... rainfalls during the last stages of Baiu at Hitoyoshi in southern Kyushu. Most flat distributions are fairly ... size distribution as observed in each rainfall at Hitoyoshi, which is characterized by the numerous space density ... by Z=360R1.6 which was observed at Hitoyoshi.
Tópico(s): Meteorological Phenomena and Simulations
1974 - The Meteorological Society of Japan | Journal of the Meteorological Society of Japan Ser II
Junya Miyoshi, Takashi Ohba, Masanori Ohkuma, Takahiko Katoh, Daisuke Tanoue, Hidetaka Katabuchi,
... women in the Amakusa area, and in the Hitoyoshi-Kuma area, both in Japan. Prophylactic oral antibiotics ... the Amakusa area and of 373 in the Hitoyoshi-Kuma area, bacterial vaginosis was found in 100 (13.9%) Amakusa residents and 71 (19.0%) Hitoyoshi-Kuma residents. Prophylactic antibiotics were prescribed for 93 Amakusa residents and 39 Hitoyoshi-Kuma residents. Among subjects who underwent a periodontal ... 7% of Amakusa residents and 59.7% of Hitoyoshi-Kuma residents. Periodontal health was improved among these ...
Tópico(s): Neonatal Respiratory Health Research
2016 - De Gruyter | Journal of Perinatal Medicine
Hiroyuki Watanabe, Kimihiro Nagai, Masatoshi Yamaguchi, Tsuyomu Ikenoue, Norimasa Mori,
... at: Department of Obstetrics and Gynecology, Health Insurance Hitoyoshi General Hospital, 35 Oigami-Machi Hitoyoshi City, Kumamoto 868, Japan Search for other works ...
Tópico(s): Reproductive Biology and Fertility
1994 - Oxford University Press | Human Reproduction
Akira Akahori, Fumio Yasuda, Kiyomi Kagawa, Toru Iwao,
... tenuipes complex derived from a plant collected at Hitoyoshi were found to consist almost exclusively of neotokorogenin. ...
Tópico(s): Phytochemistry and Bioactive Compounds
1973 - Pharmaceutical Society of Japan | Chemical and Pharmaceutical Bulletin
Shigenori Aomine, Nobufumi Miyauchi,
... Kyushu. The layer is called "imogo" in the Hitoyoshi district and "akahoya" in the Miyazaki district. The ...
Tópico(s): Plant nutrient uptake and metabolism
1965 - Taylor & Francis | Soil Science & Plant Nutrition
Minoru Ōno, Hitoyoshi Ohta, Motoyuki Ohhira, Chihiro Sekiya, Masayoshi Namiki,
Des-γ-carboxyprothrombin is an abnormal prothrombin which is drastically increased in the plasma of patients with hepatocellular carcinoma. To investigate the process of the abnormal prothrombin synthesis, the amount of prothrombin precursor was measured with an enzyme-linked immunosorbent assay using a specific antibody directed to human prothrombin; the vitamin-K-dependent γ-carboxylation of prothrombin precursor was determined in human liver tissues. The tissue content of prothrombin precursor ...
Tópico(s): Vitamin C and Antioxidants Research
1990 - SAGE Publishing | Tumor Biology
Tópico(s): Geology and Paleoclimatology Research
1987 - The Meteorological Society of Japan | The Journal of the Geological Society of Japan
C. S. Hudson, Sébastien Darras, Danielle Caillol, Hitoyoshi Yasuo, Patrick Lemaire,
Ascidians are invertebrate chordates with a larval body plan similar to that of vertebrates. The ascidian larval CNS is divided along the anteroposterior axis into sensory vesicle, neck, visceral ganglion and tail nerve cord. The anterior part of the sensory vesicle comes from the a-line animal blastomeres, whereas the remaining CNS is largely derived from the A-line vegetal blastomeres. We have analysed the role of the Ras/MEK/ERK signalling pathway in the formation of the larval CNS in the ascidian, ...
