Do Hyun Cho, Hyun‐Jin Kim, Suk Jin Oh, Jeong Hyeon Hwang, Nara Shin, Shashi Kant Bhatia, Jeong‐Jun Yoon, Jong-Min Jeon, Yung‐Hun Yang,
Isobutanol is a potential biofuel, and its microbial production systems have demonstrated promising results. In a microbial system, the isobutanol produced is secreted into the media; however, the ... after fermentation cannot be used efficiently during the isobutanol recovery process and are discarded as waste. To ... of utilizing these remaining cells by combining the isobutanol production system with the indigo production system, wherein ... ilvD), and alpha-ketoisovalerate decarboxylase gene (kivD), for isobutanol production and genes, such as tryptophanase gene (tnaA) ...
Tópico(s): Biofuel production and bioconversion
2023 - Elsevier BV | Journal of Biotechnology
Hao Xie, Jarl Kjellström, Peter Lindblad,
... sp. PCC 6803 has been engineered for the isobutanol and 3-methyl-1-butanol production by introducing ... Synechocystis sp. PCC 6803 for further enhanced photosynthetic isobutanol and 3-methyl-1-butanol production.Long-term ... on two selected strains resulting in maximum cumulative isobutanol and 3-methyl-1-butanol titers of 1247 ... generated and showed a production of 98 mg isobutanol L-1 in short-term screening experiments. Enhanced isobutanol and 3-methyl-1-butanol production was observed ... the kivdS286T copy number from three to four. Isobutanol and 3-methyl-1-butanol production was effectively ...
Tópico(s): Photosynthetic Processes and Mechanisms
2023 - BioMed Central | Biotechnology for Biofuels and Bioproducts
Aneesh Vijay Kale, Anand Krishnasamy,
... in port fuel injected HCCI mode with gasoline-isobutanol blends. All the experiments were performed at a ... and emission reduction were examined. The use of isobutanol, a gasoline-like high volatile, low reactivity, renewable ... at lower engine loads. The volumetric proportion of isobutanol in the fuel blends was increased from 20% ... HCCI to 70% (of rated engine load) for isobutanol-gasoline-2-EHN-fuelled HCCI. The indicated thermal efficiency improved with increased isobutanol in the fuel blends for a particular engine ...
Tópico(s): Biodiesel Production and Applications
2022 - Elsevier BV | Energy Conversion and Management
Francesca V. Gambacorta, Ellen R. Wagner, Tyler B. Jacobson, Mary Tremaine, Laura K. Muehlbauer, Mick McGee, Justin J. Baerwald, Russell L. Wrobel, John F. Wolters, Mike Place, Joshua J. Dietrich, Dan Xie, Jose Serate, Shabda Gajbhiye, Lisa Liu, Maikayeng Vang-Smith, Joshua J. Coon, Yaoping Zhang, Audrey P. Gasch, Daniel Amador‐Noguez, Chris Todd Hittinger, Trey K. Sato, Brian F. Pfleger,
... been successfully implemented to improve the production of isobutanol, a next-generation biofuel, in Saccharomyces cerevisiae. Here, ... cofactor-balancing, affect the performance and physiology of isobutanol producing strains. We equipped yeast with isobutanol cassettes which had either a mitochondrial or cytosolic localized isobutanol pathway and used either a redox-imbalanced (NADPH- ... strains. Pathway localization had a large effect on isobutanol production with the strain expressing the mitochondrial-localized ...
Tópico(s): Fungal and yeast genetics research
2022 - Elsevier BV | Synthetic and Systems Biotechnology
Xiaoxi Wu, Jianwei Li, Su‐Fang Xing, Huiting Chen, Chao Song, Shuguang Wang, Zhen Yan,
Abstract Background Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO 2 into bio-based fuels ... Although autotrophic cyanobacteria have been genetically modified for isobutanol biosynthesis, there is a lack of stable and ... this study, we first engineered Synechococcus elongatus for isobutanol biosynthesis by introducing five exogenous enzymes, reaching a ... could result in a whopping fivefold increase in isobutanol production, with a maximal in-flask titer of ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2021 - Springer Science+Business Media | Biotechnology for Biofuels
Yangyang Zhan, Yong Xu, Xingcheng Lu, Fei Zhou, Pengling Zheng, Dong Wang, Dongbo Cai, Shihui Yang, Shouwen Chen,
Isobutanol is regarded as a renewable alternative to fossil fuels. In this study, Bacillus licheniformis DWc9n was ... grow in the presence of 16 g/L isobutanol, showing high resistance to isobutanol. To achieve high-level production of isobutanol, a push–pull–restrain strategy was employed. First, ... dehydrogenase YugJ with the highest synthesis efficiency of isobutanol was identified by comparing different heterologous and endogenous ... competing pathways resulted in a remarkable increase in isobutanol production. Moreover, gene bcd encoding leucine dehydrogenase was ...
