Gráinne Gilmore, Andrew Robson, Giles Smith, Lucy Alexander, Simon Barnes Chief Sports Writer, Tony Doggart, Ivan Croxford Head of BT Tradespace, Sam Rumbelow, Alexandra Frean, Zahid Hussain, Martin Birchall, Peter Collett, Frances Gibb Legal Editor, Roger Braban, Gary Duncan, Robert Lindsay, James Rossiter Property Correspondent, David Rose, Philip Howard, Katie Scott, Hilary Finch, Camilla Cavendish, Imelda Redmond Chief Executive, Christine Seib, David Chater, James Bone, James Ducker, Penny Wark, Richard Hobson One-Day Cricket Correspondent, Dom Alberic Stacpoole, Elizabeth Judge, Nick Royle, Will Pavia, Sam Marlowe, James Hider, Allan Lutton, Jeremy Whittle, Kevin Eason, Irwin Stelzer, Joe Joseph, Stephen Waley-Cohen, Lynda Purser, Director, Institute of Business Consulting, Pat Gibson, Jane MacArtney, Valerie Elliott, Deborah Haynes, Mariella K. Thornton Former CSA Team Leader, Dominic Walsh, Ruth Gledhill, Dan Sabbagh Media Editor, Rory Watson, Gary Jacob, Ken Russell, Melanie Reid, Ruth Gledhill Religion Correspondent, Dalya Alberge Arts Correspondent, Dan Sabbagh, Nigel Newton, Richard Ford Home Correspondent, Elizabeth Colman, Jenny MacArthur, Anatole Kaletsky, Kevin Stagg, Valerie Elliott Consumer Editor, Rhys Blakely, Theresa Musgrove, Charles Bremner, Ron Lewis, Adam Sherwin Media Correspondent, Lisa Armstrong Fashion Editor, Greg Hurst, William Shawcross, David Charter, Edward Gorman, Hugo Rifkind, Derwent May, Tim Teeman, Russell Kempson, Suzy Jagger, Gary Duncan Economics Editor, Pete Dean, David Lister, Sarah Vine, Mark Henderson Science Editor, John Westerby, Tom Baldwin, Jill Sherman Whitehall Editor, Ann Treneman, David Robertson Business Correspondent, Paul McKenna, John Hopkins Golf Correspondent, Rob Wright, Geoffrey Dean, Alice Olins, Edward Gorman Motor Racing Correspondent, Neil Harman Tennis Correspondent, Raymond Keene, Lewis Smith, Harriet Lane, Jo Causon, Director, Sarah Birke, Patrick Hosking Business Commentry, David Sigall, Christine Buckley Industrial Editor, Francis Elliott Chief Political Correspondent, Alan Forward, Steve Coomber, Jeremy Page, Dr John MacDONALD, Chris Campling, Patrick Hosking, Helen Nugent, Richard Owen, Sandra Parsons, Christopher Martin-Jenkins Chief Cricket Correspondent, Janet Howd, Alan Copage, Nick Hasell, Dalya Alberge, David Hands Rugby Correspondent, Owen Slot Chief Sports Reporter, Rosemary Bennett Social Affairs Correspondent, Julian Muscat, Paul Simons, Kevin Maher, Michael Herman, Jane Gibbs, John Foster, Jonathan Clayton, Robert Crampton, Steve Hawkes, Jane Shilling, Thomas Catán, Emily Ford, Dominic Maxwell, Matthew Parris, Mike Rosewell, George Caulkin, Sonia Verma, Sophie Yu, Neil Fisher, Joshua Rowe, Peter Riddell, Andrew Ellson, Tony Dawe, Dr Jeremy Prout, Carly Chynoweth, Tom Hennigan, Philip Webster, John Naish, Mark Henderson, Martin Waller, Lewis Smith Environment Reporter, Alan Johnston, Patrick Foster, Carl Mortished International Business Editor, Nigel Hawkes Health Editor, Sarah Butler, Lee Bowman, Benedict Nightingale, Michael Bright, Angus Batey, Dr Thomas Stuttaford, Stephen Dalton, Nicola Woolcock, Richard Morrison, Tony Halpin, Alice Miles, James Christopher, Michael Evans, Alexandra Blair Education Correspondent, Olav Bjortomt, Miles Costello, Richard Ford, Ross Anderson, Sheila Collins, Joe Bolger, Elizabeth Judge Telecoms Correspondent, Nicola Thompson,
... of 50, but in Die Hard 4.0 John McClane is a hero for our times, says James ...
