Rocio Diaz Escarcega, Abhijeet A. Patil, Jose F. Moruno-Manchon, Akihiko Urayama, Sean P. Marrelli, Nayun Kim, David Monchaud, Louise D. McCullough, Andrey S. Tsvetkov,
... by G4 stabilization. Pirh2 downregulated DDX21, an RNA helicase that unfolds G4-RNA and R-loops. Finally, ... by G4 stabilization. Pirh2 downregulated DDX21, an RNA helicase that unfolds G4-RNA and R-loops. Finally, ... are functionally modulated in cells by chaperones and helicases (2Meier-Stephenson V. G4-quadruplex-binding proteins: review ... Scholar, 3Sauer M. Paeschke K. G-quadruplex unwinding helicases and their function in vivo.Biochem. Soc. Trans. ... by G4-DNA-binding proteins and G4-DNA helicases (3Sauer M. Paeschke K. G-quadruplex unwinding helicases ...
Tópico(s): RNA Interference and Gene Delivery
2023 - Elsevier BV | Journal of Biological Chemistry
Directory of Open Access Books (DOAB)
Shizhuo Yang, Lacey Winstone, Sohaumn Mondal, Yuliang Wu,
... processes, including transcription, DNA repair, and telomere maintenance. Helicases are enzymes that use an ATP-driven motor ... DNA, dsRNA, or RNA–DNA hybrids. Additionally, certain helicases have strand-annealing activity. Thus, helicases possess unique positions for R-loop biogenesis: they ... DNA hybrids and resolve R-loops. Indeed, numerous helicases such as senataxin (SETX), Aquarius (AQR), WRN, BLM, ... are reported to resolve R-loops; while other helicases, such as Cas3 and UPF1, are reported to stimulate R-loop formation. Moreover, helicases like DDX1, DDX17, and DHX9 have been identified ...
Tópico(s): Chromosomal and Genetic Variations
2023 - Elsevier BV | Journal of Biological Chemistry
Monalisha Das Mohapatra, Sowmya Poosapati, Ranjan Kumar Sahoo, Durga Madhab Swain,
... improve stress tolerance and high-yielding crop plants. Helicases are such molecular proteins that present as one ... stress-tolerant trait is required for food security. Helicases are involved in the process of nucleic acid ... stress tolerance in plants. Both DNA and RNA helicase highly influences the plant's defense mechanism that ... plants from pathogen infection and other stress factors Helicase is the molecular conserved enzyme which classified into six superfamilies from SF1 to SF6. DNA helicase is a protein and acts as a molecular ...
Tópico(s): Plant Stress Responses and Tolerance
2023 - Elsevier BV | Plant Stress
Jillian Chase, James M. Berger, David Jeruzalmi,
... assembly of essential factors, including the replicative hexameric helicase complex, to replication origins. A crucial step during ... replication is loading of hexameric, ring-shaped replicative helicases onto DNA. In bacteria, the DnaB family of replicative helicases comprise six identical subunits, which collectively create a ... DNA synthesis. Recent structure determinations of two bacterial helicase loaders bound to the same DnaB helicase offer an opportunity to extract fundamental principles associated ...
Tópico(s): Bacteriophages and microbial interactions
2022 - Elsevier BV | Trends in Biochemical Sciences
Michael M. Soniat, Giaochau Nguyen, Hung‐Che Kuo, Ilya J. Finkelstein,
... resectosome, a multienzyme complex that includes bloom syndrome helicase (BLM), DNA2 or exonuclease 1 nucleases, and additional ... efficiently and how TOP3A–RMI1/2 regulates the helicase activity of BLM to promote efficient DNA repair. ... resectosome, a multienzyme complex that includes bloom syndrome helicase (BLM), DNA2 or exonuclease 1 nucleases, and additional ... efficiently and how TOP3A–RMI1/2 regulates the helicase activity of BLM to promote efficient DNA repair. ... resectosome is a multienzyme complex composed of a helicase, a nuclease, and regulatory proteins. In humans, resection ...
Tópico(s): DNA and Nucleic Acid Chemistry
2022 - Elsevier BV | Journal of Biological Chemistry
Eleni Pitsillou, Julia Liang, Andrew Hung, Tom C. Karagiannis,
... proteins are of interest and this includes the helicase (nsp13). In this study, an initial screen of ... structures of the SARS-CoV and MERS-CoV helicases, as well as the human RECQ-like DNA helicase, DHX9 helicase, PcrA helicase, hepatitis C NS3 helicase, and mouse Dna2 nuclease-helicase were used for comparison. As expected, sequence and ... potential lead compounds for the SARS-CoV-2 helicase. Molecular dynamics simulations were performed with the leads bound to the SARS-CoV-2 helicase for 200 ns in triplicate, with favourable binding ...
