Christina C. Roggatz, Mercedes González‐Wangüemert, Hugo Pereira, Maria João Rodrigues, Manuela Moreira da Silva, Luísa Barreira, João Varela, Luísa Custódio,
This work reports for the first time the nutritional profile and antioxidant potential of the edible sea cucumber Holothuria arguinensis from the North-eastern Atlantic. H. arguinensis has high levels of protein, with the amino acids profile dominated by alanine, glycine and proline and low lysine/arginine ratios. Its carbohydrate and energetic contents are also low as well as the total lipid levels, although its lipid profile is rich in polyunsaturated fatty acids (PUFA), especially arachidonic, ...
Tópico(s): Marine Sponges and Natural Products
2015 - Taylor & Francis | Natural Product Research
Andreia Lopes, Maria João Rodrigues, Catarina Pereira, Marta Oliveira, Luísa Barreira, João Varela, Francesca Trampetti, Luísa Custódio,
Extremophile plants, halophytes in particular, still remain largely unexplored and underutilized despite their high potential as source of industrial raw materials like natural antioxidants in industries related to pharmaceutical, cosmetics and functional ingredients. The goal of this study was to explore the phenolic content and the in vitro antioxidant and tyrosine inhibition properties of polyphenol-rich extracts of twenty-one extremophile plant species collected in saline habitats of the South ...
Tópico(s): Natural product bioactivities and synthesis
2016 - Elsevier BV | Industrial Crops and Products
Luísa Custódio, Laura Silvestre, Maria Isabel Rocha, Maria João Rodrigues, Catarina Vizetto‐Duarte, Hugo Pereira, Luísa Barreira, João Varela,
Context Marine macroalgae contain several bioactive molecules that may be developed as functional foods, but information about their neuroprotective potential is scarce.Objective The objective of this study is to determine the in vitro antioxidant and neuroprotective features of marine algae from the southern coast of Portugal and to assess the total content of different types of bioactives.Materials and methods Methanol extracts from 21 macroalgal species from the southern Portugal were evaluated ...
Tópico(s): Free Radicals and Antioxidants
2016 - Taylor & Francis | Pharmaceutical Biology
Katkam N. Gangadhar, Maria João Rodrigues, Hugo Pereira, Helena Gaspar, F. Xavier Malcata, Luísa Barreira, João Varela,
Tisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extracts exhibited in vitro cytotoxic activity against human hepatocarcinoma (HepG2) cells. However, so far, the compound(s) accountable for the observed bioactivity ...
Tópico(s): Marine Sponges and Natural Products
2020 - Multidisciplinary Digital Publishing Institute | Marine Drugs
Produção Nacional
Revisado por pares
Alex Cabral, Yvonne Y. Y. Yau, Gloria Reithmaier, Luiz C. Cotovicz, João Barreira, Göran Broström, Bárbara Viana, Alessandra Fonseca, Isaac R. Santos,
Mangrove soils are highly enriched in organic carbon. Tidal pumping drives seawater and oxygen into mangrove soils during flood tide and releases carbon-rich porewater during ebb tides. Here, we resolve semi-diurnal (flood/ebb tides), diel (day/night) and weekly (neap/spring tides) drivers of porewater-derived CO2 fluxes in two mangroves and update global estimates of CO2 emissions building on earlier observations from other sites. Tidal pumping controlled pCO2 variability within the two mangrove creeks. ...
Tópico(s): Geology and Paleoclimatology Research
2024 - Elsevier BV | Geochimica et Cosmochimica Acta
Carlos Cardoso, Hugo Pereira, João M. P. França, Joana Matos, Ivo Monteiro, Pedro Pousão‐Ferreira, A. Gomes, Luísa Barreira, João Varela, Nuno R. Neng, J.M.F. Nogueira, Cláudia Afonso, Narcisa M. Bandarra,
Tópico(s): Antioxidant Activity and Oxidative Stress
2019 - Springer Science+Business Media | Aquaculture International
Marina Baessa, Maria João Rodrigues, Catarina Pereira, Tamára Santos, Nuno R. Neng, J.M.F. Nogueira, Luísa Barreira, João Varela, Haroon Ahmed, Saira Asif, Sofiene Boukhari, Waqas Khan Kayani, Khawaja Shafique Ahmad, Gökhan Zengin, Adriano Mollica, Luísa Custódio,
Infusions, decoctions and tinctures were prepared from flowers of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret and evaluated for in vitro inhibition of enzymes implicated on the onset of neurological diseases (acetylcholinesterase: AChE and butyrylcholinesterase: BuChE), diabetes (α-glucosidase and α-amylase), obesity (lipase) and skin hyperpigmentation (tyrosinase). Extracts were also appraised for radical scavenging activity (RSA) on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- ...
