Iola F. Duarte, Joana Marques, Ana Ladeirinha, Cláudia Rocha, Inês Lamego, Rita Calheiros, Tania Silva, M. Paula M. Marques, Joana Barbosa Melo, Isabel M. Carreira, Ana M. Gil,
The aim of this work was to investigate the effects of cell handling and storage on cell integrity and 1H high resolution magic angle spinning (HRMAS) NMR spectra. Three different cell types have been considered (lung tumoral, amniocytes, and MG-63 osteosarcoma cells) in order for sample-dependent effects to be identified. Cell integrity of fresh cells and cells frozen in cryopreservative solution was ∼70−80%, with the former showing higher membrane degradation, probably enzymatic, as indicated by ...
Tópico(s): Advanced MRI Techniques and Applications
2009 - American Chemical Society | Analytical Chemistry
Maria de Fátima Silva Lopes, C.I. Pereira, F. M. S. Rodrigues, Maria Paula Martins, M. C. Mimoso, T. C. Barros, Joana Marques, Rogério Tenreiro, Joana Almeida, Maria Teresa Barreto Crespo,
ABSTRACT Cheese produced from raw ewes’ milk and chouriço , a Portuguese dry fermented sausage, are still produced in a traditional way in certain regions of Portugal by relying on colonization by microbial populations associated with the raw materials, equipment, and local environments. For the purpose of describing the product origins and types of these fermented foods, metabolic phenotypes can be used as descriptors of the product as well as to determine the presence of compounds with organoleptic ...
Tópico(s): Meat and Animal Product Quality
1999 - American Society for Microbiology | Applied and Environmental Microbiology
Duarte Marques, António Mata, João Silveira, Joana Marques, João Amaral, Nuno Filipe Rito Parada Marques Guilherme,
Tópico(s): Temporomandibular Joint Disorders
2011 - Springer Nature | Clinical Oral Investigations
Hiddo J.L. Heerspink, Jai Radhakrishnan, Charles E. Alpers, Jonathan Barratt, Stewart Bieler, Ulysses Diva, Jula K. Inrig, Radko Komers, Alex Mercer, Irene L. Noronha, Michelle N. Rheault, William E. Rote, Brad H. Rovin, Howard Trachtman, Hernán Trimarchi, Muh Geot Wong, Vlado Perkovic, Eric Alarmartine, Jonathan Barratt, Dong‐Wan Chae, Lucia Del Vecchio, Jürgen Floege, Shang‐Jyh Hwang, Bojan Jelaković, Bart Maes, Robert Małecki, Marius Miglinas, Fernando Nolasco, M Praga, Kannaiyan S Rabindranath, Mai Rosenberg, Brad H. Rovin, Sydney C.W. Tang, Vladmir Tesar, Muh Geot Wong, Bhadran Bose, Muralikrishna Gangadharan, Stephen P. McDonald, Chen Au Peh, Sadia Jahan, Chii Yeap, Philip A. Clayton, Georgina Irish, Nikhil Thyagarajan, Peter Hollett, Rathika Krishnasamy, Robert J. Carroll, Shilpanjali Jesudason, Susan Crail, Toby Coates, Jane Waugh, Euan Noble, Kumaradevan Mahadevan, Victoria Campbell, Tania Salehi, Wai H. Lim, Neil Boudville, Aron Chakera, Doris Chan, Anoushka Krishnan, Yusuf Eqbal, Alastair Gillies, Eswari Vilayur, Thida Maung Myint, Nicholas A. Gray, Jane Waugh, Euan Noble, Melissa S Cheetham, Yusuf Eqbal, Peter Hollett, Rathika Krishnasamy, Kumaradevan Mahadevan, Victoria Campbell, Carol A. Pollock, Bruce A. Cooper, Amanda Mather, Sarah Roxburgh, Yvonne Shen, Stefanie Stangenberg, Amanda Siriwardana, Emma O’Lone, Susan Wan, Brendon L. Neuen, Jeffrey Ha, Dana Kim, Lauren Heath, Arunima Jain, Elaine Phua, Li Yan, M. W. Gallagher, Meg Jardine, Angus Ritchie, Mona Razavian, Celine Foote, Roger Wyndham, Shaundeep Sen, Zoltán Endre, Jonathan Erlich, Mangalee Fernando, Kenneth Yong, Grant Luxton, Sradha Kotwal, Simon D. Roger, Vidu Wijeratne, David Packham, Ian Fraser, Bert Vandewiele, Margo Laute, Wim Lemahieu, Sofie Jamar, Sara Ombelet, Gert Meeus, Marc Decupere, Olivier P. Schockaert, Peter Doubel, Liesbeth Viaene, Luc Radermacher, Catherine Masset, Martial Moonen, Éric Firre, Martina Milicevic, Xavier Warling, An Vanacker, Thomas Malfait, Ivan Durlen, Ivica Horvatić, Ana Šavuk, Lana Gellineo, Sandra Karanović, Živka Dika, Djuro