Tópico(s): Computational Drug Discovery Methods
2022 - Elsevier BV | Journal of Molecular Graphics and Modelling
Mohammad Sadic, William M. Schneider, Olga Katsara, Gisselle N. Medina, Ashley Fisher, Aishwarya Mogulothu, Yingpu Yu, Meigang Gu, Teresa de los Santos, Robert J. Schneider, Meike Dittmann,
... protein synthesis. Here, we describe an interferon-stimulated helicase, DDX60, that decreases translation from viral internal ribosome ... to 3′ direction. During scanning, eIF4G-bound RNA helicase eIF4A and activator protein eIF4B unwind RNA secondary ... Here, we describe the ISG DExD/H-box helicase 60 (DDX60), an RNA helicase that can inhibit viral type II IRES-driven ... that N- and C-terminal regions alongside predicted helicase and ATP binding motifs in DDX60 are important ... Schematic of DDX60 protein with putative functional domains. Helicase ATP binding type I domain (amino acids 785– ...
Tópico(s): RNA and protein synthesis mechanisms
2022 - Springer Nature | EMBO Reports
Zeynep Tarcan, Divyasree Poovathumkadavil, Aggeliki Skagia, Agnieszka Gambus,
... this process have emerged, revealing that the replicative helicase, at the heart of the replisome, is polyubiquitylated ... ligases have now been shown to ubiquitylate the helicase under different conditions: Cul2Lrr1 and TRAIP. Here, using ... this process have emerged, revealing that the replicative helicase, at the heart of the replisome, is polyubiquitylated ... ligases have now been shown to ubiquitylate the helicase under different conditions: Cul2Lrr1 and TRAIP. Here, using ... origins, the positions within the genome where replicative helicases become activated and start unwinding DNA. These then ...
Tópico(s): Microtubule and mitosis dynamics
2022 - Elsevier BV | Journal of Biological Chemistry
Jinru Xie, Ming Wen, Jiao Zhang, Zheng Wang, Meng Wang, Yanfang Qiu, Wenchao Zhao, Fang Zhu, Mianfeng Yao, Zhuoxian Rong, Wenfeng Hu, Qian Pei, Xiaoxiang Sun, Jinchen Li, Zhiyong Mao, Lun‐Quan Sun, Rong Tan,
DEAD-box RNA helicases belong to a large group of RNA-processing factors and play vital roles unwinding RNA helices and in ribosomal RNA biogenesis. Emerging evidence indicates that RNA helicases are associated with genome stability, yet the mechanisms ... study, we performed a comprehensive analysis of RNA helicases using multiplatform proteogenomic databases. More than 50% (28/49) of detected RNA helicases were highly expressed in multiple tumor tissues, and ... Analysis of repair dynamics revealed that these RNA helicases are engaged in an extensively broad range of ...
Tópico(s): DNA Repair Mechanisms
2022 - American Association for Cancer Research | Cancer Research
Binyam Belachew, Jun Gao, Alicia K. Byrd, Kevin D. Raney,
... of liver-related diseases and hepatocellular carcinoma. The helicase domain of one of the nonstructural proteins of ... imply a novel biological role of the viral helicase in replication. Hepatitis C virus (HCV) is a ... of liver-related diseases and hepatocellular carcinoma. The helicase domain of one of the nonstructural proteins of ... imply a novel biological role of the viral helicase in replication. Infection with Hepatitis C Virus (HCV) ... protease domain at the N-terminus and a helicase domain at the C-terminus (1Manns M.P. ...
