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... Social: Court Circular. Obituaries: Baron Erik Palmstierna, Professor Jan Wiegers, Rev. Charles Marcon, Mr. A. Lawson-Walton. Law: ...
1959 - Gale Group | TDA
G. Jan Wiegers, Johannes M. H. M. Reul,
... not sufficient and needs to be extended. Here, Jan Wiegers and Hans Reul ask how these apparently paradoxical ...
Tópico(s): T-cell and B-cell Immunology
1998 - Elsevier BV | Trends in Pharmacological Sciences
Kor Venema, Wieger Leever, Jan O. Bakker, Ger Haayer, Jakob Korf,
A device to derivatize amino acids with o-phthaldialdehyde, which is directly connected to high-performance liquid chromatographic equipment is described. Its principle is that a sample (10-500 microliters) is mixed with a reagent (containing o-phthaldialdehyde, 2-mercaptoethanol and sodium hydrogen carbonate buffer), using a peristaltic pump. This mixture is pumped into a loop of a pneumatically controlled injection valve at atmospheric pressure. When the derivatization is complete the valve switches, ...
Tópico(s): Microfluidic and Capillary Electrophoresis Applications
1983 - Elsevier BV | Journal of Chromatography A
Muhammad Mansha, Michela Carlet, Christian Ploner, Georg B. Gruber, Muhammad Wasim, Gerrit Jan Wiegers, Johannes Rainer, Stephan Geley, Reinhard Kofler,
Glucocorticoids (GCs) cause apoptosis and cell cycle arrest in lymphoid cells and are used in the therapy of lymphoid malignancies. SLA (Src-like-adaptor), an inhibitor of T- and B-cell receptor signaling, is a promising candidate derived from expression profiling analyses in children with acute lymphoblastic leukemia (ALL). Over-expression and knock-down experiments in ALL in vitro model revealed that transgenic SLA alone had no effect on survival or cell cycle progression, nor did it affect sensitivity ...
Tópico(s): Neuroblastoma Research and Treatments
2009 - Elsevier BV | Leukemia Research
Johannes M. H. M. Reul, Ingemar S.M. Stec, G. Jan Wiegers, Marta Labeur, Astrid C. E. Linthorst, Eduardo Arzt, Herta Flor,
We investigated whether non-abortive maternal infections would compromise fetal brain development and alter hypothalamic-pituitary-adrenocortical (HPA) axis functioning when adult. To study putative teratogenic effects of a T cell-mediated immune response versus an endotoxic challenge, 10-d-pregnant rats received a single intraperitoneal injection of 5 x 10(8) human red blood cells (HRBC) or gram-negative bacterial endotoxin (Escherichia coli LPS: 30 micrograms/kg). The adult male progeny (3 mo old) ...
Tópico(s): Birth, Development, and Health
1994 - American Society for Clinical Investigation | Journal of Clinical Investigation
G. Jan Wiegers, Johannes M. H. M. Reul, Herta Flor, E. R. de Kloet,
Numerous previous studies investigating the effects of corticosteroids on immune proliferation reported almost unanimously inhibitory effects. However, in many of these studies, high concentrations of corticosteroids (micromolar range) and long incubation times (days) were used. We have investigated whether corticosteroid hormones at low physiological (nanomolar) vs. high (micromolar) concentrations have distinct effects on immune cells after short term (minutes) as opposed to long term preexposure (hours). ...
Tópico(s): Adrenal Hormones and Disorders
1994 - Oxford University Press | Endocrinology
Journal Article Enhancement of rat splenic lymphocyte mitogenesis after short term preexposure to corticosteroids in vitro Get access G J Wiegers, G J Wiegers 1Max-Planck Institute of Psychiatry, Department of Neuroendocrinology, Munich, Germany. Search for other works by this author on: Oxford Academic Google Scholar J M Reul, J M Reul 1Max-Planck Institute of Psychiatry, Department of Neuroendocrinology, Munich, Germany. Search for other works by this author on: Oxford Academic Google Scholar ...
