Produção Nacional
Revisado por pares
Maria Bellio, Ana Carolina Oliveira, Cláudia Mermelstein, Márcia Alves Marques Capella, João P. B. Viola, Jean‐Pierre Levraud, George A. DosReis, José O. Previato, Lúcia Mendonça‐Previato,
... for more papers by this authorJoão P. B. Viola, João P. B. Viola Programa de Medicina Experimental, Instituto ... for more papers by this authorJoão P. B. Viola, João P. B. Viola Programa de Medicina Experimental, Instituto Nacional do Cancer, ...
Tópico(s): Research on Leishmaniasis Studies
1999 - Wiley | The FASEB Journal
Produção Nacional
Revisado por pares
Flávia Gomes Martinez, Erica E.S. Hermel, Léder Leal Xavier, Giordano Gübert Viola, João Riboldi, Alberto A. Rasia‐Filho, Matilde Achaval,
The medial amygdala (MeA) is a sexually dimorphic area that modulates neuroendocrine and behavioral activities and where gonadal hormones play an important role in neuron–glial and synaptic plasticity. Immunohistochemistry was used to identify the astrocytic marker glial fibrillary acidic protein (GFAP) in the different MeA subnuclei – anterodorsal (MeAD), posterodorsal (MePD) and posteroventral (MePV) – of intact female rats in the different phases of the estrous cycle and in ovariectomized females treated ...
Tópico(s): Hypothalamic control of reproductive hormones
2006 - Elsevier BV | Brain Research
Produção Nacional
Revisado por pares
Mônica Ryff Moreira Roca Vianna, Mariana Alonso, Haydée Viola, João Quevedo, Fernanda de Paris, Melina Furman, Miguelina Levi de Stein, Jorge H. Medina, Iván Izquierdo,
Long-term habituation to a novel environment is one of the most elementary forms of nonassociative learning. Here we studied the effect of pre- or posttraining intrahippocampal administration of drugs acting on specific molecular targets on the retention of habituation to a 5-min exposure to an open field measured 24 h later. We also determined whether the exposure to a novel environment resulted in the activation of the same intracellular signaling cascades previously shown to be activated during ...
Tópico(s): Neuroinflammation and Neurodegeneration Mechanisms
2000 - Cold Spring Harbor Laboratory Press | Learning & Memory
Hélder Fraga, Joaquim G. Pinto, Francesco Viola, João A. Santos,
Abstract The olive tree is one of the most important crops in the Mediterranean basin. Given the strong climatic influence on olive trees, it becomes imperative to assess climate change impacts on this crop. Herein, these impacts were innovatively assessed, based on an ensemble of state‐of‐the‐art climate models, future scenarios and dynamic crop models. The recent‐past (1989–2005) and future (2041–2070, RCP4.5 and RCP8.5) olive growing season length (GSL), yield, growing season temperature (GST) and ...
Tópico(s): Plant Physiology and Cultivation Studies
2019 - Wiley | International Journal of Climatology
Giovanna Ponte, Paul Andrews, Viola Galligioni, João Pereira, Graziano Fiorito,
Recent increased interest in the welfare of cephalopods may be mainly due to their inclusion in Directive 2010/63/EU, which regulates—in Member States of the European Union—the use of animals for scientific research and educational purposes. Here we aim to challenge this view by providing information and considerations that have a broader impact than the use of these animals in an experimental context. We aim to provide a discussion around arguments linked to cephalopods’ welfare. We (1) discuss ...
Tópico(s): Meat and Animal Product Quality
2019 - Springer International Publishing | Animal welfare
Produção Nacional
Revisado por pares
Giordano Gübert Viola, Letícia Rodrigues, João C. Américo, Gisele Hansel, Rafael S. Vargas, Regina Biasibetti, Alessandra Swarowsky, Carlos Alberto Gonçalves, Léder Leal Xavier, Matilde Achaval, Diogo O. Souza, Olavo B. Amaral,
Environmental enrichment is known to induce plastic changes in the brain, including morphological changes in hippocampal neurons, with increases in synaptic and spine densities. In recent years, the evidence for a role of astrocytes in regulating synaptic transmission and plasticity has increased, and it is likely that morphological and functional changes in astrocytes play an important role in brain plasticity. Our study was designed to evaluate changes in astrocytes induced by environmental enrichment ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2009 - Elsevier BV | Brain Research
David Shinar, Pedro Valero‐Mora, Maura van Strijp-Houtenbos, Narelle Haworth, Amy Schramm, Guido De Bruyne, Viola Cavallo, Joannes Chliaoutakis, João P. Dias, Ottavia Eleonora Ferraro, Aslak Fyhri, Anica Hursa Šajatović, Kalev Kuklane, Rubén Daniel Ledesma, Óscar Calvé Mascarell, Anna Morandi, Martin H. Müser, Dietmar Otte, Maria Papadakaki, Jaime Sanmartín, Diana Dulf, Meltem Saplıoğlu, Georgia Tzamalouka,
Police crash reports are often the main source for official data in many countries. However, with the exception of fatal crashes, crashes are often underreported in a biased manner. Consequently, the countermeasures adopted according to them may be inefficient. In the case of bicycle crashes, this bias is most acute and it probably varies across countries, with some of them being more prone to reporting accidents to police than others. Assessing if this bias occurs and the size of it can be of great ...
