Helena Fernandes, Helena Perés, António Paulo Carvalho,
A standard diet for zebrafish, based on their specific nutritional requirements, is of primary importance to improve experimental outcomes with this model organism and optimize its large-scale production. However, the main basic nutritional requirements of zebrafish are yet to be determined. This study aimed at contributing to fill this gap by evaluating the dietary protein requirement of zebrafish juvenile. Ten isoenergetic fishmeal-based diets with increasing protein levels (15%-60%) were formulated, ...
Tópico(s): Fish biology, ecology, and behavior
2016 - Mary Ann Liebert, Inc. | Zebrafish
Marta Ferreira, Helena Fernandes, Helena Perés, Aires Oliva‐Teles, Isabel Belo, José Manuel Salgado,
Oilseed cakes have potential for new applications as substrates for solid-state fermentation (SSF), to increase their nutritional value by increasing its polyunsaturated fatty acids (PUFAs) or protein content. In this sense, it was performed a screening of oilseed cakes to be used as substrates for the production of PUFAs by Mortierella alpina Peyronel MUM 9412. Of all by-products tested, linseed cake (LSC) was the oilseed cake that achieved the highest production of total PUFAs: 153.09 ± 2.25 mg/g. ...
Tópico(s): Algal biology and biofuel production
2020 - Elsevier BV | LWT
João Costa‐Rodrigues, Anabela C. Fernandes, Maria Helena Fernandes,
Osteosarcoma is usually associated with a disturbed bone metabolism. The aim of this work was to characterize the reciprocal interactions between MG63 osteosarcoma cells and osteoclasts, in a co-culture system. Co-cultures were characterized throughout 21 days for the osteoclastogenic response and the expression of osteoblastic markers. Monocultures of MG63 cells and peripheral blood mononuclear cell (PBMC) and co-cultures of PBMC + human bone marrow cells (hBMC) were also performed. Compared to PBMC ...
Tópico(s): Bone health and treatments
2011 - Wiley | Journal of Cellular Biochemistry
João Costa‐Rodrigues, Anabela C. Fernandes, Maria Helena Fernandes,
Osteoclasts are descended from the CD14(+) monocyte/macrophage lineage, but influence of other haematopoietic cells on osteoclastic commitment of their precursors has remained poorly understood. In this study, osteoclastogenic behaviour of peripheral blood mononuclear cells (PBMC) and their CD14(+) and CD14(-) subpopulations has been accessed, in the absence or presence of M-CSF and RANKL.Cell cultures were characterized for presence of actin rings and vitronectin and calcitonin receptors, TRAP activity ...
Tópico(s): Immune Response and Inflammation
2011 - Wiley | Cell Proliferation
Produção Nacional
Revisado por pares
Bruno F. Fernandes, Luiza Helena Fernandes, Miguel N. Burnier,
Case report: To report the case of a 58-year-old man with blurred vision and metamorphopsia who had an amelanotic choroidal mass in the right eye as the presenting sign of a small cell lung carcinoma. Systemic screening failed to reveal a tumor elsewhere, and the lesion was initially treated as a primary ocular tumor. Discovery of the primary site was made 10 months after the ocular diagnosis, and the patient was then treated with systemic chemotherapy and local radiation therapy. Comments: The ...
Tópico(s): interferon and immune responses
2006 - Elsevier BV | Canadian Journal of Ophthalmology
Amir Zomorodian, Mónica Pereira Garcia, Teresa M. Silva, J.C.S. Fernandes, Maria Helena Fernandes, M.F. Montemor,
In this work a biofunctional composite coating architecture for controlled corrosion activity and enhanced cellular adhesion of AZ31 Mg alloys is proposed. The composite coating consists of a polycaprolactone (PCL) matrix modified with nanohydroxyapatite (HA) applied over a nanometric layer of polyetherimide (PEI). The protective properties of the coating were studied by electrochemical impedance spectroscopy (EIS), a non-disturbing technique, and the coating morphology was investigated by field emission ...
Tópico(s): Orthopaedic implants and arthroplasty
2014 - Elsevier BV | Materials Science and Engineering C
Amir Zomorodian, Mónica Pereira Garcia, Teresa M. Silva, J.C.S. Fernandes, Maria Helena Fernandes, M.F. Montemor,
The high corrosion rate of magnesium alloys is the main drawback to their widespread use, especially in biomedical applications. There is a need for developing new coatings that provide simultaneously corrosion resistance and enhanced biocompatibility. In this work, a composite coating containing polyether imide, with several diethylene triamine and hydroxyapatite contents, was applied on AZ31 magnesium alloys pre-treated with hydrofluoric acid by dip coating. The coated samples were immersed in ...
