David Bento, Sara Lopes, Inês M. Gonçalves, Rui Lima, J.M. Miranda,
Air inside of blood vessels is a phenomenon known as gas embolism. During the past years, studies have been performed to assess the influence of air bubbles in microcirculation. In this study, we investigated the flow of bubbles in a microchannel network with several bifurcations, mimicking part of a capillary system. Thus, two working fluids were used, composed by sheep red blood cells (RBCs) suspended in a Dextran 40 solution with different hematocrits (5% and 10%). The experiments were carried ...
Tópico(s): Blood properties and coagulation
2020 - Multidisciplinary Digital Publishing Institute | Micromachines
Maria Inês Teixeira, Carla M. Lopes, Hugo Gonçalves, José Catita, Ana Margarida Silva, Francisca Rodrigues, Maria Helena Amaral, Paulo Costa,
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease, with a median survival of only 2 to 4 years. Riluzole, a drug commonly used in the management of ALS, has a low aqueous solubility and limited bioavailability. ALS treatment is also hindered by the presence of the blood-brain barrier (BBB) that preserves the delicate homeostasis of the cerebral milieu, isolating it and making brain drug delivery exceptionally hard. To overcome these issues, the use of lipid nanocarriers, such ...
Tópico(s): RNA Interference and Gene Delivery
2023 - Elsevier BV | Journal of Molecular Liquids
João–Bruno Soares, Pedro Pimentel‐Nunes, Luís Pedro Afonso, Carla Rolanda, Paula Lopes, Roberto Roncon‐Albuquerque, Nádia Gonçalves, Inês Boal‐Carvalho, Fernando Pardal, Susana Lopes, Guilherme Macedo, Lúcio Lara Santos, Rui Henrique, Luís Moreira‐Dias, Raquel Gonçalves, Mário Dinis‐Ribeiro, Adelino Leite‐Moreira,
We evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-κB, TNF-α, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma group) were consecutively included in the study. Quantification of TLR2, TLR4, NF-κB, TNF-α and ...
Tópico(s): Hepatitis B Virus Studies
2012 - SAGE Publishing | Innate Immunity
Maria Inês Teixeira, Carla M. Lopes, Hugo Gonçalves, José Catita, Ana Margarida Silva, Francisca Rodrigues, Maria Helena Amaral, Paulo Costa,
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with a very poor prognosis. Its treatment is hindered by a lack of new therapeutic alternatives and the existence of the blood–brain barrier (BBB), which restricts the access of drugs commonly used in ALS, such as riluzole, to the brain. To overcome these limitations and increase brain targeting, riluzole-loaded nanostructured lipid carriers (NLC) were prepared and functionalized with lactoferrin (Lf), facilitating transport across the ...
Tópico(s): Lipid Membrane Structure and Behavior
2022 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Rita Cascão, Bruno Vidal, Tânia Carvalho, Inês Lopes, Vasco C. Romão, João Gonçalves, Luís F. Moita, João Eurico Fonseca,
Background: We demonstrated that celastrol has significant anti-inflammatory and bone protective effects when administered via intraperitoneal route. For further preclinical evaluation an effective oral administration of celastrol is crucial. Here we aimed to study the therapeutic dose range for its oral administration. Methods: Celastrol (1-25µg/g/day, N=5/group) was administrated orally to female AIA rats after 8 days of disease induction for a period of 14-days. A group of healthy (N=8) and arthritic ( ...
Tópico(s): Autoimmune and Inflammatory Disorders Research
2020 - Frontiers Media | Frontiers in Medicine
Inês F. G. Reis, Ivo Gonçalves, Marta A. R. Lopes, Carlos Henggeler Antunes,
Collective self-consumption can have an important role contributing to decarbonization and sustainability goals in cities. However, the implementation of such projects is hindered by technical, economic, social and regulatory barriers, which may compromise those goals. Based on the recent guiding principles for collective energy systems established under the scope of the European Union's Clean Energy Package, this work aims to assess how cost minimization and self-consumption maximization collective ...
Tópico(s): Building Energy and Comfort Optimization
2022 - Elsevier BV | Sustainable Cities and Society
Inês F. G. Reis, Ivo Gonçalves, Marta A. R. Lopes, Carlos Henggeler Antunes,
The ‘Clean Energy for All Europeans’ legislative package places citizens and communities at the heart of the European energy policy by promoting local energy generation, consumption and trading. As only recently energy communities were formally defined in the European regulatory framework, the literature on energy community business models is still scattered and a clear systematization of community arrangements is missing. This paper aims to provide a comprehensive view of the prevailing and emergent ...