Tópico(s): RNA and protein synthesis mechanisms
2022 - Elsevier BV | Journal of Biological Chemistry
Brandon Schweibenz, Swapnil C. Devarkar, Mihai Solotchi, Candice Craig, Jie Zheng, Bruce D. Pascal, Samantha Gokhale, Ping Xie, Patrick R. Griffin, Smita S. Patel,
... 2022Open Access Transparent process The intrinsically disordered CARDs-Helicase linker in RIG-I is a molecular gate ... domain (CTD), but the RNA must engage the helicase domain to release the signaling CARD (Caspase Activation ... from their autoinhibitory CARD2:Hel2i interactions. Because the helicase itself lacks RNA specificity, mechanisms to proofread RNAs entering the helicase domain must exist. Although such mechanisms would be ... mechanism through which RIG-I ensures that the helicase engages RNAs explicitly recognized by the CTD. A ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2022 - Springer Nature | The EMBO Journal
... among RNA viruses. The DDX DEAD box RNA helicase is a multifunctional protein involved in all aspects of RNA metabolism. Therefore, host RNA helicases may regulate and maintain such a large viral ... the potential role of several host cellular RNA helicases in SARS-CoV-2 infection. Notably, DDX21 knockdown ... SARS-CoV-2 infection. In addition, MOV10 RNA helicase also suppressed the SARS-CoV-2 infection. In contrast, DDX1, DDX5, and DDX6 RNA helicases were required for SARS-CoV-2 replication. Indeed, ... P-body formation of DDX6 and MOV10 RNA helicases as well as XRN1 exonuclease, while the viral ...
Tópico(s): RNA regulation and disease
2022 - American Society for Microbiology | Journal of Virology
Aditya Mojumdar, Nancy Adam, Jennifer A. Cobb,
... inhibition of 5′-resection mediated by the nuclease/helicase complex Dna2–Sgs1. Here, we show that Nej1 ... inhibition of 5′-resection mediated by the nuclease/helicase complex Dna2–Sgs1. Here, we show that Nej1 ... Nej1 inhibits resection mediated by Dna2-Sgs1 nuclease-helicase at DNA double strand breaks.J. Biol. Chem. ... E.Y. Lee S.E. Ira G. Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA ... Nej1 inhibits resection mediated by Dna2-Sgs1 nuclease-helicase at DNA double strand breaks.J. Biol. Chem. ...
Tópico(s): Microtubule and mitosis dynamics
2022 - Elsevier BV | Journal of Biological Chemistry
DEAD-box RNA helicases, the largest family of superfamily 2 helicases, are a profoundly conserved family of RNA-binding proteins, containing a distinctive ... duplexes, which set up these proteins as RNA helicases, DEAD-box proteins have been found to additionally ... complexes. There is accumulating evidence that DEAD-box helicases play functions in the recognition of foreign nucleic ... to assist replication. The capability of DEAD-box helicases to sense RNA in a sequence-independent way, ... viral infections in numerous manners. Undoubtedly, DEAD-box helicases have been demonstrated to contribute to intracellular immune ...
Tópico(s): interferon and immune responses
2021 - Elsevier BV | Virus Research
Pascal Donsbach, Dagmar Klostermeier,
RNA helicases are a ubiquitous class of enzymes involved in virtually all processes of RNA metabolism, from transcription, mRNA ... RNA duplexes is their hallmark reaction, not all helicases catalyze unwinding in vitro, and some in vivo functions do not depend on duplex unwinding. RNA helicases are divided into different families that share a common helicase core with a set of helicase signature motives. The core provides the active site ... regulatory mechanisms that modulate the activities of the helicase core. Case studies on selected helicases with functions ...
Tópico(s): RNA modifications and cancer
2021 - De Gruyter | Biological Chemistry
Rizwan Ullah, Jia Li, Puxian Fang, Shaobo Xiao, Liurong Fang,
DEAD/H-box RNA helicases make the prominent family of helicases super family-2 which take part in almost all RNA-related processes, from initiation ... in recent years a certain number of these helicases are reported to play important roles in anti- ... endosomal TLRs, and cytosolic DNA receptors, many RNA helicases including DDX3, DHX9, DDX6, DDX41, DHX33, DDX60, DHX36 ... viral nucleic acid sensors or co-sensors. These helicases mostly follow RLHs-MAVS and STING mediated signaling ... many studies indicated that several DEAD/H-box helicases such as DDX1, DDX3, DDX6, DDX24, and DHX9 ...
Tópico(s): RNA Research and Splicing
2021 - Elsevier BV | Virus Research
Richard R. Spinks, Lisanne M. Spenkelink, Nicholas E. Dixon, Antoine M. van Oijen,
Helicases are molecular motors that translocate along single-stranded DNA and unwind duplex DNA. They rely on ... from nucleotide hydrolysis to drive their translocation. Specialized helicases play a critically important role in DNA replication ... the front of the replication fork. The replicative helicases of the model systems bacteriophages T4 and T7, ... states in the unwinding process and the dynamic helicase interactions within the replisome. Here, we describe single- ... details that these methods have revealed about replicative helicases. Applying methods such as FRET and optical and ...