Tópico(s): Aluminum Alloys Composites Properties
2013 - Elsevier BV | Acta Biomaterialia
Diogo Filipe, Helena Fernandes, Carolina Castro, Helena Perés, Aires Oliva‐Teles, Isabel Belo, José Manuel Salgado,
Abstract Olive pomace is characterized by its low nutritional value and high phenolic content, which hinders its direct use as animal feed, fertilizer, or as a substrate in bioprocesses such as solid‐state fermentation (SSF). A possible strategy for bioprocessing olive pomace by SSF is the mixture of olive mill wastes with other wastes produced in the same region, such as winery wastes. This may improve the production of bioactive compounds like enzymes and antioxidant phenolics. A simplex‐centroid ...
Tópico(s): Enzyme Production and Characterization
2019 - Wiley | Biofuels Bioproducts and Biorefining
A.M.F.R. Pinto, Maria Helena Fernandes, Carlos A. Pinto, Helena Albano, F. Castilho, Paula Teixeira, Peter Gibbs,
This work had as main objectives to characterize two bacteriocins produced by lactic acid bacteria (LAB) previously isolated from non-fermented seafood, in order to evaluate their potential as new food protective agents. The two bacteriocinogenic isolates were identified by Polymerase Chain Reaction (PCR) using genus- and species-specific primers, and confirmed by 16S rDNA sequencing, as Enterococcus faecium and Pediococcus pentosaceus. The antimicrobial spectrum of each strain included several indicator ...
Tópico(s): Identification and Quantification in Food
2008 - Elsevier BV | International Journal of Food Microbiology
Pedro Ferrás Fernandes, Liliana Grenho, Maria Helena Fernandes, J.C. Sampaio-Fernandes, Pedro S. Gomes,
Abstract Statement of problem Microgap and bacterial microleakage at the implant-prosthetic abutment interface are recognized concerns for implant-supported restorations, leading to inflammation of the peri-implant tissues, with deleterious consequences for crestal bone levels. However, little is known regarding the interface established between the implant and the healing abutment or cover screw placed for the osseointegration phase. Purpose The purpose of this in vitro study was to characterize the implant- ...
Tópico(s): Dental materials and restorations
2021 - Elsevier BV | Journal of Prosthetic Dentistry
João Costa‐Rodrigues, Anabela C. Fernandes, M.A. Lopes, Maria Helena Fernandes,
Tópico(s): Bone health and treatments
2011 - Elsevier BV | Acta Biomaterialia
D.S. Morais, Susana Fernandes, Pedro S. Gomes, Maria Helena Fernandes, Paula Sampaio, María Pía Ferraz, José D. Santos, M.A. Lopes, Sooraj Hussain Nandyala,
The aim of this work was to develop a bioactive bone substitute with an effective antibacterial ability based on a cerium (Ce) doped glass-reinforced hydroxyapatite (GR-HA) composite. Developed composites were physicochemically characterized, using x-ray diffraction (XRD) analysis, SEM, energy dispersive x-ray spectroscopy (EDS), and flexural bending strength (FBS) tests. X-ray photoelectron spectroscopy (XPS) analysis was performed to analyze the oxidation state of Ce in the prepared doped glass. The ...
Tópico(s): Dental Implant Techniques and Outcomes
2015 - IOP Publishing | Biomedical Materials
José C. Almeida, András Wacha, Pedro S. Gomes, L.C. Alves, M.H.V. Fernandes, Isabel M. Miranda Salvado, Maria Helena Fernandes,
The increasing interest in the effect of strontium in bone tissue repair has promoted the development of bioactive materials with strontium release capability. According to literature, hybrid materials based on the system PDMS-SiO2 have been considered a plausible alternative as they present a mechanical behavior similar to the one of the human bone. The main purpose of this study was to obtain a biocompatible hybrid material with simultaneous calcium and strontium release capability. A hybrid material, ...
Tópico(s): Bone health and osteoporosis research
2016 - Elsevier BV | Materials Science and Engineering C
Helena Fernandes, José Manuel Salgado, Nicole Martins, Helena Perés, Aires Oliva‐Teles, Isabel Belo,
The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed and unwashed Ulva rigida. SSF of unwashed macroalgae showed higher xylanase (359.8 U/g), cellulase (73.07 U/g) and β-glucosidase (14.9 U/g) activities per dry mass of macroalgae. After SSF, two strategies to carry out EH ...
