Pablo C. Baldi, Silvia E. Miguel, Carlos A. Fossati, Jorge C. Wallach,
A serological 2-year follow-up of 35 patients who had brucellosis with different clinical outcomes was performed. Patients were followed up by standard tube agglutination (STA) and 2-mercaptoethanol STA (2ME-STA) tests and by two enzyme-linked immunosorbent assays (ELISAs) to independently measure values of serum IgG to either lipopolysaccharide (LPS) or cytoplasmic proteins of Brucella. Whereas STA and 2ME-STA tests revealed characteristic antibody profiles for patients who recovered (group I), this ...
Tópico(s): Whipple's Disease and Interleukins
1996 - Oxford University Press | Clinical Infectious Diseases
Silvia Fossati, Jorge Ghiso, Agueda Rostagno,
Vascular deposition of amyloid-β (Aβ) in sporadic and familial Alzheimer’s disease, through poorly understood molecular mechanisms, leads to focal ischemia, alterations in cerebral blood flow, and cerebral micro-/macro-hemorrhages, significantly contributing to cognitive impairment. Here, we show that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptors DR4 and DR5 specifically mediate oligomeric Aβ induction of extrinsic apoptotic pathways in human microvascular cerebral ...
Tópico(s): Trace Elements in Health
2012 - Springer Nature | Cell Death and Disease
Silvia Fossati, Jorge Ghiso, Agueda Rostagno,
<i>Background:</i> The vascular deposition of amyloid known as cerebral amyloid angiopathy (CAA) – an age-associated condition and a common finding in Alzheimer’s disease – compromises cerebral blood flow, causing macro/microhemorrhages and/or cognitive impairment. Very little is known about the mechanisms causing CAA-related degeneration of cerebral vascular cells. The Dutch E22Q familial amyloid-β (Aβ) variant is primarily associated with CAA, and manifests clinically with severe cerebral ...
Tópico(s): Cerebrovascular and genetic disorders
2011 - Karger Publishers | Neurodegenerative Diseases
Ricardo J.S. Viana, Ana F. Nunes, Rui E. Castro, Rita M. Ramalho, Jordana L. Meyerson, Silvia Fossati, Jorge Ghiso, Agueda Rostagno, Cecília M. P. Rodrigues,
The vasculotropic E22Q mutant of the amyloid-β (Aβ) peptide is associated with hereditary cerebral hemorrhage with amyloidosis Dutch type. The cellular mechanism(s) of toxicity and nature of the AβE22Q toxic assemblies are not completely understood. Comparative assessment of structural parameters and cell death mechanisms elicited in primary human cerebral endothelial cells by AβE22Q and wild-type Aβ revealed that only AβE22Q triggered the Bax mitochondrial pathway of apoptosis. AβE22Q neither matched ...
Tópico(s): S100 Proteins and Annexins
2009 - Springer Nature | Cellular and Molecular Life Sciences
Krysti Todd, Silvia Fossati, Jorge Ghiso, Agueda Rostagno,
Familial British dementia (FBD) is an early-onset non-amyloid-β (Aβ) cerebral amyloidosis that presents with severe cognitive decline and strikingly similar neuropathological features to those present in Alzheimer's disease (AD). FBD is associated with a T to A single nucleotide transition in the stop codon of a gene encoding BRI2, leading to the production of an elongated precursor protein. Furin-like proteolytic processing at its C-terminus releases a longer-than-normal 34 amino acid peptide, ABri, ...
Tópico(s): S100 Proteins and Annexins
2014 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Produção Nacional
Revisado por pares
Gabriela Sentena Severino, Isabel Amaral Martins Fossati, Maristela Jorge Padoin, Coelho Gomes, Luciano Trevizan, Gilberto Luiz Sanvitto, Celso Rodrigues Franci, Janet A. Anselmo-Franci, Aldo Bolten Lucion,
Neonatal handling induces behavioral and hormonal changes, characterized by reduced fear in novel environments, and lesser elevation and faster return to basal levels of plasma corticosterone, prolactin and adrenaline, in response to stressors in adulthood. The present study aimed to analyze the effects of neonatal handling from Days 1 to 10 postnatal on prolactin response to ether stress in male and female rats at three life periods: neonatal, peripubertal and adulthood. Moreover, adult females ...