Tópico(s): Sustainability and Climate Change Governance
2021 - Elsevier BV | Renewable and Sustainable Energy Reviews
Inês F. G. Reis, Ivo Gonçalves, Marta A. R. Lopes, Carlos Henggeler Antunes,
Understanding to what extent the emergence of prosumers and prosumagers organized in energy communities can impact the organization and operation of power grids has been one of the major recent research avenues at the European level. In renewable-based communities aiming to reach some level of energy self-sufficiency, a key issue to be addressed is assessing how the presence of end-users playing different roles in the system (self-consuming, producing and trading, performing demand management, etc.) ...
Tópico(s): Electric Vehicles and Infrastructure
2021 - Multidisciplinary Digital Publishing Institute | Energies
Inês F. G. Reis, Ivo Gonçalves, Marta A. R. Lopes, Carlos Henggeler Antunes,
Energy communities are gaining momentum at the European level as promoters of local and decentralized energy generation, storage, management and trading. Research around these initiatives has been mostly focused on the residential sector. However, communities' self-sufficiency can be further enhanced by synergistically exploiting the demand flexibility of non-residential users. This paper proposes a multiagent system approach in which optimization methods are embedded into the agents’ architecture ...
Tópico(s): Energy Efficiency and Management
2020 - Elsevier BV | Utilities Policy
Tiago Ribeiro, Fernando Ribeiro Gonçalves, Inês S. Garcia, Gil Lopes, António Fernando Ribeiro,
The global population is ageing at an unprecedented rate. With changes in life expectancy across the world, three major issues arise: an increasing proportion of senior citizens; cognitive and physical problems progressively affecting the elderly; and a growing number of single-person households. The available data proves the ever-increasing necessity for efficient elderly care solutions such as healthcare service and assistive robots. Additionally, such robotic solutions provide safe healthcare ...
Tópico(s): Technology Use by Older Adults
2021 - Multidisciplinary Digital Publishing Institute | Applied Sciences
Inês F. G. Reis, Ivo Gonçalves, Marta A. R. Lopes, Carlos Henggeler Antunes,
Local energy trading has the potential to foster the deployment of novel business models especially valuable in citizen-centered self-sufficiency-driven collective energy systems. In this setting, special attention should be paid to vulnerable consumers, who may be neglected in the energy transition due to their limited financial capacity to purchase energy products and services as well as the low energy literacy impairing sound consumption decisions. This paper presents a community-based electricity ...
Tópico(s): Electric Vehicles and Infrastructure
2021 - Elsevier BV | Applied Energy
Cristina Gavina, Inês Falcão‐Pires, J Rodrigues, Benjamim Marinho, Nádia Gonçalves, Ricardo J. Lopes, Mário Jorge Amorim, Jorge Almeida, Paulo Pinho, Alexandra Gonçalves, Francisco Rocha‐Gonçalves, Adelino Leite‐Moreira,
We evaluated the impact of hypertension on the left ventricular mass regression in aortic stenosis after aortic valve replacement.We prospectively studied 135 patients with severe aortic stenosis at baseline and 1 year after surgery. In 32 patients we analyzed myocardial gene expression of collagen types I and III, connective tissue growth factor, transforming growth factor-β1, metalloproteinase-2 and its tissue inhibitor and compared its levels vs controls.Seventy-six patients (56.3%) had a history ...
Tópico(s): Aortic Disease and Treatment Approaches
2013 - Elsevier BV | International Journal of Cardiology
Produção Nacional
Revisado por pares
Maria Carolina Paulino, Catarina Conceição, Joana Silvestre, Maria Inês Lopes, Hernâni Gonçalves, Cláudia Camila Dias, Rodrigo Bernardo Serafim, Jorge I. Salluh, Pedro Póvoa,
Subsyndromal delirium (SSD) in the Intensive Care Unit (ICU) is associated with an increased morbidity with unknown post-discharge functional and cognitive outcomes. We performed a prospective multicenter study to analyze the mental status of patients during their first 72 h after ICU admission and its trajectory, with follow-ups at 3 and 6 months after hospital discharge. Amongst the 106 included patients, SSD occurred in 24.5% (n = 26) and was associated with the duration of mechanical ventilation ( ...
