Craig Fenwick, Priscilla Turelli, Dongchun Ni, Laurent Perez, Kelvin Lau, Cécile Hérate, Romain Marlin, Erica Lana, Céline Pellaton, Charlène Raclot, Line Esteves-Leuenberger, Jérémy Campos, Alex Farina, Flurin Fiscalini, Nathalie Dereuddre‐Bosquet, Francis Relouzat, Rana Abdelnabi, Caroline S. Foo, Johan Neyts, Pieter Leyssen, Yves Lévy, Florence Pojer, Henning Stahlberg, R. Legrand, Didier Trono, Giuseppe Pantaleo,
Abstract The SARS-CoV-2 Omicron variant has very high levels of transmission, is resistant to neutralization by authorized therapeutic human monoclonal antibodies (mAb) and is less sensitive to vaccine-mediated immunity. To provide additional therapies against Omicron, we isolated a mAb named P2G3 from a previously infected vaccinated donor and showed that it has picomolar-range neutralizing activity against Omicron BA.1, BA.1.1, BA.2 and all other variants tested. We solved the structure of P2G3 ...
Tópico(s): Bacteriophages and microbial interactions
2022 - Nature Portfolio | Nature Microbiology
Nerea Zabaleta, Wenlong Dai, Urja Bhatt, Cécile Hérate, Pauline Maisonnasse, Jessica A. Chichester, Julio Sanmiguel, Reynette Estelien, Kristofer T. Michalson, Cheikh Tacko Diop, Dawid Maciorowski, Nathalie Dereuddre‐Bosquet, Mariangela Cavarelli, Anne-Sophie Gallouët, Thibaut Naninck, Nidhal Kahlaoui, Julien Lemaître, Wenbin Qi, Elissa M. Hudspeth, Allison Cucalon, Cecilia Dyer, M. Betina Pampena, James J. Knox, Regina C. LaRocque, Richelle C. Charles, Dan Li, Maya Kim, A. Sheridan, Nadia Storm, Rebecca I. Johnson, Jared Feldman, Blake M. Hauser, Vanessa Contreras, Romain Marlin, Raphaël Ho Tsong Fang, Catherine Chapon, Sylvie van der Werf, Eric Zinn, Aisling Ryan, Dione Kobayashi, Ruchi Chauhan, Marion McGlynn, Edward T. Ryan, Aaron G. Schmidt, Brian M. Price, Anna N. Honko, Anthony Griffiths, Sam Yaghmour, R. Anthony Vere Hodge, Michael R. Betts, Mason W. Freeman, James M. Wilson, Roger Le Grand, Luk H. Vandenberghe,
The SARS-CoV-2 pandemic has affected more than 185 million people worldwide resulting in over 4 million deaths. To contain the pandemic, there is a continued need for safe vaccines that provide durable protection at low and scalable doses and can be deployed easily. Here, AAVCOVID-1, an adeno-associated viral (AAV), spike-gene-based vaccine candidate demonstrates potent immunogenicity in mouse and non-human primates following a single injection and confers complete protection from SARS-CoV-2 challenge ...
Tópico(s): CAR-T cell therapy research
2021 - Cell Press | Cell Host & Microbe
Ross W. Cheloha, Thibault J. Harmand, Charlotte Wijne, Thomas Schwartz, Hidde L. Ploegh,
... protein of the Newcastle disease virus (40Matz J. Hérate C. Bouchet J. Dusetti N. Gayet O. Baty ...
Tópico(s): Glycosylation and Glycoproteins Research
2020 - Elsevier BV | Journal of Biological Chemistry
Michelle Ricoul, Tamizh Selvan Gnanasekaran, Patricia Brochard, Cécile Hérate, Laure Sabatier,
Many toxic agents can cause DNA double strand breaks (DSBs), which are in most cases quickly repaired by the cellular machinery. Using ionising radiation, we explored the kinetics of DNA lesion signaling and structural chromosome aberration formation at the intra- and inter-chromosomal level. Using a novel approach, the classic Premature Chromosome Condensation (PCC) was combined with γ-H2AX immunofluorescence staining in order to unravel the kinetics of DNA damage signalisation and chromosome repair. ...