Plavljanic, Ivana Mikačić, Dubravka Trajbar Kentric, Dunja Barisic, Marija Stanković, Karolina Majstorović Barać, Ivan Kruljac, Draško Pavlović, Martin Drinković, Ingrid Prkačin, Jerko Barbić, Zvonimir Sitaš, Dunja Vujčić, Ivan Rychlík, Anna Benesova, Klara Drinovska, Karolína Krátká, Dita Maixnerová, Madis Ilmoja, Kristin Unt, Kadri Lilienthal, Asta Auerbach, Liisi Leis, Julia Piel, Annika Adoberg, Külli Kõlvald, Kristi Veermae, Kadri Telling, Elviira Seppet, Jana Uhlinova, Philippe Zaoui, Pierre‐Louis Carron, Ingrid Masson, M. Dinic, Damien Thibaudin, C Broyet, Nicolas Maillard, Hesham Mohey, Christophe Mariat, Guillaume Claisse, É. Alamartine, Bertrand Dussol, Stéphane Burtey, Noemie Chiche-Jourde, Jean‐Emmanuel Serre, Guillaume Jeantet, Leïla Chénine, Anne Blanchard, Stéphane Roueff, Éric Thervet, David Fouassier, Alexandre Buffet, Marine Livrozet, Roxane Gaïsset, Alexandre Karras, Anne-Elisabeth Heng, Cyril Garrouste, Carole Philipponnet, C. Nicolò, Alba Atenza, Camille Lanaret, Clarisse Grèze, Valentin Mayet, Clément Dumond, Yahsou Delmas, Christian Combe, Claire Rigothier, Laure Burguet, Aurore Labat, Simon Mucha, V. de Précigout, Thomas Weinreich, Helmut Reichel, Diliana Draganova, Lothar Wolf, Bernd Hohenstein, Sven Heinrichs, Simone Kulka, Sebahat Şat, Lea Weiland, Thilo Krueger, Günter Wolf, Christiane Kettner, Mandy Schlosser, Johann Konstantin Herfurth, Annegret Koch, Martin Busch, Stephan Christian Werth, Martin Nitschke, Figen Cakiroglu, Franziska Sarnow, Lisa Schulz, Stefan M. Weiner, Nikolaus Wirtz, Eric Koester, Marcus J. Moeller, Eleni Stamellou, Silja K. Sanden, Hans Schmidt-Guertler, Wanja Bernhardt, Margret Patecki, Georg Schlieper, Kevin Schulte, Annette Girardet, Ulrich Kunzendorf, Lorraine Pui Yuen Kwan, Maggie Ming Yee Mok, Gary C.W. Chan, Mingyao Ma, Davina Ngoi Wah Lie, Anthony Ting Pong Chan, Cheuk‐Chun Szeto, Jack Kit‐Chung Ng, Siu Fai Cheung, Tak Tai Andrew Yue, Samuel Fung, Hon Tang, Ka Fai Yim, Wai Ping Law, Yick Hei Wong, Chi Kwan Darwin Lam, Sze Ho Sunny Wong, Carmelita Marcantoni, Roberta Aliotta, Francesca Deodato, Gemma Patella, Nicolino Comi, Caterina Vita, Nazareno Carullo, Davide Bolignano, Michela Musolino, Matias Trillini, Norberto Perico, Giuseppe Remuzzi, Erica Daina, Luigi Biancone, Loredana Colla, Manuel Burdese, C Cogno, Elena Boaglio, Isabella Abbasciano, Carlotta Federica Zizzi, Paolo Randone, Pietro Napodano, Anna Ricchiuto, Matthias Cassia, Simone Accarino, Mario Cozzolino, Rocco Baccaro, Stefano Costanzi, Federica Di Maio, Maria Arena, Federica Urciuolo, Sara Viganò, Andrea Cavalli, Monica Limardo, M Bordoli, Serena Ponti, Selena Longhi, Andrea Solazzo, Francesco Giaroni, Gabriele Donati, Massimo Torreggiani, Davide Catucci, Marco Colucci, Vittoria Esposito,
Sparsentan is a novel, non-immunosuppressive, single-molecule, dual endothelin and angiotensin receptor antagonist being examined in an ongoing phase 3 trial in adults with IgA nephropathy. We report the prespecified interim analysis of the primary proteinuria efficacy endpoint, and safety.PROTECT is an international, randomised, double-blind, active-controlled study, being conducted in 134 clinical practice sites in 18 countries. The study examines sparsentan versus irbesartan in adults (aged ≥18 ...
Elsevier BV
Patrícia Marques, Susana Fernandes, Filipa Carvalho, Alberto Barros, Mário Sousa, C. Joana Marques,
Summary Imprinting errors have been described in spermatozoa from infertile patients with oligozoospermia and azoospermia. However, little is known about methylation of imprinted genes in other spermatogenic cells from azoospermic patients. Therefore, we aimed to evaluate the methylation status of single CpGs located in the differentially methylated regions ( DMR s) of two imprinted genes, one paternally ( H19 ) and one maternally ( MEST ) methylated, in primary spermatocytes of azoospermic patients presenting ...
