Mihailo Bezmarević, Darko Mirković, Ivan Soldatović, Dušica Stamenković, Nikola Mitrović, Nenad Perišić, Ivan Marjanović, Saša Micković, Μενέλαος Καρανικόλας,
Early assessment of disease severity and vigilant patient monitoring are key factors for adequate treatment of acute pancreatitis (AP). The aim of this study was to determine the correlation of procalcitonin (PCT) serum concentrations and intra-abdominal pressure (IAP) as prognostic markers in early stages of AP. This prospective observational study included 51 patients, of which 29 had severe AP (SAP). Patients were evaluated with the Acute Physiology And Chronic Health Evaluation (APACHE II) score, ...
Tópico(s): Hernia repair and management
2012 - Elsevier BV | Pancreatology
Gordana Leposavić, Vesna Pešić, Zorica Stojić-Vukanić, Katarina Radojević, Nevena Arsenović-Ranin, Duško Kosec, Milica Perišić Nanut, Ivan Pilipović,
Alpha(1)-adrenoceptors (α(1)-ARs) are involved in neuro-thymic and thymic intercellular communications, and consequently modulation of T-cell development. Ageing is associated with a number of changes in noradrenergic neuro-effector transmission, and possibly intercellular noradrenaline (NA)-mediated communication resulting in altered responses of target cells to NA. Thus, in old animals an altered NA modulation of thymopoiesis via α(1)-ARs may be expected. To test this hypothesis, in old and young ...
Tópico(s): Tryptophan and brain disorders
2010 - Elsevier BV | Experimental Gerontology
Dušan Mijin, Gordana Uščumlić, Nada Perišić‐Janjić, Ivan Trkulja, Maja Radetić, Petar Jovanĉić,
A series of 5-(3- and 4-substituted phenylazo)-4,6-dimethyl-3-cyano-2-pyridones were synthesized, starting from acetylacetone and arenediazonium salt, followed by condensation with cyanoacetamide using a modified literature procedure. The experimental investigation included modification of the synthetic procedure in terms of solvent, temperature, isolation techniques as well as purification and identification of the products. The azo dyes were characterized by melting point, IR, UV/Vis, 1H NMR and ...
Tópico(s): Synthesis and biological activity
2006 - Serbian Chemical Society | Journal of the Serbian Chemical Society
V. Pesic, Katarina Radojević, Duško Kosec, Bosiljka Plećaš‐Solarović, Milica Perišić Nanut, Ivan Pilipović, Gordana Leposavić,
The role of gonadal hormones in induction and, particularly, maintenance/progression of rat thymic involution, which normally starts around puberty, was reassessed by examining the effects of peripubertal orchidectomy on thymic weight and morphometric parameters at different times up to the age of 10 months. Up to 6 months post-castration both thymic weight and cellularity in orchidectomized (Cx) rats were greater than in age-matched control rats, sham Cx (Sx). The increase in thymic cellularity reflected ...
Tópico(s): Hypothalamic control of reproductive hormones
2007 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Gordana Leposavić, Milica Perišić Nanut, Ivan Pilipović,
Tópico(s): Hormonal and reproductive studies
2012 - Springer Science+Business Media | Immunologic Research
Milica Perišić Nanut, Nevena Arsenović-Ranin, Ivan Pilipović, Duško Kosec, Vesna Pešić, Katarina Radojević, Gordana Leposavić,
A commonly held view that ovarian hormones are causally involved in age-associated thymic involution has been recently challenged. In particular, their relevance in the progression of thymic involution has been disputed. To reassess this issue 10-month-old rats with well advanced thymic involutive changes were ovariectomized (Ovx), and after 1 month thymic cellularity, thymocyte development and levels of recent thymic emigrants (RTEs) were examined in peripheral blood and spleen. In addition, the ...
Tópico(s): Lymphatic System and Diseases
2009 - Elsevier BV | Immunobiology
Katarina Radojević, Duško Kosec, Milica Perišić Nanut, Ivan Pilipović, Biljana Vidić‐Danković, Gordana Leposavić,
Abstract We tested the hypothesis that neonatal androgenization affects the efficacy of β-adrenoceptor (β-AR)-mediated fine tuning of thymopoiesis in adult female rats by modulating the thymic noradrenaline (NA) level and/or β-AR expression. In adult rats administered with 1000μg testosterone enanthate at postnatal day 2 a higher density of catecholamine (CA)-synthesizing thymic cells, including thymocytes, and a rise in their CA content was found. In addition, in these animals increased thymic noradrenergic ...
