Ilia G. Denisov, Thomas M. Makris, Stephen G. Sligar, Ilme Schlichting,
... email protected].More by Stephen G. Sligar, and Ilme Schlichting*Ilme SchlichtingDepartments of Biochemistry and Chemistry, Center for Biophysics ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2005 - American Chemical Society | Chemical Reviews
... Sangkee Rhee in David Davies' group and by Ilme Schlichting's group. These structures demonstrate the presence of ... Anderson, Kenneth Johnson, Katsuhide Yutani, Andrea Mozzarelli, and Ilme Schlichting. I developed many professional contacts at meetings, including ...
Tópico(s): Biochemical Acid Research Studies
2013 - Elsevier BV | Journal of Biological Chemistry
Jochen Reinstein, Ilme Schlichting, Alfred Wittinghofer,
... loop of adenylate kinase of Escherichia coliJochen Reinstein, Ilme Schlichting, and Alfred WittinghoferCite this: Biochemistry 1990, 29, 32, ...
Tópico(s): Bacterial Genetics and Biotechnology
1990 - American Chemical Society | Biochemistry
Thomas R. M. Barends, A. Gorel, Swarnendu Bhattacharyya, Giorgio Schirò, Camila Bacellar, Claudio Cirelli, Jacques‐Philippe Colletier, L. Foucar, Marie Luise Grünbein, Elisabeth Hartmann, M. Hilpert, James M. Holton, Philip J. M. Johnson, Marco Kloos, G. Knopp, Bogdan Marekha, Karol Nass, Gabriela Nass Kovács, D. Ozerov, M. Stricker, Martin H. Weik, R. Bruce Doak, Robert L. Shoeman, Christopher J. Milne, Miquel Huix‐Rotllant, Marco Cammarata, Ilme Schlichting,
Abstract High-intensity femtosecond pulses from an X-ray free-electron laser enable pump–probe experiments for the investigation of electronic and nuclear changes during light-induced reactions. On timescales ranging from femtoseconds to milliseconds and for a variety of biological systems, time-resolved serial femtosecond crystallography (TR-SFX) has provided detailed structural data for light-induced isomerization, breakage or formation of chemical bonds and electron transfer 1,2 . However, all ultrafast ...
Tópico(s): Spectroscopy and Quantum Chemical Studies
2024 - Nature Portfolio | Nature
Xiang Li, Artem Rudenko, M. S. Schöffler, N. Anders, Th. Baumann, S. Eckart, Benjamin Erk, A. De Fanis, K. Fehre, R. Dörner, L. Foucar, Sven Grundmann, Patrik Grychtol, Alexander Hartung, M. Hofmann, Markus Ilchen, Ch. Janke, Gregor Kastirke, M. Kircher, Katharina Kubiček, M. Kunitski, Tommaso Mazza, Severin Meister, N. Melzer, J. Montaño, Valerija Music, G. Nalin, Yevheniy Ovcharenko, C. Passow, A. Pier, Nils Rennhack, J. Rist, Daniel E. Rivas, Ilme Schlichting, L. Ph. H. Schmidt, Ph. Schmidt, J. Siebert, N. Strenger, D. Trabert, Florian Trinter, Isabel Vela-Peréz, R. Wagner, Peter Walter, M. Weller, Paweł Ziółkowski, A. Czasch, Daniel Rolles, Michael Meyer, T. Jahnke, Rebecca Boll,
Ultrashort x-ray pulses from free-electron lasers can efficiently charge up and trigger the full fragmentation of molecules. By coincident detection of up to five ions resulting from rapid Coulomb explosion of highly charged iodomethane, we show that the full three-dimensional equilibrium geometry of this prototypical polyatomic system can be determined from the measured ion momenta with the help of a charge buildup model. Supported by simulations of how the ion momenta would reflect specific changes ...
