Tomás P. Miguel, Santiago Pavón, Joaquín Salváchua, Juan Quemada, Pedro Luís Chas Alonso, Javier Fernandez-Amigo, Carlos Alberto Acuña, Lidia Rodriguez Yamamoto, Vasco Lagarto, Joao Vastos,
Tópico(s): Service-Oriented Architecture and Web Services
1994 - Springer Science+Business Media | Lecture notes in computer science
João Lagarto, Jorge Sousa, Álvaro Martins, Paulo Ferrão,
In the last years the electricity industry has faced a restructuring process. Among the aims of this process was the increase in competition, especially in the generation activity where firms would have an incentive to become more efficient. However, the competitive behavior of generating firms might jeopardize the expected benefits of the electricity industry liberalization. The present paper proposes a conjectural variations model to study the competitive behavior of generating firms acting in ...
Tópico(s): Renewable energy and sustainable power systems
2014 - Elsevier BV | Energy
Hugo Algarvio, Fernando Lopes, Jorge Sousa, João Lagarto,
The major electricity market models include: pools, bilateral contracts and hybrid models. Pool prices tend to change quickly and variations are usually highly unpredictable. In this way, market participants can enter into bilateral contracts to hedge against pool price volatility. In bilateral contracts, market participants can set the terms and conditions of agreements independent of the market operator. The hybrid model combines features of both pools and bilateral contracts. This paper is devoted ...
Tópico(s): Energy Load and Power Forecasting
2017 - Elsevier BV | Electric Power Systems Research
Jorge Sousa, João Lagarto, Cristina Camus, Carla Viveiros, Filipe Barata, Pedro Silva, Ricardo Alegria, O. Paraíba,
The EU Renewable Energy Directive 2018/2001 (RED II) has unlocked the participation of local citizens and authorities in collective renewable energy projects through the concept of Renewable Energy Communities (REC). In this context, the present study proposes an optimization model to support REC's investment decisions on the renewable generation portfolio and operational electricity sharing management. The model presented follows an annualized investment optimization approach considering that one ...
Tópico(s): Energy and Environment Impacts
2023 - Elsevier BV | Energy
Jorge Sousa, João Lagarto, Ezequiel Carvalho, Ana Martins,
This paper presents the simulation platform SWHORD, specially designed for the analysis of future energy systems under energy transition targets. The model is implemented in GAMS as a cost minimization mixed integer programming problem of a hydro-thermal power system, which includes high penetration of non-dispatchable renewable generation, storage technologies, electric vehicles, and hydrogen systems. Simulations are performed on an hourly basis for one year of operation, enabling the evaluation ...
Tópico(s): Hybrid Renewable Energy Systems
2023 - Elsevier BV | Energy
Hugh B. Manning, Mohammad B. Nickdel, Kazuhiro Yamamoto, João L. Lagarto, Douglas J. Kelly, Clifford Talbot, Gordon T. Kennedy, Jayesh Dudhia, John Lever, Christopher Dunsby, P. M. W. French, Yoshifumi Itoh,
Cartilage is a vital organ to maintain joint function. Upon arthritis, proteolytic enzymes initiate degradation of cartilage extracellular matrix (ECM) resulting in eventual loss of joint function. However, there are only limited ways of non-invasively monitoring early chemical changes in cartilage matrix. Here we report that the autofluorescence decay profiles of cartilage tissue are significantly affected by proteolytic degradation of cartilage ECM and can be characterised by measurements of the ...
Tópico(s): Protease and Inhibitor Mechanisms
2012 - Elsevier BV | Matrix Biology
Clifford Talbot, João L. Lagarto, Sean Warren, Mark A. A. Neil, P. M. W. French, Chris Dunsby,
A correction is proposed to the Delta function convolution method (DFCM) for fitting a multiexponential decay model to time-resolved fluorescence decay data using a monoexponential reference fluorophore. A theoretical analysis of the discretised DFCM multiexponential decay function shows the presence an extra exponential decay term with the same lifetime as the reference fluorophore that we denote as the residual reference component. This extra decay component arises as a result of the discretised ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2015 - Springer Science+Business Media | Journal of Fluorescence
João L. Lagarto, Benjamin Dyer, Clifford B. Talbot, Nicholas S. Peters, P. M. W. French, Alexander R. Lyon, Chris Dunsby,
Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2018 - Optica Publishing Group | Biomedical Optics Express
João L. Lagarto, Vladislav I. Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi,
Abstract Time‐correlated single photon counting is the “gold‐standard” method for fluorescence lifetime measurements and has demonstrated potential for clinical deployment. However, the translation of the technology into clinic is hindered by the use of ultrasensitive detectors, which make the fluorescence acquisition impractical with bright lighting conditions such as in clinical settings. We address this limitation by interleaving periodic fluorescence detection with synchronous out‐of‐phase externally ...