Tópico(s): Adipose Tissue and Metabolism
2004 - Elsevier BV | Physiology & Behavior
Silvia Fossati, Krysti Todd, Krystal Sotolongo, Jorge Ghiso, Agueda Rostagno,
Mutations within the Aβ (amyloid β) peptide, especially those clustered at residues 21-23, are linked to early-onset AD (Alzheimer's disease) and primarily associated with cerebral amyloid angiopathy. The Iowa variant, a substitution of an aspartic acid residue for asparagine at position 23 (D23N), associates with widespread vascular amyloid and abundant diffuse pre-amyloid lesions significantly exceeding the incidence of mature plaques. Brain Iowa deposits consist primarily of a mixture of mutated ...
Tópico(s): Cholinesterase and Neurodegenerative Diseases
2013 - Portland Press | Biochemical Journal
Produção Nacional
Revisado por pares
Gustavo Graciano Fonseca, Eliézer Ávila Gandra, Liana Fossati Sclowitz, Ana Paula Antunes Corrêa, Jorge Alberto Vieira Costa,
Tópico(s): Phytochemicals and Antioxidant Activities
2008 - Springer Science+Business Media | World Journal of Microbiology and Biotechnology
Raffaella Solito, Federico Corti, Silvia Fossati, Emiliya Mezhericher, Sandra Donnini, Jorge Ghiso, Antonio Giachetti, Agueda Rostagno, Marina Ziche,
Single point mutations of the amyloid precursor protein generate Abeta variants bearing amino acid substitutions at positions 21-23. These mutants are associated with distinct hereditary phenotypes of cerebral amyloid angiopathy, manifesting varying degrees of tropism for brain vessels, and impaired microvessel remodeling and angiogenesis. We examined the differential effects of E22Q (Dutch), and E22G (Arctic) variants in comparison to WT Abeta on brain endothelial cell proliferation, angiogenic phenotype ...
Tópico(s): Cerebrovascular and genetic disorders
2008 - Elsevier BV | Experimental Cell Research
Jorge Ghiso, Silvia Fossati, Agueda Rostagno,
Substantial genetic, biochemical, and in vivo data indicate that progressive accumulation of amyloid-β (Aβ) plays a central role in the pathogenesis of Alzheimer's disease (AD). Historically centered in the importance of parenchymal plaques, the role of cerebral amyloid angiopathy (CAA)--a frequently neglected amyloid deposit present in >80% of AD cases--for the mechanism of disease pathogenesis is now starting to emerge. CAA consistently associates with microvascular modifications, ischemic lesions, ...
Tópico(s): Amyloidosis: Diagnosis, Treatment, Outcomes
2014 - IOS Press | Journal of Alzheimer s Disease
Gustavo Graciano Fonseca, Eliezer Avila Gand, Liana Fossati S, Ana Paula Antunes Co, Jorge Alberto, Jose Alberto Le,
Tópico(s): Enzyme-mediated dye degradation
2007 - | International Journal of Plant Breeding and Genetics
Pablo C. Baldi, George F. Araj, Graciela C. Racaro, Jorge C. Wallach, Carlos A. Fossati,
ABSTRACT The diagnosis of human neurobrucellosis usually relies on the detection of antibodies to Brucella lipopolysaccharide (LPS) in cerebrospinal fluid (CSF) by agglutination tests or enzyme-linked immunosorbent assay (ELISA). Here we describe the detection of immunoglobulin G (IgG) to cytoplasmic proteins (CP) of Brucella spp. by ELISA and Western blotting in seven CSF samples from five patients with neurobrucellosis. While IgG to CP (titers of 200 to 12,800) and IgG to LPS (800 to 6,400) were found ...