Tópico(s): Prenatal Screening and Diagnostics
2017 - Wiley | Andrology
Brad H. Rovin, Jonathan Barratt, Hiddo J.L. Heerspink, Charles E. Alpers, Stewart Bieler, Dong-Wan Chae, Ulysses Diva, Jürgen Floege, Loreto Gesualdo, Jula K. Inrig, Donald E. Kohan, Radko Komers, Laura Kooienga, Richard A. Lafayette, Bart Maes, Robert Małecki, Alex Mercer, Irene L. Noronha, Se Won Oh, Chen Au Peh, Manuel Praga, Priscila Preciado, Jai Radhakrishnan, Michelle N. Rheault, William E. Rote, Sydney C.W. Tang, Vladimı́r Tesař, Howard Trachtman, Hernán Trimarchi, James A. Tumlin, Muh Geot Wong, Vlado Perkovic, Isabella Abbasciano, Catarina Abrantes, Simone Accarino, Sharon G. Adler, Annika Adoberg, Rouzbeh Afsari, Syeda B. Ahmad, Jafar Ahmed, Wooin Ahn, Bamidele Ajayi, Dariusz Aksamit, Saif Al‐Chalabi, É. Alamartine, Bassam Alchi, Mohammad Ali, Roberta Aliotta, Salem Almaani, Catarina Almeida, Edgar Almeida, Francisco de la Prada Alvarez, Patrícia Marques‐Alves, Francesca Annese, Gerald B. Appel, Alberto Ortíz, Maria Arena, Marta Calvo Arevalo, Dariush Arfaania, Carlos Arias, Emma Calatayud Aristoy, Eglė Ašakienė, Sarah Ashley, Ali Assefi, Alba Atenza, Asta Auerbach, Hanna Augustyniak‐Bartosik, M Avella, Jonathan Ayling-Smith, Isabelle Ayoub, Christine Ayvazyan, Rocco Baccaro, Asha Bailey, Bruce Baker, Saravanan Balamuthusamy, José Ballarín, Rui Barata, Jerko Barbić, Dunja Barisic, Jonathan Barratt, José C. Barreto, Clara Barrios, Mirco Belingheri, Anna Benesova, Ana Ávila Bernabéu, Wanja Bernhardt, Shamik Bhadra, Luigi Biancone, Anne Blanchard, Elena Boaglio, Davide Bolignano, Andrew S. Bomback, Gustavo Andres Useche Bonilla, M Bordoli, Bhadran Bose, Neil Boudville, Donald Brandon, Donald Brandon, Karen Brown, C Broyet, Thomas Bucknall, Alexandre Buffet, Inga Arūnė Bumblytė, Manuel Burdese, Natalia Allende Burgos, Natalia Allende Burgos, Laure Burguet, Stéphane Burtey, Martin Busch, Figen Cakiroglu, Victoria Campbell, Pietro A. Canetta, Flavia Capaccio, Juan Carbonell, Filipa Cardoso, Clara García Carro, Robert J. Carroll, Pierre‐Louis Carron, Nazareno Carullo, Matthias Cassia, Dawn J. Caster, Belen Vizcaino Castillo, Davide Catucci, Andrea Cavalli, Dong-Wan Chae, Aron Chakera, Doris Chan, Gary C.W. Chan, Anthony Ting Pong Chan, Lai Wan Chan, Jae Hyun Chang, Ming-Shan Chang, Fiona A. Chapman, Chaim Charytan, Asghar Chaudhry, Melissa S Cheetham, Cheng‐Hsu Chen, Hsin-Yu Chen, Chien‐Liang Chen, Hung‐Yuan Chen, Hung‐Chun Chen, Leïla Chénine, Siu Fai Cheung, Chee Kay Cheung, Noemie Chiche-Jourde, Hsien‐Fu Chiu, Yen‐Ling Chiu, Yi‐Wen Chiu, Ian Chiu, Hoon Young Choi, Kang‐Ju Chou, Paramit Chowdhury, Pradip Chowdhury, Constantina Chrysochou, Chukwuma Chukwu, Ka Yeong Chun, Wookyung Chung, Dominik Cieniawski, Guillaume Claisse, Philip A. Clayton, Catalina Martín Cleary, Nisha Clement, Toby Coates, C Cogno, Loredana Colla, Michael Collins, Marco Colucci, Christian Combe, Nicolino Comi, Roderick Comunale, Gabriel Contreras, Bruce A. Cooper, Gaia Coppock, Ana Cortesão Costa, Stefano Costanzi, Mario Cozzolino, Susan Crail, Liliana Cunha, Elizabeth Curry, Tiane Dai, Erica Daina, Sam Daneshvari, Indranil Dasgupta, Louise DaSilva, Robert M. Davidson, Aine Maire De Bhailis, V. de Précigout, Marc Decupere, Yahsou Delmas, Francesca Deodato, Neeraj Dhaun, Ajay Dhaygude, Ranjit Dhelaria, Federica Di Maio, María Ángeles Goicoechea Diezhandino, Živka Dika, M. Dinic, Ian Dittmer, Mirela Dobre, Gabriel Doctor, Gabriele Donati, Peter Doubel, Nicole Douthit, Diliana Draganova, Yelena Drexler, Martin Drinković, Klara Drinovska, Caroline Dudreuilh, Clément Dumond, Ivan Durlen, Bertrand Dussol, Montserrat Díaz-Encarnación, Zoltán Endre, Jorge Enrique, Yusuf Eqbal, Jonathan Erlich, Vittoria Esposito, Ciro Esposito, Faisal Fakih, Hua‐Chang Fang, Ana Farinha, Aaron Fearday, Joana Felgueiras, Elzbieta Felicjanczuk, João Carlos