Tópico(s): Echinoderm biology and ecology
2002 - The Company of Biologists | Development
Norio Takada, Jonathan L. York, J. Muse Davis, Brenda Schumpert, Hitoyoshi Yasuo, Nori Satoh, Billie J. Swalla,
The T-box transcription factor gene Brachyury is important for the differentiation of notochord in all chordates, including the ascidians Halocynthia roretzi and Ciona intestinalis. We isolated Brachyury from molgulid ascidians, which have evolved tailless larvae multiple times independently, and found the genes appear functional by cDNA sequence analyses. We then compared the expression of Mocu-Bra in tailed Molgula oculata embryos to two tailless species, Molgula occulta (Mocc-Bra) and Molgula ...
Tópico(s): Developmental Biology and Gene Regulation
2002 - Wiley | Evolution & Development
Hitoyoshi Yasuo, Noriyuki Satoh,
Ascidians are primitive chordates in which a well-organized notochord is formed in the tail of the tadpole larva. The Brachyury (T) gene in the mouse is essential for formation of mesoderm and, in particular, of notochord. We report here the expression of an ascidian homolog (As-T) of the mouse T gene. A cDNA clone for the As-T gene contains a single open reading frame that encodes a polypeptide of 471 amino acids. Although the overall degree of amino acid identity was not very high (39.9%, ascidian/ ...
Tópico(s): Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
1994 - Wiley | Development Growth & Differentiation
C. S. Hudson, Hitoyoshi Yasuo,
Developmental processes can change during evolution at many levels of the ontogeny of an individual. Embryos of solitary ascidians have a largely invariant mode of development, with fixed cleavage patterns and fate maps. Thus the cell lineages and final body plan of the two quite distantly related species considered in this review, Ciona intestinalis and Halocynthia roretzi, are highly similar. However, close comparison of the developmental mechanisms used by these two species provide examples of ...
Tópico(s): Marine Biology and Environmental Chemistry
2008 - Wiley | Biology of the Cell
Vincent Picco, C. S. Hudson, Hitoyoshi Yasuo,
Asymmetric cell divisions produce two sibling cells with distinct fates,providing an important means of generating cell diversity in developing embryos. Many examples of such cell divisions have been described, but so far only a limited number of the underlying mechanisms have been elucidated. Here,we have uncovered a novel mechanism controlling an asymmetric cell division in the ascidian embryo. This division produces one notochord and one neural precursor. Differential activation of extracellular- ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2007 - The Company of Biologists | Development
C. S. Hudson, Sonia Lotito, Hitoyoshi Yasuo,
The ascidian neural plate has a grid-like organisation, with six rows and eight columns of aligned cells, generated by a series of stereotypical cell divisions. We have defined unique molecular signatures for each of the eight cells in the posterior-most two rows of the neural plate - rows I and II. Using a combination of morpholino gene knockdown, dominant-negative forms and pharmacological inhibitors, we tested the role of three signalling pathways in defining these distinct cell identities. Nodal ...
Tópico(s): Marine Ecology and Invasive Species
2007 - The Company of Biologists | Development
C. S. Hudson, Moly Ba, Christian Rouvière, Hitoyoshi Yasuo,
Ascidians are members of the vertebrate sister group Urochordata. Their larvae exhibit a chordate body plan, which forms by a highly accelerated embryonic strategy involving a fixed cell lineage and small cell numbers. We report a detailed analysis of the specification of three of the five pairs of motoneurons in the ascidian Ciona intestinalis and show that despite well-conserved gene expression patterns and embryological outcomes compared with vertebrates, key signalling molecules have adopted ...
Tópico(s): Marine Invertebrate Physiology and Ecology
2011 - The Company of Biologists | Development
Teruyuki Kato, Yukihiro Terada, Hitoyoshi Nishimura, Toshihiko Nagai, Shunichi Koshimura,
A GPS buoy operating about 10 km west of Cape Muroto, southwest Japan, recorded the tsunami due to the 2010 Central Chile Earthquake (Mw 8.8) that occurred on 27 February, 2010. The tsunami due to the Chile Earthquake arrived at the GPS buoy almost one day after the earthquake. The first peak of the tsunami was about 12 cm above the mean sea level. The second peak arrived about one hour and 46 minutes later and was about 20 cm higher than the mean sea level, which was the highest peak among the series ...