Tópico(s): Cholinesterase and Neurodegenerative Diseases
2018 - Elsevier BV | South African Journal of Botany
Maria João Rodrigues, Katkam N. Gangadhar, Catarina Vizetto‐Duarte, Sileshi Gizachew Wubshet, Nils T. Nyberg, Luísa Barreira, João Varela, Luísa Custódio,
Extracts of five halophytes from southern Portugal (Arthrocnemum macrostachyum, Mesembryanthemum edule, Juncus acutus, Plantago coronopus and Halimione portulacoides), were studied for antioxidant, anti-inflammatory and in vitro antitumor properties. The most active extracts towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were the methanol extracts of M. edule (IC50 = 0.1 mg/mL) and J. acutus (IC50 = 0.4 mg/mL), and the ether extracts of J. acutus (IC50 = 0.2 mg/mL) and A. macrostachyum (IC50 = 0.3 mg/ ...
Tópico(s): Phytochemical Studies and Bioactivities
2014 - Multidisciplinary Digital Publishing Institute | Marine Drugs
Sofia Barreira, João Eurico Fonseca,
Tópico(s): Rheumatoid Arthritis Research and Therapies
2016 - Springer Nature | Clinical Reviews in Allergy & Immunology
Produção Nacional
Revisado por pares
Júlia Barreira, João Guilherme Cren Chiminazzo, Paula Teixeira Fernandes,
The aim of this study was to analyze the point difference established at different phases of the game by the winners and losers in men's singles badminton matches. We analyzed 136 games from matches of the 2015 World Championship. From each game were collected the final result and the maximum point difference established by the players in each phase of the game. We considered from 0 to 7 points the first phase, from 8 to 14 the second phase and 15 to 21 the third phase of the game. We found that ...
Tópico(s): Sports Dynamics and Biomechanics
2016 - Taylor & Francis | International Journal of Performance Analysis in Sport
Patrícia Pereira, Maria Cristina Roque‐Barreira, João A. Queiroz, Francisco Veiga, Fani Sousa, Ana Figueiras,
Recent scientific discoveries have revealed the potential of using RNA molecules as therapeutic agents/targets. However, a significant key factor in the success of RNA-based therapeutics is the development of vehicles that allow their efficient delivery in the correct dose, time and location, without causing unwanted side effects. Areas covered: In this review, we provide an overview of the recent approaches proposed to overcome the chemical, biochemical and physiological barriers still present ...
Tópico(s): DNA and Nucleic Acid Chemistry
2016 - Taylor & Francis | Expert Opinion on Drug Delivery
Catarina Vizetto‐Duarte, Hugo Pereira, Carolina Bruno de Sousa, Amélia P. Rauter, Fernando Alberício, Luísa Custódio, Luísa Barreira, João Varela,
AbstractThe fatty acid (FA) composition of six macroalgae from the Cystoseira genus, namely Cystoseira compressa, Cystoseira humilis, Cystoseira tamariscifolia, Cystoseira nodicaulis, Cystoseira baccata and Cystoseira barbata, was determined. Polyunsaturated fatty acids (PUFA) corresponded to 29–46% of the total FA detected. C. compressa, C. tamariscifolia and C. nodicaulis stood out for their low PUFA/saturated fatty acid, low n-6 PUFA/n-3 PUFA ratios as well as favourable unsaturation, atherogenicity ...