Tópico(s): DNA and Nucleic Acid Chemistry
2021 - Frontiers Media | Frontiers in Molecular Biosciences
Ann Schirin Mirsanaye, Dimitris Typas, Niels Mailand,
... can be directly bypassed by the replicative CMG helicase and those requiring prior processing (e.g., interstrand ... of the replicative CDC45–MCM2–7–GINS (CMG) helicase that translocates on the leading strand to unwind ... E. et al.The mechanism of eukaryotic CMG helicase activation.Nature. 2018; 555: 265-268Crossref PubMed Scopus ( ... I. et al.Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.Mol. ... phase, the active replisome, consisting of the CMG helicase, replicative DNA polymerases, the replication factor C (RFC)- ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2021 - Elsevier BV | Trends in Cell Biology
Yisui Xia, Ryo Fujisawa, Tom Deegan, Remi Sonneville, Karim Labib,
... CUL-2LRR-1 drives ubiquitylation of the CMG helicase, leading to replisome disassembly by the p97/CDC- ... for CUL-2LRR-1 recruitment and efficient CMG helicase ubiquitylation. Aided by TIMELESS-TIPIN, CUL-2LRR-1 ... Caenorhabditis elegans TIM-1_TIPN-1 stimulates CMG helicase ubiquitylation by the CUL-2_LRR-1 ligase ... of CUL-2_LRR-1 with the CMG helicase. The TIM-1_TIPN-1 complex stimulates CMG ... S-phase, the replisome assembles around the CMG helicase at nascent DNA replication forks (CMG is named ... budding yeast and metazoa indicates that the CMG helicase is ubiquitylated on its MCM7 subunit during termination ( ...
Tópico(s): Advanced Data Storage Technologies
2021 - Springer Nature | The EMBO Journal
David R. McKinzey, Shivasankari Gomathinayagam, Wezley C. Griffin, Kathleen N. Klinzing, Elizabeth P. Jeffries, Aleksandar Rajkovic, Michael A. Trakselis,
... BRCv) similar to that found in other HR helicases is necessary for localization to sites of MMC ... BRCv) similar to that found in other HR helicases is necessary for localization to sites of MMC ... intermediates and are hallmarks of disease. Various DNA helicases contribute to several steps in the recombination pathways ... S.F. The regulation of homologous recombination by helicases.Genes (Basel). 2020; 11: 498Crossref Scopus (10) Google ... are recent additions to the roster of DNA helicases involved in HR (6Griffin W.C. Trakselis M. ...
Tópico(s): PARP inhibition in cancer therapy
2021 - Elsevier BV | Journal of Biological Chemistry
Kelly E. Du Pont, Martin McCullagh, Brian J. Geiss,
... flavivirus survival. One of the viral proteins, NS3 helicase, is critical for unwinding the double stranded RNA intermediate during flaviviral replication. The helicase performs the unwinding of the viral RNA intermediate structure in an ATP‐dependent manner. NS3 helicase is a member of the Viral/DEAH‐like subfamily of the superfamily 2 helicase containing eight highly conserved structural motifs (I, Ia, ... structural motifs are not well understood for NS3 helicase function, but comparison of NS3 with other superfamily ...
Tópico(s): Insect symbiosis and bacterial influences
2021 - Wiley | Wiley Interdisciplinary Reviews - RNA
Shibo Li, Hailong Wang, Sanaa E. Jehi, Jun Li, Shuo Liu, Zi Wang, Lan N. Truong, Takuya Chiba, Zefeng Wang, Xiaohua Wu,
Article20 January 2021Open Access Source DataTransparent process PIF1 helicase promotes break-induced replication in mammalian cells Shibo ... and demonstrate an important role of human PIF1 helicase in promoting BIR. We show that at endonuclease ... to one end of donor sequence. Here, the helicase PIF1 is found to play a critical BIR ... in mammalian cells. Loss of PIF1 or its helicase activity reduces BIR frequency at induced double-strand ... non-essential subunit of Polδ in yeast, and helicase Pif1 (Lydeard et al, 2007; Deem et al, ...