Tópico(s): Whipple's Disease and Interleukins
1999 - American Society for Microbiology | Clinical and Diagnostic Laboratory Immunology
Produção Nacional
Revisado por pares
Pauline Delaveau, Tiago Arruda Sanchez, Ricardo Ewbank Steffen, Karine Deschet, Maritza Jabourian, Vincent Perlbarg, Emerson L. Gasparetto, Stéphanie Dubal, Jorge Costa e Silva, Philippe Fossati,
Abstract Clinical remission of depression may be associated with emotional residual symptoms. We studied the association of emotional blunting, rumination with neural networks dynamics in remitted depressed patients and cognitive performance during an N‐Back task. Twenty‐six outpatients in remission of depression (Hamilton Depressive rating scale score <7) performed an N‐Back task during fMRI assessment. All patients had been treated by paroxetine for a minimum of 4 months. Two subgroups of patients [ ...
Tópico(s): Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
2017 - Wiley | Human Brain Mapping
Renata Curciarello, Jorge Fabricio Lareu, Carlos A. Fossati, Guillermo Docena, Silvana Petruccelli,
Summary Background Cows' milk allergy (CMA) is the most common cause of food allergy in infancy. The only proven treatment is the complete elimination of cows' milk proteins (CMPs) from the diet by means of hypoallergenic formulas. Soybean‐based formulae are widely used although intolerance to soy has been reported to occur in 15–40% of infants with CMA. Objective The aim of this work was to analyse the in vitro reactivity of the soybean cultivar Raiden, which naturally lacks glycinin A 4 A 5 B 3 , ...
Tópico(s): Allergic Rhinitis and Sensitization
2008 - Wiley | Clinical & Experimental Allergy
Paula Barrionuevo, Juliana Cassataro, M. Victoria Delpino, Astrid Zwerdling, Karina A. Pasquevich, Clara García Samartino, Jorge C. Wallach, Carlos A. Fossati, Guillermo H. Giambartolomei,
The strategies that allow Brucella abortus to survive inside macrophages for prolonged periods and to avoid the immunological surveillance of major histocompatibility complex class II (MHC-II)-restricted gamma interferon (IFN-gamma)-producing CD4+ T lymphocytes are poorly understood. We report here that infection of THP-1 cells with B. abortus inhibited expression of MHC-II molecules and antigen (Ag) processing. Heat-killed B. abortus (HKBA) also induced both these phenomena, indicating the independence ...
Tópico(s): vaccines and immunoinformatics approaches
2007 - American Society for Microbiology | Infection and Immunity
Juliana Cassataro, Karina A. Pasquevich, Laura Bruno, Jorge C. Wallach, Carlos A. Fossati, Pablo C. Baldi,
ABSTRACT Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella abortus . Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We obtained recombinant purified Omp31 ( B. melitensis ) and tested its recognition by sera from humans and ...
Tópico(s): Galectins and Cancer Biology
2004 - American Society for Microbiology | Clinical and Vaccine Immunology
Fernando A. Goldbaum, Juliana Leoni, Jorge C. Wallach, Carlos A. Fossati,
Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice immunized by infection with Brucella ovis cells have been obtained. One of these MAbs, BI24, was used to purify by immunoaffinity a protein with a pI of 5.6 and a molecular mass of 18 kDa. This protein was present in all of the rough and smooth Brucella species studied, but it could not be detected in Yersinia enterocolitica 09. Three internal peptides of this protein were partially sequenced; no homology with other bacterial proteins ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1993 - American Society for Microbiology | Journal of Clinical Microbiology
Silvia Fossati, Patrizia Giannoni, María E. Solesio, Sarah L. Cocklin, Erwin Cabrera, Jorge Ghiso, Agueda Rostagno,
Mitochondrial dysfunction has been recognized as an early event in Alzheimer's disease (AD) pathology, preceding and inducing neurodegeneration and memory loss. The presence of cytochrome c (CytC) released from the mitochondria into the cytoplasm is often detected after acute or chronic neurodegenerative insults, including AD. The carbonic anhydrase inhibitor (CAI) methazolamide (MTZ) was identified among a library of drugs as an inhibitor of CytC release and proved to be neuroprotective in Huntington' ...