Tópico(s): Enzyme Catalysis and Immobilization
2021 - American Chemical Society | ACS Sustainable Chemistry & Engineering
Hsien-Lin Liu, Christine H.-T. Wang, En‐Pei Isabel Chiang, Chieh‐Chen Huang, Wen‐Hsiung Li,
Isobutanol is considered a potential biofuel, thanks to its high-energy content and octane value, limited water ... microorganism, such as Saccharomyces cerevisiae, that inherently produces isobutanol, can serve as a good engineering host. Isobutanol's toxicity, however, is a major obstacle for ... This study is to understand how yeast tolerates isobutanol. A S. cerevisiae gene-deletion library with 5006 mutants was used to screen genes related to isobutanol tolerance. Image recognition was efficiently used for high- ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2021 - Springer Science+Business Media | Biotechnology for Biofuels
Miguel G. Acedos, Isabel de la Torre, Victoria E. Santos, Félix Garcı́a-Ochoa, José L. Garcı́a, Beatriz Galán,
Abstract Background Isobutanol is a candidate to replace gasoline from fossil resources. This higher alcohol can be produced from sugars using ... a robust strain resistant to high concentration of isobutanol that can achieve a high production rate of ... Nevertheless, this strain, like most strains developed for isobutanol production, has some limitations in its metabolic pathway. Isobutanol production under anaerobic conditions leads to a depletion ... blattae (p424IbPSO) resulted in a 19.3% higher isobutanol production. The recombinant strain S. blattae (p424IbPSO, pIZpntAB) ...
Tópico(s): Enzyme Catalysis and Immobilization
2021 - Springer Science+Business Media | Biotechnology for Biofuels
Sandra Weitz, Maria Hermann, Sonja Linder, Frank R. Bengelsdorf, Ralf Takors, Peter Dürre,
Two different isobutanol synthesis pathways were cloned into and expressed in the two model acetogenic bacteria Acetobacterium woodii and Clostridium ljungdahlii . ... Only in recombinant A. woodii strains, traces of isobutanol could be detected. Additional feeding of ketoisovalerate increased isobutanol production to 2.9 mM under heterotrophic conditions ... autotrophic conditions using kor 2. In C. ljungdahlii , isobutanol could only be detected upon additional ketoisovalerate feeding ... heterotrophic conditions, A. woodii produced 0.2 mM isobutanol and 0.4 mM upon additional ketoisovalerate feeding. ...
Tópico(s): Enzyme Catalysis and Immobilization
2021 - Frontiers Media | Frontiers in Bioengineering and Biotechnology
Yide Su, Wenju Shao, Aili Zhang, Wei Zhang,
ABSTRACT Improving yeast tolerance toward isobutanol is a critical issue enabling high-titer industrial production. Here, we used EMS mutagenesis to screen Saccharomyces cerevisiae with greater tolerance toward isobutanol. By this method, we obtained EMS39 with high-viability in medium containing 16 g/L isobutanol. Then, we metabolically engineered isobutanol synthesis in EMS39. About 2μ plasmids carrying PGK1p- ... 20. Our results showed that EMS39-20 increased isobutanol titers by 49.9% compared to W303-1A- ...
Tópico(s): Fermentation and Sensory Analysis
2021 - Oxford University Press | FEMS Yeast Research
Chuhan Fu, Zhuoxi Li, Yulei Zhang, Conghua Yi, Shaoqu Xie,
Abstract Isobutanol is a widely used platform compound and a raw material for synthesizing many high value‐added compounds. ... or substitute with a very broad development space. Isobutanol production by biological fermentation has the advantages of ... also has disadvantages such as many impurities, low isobutanol concentration, and difficulty separating the water + isobutanol azeotrope. Thus, it is necessary to explore an appropriate downstream separation process for the water + isobutanol azeotrope. K 2 CO 3 with a strong ...
Tópico(s): Extraction and Separation Processes
2021 - Wiley | Engineering in Life Sciences
Chuhan Fu, Zhuoxi Li, Chuhua Jia, Wanli Zhang, Yulei Zhang, Conghua Yi, Shaoqu Xie,
Bio-based isobutanol has attracted more and more attention due to its wide application and excellent fuel performance. Compared with ethanol, isobutanol has higher energy density, lower oxygen content, and ... an ideal gasoline additive or substitute. Bio-based isobutanol has been commercially produced from a variety of ... ethanol plants is acquired to separate and purify isobutanol from the fermentation broth, meaning that the energy- ... some challenges in the production of bio-based isobutanol, such as low product concentration, unexpected impurities, high ...