Tópico(s): Genetics, Aging, and Longevity in Model Organisms
2021 - Springer Nature | The EMBO Journal
Nicolette P. Brown, Ashley M. Vergara, Alisha B. Whelan, Paolo Guerra, Timothy A. Bolger,
... frequent mutations in DDX3X, a DEAD-box RNA helicase primarily implicated in translation. Forty-two different sites ... frequent mutations in DDX3X, a DEAD-box RNA helicase primarily implicated in translation. Forty-two different sites ... these studies was frequent mutations in the RNA helicase DDX3X, which had not previously been linked to ... hand, the mutations were spread throughout the central “helicase domains” of DDX3X, thus complicating any prediction of ... members of the DEAD-box family of RNA helicases, which have critical roles in many facets of ...
Tópico(s): RNA and protein synthesis mechanisms
2021 - Elsevier BV | Journal of Biological Chemistry
Gaetana Sessa, Belén Gómez‐González, Sónia Silva, Carmen Pérez‐Calero, Romane Beaurepere, Sónia Barroso, Sylvain Martineau, Charlotte Martin, Åsa Ehlén, Juan S. Martinez, Bérangère Lombard, Damarys Loew, Stéphan Vagner, Andrés Aguilera, Aura Carreira,
... BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair‡ Gaetana ... from ‘BRCA2 promotes R-loop resolution by DDX5 helicase at DNA breaks to facilitate their repair by ... understood. Here we identified the DEAD-box RNA helicase DDX5 as a BRCA2-interacting protein. DDX5 associates ... the DNA-RNA hybrid-unwinding activity of DDX5 helicase. An impaired BRCA2-DDX5 interaction, as observed in ... in their suppression has remained unclear. Here, RNA helicase DDX5 is found to interact with BRCA2 and ...
Tópico(s): Chromosomal and Genetic Variations
2021 - Springer Nature | The EMBO Journal
DEAD-box helicases are a large family of conserved RNA-binding proteins that belong to the broader group of cellular DExD/H helicases. Members of the DEAD-box helicase family have roles throughout cellular RNA metabolism from ... Moreover, there is emerging evidence that cellular RNA helicases, including DEAD-box helicases, play roles in the recognition of foreign nucleic ... to facilitate replication. The ability of DEAD-box helicases to recognize RNA in a sequence-independent manner, ... viral infections in multiple ways. Indeed, DEAD-box helicases have been shown to contribute to intracellular immune ...
Tópico(s): RNA modifications and cancer
2020 - Multidisciplinary Digital Publishing Institute | Viruses
... replication. Interestingly, cellular ATP-dependent DEAD-box RNA helicases could promote or inhibit the replication of Tomato ... unknown what features make a particular DEAD-box helicase either pro-viral or antiviral. In this work, ... of the antiviral DDX17-like RH30 DEAD-box helicase by converting it to a pro-viral helicase. We also turned the pro-viral DDX3-like RH20 helicase into an antiviral helicase through deletion of a unique N-terminal domain. ... absence of the N-terminal domain, the core helicase domain becomes unhinged, showing altered specificity in unwinding ...
Tópico(s): CRISPR and Genetic Engineering
2020 - Public Library of Science | PLoS Pathogens
Aaron C. Mason, Susan R. Wente,
... a conserved, essential regulator of DEAD-box RNA helicases, with critical roles defined in mRNA export, translation ... a conserved, essential regulator of DEAD-box RNA helicases, with critical roles defined in mRNA export, translation ... Bohnsack M.T. Unravelling the mechanisms of RNA helicase regulation.Trends Biochem. Sci. 2018; 43 (29486979): 237- ... for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies.Mol. Biol. Cell. ... The Ded1/DDX3 subfamily of DEAD-box RNA helicases.Crit. Rev. Biochem. Mol. Biol. 2014; 49 (25039764): ...
Tópico(s): Viral Infections and Immunology Research
2020 - Elsevier BV | Journal of Biological Chemistry
Sarah E. Fritz, Soumya Ranganathan, Clara D. Wang, J. Robert Hogg,
... mediated decay (NMD) by preventing the UPF1 RNA helicase from associating with potential decay targets. Here, by analyzing in vitro helicase activity, dissociation of UPF1 from purified mRNPs, and ... NMD substrates, presenting a new model for RNA helicase regulation and target selection in the NMD pathway. ... mediated decay (NMD) by preventing the UPF1 RNA helicase from associating with potential decay targets. Here, by analyzing in vitro helicase activity, dissociation of UPF1 from purified mRNPs, and ...
Tópico(s): RNA modifications and cancer
2020 - Elsevier BV | Journal of Biological Chemistry