Tópico(s): Protein Hydrolysis and Bioactive Peptides
2019 - Elsevier BV | Bioresource Technology
Paulina Leite, Daniel Sousa, Helena Fernandes, Marta Ferreira, Ana Rita Costa, Diogo Filipe, Margarida Gonçalves, Helena Perés, Isabel Belo, José Manuel Salgado,
Agricultural, forestry, and food industries produce large amounts of lignocellulosic wastes every year. Land disposal of these residues without proper treatment leads to environmental pollution and negative health effects. The recent advances in valorization of agro-industrial wastes by the production of lignocellulolytic enzymes under solid-state fermentation (SSF) are reviewed. SSF is a promising technology to produce lignocellulolytic enzymes. However, the large-scale feasibility is the main challenge ...
Tópico(s): Enzyme Catalysis and Immobilization
2020 - Elsevier BV | Current Opinion in Green and Sustainable Chemistry
Helena Fernandes, Francisco Javier Moyano, Carolina Castro, José Manuel Salgado, Francisca P. Martínez, María J. Aznar, Nélson Fernandes, Patrícia Ferreira, Margarida Gonçalves, Isabel Belo, Aires Oliva‐Teles, Helena Perés,
Brewer's spent grain (BSG) is the largest by-product originated from the brewery industry with a high potential for producing carbohydrases by solid-state fermentation. This work aimed to test the efficacy of a carbohydrases-rich extract produced from solid-state fermentation of BSG, to enhance the digestibility of a plant-based diet for European seabass (Dicentrarchus labrax). First, BSG was fermented with A. ibericus to obtain an aqueous lyophilized extract (SSF-BSG extract) and incorporated in a ...
Tópico(s): Animal Nutrition and Physiology
2021 - Nature Portfolio | Scientific Reports
Marta Ferreira, José Manuel Salgado, Helena Fernandes, Helena Perés, Isabel Belo,
Seaweeds are valuable feedstocks with the potential to be used as ingredients in aquafeeds. However, their use are still limited, given their recalcitrant polysaccharide structure. To break this structure, a biotechnological approach such as solid-state fermentation (SSF) by filamentous fungi can be used, which simultaneously increases the nutritional value of the biomass. However, SSF has hardly been studied in seaweeds; thus, in this study, five different seaweeds (Gracilaria sp., Porphyra dioica, ...
Tópico(s): Enzyme Production and Characterization
2022 - Multidisciplinary Digital Publishing Institute | Foods
D. Mata, M. Amaral, A.J.S. Fernandes, Bruno Colaço, Adelina Gama, María C. Paiva, Pedro S. Gomes, R.F. Silva, Maria Helena Fernandes,
Diels–Alder functionalization accounts for the improved CNT biological response in terms of biocompatibility and biodegradability profiles.
Tópico(s): Graphene and Nanomaterials Applications
2015 - Royal Society of Chemistry | Nanoscale
Produção Nacional
Revisado por pares
Fernando Mayer Pelicice, Ângelo Antônio Agostinho, Alberto Akama, José Dilermando Andrade Filho, Valter M. Azevedo‐Santos, Marcus Vinícius Moreira Barbosa, Luís Maurício Bini, Marcelo Fulgêncio Guedes de Brito, Carlos Roberto A. Candeiro, Érica Pellegrini Caramaschi, Priscilla Carvalho, Rodrigo Assis de Carvalho, Leandro Castello, Davi Borges das Chagas, Carine C. Chamon, Guarino Rinaldi Colli, Vanessa S. Daga, Murilo S. Dias, José Alexandre Felizola Diniz‐Filho, Philip M. Fearnside, Wagner de Melo Ferreira, Diego Azevedo Zoccal Garcia, Tiago Kütter Krolow, Rodrigo Ferreira Krüger, Edgardo M. Latrubesse, Dilermando P. Lima‐Junior, Solange de Fátima Lólis, Fabyano Álvares Cardoso Lopes, Rafael Loyola, André Lincoln Barroso Magalhães, Adriana Malvásio, Paulo de Marco Júnior, Pedro Ribeiro Martins, Rosana Mazzoni, João Carlos Nabout, Mário Luís Orsi, André Andrian Padial, Hasley Rodrigo Pereira, Thiago Nilton Alves Pereira, Phâmela Bernardes Perônico, Miguel Petrere, Renato Torres Pinheiro, Etiene Fabbrin Pires, Paulo dos Santos Pompeu, Thiago Costa Gonçalves Portelinha, Edson Eyji Sano, Vagner Leonardo Macêdo dos Santos, Paloma Helena Fernandes Shimabukuro, Idelina Gomes da Silva, Lucas Barbosa e Souza, Francisco Leonardo Tejerina‐Garro, Mariana Pires de Campos Telles, Fabrício Barreto Teresa, Sidinei Magela Thomaz, Lívia Helena Tonella, Ludgero Cardoso Galli Vieira, Jean Ricardo Simões Vitule, Jansen Zuanon,
The Tocantins-Araguaia Basin is one of the largest river systems in South America, located entirely within Brazilian territory. In the last decades, capital-concentrating activities such as agribusiness, mining, and hydropower promoted extensive changes in land cover, hydrology, and environmental conditions. These changes are jeopardizing the basin's biodiversity and ecosystem services. Threats are escalating as poor environmental policies continue to be formulated, such as environmentally unsustainable ...