Tópico(s): Hormonal Regulation and Hypertension
1994 - Oxford University Press | Endocrinology
G. Jan Wiegers, Manuel Kaufmann, Denise Tischner, Andreas Villunger,
Thymocyte selection aims to shape a T‐cell repertoire that, on the one hand, is able to recognize and respond to foreign peptides and, on the other hand, tolerizes the presence of self‐peptides in the periphery. Deletion of T cells or their precursors that fail to fulfill these criteria is mainly mediated by the Bcl‐2‐regulated apoptosis pathway. Absence of T‐cell receptor (TCR)‐mediated signals or hyperactivation of the TCR by high‐affinity self‐peptide‐major histocompatibility complexes can both ...
Tópico(s): Immunotherapy and Immune Responses
2010 - Wiley | Immunology and Cell Biology
... case studies were enabled by Citogroup. Participating schools, Jan Wiegers, Peter Hermans, Julet Harms and Kirsten van Ingen ...
Tópico(s): Business Strategy and Innovation
2007 - Taylor & Francis | Journal of Education Policy
Denise Tischner, G. Jan Wiegers, Heidi Fiegl, Mathias Drach, Andreas Villunger,
Transforming growth factor beta (TGF-β)- and Interleukin-2 (IL-2)-mediated signaling enables the generation and expansion of induced regulatory T (iTreg) cells that carry high hopes for the treatment of chronic inflammatory and autoimmune diseases. Knowledge about factors stabilizing their lineage commitment and lifespan, however, is limited. Here, we investigated the behavior of iTreg cells, derived from apoptosis-defective mouse mutants, during activated cell autonomous cell death, triggered by cytokine- ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2012 - Springer Nature | Cell Death and Differentiation
Stefan M. Brunner, Dietmar Herndler‐Brandstetter, C. Arnold, Gerrit Jan Wiegers, Andreas Villunger, Matthias Hackl, Johannes Grillari, María Moreno‐Villanueva, Alexander Bürkle, Beatrix Grubeck‐Loebenstein,
The life-long homeostasis of memory CD8(+) T cells as well as persistent viral infections have been shown to facilitate the accumulation of highly differentiated CD8(+) CD28(-) T cells, a phenomenon that has been associated with an impaired immune function in humans. However, the molecular mechanisms regulating homeostasis of CD8(+) CD28(-) T cells have not yet been elucidated. In this study, we demonstrate that the miR-23∼24∼27 cluster is up-regulated during post-thymic CD8(+) T-cell differentiation ...
Tópico(s): Cancer therapeutics and mechanisms
2012 - Wiley | Aging Cell
Denise Tischner, Irene Gaggl, Ines Peschel, Manuel Kaufmann, Selma Tuzlak, Mathias Drach, Niκolaus Thuille, Andreas Villunger, G. Jan Wiegers,
The Bcl-2 regulated apoptosis pathway is critical for the elimination of autoreactive lymphocytes, thereby precluding autoimmunity. T cells escaping this process can be kept in check by regulatory T (Treg) cells expressing the transcription and lineage commitment factor Foxp3. Despite the well-established role of Bcl-2 family proteins in shaping the immune system and their frequent deregulation in autoimmune pathologies, it is poorly understood how these proteins affect Treg cell development and ...
Tópico(s): Immunotherapy and Immune Responses
2012 - Elsevier BV | Journal of Autoimmunity
G. Jan Wiegers, Gerda Croiset, Johannes M. H. M. Reul, Herta Flor, E. R. de Kloet,
The effects of corticosteroids were studied on the concanavalin A (Con A)-induced mitogenesis of peripheral blood T-lymphocytes obtained from intact and adrenalectomized (ADX) Wistar rats. One week of adrenalectomy reduced the proliferative response of T-cells by 65% compared with sham-operated controls. Substitution of ADX rats with subcutaneously implanted 12.5-mg corticosterone (Cort) pellets, which resulted in low circulating Cort levels (17 +/- 3 nM), restored the reduced proliferative capacity to ...