Tópico(s): Mitochondrial Function and Pathology
2015 - Elsevier BV | Neurobiology of Disease
Rebecca Parodi‐Rullán, Jorge Ghiso, Erwin Cabrera, Agueda Rostagno, Silvia Fossati,
Impaired clearance in the Alzheimer's Disease (AD) brain is key in the formation of Aβ parenchymal plaques and cerebrovascular deposits known as cerebral amyloid angiopathy (CAA), present in >80% of AD patients and ~50% of non-AD elderly subjects. Aβ deposits are highly heterogeneous, containing multiple fragments mostly derived from catabolism of Aβ40/Aβ42, which exhibit dissimilar aggregation properties. Remarkably, the role of these physiologically relevant Aβ species in cerebrovascular injury and ...
Tópico(s): Nuclear Receptors and Signaling
2020 - Wiley | Aging Cell
Silvia Fossati, Judy Cam, Jordana L. Meyerson, Emiliya Mezhericher, Ignacio A. Romero, P. O. Couraud, Babette B. Weksler, Jorge Ghiso, Agueda Rostagno,
Cerebral amyloid angiopathy (CAA) is an age-associated condition and a common finding in Alzheimer's disease in which amyloid-β (Aβ) vascular deposits are featured in >80% of the cases. Familial Aβ variants bearing substitutions at positions 21–23 are primarily associated with CAA, although they manifest with strikingly different clinical phenotypes: cerebral hemorrhage or dementia. The recently reported Piedmont L34V Aβ mutant, located outside the hot spot 21–23, shows a similar hemorrhagic phenotype, ...
Tópico(s): Intracerebral and Subarachnoid Hemorrhage Research
2009 - Wiley | The FASEB Journal
Sepehr Mortaheb, Alice Barra, Manon Carrière, Mariachiara Binda Fossati, Géraldine Martens, Yelena G. Bodien, Jorge Leon Morales-Quezada, Felipe Fregni, Joseph T. Giacino, Steven Laureys, Aurore Thibaut,
... Mortaheb Alice Barra Manon Carrière Mariachiara L Binda Fossati Geraldine Martens Yelena Bodien Jorge L Morales-Quezada Felipe Fregni Joseph Giacino Steven ... Mortaheb Alice Barra Manon Carrière Mariachiara L Binda Fossati Geraldine Martens Yelena Bodien Jorge L Morales-Quezada Felipe Fregni Joseph Giacino Steven ... Mortaheb Alice Barra Manon Carrière Mariachiara L Binda Fossati Geraldine Martens Yelena Bodien Jorge L Morales-Quezada Felipe Fregni Joseph Giacino Steven ... Mortaheb Alice Barra Manon Carrière Mariachiara L Binda Fossati Geraldine Martens Yelena Bodien Jorge L Morales-Quezada Felipe Fregni Joseph Giacino Steven ...
Tópico(s): EEG and Brain-Computer Interfaces
2018 - Frontiers Media | Frontiers in Neuroscience
Mar Hernández‐Guillamón, S. Mawhirt, Silvia Fossati, Steven P. Blais, Mireia Parés, Anna Peñalba, Merçé Boada, Pierre‐Olivier Couraud, Thomas A. Neubert, Joan Montaner, Jorge Ghiso, Agueda Rostagno,
Patients carrying mutations within the amyloid-beta (Abeta) sequence develop severe early-onset cerebral amyloid angiopathy with some of the related variants manifesting primarily with hemorrhagic phenotypes. Matrix metalloproteases (MMPs) are typically associated with blood brain barrier disruption and hemorrhagic transformations after ischemic stroke. However, their contribution to cerebral amyloid angiopathy-related hemorrhage remains unclear. Human brain endothelial cells challenged with Abeta ...