Tópico(s): Carcinogens and Genotoxicity Assessment
2019 - Multidisciplinary Digital Publishing Institute | Cancers
Cécile Hérate, Laure Sabatier,
In the absence of physical data, biodosimetry tools are required for fast dose and risk assessment in the event of radiological or nuclear mass accidents or attacks to triage exposed humans and take immediate medical countermeasures. Biodosimetry tools have mostly been developed for retrospective dose assessment and the follow-up of victims of irradiation. Among them, cytogenetics analyses, to reveal chromosome damage, are the most developed and allow the determination of doses from blood samples ...
Tópico(s): Radiation Effects and Dosimetry
2019 - Elsevier BV | Mutation Research/Reviews in Mutation Research
Damien Roos‐Weil, Camille Decaudin, Marine Armand, Véronique Della-Valle, M’Boyba Diop, Hussein Ghamlouch, Virginie Ropars, Cécile Hérate, Diane Lara, Éric Durot, Rima Haddad, Elena Mylonas, Frédérik Damm, Françoise Pflumio, Bilyana Stoilova, Marlen Metzner, Olivier Elemento, Philippe Dessen, Valérie Camara‐Clayette, François-Loı̈c Cosset, Els Verhoeyen, Véronique Leblond, Vincent Ribrag, Pascale Cornillet‐Lefèbvre, Philippe Rameau, Nabih Azar, Frédéric Charlotte, Pierre Morel, Jean‐Baptiste Charbonnier, Paresh Vyas, Thomas Mercher, Saïd Aoufouchi, Nathalie Droin, Christel Guillouf, Florence Nguyen‐Khac, Olivier Bernard,
The ETS-domain transcription factors divide into subfamilies based on protein similarities, DNA-binding sequences, and interaction with cofactors. They are regulated by extracellular clues and contribute to cellular processes, including proliferation and transformation. ETS genes are targeted through genomic rearrangements in oncogenesis. The PU.1/SPI1 gene is inactivated by point mutations in human myeloid malignancies. We identified a recurrent somatic mutation (Q226E) in PU.1/SPI1 in Waldenström ...
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2019 - American Association for Cancer Research | Cancer Discovery
Cécile Hérate, Ghania Ramdani, Nancy J. Grant, Sabrina Marion, Stéphane Gasman, Florence Niedergang, Serge Bénichou, Jérôme Bouchet,
Phospholipid Scramblase 1 (PLSCR1) was initially characterized as a type II transmembrane protein involved in bilayer movements of phospholipids across the plasma membrane leading to the cell surface exposure of phosphatidylserine, but other cellular functions have been ascribed to this protein in signaling processes and in the nucleus. In the present study, expression and functions of PLSCR1 were explored in specialized phagocytic cells of the monocyte/macrophage lineage. The expression of PLSCR1 ...
Tópico(s): Cellular transport and secretion
2016 - Public Library of Science | PLoS ONE
Julie Matz, Cécile Hérate, Jérôme Bouchet, Nelson Dusetti, Odile Gayet, Daniel Baty, Serge Bénichou, Patrick Chames,
The targeting of HIV-1 using antibodies is of high interest as molecular tools to better understand the biology of the virus or as a first step toward the design of new inhibitors targeting critical viral intracellular proteins. Small and highly stable llama-derived single-domain antibodies can often be functionally expressed as intracellular antibodies in the cytoplasm of eukaryotic cells. Using a selection method based on the Sos Recruitment System, a cytoplasmic yeast two-hybrid approach, we ...