Fernandes, Sara Fernandes, Adriana Fernandes, Jose Luño Fernandez, Víctor Fernández, Mangalee Fernando, Ana Figueiredo, Éric Firre, Jürgen Floege, Oliver Floßmann, Lauren Floyd, Nuno Moreira Fonseca, José Nuno Fonseca, Celine Foote, Suzanne Forbes, Ángel Blasco Forcén, Alessia Fornoni, David Fouassier, Ian Fraser, Samuel Fung, Mónica Furlano, Teresa Furtado, Rajdeep Gadh, Roxane Gaïsset, M. W. Gallagher, Joana Gameiro, Muralikrishna Gangadharan, Fabiola Alonso García, Olga Gracia Garcia, Gunjan Garg, Cyril Garrouste, Archana Gautam, Abdallah S. Geara, Colin Geddes, Lana Gellineo, Michael J. Germain, Loreto Gesualdo, Farid Ghalli, Francesco Giaroni, Harold Giles, Alastair Gillies, Ana Vilar Gimeno, Annette Girardet, Jeffrey Glaze, Tze Liang Goh, Mário Góis, Christopher Goldsmith, Elena Gomá-Garcés, Ana Marta Gomes, Luis Pedro Falcao Goncalves, Sara Gonçalves, Julita Gontarek-Kacprzak, Carlos González, Arun Gopu, Nayan Gowda, Nicholas A. Gray, Barbara Greco, Heather Green, Clarisse Grèze, Siân Griffin, Secundino Cigarrán, Úrsula Verdalles Guzmán, Jeffrey Ha, Amanda K. Hall, Patrick Hamilton, Seung Hyeok Han, Robert Haws, Ashik Hayat, Lauren Heath, Sven Heinrichs, Andrew Henderson, Amy Henderson,
Background Sparsentan, a novel, non-immunosuppressive, single-molecule, dual endothelin angiotensin receptor antagonist, significantly reduced proteinuria versus irbesartan, an angiotensin II receptor blocker, at 36 weeks (primary endpoint) in patients with immunoglobulin A nephropathy in the phase 3 PROTECT trial's previously reported interim analysis. Here, we report kidney function and outcomes over 110 weeks from the double-blind final analysis. Methods PROTECT, a double-blind, randomised, active- ...
Elsevier BV
P. Fanlo, Borja Gracia-Tello, Eva Fonseca Aizpuru, Jorge Álvarez Troncoso, Andrés González García, Sergio Prieto‐González, M. Freire, Ana Argibay, Lucio Pallarés, José Todolí, Mercedes Pérez, Segundo Buján-Rivas, Berta Ibáñez, Rubén Arnáez, Elisa Huarte, Julio Sánchez, Eva Zabalza, Ruth Garcia-Rey, Maria Belen Gonzalo, Laura Diez-Galán, Marisa de la Rica Escuín, Luis Martínez‐Lostao, Adela Marín Ballvé, María Luisa Taboada-Martínez, Rubén Pampín Sánchez, Cristina Helguera-Amézua, R. Fernández-Madera-Martínez, Estela García-Coya, Ana María Álvarez-Suarez, Ángel Ricardo Galarza Robles, Ana García‐Noblejas, Clara Soto, Elena Martínez, Francisco Arnalich, Coral Arévalo, Angélica López-Rodríguez, Pilar Cobeta, Fernando José Alcantarilla Hidalgo, Sergio Diz, Paula González, Nuria Bara, M. Fabregate, Judith Jiménez, Svetlana Zhilina Zhilina, Martina Pellicer-Ariño, Olga Núñez, Joan Ribot Grabalosa, Míriam Costafreda-Mas, Adrián Tomé-Pérez, Teresa Hospital-Vidal, Andrea Ladino-Vázquez, Alma Morancho-Sesé, Adelaido Salazar-Rustarazo, Cristina Gabara-Xancó, Arturo Gonzalez -Quintela, B. Sopeña, Rosario Alende -Sixto, Helena Esteban, Nuria Rodríguez‐Núñez, Ariadna Helena Andrade-Piña, Maria Ángeles Sanchidrian-Chapinal, Pablo Varela, Manuel Taboada, Brenda Maure-Noia, Ana López-Domínguez, Carmen Filloy-Mato, Beatriz Gimena-Reyes, Marisol Samartín‐Ucha, C. Vázquez-Triñanes, Julián Fernández-Martín, A Paradela Carreiro, Ana María Regueira‐Arcay, Eva Esteban-Marcos, Luisa Martin-Pena, Juan Antonio Fernández-Navarro, Noelia Abdilla-Bonias, Jaume Mestre-Torres, Joana Marques‐Soares, José Pardos‐Gea,
COVID-19 pneumonia is often associated with hyperinflammation. The efficacy and safety of anakinra in treating patients with severe COVID-19 pneumonia and hyperinflammation are still unclear.To assess the efficacy and safety of anakinra vs standard of care alone for patients with severe COVID-19 pneumonia and hyperinflammation.The Clinical Trial of the Use of Anakinra in Cytokine Storm Syndrome Secondary to COVID-19 (ANA-COVID-GEAS) was a multicenter, randomized, open-label, 2-group, phase 2/3 clinical ...