Tópico(s): Adrenal Hormones and Disorders
2011 - Elsevier BV | Journal of Neuroimmunology
Milica Perišić Nanut, Zorica Stojić-Vukanić, Ivan Pilipović, Duško Kosec, Mirjana Nacka-Aleksić, Jasmina Đikić, Nevena Arsenović-Ranin, Gordana Leposavić,
The study explored the putative role of ovarian hormones in the peripubertal remodelling of peripheral T-cell compartment. Ovariectomy at age of 1 month enhanced the peripubertal rise in CD4+ and CD8+ cell numbers in peripheral blood (PB) and spleen from 2-month-old rats. This reflected maintenance of thymopoietic efficiency at the prepubertal level (judging by numbers of the most mature CD4+ and CD8+ thymocytes and recent thymic emigrants) and alterations in T-cell survival/proliferation in the periphery. ...
Tópico(s): Reproductive System and Pregnancy
2012 - Elsevier BV | Immunobiology
Zorica Stojić-Vukanić, Biljana Bufan, Nevena Arsenović-Ranin, Duško Kosec, Ivan Pilipović, Milica Perišić Nanut, Gordana Leposavić,
Tópico(s): Immune Response and Inflammation
2013 - Springer Science+Business Media | Biogerontology
Gordana Leposavić, Ivan Pilipović, Milica Perišić Nanut,
The thymus plays a critical role in establishing and maintaining the peripheral T-cell pool. It does so by providing a microenvironment within which T-cell precursors differentiate and undergo selection processes to create a functional population of major histocompatibility complex-restricted, self-tolerant T cells. These cells are central to adaptive immunity. Thymic T-cell development is influenced by locally produced soluble factors and cell-to-cell interactions, as well as by sympathetic noradrenergic ...
Tópico(s): T-cell and B-cell Immunology
2011 - Institute of Physiology of the Czech Academy of Sciences | Physiological Research
Vesna Pešić, Duško Kosec, Katarina Radojević, Ivan Pilipović, Milica Perišić Nanut, Biljana Vidić‐Danković, Gordana Leposavić,
The study was undertaken to explore: i) the presence of alpha(1)-adrenoceptors (AR) on thymic lymphoid and non-lymphoid cells and ii) their putative role in T-cell development. The expression of alpha(1)-AR on thymic cells was assessed using both immunohistochemistry and flow cytometric analyses, while their putative role in thymopoiesis was estimated by analyses of thymocyte proliferation and apoptosis, and major thymocyte subset distribution in adult rats subjected to 14-day-long treatment with ...
Tópico(s): T-cell and B-cell Immunology
2009 - Elsevier BV | Journal of Neuroimmunology
Katarina Radojević, N Arsenović-Ranin, Duško Kosec, Vesna Pešić, Ivan Pilipović, Milica Perišić Nanut, Bosiljka Plećaš‐Solarović, Gordana Leposavić,
To test putative interdependence in the ontogenesis of the hypothalamic–pituitary–gonadal and thymic–lymphatic axes, thymocyte differentiation and maturation was examined in neonatally castrated (Cx) adult rats. In the hypercellular thymi of Cx rats, the proportion of the least mature CD4 − CD8 − TCRαβ − triple negative (TN) thymocytes was reduced, while the proportions of all downstream double positive (DP) subsets (TCRαβ − , TCRαβ low and TCRαβ high ) were increased when compared with neonatally sham-castrated ( ...
Tópico(s): Diabetes and associated disorders
2007 - Bioscientifica | Journal of Endocrinology
Vesna Pešić, Bosiljka Plećaš‐Solarović, Katarina Radojević, Duško Kosec, Ivan Pilipović, Milica Perišić Nanut, Gordana Leposavić,
Age-related increase in the density of thymic noradrenergic fibres and noradrenaline (NA) concentration is proposed to be associated with thymic involution and altered thymopoiesis. To test this hypothesis thymocyte differentiation/maturation and thymic structure were studied in 18-month-old male Wistar rats subjected to 14-day-long propranolol (P) blockade of beta-adrenoceptors (beta-ARs). The treatment primarily resulted in changes in the T-cell receptor (TCR)-dependent stages of thymopoiesis, which ...