Tópico(s): T-cell and B-cell Immunology
2014 - Public Library of Science | PLoS ONE
Eric C. Logue, Nicolin Bloch, Erica Dhuey, Ruonan Zhang, Ping Cao, Cécile Hérate, Lise Chauveau, Stevan R. Hubbard, Nathaniel R. Landau,
APOBEC3A (A3A), one of the seven-member APOBEC3 family of cytidine deaminases, lacks strong antiviral activity against lentiviruses but is a potent inhibitor of adeno-associated virus and endogenous retroelements. In this report, we characterize the biochemical properties of mammalian cell-produced and catalytically active E. coli-produced A3A. The enzyme binds to single-stranded DNA with a Kd of 150 nM and forms dimeric and monomeric fractions. A3A, unlike APOBEC3G (A3G), deaminates DNA substrates ...
Tópico(s): Bacteriophages and microbial interactions
2014 - Public Library of Science | PLoS ONE
Jérôme Bouchet, Cécile Hérate, Carolin A. Guenzel, Christel Vérollet, Annika Järviluoma, Julie Mazzolini, Salomeh Rafie, Patrick Chames, Daniel Baty, Kalle Saksela, Florence Niedergang, Isabelle Maridonneau‐Parini, Serge Bénichou,
HIV-1 Nef is essential for AIDS pathogenesis, but this viral protein is not targeted by antiviral strategies. The functions of Nef are largely related to perturbations of intracellular trafficking and signaling pathways through leucine-based and polyproline motifs that are required for interactions with clathrin-associated adaptor protein complexes and SH3 domain-containing proteins, such as the phagocyte-specific kinase Hck. We previously described a single-domain antibody (sdAb) targeting Nef and ...
Tópico(s): Immune Cell Function and Interaction
2012 - American Society for Microbiology | Journal of Virology
Carolin A. Guenzel, Cécile Hérate, Erwann Le Rouzic, Priscilla Maidou-Peindara, Holly A. Sadler, Marie-Christine Rouyez, Louis M. Mansky, Serge Bénichou,
ABSTRACT The HIV-1 Vpr protein participates in the early steps of the virus life cycle by influencing the accuracy of reverse transcription. This role of Vpr was related to the recruitment of the nuclear form of the uracil DNA glycosylase (UNG2) enzyme into virus particles, but several conflicting findings have been reported regarding the role of UNG2 encapsidation on viral infectivity. Here, we report that the catalytic activity of UNG2 was not required for influencing HIV-1 mutation, and this function ...
Tópico(s): Herpesvirus Infections and Treatments
2011 - American Society for Microbiology | Journal of Virology
Erik S.G. Stroes, Melchior C. Nierman, J. J. M. Meulenberg, Remco Franssen, Jaap Twisk, C. P. Henny, Mario Maas, Aeilko H. Zwinderman, Colin J.D. Ross, Eleonora Aronica, Katherine A. High, Marcel M. Levi, Michael R. Hayden, John J.P. Kastelein, Jan Albert Kuivenhoven,
... Dec-2021. Zabaleta N, Dai W, Bhatt U, Hérate C, Maisonnasse P, Chichester J, Sanmiguel J, Estelien ...
Tópico(s): Systemic Lupus Erythematosus Research
2008 - Lippincott Williams & Wilkins | Arteriosclerosis Thrombosis and Vascular Biology
Cécile Hérate, Clarisse Vigne, Carolin A. Guenzel, Marie Lambelé, Marie-Christine Rouyez, Serge Bénichou,
Through incorporation into virus particles, the HIV-1 Vpr protein participates in the early steps of the virus life cycle by influencing the reverse transcription process. We previously showed that this positive impact on reverse transcription was related to Vpr binding to the uracil DNA glycosylase 2 enzyme (UNG2), leading to enhancement of virus infectivity in established CD4-positive cell lines via a nonenzymatic mechanism.