Tópico(s): Stress Responses and Cortisol
1993 - American Physiological Society | AJP Endocrinology and Metabolism
G. Jan Wiegers, Ilona E. M. Stec, Philipp Sterzer, Johannes M. H. M. Reul,
Despite the importance of glucocorticoids (GCs) to modern medicine, the physiological role of endogenous GCs in immunomodulation is poorly understood. Evidence collected over the past decade convincingly shows that GCs at levels that can be reached physiologically affect the immune response in a more differentiated way than previously thought. This chapter discusses evidence suggesting that endogenous GCs not only suppress but also direct and enhance immune functions. Antigen-specific immunity, for ...
Tópico(s): Immune Response and Inflammation
2005 - Elsevier BV | Techniques in the behavioral and neural sciences
Miriam Erlacher, Michael Knoflach, Ilona E. M. Stec, Günther Böck, Georg Wick, G. Jan Wiegers,
Signaling by either the TCR or glucocorticoid receptor (GR) induces apoptosis in thymocytes. Interestingly, it has been shown previously that hybridoma T cells escape apoptosis induced by either TCR or GR when both of these receptors signal simultaneously. Whether such mutual antagonism is present in primary thymocytes was the subject of the present study. Both glucocorticoids (GC) and anti-TCR/CD28 (or anti-CD3/CD28) mAb induced apoptosis in total thymocytes. When these signals were present at the ...
Tópico(s): Immunotherapy and Immune Responses
2005 - Wiley | European Journal of Immunology
Oskar Lechner, G. Jan Wiegers, Antonio J. Oliveira-dos-Santos, Hermann Dietrich, Heidrun Recheis, Michael R. Waterman, Richard L. Boyd, Georg Wick,
Glucocorticoid hormones are known to act as important modulatory factors in the development of autoimmune diseases, and to play an important role in thymic T-cell selection. There seems to be a finely balanced equilibrium between the apoptosis-inducing effects of glucocorticoid and T cell receptor ligand binding. Here we are investigating whether glucocorticoid-induced T cell apoptosis is mainly dependent on circulating glucocorticoid levels or if the thymus itself is able to produce glucocorticoids. ...
Tópico(s): T-cell and B-cell Immunology
2000 - Wiley | European Journal of Immunology
G. Jan Wiegers, Ilona E. M. Stec, Wolfgang E. F. Klinkert, Johannes M. H. M. Reul,
CD4 serves as a coreceptor during Ag recognition by the TCR. This interaction results in a marked increase in the sensitivity of a T cell to Ag presented by MHC class II molecules. Here we report that activation of T cells either by plate-bound mAb (anti-TCR, anti-CD3) or soluble activators (staphylococcal enterotoxin A, Con A) is associated with an (up to 3-fold) increase in CD4 cell surface expression on CD25+ cells, which was maximal after 72-96 h. Incubation with the glucocorticoid hormone corticosterone ( ...
Tópico(s): HIV Research and Treatment
2000 - American Association of Immunologists | The Journal of Immunology
Oskar Lechner, H. Dietrich, Andreza O. Santos, G. Jan Wiegers, S. Schwarz, M Harbutz, Manfred Herold, Georg Wick,
Tópico(s): Adrenal Hormones and Disorders
2000 - Elsevier BV | Journal of Autoimmunity
S.C. Steenman, Wieger Bakker, Jan van Tartwijk,
The first-year grade point average (FYGPA) is the predominant measure of student success in most studies on university admission. Previous cognitive achievements measured with high school grades or standardized tests have been found to be the strongest predictors of FYGPA. For this reason, standardized tests measuring cognitive achievement are widely used as a tool for selective admission to higher education. The FYGPA, however, measures many markedly different aspects of student success. In this ...