2007 - Gale Group | TDA
Trudi L. Bannam, Xuxia Yan, Paul F. Harrison, Torsten Seemann, Anthony L. Keyburn, Christopher J. Stubenrauch, Lakmini Hasanka Weeramantri, Jackie K. Cheung, Bruce A. McClane, John D. Boyce, Robert J. Moore, Julian I. Rood,
The pathogenesis of avian necrotic enteritis involves NetB, a pore-forming toxin produced by virulent avian isolates of Clostridium perfringens type A. To determine the location and mobility of the netB structural gene, we examined a derivative of the tetracycline-resistant necrotic enteritis strain EHE-NE18, in which netB was insertionally inactivated by the chloramphenicol and thiamphenicol resistance gene catP. Both tetracycline and thiamphenicol resistance could be transferred either together ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2011 - American Society for Microbiology | mBio
Steven J. McClane, John L. Rombeau,
Crohn’s disease (CD) and ulcerative colitis (UC) are the two major forms of inflammatory bowel disease (IBD) that can affect the rectum and result in the development of perianal disease characterized mainly by fistulas, specifically in CD patients. Other anorectal disorders comprise the presence of tags, hemorrhoids, perianal abscesses, and fissures. Multidisciplinary collaboration between gastroenterologists, colorectal surgeons, and other specialists is essential for the success of medical or surgical ...
Tópico(s): Diverticular Disease and Complications
2001 - Elsevier BV | Surgical Clinics of North America
Steven J. McClane, John L. Rombeau,
The etiology and pathogenesis of inflammatory bowel disease (IBD) remains an area under intense investigation. Cytokine secretion, which is important in the regulation of normal gastrointestinal immune responses, appears to be dysregulated in IBD. In Crohn's disease, there appears to be an excessive TH1 T‐cell response to an antigenic stimulus, leading to increased levels of proinflammatory cytokines, such as interferon‐γ (IFN‐γ), interleukin (IL)‐12, IL‐1, IL‐6, and tumor necrosis factor‐α (TNFa). ...
Tópico(s): Celiac Disease Research and Management
1999 - Wiley | Journal of Parenteral and Enteral Nutrition
G. Michael Vincent, Jolene Fox, G. Charlton, Joachim Hill, Robert W. McClane, John D. Spikes,
Abstract Application of 630 nm light in the presence of blood is often necessary during photodynamic therapy, particularly for proposed intravascular applications. The effect of blood on transmission of 630 nm light was studied using a three dimensional irradiation model and an integrating sphere for measuring light transmitted in any direction through blood layers of different hematocrit (25 to 75) and thickness (.15 to .98 mm). There was an inverse relationship between transmission and hematocrit ...
Tópico(s): Ocular and Laser Science Research
1991 - Wiley | Lasers in Surgery and Medicine
Nicholas Garcia, Sangita Kapur, JIM McCLANE, John Mihran Davis,
Hypothesis: This study was undertaken to assess the use of prophylactic antibiotics (PA) in laparoscopic and open cholecystectomy with respect to the postoperative infection rate and the established criteria for PA use. Methods: A total of 341 consecutive cases for 1994 were retrospectively analyzed. PA use was deemed appropriate if the patient: was over 70 years of age, had common duct stones, jaundice, or acute cholecystitis. Results: The infection rate of patients having an open cholecystectomy ...
Tópico(s): Appendicitis Diagnosis and Management
1997 - Mary Ann Liebert, Inc. | Journal of Laparoendoscopic & Advanced Surgical Techniques
Men’s upper body underwear and the depiction of grime, dirt and blood on costumes have a long tradition in Hollywood films. This article explores the 34 undershirts worn by Bruce Willis and his stuntman in the 1988 action film Die Hard from the points of view of the maker, designer, actor, curator and spectator. The image of McClane and the undershirt became iconic in their depiction of a white, working-class, heroic masculinity. One of the many undershirts used in the film was donated to the Smithsonian’ ...