Tópico(s): Catalysis for Biomass Conversion
2020 - Elsevier BV | Energy Conversion and Management X
Mengyue Qiu, Wei Shen, Xiongyin Yan, Qiaoning He, Dongbo Cai, Shouwen Chen, Hui Wei, Eric P. Knoshaug, Min Zhang, Michael E. Himmel, Shihui Yang,
... to meet global sustainable energy and environmental goals. Isobutanol is a four-carbon alcohol with many advantages ... biorefinery. Results In this study, the effect of isobutanol on Z. mobilis was investigated, and various isobutanol-producing recombinant strains were constructed. The results showed ... was able to grow in the presence of isobutanol below 12 g/L while concentrations greater than ... as kdcA from Lactococcus lactis is required for isobutanol production in Z. mobilis . Moreover, isobutanol production increased ...
Tópico(s): Enzyme Catalysis and Immobilization
2020 - Springer Science+Business Media | Biotechnology for Biofuels
Satoshi Hasegawa, Toru Jojima, Masako Suda, Masayuki Inui,
... L-valine production under oxygen deprivation, we developed isobutanol-producing Corynebacterium glutamicum strains. The artificial isobutanol synthesis pathway was composed of the first three ... was decarboxylated into isobutyraldehyde, and subsequently reduced into isobutanol in the latter reactions. Although there exists redox ... via glycolysis whereas NADPH is required to synthesize isobutanol, it was resolved by taking advantage of the ... encoded by adhA. Each enzyme activity to synthesize isobutanol was finely tuned by using two kinds of ...
Tópico(s): Enzyme Catalysis and Immobilization
2020 - Elsevier BV | Metabolic Engineering
Johannes Wess, Martin Brinek, Eckhard Boles,
Isobutanol is a promising candidate as second-generation biofuel and has several advantages compared to bioethanol. Another benefit of isobutanol is that it is already formed as a ... Saccharomyces cerevisiae, although only in very small amounts. Isobutanol formation results from valine degradation in the cytosol ... is one of the limiting factors for higher isobutanol production in yeast. Furthermore, some intermediate metabolites are also substrates for various isobutanol competing pathways, reducing the metabolic flux toward isobutanol ...
Tópico(s): Fermentation and Sensory Analysis
2019 - Springer Science+Business Media | Biotechnology for Biofuels
Huan Yu, Ning Wang, Wenbo Huo, Yuhong Zhang, Wei Zhang, Yu Yang, Zhenya Chen, Yi‐Xin Huo,
Isobutanol, a C4 branched-chain higher alcohol, is regarded as an attractive next-generation transport fuel. Metabolic engineering for efficient isobutanol production has been achieved in many studies. BmoR, ... study, to achieve the high-level production of isobutanol, we established a screening system which relied on the combination of BmoR-based biosensor and isobutanol biosynthetic pathway and then employed it to screen isobutanol overproduction strains from an ARTP mutagenesis library. Firstly, ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2019 - BioMed Central | Microbial Cell Factories
Rui Miao, Hao Xie, Peter Lindblad,
... direct processes. The concept of photosynthetic production of isobutanol, a promising chemical and drop-in biofuel, has ... decarboxylase (Kivd) from Lactococcus lactis resulted in an isobutanol and 3-methyl-1-butanol producing strain. Kivd ... replacement of serine-286 to threonine (KivdS286T). However, isobutanol production still remained low. In the present study, ... report on how cultivation conditions significantly affect the isobutanol production in Synechocystis PCC 6803. A HCl-titrated ... photons m−2 s−1) showed the highest isobutanol production with an in-flask titer of 194 ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2018 - Springer Science+Business Media | Biotechnology for Biofuels
Shaoxiong Liang, Hong Chen, Jiao Liu, Jianping Wen,
Abstract Isobutanol as a more desirable biofuel has attracted much attention. In our previous work, an isobutanol-producing strain Escherichia coli LA09 had been obtained ... model. However, the low transformation from sugar to isobutanol is a limiting factor for isobutanol production by E. coli LA09. In this study, the intracellular metabolic profiles of the isobutanol-producing E. coli LA09 with different initial glucose ... deoxy-6-phospho-gluconate (KDPG). Finally, the best isobutanol-producing E. coli ED02 with higher glucose transformation ...