Tópico(s): Urban Transport and Accessibility
2017 - Elsevier BV | Accident Analysis & Prevention
Produção Nacional
Revisado por pares
Thiago Wendt Viola, João Paulo Otolia Niederauer, Bruno Kluwe‐Schiavon, Breno Sanvicente‐Vieira, Rodrigo Grassi‐Oliveira,
Chronic cocaine use is associated with cognitive deficits, including poor performance on neuropsychological tasks of memory, executive functions, theory of mind and decision-making. However, the relationship between cocaine use disorder and social decision-making remains unclear. This is particularly relevant given the fact that many cocaine abusers present impairments in social functioning. In this sense, game theory paradigms have been helping to comprehend the behavior of psychiatric patients ...
Tópico(s): Neural and Behavioral Psychology Studies
2019 - BioMed Central | BMC Psychiatry
Produção Nacional
Revisado por pares
Hércules Moura, O. Fernandes, João P. B. Viola, S. P. Silva, R. H. Passos, Dirce Bonfim de Lima,
The occurrence of intestinal parasites, its relation with the transmission mechanism of HIV, and the clinical state of the AIDS patients, were analyzed in 99 Group IV patients (CDC, 1986), treated at "Hospital Universitário Pedro Ernesto" (HUPE), between 1986 and 1988. The group consisted of 79 (79.8%) patients whose HIV transmission mechanism took place through sexual contact and of 16 (20.2%) who were infected through blood. Feces samples from each patient were examined by four distincts methods (Faust ...
Tópico(s): Parasites and Host Interactions
1989 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Produção Nacional
Revisado por pares
Mauricio S. Caetano, Adriana Vieira‐de‐Abreu, Leonardo K. Teixeira, Miriam B. F. Werneck, Marcello A. Barcinski, João P. B. Viola,
Upon antigen stimulation, lymphocytes enter in cell cycle and proliferate, and most of the activated T cells die by apoptosis. Many of the proteins that regulate lymphocyte activation are Under the control of transcription factors belonging to the NFAT family. As previously demonstrated, NFATC2-/- mice consistently showed a marked increase in lymphocyte proliferation. Here, we evaluate the role of NFATC2 in regulating lymphocyte proliferation and its involvement in the control of cell cycle progression ...
Tópico(s): Calpain Protease Function and Regulation
2002 - Wiley | The FASEB Journal
Produção Nacional
Revisado por pares
Júlio Aliberti, João P. B. Viola, Adriana Vieira‐de‐Abreu, Patrı́cia T. Bozza, Alan Sher, Júlio Scharfstein,
Abstract Dendritic cells play a major role in the induction of both innate and acquired immune responses against pathogenic invaders. These cells are also able to sense endogenous activation signals liberated by injured tissues even in the absence of infection. In the present work, we demonstrate that kinins mobilize dendritic cells to produce IL-12 through activation of the B2 bradykinin receptor subtype and that bradykinin-induced IL-12 responses are tightly regulated both by angiotensin-converting ...
Tópico(s): Complement system in diseases
2003 - American Association of Immunologists | The Journal of Immunology
Produção Nacional
Revisado por pares
Maria Theresa de Souza Accioly, Patrícia Pacheco, Clarissa M. Maya‐Monteiro, Nina Carrossini, Bruno Kaufmann Robbs, Sílvia Silva de Oliveira, Cristiane Kaufmann, José Andrés Morgado‐Díaz, Patrı́cia T. Bozza, João P. B. Viola,
Lipid bodies (lipid droplets) are emerging as dynamic organelles involved in lipid metabolism and inflammation. Increased lipid body numbers have been described in tumor cells; however, its functional significance in cancer has never been addressed. Here, we showed increased number of lipid bodies in tumor tissues from patients with adenocarcinoma of colon submitted to surgical resection when compared with an adjacent normal tissue. Accordingly, increased numbers of lipid bodies were observed in ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2008 - American Association for Cancer Research | Cancer Research
Produção Nacional
Revisado por pares
Bruno Kaufmann Robbs, André L. S. Cruz, Miriam B. F. Werneck, Giuliana P. Mognol, João P. B. Viola,
Nuclear factor of activated T cells (NFAT) was first described as an activation and differentiation transcription factor in lymphocytes. Several in vitro studies suggest that NFAT family members are redundant proteins. However, analysis of mice deficient for NFAT proteins suggested different roles for the NFAT family of transcription factors in the regulation of cell proliferation and apoptosis. NFAT may also regulate several cell cycle and survival factors influencing tumor growth and survival. ...
