Mohammad M. Al‐Sanea, Ahmad J. Obaidullah, Mohamed E. Shaker, Garri Chilingaryan, Mohammed M. Alanazi, Nawaf A. Alsaif, Hamad M. Alkahtani, Sultan A. Alsubaie, Mohamed A. Abdelgawad,
... new compound with 3-hydrazonoindolin-2-one scaffold (HI 5) was well designed, synthesized, and biologically evaluated as ... compound. Methods: The potential anti-cancerous effect of HI 5 was evaluated using cytotoxicity assay, flow cytometric analysis ... virtual docking studies. Results: The results revealed that HI 5 exhibits pronounced CDK2 inhibitory activity and cytotoxicity in ... cancer MCF-7 cell line. The cytotoxicity of HI 5 was found to be intrinsically mediated apoptosis, which ... high activation of caspase 3 and p53. Besides, HI 5 blocked the proliferation of the MCF-7 cell ...
Tópico(s): Cancer therapeutics and mechanisms
2021 - Multidisciplinary Digital Publishing Institute | Molecules
Min-Ying Wang, Shyue‐Ru Doong,
... an infectious bursal disease virus (IBDV), Trichoplusia ni (Hi-5) cells were cultured in a 500-ml spinner ... coefficient, kLa, was 24/h under these conditions. Hi-5 cells were grown and then infected with a recombinant rVP2H expressing baculovirus in spinner flasks. The Hi-5 cells generated a higher rVP2H yield at a ... from 25 to 54 mg/l when the Hi-5 cells were infected at a cell density of ... should be beneficial in research laboratories employing the Hi-5 cells/baculovirus expression system as a rapid and ...
Tópico(s): Virology and Viral Diseases
2000 - Elsevier BV | Process Biochemistry
... were incubated in vitro with radioactively-labelled histamine (Hi), 5-hydroxytryptamine (5-HT), dopamine (DA), noradrenaline (NA), tyramine ( ... the cells were compared. The release curves for Hi, 5-HT, DA, NA and TA were found to ... from that for endogenous Hi. The uptake of Hi, 5-HT, DA, PhEA, TrpA and Eph into granules ... amine content of these granules was then measured. Hi, 5-HT and DA were found to be mainly ...
Tópico(s): Amino Acid Enzymes and Metabolism
1975 - Wiley | Acta Physiologica Scandinavica
Meng‐Shiou Lee, Min-Ying Wang, Yu-Jui Tai, Su‐Yuan Lai,
... virus (IBDV) was expressed in insect Sf9 and Hi-5 cells, and the types of particles generated as ... were examined. Recombinant VPXH (rVPXH) protein, expressed in Hi-5 cells at an expression level 4× higher than ... that could form small virus-like particles in Hi-5 cells. Finally, we demonstrated that virus-neutralizing antibodies ...
Tópico(s): Vector-Borne Animal Diseases
2004 - Elsevier BV | Journal of Virological Methods
Jeong Su Oh, Pasquale Manzerra, Mary B. Kennedy,
... recombinant FLAG-tagged synGAP (rsynGAP) was expressed in Hi-5 insect cells by the Caltech Protein Expression Laboratory. ... 3 μg of purified rat brain CaMKII and Hi-5 cell membranes containing 1.5-2 μg of ... either the postsynaptic density or the membranes of Hi-5 insect cells was determined from immunoblots containing increasing ... GST fusion protein or 300-400 μg/ml Hi-5 membrane protein containing recombinant synGAP. The reaction mixture ...
Tópico(s): Ion channel regulation and function
2004 - Elsevier BV | Journal of Biological Chemistry
Stefan Gaunitz, Chunsheng Jin, Anki Nilsson, Jining Liu, Niclas G. Karlsson, Jan Holgersson,
... in insect cell lines derived from Trichoplusia ni (Hi-5) and Spodoptera frugiperda (Sf9) were characterized. The P- ... glycosylation sites was expressed and purified from the Hi-5 and Sf9 cell culture medium using affinity chromatography ... acid) were found to be prevalent. Also sulfate (Hi-5 and Sf9) and phosphocholine (PC; Sf9) O-glycan ... and with terminating N-acetylhexosamine (HexNAc) units, whereas Hi-5 produced a family of large O-glycans with ( ...