Tópico(s): Cinema and Media Studies
2019 - Intellect | Studies in Costume & Performance
Merril T. Dayton, D. L. Decker, Robert W. McClane, John A. Dixon,
Tópico(s): Dental Anxiety and Anesthesia Techniques
1988 - Elsevier BV | Journal of Surgical Research
Thomas A. Hamilton, Ronald P. DeMatteo, Steven J. McClane, Charlotte Burke, John L. Rombeau, Steven E. Raper,
Purpose: The authors sought to assess the feasibility of in vivo gene transfer to the small intestine using recombinant adenovirus in neonatal and adult mice. Methods: H5.010CMVlacZ is a replication-defective, E1-deleted human type 5 adenovirus, which contains the lacZ gene under the control of a cytomegalovirus promoter and enhancer. The lacZ gene was used as a marker because its gene product, β-galactosidase, is readily detected by X-gal histochemistry. Sixty neonatal (3 to 5 days old) and 45 adult ( ...
Tópico(s): Infant Nutrition and Health
1997 - Elsevier BV | Journal of Pediatric Surgery
Elizabeth Shane, Kenneth A. McClane, Marcelo R. Olarte, John P. Bilezikian,
We studied a patient with hypocalcemia and increased serum activity of sarcoplasmic enzymes. Idiopathic hypoparathyroidism was established by history, low serum parathyroid hormone content, and marked responsiveness of urinary cyclic AMP and phosphate to parathyroid hormone. Muscle-associated isoenzymes of creatine phosphokinase and lactic dehydrogenase were increased, but there was no concomitant muscle weakness. Muscle biopsy was normal by morphologic and histochemical examination. The patient ...
Tópico(s): Genetic Syndromes and Imprinting
1980 - Lippincott Williams & Wilkins | Neurology
Jihong Li, Daniel R. Evans, John C. Freedman, Bruce A. McClane,
ABSTRACT Clostridium perfringens can produce up to three different sialidases, including NanI, its major exosialidase. The current study first showed that human intestinal strains of C. perfringens can grow by utilizing either glucose or sialic acids, such as N -acetylneuraminic acid (Neu5Ac), which are the end products of sialidase activity. For the human enteropathogenic strain F4969, it was then determined that culture supernatant sialidase activity and expression of exosialidase genes, particularly ...
Tópico(s): Bacterial Genetics and Biotechnology
2017 - American Society for Microbiology | Infection and Immunity
John C. Freedman, Bruce A. McClane, Francisco A. Uzal,
Epsilon toxin (ETX), produced by Clostridium perfringens types B and D, is responsible for diseases that occur mostly in ruminants. ETX is produced in the form of an inactive prototoxin that becomes proteolytically-activated by several proteases. A recent ex vivo study using caprine intestinal contents demonstrated that ETX prototoxin is processed in a step-wise fashion into a stable, active ∼27 kDa band on SDS-PAGE. When characterized further by mass spectrometry, the stable ∼27 kDa band was shown ...
Tópico(s): Gastrointestinal motility and disorders
2016 - Elsevier BV | Anaerobe
Jihong Li, John C. Freedman, Bruce A. McClane,
ABSTRACT Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat.1002429 ) that BMC202, a nanI null mutant of type D strain CN3718, produces less ETX than wild-type CN3718 does. The current study proved that the lower ETX production by strain BMC202 is ...
Tópico(s): Streptococcal Infections and Treatments
2015 - American Society for Microbiology | Journal of Bacteriology
Richard C. Straight, Robert E. Benner, Robert W. McClane, P. M. N. Y. H. Go, Gilwon Yoon, John A. Dixon,
Abstract— —Laser and fluorescence light distributions with applications for photodynamic therapy were measured in mouse tumors using a non‐invasive electronic optical imaging system. The system consists of a liquid‐nitrogen‐cooled, charge‐coupled‐device (CCD) array camera under computer control with 576 × 384 detection elements having dimensions of 23 μm × 23 μm. The available dynamic range of the array is approx. 10 3 , and the effective wavelength range is 400–1000 nm. An interstitially placed cylindrical ...
Tópico(s): Advanced Fluorescence Microscopy Techniques
1991 - Wiley | Photochemistry and Photobiology
Robert E. Benner, Robert W. McClane, John A. Dixon, Richard C. Straight,
An electronic optical imaging system consisting of a computer-controlled CCD array with 576 x 384 detection elements and 14 bits of digitization is presented. The system is used to obtain internal and external light intensity distributions for diffusing optical fiber tips used with photodynamic therapy and laser angioplasty. Significant intensity distribution variations were observed between fiber tips from 5 different sources. The effect of launch numerical aperture on the measured light distributions ...
