Produção Nacional
Revisado por pares
Júlia Buonafina da Silva, Helena Vitória Silva Pinheiro, Júlia Larissa de Souza Silva, Maurizene Rodrigues Silva, Marília Juliane Pedrosa Gurgel, Gabriela Portela, Rosário Antunes Fonseca Lima,
Tópico(s): Healthcare during COVID-19 Pandemic
2021 - | Revista Enfermagem Digital Cuidado e Promoção da Saúde
Produção Nacional
Revisado por pares
Jonas Botelho, Stefany Pinheiro, Júlia G. Ribeiro, Carlos Souza,
This study presents our efforts to automate the detection of unofficial roads (herein, roads) in the Brazilian Amazon using artificial intelligence (AI). In this region, roads are built by loggers, goldminers, and unauthorized land settlements from existing official roads, expanding over pristine forests and leading to new deforestation and fire hotspots. Previous research used visual interpretation, hand digitization, and vector editing techniques to create a thorough Amazon Road Dataset (ARD) from ...
Tópico(s): Wildlife-Road Interactions and Conservation
2022 - Multidisciplinary Digital Publishing Institute | Remote Sensing
Michael von Wolff, S. Rösner, C. Thöne, Rafael Mota Pinheiro, Julia Jauckus, Thomas Brückner, Vanderleyen Biolchi, Amal Alia, T. Strowitzki,
ObjectiveTo evaluate whether intravaginal application of seminal plasma at the time of follicle aspiration in IVF or intracytoplasmic sperm injection treatment cycles has the potential to increase pregnancy rate. To calculate the number of patients needed to achieve significance in a multicenter trial.DesignDouble-blind, placebo-controlled randomized pilot study.SettingUniversity department of gynecological endocrinology and reproductive medicine.Patient(s)One hundred sixty-eight patients undergoing ...
Tópico(s): Urologic and reproductive health conditions
2008 - Elsevier BV | Fertility and Sterility
Diogo L. Pinheiro, Julia Melkers, Jan Youtie,
The need to encourage future generations of students in the pursuit of scientific research has been viewed as a cornerstone of US R&D efforts. However, few indicators of student intellectual activity at the graduate level are tracked on an ongoing basis. The aim of this paper is to examine graduate student publishing as an indicator of pre-doctoral research activity and future scholarly success. This study addresses the gap in knowledge about student publishing through a distinctive dataset that ...
Tópico(s): Higher Education Research Studies
2012 - Elsevier BV | Technological Forecasting and Social Change
Carla Pinheiro, Julia Kehr, Cândido Pinto Ricardo,
Tópico(s): Plant Reproductive Biology
2005 - Springer Science+Business Media | Planta
Produção Nacional
Revisado por pares
Kalil Alves de Lima, Gislane Lelis Vilela de Oliveira, Juliana Navarro Ueda Yaochite, Daniel Guariz Pinheiro, Júlia Teixeira Cottas de Azevedo, Wilson A. Silva, Dimas Tadeu Covas, Carlos Eduardo Barra Couri, Belinda Pinto Simões, Júlio César Voltarelli, Maria Carolina Oliveira, Kelen Cristina Ribeiro Malmegrim,
Bone marrow multipotent mesenchymal stromal cells (MSCs) are a diverse subset of precursors that contribute to the homeostasis of the hematopoietic niche. MSCs can be isolated and expanded in vitro and have unique immunomodulatory and regenerative properties that make them attractive for the treatment of autoimmune diseases, including type 1 diabetes (T1D). Whether autologous or allogeneic MSCs are more suitable for therapeutic purposes has not yet been established. While autologous MSCs may present ...
Tópico(s): Hematopoietic Stem Cell Transplantation
2016 - BioMed Central | Stem Cell Research & Therapy
Produção Nacional
Revisado por pares
Ricardo Alamino Figueiredo, C. M. Barros, Osni L. Pinheiro, Júlia Maria Pavan Soler,
The most common beef cattle raised in Brazil is the Nelore breed (Bos indicus). Information obtained by ultrasonography on follicular growth in Bos taurus cattle has been accumulating rapidly. However, there are few publications to date on follicular development in Bos indicus breeds. The follicular dynamics in Nelore heifers and cows during natural or prostaglandin (PG)-induced estrous cycle were studied. From the detection of estrus onward, all animals were examined daily by ultrasonography for ...