Tópico(s): DNA Repair Mechanisms
2016 - BioMed Central | Retrovirology
Sarah Temmam, Xavier Montagutelli, Cécile Hérate, Flora Donati, Béatrice Regnault, Mikaël Attia, Eduard Baquero, Delphine Chrétien, Laurine Conquet, Grégory Jouvion, Juliana Pipoli da Fonseca, Thomas Cokelaer, Faustine Amara, Francis Relouzat, Thibaut Naninck, Julien Lemaître, Nathalie Derreudre‐Bosquet, Quentin Pascal, Massimiliano Bonomi, Thomas Bigot, Sandie Munier, F.A. Rey, Roger Le Grand, Sylvie van der Werf, Marc Éloit,
Abstract Bat sarbecovirus BANAL‐236 is highly related to SARS‐CoV‐2 and infects human cells, albeit lacking the furin cleavage site in its spike protein. BANAL‐236 replicates efficiently and pauci‐symptomatically in humanized mice and in macaques, where its tropism is enteric, strongly differing from that of SARS‐CoV‐2. BANAL‐236 infection leads to protection against superinfection by a virulent strain. We find no evidence of antibodies recognizing bat sarbecoviruses in populations in close contact ...
Tópico(s): Viral Infections and Outbreaks Research
2023 - Springer Nature | EMBO Reports
Cécile Hérate, Romain Marlin, Franck Touret, Nathalie Dereuddre‐Bosquet, Flora Donati, Francis Relouzat, Laura Junges, Mathilde Galhaut, Océane Dehan, Quentin Sconosciuti, Antoine Nougairède, Xavier de Lamballerie, Sylvie van der Werf, Roger Le Grand,
The SARS-CoV2 Omicron variants have acquired new Spike mutations leading to escape from the most of the currently available monoclonal antibody treatments reducing the options for patients suffering from severe Covid-19. Recently, both
Tópico(s): SARS-CoV-2 detection and testing
2023 - Elsevier BV | Heliyon
Nerea Zabaleta, Urja Bhatt, Cécile Hérate, Pauline Maisonnasse, Julio Sanmiguel, Cheikh Tacko Diop, Sofia Castore, Reynette Estelien, Dan Li, Nathalie Dereuddre‐Bosquet, Mariangela Cavarelli, Anne-Sophie Gallouët, Quentin Pascal, Thibaut Naninck, Nidhal Kahlaoui, Julien Lemaître, Francis Relouzat, Giuseppe Ronzitti, Hendrik Jan Thibaut, Emanuele Montomoli, James M. Wilson, Roger Le Grand, Luk H. Vandenberghe,
The COVID-19 pandemic continues to have devastating consequences on health and economy, even after the approval of safe and effective vaccines. Waning immunity, the emergence of variants of concern, breakthrough infections, and lack of global vaccine access and acceptance perpetuate the epidemic. Here, we demonstrate that a single injection of an adenoassociated virus (AAV)-based COVID-19 vaccine elicits at least 17-month-long neutralizing antibody responses in non-human primates at levels that were ...
Tópico(s): Immunotherapy and Immune Responses
2022 - Elsevier BV | Molecular Therapy
Aurélien Marc, Romain Marlin, Flora Donati, Mélanie Prague, Marion Kerioui, Cécile Hérate, Marie Alexandre, Nathalie Dereuddre‐Bosquet, Julie Bertrand, Vanessa Contreras, Sylvie Behillil, Pauline Maisonnasse, Sylvie van der Werf, Roger Le Grand, Jérémie Guedj,
The impact of variants of concern (VoC) on SARS-CoV-2 viral dynamics remains poorly understood and essentially relies on observational studies subject to various sorts of biases. In contrast, experimental models of infection constitute a powerful model to perform controlled comparisons of the viral dynamics observed with VoC and better quantify how VoC escape from the immune response. Here we used molecular and infectious viral load of 78 cynomolgus macaques to characterize in detail the effects ...