Tópico(s): Algal biology and biofuel production
2015 - Taylor & Francis | Natural Product Research
Lisa Schüler, Gabriel Bombo, Paulo Duarte, Tamára Santos, Inês B. Maia, Filipa Pinheiro, José Marques, Rita Jacinto, Peter S.C. Schulze, Hugo Pereira, Luísa Barreira, João Varela,
In this study, two carotenoid-rich strains of the euryhaline microalga Tetraselmis striata CTP4 were isolated by random mutagenesis combined with selection via fluorescence activated cell sorting and growth on norflurazon. Both strains, ED5 and B11, showed an up to 1.5-fold increase in carotenoid contents as compared with the wildtype, independent of the growth conditions. More specifically, violaxanthin, β-carotene and lutein contents reached as high as 1.63, 4.20 and 3.81 mg g−1 DW, respectively. ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2021 - Elsevier BV | Bioresource Technology
Lisa Schüler, Tamára Santos, Hugo Pereira, Paulo Duarte, Katkam N. Gangadhar, Cláudia Florindo, Peter S.C. Schulze, Luísa Barreira, João Varela,
The industrial microalga Tetraselmis sp. CTP4 is a promising candidate for aquaculture feed, novel food, cosmeceutical and nutraceutical due to its balanced biochemical profile. To further upgrade its biomass value, carotenogenesis was investigated by testing four environmental factors, namely temperature, light intensity, salinity and nutrient availability over different growth stages. The most important factor for carotenoid induction in this species is a sufficient supply of nitrates leading ...
Tópico(s): Physiological and biochemical adaptations
2019 - Elsevier BV | Algal Research
Hugo Pereira, Joana Silva, Tamára Santos, Katkam N. Gangadhar, Ana Raposo, Cláudia Nunes, Manuel A. Coimbra, Luísa Gouveia, Luísa Barreira, João Varela,
Commercial production of microalgal biomass for food and feed is a recent worldwide trend. Although it is common to publish nutritional data for microalgae grown at the lab-scale, data about industrial strains cultivated in an industrial setting are scarce in the literature. Thus, here we present the nutritional composition and a microbiological and toxicological evaluation of Tetraselmis sp. CTP4 biomass, cultivated in 100-m3 photobioreactors at an industrial production facility (AlgaFarm). This ...
Tópico(s): Aquaculture Nutrition and Growth
2019 - Multidisciplinary Digital Publishing Institute | Molecules
Mafalda Trovão, Hugo Pereira, Joana Silva, Jaime Páramo, Pedro Quelhas, Tamára Santos, Joana T. Silva, Adriana Rodrigues Machado, Luísa Gouveia, Luísa Barreira, João Varela,
Biomass harvesting is one of the most expensive steps of the whole microalgal production pipeline. Therefore, the present work aimed to understand the effect of salinity on the growth performance, biochemical composition and sedimentation velocity of Tetraselmis sp. CTP4, in order to establish an effective low-cost pilot-scale harvesting system for this strain. At lab scale, similar growth performance was obtained in cultures grown at salinities of 5, 10 and 20 g L-1 NaCl. In addition, identical ...
Tópico(s): Algal biology and biofuel production
2019 - Elsevier BV | Heliyon
Hugo Pereira, Peter S.C. Schulze, Lisa Schüler, Tamára Santos, Luísa Barreira, João Varela,
Microalgal biotechnology has gained increasing attention over the last few decades as a next-generation driver for obtaining food, feed and biofuels and to carry out bioremediation of effluents and CO2 mitigation. Flow cytometry (FC) and fluorescence-activated cell sorting (FACS) have recently acquired outstanding importance in the development of high-throughput methodologies. For example, bioprospecting novel species using FACS widened the current portfolio of available strains for drug discovery ...
Tópico(s): Marine and coastal ecosystems
2018 - Elsevier BV | Algal Research
Hugo Pereira, Jaime Páramo, Joana Silva, Ana Evangelista Marques, Ana Barros, Dinis Maurício, Tamára Santos, Peter S.C. Schulze, Raúl J. Barros, Luísa Gouveia, Luísa Barreira, João Varela,
Abstract Industrial production of novel microalgal isolates is key to improving the current portfolio of available strains that are able to grow in large-scale production systems for different biotechnological applications, including carbon mitigation. In this context, Tetraselmis sp. CTP4 was successfully scaled up from an agar plate to 35- and 100-m 3 industrial scale tubular photobioreactors (PBR). Growth was performed semi-continuously for 60 days in the autumn-winter season (17 th October – 14 ...