Tópico(s): Cancer-related gene regulation
2008 - Taylor & Francis | Molecular and Cellular Biology
Produção Nacional
Revisado por pares
Bruna de Paula Fonseca e Fonseca, Priscilla C. Olsen, Luciana P. Coelho, Tatiana Paula Teixeira Ferreira, Heitor Siffert Pereira de Souza, Marco A. Martins, João P. B. Viola,
Allergic asthma is a chronic inflammatory disease of the lung whose incidence and morbidity continues to rise in developed nations. Despite being a hallmark of asthma, the molecular mechanisms that determine airway hyperresponsiveness (AHR) are not completely established. Transcription factors of the NFAT family are involved in the regulation of several asthma-related genes. It has been shown that the absence of NFAT1 leads to an increased pleural eosinophilic allergic response accompanied by an ...
Tópico(s): Cardiovascular, Neuropeptides, and Oxidative Stress Research
2008 - American Thoracic Society | American Journal of Respiratory Cell and Molecular Biology
Produção Nacional
Revisado por pares
Patrı́cia T. Bozza, João P. B. Viola,
Accumulation of lipid droplets (also known as lipid bodies or adiposomes) within leukocytes, epithelial cells, hepatocytes and other non-adipocytic cells is a frequently observed phenotype in infectious, neoplastic and other inflammatory conditions. Lipid droplet biogenesis is a regulated cellular process that culminates in the compartmentalization of lipids and of an array of enzymes, protein kinases and other proteins, suggesting that lipid droplets are inducible organelles with roles in cell signaling, ...
Tópico(s): Cancer, Lipids, and Metabolism
2010 - Elsevier BV | Prostaglandins Leukotrienes and Essential Fatty Acids
Produção Nacional
Revisado por pares
Alziana Moreno da Cunha Pedrosa, Ricardo Weinlich, Giuliana P. Mognol, Bruno Kaufmann Robbs, João P. B. Viola, Ana Čampa, Gustavo P. Amarante-Mendes,
Abstract Over the past 20 y, the hormone melatonin was found to be produced in extrapineal sites, including cells of the immune system. Despite the increasing data regarding the biological effects of melatonin on the regulation of the immune system, the effect of this molecule on T cell survival remains largely unknown. Activation-induced cell death plays a critical role in the maintenance of the homeostasis of the immune system by eliminating self-reactive or chronically stimulated T cells. Because ...
Tópico(s): Bioactive Compounds and Antitumor Agents
2010 - American Association of Immunologists | The Journal of Immunology
Produção Nacional
Revisado por pares
Priscilla C. Olsen, Tatiana Paula Teixeira Ferreira, Magda F. Serra, Francisco Alves Farias‐Filho, Bruna de Paula Fonseca e Fonseca, João P. B. Viola, Renato S.B. Cordeiro, P. M. R. Silva, Jorge Carlos Santos da Costa, Marco A. Martins,
Inhalation of the local anaesthetic lidocaine has been suggested to be beneficial for asthmatics, but airway anaesthesia is unpleasant and may exacerbate bronchoconstriction. Our previous study showed that inhalation of the lidocaine analogue JMF2-1 can elicit the anti-inflammatory properties of lidocaine without anaesthesia. This prompted further research on the mechanism of action and putative therapeutic application of JMF2-1.We tested the hypothesis that JMF2-1 would prevent allergen-induced ...
Tópico(s): Inhalation and Respiratory Drug Delivery
2010 - Wiley | Clinical & Experimental Allergy
Produção Nacional
Revisado por pares
Elizabeth S. Magalhães, Diego Mourão‐Sá, Adriana Vieira‐de‐Abreu, Rodrigo T. Figueiredo, Ana L. Pires, Francisco Alves Farias‐Filho, Bruna de Paula Fonseca e Fonseca, João P. B. Viola, Christine N. Metz, Marco A. Martins, Hugo C. Castro‐Faria‐Neto, Patrı́cia T. Bozza, Marcelo T. Bozza,
Macrophage migration inhibitory factor (MIF) is increased in asthmatic patients and plays a critical role in the pathogenesis of asthma. We show here that mice lacking MIF failed to develop airway hyper-responsiveness (AHR), tissue eosinophilia, and mucus metaplasia. Analysis of the bronchoalveolar fluids revealed a substantial reduction of IL-13, eotaxin and cysteinyl-leukotrienes. The lack of these cardinal features of asthma in MIF(-/-) mice occurs regardless of high concentrations of IL-4 in the ...