Tópico(s): Stress Responses and Cortisol
2007 - Elsevier BV | International Immunopharmacology
Gordana Leposavić, Vesna Pešić, Duško Kosec, Katarina Radojević, Nevena Arsenović-Ranin, Ivan Pilipović, Milica Perišić Nanut, Bosiljka Plećaš‐Solarović,
To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCRαβ and (ii) Thy-1, which is supposed to be a regulator of TCRαβ signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCRαβ signalling in the development of CD4+25+Treg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double ...
Tópico(s): Immunotherapy and Immune Responses
2006 - Elsevier BV | Experimental Gerontology
Gordana Leposavić, Katarina Radojević, Biljana Vidić‐Danković, Duško Kosec, Ivan Pilipović, Milica Perišić Nanut,
The interactions among the nervous, endocrine and immune system were studied by examining: i) thymic and thymocyte catecholamine levels in adult rats castrated (Cx) at postnatal day 3 and ii) effects of 14-day-long propranolol (P) treatment on main thymocyte differentiational molecule expression in adult non-Cx and Cx rat. The results demonstrated that castration in early postnatal period lowers levels of both neurally- and thymocyte-derived noradrenaline in adult rats, and thereby diminishes beta- ...
Tópico(s): Neuroendocrine regulation and behavior
2006 - Elsevier BV | Journal of Neuroimmunology
Gordana Leposavić, Milica Perišić Nanut, Ivan Pilipović, Duško Kosec, Nevena Arsenović-Ranin, Zorica Stojić-Vukanić, Jasmina Djikić, Mirjana Nacka-Aleksić,
This study explores the role of ovarian hormones in the phenotypic shaping of peripheral T-cell pool over the reproductive lifespan of rats. For this purpose, 2-month-old prepubertally ovariectomised (Ox) rats, showing oestrogen and progesterone deficiency, and 11-month-old Ox rats, exhibiting only progesterone deficiency, were examined for thymus output, and cellularity and composition of major TCRαβ+ peripheral blood lymphocyte (PBL) and splenocyte subsets. Although ovariectomy increased thymic ...
Tópico(s): Stress Responses and Cortisol
2013 - Elsevier BV | Immunobiology
Vojislav N. Perisic, Emma Howard, T. Mihailovic, Gordan Vujanić, Dragan Milovanović, Ivan Ivanovski,
A 4-year-old boy with a short history of jaundice and hepatomegaly was studied by endoscopic retrograde cholangiopancreatography (ERCP) and percutaneous transhepatic cholangiography (PTC). A large obstructing choledochal tumour was found at the level of cystic duct insertion and at surgery, a botryoid sarcoma was removed. Subsequent cytotoxic therapy was complicated by persistent dilatation of the extrahepatic bile ducts which necessitated eventual excision. There was no evidence of persistent tumour.
Tópico(s): Genetic and Kidney Cyst Diseases
1991 - Thieme Medical Publishers (Germany) | European Journal of Pediatric Surgery
Gordana Leposavić, Ivan Pilipović, Milica Perišić Nanut,
Ageing is associated with a progressive decline in thymic cytoarchitecture followed by a less efficient T cell development and decreased emigration of naïve T cells to the periphery. These thymic changes are linked to increased morbidity and mortality from infectious, malignant and autoimmune diseases in old age. Therefore, it is of paramount importance to understand the thymic homeostatic processes across the life span, as well as to identify factors and elucidate mechanisms driving or contributing ...
Tópico(s): T-cell and B-cell Immunology
2011 - Karger Publishers | NeuroImmunoModulation
Gordana Leposavić, Milica Perišić Nanut, Duško Kosec, Nevena Arsenović-Ranin, Katarina Radojević, Zorica Stojić-Vukanić, Ivan Pilipović,
Exposure of female rodents to testosterone in the critical neonatal period produces defeminization/masculinization of the hypothalamo–pituitary–gonadal (HPG) axis, i.e. neonatal androgenization and postpones axis maturation. To address the hypothesis that HPG axis signaling is involved in the programming of thymic maturation/involution and sexual differentiation we studied the impact of neonatal androgenization on thymic cellularity, development of effector and regulatory T cells, and phenotypic ...
Tópico(s): Circadian rhythm and melatonin
2008 - Elsevier BV | Brain Behavior and Immunity
Ivan Pilipović, Biljana Vidić‐Danković, Milica Perišić Nanut, Katarina Radojević, Miodrag Čolić, Vera Todorović, Gordana Leposavić,
The study was undertaken to explore whether there were: i) apart from neural and circulatory, some other sources of catecholamines (CAs) in rat thymus and ii) gender-specific differences in thymic CA levels, and if so to elucidate the role of sex steroids in this phenomenon. Tyrosine hydroxylase (TH) immunoreactivity was found in thymocytes and thymic epithelial cells (some of which showed morphological features of nurse cells). The density of CA-synthesizing cells was greater in male than in female ...