Tópico(s): Student Assessment and Feedback
2014 - Routledge | Studies in Higher Education
Lára Hannesdóttir, Piotr Tymoszuk, Nirmala Parajuli, Marie-Hélène Wasmer, Sonja Philipp, Nina Daschil, Sebak Datta, Johann‐Benedikt Koller, Christoph H. Tripp, Patrizia Stoitzner, Elisabeth Müller‐Holzner, Gerrit Jan Wiegers, Veronika Sexl, Andreas Villunger, Wolfgang Doppler,
The dual erbB1/2 tyrosine kinase inhibitor lapatinib as well as the anthracycline doxorubicin are both used in the therapy of HER2-positive breast cancer. Using MMTV-neu mice as an animal model for HER2-positive breast cancer, we observed enhanced tumor infiltration by IFN-γ-secreting T cells after treatment with doxorubicin and/or lapatinib. Antibody depletion experiments revealed a contribution of CD8⁺ but not CD4⁺ T cells to the antitumor effect of these drugs. Doxorubicin treatment additionally ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2013 - Wiley | European Journal of Immunology
Marta Labeur, Eduardo Arzt, G. Jan Wiegers, Herta Flor, Johannes M. H. M. Reul,
The effects of long-term corticotropin-releasing hormone (CRH) infusion in the lateral ventricle of the rat on hypothalamic-pituitary-adrenocortical (HPA) axis parameters and on the immune system function were studied. Compared with infusion of vehicle, the CRH treatment produced a sustained overactivity of the HPA axis, as evidenced by elevated plasma ACTH and corticosterone levels, increased anterior pituitary POMC messenger RNA (mRNA) expression, and adrenal enlargement. Long-term CRH treatment ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
1995 - Oxford University Press | Endocrinology
G. Jan Wiegers, Marta Labeur, Ilona E. M. Stec, Wolfgang E. F. Klinkert, Herta Flor, Johannes M. H. M. Reul,
To study steroid regulation of cell-mediated immunity, we used anti-TCR-stimulated rat splenic lymphocyte mitogenesis as our experimental paradigm. Surprisingly, we found that the principal glucocorticoid of the rat, corticosterone (CORT), potently enhanced anti-TCR-induced lymphocyte proliferation after 2 to 3 days in culture, followed by inhibited cell growth after 5 to 7 days. Thus, glucocorticoids appeared to accelerate anti-TCR-induced lymphocyte mitogenesis. This effect occurred at physiologic ...
Tópico(s): Hormonal Regulation and Hypertension
1995 - American Association of Immunologists | The Journal of Immunology
Oskar Lechner, Hermann Dietrich, G. Jan Wiegers, Melanie S. Vacchio, Georg Wick,
Glucocorticoid (GC) hormones play an important role in thymic T cell selection and in the development of autoimmune diseases. Previous studies have shown that the mammalian thymus itself is able to produce GC. In order to assess the importance of these findings in terms of the evolutionary development of the immune system, we investigated the functional presence of steroidogenic enzymes in primary lymphoid organs of chickens, which represent one of the best studied non-mammalian species. To this ...
Tópico(s): Stress Responses and Cortisol
2001 - Oxford University Press | International Immunology
Michael J. Ausserlechner, Petra Obexer, G. Jan Wiegers, Bernd Hartmann, Stephan Geley, Reinhard Kofler,
The cyclin-dependent kinase inhibitor p16INK4A is frequently inactivated in childhood T-cell acute lymphoblastic leukemia. To investigate possible consequences of this genetic alteration for tumor development, we conditionally expressed p16INK4A in the T-cell acute lymphoblastic leukemia line CCRF-CEM, which carries a homozygous deletion of this gene. In agreement with its reported function, p16INK4A expression was associated with hypophosphorylation of the retinoblastoma protein pRB and stable ...