Tópico(s): Sepsis Diagnosis and Treatment
2023 - American Medical Association | JAMA Network Open
José M. Mascaró, Ignasi Rodríguez‐Pintó, Gabriela Poza, Anna Mensa‐Vilaró, Julián Fernández-Martín, Luis Caminal‐Montero, Gerard Espinosa, José Hernández‐Rodríguez, Marina Diaz, Joana Marques‐Soares, Raimón Sanmartí, Santos Castañeda, D. Colunga, Rubén Coto‐Hernández, P. Fanlo, Jose Ignacio Elejalde, Segundo Buján, Ignasi Figueras‐Nart, Francisco M. Marco, Mariano Andrés, Silvia Suárez, Andrés González García, X. Fustà‐Novell, Clara Garcia-Belando, A.L. Ortega Granados, María Teresa Fernández‐Figueras, Neus Quilis, Maria Orriols-Caba, Ricardo Gómez de la Torre, María C. Cid, Georgina Espígol‐Frigolé, Alba Alvarez-Abella, Eztizen Labrador, Marı́a Rozman, Mònica López‐Guerra, Paola Castillo, José R. Álamo-Moreno, Eva González‐Roca, Susana Plaza, Virginia Fabregat, Rocio Lara, Esther F. Vicente‐Rabaneda, Sonia Tejedor Vaquero, Giuliana Magri, Núria Bonet, Manuel Solís-Moruno, Andrea Cerutti, Òscar Fornas, Ferrán Casals, Jordi Yagüe, Juan I. Aróstegui,
The vacuoles, E1-enzyme, X linked, autoinflammatory and somatic (VEXAS) syndrome is an adult-onset autoinflammatory disease (AID) due to postzygotic UBA1 variants.To investigate the presence of VEXAS syndrome among patients with adult-onset undiagnosed AID. Additional studies evaluated the mosaicism distribution and the circulating cytokines.Gene analyses were performed by both Sanger and amplicon-based deep sequencing. Patients' data were collected from their medical charts. Cytokines were quantified ...
Tópico(s): Platelet Disorders and Treatments
2023 - BMJ | Annals of the Rheumatic Diseases
Mariana Cruz, Neusa Silva, Joana Marques, António Mata, F.S. Silva, João Caramês,
Background: The increased use of dental implants in oral rehabilitation has been followed by the development of new biomaterials as well as improvements in the performance of biomaterials already in use. This triggers the need for appropriate analytical approaches to assess the biological and, ultimately, clinical benefits of these approaches. Aims: To address the role of physical, chemical, mechanical, and biological characteristics in order to determine the critical parameters to improve biological ...
Tópico(s): Dental materials and restorations
2022 - Multidisciplinary Digital Publishing Institute | Biomimetics
Sofia S. Mendes, Joana Marques, Edit Mesterházy, Jan Straetener, Melina Arts, Teresa Pissarro, Jorgina Reginold, Anne Berscheid, Jan Bornikoel, Robert Maria Kluj, Christoph Mayer, Filipp Oesterhelt, Sofia Friães, Beatriz Royo, Tanja Schneider, Heike Brötz‐Oesterhelt, Carlos C. Romão, Lı́gia M. Saraiva,
Several metal-based carbon monoxide-releasing molecules (CORMs) are active CO donors with established antibacterial activity. Among them, CORM conjugates with azole antibiotics of type [Mn(CO)3(2,2'-bipyridyl)(azole)]+ display important synergies against several microbes. We carried out a structure-activity relationship study based upon the lead structure of [Mn(CO)3(Bpy)(Ctz)]+ by producing clotrimazole (Ctz) conjugates with varying metal and ligands. We concluded that the nature of the bidentate ligand ...
Tópico(s): CO2 Reduction Techniques and Catalysts
2022 - American Chemical Society | ACS Bio & Med Chem Au
Jaume Alijotas‐Reig, Enrique Esteve‐Valverde, Ariadna Anunciación-Llunell, Joana Marques‐Soares, José Pardos‐Gea, Francesc Miró‐Mur,
Antiphospholipid syndrome is an autoimmune disorder characterized by vascular thrombosis and/or pregnancy morbidity associated with persistent antiphospholipid antibody positivity. Cases fulfilling the Sydney criteria for obstetric morbidity with no previous thrombosis are known as obstetric antiphospholipid syndrome (OAPS). OAPS is the most identified cause of recurrent pregnancy loss and late-pregnancy morbidity related to placental injury. Cases with incomplete clinical or laboratory data are ...