Tópico(s): Effects of Environmental Stressors on Livestock
1997 - Elsevier BV | Theriogenology
Diogo L. Pinheiro, Julia Melkers, Sunni Newton,
Placement in prestigious research institutions for STEM (science, technology, engineering, and mathematics) PhD recipients is generally considered to be optimal. Yet some doctoral recipients are not interested in intensive research careers and instead seek alternative careers, outside but also within academe (for example teaching positions in Liberal Arts Schools). Recent attention to non-academic pathways has expanded our understanding of alternative PhD careers. However, career preferences and placements ...
Tópico(s): Higher Education Governance and Development
2017 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Lucas Siqueira Pinheiro, Júlia Eick Iglesias, Daiana Boijink, Letícia Boldrin Mestieri, Patrícia Maria Poli Kopper, José Antônio de Poli Figueiredo, Fabiana Soares Grecca,
The aim of this study was to evaluate the cell viability and tissue reaction of NeoMTA Plus (NMP; Avalon Biomed Inc, Houston, TX) compared with mineral trioxide aggregate (MTA; Angelus, Londrina, PR, Brazil) and Biodentine (BD; Septodont, Saint-Maur-de-Fossés, France).Fibroblasts (3T3) were plated and exposed to 1% extract from the test material before and after setting. Cytotoxicity assessment was performed using the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide and sulforhodamine B assays. ...
Tópico(s): Dental Implant Techniques and Outcomes
2018 - Elsevier BV | Journal of Endodontics
Produção Nacional
Revisado por pares
Laís Kohan, Cristiane Reis Martins, Larissa Oliveira Duarte, Luciano Pinheiro, Júlia Baruque-Ramos,
Footwear material is of great importance mainly considering the user’s health. There are many components in contact with upper and mainly lower part of foot, being the sole essential part to weight distribution, muscle activity and vertebral column. This study aimed to verify how the natural fibers are applied at Brazilian market footwear, demonstrating the types of raw materials utilized, textile constructions and other variables. As well as comparing the information at natural fibers applications ...
Tópico(s): Physical Education and Gymnastics
2019 - Springer Nature | SN Applied Sciences
Maísa Pinheiro, Julia C. Gage, Gary M. Clifford, Maria Demarco, Li C. Cheung, Zigui Chen, Meredith Yeager, Michael Cullen, Joseph F. Boland, Xiaojian Chen, Tina Raine‐Bennett, Mia Steinberg, Sara Bass, Brian Befano, Yanzi Xiao, Vanessa Tenet, Joan L. Walker, Rosemary E. Zuna, Nancy Poitras, Michael A. Gold, T.S. Dunn, Kai Yu, Bin Zhu, Laurie Burdett, Sevilay Turan, Thomas Lorey, Philip E. Castle, Nicolas Wentzensen, Robert D. Burk, Mark Schiffman, Lisa Mirabello,
HPV35 has been found in only ∼2% of invasive cervical cancers (ICC) worldwide but up to 10% in Sub-Saharan Africa, warranting further investigation and consideration of impact on preventive strategies. We studied HPV35 and ethnicity, in relation to the known steps in cervical carcinogenesis, using multiple large epidemiologic studies in the U.S. and internationally. Combining five U.S. studies, we measured HPV35 positivity and, in Northern California, observed HPV35 type-specific population prevalence ...
Tópico(s): Reproductive tract infections research
2020 - Wiley | International Journal of Cancer
Produção Nacional
Revisado por pares
Gabriela Cardoso Ferreira, Lucas Siqueira Pinheiro, Júlia Silveira Nunes, Roberta de Almeida Mendes, Cláudia Daniela Schuster, Renata Grazziotin Soares, Patrícia Maria Poli Kopper, José Antônio Poli de Figueiredo, Fabiana Soares Grecca,
Abstract Biocompatibility, dimensional stability, radiopacity, flow, and low solubility are the characteristics of an ideal endodontic sealer. This study evaluated and compared in vivo and in vitro biological and physicochemical properties of calcium silicate‐based sealers: Sealer Plus BC (BC), MTA Fillapex (MF); and resin‐based sealers: AH Plus (AHP) and Sealer Plus (SP). Apical papilla cells were exposed to sealer extracts and subjected to MTT, SRB, scratch, alkaline phosphatase enzyme activity (ALP) ...