Tópico(s): Earthquake Detection and Analysis
2011 - Springer Nature | Earth Planets and Space
Christian Sardet, Alex McDougall, Hitoyoshi Yasuo, Janet Chênevert, Gérard Prulière, Rémi Dumollard, C. S. Hudson, Céline Hebras, Ngan Le Nguyen, Alexandre Paix,
Ascidians (marine invertebrates: urochordates) are thought to be the closest sister groups of vertebrates. They are particularly attractive models because of their non-duplicated genome and the fast and synchronous development of large populations of eggs into simple tadpoles made of about 3,000 cells. As a result of stereotyped asymmetric cleavage patterns all blastomeres become fate restricted between the 16- and 110 cell stage through inheritance of maternal determinants and/or cellular interactions. ...
Tópico(s): Marine Biology and Environmental Chemistry
2011 - Springer Science+Business Media | Methods in molecular biology
Thomas H. Meedel, Patrick Chang, Hitoyoshi Yasuo,
The activity of myogenic regulatory factor (MRF) genes is essential for vertebrate muscle development, whereas invertebrate muscle development is largely independent of MRF function. This difference indicates that myogenesis is controlled by distinct regulatory mechanisms in these two groups of animals. Here we used overexpression and gene knockdown to investigate the role in embryonic myogenesis of the single MRF gene of the invertebrate chordate Ciona intestinalis (Ci-MRF). Injection of Ci-MRF mRNA ...
Tópico(s): Marine Biology and Environmental Chemistry
2006 - Elsevier BV | Developmental Biology
Masaharu Ishihara, Sunao Kojima, Tomohiro Sakamoto, Yujiro Asada, Kazuo Kimura, Shunichi Miyazaki, Masakazu Yamagishi, Chuwa Tei, Hisatoyo Hiraoka, Masahiro Sonoda, Kazufumi Tsuchihashi, Nobuo Shinoyama, Takashi Honda, Yasuhiro Ogata, Hisao Ogawa,
... Kimura (Arao City Hospital), K. Obata (Health Insurance Hitoyoshi General Hospital), H. Shimomura (Fukuoka Tokushukai Medical Center), ...
Tópico(s): Sepsis Diagnosis and Treatment
2006 - Elsevier BV | The American Journal of Cardiology
C. S. Hudson, Hitoyoshi Yasuo,
The notochord is one of the defining features of chordates. The ascidian notochord is a rod like structure consisting of a single row of 40 cells. The anterior 32 ;primary' notochord cells arise from the A-line (anterior vegetal) blastomeres of the eight-cell stage embryo, whereas the posterior 8 ;secondary' notochord cells arise from the B-line (posterior vegetal) blastomeres of the eight-cell stage embryo. Specification of notochord precursors within these two lineages occurs in a spatially and temporally ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2006 - The Company of Biologists | Development
Hitoyoshi Yasuo, C. S. Hudson,
Fibroblast growth factor (FGF) signalling has been implicated in the generation of mesoderm and neural fates in chordate embryos including ascidians and vertebrates. In Ciona, FGF9/16/20 has been implicated in both of these processes. However, in FGF9/16/20 knockdown embryos, notochord fate recovers during later development. It is thus not clear if FGF signalling is an essential requirement for notochord specification in Ciona embryos. We show that FGF–MEK–ERK signals act during two distinct phases ...
Tópico(s): Fibroblast Growth Factor Research
2006 - Elsevier BV | Developmental Biology
Koki Nishitsuji, Takeo Horie, Aoi Ichinose, Yasunori Sasakura, Hitoyoshi Yasuo, Takehiro Kusakabe,
The tunicate Ciona intestinalis larva has a simple central nervous system (CNS), consisting of fewer than 400 cells, which is homologous to the vertebrate CNS. Recent studies have revealed neuronal types and networks in the larval CNS of C. intestinalis , yet their cell lineage and the molecular mechanism by which particular types of neurons are specified and differentiate remain poorly understood. Here, we report cell lineage origin and a cis ‐regulatory module for the anterior caudal inhibitory neurons ( ...