Tópico(s): Blood Coagulation and Thrombosis Mechanisms
2022 - Multidisciplinary Digital Publishing Institute | Journal of Clinical Medicine
José Pardos‐Gea, Joana Marques‐Soares, Segundo Buján, José Ordi-Ros, Jaume Alijotas‐Reig,
To investigate the impact of thrombocytopenia on survival in patients with APS.Thrombocytopenia and other predictors of outcome were retrospectively evaluated in an aPL-positive and APS cohort with 38-year follow-up (1980-2018). Thrombocytopenia was defined as <150 × 109 platelets/l. Hazard ratios (HR) of mortality were calculated using Cox-regression models.Among 114 patients, 64% had primary APS, 25% secondary APS and 10% asymptomatic aPL. Mean follow-up was 19 (range 5-38) years. ANA [hazard ratio (HR) ...
Tópico(s): Renal Diseases and Glomerulopathies
2021 - Oxford University Press | Lara D. Veeken
Liina Nagirnaja, Nina Mørup, John Nielsen, Rytis Stakaitis, Ieva Golubickaitė, Manon S. Oud, Sofia B. Winge, Filipa Carvalho, Kenneth I. Aston, Francesca Khani, Godfried W. van der Heijden, C. Joana Marques, Niels E. Skakkebæk, Ewa Rajpert‐De Meyts, Peter N. Schlegel, Niklas Rye Jørgensen, Joris A. Veltman, Alexandra M. Lopes, Donald F. Conrad, Kristian Almstrup,
P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are short (21 to 35 nucleotides in length) and noncoding and are found almost exclusively in germ cells, where they regulate aberrant expression of transposable elements and postmeiotic gene expression. Critical to the processing of piRNAs is the protein poly(A)-specific RNase-like domain containing 1 (PNLDC1), which trims their 3' ends and, when disrupted in mice, causes azoospermia and male infertility.We performed exome sequencing on ...
Tópico(s): CRISPR and Genetic Engineering
2021 - Massachusetts Medical Society | New England Journal of Medicine
Daniela Ionescu, Alejandro Peñín‐Franch, Anna Mensa‐Vilaró, Paola Castillo, Laura Hurtado‐Navarro, Cristina Molina‐López, Silvia Romero‐Chala, Susana Plaza, Virginia Fabregat, Segundo Buján, Joana Marques, Ferrán Casals, Jordi Yagüe, Baldomero Oliva, Luis Fernández Pereira, Pablo Pelegrı́n, Juan I. Aróstegui,
Autoinflammatory diseases are inherited disorders of innate immunity that usually start during childhood. However, several recent reports have described an increasing number of patients with autoinflammatory disease starting in adulthood. This study was undertaken to investigate the underlying cause of a case of late-onset uncharacterized autoinflammatory disease.Genetics studies were performed using Sanger sequencing and next-generation sequencing (NGS) methods. In silico, in vitro, and ex vivo ...
Tópico(s): Eosinophilic Esophagitis
2021 - Wiley | Arthritis & Rheumatology
Cláudia Antunes, Jorge Diogo Da Silva, Sónia Guerra‐Gomes, Nuno Dinis Alves, F. Ferreira, Eduardo Loureiro‐Campos, Miguel R. Branco, Nuno Sousa, Wolf Reik, Luísa Pinto, C. Joana Marques,
Tópico(s): Birth, Development, and Health
2020 - Springer Nature | Molecular Psychiatry
Produção Nacional
Revisado por pares
Mariana Cruz, Joana Marques, Gabriella M. Peñarrieta‐Juanito, M.M. Costa, Júlio C. M. Souza, Ricardo S. Magini, G. Miranda, F.S. Silva, João Caramês, António Mata,
The aim of this study was to characterize the mechanical properties of a bioactive-modified polyetheretherketone (PEEK) manufacturing approach for dental implants and to compare the in vitro biological behavior with titanium alloy (Ti6Al4V) as the reference. PEEK, PEEK with 5% hydroxyapatite (HA), PEEK with 5% beta-tricalcium phosphate (βTCP), and Ti6Al4V discs were produced using hot pressing technology to create a functionally graded material (FGM). Surface roughness values (Ra, Rz), water contact angle, ...
Tópico(s): Dental Implant Techniques and Outcomes
2020 - Allen Press | Journal of Oral Implantology
Daniela Duarte, Beatriz Castro, Joana Leonor Pereira, Joana Marques, Ana Luísa Costa, Ana M. Gil,
Maintaining a salivary metabolic profile upon sample collection and preparation is determinant in metabolomics. Nuclear magnetic resonance (NMR) spectroscopy was used to identify metabolite changes during short-term storage, at room temperature (RT)/4 °C/−20 °C, and after sample preparation, at RT/4 °C (mimicking typical clinical/laboratory settings). Interestingly, significant metabolic inter-individual and inter-day variability were noted, probably determining sample stability to some extent. After ...