Tópico(s): Healthcare Decision-Making and Restraints
2023 - Multidisciplinary Digital Publishing Institute | Journal of Clinical Medicine
Produção Nacional
Revisado por pares
Luis Guillermo Teherán-Sierra, Michelli Inácio Gonçalves Funnicelli, Lucas Amoroso Lopes de Carvalho, Maria Inês Tiraboschi Ferro, Marcos Antônio Soares, Daniel Guariz Pinheiro,
Plant-associated microbiomes have been a target of interest for the prospection of microorganisms, which may be acting as effectors to increase agricultural productivity. For years, the search for beneficial microorganisms has been carried out from the characterization of functional traits of growth-promotion using tests with a few isolates. However, eventually, the expectations with positive results may not be realized when the evaluation is performed in association with plants. In our study, we ...
Tópico(s): Enzyme Production and Characterization
2021 - Elsevier BV | Microbiological Research
Inês Vitoriano, Kathy D. Saraiva-Pava, Alexandra Rocha‐Gonçalves, Andrea Santos, Ana Isabel Lopes, Mónica Oleastro, Mónica Roxo‐Rosa,
Infection with Helicobacter pylori is the major cause for the development of peptic ulcer disease (PUD). In children, with no other etiology for the disease, this rare event occurs shortly after infection. In these young patients, habits of smoking, diet, consumption of alcohol and non-steroid anti-inflammatory drugs and stress, in addition to the genetic susceptibility of the patient, represent a minor influence. Accordingly, the virulence of the implicated H. pylori strain should play a crucial ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2011 - Public Library of Science | PLoS ONE
Pedro Pimentel‐Nunes, Nádia Gonçalves, Inês Boal‐Carvalho, Luís Pedro Afonso, Paula Lopes, Roberto Roncon‐Albuquerque, Rui Henrique, Luís Moreira‐Dias, Adelino Leite‐Moreira, Mário Dinis‐Ribeiro,
Abstract Background Toll‐like receptors ( TLR ) are essential for H elicobacter pylori ( HP ) recognition. Their role in the progression of gastric lesions leading to cancer is not established. Aim To evaluate for the first time in humans the expression of TLR 2, TLR 4, and TLR 5, as well as the expression of other related molecules in the entire sequence of gastric lesions. Methods Biopsy samples (n = 80, 48% HP +) from normal mucosa, HP gastritis, metaplasia, dysplasia or adenocarcinoma were obtained from 44 ...
Tópico(s): Immune Response and Inflammation
2012 - Wiley | Helicobacter
Pedro Pimentel‐Nunes, Nádia Gonçalves, Inês Boal‐Carvalho, Luís Pedro Afonso, Paula Lopes, Roberto Roncon‐Albuquerque, João–Bruno Soares, Elisabete Cardoso, Rui Henrique, Luís Moreira‐Dias, Mário Dinis‐Ribeiro, Adelino Leite‐Moreira,
Background Animal data suggest that Toll-like receptors (TLR) may play an important role in colon carcinogenesis. Studies in humans to support that hypothesis are scarce. Aim To evaluate the expression of TLR2, TLR4 and TLR5, and the expression of several other related molecules, in different human colonic lesions. Methods Colon biopsy samples from normal mucosa, normal mucosa adjacent to lesion, adenoma or sporadic carcinoma were obtained from 35 consecutive patients undergoing colonoscopy. Quantification ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
2012 - BMJ | Journal of Clinical Pathology
Hugo Gonçalves, Inês Saavedra, Rute A. S. Ferreira, Paulo E. Lopes, Etelvina de Matos Gomes, Michael Belsley,
Intense well polarized second harmonic light was generated by poly(methyl methacrylate) nanofibres with embedded para-nitroaniline nanocrystals. Subwavelength diameter fibres were electro-spun using a 1:2 weight ratio of chromophore to polymer. Analysis of the generated second harmonic light indicates that the para-nitroaniline molecules, which nominally crystalize in the centrosymmetric space group, were organized into noncentrosymmetric structures leading to a second order susceptibility dominated ...
Tópico(s): Gold and Silver Nanoparticles Synthesis and Applications
2018 - Institute of Physics | Journal of Physics D Applied Physics
Marina Fuertes, Joana L. Gonçalves, Anabela Faria, Pedro Lopes dos Santos, Inês C. Conceição, Francisco Dionísio,
Attachment security has been associated with health status and symptom reporting. In this longitudinal study, we investigated the association between antibiotics uptake by infants at 9-months and mother-infant attachment at 12-months. Logistic regression analyses indicated that lower maternal sensitivity was associated with increased odds of antibiotic uptake. Furthermore, 89.7% of insecure-ambivalent infants consumed antibiotics, which contrasted with 32.5% of avoidant infants and 21.5% of secure ...