Tópico(s): Dental Radiography and Imaging
2021 - Wiley | Journal of Biomedical Materials Research Part B Applied Biomaterials
Produção Nacional
Revisado por pares
Júlia Pinheiro Chagas da Cunha, Pedro A. F. Galante, Jorge Estefano de Souza, Robson Francisco de Souza, Patrícia M. Carvalho, Daniel T. O’Hara, Ricardo P. Moura, S. M. Oba-Shinja, Suely Kazue Nagahashi Marie, Wilson A. Silva, Rodrigo Oliva Perez, Beatriz Stransky, Martin Pieprzyk, Jason H. Moore, O L Caballero, J. Gama‐Rodrigues, Angelita Habr‐Gama, Winston Patrick Kuo, Andrew Simpson, Anamaria A. Camargo, Lloyd J. Old, Sandro J. de Souza,
Cell surface proteins are excellent targets for diagnostic and therapeutic interventions. By using bioinformatics tools, we generated a catalog of 3,702 transmembrane proteins located at the surface of human cells (human cell surfaceome). We explored the genetic diversity of the human cell surfaceome at different levels, including the distribution of polymorphisms, conservation among eukaryotic species, and patterns of gene expression. By integrating expression information from a variety of sources, ...
Tópico(s): Advanced Proteomics Techniques and Applications
2009 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Produção Nacional
Revisado por pares
Sheila Cristina Nardelli, Júlia Pinheiro Chagas da Cunha, María Cristina M. Motta, Sérgio Schenkman,
Tópico(s): HIV/AIDS drug development and treatment
2009 - Springer Science+Business Media | Chromosoma
Produção Nacional
Luciana M. Gutiyama, Júlia Pinheiro Chagas da Cunha, Sérgio Schenkman,
ABSTRACT Phosphorylation of histone H1 is intimately related to the cell cycle progression in higher eukaryotes, reaching maximum levels during mitosis. We have previously shown that in the flagellated protozoan Trypanosoma cruzi , which does not condense chromatin during mitosis, histone H1 is phosphorylated at a single cyclin-dependent kinase site. By using an antibody that recognizes specifically the phosphorylated T. cruzi histone H1 site, we have now confirmed that T. cruzi histone H1 is also ...
Tópico(s): Signaling Pathways in Disease
2008 - American Society for Microbiology | Eukaryotic Cell
Produção Nacional
Revisado por pares
Eliana Moreira Pinheiro, Maria Júlia Paes da Silva, Margareth Ângelo, Circéa Amália Ribeiro,
This study aimed to understand the meaning of the nursing team's communication with newborns and families during care delivered in a neonatal unit and also to develop a theoretical model. Symbolic Interactionism and Hargie's model were used as theoretical and Grounded theory as the methodological reference framework. The study was carried out at the nursery of a hospital in São Paulo, SP, Brazil. The study sample was composed of three nurses and four nursing auxiliaries. The theoretical model Being ...
Tópico(s): Breastfeeding Practices and Influences
2008 - UNIVERSIDADE DE SÃO PAULO | Revista Latino-Americana de Enfermagem
Produção Nacional
Revisado por pares
Maria Carolina Elias, Júlia Pinheiro Chagas da Cunha, F DEFARIA, Renato A. Mortara, Edna Freymüller, Sérgio Schenkman,
The replication and segregation of organelles producing two identical daughter cells must be precisely controlled during the cell cycle progression of eukaryotes. In kinetoplastid flagellated protozoa, this includes the duplication of the single mitochondrion containing a network of DNA, known as the kinetoplast, and a flagellum that grows from a cytoplasmic basal body through the flagellar pocket compartment before emerging from the cell. Here, we show the morphological events and the timing of ...
Tópico(s): Biochemical and Molecular Research
2006 - Elsevier BV | Protist
Produção Nacional
Revisado por pares
Júlia Pinheiro Chagas da Cunha, Ernesto Nakayasu, Igor C. Almeida, Sérgio Schenkman,
Histone tails provide sites for a variety of post-translational modifications implicated in the control of gene expression and chromatin assembly. As both histones and control of gene expression in trypanosomes are highly divergent compared to most eukaryotes, post-translational modifications of Trypanosoma cruzi histones were investigated. After in vivo incubation of live parasites with radiolabeled precursors, histone H4 mainly incorporates [3H]-acetyl, and to a lesser extent [3H]-methyl residues. ...