Tópico(s): Estrogen and related hormone effects
2008 - Elsevier BV | Journal of Neuroimmunology
Gordana Leposavić, Ivan Pilipović, Katarina Radojević, Vesna Pešić, Milica Perišić Nanut, Duško Kosec,
In its simplest form, effective T cell-mediated immunity emanates from the expansion of specific T cells activated in response to antigen. In establishing and maintaining the peripheral T-cell pool, the thymus plays a critical role. It does so by providing a microenvironment within which T-cell precursors proliferate, differentiate and undergo selection processes to create a fully functional population of major histocompatibility complex restricted, self-tolerant T cells. The control of the thymic ...
Tópico(s): Circadian rhythm and melatonin
2008 - Elsevier BV | Autonomic Neuroscience
Vesna Pešić, Katarina Radojević, Duško Kosec, B. Plecas-Solarovic, Milica Perišić Nanut, Ivan Pilipović, Gordana Leposavić,
The role of gonadal hormones in induction and, particularly, maintenance/progression of rat thymic involution, which normally starts around puberty, was reassessed by examining the effects of peripubertal orchidectomy on thymic weight and morphometric parameters at different times up to the age of 10 months. Up to 6 months post-castration both thymic weight and cellularity in orchidectomized (Cx) rats were greater than in age-matched control rats, sham Cx (Sx). The increase in thymic cellularity reflected ...
Tópico(s): Hypothalamic control of reproductive hormones
2007 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Ivan Pilipović, Katarina Radojević, Milica Perišić Nanut, Duško Kosec, Mirjana Nacka-Aleksić, Jasmina Djikić, Gordana Leposavić,
Glucocorticoids have been shown to modulate the expression of noradrenaline metabolizing enzymes and β 2 ‐ and α 1B ‐adrenoceptors in a tissue‐ and cell‐ specific manner. In the thymus, apart from extensive sympathetic innervation, a regulatory network has been identified that encompasses catecholamine‐containing non‐lymphoid and lymphoid cells. We examined a putative role of adrenal‐ and thymus‐derived glucocorticoids in modulation of rat thymic noradrenaline levels and adrenoceptor expression. Seven ...
Tópico(s): Circadian rhythm and melatonin
2012 - Wiley | Experimental Physiology
Biljana Bufan, Stojic-Vukanic Zorica, Arsenovic-Ranin Nevena, Kosec Du�ko, Ivan Pilipović, Peri�ic Milica, �ikic Jasmina, Gordana Leposavić,
... Zorica Stojić-Vukanić Nevena Arsenović-Ranin Duško Kosec Ivan Pilipovic Milica Perišić Jasmina Đikić Gordana Leposavić Google Biljana Bufan Zorica Stojić-Vukanić Nevena Arsenović-Ranin Duško Kosec Ivan Pilipovic Milica Perišić Jasmina Đikić Gordana Leposavić Google Scholar Biljana Bufan Zorica Stojić-Vukanić Nevena Arsenović-Ranin Duško Kosec Ivan Pilipovic Milica Perišić Jasmina Đikić Gordana Leposavić PubMed Biljana Bufan Zorica Stojić-Vukanić Nevena Arsenović-Ranin Duško Kosec Ivan Pilipovic Milica Perišić Jasmina Đikić Gordana Leposavić Related Article in Frontiers ...
Tópico(s): Immune cells in cancer
2013 - Frontiers Media | Frontiers in Immunology
V. Stoičkov, Dragan Radovanović, Marina Deljanin-Ilić, Zoran Perišić, Milan Pavlović, I. Tasić, Ivan Stoičkov, Mlađjan Golubović, Aaron T. Scanlan, Vladimir Jakovljević, Emilija Stojanović,
Abstract The aim of this study was to compare QT dispersion (QTd) and echocardiographic parameters in male athletes competing across different sports (long-distance running, volleyball, football, powerlifting, and bodybuilding) and a control population. Significant moderate-strong differences (p < 0.001, $${\eta }_{p}^{2}$$ η p 2 = 0.52–0.71) were found in corrected QTd, intraventricular septal wall thickness (ISWT), posterior wall thickness (PWT), relative wall thickness (RWT) and LV (left ventricular) index between ...