Tópico(s): Hydropower, Displacement, Environmental Impact
2021 - Springer Science+Business Media | Environmental Management
Osteoblast-like cell cultures have been used as in vitro models to study the interactions of bone tissue with biomaterials and their degradation products. This work reviews the effects of AISI 316L stainless steel (SS) corrosion products on the osteoblastic behavior of rat and human bone cell cultures. Results suggest that such products affect, in a dose-dependent manner, the proliferation and differentiation of osteoblastic cells; these effects depend on the developmental stage of the osteoblastic ...
Tópico(s): Bone Tissue Engineering Materials
1999 - SAGE Publishing | Journal of Biomaterials Applications
Simone Morais, Nicolina Dias, João P. Sousa, Maria Helena Fernandes, Graça Simões de Carvalho,
For periods up to 21 days human bone marrow was cultured in control conditions that favor the proliferation and differentiation of osteoblastic cells. The effect of AISI 316L corrosion products and the corresponding major separate metal ions (Fe, Cr, and Ni) were studied in three different phases of the culture period in order to investigate the effects of metal ions in cell populations representative of osteoblastic cells in different stages of differentiation. Toxicity consequences of the presence ...
Tópico(s): Bone Metabolism and Diseases
1999 - Wiley | Journal of Biomedical Materials Research
María Pía Ferraz, Maria Helena Fernandes, Abel Trigo Cabral, José D. Santos, Fernando J. Monteiro,
Tópico(s): Orthopaedic implants and arthroplasty
1999 - Springer Science+Business Media | Journal of Materials Science Materials in Medicine
João Costa‐Rodrigues, Isabel Rocha, Maria Helena Fernandes,
Cigarette smoke is associated to pathological weakening of bone tissue, being considered an important playmaker in conditions such as osteoporosis and periodontal bone loss. In addition, it is also associated with an increased risk of failure in bone regeneration strategies. The present work aimed to characterize the effects of nicotine on human osteoclastogenesis over a hydroxyapatite substrate. Osteoclast precursors were maintained in the absence or presence of the osteoclastogenesis enhancers ...
Tópico(s): Orthopaedic implants and arthroplasty
2017 - Wiley | Journal of Cellular Physiology
Gabriela Ruphuy, Mariana Souto‐Lopes, Diana Paiva, Patrícia Costa, Alı́rio E. Rodrigues, Fernando J. Monteiro, Christiane L. Salgado, Maria Helena Fernandes, José Carlos B. Lopes, Madalena M. Dias, Maria Filomena Barreiro,
Abstract Hybrid scaffolds composed of hydroxyapatite (HAp), in particular in its nanometric form (n‐HAp), and chitosan (CS) are promising materials for non‐load‐bearing bone graft applications. The main constraints of their production concern the successful implementation of the final purification/neutralization and sterilization steps. Often, the used purification strategies can compromise scaffold structural features, and conventional sterilization techniques can result in material's thermal degradation ...