Tópico(s): Traditional Chinese Medicine Studies
2020 - Multidisciplinary Digital Publishing Institute | Metabolites
Joana Marques, Adrien Marronnier, Frédéric Crampon, Aude Lagier, Jean‐Paul Marie,
Unilateral vocal fold paralysis (UVFP) often leads to significant morbidity that may include dysphonia, swallowing problems and aspiration. The best timing for medialization procedures is still controversial. Published data suggest that early intracordal injection positively affects long-term outcomes.To critically review current literature in order to determine if early treatment of acute UVFP influences clinical outcomes of the patients.Nonsystematic literature review.A literature review was performed, ...
Tópico(s): Dysphagia Assessment and Management
2020 - Elsevier BV | Journal of Voice
Alexandra R. Fernandes, Inês Mendonça-Martins, Marino F. A. Santos, Luís R. Raposo, Rita Mendes, Joana Marques, Carlos C. Romão, Maria João Romão, Teresa Santos‐Silva, Pedro V. Baptista,
CO-releasing molecules (CORMs) have been widely studied for their anti-inflammatory, antiapoptotic, and antiproliferative effects. CORM-3 is a water-soluble Ru-based metal carbonyl complex, which metallates serum proteins and readily releases CO in biological media. In this work, we evaluated the anti-inflammatory and wound-healing effects of gold nanoparticles–CORM-3 conjugates, AuNPs@PEG@BSA·Ru(CO)x, exploring its use as an efficient CO carrier. Our results suggest that the nanoformulation was ...
Tópico(s): Thermal Regulation in Medicine
2020 - American Chemical Society | ACS Biomaterials Science & Engineering
Martin Darino, Khong‐Sam Chia, Joana Marques, Dávid Aleksza, Luz Mayela Soto-Jiménez, Indira Saado, Simon Uhse, Michael Borg, Ruben Betz, János Bindics, Krzysztof Zienkiewicz, Ivo Feußner, Yohann Petit, Armin Djamei,
Summary Ustilago maydis is the causal agent of maize smut disease. During the colonization process, the fungus secretes effector proteins that suppress immune responses and redirect the host metabolism in favor of the pathogen. As effectors play a critical role during plant colonization, their identification and functional characterization are essential to understanding biotrophy and disease. Using biochemical, molecular, and transcriptomic techniques, we performed a functional characterization ...
Tópico(s): Insect-Plant Interactions and Control
2020 - Wiley | New Phytologist
Berta Carvalho, C. Joana Marques, Rita Santos Silva, Manuel Fontoura, Davide Carvalho, Filipa Carvalho,
Congenital Adrenal Hyperplasia is a group of genetic autosomal recessive disorders that affects adrenal steroidogenesis in the adrenal cortex. One of the most common defects associated with Congenital Adrenal Hyperplasia is the deficiency of 21-hydroxylase enzyme, responsible for the conversion of 17-hydroxyprogesterone to 11-deoxycortisol and progesterone to deoxycorticosterone. The impairment of cortisol and aldosterone production is directly related to the clinical form of the disease that ranges ...
Tópico(s): Hormonal and reproductive studies
2020 - Thieme Medical Publishers (Germany) | Experimental and Clinical Endocrinology & Diabetes
Sara Vasconcelos, Carla Ramalho, C. Joana Marques, Sofia Dória,
Epigenetic mechanisms such as genomic imprinting have a fundamental role in embryo and fetal development. Hence, we here studied expression levels of epigenetic modifiers and imprinted genes in cases of ididopathic spontaneous abortion (SA). Thirty-five placental samples and 35 matched fetal tissues from second trimester SA were analysed; including 16 controls (placental and fetal infections as the known cause of spontaneous abortion) and 19 idiopathic SA cases. Transcript levels of epigenetic regulators ...
Tópico(s): Epigenetics and DNA Methylation
2019 - Landes Bioscience | Epigenetics
Gil Coutinho, Joana Marques, Manuel M. Cardoso Leal, Jorge Spratley, Margarida Sá Fernandes, Margarida Santos,
Inverted papillomas represent one of the most common benign neoplasic lesions located in the sinonasal tract. Owing to the local erosive behavior, tendency to recur and the potential for malignant transformation, surgical management of inverted papillomas is often challenging.This study aimed to analyze the surgical outcomes of patients with inverted papillomas, according to the Krouse staging and the different surgical approaches.Retrospective study of patients diagnosed with sinonasal inverted ...
Tópico(s): Meningioma and schwannoma management
2019 - Elsevier BV | Brazilian Journal of Otorhinolaryngology
Joana Marques, Artur M. S. Silva, M. Paula M. Marques, Susana S. Braga,
This study describes a simple, two-step method for obtaining ruthenium(II) trithiacyclononane ([9]aneS3) flavonate complexes in yields ranging from 23% to 34%. With 7,3′,4′-trihydroxyflavone (thflv), a neutral complex, [Ru(II)([9]aneS3)(DMSO)(thflv)] (1), is formed by coordination at the catechol group. With chrysin (chrys) and tectochrysin (tchrys), Ru(II) binds to the chromenone fragment to form cationic complexes that are isolated as [Ru(II)([9]aneS3)(chrys)(DMSO)]Cl (2) and [Ru(II)([9]aneS3)(DMSO)(tchrys)] ...