Tópico(s): Invertebrate Immune Response Mechanisms
2013 - Oxford University Press | Glycobiology
Shigeharu Kinoshita, Gen Kaneko, Jeen H. Lee, Kiyoshi Kikuchi, Hiroshi Yamada, Takeya Hara, Yasuo Itoh, Shugo Watabe,
... 20 °C for 8 h. Four genes including HI‐5 were detected as heat stress‐responsive genes by ... PCR. Cloning for full‐length cDNA sequences of HI‐5 transcripts and related genomic DNA analysis revealed that ... and HI‐5b , were transcribed from the single HI‐5 gene. While the HI‐5a protein contained a ... 5a and HI‐5b transcripts from the single HI‐5 gene in the diatom.
Tópico(s): thermodynamics and calorimetric analyses
2001 - Wiley | European Journal of Biochemistry
Anja Schmidt, Robert MacColl, Barbara Lindau‐Shepard, David R. Buckler, James A. Dias,
... and Bio-Rad.Expression of hFSHR ECD in Hi 5 Insect CellsThe expression vector used was kindly provided ... h after seeding in roller bottles 2 × 108 Hi 5 cells (Invitrogen) were infected with recombinant virus at ... FSH virus was amplified in Sf9 cells, and Hi 5 cells were infected for expression using a titer ... the antibody for 1 h at 37 °C (Hi 5 cell-conditioned media was used as control). To ...
Tópico(s): Hormonal and reproductive studies
2001 - Elsevier BV | Journal of Biological Chemistry
Meng‐Shiou Lee, Shyue‐Ru Doong, S.-Y. Lai, Jeong‐Yau Ho, Manying Wang,
... present study, another insect cell line, High-Five (Hi-5) cells, was demonstrated to efficiently produce VLPs. Meanwhile, ... processed to the matured form of VP2. Consequently, Hi-5 cells are better in terms of polyprotein processing ...
Tópico(s): Mosquito-borne diseases and control
2006 - American Chemical Society | Biotechnology Progress
Charlotte Jeanneau, V. Chazalet, Claudine Augé, Dikeos Mario Soumpasis, Anne Harduin‐Lepers, Philippe Delannoy, Anne Imberty, Christelle Breton,
... the culture medium upon transfection of Sf9 or Hi-5™ cells with the recombinant baculoviruses. Although they differ ... activity being observed for ST3-Δ56 expressed in Hi-5™ cells (data not shown). Therefore, the ST3-Δ56 construct overexpressed in Hi-5 cells was selected to further explore the function ...
Tópico(s): Galectins and Cancer Biology
2004 - Elsevier BV | Journal of Biological Chemistry
... and the average of the five highest readings (Hi-5). The range of the GM magnetic field magnitude ... sources were highly variable across and within facilities (Hi-5 range: 1.0–530 μT). Chemical and Allied ... Products (SIC 33) had facilities with GM and Hi-5 magnetic fields greater than any of the other ...
Tópico(s): Electromagnetic Compatibility and Measurements
2000 - Oxford University Press | The Annals of Occupational Hygiene
Lea Schäfer, Sarah M. Grundmann, Garima Maheshwari, Marcus Höring, Gerhard Liebisch, Erika Most, Klaus Eder, Robert Ringseis,
... HI-2.5, n = 35) or 5.0% (HI-5.0, n = 35) Hermetia illucens (HI) larvae fat ... Coriobacteriales and family Eggerthellaceae were lower in group HI-5.0 compared to group HI-2.5 ( P < ... 25 transcripts to be differentially expressed between groups HI-5.0 vs. HI-0 and groups HI-2. ...
Tópico(s): Animal Nutrition and Physiology
2023 - BioMed Central | Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology
A. Mank, Markus Drescher, T. Huth‐Fehre, G. Schönhense, N. Böwering, U. Heinzmann,
... of the photoelectron angular distribution of the process HI(5 pi 4) 1 Sigma + ( upsilon "=0)+hv to HI+(5 pi 3) 2 Pi 3/2 ( upsilon '=0)+ ...