Tópico(s): Enzyme Catalysis and Immobilization
2018 - Springer Science+Business Media | Journal of Industrial Microbiology & Biotechnology
Sarah K. Hammer, José L. Avalos,
Isobutanol and other branched-chain higher alcohols (BCHAs) are promising advanced biofuels derived from the degradation of ... However, the roles that these transaminases play in isobutanol production remain poorly understood and obscured by conflicting ... work, we elucidate the influence of BCATs on isobutanol production in two genetic backgrounds (CEN.PK2-1C ... the process, we uncover and characterize two competing isobutanol pathways, which can be manipulated by overexpressing or ... We show that deletion of BAT1 alone increases isobutanol production by 14.2-fold over wild type ...
Tópico(s): Fermentation and Sensory Analysis
2017 - Elsevier BV | Metabolic Engineering
Jinjie Gu, Jidong Zhou, Zhongxi Zhang, Cheorl‐Ho Kim, Biao Jiang, Jiping Shi, Jian Hao,
Isobutanol is a valuable chemical and is considered a new generation biofuel. Construction of isobutanol synthesis pathways in bacteria is a hot topic in isobutanol production. Here, we show that an isobutanol synthesis pathway exists naturally in Klebsiella pneumoniae; however, ... synthesis pathways of 2,3-butanediol, valine and isobutanol all start from condensation of two pyruvate molecules ... the valine pathway, which led to synthesis of isobutanol and 2-ketoisovalerate (a precursor of isobutanol). ldhA ( ...
Tópico(s): Enzyme Catalysis and Immobilization
2017 - Elsevier BV | Metabolic Engineering
Rui Miao, Xufeng Liu, Elias Englund, Pia Lindberg, Peter Lindblad,
Isobutanol is a flammable compound that can be used as a biofuel due to its high energy ... different heterologous and endogenous alcohol dehydrogenases (ADH) for isobutanol production. A strain expressing an introduced kivd without ... ADH produced 3 mg L-1 OD750-1 isobutanol in 6 days. After the cultures were supplemented ... substrate for ADH, 60.8 mg L-1 isobutanol was produced after 24 h when OD750 was ... encoded by slr1192 showed the highest efficiency for isobutanol production. Furthermore, the strain overexpressing the isobutanol pathway ...
Tópico(s): Biofuel production and bioconversion
2017 - Elsevier BV | Metabolic Engineering Communications
N. Milne, A. Wahl, Antonius J. A. van Maris, Jack T. Pronk, Jean‐Marc Daran,
... possible to engineer Saccharomyces cerevisiae strains in which isobutanol is the predominant catabolic product and high-yielding isobutanol-producing strains are already reported by industry. Conversely, isobutanol yields of engineered S. cerevisiae strains reported in ... mass-balancing approach. A cytosolically located, cofactor-balanced isobutanol pathway, consisting of a mosaic of bacterial enzymes ... was oxidized to isobutyrate rather than reduced to isobutanol. Moreover, significant concentrations of the pathway intermediates 2, ...
Tópico(s): Biofuel production and bioconversion
2016 - Elsevier BV | Metabolic Engineering Communications
Aili Zhang, Yang Li, Yuhan Gao, Jin Hongxing,
As a new biofuel, isobutanol has received more attentions in recent years. Because of its high tolerance to higher alcohols, Saccharomyces cerevisiae has potential advantages as a platform microbe to produce isobutanol. In this study, we investigated integration effects of ... acetyl-CoA biosynthesis by deletion of LPD1 on isobutanol titers. Our results showed that deletion of LPD1 in strains overexpressing BAT2 and ILV2 increased isobutanol titer by 5.3-fold compared with control ... strains carrying overexpressed BAT2 and ILV2 further increased isobutanol titer by 1.5 fold. Overexpression of BAT2 ...
Tópico(s): Fungal and yeast genetics research
2016 - Elsevier BV | Chinese Journal of Chemical Engineering
Jiao Liu, Haishan Qi, Cheng Wang, Jianping Wen,
Few strains have been found to produce isobutanol naturally. For building a high performance isobutanol-producing strain, rebalancing redox status of the cell was very crucial through systematic investigation of redox ... modulation of the redox status.Firstly, a starting isobutanol-producing E. coli strain LA02 was engineered with only 2.7 g/L isobutanol produced. Then, the genome-scale metabolic modeling was ... and 6.08 g/L, respectively. Therefore, the isobutanol titer was increased by 221% to 8.68 ...
