolyn Dietrich, H. Welter, Jovica Ninkovic, Artur Mayerhofer,
... RNA sequencing of cultured human testicular peritubular cells (HTPCs), which had been isolated from testicular samples of ... and recently published ex vivo data indicates that HTPCs are a highly adequate cellular model to define ... Thus, based on the expression of several markers, HTPCs can be classified as testicular smooth muscle cells. ... g., the ability to produce retinoic acid. Therefore, HTPCs allow us to define the signature(s) and ...
Tópico(s): Reproductive Biology and Fertility
2022 - Multidisciplinary Digital Publishing Institute | Cells
Kezhen Yin, Jingwei Zhang, Zhenpeng Li, Jingxing Feng, Ci Zhang, Xinyue Chen, Andrew Olah, D. E. Schuele, Lei Zhu, Eric Baer,
... technique. High glass transition temperature ( T g ) polycarbonate (HTPC, T g = 165 °C) and polysulfone (PSF, T ... PVDF system was more thermally stable than the HTPC/PVDF system because of the higher T g ... PSF. At temperatures lower than 170 °C, the HTPC/PVDF system exhibited comparable breakdown strength and hysteresis ... orientation. These results demonstrate that PSF/PVDF and HTPC/PVDF systems are applicable for high temperature film ...
Tópico(s): Ferroelectric and Piezoelectric Materials
2019 - Wiley | Journal of Applied Polymer Science
Nina Schmid, Florian Flenkenthaler, Jan B. Stöckl, Kim-Gwendolyn Dietrich, Frank M. Köhn, Julia Schwarzer, Lars Kunz, Manja Luckner, Gerhard Wanner, Georg J. Arnold, Thomas Fröhlich, Artur Mayerhofer,
... age is not understood. Human testicular peritubular cells (HTPCs) transport sperm, contribute to the spermatogonial stem cell ( ... studied in vitro . Consequences of replicative senescence of HTPCs were evaluated to gain partial insights into human testicular aging. To this end, early and advanced HTPC passages, in which replicative senescence was indicated by ... typical for senescent cells, in general. To examine HTPC-specific changes, focused ion beam scanning electron microscopy ( ...
Tópico(s): Peptidase Inhibition and Analysis
2019 - Nature Portfolio | Scientific Reports
S. Windschüttl, Cornelia Kampfer, Christine Mayer, Florian Flenkenthaler, Thomas Fröhlich, Julia Schwarzer, Frank M. Köhn, Henryk F. Urbanski, Georg J. Arnold, Artur Mayerhofer,
... proteomic study using cultured human testicular peritubular cells (HTPCs) i.e. the cells, which form this wall, ... that human endothelial cells (HUVECs) are repulsed by HTPCs. The factor involved is likely PEDF, as a ... seminiferous tubules. Dihydrotestosterone (DHT) increased PEDF (qPCR) in HTPCs, however PEDF expression in the testis of a ... contribute to the spermatogonial stem cell niche. Hence HTPCs via control of testicular microvessels may contribute to ...
Tópico(s): Testicular diseases and treatments
2015 - Nature Portfolio | Scientific Reports
Luise Landreh, Katrin Spinnler, Kerstin Schubert, Merja R. Häkkinen, Seppo Auriola, Matti Poutanen, Olle Söder, Konstantin Svechnikov, Artur Mayerhofer,
... the differentiation potential of human testicular peritubular cells (HTPCs) and to explore their possible relationship to the ... steroidogenic potential. Methods: Human testicular biopsies, and derived HTPCs, were analyzed by immunohistochemistry, RT-PCR, and Western ... CYP17A1; 3β-hydroxysteroid dehydrogenase) were expressed by the HTPCs. Stimulation with forskolin increased the expression of the ... by the production of pregnenolone and progesterone by HTPCs in vitro. Treatment with platelet-derived growth factor- ...
Tópico(s): Testicular diseases and treatments
2014 - Oxford University Press | The Journal of Clinical Endocrinology & Metabolism
Florian Flenkenthaler, S. Windschüttl, Thomas Fröhlich, Julia Schwarzer, Artur Mayerhofer, Georg J. Arnold,
... wall of seminiferous tubules, human testicular peritubular cells (HTPCs), are considered to contribute to this microenvironment and ... via secreted proteins. Therefore, the secretome of cultured HTPCs from five individual men was analyzed by LC- ... MS. Quantification and comparison to the proteome of HTPC lysates revealed 263 out of 660 identified secretome ... support the hypothesis of a crucial role of HTPCs in the composition of SSC niches in man.
Tópico(s): Xenotransplantation and immune response
2014 - American Chemical Society | Journal of Proteome Research
Müge Molbay, Eylem Özaydın-Goksu, Dijle Kipmen‐Korgun, Ali Ünal, M. Ozekinci, Erhan Cebeci, Emin Maltepe, Emin Türkay Korgun,
... administration of neuroprotective therapies. Human trophoblast progenitor cells (hTPCs) give rise to the placenta and are responsible ... vessels within the uterus. Here, we tested whether hTPCs promoted to differentiate along neural lineages may exhibit ... middle cerebral artery occlusion and, after 24 h, hTPCs were injected systemically via tail vein. Animals were ... staining indicated that infarct volumes were smaller in hTPC treated animals. Visible myelin recovery was observed in the hTPC injected group with Luxol Fast Blue staining. On ...