Tópico(s): Advanced Chemical Physics Studies
1989 - IOP Publishing | Journal of Physics B Atomic Molecular and Optical Physics
Richard W. Nesto, Rodrigo M. Lago,
... In the Hyperglycemia: Intensive Insulin Infusion in Infarction (HI-5) study,23 an insulin infusion regimen designed to ... M. The Hyperglycemia: Intensive Insulin Infusion in Infarction (HI-5) study: a randomized controlled trial of insulin infusion ...
Tópico(s): Metabolism, Diabetes, and Cancer
2008 - Lippincott Williams & Wilkins | Circulation
Guillermo Garcia‐Manero, Steven D. Gore, Christopher R. Cogle, Elias Jabbour, M. Renee Ward, Kyle J. MacBeth, Eric Laille, Heidi Giordano, Hagop M. Kantarjian, Barry Skikne,
... 21-day QD Overall response* (CR, mCR, any HI) 5/6 (83) 3/3 (100) CR 0/6 ... 3 (67) mCR 0/6 3/3 (100) HI 5/6 (83) 3/3 (100) HI-erythroid 3/ ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2010 - Elsevier BV | Blood
Sokol Haxhinasto, Gail A. Bishop,
... cells (K1) expressing mCD154 and human CD154-expressing HI-5 insect cells have been described (15.Hostager B. ... specific F(ab′)2 and/or mCD154 expressing HI-5 cells for 15 min. Cells were lysed, and ...
Tópico(s): Immune Cell Function and Interaction
2004 - Elsevier BV | Journal of Biological Chemistry
Vincent Wong, Mark McLean, Steven Boyages, N. Wah Cheung,
... status. The Hyperglycemia: Intensive Insulin Infusion In Infarction (HI-5) study is a multicentered randomized trial of insulin ... effect of insulin-based infusion therapy. In the HI-5 study, patients presenting with AMI within 24 h ...
Tópico(s): Cardiovascular Function and Risk Factors
2004 - American Diabetes Association | Diabetes Care
Bing Chen, Genfa Zhou, Mikyung Kim, Yasmin Chishti, Rebecca E. Hussey, Barry K. Ely, J.J. Skehel, Ellis L. Reinherz, Stephen C. Harrison, Don C. Wiley,
... in 100-ml spinner flasks. Infected Trichoplusia ni (Hi-5) cells were found to secrete significantly more SIV ... scale protein production, 10 liters of T. ni (Hi-5) cells (2 × 106 cells/ml) in Ex-Cell ...
Tópico(s): HIV/AIDS Research and Interventions
2000 - Elsevier BV | Journal of Biological Chemistry
Manying Wang, Yongbo Yang, Mei–Hsuan Lee, S.-Y. Lai,
... derived from Spodoptera litura, Sf9, and High Five (Hi-5) cells, were used for the production of pro- ... cells is cell-line-dependent, because proteases in Hi-5 or Sf9 cells could not process pro-HGF ...
Tópico(s): Pancreatic function and diabetes
2000 - American Chemical Society | Biotechnology Progress
Bhuvana Gopalakrishnan, Subbaratnam Muthukrishnan, Karl J. Kramer,
... recombinant chitinase in SF9, SF21 and Trichoplusia ni (Hi-5) cell lines showed that the yields were in the order Hi-5 > SF21 > SF9. Chitinase accumulated in hemolymph after injection ...
Tópico(s): Entomopathogenic Microorganisms in Pest Control
1995 - Elsevier BV | Insect Biochemistry and Molecular Biology
Yu‐Shen Cheng, Meng‐Shiou Lee, Su‐Yuan Lai, Shyue‐Ru Doong, Min‐Ying Wang,
... infection. The particles within the infected High‐Five (Hi‐5) cell lysates were partially purified by employing immobilized ... particles was routinely obtained from a 500 mL Hi‐5 cell culture broth. The separation of the particulate ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2001 - American Chemical Society | Biotechnology Progress
Kenneth R. Stark, Anthony A. James,
... virus for recombinant protein production was expanded in Hi-5 cells (Invitrogen). Recombinant protein (580 μg of total protein) was harvested from the supernatant of infected Hi-5 cells, and protease inhibitor mixture was added. The ...