Tópico(s): Dental materials and restorations
2017 - Wiley | Journal of Biomedical Materials Research Part B Applied Biomaterials
Daniel Santos, Dina M. Silva, Pedro S. Gomes, Maria Helena Fernandes, José D. Santos, Vítor Sencadas,
A novel method to process electrospun poly(l-lactic acid) (PLLA) membranes incorporating glass reinforced hydroxyapatite granules (gHA) interspacially between the polymeric fibers is reported, thus increasing the surface area for cellular interactions. gHA granules (≤150 μm) electrospun together with the polymer solution, lead to an average fiber diameter of 550 ± 150 nm for pristine PLLA and 440 ± 170 nm for the composite samples. An increase of the overall porosity was observed, from 79 ± 3% for the PLLA ...
Tópico(s): biodegradable polymer synthesis and properties
2017 - Elsevier BV | Journal of Colloid and Interface Science
Ângela Carvalho, Liliana Cangueiro, V. Oliveira, R. Vilar, Maria Helena Fernandes, Fernando J. Monteiro,
The use of topographic patterns has been a continuously growing area of research for tissue engineering and it is widely accepted that the surface topography of biomaterials can influence and modulate the initial biological response. Ultrafast lasers are extremely powerful tools to machine and pattern the surface of a wide range of biomaterials, however, only few work has been performed on ceramics with the intent of biomedical applications, and the biological characterization of these structured ...
Tópico(s): Laser Applications in Dentistry and Medicine
2017 - Elsevier BV | Applied Surface Science
Produção Nacional
Revisado por pares
Paloma Helena Fernandes Shimabukuro, Andrey José de Andrade, Eunice Aparecida Bianchi Galati,
Phlebotomine sand flies are dipteran insects of medical importance because many species are involved in the transmission of pathogens between human and non-human animals. A total of 530 American species of sand flies is presented in an updated checklist, along with their author(s) and year of publication using the classification by Galati (1995, 2003). Distribution by country is also provided.
Tópico(s): Vector-borne infectious diseases
2017 - Pensoft Publishers | ZooKeys
Produção Nacional
Revisado por pares
Deyse Helena Fernandes da Cunha, Marco Aurélio de Moraes, Miguel Rodolpho Benjamin, Amélia Miyashiro Nunes dos Santos,
RESUMO Objetivo Descrever a qualidade de vida e fatores associados ao menor escore de qualidade de vida de estudantes de medicina. Métodos Estudo transversal com o questionário WHOQOL-100, incluindo alunos do 1º ao 6o ano de medicina da Universidade Federal de São Paulo. Não houve critérios de exclusão. Variáveis numéricas foram comparadas por teste t ou ANOVA e as categóricas, por qui-quadrado. Fatores associados ao menor escore global foram analisados por regressão linear, considerando-se significante ...
Tópico(s): Healthcare professionals’ stress and burnout
2017 - UNIVERSIDADE FEDERAL DO RIO DE JANEIRO | Jornal Brasileiro de Psiquiatria
Produção Nacional
Revisado por pares
José Dilermando Andrade Filho, Ronaldo Guilherme Carvalho Scholte, Alberto Amaral, Paloma Helena Fernandes Shimabukuro, Omar S Carvalho, Roberta Lima Caldeira,
Leishmaniases are serious diseases caused by trypanosomatid protozoans of the genus Leishmania transmitted by the bite of phlebotomine sand flies. We analyzed records pertaining to Lutzomyia longipalpis (Lutz and Neiva, 1912) and Lutzomyia cruzi (Mangabeira, 1938) in Brazil from the following sources: the collection of phlebotomine sand flies of the Centro de Pesquisas René Rachou/Fiocruz (FIOCRUZ-COLFLEB), the "SpeciesLink" (CRIA) database, from systematic surveys of scientific articles and gray literature ( ...
Tópico(s): Mosquito-borne diseases and control
2017 - Oxford University Press | Journal of Medical Entomology
Daniel Santos, Cristina Correia, Dina M. Silva, Pedro S. Gomes, Maria Helena Fernandes, José D. Santos, Vítor Sencadas,
Tissue engineering is constantly evolving towards novel materials that mimic the properties of the replaced injured tissue or organ. A hybrid electrospun membrane of electroactive poly(l-acid lactic) (PLLA) polymer with glass reinforced hydroxyapatite (Bonelike®) microparticles placed among the polymer fibres in a morphology like "islands in the sea" was processed. The incorporation of 60 to 80wt% Bonelike® bone grafts granules with ≤150μm into the polymer solution lead to an amorphous polymeric fibre ...
Tópico(s): biodegradable polymer synthesis and properties
2017 - Elsevier BV | Materials Science and Engineering C