Tópico(s): Cardiovascular Function and Risk Factors
2023 - Nature Portfolio | Scientific Reports
Ivan Pilipović, Katarina Radojević, Milica Perišić Nanut, Gordana Leposavić,
This paper highlights the multiple putative thymic and extrathymic points of intersection and interaction between glucocorticoids (GCs) and catecholamines (CAs)—the end‐point mediators of the major routes of communication between the brain and the immune system—in the context of intricate thymic T cell–developmental tuning. More specifically, we discuss in detail findings indicating that adrenal GCs can influence thymopoiesis by adjusting directly and/or indirectly (through modulation of pituitary ...
Tópico(s): T-cell and B-cell Immunology
2012 - Wiley | Annals of the New York Academy of Sciences
Ivan Pilipović, Katarina Radojević, Duško Kosec, Milica Perišić Nanut, Zorica Stojić-Vukanić, Nevena Arsenović-Ranin, Gordana Leposavić,
The study was undertaken considering that: i) androgens affect β2-adrenoceptor (AR)-mediated catecholamine (CA) action in many tissues; and ii) peripubertal changes in both circulating androgen and thymic CA levels are implicated in rat thymic involution. Its aims were to: i) explore putative effects of the late prepubertal orchidectomy on thymic CA:β2-AR complex in young adult rats, and ii) delineate the direct effects of testicular hormone withdrawal on the CA:β2-AR complex from those elicited secondarily ...
Tópico(s): Adrenal Hormones and Disorders
2013 - Elsevier BV | Journal of Neuroimmunology
Sylvia Nürnberg, Karen Cheng, Azad Raiesdana, Ramendra Kundu, Clint L. Miller, Juyong Brian Kim, Komal Arora, Iván Cárcamo-Orive, Yiqin Xiong, Nikhil Tellakula, Vivek Nanda, Nikitha Murthy, William A. Boisvert, Ulf Hedin, Ljubica Perisic, Silvia Aldi, Lars Mäegdefessel, Milos Pjanic, Gary K. Owens, Michelle D. Tallquist, Thomas Quertermous,
Recent genome wide association studies have identified a number of genes that contribute to the risk for coronary heart disease. One such gene, TCF21, encodes a basic-helix-loop-helix transcription factor believed to serve a critical role in the development of epicardial progenitor cells that give rise to coronary artery smooth muscle cells (SMC) and cardiac fibroblasts. Using reporter gene and immunolocalization studies with mouse and human tissues we have found that vascular TCF21 expression in ...
Tópico(s): Williams Syndrome Research
2015 - Public Library of Science | PLoS Genetics
Sylvia Nürnberg, Kailin Cheng, Azad Raiesdana, Ramendra Kundu, Clint L. Miller, Juyong Brian Kim, Kanika Arora, Iván Cárcamo-Orive, Yuning Xiong, Nikhil Tellakula, Vivek Nanda, Nikitha Murthy, William A. Boisvert, Ulf Hedin, Ljubica Perisic, Silvia Aldi, Lars Mäegdefessel, Milos Pjanic, Gary K. Owens, Michelle D. Tallquist, Thomas Quertermous,
TCF21 is a basic helix–loop–helix transcription factor that has recently been implicated as contributing to susceptibility to coronary heart disease based on genome wide association studies. In order to identify transcriptionally regulated target genes in a major disease relevant cell type, we performed siRNA knockdown of TCF21 in in vitro cultured human coronary artery smooth muscle cells and compared the transcriptome of siTCF21 versus siCONTROL treated cells. The raw (FASTQ) as well as processed ( ...
Tópico(s): Cancer-related molecular mechanisms research
2015 - Elsevier BV | Genomics Data
Milica Perišić Nanut, Duško Kosec, Ivan Pilipović, Radojevic Katarina, Pesic Vesna, Rakin Ana, Gordana Leposavić,
The present study was undertaken to reassess the recently challenged role of ovarian hormones in age-associated thymic involution. For this purpose, in eleven-month-old peripubertally ovariectomized (Ox) rats we analyzed: i) thymic weight and cellularity, ii) size of CD4+CD8+ double-positive (DP) thymocyte population, which is believed to correlate to the thymic capacity to export mature T cells, iii) number of recent thymic emigrants (RTEs), and iv) number of peripheral blood CD4+ and CD8+ lymphocytes. ...
Tópico(s): T-cell and B-cell Immunology
2009 - De Gruyter Open | Acta veterinaria