Tópico(s): Retinoids in leukemia and cellular processes
2001 - Elsevier BV | Journal of Biological Chemistry
G. Jan Wiegers, Ilona E. M. Stec, Wolfgang E. F. Klinkert, Astrid C. E. Linthorst, Johannes M. H. M. Reul,
Glucocorticoids inhibit stimulus-induced T-cell proliferation, an early and essential parameter of cellular immunity. It was recently found however that physiological concentrations of glucocorticoids can also accelerate, not only inhibit, rat T-cell mitogenesis. We investigated mechanism(s) underlying mitogenic actions of glucocorticoids on anti-T-cell receptor (TCR)- and concanavalin A (Con A)-induced T-cell proliferation. Surprisingly, the ability of the glucocorticoid corticosterone (CORT) to either ...
Tópico(s): Immune Response and Inflammation
2001 - Karger Publishers | Neuroendocrinology
G. Jan Wiegers, Michael Knoflach, Günther Böck, Harald Niederegger, Hermann Dietrich, András Falus, Richard L. Boyd, Georg Wick,
While signaling by either the TCR or glucocorticoid receptor (GR) can induce apoptosis in thymocytes, recent studies have shown that combining these signals results in survival of CD4+CD8+ thymocytes. Although glucocorticoids (GC) in this way may directly affect T cell selection, no data are available addressing GR expression in thymocyte subsets and in individual cellswithin subsets. We studied GR expression by combining immunofluorescence cell surface staining for CD4, CD8 and TCR with intracellular ...
Tópico(s): Immunotherapy and Immune Responses
2001 - Wiley | European Journal of Immunology
Wieger Tiddens, Jan Braaksma, Tiedo Tinga,
Progresses in prognostic maintenance technologies offer opportunities to aid the asset owner in optimal maintenance and life cycle decision making, e.g. replacement or life-time extension of physical assets. Using accurate lifetime predictions is critical for ensuring just-in-time maintenance. Although there is considerable literature on specific techniques, reports on the adoption and usage of these methods show that only a small amount of companies have applied these techniques. This study therefore ...
Tópico(s): Machine Fault Diagnosis Techniques
2015 - Elsevier BV | Procedia CIRP
Johannes M. H. M. Reul, Marta Labeur, G. Jan Wiegers, Astrid C. E. Linthorst,
Presently, it is clear that the brain, immune system, and endocrine system build a complex network of interactions at various levels. Inflammation, which may be regarded as a stressful challenge, initiates apart from immunological, autonomic, and neuroendocrine responses also profound behavioral (e.g., immobility, social disinterest) changes. Key mediators herein are corticotropin-releasing hormone (CRH) and cytokines, such as interleukin-1 beta (IL-1 beta). Currently, the behavioral changes, collectively ...
Tópico(s): Neuroinflammation and Neurodegeneration Mechanisms
1998 - Wiley | Annals of the New York Academy of Sciences
Lena Müller, Daniela Hainberger, Valentina Stolz, Patricia Hamminger, Hammad Hassan, Teresa Preglej, Nicole Boucheron, Shinya Sakaguchi, G. Jan Wiegers, Andreas Villunger, Johan Auwerx, Wilfried Ellmeier,
Nuclear receptor corepressor 1 (NCOR1) is a transcriptional regulator bridging repressive chromatin modifying enzymes with transcription factors. NCOR1 regulates many biological processes, however its role in T cells is not known. Here we show that Cd4-Cre-mediated deletion of NCOR1 (NCOR1 cKO
Tópico(s): Toxin Mechanisms and Immunotoxins
2017 - Nature Portfolio | Scientific Reports
Lourdes Rocamora‐Reverte, Holger M. Reichardt, Andreas Villunger, G. Jan Wiegers,
Glucocorticoids (GC) are essential regulators of T-cell development and function. Activation of the immune system increases systemic adrenal-derived GC levels which downregulate immune activity as part of a negative feedback control system. Increasing evidence shows, however, that GC can also be derived from extra-adrenal sources such as the thymus or intestine, thus providing local control of GC-mediated effects. The thymus reportedly produces GC, but whether thymic epithelial cells or thymocytes ...
Tópico(s): Adrenal Hormones and Disorders
2017 - Springer Nature | Cell Death and Disease