Tópico(s): Biochemical and Molecular Research
2006 - Elsevier BV | Molecular and Biochemical Parasitology
Produção Nacional
Revisado por pares
Júlia Pinheiro Chagas da Cunha, Ernesto Nakayasu, Maria Carolina Elias, Daniel C. Pimenta, Marı́a Teresa Téllez-Iñón, Federico Rojas, Manuel J. Muñoz, Igor C. Almeida, Sérgio Schenkman,
Histone H1 of most eukaryotes is phosphorylated during the cell cycle progression and seems to play a role in the regulation of chromatin structure, affecting replication and chromosome condensation. In trypanosomatids, histone H1 lacks the globular domain and is shorter when compared with the histone of other eukaryotes. We have previously shown that in Trypanosoma cruzi, the agent of Chagas' disease, histone H1 is phosphorylated and this increases its dissociation from chromatin. Here, we demonstrate ...
Tópico(s): Calcium signaling and nucleotide metabolism
2005 - Elsevier BV | Molecular and Biochemical Parasitology
Produção Nacional
Revisado por pares
Larissa V. G. Longo, Júlia Pinheiro Chagas da Cunha, Tiago J. P. Sobreira, Rosana Puccia,
We identified non-covalently linked cell wall proteins from Paracoccidioides brasiliensis yeasts and mycelia, with focus on the yeast pathogenic phase, and correlated them with reported fungal extracellular vesicle proteins. We studied isolates Pb3 and Pb18, which evoke distinct patterns of experimental paracoccidioidomycosis and represent two phylogenetic groups. Proteins were extracted mildly with dithiothreitol, trypsinized, and peptides analyzed by liquid chromatography coupled to high-resolution ...
Tópico(s): Yeasts and Rust Fungi Studies
2014 - Elsevier BV | EuPA Open Proteomics
Produção Nacional
Revisado por pares
Teresa Cristina Leandro de Jesus, Vinícius Santana Nunes, Mariana Lopes, Daiana Evelin Martil, Leo Kei Iwai, Nilmar Silvio Moretti, Fabrício C. Machado, Mariana Leão de Lima-Stein, Otávio Henrique Thiemann, Maria Carolina Elias, Christian J. Janzen, Sérgio Schenkman, Júlia Pinheiro Chagas da Cunha,
Histones are well-conserved proteins that form the basic structure of chromatin in eukaryotes and undergo several post-translational modifications, which are important for the control of transcription, replication, DNA damage repair, and chromosome condensation. In early branched organisms, histones are less conserved and appear to contain alternative sites for modifications, which could reveal evolutionary unique functions of histone modifications in gene expression and other chromatin-based processes. ...
Tópico(s): Biochemical and Molecular Research
2016 - American Chemical Society | Journal of Proteome Research
Produção Nacional
Revisado por pares
Teresa Cristina Leandro de Jesus, Simone Guedes Calderano, Francisca Nathália de Luna Vitorino, Ricardo Pariona-Llanos, Mariana Lopes, Christiane B. de Araujo, Otávio Henrique Thiemann, Marcelo S. Reis, Maria Carolina Elias, Júlia Pinheiro Chagas da Cunha,
Chromatin associated proteins are key regulators of many important processes in the cell. Trypanosoma cruzi, a protozoa flagellate that causes Chagas disease, alternates between replicative and nonreplicative forms accompanied by a shift on global transcription levels and by changes in its chromatin architecture. Here, we investigated the T. cruzi chromatin proteome using three different protocols and compared it between replicative (epimastigote) and nonreplicative (trypomastigote) forms by high- ...
Tópico(s): Biochemical and Molecular Research
2016 - Elsevier BV | Molecular & Cellular Proteomics
Produção Nacional
Revisado por pares
Marcelo Augusto Kazuo Ikeda, José Roberto Fogaça de Almeida, Grasielle Pereira Jannuzzi, André Cronemberger Andrade, Ana Cláudia Torrecilhas, Nilmar Silvio Moretti, Júlia Pinheiro Chagas da Cunha, Sandro Rogério de Almeida, Karen Spadari Ferreira,
Sporotrichosis is a mycosis that affects the skin, lymphatic system and other organs in humans and animals. The disease has a worldwide distribution, with endemic areas in Brazil, and is caused by a complex of species, including Sporothrix brasiliensis. Some fungi release extracellular vesicles (EVs) that can interact with the host cell and modulate the host immune response. The aim of this study was to analyze the participation of S. brasiliensis EVs in the modulation of dendritic cells (DCs) and ...