Tópico(s): Photodynamic Therapy Research Studies
2019 - Wiley | Journal of Biophotonics
João L. Lagarto, Federica Villa, Simone Tisa, Franco Zappa, Vladislav I. Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi,
Abstract Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label-free contrast between normal and diseased tissues, both in vivo and ex vivo . We report the development of an instrument employing Single Photon Avalanche Diode (SPAD) arrays to realize real-time multispectral autofluorescence lifetime imaging at a macroscopic scale using handheld single-point fibre optic probes, under bright background conditions. At the detection end, the fluorescence signal is passed ...
Tópico(s): Photoacoustic and Ultrasonic Imaging
2020 - Nature Portfolio | Scientific Reports
João L. Lagarto, Mohammad B. Nickdel, Douglas J. Kelly, Andrew Price, Jagdeep Nanchahal, Chris Dunsby, P. M. W. French, Yoshifumi Itoh,
Abstract Osteoarthritis (OA) is the most common arthritis and its hallmark is degradation of articular cartilage by proteolytic enzymes leading to loss of joint function. It is challenging to monitor the status of cartilage in vivo and this study explores the use of autofluorescence lifetime (AFL) measurements to provide a label-free optical readout of cartilage degradation that could enable earlier detection and evaluation of potential therapies. We previously reported that treatment of ex vivo porcine ...
Tópico(s): Knee injuries and reconstruction techniques
2020 - Nature Portfolio | Scientific Reports
Jakob Unger, Christoph Hebisch, Jennifer E. Phipps, João L. Lagarto, Hanna Kim, Morgan Darrow, Richard J. Bold, Laura Marcu,
Tumor-free surgical margins are critical in breast-conserving surgery. In up to 38% of the cases, however, patients undergo a second surgery since malignant cells are found at the margins of the excised resection specimen. Thus, advanced imaging tools are needed to ensure clear margins at the time of surgery. The objective of this study was to evaluate a random forest classifier that makes use of parameters derived from point-scanning label-free fluorescence lifetime imaging (FLIm) measurements of ...
Tópico(s): Medical Imaging Techniques and Applications
2020 - Optica Publishing Group | Biomedical Optics Express
J. Almeida-Pinto, Matilde R. Lagarto, Pedro Lavrador, João F. Mano, Vítor M. Gaspar,
Breakthroughs in precision cell surface engineering tools are supporting the rapid development of programmable living assemblies with valuable features for tackling complex biological problems. Herein, the authors overview the most recent technological advances in chemically- and biologically-driven toolboxes for engineering mammalian cell surfaces and triggering their assembly into living architectures. A particular focus is given to surface engineering technologies for enabling biomimetic cell- ...
Tópico(s): Additive Manufacturing and 3D Printing Technologies
2023 - Wiley | Advanced Science
Taylor M. Cannon, João L. Lagarto, Benjamin Dyer, Edwin García, Douglas J. Kelly, Nicholas S. Peters, Alexander R. Lyon, P. M. W. French, Chris Dunsby,
Reduced nicotinamide adenine dinucleotide (NADH) is the principal electron donor in glycolysis and oxidative metabolism and is thus recognized as a key biomarker for probing metabolic state. While the fluorescence characteristics of NADH have been investigated extensively, there are discrepancies in the published data due to diverse experimental conditions, instrumentation and microenvironmental parameters that can affect NADH fluorescence. Using a cuvette-based time-resolved spectrofluorimeter employing ...
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2021 - Optica Publishing Group | OSA Continuum
Tanveer Ahmed Shaik, João L. Lagarto, Enrico Baria, Melis Goktas, Patrick Igoche Onoja, Kerstin G. Blank, Francesco S. Pavone, Jürgen Popp, Christoph Krafft, Riccardo Cicchi,
We demonstrate the ability of nondestructive optical imaging techniques such as second-harmonic generation (SHG), two-photon fluorescence (TPF), fluorescence lifetime imaging (FLIM), and Raman spectroscopy (RS) to monitor biochemical and mechanical alterations in tissues upon collagen degradation. Decellularized equine pericardium (EP) was treated with 50 μg/mL bacterial collagenase at 37 °C for 8, 16, 24, and 32 h. The SHG ratio (defined as the normalized ratio between SHG and TPF signals) remained unchanged ...