Tópico(s): Biocrusts and Microbial Ecology
2018 - Nature Portfolio | Scientific Reports
Vitor E.M. Cardoso, Nuno M.M. Ramos, Ricardo M. S. F. Almeida, Eva Barreira, João Poças Martins, M. Lurdes Simões, Luís Sanhudo,
Transport stations are distributive hubs composed of transient spaces, often not fully indoor, where most users spend time waiting to travel or waiting for travelers. The aim of this article is to present a comparison between thermal comfort evaluation methods applied in a free running bus terminal located in a mild climate country. Data was collected in field measurements and surveys were performed on 240 passengers, focusing warm season operation conditions. The collected information allowed for ...
Tópico(s): Urban Heat Island Mitigation
2018 - Elsevier BV | Energy and Buildings
Miguel A. Hernández‐Prieto, Verena Schön, Jens Georg, Luísa Barreira, João Varela, Wolfgang R. Hess, Matthias E. Futschik,
Abstract Iron is an essential cofactor in many metabolic reactions. Mechanisms controlling iron homeostasis need to respond rapidly to changes in extracellular conditions, but they must also keep the concentration of intracellular iron under strict control to avoid the generation of damaging reactive oxygen species. Due to its role as a redox carrier in photosynthesis, the iron quota in cyanobacteria is about 10 times higher than in model enterobacteria. The molecular details of how such a high ...
Tópico(s): Aquatic Ecosystems and Phytoplankton Dynamics
2012 - Genetics Society of America | G3 Genes Genomes Genetics
Eva Barreira, João M. P. Q. Delgado, Nuno M.M. Ramos, Vasco Peixoto de Freitas,
This article intends to apply existing numerical models of exterior boundary conditions on the simulation of exterior condensation on façades (undercooling phenomenon) finished with external thermal insulation composite systems. The results of three hygrothermal models were compared, regarding the temperature on the exterior surface of a west façade. The climatic conditions from Porto city were used. We analysed in detail how the simulation of the undercooling phenomenon is influenced by the numerical ...
Tópico(s): Wind and Air Flow Studies
2011 - Taylor & Francis | Journal of Building Performance Simulation
Produção Nacional
Revisado por pares
Ademílson Panunto-Castelo, Mayara Andrade Souza, Maria Cristina Roque‐Barreira, João S. Silva,
The outcome and severity of some diseases correlate with the dominance of either the T helper 1 (Th1) or Th2 immune response, which is stimulated by IL-12 or IL-4, respectively. In the present study we demonstrate that gamma interferon (IFN-gamma) secretion by murine spleen cells stimulated with KM(+), a mannose-binding lectin from Artocarpus integrifolia, is due to IL-12 induction, because (1) macrophages from several sources (including cell lines) produced IL-12 p40 in response to KM(+), and (2) lectin- ...
Tópico(s): Immune Cell Function and Interaction
2001 - Oxford University Press | Glycobiology
Produção Nacional
Revisado por pares
Miguel–Ángel Gómez, Adrián Cid, Fernando Rivas, Júlia Barreira, João Guilherme Cren Chiminazzo, Jaime Prieto,
The scoring performance in badminton can reveal important stages during the match and different characteristics according to notational, temporal and contextual-related variables. The aim of the present study was to: (i) analyse the scoring performance dynamics of men's badminton players according to tournament stage (group stage, knockout stage), game number (game 1, game 2, game 3) and game half (before and after the mid-game break when the leading player reaches 11 points); (ii) identify the persistency ...
Tópico(s): Sports Dynamics and Biomechanics
2021 - Elsevier BV | Chaos Solitons & Fractals
Ana Raquel Leal, Lillian Barros, João C.M. Barreira, Maria João Sousa, Anabela Martins, Celestino Santos‐Buelga, Isabel C.F.R. Ferreira,
The search for foods that might improve health or reduce disease risk, has been progressively gaining interest. Mushroom could be examples of these foods, presenting the additional advantage of being recognized as a delicacy. This feature might place mushrooms in the pharma–nutrition interface. Herein, eight different mushroom species were characterized in terms of nutrients (proteins, carbohydrates, fat, individual sugars, fatty acids) and bioactive compounds (tocopherols, carotenoids, organic acids ...
Tópico(s): Polysaccharides and Plant Cell Walls
2012 - Elsevier BV | Food Research International
Rafaela Guimarães, Lillian Barros, João C.M. Barreira, Ma João Sousa, Ana Maria Carvalho, Isabel C.F.R. Ferreira,
A comparative study between the antioxidant properties of peel (flavedo and albedo) and juice of some commercially grown citrus fruit (Rutaceae), grapefruit (Citrus paradisi), lemon (Citrus limon), lime (Citrus × aurantiifolia) and sweet orange (Citrus sinensis) was performed. Different in vitro assays were applied to the volatile and polar fractions of peels and to crude and polar fraction of juices: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, reducing power and inhibition of lipid ...