Tópico(s): Optical Coherence Tomography Applications
1990 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Erich W. Bruhn, P. M. N. Y. H. Go, Robert W. McClane, John G. Hunter, Richard C. Straight,
Fiber tip breakage during urinary and biliary laser lithotripsy has been recognized to occur with several laser types. This phenomenon has also been seen with Q-switched Nd:YAG laser lithotripsy. Our aim was to determine the biological consequences of this event in the canine ureter and bile duct. In an excised tissue preparation, urinary and biliary stones were impacted in a canine ureter and common bile duct. Three and four hundred micron quartz laser fibers were placed in direct contact with ...
Tópico(s): Laser Applications in Dentistry and Medicine
1990 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Tópico(s): Contemporary Literature and Criticism
2017 - | The International Journal of Literary Humanities
Die Arbeit thematisiert die Hegemonie neoliberaler Mannlichkeiten anhand zweier Reprasentationen in zeitgenossischen Massenmedien. Der (inzwischen ehemalige) Deutsche Verteidigungsminister wird mit dem Protagonist eines popularen Kinofilmes kontrastiert um zu zeigen, wie vermeintlich gegensatzliche Charaktere neoliberale Ideologie reproduzieren. Hegemoniale Mannlichkeit wird dabei als abstraktes Einzelnes konzipiert, das konvergente Mannlichkeiten hervorbringt, aus deren Schnittmenge es entsteht.
Tópico(s): Sociology and Education Studies
2012 - Innsbruck University Press | Österreichische Zeitschrift für Politikwissenschaft/ÖZP. Österreichische Zeitschrift für Politikwissenschaft
Eva Wieckowski, John F. Kokai–Kun, Bruce A. McClane,
ABSTRACT After binding, Clostridium perfringens enterotoxin (CPE) initially localizes in a small (∼90-kDa) complex in plasma membranes. This event is followed by formation of a second membrane complex, referred to as large (160-kDa) complex. Contrary to a previous hypothesis proposing that CPE inserts into intestinal brush border membranes (BBMs) when this toxin is localized in the small complex, this study shows that BBMs do not offer CPE localized in the small complex protection from pronase. However, ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
1998 - American Society for Microbiology | Infection and Immunity
Renée E. Collie, John F. Kokai–Kun, Bruce A. McClane,
Clostridium perfringens enterotoxin (CPE) has been implicated as an important virulence factor inC. perfringens type A food poisoning and several non-foodborne human gastrointestinal (GI) illnesses, including antibiotic-associated diarrhea (AAD) and sporadic diarrhea (SPOR). Recent studies have revealed genotypic differences between cpe-positive isolates originating from different disease sources, with most, or all, food poisoning isolates carrying a chomosomal cpe and most, or all, non-foodborne human ...
Tópico(s): Microscopic Colitis
1998 - Elsevier BV | Anaerobe
Dawn M. Bueschel, Richard L. Walker, Leslie Woods, John F. Kokai–Kun, Bruce A. McClane, J. Glenn Songer,
A Thoroughbred-Quarter Horse crossbred foal developed hemorrhagic enteritis and died < 48 hours after birth. Gross and histologic findings were suggestive of Clostridium perfringens type C infection, and large numbers of C perfringens were isolated from intestinal contents. However, genotyping of isolates indicated that they were enterotoxigenic C perfringens type A, and isolates were found to produce C perfringens enterotoxin in vitro. This case suggests that enterotoxigenic C perfringens type A ...
Tópico(s): Clostridium difficile and Clostridium perfringens research
1998 - American Veterinary Medical Association | Journal of the American Veterinary Medical Association
John F. Kokai–Kun, Bruce A. McClane,
To further our knowledge of the structure-function relationship and mechanism of action of the Clostridium perfringens enterotoxin (CPE), a series of recombinant CPE (rCPE) species containing N- and C-terminal CPE deletion fragments was constructed by recombinant DNA approaches. Each rCPE species was characterized for its ability to complete the first four early steps in the action of CPE, putatively ordered as specific binding, a postbinding physical change to bound CPE, large-complex formation, ...