Tópico(s): Galectins and Cancer Biology
2018 - Frontiers Media | Frontiers in Microbiology
Produção Nacional
Revisado por pares
Guilherme Henrique Gatti da Silva, Melissa S. Jurica, Júlia Pinheiro Chagas da Cunha, Carla C. Oliveira, Patrícia P. Coltri,
Pre-messenger RNA (mRNA) splicing is an essential step in the control of eukaryotic gene expression. During splicing, the introns are removed from the gene transcripts as the exons are ligated to create mature mRNA sequences. Splicing is performed by the spliceosome, which is a macromolecular complex composed of five small nuclear RNAs (snRNAs) and more than 100 proteins. Except for the core snRNP proteins, most spliceosome proteins are transiently associated and presumably involved with the regulation ...
Tópico(s): RNA and protein synthesis mechanisms
2018 - Wiley | Journal of Cellular Biochemistry
Produção Nacional
Revisado por pares
Simone Sidoli, Yekaterina Kori, Mariana Lopes, Zuo‐Fei Yuan, Hee Jong Kim, Katarzyna Kulej, Kevin A. Janssen, Laura M. Agosto, Júlia Pinheiro Chagas da Cunha, Andrew J. Andrews, Benjamin A. García,
DNA and histone proteins define the structure and composition of chromatin. Histone posttranslational modifications (PTMs) are covalent chemical groups capable of modeling chromatin accessibility, mostly due to their ability in recruiting enzymes responsible for DNA readout and remodeling. Mass spectrometry (MS)-based proteomics is the methodology of choice for large-scale identification and quantification of protein PTMs, including histones. High sensitivity proteomics requires online MS coupling ...
Tópico(s): RNA modifications and cancer
2019 - Cold Spring Harbor Laboratory Press | Genome Research
Produção Nacional
Revisado por pares
Roberta Peres da Silva, Larissa G. V. Longo, Júlia Pinheiro Chagas da Cunha, Tiago J. P. Sobreira, Márcio L. Rodrigues, Helisson Faoro, Samuel Goldenberg, Lysangela R. Alves, Rosana Puccia,
Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, ...
Tópico(s): Galectins and Cancer Biology
2019 - Multidisciplinary Digital Publishing Institute | Cells
Produção Nacional
Revisado por pares
Christiane B. de Araujo, Júlia Pinheiro Chagas da Cunha, Davi Toshio Inada, Jeziel D. Damasceno, Alex Ranieri Jerônimo Lima, Priscila M. Hiraiwa, Catarina A. Marques, Evonnildo Costa Gonçalves, Milton Yutaka Nishiyama, Richard McCulloch, Maria Carolina Elias,
Abstract Background DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi , the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown. Results Here we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only ...
Tópico(s): CRISPR and Genetic Engineering
2020 - BioMed Central | BMC Genomics
Produção Nacional
Revisado por pares
Hátylas Azevedo, Guilherme Cavalcante Pessoa, Francisca Nathália de Luna Vitorino, Jérémie Nsengimana, Julia Newton‐Bishop, Eduardo M. Reis, Júlia Pinheiro Chagas da Cunha, Miriam Galvonas Jasiulionis,
Abstract Background We have previously developed a murine cellular system that models the transformation from melanocytes to metastatic melanoma cells. This model was established by cycles of anchorage impediment of melanocytes and consists of four cell lines: differentiated melanocytes (melan-a), pre-malignant melanocytes (4C), malignant (4C11−), and metastasis-prone (4C11+) melanoma cells. Here, we searched for transcriptional and epigenetic signatures associated with melanoma progression and metastasis ...
Tópico(s): Cancer Cells and Metastasis
2020 - BioMed Central | Clinical Epigenetics
Produção Nacional
Revisado por pares
Loyze Paola de Lima, Saloê Bispo Poubel, Zuo‐Fei Yuan, Juliana Nunes Rosón, Francisca Nathália de Luna Vitorino, Fabíola Holetz, Benjamin A. García, Júlia Pinheiro Chagas da Cunha,
Tópico(s): Genetics and Neurodevelopmental Disorders
2020 - Elsevier BV | Journal of Proteomics
Christine Loignon, Sophie Dupére, Caroline Leblanc, Karoline Truchon, Amélie Bouchard, Johanne Arsenault, Julia Pinheiro Carvalho, Alexandrine Boudreault‐Fournier, Sylvain Aimé Marcotte,
Abstract To improve health equity, as well as equity in research, community-engaged research and participatory research needs to be inclusive. Equity in health research refers to the principle that anyone affected by research or who can benefit from its outcomes should have equal opportunities to contribute to it. Many researchers advocate the importance of promoting equity in research and engage in processes that foster the research involvement of lay persons, patients, and community members who ...
Tópico(s): Health Policy Implementation Science
2021 - BioMed Central | Research Involvement and Engagement