Tópico(s): Macrophage Migration Inhibitory Factor
2007 - Wiley | European Journal of Immunology
Produção Nacional
Revisado por pares
Lilian D. S. Carvalho, Leonardo K. Teixeira, Nina Carrossini, Anita T.N. Caldeira, K. Mark Ansel, Anjana Rao, João P. B. Viola,
AbstractThe NFAT (Nuclear Factor of Activated T cells) family of transcription factors plays a central role in the regulation of several genes related to the immune response. Recently, NFAT proteins have been implicated in the proliferation and differentiation of different cell types. Previous studies have shown that NFAT1-deficient mice display lymphocyte hyperproliferation, shortened cell cycle duration, and cyclin overexpression. Here, we demonstrate that cyclin A2 expression is upregulated in ...
Tópico(s): Signaling Pathways in Disease
2007 - Taylor & Francis | Cell Cycle
Produção Nacional
Revisado por pares
Miriam B. F. Werneck, Adriana Vieira‐de‐Abreu, Roger Chammas, João P. B. Viola,
Tópico(s): Signaling Pathways in Disease
2011 - Springer Science+Business Media | Cancer Immunology Immunotherapy
Produção Nacional
Revisado por pares
Flávia Raquel Gonçalves Carneiro, Renata Ramalho‐Oliveira, Giuliana P. Mognol, João P. B. Viola,
The nuclear factor of activated T cells (NFAT) family of transcription factors is expressed in a wide range of cell types and regulates genes involved in cell cycle, differentiation, and apoptosis. NFAT proteins share two well-conserved regions, the regulatory domain and the DNA binding domain. The N- and C-terminal ends are transactivation sites and show less sequence similarity, whereas their molecular functions remain poorly understood. Here, we identified a transcriptional repressor, interferon ...
Tópico(s): Immune Cell Function and Interaction
2011 - Taylor & Francis | Molecular and Cellular Biology
Produção Nacional
Revisado por pares
Tatiana Martins Tilli, Vanessa Ferreira Franco, Bruno Kaufmann Robbs, João Luiz Mendes Wanderley, Fabrício Ribeiro de Azevedo da Silva, Kivvi Duarte de Mello, João P. B. Viola, Georg F. Weber, Etel Rodrigues Pereira Gimba,
Ovarian carcinoma is one of the most aggressive gynecological diseases and generally diagnosed at advanced stages. Osteopontin (OPN) is one of the proteins overexpressed in ovarian cancer and is involved in tumorigenesis and metastasis. Alternative splicing of OPN leads to 3 isoforms, OPNa, OPNb, and OPNc. However, the expression pattern and the roles of each of these isoforms have not been previously characterized in ovarian cancer. Herein, we have evaluated the expression profiling of OPN isoforms ...
Tópico(s): Enzyme Structure and Function
2011 - American Association for Cancer Research | Molecular Cancer Research
Produção Nacional
Revisado por pares
Giuliana P. Mognol, Patrícia Savio de Araújo-Souza, Bruno Kaufmann Robbs, Leonardo K. Teixeira, João P. B. Viola,
A number of physiological processes in both normal and cancer cells are regulated by the proto-oncogene c-Myc. Among them, processes such as cell cycle regulation, apoptosis, angiogenesis and metastasis are also controlled by the nuclear factor of activated T cells (NFAT) family of transcription factors. It is already known that NFAT upregulates c-Myc expression by binding to an element located in the minimal c-Myc promoter. However, the importance of other NFAT sites in the context of the full promoter ...
Tópico(s): Cancer-related gene regulation
2012 - Taylor & Francis | Cell Cycle
Produção Nacional
Revisado por pares
Miriam B. F. Werneck, Eugênio D. Hottz, Patrı́cia T. Bozza, João P. B. Viola,
Chronic inflammation is a risk factor for the development of colon cancer, providing genotoxic insults, growth and pro-angiogenic factors that can promote tumorigenesis and tumor growth. Immunomodulatory agents can interfere with the inflammation that feeds cancer, but their impact on the transformed cell is poorly understood. The calcium/calcineurin signaling pathway, through activation of NFAT, is essential for effective immune responses, and its inhibitors cyclosporin A (CsA) and FK506 are used ...