Tópico(s): Infectious Encephalopathies and Encephalitis
2023 - Public Library of Science | PLoS Computational Biology
Paule Hermet, Benoît Delache, Cécile Hérate, Esther Wolf, Gaily Kivi, Erkki Juronen, Karl Mumm, Eva Žusinaite, Denis E. Kainov, Eve Sankovski, Kai Virumäe, Anu Planken, Andres Merits, Jessica E. Besaw, A.W. Yee, Takefumi Morizumi, Kyumhyuk Kim, Anling Kuo, Asma Berriche, Nathalie Dereuddre‐Bosquet, Quentin Sconosciuti, Thibaut Naninck, Francis Relouzat, Mariangela Cavarelli, Mart Ustav, Derek J. Wilson, Oliver P. Ernst, Andres Männik, R. Legrand, Mart Ustav,
The COVID-19 pandemic represents a global challenge that has impacted and is expected to continue to impact the lives and health of people across the world for the foreseeable future. The rollout of vaccines has provided highly anticipated relief, but effective therapeutics are required to further reduce the risk and severity of infections. Monoclonal antibodies have been shown to be effective as therapeutics for SARS-CoV-2, but as new variants of concern (VoC) continue to emerge, their utility and ...
Tópico(s): Inhalation and Respiratory Drug Delivery
2023 - Public Library of Science | PLoS Pathogens
Thibaut Naninck, Nidhal Kahlaoui, Julien Lemaître, Pauline Maisonnasse, Antoine De Mori, Quentin Pascal, Vanessa Contreras, Romain Marlin, Francis Relouzat, Benoît Delache, Cécile Hérate, Yoann Aldon, Marit J. van Gils, Nerea Zabaleta, Raphaël Ho Tsong Fang, Nathalie Bosquet, Rogier W. Sanders, Luk H. Vandenberghe, Catherine Chapon, Roger Le Grand,
Non-human primates (NHPs) are particularly relevant as preclinical models for SARS-CoV-2 infection and nuclear imaging may represent a valuable tool for monitoring infection in this species. We investigated the benefit of computed X-ray tomography (CT) and [18F]-FDG positron emission tomography (PET) to monitor the early phase of the disease in a large cohort (n = 76) of SARS-CoV-2 infected macaques. Following infection, animals showed mild COVID-19 symptoms including typical lung lesions. CT scores at ...
Tópico(s): COVID-19 Clinical Research Studies
2022 - Cell Press | iScience
Cécile Hérate, Laure Sabatier,
Telomeres are composed of DNA repeat sequences at the ends of chromosomes that recruit a multitude of proteins to form a complex loop structure at each extremity. The integrity of this structure is critical and correct conformation of the loop is essential for the protection of chromosome ends from DDR signaling. The properties of telomere composition and synthesis result in telomere shortening at each cell division, programming cellular lifespan by driving aged cells towards death. Indeed, many ...
Tópico(s): Pluripotent Stem Cells Research
2020 - Elsevier BV | Current Opinion in Genetics & Development
Cécile Hérate, Patricia Brochard, Florent de Vathaire, Michelle Ricoul, Bernadette Martins, Laurence Laurier, Jean-Robert Deverre, Bertrand Thirion, Lucie Hertz‐Pannier, Laure Sabatier,
Abstract Background Magnetic resonance imaging (MRI) is currently considered a safe imaging technique because, unlike computed tomography, MRI does not expose patients to ionising radiation. However, conflicting literature reports possible genotoxic effects of MRI. We herein examine the chromosomal effects of repeated MRI scans by performing a longitudinal follow-up of chromosomal integrity in volunteers. Methods This ethically approved study was performed on 13 healthy volunteers (mean age 33 years) ...
Tópico(s): Effects of Radiation Exposure
2022 - Springer Science+Business Media | European Radiology Experimental
Craig Fenwick, Priscilla Turelli, Yoan Duhoo, Kelvin Lau, Cécile Hérate, Romain Marlin, Myriam Lamrayah, Jérémy Campos, Line Esteves-Leuenberger, Alex Farina, Charlène Raclot, Vanessa Genet, Flurin Fiscalini, Julien Cesborn, Laurent Perez, Nathalie Dereuddre‐Bosquet, Vanessa Contreras, Kyllian Lheureux, Francis Relouzat, Rana Abdelnabi, Pieter Leyssen, Yves Lévy, Florence Pojer, Roger Le Grand, Didier Trono, Giuseppe Pantaleo,
Due to the rapid evolution of SARS-CoV-2 to variants with reduced sensitivity to vaccine-induced humoral immunity and the near complete loss of protective efficacy of licensed therapeutic monoclonal antibodies, we isolated a potent, broad-spectrum neutralizing antibody that could potentially provide prophylactic protection to immunocompromised patient populations.