Tópico(s): Laser Applications in Dentistry and Medicine
2021 - American Chemical Society | Analytical Chemistry
João L. Lagarto, Benjamin Dyer, Clifford B. Talbot, Markus B. Sikkel, Nicholas S. Peters, P. M. W. French, Alexander R. Lyon, Chris Dunsby,
We investigate the potential of an instrument combining time-resolved spectrofluorometry and diffuse reflectance spectroscopy to measure structural and metabolic changes in cardiac tissue in vivo in a 16 week post-myocardial infarction heart failure model in rats. In the scar region, we observed changes in the fluorescence signal that can be explained by increased collagen content, which is in good agreement with histology. In areas remote from the scar tissue, we measured changes in the fluorescence ...
Tópico(s): Cardiovascular Function and Risk Factors
2015 - Optica Publishing Group | Biomedical Optics Express
Alberto Ignacio Herrando, Mireia Castillo-Martín, António Galzerano, Laura M. Fernández, Pedro Vieira, José Azevedo, Amjad Parvaiz, Riccardo Cicchi, Vladislav I. Shcheslavskiy, Pedro G. Silva, João L. Lagarto,
Advancements in optical imaging techniques have revolutionized the field of biomedical research, allowing for the comprehensive characterization of tissues and their underlying biological processes. Yet, there is still a lack of tools to provide quantitative and objective characterization of tissues that can aid clinical assessment in vivo to enhance diagnostic and therapeutic interventions. Here, we present a clinically viable fiber-based imaging system combining time-resolved spectrofluorimetry ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2023 - Optica Publishing Group | Biomedical Optics Express
Jorge Sousa, João Lagarto, Miguel Fonseca,
The energy transition, characterized by the decarbonization of energy consumption through electrification, needs a massive integration of non-dispatchable renewable electricity generation, along with storage systems and demand flexibility to manage the variability and uncertainty inherent of these sources. Within this framework, understanding the dynamic interaction among system resources becomes crucial to assess the adequacy of future electricity systems performance. This study aims to evaluate ...
Tópico(s): Smart Grid Energy Management
2024 - Elsevier BV | Renewable Energy
Alice Dal Fovo, Sara Mattana, A. A. Chaban, Diego Quintero Balbás, João L. Lagarto, Jana Striová, Riccardo Cicchi, Raffaella Fontana,
Fluorescence analysis of materials used as binders and coatings in artworks is often hampered by numerous factors, leading to uncertainties in data interpretation. Fluorescence lifetime (FL) measurements enable improvement of the specificity with respect to steady-state measurements by resolving the decay dynamics of the fluorophore emissions. In this work, layers of natural resin, oil, and wax—in pure form, pigmented, in mixtures, and spread on different substrates—were analyzed using a compact, ...
Tópico(s): Conservation Techniques and Studies
2021 - Multidisciplinary Digital Publishing Institute | Applied Sciences
Ana Martins, João Lagarto, H. Canacsinh, Francisco Reis, Margarida G. M. S. Cardoso,
Tópico(s): Stock Market Forecasting Methods
2022 - Springer Science+Business Media | Optimization and Engineering
João L. Lagarto, J. D. Hares, Christopher Dunsby, P. M. W. French,
Autofluorescence lifetime measurements, which can provide label-free readouts in biological tissues, contrasting e.g. different types and states of tissue matrix components and different cellular metabolites, may have significant clinical potential for diagnosis and to provide surgical guidance. However, the cost of the instrumentation typically used currently presents a barrier to wider implementation. We describe a low-cost single point time-resolved autofluorescence instrument, exploiting modulated ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2017 - Springer Science+Business Media | Journal of Fluorescence
João L. Lagarto, Jennifer E. Phipps, Leta M. Faller, Dinglong Ma, Jakob Unger, Julien Bec, Stephen M. Griffey, Jonathan Sorger, D. Gregory Farwell, Laura Marcu,
Tumor removal typically involves electrocautery, but no studies to date have quantified the effect of electrocautery on fluorescence emission. Electrocautery was applied to N=4 locations of the oral cavity and striated leg muscle of a live Yorkshire pig. Autofluorescence of cauterized tissues and surrounding regions was measured at distinct time points up to 120 minutes following cauterization. The fluorescence lifetime was spectrally resolved in four spectral detection channels that maximized the ...