Tópico(s): Laser-Plasma Interactions and Diagnostics
2022 - American Physical Society | Physical Review Research
Guillaume Tetreau, M.R. Sawaya, Elke De Zitter, Elena A. Andreeva, Anne‐Sophie Banneville, Natalie A. Schibrowsky, Nicolas Coquelle, Aaron S. Brewster, Marie Luise Grünbein, Gabriela Nass Kovács, Mark S. Hunter, Marco Kloos, Raymond G. Sierra, Giorgio Schirò, Pei Qiao, Myriam Stricker, Dennis K. Bideshi, I.D. Young, Ninon Zala, Sylvain Engilberge, Alexander Gorel, Luca Signor, Jean‐Marie Teulon, M. Hilpert, L. Foucar, Johan Bielecki, Richard Bean, Raphaël de Wijn, Tokushi Sato, Henry Kirkwood, Romain Letrun, A. Batyuk, I. Snigireva, Daphna Fenel, Robin Schubert, Ethan Canfield, Mario Alba, Fréderic Laporte, Laurence Després, Maria Bacia, Amandine Roux, Christian Chapelle, François Riobé, Olivier Maury, Wai Li Ling, Sébastien Boutet, Adrian P. Mancuso⋈, Irina Gutsche, Éric Girard, Thomas R. M. Barends, Jean‐Luc Pellequer, Hyunwoo Park, Arthur Laganowsky, José A. Rodríguez, Manfred Burghammer, Robert L. Shoeman, R. Bruce Doak, Martin H. Weik, Nicholas K. Sauter, Brian A. Federici, Duilio Cascio, Ilme Schlichting, Jacques‐Philippe Colletier,
Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength ...
Tópico(s): Insect and Pesticide Research
2022 - Nature Portfolio | Nature Communications
Thomas R. M. Barends, Benjamin Stauch, Vadim Cherezov, Ilme Schlichting,
With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing time-resolved studies with unprecedented temporal resolution. This primer aims to assist structural biology groups with little or no experience in serial ...
Tópico(s): Mass Spectrometry Techniques and Applications
2022 - Nature Portfolio | Nature Reviews Methods Primers
Rebecca Boll, Julia Schäfer, Benoît Richard, K. Fehre, Gregor Kastirke, Zoltán Jurek, M. S. Schöffler, Malik Muhammad Abdullah, N. Anders, T. Baumann, S. Eckart, Benjamin Erk, A. De Fanis, R. Dörner, Sven Grundmann, Patrik Grychtol, Alexander Hartung, M. Hofmann, Markus Ilchen, Ludger Inhester, Christian Jänke, Rui Jin, M. Kircher, Katharina Kubiček, M. Kunitski, Xiang Li, Tommaso Mazza, Severin Meister, N. Melzer, J. Montaño, Valerija Music, G. Nalin, Yevheniy Ovcharenko, Christopher Passow, A. Pier, Nils Rennhack, J. Rist, Daniel E. Rivas, Daniel Rolles, Ilme Schlichting, Lothar Schmidt, Philipp Schmidt, J. Siebert, N. Strenger, D. Trabert, Florian Trinter, Isabel Vela-Peréz, R. Wagner, Peter Walter, M. Weller, Pawel Ziółkowski, Sang-Kil Son, Artem Rudenko, Michael Meyer, Robin Santra, T. Jahnke,
Abstract Following structural dynamics in real time is a fundamental goal towards a better understanding of chemical reactions. Recording snapshots of individual molecules with ultrashort exposure times is a key ingredient towards this goal, as atoms move on femtosecond (10 −15 s) timescales. For condensed-phase samples, ultrafast, atomically resolved structure determination has been demonstrated using X-ray and electron diffraction. Pioneering experiments have also started addressing gaseous samples. ...
Tópico(s): Ion-surface interactions and analysis
2022 - Nature Portfolio | Nature Physics
Robert L. Shoeman, Elisabeth Hartmann, Ilme Schlichting,
Tópico(s): X-ray Spectroscopy and Fluorescence Analysis
2022 - Nature Portfolio | Nature Protocols
Marie Luise Grünbein, A. Gorel, L. Foucar, Sergio Carbajo, William Colocho, S. Gilevich, Elisabeth Hartmann, M. Hilpert, Mark S. Hunter, Marco Kloos, Jason E. Koglin, Thomas J. Lane, Jim Lewandowski, Alberto Lutman, Karol Nass, Gabriela Nass Kovács, C.M. Roome, J.C. Sheppard, Robert L. Shoeman, M. Stricker, Tim van Driel, Sharon Vetter, R. Bruce Doak, Sébastien Boutet, Andrew Aquila, Franz Josef Decker, Thomas R. M. Barends, Claudiu A. Stan, Ilme Schlichting,
Abstract X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment ...