Tópico(s): Child and Adolescent Psychosocial and Emotional Development
2020 - SAGE Publishing | Journal of Health Psychology
Inês Falcão‐Pires, Nádia Gonçalves, Cosme Moura, Inês Lamego, Catarina Eloy, José Manuel Lopes, Mark P.V. Begieneman, Hans W.M. Niessen, José Carlos Areias, Adelino Leite‐Moreira,
BackgroundStructural and functional changes involved in cardiac injury induced by diabetes mellitus, pressure-overload, or both conditions were evaluated.
Tópico(s): Cardiac electrophysiology and arrhythmias
2009 - Oxford University Press | American Journal of Hypertension
Ana Salomé Pires, Cláudia R. Marques, João Crispim Encarnação, Ana Margarida Abrantes, Inês A. Marques, Mafalda Laranjo, Rui Caetano Oliveira, João Casalta‐Lopes, Ana Cristina Gonçalves, Ana Bela Sarmento‐Ribeiro, Maria Filomena Botelho,
Colorectal cancer (CRC) is continuously classified as one of the most incidental and mortal types of cancer worldwide. The positive outcomes of the conventional chemotherapy are frequently associated with high toxicity, which often leads to the suspension of the treatment. Growing evidences consider the use of pharmacological concentrations of ascorbic acid (AA), better known as vitamin C, in the treatment of cancer. The use of AA in a clinical context is essentially related to the adoption of new ...
Tópico(s): Intestinal and Peritoneal Adhesions
2018 - Frontiers Media | Frontiers in Physiology
Produção Nacional
Revisado por pares
Gevaldo L. de Almeida, Maria Inês Silvani, Rosanne C. A. A. Furieri, Marcelo José Gonçalves, Ricardo Tadeu Lopes,
The divergence of the thermal neutron beam available at the main outlet channel of the Argonauta Reactor in the Instituto de Engenharia Nuclear has been measured by using a Position Sensitive Detector. The measurement process addressing a proper beam characterization has been conducted under several geometric arrangements, including attempts to reduce the beam divergence by using a honeycomb-type gadolinium collimator. Since the detector presents finite resolution, each measured position spectrum ...
Tópico(s): Magnetic confinement fusion research
2005 - Springer Science+Business Media | Brazilian Journal of Physics
Produção Nacional
Ana Xavier‐Magalhães, Céline S. Gonçalves, Anne Fogli, Tatiana Lourenço, Marta Pojo, Bruno Pereira, Miguel Rocha, M Lopes, Inês Crespo, Olinda Rebelo, Herminio Tão, João Lima, Ricardo Moreira, Afonso A. Pinto, Chris Jones, Rui Manuel Reis, Joseph F. Costello, Philippe Arnaúd, Nuno Sousa, Bruno M. Costa,
// Ana Xavier-Magalhães 1, 2, * , Céline S. Gonçalves 1, 2, * , Anne Fogli 3, 4 , Tatiana Lourenço 1, 2 , Marta Pojo 1, 2 , Bruno Pereira 5 , Miguel Rocha 6 , Maria Celeste Lopes 7 , Inês Crespo 7 , Olinda Rebelo 7 , Herminio Tão 7 , João Lima 8 , Ricardo Moreira 8 , Afonso A. Pinto 8 , Chris Jones 9 , Rui M. ...
Tópico(s): RNA Research and Splicing
2018 - Impact Journals LLC | Oncotarget
Lisa Gonçalves, Duarte Gonçalves, Teresa Esteban-Casanelles, Tiago Barroso, Inês Soares de Pinho, Raquel Lopes-Brás, Miguel Esperança‐Martins, Vanessa Pérez, Sofía Torres, Rita Teixeira de Sousa, André Mansinho, Luís Costa,
Although the impact of circadian timing on immunotherapy has yet to be integrated into clinical practice, chronoimmunotherapy is an emerging and promising field as circadian oscillations are observed in immune cell numbers as well as the expression of immunotherapy targets, e.g., programmed cell death protein-1 and its ligand programmed death ligand 1. Concurrent retrospective studies suggest that morning infusions may lead to higher effectiveness of immune checkpoint inhibitors in melanoma, non- ...