Tópico(s): Photoreceptor and optogenetics research
2012 - Wiley | Development Growth & Differentiation
Hitoyoshi Yasuo, Noriyuki Satoh,
Tópico(s): Single-cell and spatial transcriptomics
1993 - Nature Portfolio | Nature
C. S. Hudson, Hitoyoshi Yasuo,
Ascidians are invertebrate chordates with a simple larval tadpole form containing a notochord and an overlying dorsal neural tube. As in vertebrates, the neural tube of ascidian larvae displays positional differences along the rostral-caudal and dorsal-ventral axes in terms of neuronal cell types generated, morphology and gene expression. However, how these differences are established in this simple chordate remains largely unknown. In this study, we show that a single blastomere named b6.5, which ...
Tópico(s): Marine Ecology and Invasive Species
2005 - The Company of Biologists | Development
Hitoyoshi Yasuo, Mari Kobayashi, Yoshie Shimauchi, Noriyuki Satoh,
Tópico(s): Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
1996 - Elsevier BV | Developmental Biology
Yusuke Mizukami, Motoyuki Ohhira, Akinori Matsumoto, Yukari Murazumi, Kazuhiko Murazumi, Hitoyoshi Ohia, Masumi Ohhira, Minoru Ōno, Takayoshi Miyake, I Maekawa, Yutaka Kohgo,
Tópico(s): Systemic Lupus Erythematosus Research
1996 - Springer Science+Business Media | Journal of Gastroenterology
Yuki Nakatani, Hitoyoshi Yasuo, Noriyuki Satoh, Hiroki Nishida,
ABSTRACT The tadpole larva of an ascidian develops 40 notochord cells in the center of its tail. Most of the notochord cells originate from the A-line precursors, among which inductive interactions are required for the subsequent differentiation of notochord. The presumptive-endoderm blastomeres or presumptive-notochord blastomeres themselves are inducers of notochord formation. Notochord induction takes place during the 32-cell stage. In amphibia, mesoderm induction is thought to be mediated by ...
Tópico(s): Cancer-related gene regulation
1996 - The Company of Biologists | Development
Takako Nakagawa, Akira Hirata, Nobuhiro Inoue, Yoichiro Hashimoto, Hidenobu Tanihara,
... A 43-year-old woman was referred to Hitoyoshi General Hospital because of severe visual loss in ...
Tópico(s): Cerebrovascular and Carotid Artery Diseases
2004 - Wiley | Acta Ophthalmologica Scandinavica
Hitoyoshi Yamashita, Masamune Morita, Haruka Sugiura, Kei Fujiwara, Hiroaki Onoe, Masahiro Takinoue,
We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments.
Tópico(s): Electrowetting and Microfluidic Technologies
2014 - Elsevier BV | Journal of Bioscience and Bioengineering
Nicolas Haupaix, Philip B. Abitua, Cathy Sirour, Hitoyoshi Yasuo, Michael Levine, C. S. Hudson,
Recent evidence suggests that ascidian pigment cells are related to neural crest-derived melanocytes of vertebrates. Using live-imaging, we determine a revised cell lineage of the pigment cells in Ciona intestinalis embryos. The neural precursors undergo successive rounds of anterior–posterior (A–P) oriented cell divisions, starting at the blastula 64-cell stage. A previously unrecognized fourth A–P oriented cell division in the pigment cell lineage leads to the generation of the post-mitotic pigment ...
Tópico(s): Hippo pathway signaling and YAP/TAZ
2014 - Elsevier BV | Developmental Biology
C. S. Hudson, Narudo Kawai, Takefumi Negishi, Hitoyoshi Yasuo,
Summaryβ-catenin is a transcriptional cofactor mediating the "canonical" Wnt signaling pathway, which activates target genes in a complex with TCF (LEF) transcription factors [1]. In many metazoans, embryos are first subdivided during early cleavage stages into nuclear β-catenin-positive and -negative domains, with β-catenin specifying endoderm or mesendoderm fate. This process has been demonstrated in a wide range of phyla including cnidarians, nemerteans, and invertebrate deuterostomes (echinoderms, ...
Tópico(s): Marine Biology and Environmental Chemistry
2013 - Elsevier BV | Current Biology