Tópico(s): COVID-19 Clinical Research Studies
2023 - Elsevier BV | Journal of Infection
Tamizh Selvan Gnanasekaran, Michelle Ricoul, Patricia Brochard, Cécile Hérate, Laure Sabatier,
Purpose: The premature chromosome condensation (PCC) technique is used to study exposure to external radiation through the determination of chromosome fragments observed in interphase cells. The presence of large telomeric signals in CHO cells interferes with the detection of PCC fragments and the identification of dicentric chromosomes. We present an improved method for the fusion of G0-lymphocytes with mitotic Akodon cells (few chromosomes and weakly-staining telomeric sequences) to induce PCC in ...
Tópico(s): Plant Genetic and Mutation Studies
2019 - Taylor & Francis | International Journal of Radiation Biology
Tianzhen Xu, Junxiong Fang, Huai Zhang,
... boundary layer meteorology, also some regular characteristics of herate are presented.
Tópico(s): Maritime Transport Emissions and Efficiency
1992 - | Haiyang yu huzhao
Hélène Letscher, Delphine Guilligay, Grégory Effantin, Axelle Amen, Guidenn Sulbarán, Judith A. Burger, Laëtitia Bossevot, Laura Junges, Marco Leonec, Julie Morin, Matthieu Van Tilbeurgh, Cécile Hérate, Anne-Sophie Gallouët, Francis Relouzat, Sylvie van der Werf, Mariangela Cavarelli, Nathalie Dereuddre‐Bosquet, Marit J. van Gils, Rogier W. Sanders, Pascal Poignard, Roger Le Grand, Winfríed Weissenhorn,
Tópico(s): Respiratory viral infections research
2025 - Nature Portfolio | npj Vaccines
Mamun Al-Mahtab, Osamu Yoshida, Yoichi Hiasa, Sheikh MF Akbar, Guillermo Fernández–Varo, Jorge Aguiar, Eduardo Pentón, Md. Sakirul Islam Khan, Gerardo Guillén, Julio Aguilar, Carlos A. Guzmán, Marie-Louise Michel, Maryline Bourgine, Maria A. Marrero, Stephanie Trittel, Thomas Ebensen, Peggy Riese, Roger Le Grand, Cécile Hérate, Aurélie Mauras,
The immune system plays a central role in controlling acute hepatitis B infection and in patients resolving chronic hepatitis B (CHB). Given that 221 million (75%) of CHB patients reside in low- and middle-income countries, the development of a vaccine with therapeutic properties represents a rational and cost-effective approach more than a romantic endeavor. This review systematically analyzes the key variables related to the safety, efficacy, and effectiveness of CHB treatments. HeberNasvac experience ...
Tópico(s): Hepatitis Viruses Studies and Epidemiology
2024 - | Euroasian Journal of Hepato-Gastroenterology
Urja Bhatt, Cécile Hérate, Reynette Estelien, Francis Relouzat, Nathalie Dereuddre‐Bosquet, Dawid Maciorowski, Cheikh Tacko Diop, Emma Couto, Jillian Staiti, Mariangela Cavarelli, Laëtitia Bossevot, Quentin Sconosciuti, Page Bouchard, Roger Le Grand, Luk H. Vandenberghe, Nerea Zabaleta,
PARVAX is a genetic vaccine platform based on an adeno-associated vector that has demonstrated to elicit potent, durable, and protective immunity in nonhuman primates (NHPs) after a single dose. Here, we assessed vaccine immunogenicity following a PARVAX prime-boost regimen against SARS-CoV-2. In mice, a low-dose prime followed by a higher-dose boost elicited potent neutralizing antibody responses and distinct cross-reactivity profiles, depending on the antigen used in the booster vaccine. However, ...
Tópico(s): Animal Virus Infections Studies
2024 - Multidisciplinary Digital Publishing Institute | Vaccines