Tópico(s): Porphyrin Metabolism and Disorders
2021 - Nature Portfolio | Nature Communications
Damien Sorigué, Kyprianos Hadjidemetriou, Stéphanie Blangy, Guillaume Gotthard, Adeline Bonvalet, Nicolas Coquelle, Poutoum-Palakiyem Samire, Alexey Aleksandrov, Laura Antonucci, A. Benachir, Sébastien Boutet, Martin Byrdin, Marco Cammarata, Sergio Carbajo, Stephan Cuiné, R. Bruce Doak, L. Foucar, A. Gorel, Marie Luise Grünbein, Elisabeth Hartmann, Rainer Hienerwadel, M. Hilpert, Marco Kloos, Thomas J. Lane, Bertrand Légeret, Pierre Legrand, Yonghua Li‐Beisson, Solène Moulin, Didier Nurizzo, Gilles Peltier, Giorgio Schirò, Robert L. Shoeman, Michel Sliwa, Xavier Solinas, Bo Zhuang, Thomas R. M. Barends, Jacques‐Philippe Colletier, M. Joffre, Antoine Royant, Catherine Berthomieu, Martin H. Weik, Tatiana Domratcheva, Klaus Brettel, Marten H. Vos, Ilme Schlichting, Pascal Arnoux, Pavel Müller, Fred Beisson,
Light makes light work of fatty acids Photosynthetic organisms are notable for their ability to capture light energy and use it to power biosynthesis. Some algae have gone a step beyond photosynthesis and can use light to initiate enzymatic photodecarboxylation of fatty acids, producing long-chain hydrocarbons. To understand this transformation, Sorigué et al. brought to bear an array of structural, computational, and spectroscopic techniques and fully characterized the catalytic cycle of the enzyme. ...
Tópico(s): Enzyme Catalysis and Immobilization
2021 - American Association for the Advancement of Science | Science
Tadeo Moreno Chicano, Lea Dietrich, Naomi M. de Almeida, M. Akram, Elisabeth Hartmann, F. Leidreiter, Daniel Leopoldus, Melanie Mueller, Ricardo Sánchez, Guylaine H. L. Nuijten, Joachim Reimann, Kerstin-Anikó Seifert, Ilme Schlichting, Laura van Niftrik, Mike S. M. Jetten, Andreas Dietl, Boran Kartal, Kristian Parey, Thomas R. M. Barends,
Nitrate is an abundant nutrient and electron acceptor throughout Earth's biosphere. Virtually all nitrate in nature is produced by the oxidation of nitrite by the nitrite oxidoreductase (NXR) multiprotein complex. NXR is a crucial enzyme in the global biological nitrogen cycle, and is found in nitrite-oxidizing bacteria (including comammox organisms), which generate the bulk of the nitrate in the environment, and in anaerobic ammonium-oxidizing (anammox) bacteria which produce half of the dinitrogen ...
Tópico(s): Microbial Fuel Cells and Bioremediation
2021 - Nature Portfolio | Nature Microbiology
A. Gorel, Ilme Schlichting, Thomas R. M. Barends,
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) is a novel tool in structural biology. In contrast to conventional crystallography, SFX relies on merging partial intensities acquired with X-ray beams of often randomly fluctuating properties from a very large number of still diffraction images of generally randomly oriented microcrystals. For this reason, and possibly due to limitations of the still evolving data-analysis programs, XFEL-derived SFX data are typically ...