Tópico(s): MicroRNA in disease regulation
2020 - Taylor & Francis | International Journal of Neuroscience
Weiwei Yang, Yifan Bao, Jiaoyang Hao, Xialin Hu, Ting Xu, Daqiang Yin,
... using existing data on human therapeutic plasma concentrations (HTPCs) and predicted fish plasma concentrations (FPCs). However, studies ... FPCs were in the ranges of 1/1000 HTPC to HTPC. It is the first read-across study of ...
Tópico(s): Pharmacological Effects and Toxicity Studies
2023 - Cell Press | iScience
Arturo Alfaro‐Díaz, Judith Esmeralda Urías‐Silvas, Guadalupe Loarca‐Piña, Marcela Gaytán‐Martínez, Rogelio Prado-Ramírez, Luis Mojica,
... beans were processed through high temperature-pressure cooking (HTPC) (100 and 121 °C for 15 and 30 ... g (pH 3.0–7.0). Nonetheless, all HTPC treatments and the extrusion treatment at 90 °C, ... the raw treatment. The combination of extrusion or HTPC with 5 kDa membrane ultrafiltration process could be ...
Tópico(s): Meat and Animal Product Quality
2020 - Elsevier BV | LWT
Yidan Tang, Lan Ge, Hang Gu, Yun Bai, Yafei Luo, Chengzhan Li, Xinyu Liu,
... 4H-SiC JBS under high temperature power cycling (HTPC) stress were investigated based on thermal structure function, ... analysis and electrothermal simulation. Results show that, the HTPC stress at different temperature conditions (maximum junction temperature ... was that the increased reverse leakage currents with HTPC stress are due to the Schottky barrier lowering ...
Tópico(s): Semiconductor materials and devices
2019 - Elsevier BV | Microelectronics Reliability
Verónica Rey‐Ares, Soledad Paola Rossi, Kim-Gwendolyn Dietrich, Frank‐Michael Köhn, Julia Schwarzer, H. Welter, Mónica Beatriz Frungieri, Artur Mayerhofer,
... testicular samples and isolated human testicular peritubular cells (HTPCs) to explore sites of PG-synthesis and targets. HTPCs express cyclooxygenase 1 (COX1) and secrete PGE2. Receptors ( ... 4) were specifically identified in peritubular cells. In HTPCs PGE2 significantly increased mRNA levels of the contractility ...
Tópico(s): Inflammatory mediators and NSAID effects
2018 - Elsevier BV | Molecular and Cellular Endocrinology
Nguyễn Tiến Dũng, Cise Unluer,
... hydration agent (HA) and high temperature pre-curing (HTPC). The influence of HA and HTPC on hydration, carbonation and microstructural development was investigated ... ATR-FTIR and SEM. Implementation of HA and HTPC increased the rate and degree of hydration. The ...
Tópico(s): CO2 Sequestration and Geologic Interactions
2017 - Elsevier BV | Cement and Concrete Composites
Feng Zuo, Zhenjia Chen, Xiaodi Shi, Ruican Wang, Shuntang Guo,
... The effects of the high-temperature pressure cooking (HTPC) and traditional cooking methods on the yield and ... tofu products were investigated. Results showed that when HTPC was applied, the content of protein particles increased, ... including hardness, chewiness and springiness, was observed. Moreover, HTPC contributes to the change in the composition of ...
Tópico(s): Food composition and properties
2016 - Elsevier BV | Food Chemistry
Luigi Margiotta‐Casaluci, Stewart F. Owen, Rob I. Cumming, Anna de Polo, Matthew J. Winter, Grace H. Panter, Mariann Rand‐Weaver, John P. Sumpter,
... above the range of Human Therapeutic Plasma Concentrations (HTPCs). Fluoxetine and its metabolite, norfluoxetine, were quantified in ... fish were above the upper value of the HTPC range, whereas no effects were observed at plasma concentrations below the HTPCs. In vivo metabolism of fluoxetine in humans and ...
Tópico(s): Aquaculture disease management and microbiota
2014 - Public Library of Science | PLoS ONE
Zhen Shi, Wenping Sun, Zhongtao Wang, Jing Qian, Wei Liu,
... state-of-the-art high temperature proton conductors (HTPCs), and the proton conductivity of such HTPCs is heavily dependent on the dopant. In this ... the electrical conductivity and sinterability of BaCeO3-based HTPC. BaCe0.8Sm(x)Y(0.2-x)O( ...
Tópico(s): Advancements in Solid Oxide Fuel Cells
2014 - American Chemical Society | ACS Applied Materials & Interfaces
High temperature proton conductors (HTPCs) find their applications in steam electrolysis, gas sensing, and most importantly, fuel cells. In this work, proton conductivities and transport ... C), and are much higher than other common HTPCs, such as LaPO4 and LaNbO4. The high conductivity and good sinterability make BaSnO3 a promising HTPC.
Tópico(s): Advanced Condensed Matter Physics
2012 - Elsevier BV | International Journal of Hydrogen Energy