Tópico(s): Bioactive Compounds in Plants
2019 - Elsevier BV | Inorganica Chimica Acta
Cláudia Antunes, Nuno Sousa, Luísa Pinto, C. Joana Marques,
The dynamic nature of epigenetic DNA modifications is crucial for regulating gene expression in an experience-dependent manner and, thus, a potential mediator of neuronal plasticity and behavior. The discovery of the involvement of 5-hydroxymethylcytosine (5hmC) and Ten Eleven Translocation (TET) family of enzymes in the demethylation pathway uncovered a potential link between neuronal TET protein function and cognitive processes. In this review, we provide an overview on how profile of 5hmC and TET ...
Tópico(s): Adolescent and Pediatric Healthcare
2019 - Elsevier BV | Neuroscience & Biobehavioral Reviews
Mafalda Santiago, Cláudia Antunes, Marta Guedes, Michelina Iacovino, Michael Kyba, Wolf Reik, Nuno Sousa, Luísa Pinto, Miguel R. Branco, C. Joana Marques,
Abstract TET enzymes oxidize 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), a process thought to be intermediary in an active DNA demethylation mechanism. Notably, 5hmC is highly abundant in the brain and in neuronal cells. Here, we interrogated the function of Tet3 in neural precursor cells (NPCs), using a stable and inducible knockdown system and an in vitro neural differentiation protocol. We show that Tet3 is upregulated during neural differentiation, whereas Tet1 is downregulated. ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2019 - Springer Nature | Cellular and Molecular Life Sciences
Daniela Barros‐Silva, C. Joana Marques, Rui Henrique, Cármen Jerónimo,
DNA methylation is an epigenetic modification that plays a pivotal role in regulating gene expression and, consequently, influences a wide variety of biological processes and diseases. The advances in next-generation sequencing technologies allow for genome-wide profiling of methyl marks both at a single-nucleotide and at a single-cell resolution. These profiling approaches vary in many aspects, such as DNA input, resolution, coverage, and bioinformatics analysis. Thus, the selection of the most ...
Tópico(s): Cancer-related gene regulation
2018 - Multidisciplinary Digital Publishing Institute | Genes
Elisabet Martí Coma-Cros, Arnau Biosca, Joana Marques, Laura López Carol, Patricia Urbán, Diana Berenguer, Cristina Riera, Michael J. Delves, Robert E. Sinden, Juan José Valle‐Delgado, Lefteris Spanos, Inga Sidén‐Kiamos, Paula Cordero-Pérez, Krijn P. Paaijmans, Matthias Rottmann, Amedea Manfredi, Paolo Ferruti, Elisabetta Ranucci, Xavier Fernández‐Busquets,
Current strategies for the mass administration of antimalarial drugs demand oral formulations to target the asexual Plasmodium stages in the peripheral bloodstream, whereas recommendations for future interventions stress the importance of also targeting the transmission stages of the parasite as it passes between humans and mosquitoes. Orally administered polyamidoamine (PAA) nanoparticles conjugated to chloroquine reached the blood circulation and cured Plasmodium yoelii-infected mice, slightly ...
Tópico(s): HIV Research and Treatment
2018 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Produção Nacional
Revisado por pares
Joana Marques, João C. R. Cardoso, Rute C. Félix, Rosa Amélia Gonçalves Santana, Maria das Graças Vale Barbosa Guerra, Deborah M. Power, Henrique Silveira,
Abstract Mosquito breeding depends on the supply of fresh vertebrate blood, a major bottleneck for large-scale production of Anopheles spp. Feeding alternatives to fresh blood are thus a priority for research, outdoor large-cage trials and control interventions. Several artificial meal compositions were tested and Anopheles oogenesis, egg laying and development into the next generation of adult mosquitoes were followed. We identified blood-substitute-diets that supported ovarian development, egg ...
Tópico(s): Insect Utilization and Effects
2018 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Gabriella M. Peñarrieta‐Juanito, M.M. Costa, Mariana Cruz, G. Miranda, Bruno Henriques, Joana Marques, Ricardo de Souza Magini, António Mata, João Caramês, F.S. Silva, Júlio C. M. Souza,
Abstract The aim of this study was to analyze the osteogenic cell behavior on the surface of novel functionally graded titanium‐based composites containing bioactive ceramics. Titanium grade V discs (8 × 3 mm) embedding gradual content of hydroxyapatite (TiAlV‐HA) or beta‐tricalcium phosphate (TiAlV‐βTCP) were produced by hot‐pressing technique. Titanium‐ceramic composite discs and Ti grade V (control group) were placed in contact with human osteoblast culture assays. The morphology and adhesion of osteoblasts ...
Tópico(s): Titanium Alloys Microstructure and Properties
2018 - Wiley | Journal of Biomedical Materials Research Part A