Tópico(s): Essential Oils and Antimicrobial Activity
2009 - Elsevier BV | Food and Chemical Toxicology
Vitor E.M. Cardoso, Nuno M.M. Ramos, Ricardo M. S. F. Almeida, Eva Barreira, João Poças Martins, M. Lurdes Simões, Luís Sanhudo, Bruno Ribeiro,
The variations of building typologies contribute to the difficulty of performing a correct analysis of the comfort conditions in buildings that do not fit the more common geometries and occupation patterns. The main objective of the article is to evaluate the comfort conditions of cruise terminal buildings, an example of this type of problem. A twofold strategy, comprising in-situ measurements and user surveys was implemented. A total of 20 independent field measurements of thermal comfort parameters ...
Tópico(s): Wind and Air Flow Studies
2017 - Elsevier BV | Building and Environment
Mafalda Costa, Pedro Barrulas, Luís Dias, Maria da Conceição Lopes, João Barreira, Bernard Clist, Karlis Karklins, Maria da Piedade de Jesus, Sónia da Silva Domingos, Peter Vandenabeele, José Mirão,
The glass bead assemblages recovered during the 2014 excavations of the ruins of Kulumbimbi located in Mbanza Kongo (Angola) were analyzed by means of a multi-analytical minimally invasive methodology, which includes handheld X-ray fluorescence (hXRF), variable pressure scanning electron microscope coupled with energy dispersive X-ray spectrometry (VP-SEM-EDS), micro-Raman spectroscopy and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Chemical data indicate that cobalt, copper, ...
Tópico(s): Archaeology and ancient environmental studies
2019 - Elsevier BV | Microchemical Journal
Vera L. Maria, Tânia Gomes, Luísa Barreira, Maria João Bebianno,
In natural waters, chemical interactions between mixtures of contaminants can result in potential synergistic and/or antagonic effects in aquatic animals. Benzo(a)pyrene (BaP) and copper (Cu) are two widespread environmental contaminants with known toxicity towards mussels Mytilus spp. The effects of the individual and the interaction of BaP and Cu exposures were assessed in mussels Mytilus galloprovincialis using proteomic analysis. Mussels were exposed to BaP [10 μg L−1 (0.396 μM)], and Cu [10 μg L− ...
Tópico(s): thermodynamics and calorimetric analyses
2013 - Elsevier BV | Aquatic Toxicology
Maria João Barreira, Paula Alvito, Cristina Almeida,
An analytic procedure based on high-performance liquid chromatography with solid-phase extraction and UV detection (SPE–HPLC–UV) was validated and used to analyse 144 apple-based-foods, including 68 apple juices (32 clear and 36 cloudy) and 76 homogenised apple purees marketed in Portugal. Patulin was detected in 33 samples (23%) with values ranging from 1.2 μg/kg to 42 μg/kg. Patulin was not detected in the analysed infant drinks but its presence was quantified in five homogenised apple puree (7%) ...
Tópico(s): Wheat and Barley Genetics and Pathology
2010 - Elsevier BV | Food Chemistry
Produção Nacional
Revisado por pares
Selene Maia de Morais, Valdir Alves Facundo, Luciana Medeiros Bertini, Eveline Solon Barreira Cavalcanti, João Francisco dos Anjos Júnior, Silane Aparecida Ferreira, Edy Sousa de Brito, M. A. de Souza Neto,
The larvicidal activity of essential oils of four species of Piper from the Amazon Forest was tested using third-instar larvae of Aedes aegypti. The oils were extracted by steam distillation and analyzed by GC and GC–MS. The main components isolated from each Piper species were as follows: viridiflorol (27.50%), aromadendrene (15.55%) and β-selinene (10.50%) from Piper gaudichaudianum; β-selinene (15.77%) and caryophyllene oxide (16.63%) from Piper humaytanum; dillapiol (54.70%) and myristicin (25.61%) ...
Tópico(s): Mosquito-borne diseases and control
2007 - Elsevier BV | Biochemical Systematics and Ecology