Tópico(s): Immune Response and Inflammation
2012 - Taylor & Francis | Cell Cycle
Produção Nacional
Revisado por pares
Douglas V. Faget, Pedro I. Lucena, Bruno Kaufmann Robbs, João P. B. Viola,
The proteins belonging to the nuclear factor of activated T cells (NFAT) family of transcription factors are expressed in several cell types and regulate genes involved in differentiation, cell cycle and apoptosis. NFAT proteins share two conserved domains, the NFAT-homology region (NHR) and a DNA-binding domain (DBD). The N- and C-termini display two transactivation domains (TAD-N and TAD-C) that have low sequence similarity. Due to the high sequence conservation in the NHR and DBD, NFAT members have ...
Tópico(s): Aldose Reductase and Taurine
2012 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
João P. B. Viola, Lilian D. S. Carvalho, Bruna de Paula Fonseca e Fonseca, Leonardo K. Teixeira,
The nuclear factor of activated T cells (NFAT) family of transcription factors has been primarily identified in immune cells; however, these proteins have been recently found to be functionally active in several other non-immune cell types. NFAT proteins are activated upon different stimuli that lead to increased intracellular calcium levels. Regardless of their widely known cytokine gene expression properties, NFATs have been shown to regulate other genes related to cell cycle progression, cell ...
Tópico(s): Signaling Pathways in Disease
2005 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Produção Nacional
Revisado por pares
Leonardo K. Teixeira, Bruna de Paula Fonseca e Fonseca, Adriana Vieira‐de‐Abreu, Bianca A. Barboza, Bruno Kaufmann Robbs, Patrı́cia T. Bozza, João P. B. Viola,
Abstract CD8+ T lymphocytes are excellent sources of IFN-γ; however, the molecular mechanisms that dictate IFN-γ expression upon TCR stimulation in these cells are not completely understood. In this study, we evaluated the involvement of NFAT1 in the regulation of IFN-γ gene expression in murine CD8+ T cells and its relevance during Th differentiation. We show that CD8+, but not CD4+, T cells, represent the very first source of IFN-γ upon primary T cell activation, and also that the IFN-γ produced ...
Tópico(s): T-cell and B-cell Immunology
2005 - American Association of Immunologists | The Journal of Immunology
Produção Nacional
Revisado por pares
Leonardo K. Teixeira, Bruna PF Fonseca, Bianca A. Barboza, João P. B. Viola,
Allergic diseases have been closely related to Th2 immune responses, which are characterized by high levels of interleukin (IL) IL-4, IL-5, IL-9 and IL-13. These cytokines orchestrate the recruitment and activation of different effector cells, such as eosinophils and mast cells. These cells along with Th2 cytokines are key players on the development of chronic allergic inflammatory disorders, usually characterized by airway hyperresponsiveness, reversible airway obstruction, and airway inflammation. ...
Tópico(s): Urticaria and Related Conditions
2005 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Steven Xanthoudakis, João P. B. Viola, Karen T. Y. Shaw, Chun Huai Luo, J. Wallace, Patrı́cia T. Bozza, Daniel Luk, Tom Curran, Anjana Rao,
Transcription factors of the NFAT family are thought to play a major role in regulating the expression of cytokine genes and other inducible genes during the immune response. The role of NFAT1 was investigated by targeted disruption of the NFAT1 gene. Unexpectedly, cells from NFAT1 −/− mice showed increased primary responses to Leishmania major and mounted increased secondary responses to ovalbumin in vitro. In an in vivo model of allergic inflammation, the accumulation of eosinophils and levels of ...
Tópico(s): Signaling Pathways in Disease
1996 - American Association for the Advancement of Science | Science
Christine Loh, Karen T. Y. Shaw, Josephine A. Carew, João P. B. Viola, Chun Luo, Brian A. Perrino, Anjana Rao,
NFAT1 (previously termed NFATp) is a cytoplasmic transcription factor involved in the induction of cytokine genes. We have previously shown that the dephosphorylation of NFAT1, accompanied by its nuclear translocation and increased DNA binding activity, is regulated by calcium- and calcineurin-dependent mechanisms, as each of these hallmarks of NFAT1 activation is elicited by ionomycin and blocked by the immunosuppressive drugs cyclosporin A and FK506 (Shaw, K. T.-Y., Ho, A. M., Raghavan, A., Kim, ...
Tópico(s): Signaling Pathways in Disease
1996 - Elsevier BV | Journal of Biological Chemistry