Tópico(s): Photosynthetic Processes and Mechanisms
2021 - International Union of Crystallography | IUCrJ
Karol Nass, A. Gorel, Malik Muhammad Abdullah, Andrew V. Martin, Marco Kloos, Agostino Marinelli, Andrew Aquila, Thomas R. M. Barends, Franz-Josef Decker, R. Bruce Doak, L. Foucar, Elisabeth Hartmann, M. Hilpert, Mark S. Hunter, Zoltán Jurek, Jason E. Koglin, Alexander Kozlov, Alberto Lutman, Gabriela Nass Kovács, C.M. Roome, Robert L. Shoeman, Robin Santra, Harry M. Quiney, Beata Ziaja, Sébastien Boutet, Ilme Schlichting,
X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological samples. Indeed, correlated movements of iron and sulfur ions were observed in XFEL-irradiated ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2020 - Nature Portfolio | Nature Communications
Marie Luise Grünbein, M. Stricker, Gabriela Nass Kovacs, Marco Kloos, R. Bruce Doak, Robert L. Shoeman, Jochen Reinstein, Sylvain Lecler, Stefan Haacke, Ilme Schlichting,
Tópico(s): Advanced X-ray Imaging Techniques
2020 - Nature Portfolio | Nature Methods
Joyce Woodhouse, Gabriela Nass Kovács, Nicolas Coquelle, Lucas M. Uriarte, Virgile Adam, Thomas R. M. Barends, Martin Byrdin, E. De la Mora, R. Bruce Doak, Mikołaj Feliks, Martin J. Field, Franck Fieschi, Virginia Guillon, Stefan Jakobs, Yasumasa Joti, Pauline Machebœuf, Koji Motomura, Karol Nass, Shigeki Owada, C.M. Roome, Cyril Ruckebusch, Giorgio Schirò, Robert L. Shoeman, Michel Thépaut, Tadashi Togashi, Kensuke Tono, Makina Yabashi, Marco Cammarata, L. Foucar, Dominique Bourgeois, Michel Sliwa, Jacques‐Philippe Colletier, Ilme Schlichting, Martin H. Weik,
Abstract Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced fluorescence imaging. Photoswitching from a non-fluorescent off - state to a fluorescent on - state involves trans -to- cis chromophore isomerization and proton transfer. Whereas excited-state events on the ps timescale have been structurally characterized, conformational changes on slower timescales remain elusive. Here we describe the off-to-on photoswitching mechanism in the RSFP rsEGFP2 by using a combination ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2020 - Nature Portfolio | Nature Communications
Gabriela Nass Kovács, Jacques‐Philippe Colletier, Marie Luise Grünbein, Yang Yang, Till Stensitzki, A. Batyuk, Sergio Carbajo, R. Bruce Doak, D. Ehrenberg, L. Foucar, Raphael Gasper, A. Gorel, M. Hilpert, Marco Kloos, Jason E. Koglin, Jochen Reinstein, C.M. Roome, Ramona Schlesinger, Matthew Seaberg, Robert L. Shoeman, M. Stricker, Sébastien Boutet, Stefan Haacke, Joachim Heberle, Karsten Heyne, Tatiana Domratcheva, Thomas R. M. Barends, Ilme Schlichting,
Abstract Bacteriorhodopsin (bR) is a light-driven proton pump. The primary photochemical event upon light absorption is isomerization of the retinal chromophore. Here we used time-resolved crystallography at an X-ray free-electron laser to follow the structural changes in multiphoton-excited bR from 250 femtoseconds to 10 picoseconds. Quantum chemistry and ultrafast spectroscopy were used to identify a sequential two-photon absorption process, leading to excitation of a tryptophan residue flanking ...