Tópico(s): Bacterial Genetics and Biotechnology
1997 - American Society for Microbiology | Infection and Immunity
John F. Kokai–Kun, Bruce A. McClane,
Tópico(s): Botulinum Toxin and Related Neurological Disorders
1997 - Oxford University Press | Clinical Infectious Diseases
John F. Kokai–Kun, Bruce A. McClane,
In studies performed to investigate the topology of Clostridium perfringens enterotoxin (CPE) when this toxin is associated with intestinal brush border membrane (BBMs), it was shown that radiolabeled CPE antibodies react more strongly against intact CPE-treated BBMs than against control BBMs. Immunoprecipitation studies then demonstrated that CPE antibodies are able to react with both small and large CPE-containing complexes while these complexes are still present in intact BBMs. Therefore, at least ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
1996 - American Society for Microbiology | Infection and Immunity
Thomas E. Hamilton, Steven J. McClane, H. Scott Baldwin, Charlotte Burke, Hitendra Patel, John L. Rombeau, Steven E. Raper,
Clinical application of gene therapy for patients who have inflammatory bowel disease or short bowel syndrome will require the development of new strategies to improve the efficiency of small intestinal gene transfer. Previously, the authors developed a method for adenoviral-mediated small intestinal gene transfer in vivo in neonatal and adult mice. The present study evaluates the hypothesis that the integrins alpha(v)beta3 and alpha(v)beta5, the secondary receptors for adenoviral internalization, ...
Tópico(s): Cancer Research and Treatments
1997 - Elsevier BV | Journal of Pediatric Surgery
Francisco A. Uzal, Mauricio A. Navarro, Jihong Li, John C. Freedman, Archana Shrestha, Bruce A. McClane,
Several enteric clostridial diseases can affect humans and animals. Of these, the enteric infections caused by Clostridium perfringens and Clostridium difficile are amongst the most prevalent and they are reviewed here. C. perfringens type A strains encoding alpha toxin (CPA) are frequently associated with enteric disease of many animal mammalian species, but their role in these diseased mammals remains to be clarified. C. perfringens type B encoding CPA, beta (CPB) and epsilon (ETX) toxins causes ...
Tópico(s): Gut microbiota and health
2018 - Elsevier BV | Anaerobe
John C. Freedman, Archana Shrestha, Bruce A. McClane,
Clostridium perfringens enterotoxin (CPE) is responsible for causing the gastrointestinal symptoms of several C. perfringens food- and nonfood-borne human gastrointestinal diseases. The enterotoxin gene (cpe) is located on either the chromosome (for most C. perfringens type A food poisoning strains) or large conjugative plasmids (for the remaining type A food poisoning and most, if not all, other CPE-producing strains). In all CPE-positive strains, the cpe gene is strongly associated with insertion ...
Tópico(s): Barrier Structure and Function Studies
2016 - Multidisciplinary Digital Publishing Institute | Toxins
Jorge P. García, Jihong Li, Archana Shrestha, John C. Freedman, Juliann Beingesser, Bruce A. McClane, Francisco A. Uzal,
ABSTRACT Clostridium perfringens enterotoxin causes the gastrointestinal (GI) symptoms of C. perfringens type A food poisoning and CPE-associated non-food-borne human GI diseases. It is well established that CPE induces fluid accumulation and severe tissue damage in ligated small intestinal loops of rabbits and other animals. However, a previous study had also reported that CPE binds to rabbit colonic cells yet does not significantly affect rabbit colonic loops. To the contrary, the current study ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
2014 - American Society for Microbiology | Infection and Immunity
John F. Kokai–Kun, J. Glenn Songer, John R. Czeczulin, Fei Chen, Bruce A. McClane,
Clostridium perfringens enterotoxin (CPE) is an important sporulation-associated virulence factor in several illnesses of humans and domestic animals, including C. perfringens type A food poisoning. Therefore, the ability to determine the enterotoxigenicity of food or fecal C. perfringens isolates with simple, rapid assays should be helpful for epidemiologic investigations. In this study, Western immunoblotting (to detect CPE production in vitro) was compared with PCR assays and digoxigenin-labeled ...
Tópico(s): Antimicrobial Resistance in Staphylococcus
1994 - American Society for Microbiology | Journal of Clinical Microbiology
Jihong Li, John C. Freedman, Daniel R. Evans, Bruce A. McClane,
Clostridium perfringens type D strains cause enterotoxemia and enteritis in livestock via epsilon toxin production. In type D strain CN3718, CodY was previously shown to increase the level of epsilon toxin production and repress sporulation. C. perfringens type A strains producing C. perfringens enterotoxin (CPE) cause human food poisoning and antibiotic-associated diarrhea. Sporulation is critical for C. perfringens type A food poisoning since spores contribute to transmission and resistance in ...
Tópico(s): Antimicrobial Resistance in Staphylococcus
2017 - American Society for Microbiology | Infection and Immunity