Tópico(s): Insect symbiosis and bacterial influences
1998 - Elsevier BV | Journal of Biological Chemistry
Yi‐Ling Lin, Mahmud K. K. Shivji, Clark Chen, Richard D. Kolodner, Richard D. Wood, Anindya Dutta,
... Fanning. DNA polymerase α/primase was expressed in Hi-5 cells with baculovirus infection and purified by immunoaffinity ... T Ag was also purified from baculovirus-infected Hi-5 cells by immunoaffinity column chromatography with monoclonal Ab ...
Tópico(s): RNA Research and Splicing
1998 - Elsevier BV | Journal of Biological Chemistry
Richard L. Summers, James C. Kolb, LouAnn H. Woodward, Robert Galli,
... patients were found to have only systolic dysfunction (HI < 5 and IVRT < 0.125), while 19% had a predominantly diastolic etiology (IVRT > 0.125 and HI > 5) for their failure. Conclusion: IC measures of contractility ...
Tópico(s): Body Composition Measurement Techniques
1999 - Wiley | Academic Emergency Medicine
А. В. Лыжина, Roman V. Buzinov, T. N. Unguryanu, А. Б. Гудков,
... systems, skin (HI = 4.3), the hormonal exchange (HI = 5.2) and the digestive apparatus (HI = 5.3). The total cancer risk was high ^R = ...
Tópico(s): Human Health and Disease
2012 - ECO-vector | Ekologiya Cheloveka (Human Ecology)
Tomoshige Ando, Nujarin Jongruja, Nobuaki Okumura, Kosuke Morikawa, Shigenori Kanaya, Toshifumi Takao,
... decreased and those of the complexes of RNase HI: 5-mer RNA: 8-mer DNA (red), RNase HI: ... Zn2+ (green), RNase HI: DNA: Zn2+ (blue), RNase HI: 5-mer RNA/DNA: Zn2+ (red), RNase HI: 6- ...
Tópico(s): Bacterial Genetics and Biotechnology
2021 - Elsevier BV | Journal of Biological Chemistry
Anders Bergendorff, Börje Uvnäs,
... endogenous Hi and 5‐HT. The uptake of Hi, 5‐HT, TrpA, PhEA, TA, DA, NA, ACh, Na ...
Tópico(s): Polyamine Metabolism and Applications
1973 - Wiley | Acta Physiologica Scandinavica
Karel Wiesner, Thomas Y. R. Tsai, Akwasi Minta,
... H ) , 4.28 (dd, J = 6, lH, 16-HI, 5 .5 2 ( s , 2H, benzylicl, 6.3 (broad ...
Tópico(s): Phytochemistry and Bioactive Compounds
1979 - Elsevier BV | Heterocycles
The crystal structures of (phenacetin) 2 • H I 5 (triclinic, a — 12.44 A, b — 10.67, c = 5.81, oc = 103.3°, B — 103.7, y — 87.3, P1, Z = 1) and (theobromine) 2 H 2 I 8 (triclinic, a — 14.38 A, b = 14.07, c = 7.75, = 91.2°, B = 100.8, y = 91.0, P1, Z = 2) have been solved by Patterson methods, using intensities measured by Weissenberg diffractometer with graphite-monochromated Mo Ka radiation (2815 and 2827 intensities, R = 8.3 % and 11.1 % respectively). Both structures are polyiodide salts, with alternating cationic (organic) and anionic ( ...
Tópico(s): Organometallic Compounds Synthesis and Characterization
1979 - Royal Society | Philosophical Transactions of the Royal Society of London Series A Mathematical and Physical Sciences
James R. Sellers, E Eisenberg, Robert Adelstein,
... difference in the actin binding constants of unphosphorylated HI" (5.35 X 1 0 ' M-') and fully phosphoryl- ...
Tópico(s): Force Microscopy Techniques and Applications
1982 - Elsevier BV | Journal of Biological Chemistry