Tópico(s): Gene Regulatory Network Analysis
2015 - Springer Science+Business Media | Biotechnology for Biofuels
Kengo Ida, Jun Ishii, Fumio Matsuda, Takashi Kondo, Akihiko Kondo,
Isobutanol is an important biorefinery target alcohol that can be used as a fuel, fuel additive, or ... a promising organism for the industrial manufacture of isobutanol because of its tolerance for low pH and ... which is directly involved in pyruvate metabolism, improved isobutanol production by S. cerevisiae. However, the engineering strategies ... pathways in addition to pyruvate metabolism compete with isobutanol production. The isobutyrate, pantothenate or isoleucine biosynthetic pathways ... to reduce the outflow of carbon competing with isobutanol biosynthesis in S. cerevisiae. The judicious elimination of ...
Tópico(s): Fungal and yeast genetics research
2015 - BioMed Central | Microbial Cell Factories
Haishan Qi, Shanshan Li, Sumin Zhao, Di Huang, Menglei Xia, Jianping Wen,
To rationally guide the improvement of isobutanol production, metabolic network and metabolic profiling analysis were performed to provide global and profound insights into cell metabolism of isobutanol-producing Bacillus subtilis. The metabolic flux distribution of strains with different isobutanol production capacity (BSUL03, BSUL04 and BSUL05) drops a hint of the importance of NADPH on isobutanol biosynthesis. Therefore, the redox pathways were redesigned in ... that in parental strain BSUL05. However, cell growth, isobutanol yield and production were decreased by 46%, 22%, ...
Tópico(s): Biofuel production and bioconversion
2014 - Public Library of Science | PLoS ONE
Fumio Matsuda, Jun Ishii, Takashi Kondo, Kengo Ida, Hironori Tezuka, Akihiko Kondo,
Isobutanol is an important target for biorefinery research as a next-generation biofuel and a building block ... chemical production. Metabolically engineered microbial strains to produce isobutanol have been successfully developed by introducing the Ehrlich pathway into bacterial hosts. Isobutanol-producing baker's yeast (Saccharomyces cerevisiae) strains have ... respect to its advantageous characteristics for cost-effective isobutanol production. However, the isobutanol yields and titers attained by the developed strains ...
Tópico(s): Fungal and yeast genetics research
2013 - BioMed Central | Microbial Cell Factories
Ling Tao, Eric C. D. Tan, Robert L. McCormick, Min Zhang, Andy Aden, Xin He, Bradley T. Zigler,
... the production design and economics of the cellulosic isobutanol conversion processes and compares cellulosic isobutanol with cellulosic ethanol and n‐butanol in the ... is used to understand the current stage of isobutanol process development and the impact of key parameters ... plant scale, and cost basis). The calculated minimum isobutanol selling price is $3.62/gasoline gallon equivalent ($/ ... 2 , at 26.42 kg CO 2 / GGE . Isobutanol and ethanol plants have relatively similar CO 2 ... the following order: ethanol (8.19 gal/ GGE ) < isobutanol (8.98 gal/ GGE ) < n‐butanol (10.84 ...
Tópico(s): Catalysis for Biomass Conversion
2013 - Wiley | Biofuels Bioproducts and Biorefining
Huiqing Chong, Hefang Geng, Hongfang Zhang, Hao Song, Lei Huang, Rongrong Jiang,
ABSTRACT The limited isobutanol tolerance of Escherichia coli is a major drawback during fermentative isobutanol production. Different from classical strain engineering approaches, this work was initiated to improve E. coli isobutanol tolerance from its transcriptional level by engineering its ... of crp , and the libraries were subjected to isobutanol stress for selection. Variant IB2 (S179P, H199R) was ... 05 h −1 ) in 1.2% ( v/v ) isobutanol (9.6 g/L). Genome‐wide DNA microarray ... the absence and presence of 1% ( v/v ) isobutanol, respectively. When challenged with isobutanol, genes related to ...
Tópico(s): Bacterial Genetics and Biotechnology
2013 - Wiley | Biotechnology and Bioengineering
Arul M. Varman, Yi Xiao, Himadri B. Pakrasi, Yinjie Tang,
... 6803 to develop a strain that can synthesize isobutanol under both autotrophic and mixotrophic conditions. With the ... engineered strain can accumulate 90 mg/liter of isobutanol from 50 mM bicarbonate in a gas-tight ... 1-thiogalactopyranoside [IPTG]) or antibiotics to maintain its isobutanol production. In the presence of glucose, isobutanol synthesis is only moderately promoted (titer = 114 mg/ ... mainly employed autotrophic metabolism for biomass growth and isobutanol production. Since isobutanol is toxic to the cells ...
Tópico(s): Photosynthetic Processes and Mechanisms
2012 - American Society for Microbiology | Applied and Environmental Microbiology