Tópico(s): Photosynthetic Processes and Mechanisms
2019 - Nature Portfolio | Nature Communications
Marie Luise Grünbein, Johan Bielecki, A. Gorel, M. Stricker, Richard Bean, Marco Cammarata, Katerina Dörner, Lars Fröhlich, Elisabeth Hartmann, Steffen Hauf, M. Hilpert, Yoonhee Kim, Marco Kloos, Romain Letrun, M. Messerschmidt, Grant Mills, Gabriela Nass Kovács, Marco Ramilli, C.M. Roome, Tokushi Sato, Matthias Scholz, Michel Sliwa, J. Sztuk-Dambietz, Martin H. Weik, Britta Weinhausen, Nasser Al-Qudami, Djelloul Boukhelef, Sándor Brockhauser, Wajid Ehsan, Moritz Emons, Sergey Esenov, Hans Fangohr, Alexander Kaukher, Thomas Kluyver, Max Lederer, Luis Maia, Maurizio Manetti, Thomas Michelat, Astrid Münnich, Florent Pallas, Guido Palmer, Gianpietro Previtali, Natascha Raab, A. Silenzi, J. Szuba, Sandhya Venkatesan, K. Wrona, Jun Zhu, R. Bruce Doak, Robert L. Shoeman, L. Foucar, Jacques‐Philippe Colletier, A. Mancuso, Thomas R. M. Barends, Claudiu A. Stan, Ilme Schlichting,
Abstract X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10–120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage ...
Tópico(s): Advanced X-ray Imaging Techniques
2018 - Nature Portfolio | Nature Communications
Benjamin Erk, Jan Philippe Müller, Cédric Bomme, Rebecca Boll, Günter Brenner, Henry N. Chapman, Jonathan Correa, S. Düsterer, Siarhei Dziarzhytski, Stefan Eisebitt, H. Graafsma, Sören Grunewald, Lars Gumprecht, R. Hartmann, Günter Hauser, Barbara Keitel, Clemens von Korff Schmising, Marion Kuhlmann, Bastian Manschwetus, Laurent Mercadier, Erland Müller, Christopher Passow, Elke Plönjes, Daniel Ramm, Dimitrios Rompotis, Artem Rudenko, Daniela Rupp, Mario Sauppe, Frank Siewert, Dieter Schlosser, L. Strüder, A. Swiderski, Simone Techert, K. Tiedtke, Thomas Tilp, R. Treusch, Ilme Schlichting, J. Ullrich, R. Moshammer, T. Möller, Daniel Rolles,
The non-monochromatic beamline BL1 at the FLASH free-electron laser facility at DESY was upgraded with new transport and focusing optics, and a new permanent end-station, CAMP, was installed. This multi-purpose instrument is optimized for electron- and ion-spectroscopy, imaging and pump–probe experiments at free-electron lasers. It can be equipped with various electron- and ion-spectrometers, along with large-area single-photon-counting pnCCD X-ray detectors, thus enabling a wide range of experiments ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2018 - Wiley | Journal of Synchrotron Radiation
Kanupriya Pande, Jeffrey J. Donatelli, Erik Malmerberg, L. Foucar, Christoph Bostedt, Ilme Schlichting, Petrus H. Zwart,
Significance Fluctuation X-ray scattering is a biophysical structural characterization technique that overcomes low data-to-parameter ratios encountered in traditional X-ray methods used for studying noncrystalline samples. By collecting a series of ultrashort X-ray exposures on an ensemble of particles at a free-electron laser, information-dense experimental data can be extracted that ultimately result in structures with a greater level of detail than can be obtained using traditional X-ray scattering ...
Tópico(s): Advanced X-ray Imaging Techniques
2018 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Nicolas Coquelle, Michel Sliwa, Joyce Woodhouse, Giorgio Schirò, Virgile Adam, Andrew Aquila, Thomas R. M. Barends, Sébastien Boutet, Martin Byrdin, Sergio Carbajo, E. De la Mora, R. Bruce Doak, Mikołaj Feliks, Franck Fieschi, L. Foucar, Virginia Guillon, M. Hilpert, Mark S. Hunter, Stefan Jakobs, Jason E. Koglin, G. Kovacsova, Thomas J. Lane, Bernard C. Levy, Mengning Liang, Karol Nass, J. Ridard, Joseph S. Robinson, C.M. Roome, Cyril Ruckebusch, Matthew Seaberg, Michel Thépaut, Marco Cammarata, I. Demachy, Martin J. Field, Robert L. Shoeman, Dominique Bourgeois, Jacques‐Philippe Colletier, Ilme Schlichting, Martin H. Weik,
Chromophores absorb light in photosensitive proteins and thereby initiate fundamental biological processes such as photosynthesis, vision and biofluorescence. An important goal in their understanding is the provision of detailed structural descriptions of the ultrafast photochemical events that they undergo, in particular of the excited states that connect chemistry to biological function. Here we report on the structures of two excited states in the reversibly photoswitchable fluorescent protein ...