Tópico(s): Dietary Effects on Health
2023 - Multidisciplinary Digital Publishing Institute | Cells
Produção Nacional
Revisado por pares
Simone Santiago Carvalho de Oliveira, Diego de Souza Gonçalves, Aline dos Santos Garcia-Gomes, Inês Corrêa Gonçalves, Sérgio Henrique Seabra, Rubem F. S. Menna‐Barreto, Angela H. Lopes, Claudia M. d’Avila-Levy, André Luis Souza dos Santos, Marta H. Branquinha,
A pleiotropic response to the calpain inhibitor MDL28170 was detected in the tomato parasite Phytomonas serpens. Ultrastructural studies revealed that MDL28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans Golgi network. This effect was correlated to the inhibition in processing of cruzipain-like molecules, which presented an increase in expression paralleled by decreased proteolytic activity. Concomitantly, a calcium-dependent cysteine peptidase was detected in ...
Tópico(s): Calpain Protease Function and Regulation
2016 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
João Leote, Tiago Judas, Ana Luísa Broa, Miguel Lopes, Francisca Abecasis, Inês Pintassilgo, Afonso Gonçalves, Filipe Gonzalez,
Lung ultrasound (LUS) is a valuable tool to predict and monitor the COVID-19 pneumonia course. However, the influence of cardiac dysfunction (CD) on LUS findings remains to be studied. Our objective was to determine the effect of CD on LUS in hospitalized patients with COVID-19 pneumonia.Fifty-one patients with COVID-19 pneumonia participated in the study. Focused echocardiography (FoCUS) was carried out on day 1 to separate patients into two groups depending on whether they had FoCUS signs of CD (CD+ ...
Tópico(s): Sepsis Diagnosis and Treatment
2022 - Springer Nature | The Ultrasound Journal
Ana Silva, Estela Vilhena, Alice Lopes, Ernestina Santos, Alexandra Rodrigues Gonçalves, Claudia Mendoza Pinto, Inês Moreira, Denisa Mendonça, Sara Cavaco,
Mood disorders, namely depression and anxiety, have been well documented in patients with Multiple Sclerosis (MS). However, the putative associations between clinical features and mood disorders have not been well established.To detect anxiety and depression in MS patients; and to investigate possible associations with clinical factors.325 consecutive patients with MS and 183 healthy subjects answered the Hospital Anxiety and Depression Scale (HADS), a self-rating questionnaire. Multiple Regression ...
Tópico(s): Long-Term Effects of COVID-19
2011 - Elsevier BV | Journal of the Neurological Sciences
Produção Nacional
Revisado por pares
Simone S. C. Oliveira, Inês Corrêa Gonçalves, Vítor Ennes-Vidal, Angela H. Lopes, Rubem F. S. Menna‐Barreto, Claudia M. d’Avila-Levy, André Luis Souza dos Santos, Marta H. Branquinha,
SUMMARY The species Phytomonas serpens is known to express some molecules displaying similarity to those described in trypanosomatids pathogenic to humans, such as peptidases from Trypanosoma cruzi (cruzipain) and Leishmania spp. (gp63). In this work, a population of P. serpens resistant to the calpain inhibitor MDL28170 at 70 µ m (MDL R population) was selected by culturing promastigotes in increasing concentrations of the drug. The only relevant ultrastructural difference between wild-type (WT) and ...
Tópico(s): Signaling Pathways in Disease
2017 - Cambridge University Press | Parasitology
Filipa Lopes‐Coelho, Sofia Gouveia-Fernandes, Luís G. Gonçalves, Carolina Nunes, Inês Faustino, Fernanda Silva, Ana Félix, Sofia A. Pereira, Jacinta Serpa,
Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, ovarian clear cell carcinoma (OCCC) is intrinsically resistant. The main objective of this study was to explore, in vitro and in vivo, if hepatocyte nuclear factor 1β (HNF1β) and glutaminolysis contribute for the resistance of OCCC to carboplatin through the intrinsically increased GSH bioavailability. To ...
Tópico(s): Sulfur Compounds in Biology
2015 - SAGE Publishing | Tumor Biology
Marlene F. Pereira, Inês M. Amaral, Cátia R. Lopes, Catarina Leitão, Daniela Madeira, João Pedro Lopes, Francisco Q. Gonçalves, Paula M. Canas, Rodrigo A. Cunha, Paula Agostinho,
Increasing evidence shows that astrocytes, by releasing and uptaking neuroactive molecules, regulate synaptic plasticity, considered the neurophysiological basis of memory. This study investigated the impact of l-α-aminoadipate (l-AA) on astrocytes which sense and respond to stimuli at the synaptic level and modulate hippocampal long-term potentiation (LTP) and memory. l-AA selectivity toward astrocytes was proposed in the early 70's and further tested in different systems. Although it has been used ...
Tópico(s): Mitochondrial Function and Pathology
2021 - Wiley | The FASEB Journal