Tópico(s): Laser Applications in Dentistry and Medicine
2018 - Elsevier BV | Journal of Photochemistry and Photobiology B Biology
João L. Lagarto, Benjamin Dyer, Nicholas S. Peters, P. M. W. French, Chris Dunsby, Alexander R. Lyon,
Cardiac remodeling following myocardial infarction (MI) involves structural and functional alterations in the infarcted and remote viable myocardium that can ultimately lead to heart failure. The underlying mechanisms are not fully understood and, following our previous study of the autofluorescence lifetime and diffuse reflectance signatures of the myocardium in vivo at 16 weeks post MI in rats [Biomed. Opt. Express6(2), 324 (2015)], we here present data obtained at 1, 2 and 4 weeks post myocardial ...
Tópico(s): Analytical Chemistry and Sensors
2019 - Optica Publishing Group | Biomedical Optics Express
João L. Lagarto, Vladislav I. Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi,
We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2020 - Optica Publishing Group | Optics Letters
João L. Lagarto, Caterina Credi, Federica Villa, Simone Tisa, Franco Zappa, Vladislav I. Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi,
Single Photon Avalanche Diode (SPAD) arrays are increasingly exploited and have demonstrated potential in biochemical and biomedical research, both for imaging and single-point spectroscopy applications. In this study, we explore the application of SPADs together with fiber-optic-based delivery and collection geometry to realize fast and simultaneous single-point time-, spectral-, and depth-resolved fluorescence measurements at 375 nm excitation light. Spectral information is encoded across the columns ...
Tópico(s): Photoacoustic and Ultrasonic Imaging
2019 - Multidisciplinary Digital Publishing Institute | Sensors
Sara Mattana, Alice Dal Fovo, João L. Lagarto, Maria Chiara Bossuto, Vladislav I. Shcheslavskiy, Raffaella Fontana, Riccardo Cicchi,
The non-invasive analysis of fluorescence from binders and pigments employed in mixtures in artworks is a major challenge in cultural heritage science due to the broad overlapping emission of different fluorescent species causing difficulties in the data interpretation. To improve the specificity of fluorescence measurements, we went beyond steady-state fluorescence measurements by resolving the fluorescence decay dynamics of the emitting species through time-resolved fluorescence imaging (TRFI). ...
Tópico(s): Building materials and conservation
2022 - Multidisciplinary Digital Publishing Institute | Molecules
... atribuição de Prémio da Crítica/APCT 2006 a João Lagarto, pela sua interpretação no espectáculo Começar a acabar, ...
Tópico(s): Cultural, Media, and Literary Studies
2007 - | Sinais de Cena
Douglas J. Kelly, Sean Warren, Sunil Kumar, João L. Lagarto, Benjamin Dyer, Anca Margineanu, Eric W.‐F. Lam, Chris Dunsby, P. M. W. French,
Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular autofluorescence originating from the coenzyme NADH in the context of investigating cell metabolic state. We present here an automated multiwell plate reading FLIM microscope optimized for UV illumination with the goal of extending high content fluorescence lifetime assays to readouts of metabolism. We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues ...
Tópico(s): Single-cell and spatial transcriptomics
2014 - World Scientific | Journal of Innovative Optical Health Sciences
Tanveer Ahmed Shaik, Enrico Baria, Xinyue Wang, Florian Korinth, João L. Lagarto, Christiane Höppener, Francesco S. Pavone, Volker Deckert, Jürgen Popp, Riccardo Cicchi, Christoph Krafft,
Tissue cross-linking represents an important and often used technique to enhance the mechanical properties of biomaterials. For the first time, we investigated biochemical and structural properties of genipin (GE) cross-linked equine pericardium (EP) using optical imaging techniques in tandem with quantitative atomic force microscopy (AFM). EP was cross-linked with GE at 37 °C, and its biochemical and biomechanical properties were observed at various time points up to 24 h. GE cross-linked EP was monitored ...
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2022 - American Chemical Society | Analytical Chemistry
Inês Farias, Elsa Couto, Neide Lagarto, João Delgado, Adelino V. M. Canário, Ivone Figueiredo,
Black scabbardfish, Aphanopus carbo, is a commercially important species that takes distant migrations throughout its life cycle. Sex steroids were measured by radioimmunoassay in the blood plasma of specimens caught off the Madeira Archipelago and mainland Portugal to link this species migratory path with its reproductive cycle. Furthermore, a pilot study using Mozambique tilapia (Oreochromis mossambicus) was designed to evaluate the effect of sample freshness on steroid levels because black scabbardfish ...
Tópico(s): Aquaculture Nutrition and Growth
2020 - Elsevier BV | Aquaculture and Fisheries