Tópico(s): Photosynthetic Processes and Mechanisms
2017 - Nature Portfolio | Nature Chemistry
Xuanxuan Li, Chun-Ya Chiu, Hsiang-Ju Wang, Stephan Kassemeyer, Sabine Botha, Robert L. Shoeman, Robert M. Lawrence, Christopher Kupitz, Richard A. Kirian, Daniel James, Dingjie Wang, Garrett Nelson, M. Messerschmidt, Sébastien Boutet, Garth J. Williams, Elisabeth Hartmann, Aliakbar Jafarpour, L. Foucar, Anton Barty, Henry N. Chapman, Mengning Liang, Andreas Menzel, Fenglin Wang, Shibom Basu, Raimund Fromme, R. Bruce Doak, Petra Fromme, Uwe Weierstall, Michael H. Huang, John C. H. Spence, Ilme Schlichting, Brenda G. Hogue, Haiguang Liu,
Scientific Data 4:170048 doi: 10.1038/sdata201748 (2017); Published 11 April 2017; Updated 24 October 2017. The Data Descriptor incorrectly states the number of normal incidences used to generate the plot in Fig. 4b as 209. This plot was generated from 32 normal incidence cases.
Tópico(s): Advanced X-ray Imaging Techniques
2017 - Nature Portfolio | Scientific Data
Robert Lindner, Elisabeth Hartmann, Mirosław Tarnawski, Andreas Winkler, Daniel Frey, Jochen Reinstein, Anton Meinhart, Ilme Schlichting,
Light-regulated enzymes enable organisms to quickly respond to changing light conditions. We characterize a photoactivatable adenylyl cyclase (AC) from Beggiatoa sp. (bPAC) that translates a blue light signal into the production of the second messenger cyclic AMP. bPAC contains a BLUF photoreceptor domain that senses blue light using a flavin chromophore, linked to an AC domain. We present a dark state crystal structure of bPAC that closely resembles the recently published structure of the homologous ...
Tópico(s): Photosynthetic Processes and Mechanisms
2017 - Elsevier BV | Journal of Molecular Biology
G. Kovacsova, Marie Luise Grünbein, Marco Kloos, Thomas R. M. Barends, Ramona Schlesinger, Joachim Heberle, Wolfgang Kabsch, Robert L. Shoeman, R. Bruce Doak, Ilme Schlichting,
Serial (femtosecond) crystallography at synchrotron and X-ray free-electron laser (XFEL) sources distributes the absorbed radiation dose over all crystals used for data collection and therefore allows measurement of radiation damage prone systems, including the use of microcrystals for room-temperature measurements. Serial crystallography relies on fast and efficient exchange of crystals upon X-ray exposure, which can be achieved using a variety of methods, including various injection techniques. ...
Tópico(s): Advanced X-ray Imaging Techniques
2017 - International Union of Crystallography | IUCrJ
A. Gorel, Koji Motomura, H. Fukuzawa, R. Bruce Doak, Marie Luise Grünbein, M. Hilpert, Ichiro Inoue, Marco Kloos, G. Kovacsova, Eriko Nango, Karol Nass, C.M. Roome, Robert L. Shoeman, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Makina Yabashi, So Iwata, L. Foucar, Kiyoshi Ueda, Thomas R. M. Barends, Ilme Schlichting,
Abstract Serial femtosecond crystallography at X-ray free-electron lasers (XFELs) offers unprecedented possibilities for macromolecular structure determination of systems prone to radiation damage. However, de novo structure determination, i.e., without prior structural knowledge, is complicated by the inherent inaccuracy of serial femtosecond crystallography data. By its very nature, serial femtosecond crystallography data collection entails shot-to-shot fluctuations in X-ray wavelength and intensity ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2017 - Nature Portfolio | Nature Communications
Jacques‐Philippe Colletier, Michel Sliwa, François-Xavier Gallat, Michihiro Sugahara, Virginia Guillon, Giorgio Schirò, Nicolas Coquelle, Joyce Woodhouse, Laure Roux, Guillaume Gotthard, Antoine Royant, Lucas M. Uriarte, Cyril Ruckebusch, Yasumasa Joti, Martin Byrdin, Eiichi Mizohata, Eriko Nango, Tomoyuki Tanaka, Kensuke Tono, Makina Yabashi, Virgile Adam, Marco Cammarata, Ilme Schlichting, Dominique Bourgeois, Martin H. Weik,
Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown. We employed time-resolved pump-probe absorption spectroscopy on the reversibly photoswitchable fluorescent protein IrisFP in solution to study photoswitching from the nonfluorescent (off) to the fluorescent (on) state. Evidence is provided for the existence of several intermediate states on the pico- and microsecond ...
Tópico(s): Photoreceptor and optogenetics research
2016 - American Chemical Society | The Journal of Physical Chemistry Letters
Karol Nass, Anton Meinhart, Thomas R. M. Barends, L. Foucar, A. Gorel, Andrew Aquila, Sabine Botha, R. Bruce Doak, Jason E. Koglin, Mengning Liang, Robert L. Shoeman, Garth J. Williams, Sébastien Boutet, Ilme Schlichting,
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) offers unprecedented possibilities for macromolecular structure determination of systems that are prone to radiation damage. However, phasing XFEL data de novo is complicated by the inherent inaccuracy of SFX data, and only a few successful examples, mostly based on exceedingly strong anomalous or isomorphous difference signals, have been reported. Here, it is shown that SFX data from thaumatin microcrystals can be successfully ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2016 - International Union of Crystallography | IUCrJ
Tais Gorkhover, Sebastian Schorb, Ryan Coffee, M. Adolph, L. Foucar, Daniela Rupp, Andrew Aquila, John D. Bozek, Sascha W. Epp, Benjamin Erk, Lars Gumprecht, Lotte Holmegaard, Andreas Hartmann, R. Hartmann, Günter Hauser, P. Holl, André Hömke, P. Johnsson, Nils Kimmel, K. U. Kühnel, M. Messerschmidt, Christian Reich, Arnaud Rouzée, Benedikt Rudek, Carlo Schmidt, Joachim Schulz, H. Soltau, Stephan Stern, G. Weidenspointner, Bill White, Jochen Küpper, L. Strüder, Ilme Schlichting, J. Ullrich, Daniel Rolles, Artem Rudenko, T. Möller, Christoph Bostedt,
Tópico(s): Laser-Plasma Interactions and Diagnostics
2016 - Nature Portfolio | Nature Photonics
Onur Dağliyan, Mirosław Tarnawski, Pei-Hsuan Chu, David Shirvanyants, Ilme Schlichting, Nikolay V. Dokholyan, Klaus M. Hahn,
Optogenetic and chemogenetic control of proteins has revealed otherwise inaccessible facets of signaling dynamics. Here, we use light- or ligand-sensitive domains to modulate the structural disorder of diverse proteins, thereby generating robust allosteric switches. Sensory domains were inserted into nonconserved, surface-exposed loops that were tight and identified computationally as allosterically coupled to active sites. Allosteric switches introduced into motility signaling proteins (kinases, ...
Tópico(s): Plant and Biological Electrophysiology Studies
2016 - American Association for the Advancement of Science | Science
Tatiana Domratcheva, Elisabeth Hartmann, Ilme Schlichting, Tilman Kottke,
Abstract BLUF (blue light sensor using flavin) domains regulate the activity of various enzymatic effector domains in bacteria and euglenids. BLUF features a unique photoactivation through restructuring of the hydrogen-bonding network as opposed to a redox reaction or an isomerization of the chromophore. A conserved glutamine residue close to the flavin chromophore plays a central role in the light response, but the underlying modification is still unclear. We labelled this glutamine with 15 N in ...
Tópico(s): Light effects on plants
2016 - Nature Portfolio | Scientific Reports