Tópico(s): S100 Proteins and Annexins
2010 - Elsevier BV | Journal of Biological Chemistry
Erika Mogni, Giovanni Pellegrini, Jorge Gil‐Rostra, F. Yubero, Giuseppina Simone, Stefan Fossati, Jakub Dostálek, Rebeca Martínez Vázquez, Roberto Osellame, Michele Celebrano, Marco Finazzi, Paolo Biagioni,
Abstract Bloch surface waves sustained by truncated 1D photonic crystals (1DPCs) are well known tools for surface‐enhanced spectroscopy. They provide strongly confined fields with uniform distribution over a large surface area, a characteristic exploited in standard refractometric sensing. However, their application to polarization‐sensitive investigations is not straightforward because the transverse electric (TE) and magnetic (TM) surface modes possess distinct dispersion relations, therefore their ...
Tópico(s): Plasmonic and Surface Plasmon Research
2022 - Wiley | Advanced Optical Materials
Libby Wood, Guillaume Bassez, Corinne Bleyenheuft, Craig Campbell, Louise Cossette, Cecilia Jimenez‐Moreno, Yi Dai, Hugh Dawkins, Jorge Alberto Diaz Manera, Céline Dogan, Rasha El Sherif, Barbara Fossati, Caroline Graham, James E. Hilbert, Kristina Kastreva, En Kimura, Lawrence Korngut, Anna Kostera‐Pruszczyk, Christopher Lindberg, Björn Lindvall, Elizabeth Luebbe, Anna Łusakowska, Radim Mazanec, Giovani Meola, Liannna Orlando, Masanori Takahashi, Stojan Perić, Jack Puymirat, Vidosava Rakočević-Stojanović, Miriam Rodrigues, Richard Roxburgh, Benedikt Schoser, Sonia Segovia, Andriy Shatillo, Simone Thiele, Ivailo Tournev, Baziel G.M. van Engelen, Stanislav Voháňka, Hanns Lochmüller,
Myotonic Dystrophy is the most common form of muscular dystrophy in adults, affecting an estimated 10 per 100,000 people. It is a multisystemic disorder affecting multiple generations with increasing severity. There are currently no licenced therapies to reverse, slow down or cure its symptoms. In 2009 TREAT-NMD (a global alliance with the mission of improving trial readiness for neuromuscular diseases) and the Marigold Foundation held a workshop of key opinion leaders to agree a minimal dataset ...
Tópico(s): Genetic Neurodegenerative Diseases
2018 - BioMed Central | Orphanet Journal of Rare Diseases
Jorge C. Wallach, Mariana C. Ferrero, M. Victoria Delpino, Carlos A. Fossati, Pablo C. Baldi,
The pathological consequences of exposure to the vaccine strain Brucella abortus S19 were evaluated in 30 employees from vaccine-manufacturing plants. Active brucellosis was diagnosed in 21 subjects, of whom only five recalled an accidental exposure. Clinical manifestations were mild, and only one patient presented a complication. After antimicrobial therapy, initially symptomatic patients either experienced clinical remission or had mild persistent symptoms. This is the first study reporting infection ...
Tópico(s): Animal Disease Management and Epidemiology
2008 - Elsevier BV | Clinical Microbiology and Infection
Jorge C. Wallach, Guillermo H. Giambartolomei, Pablo C. Baldi, Carlos A. Fossati,
Abstract The less mucoid strain of Brucella canis or M- strain is used for the serologic diagnosis of canine brucellosis. While this strain is avirulent in dogs, we report the case of clinical brucellosis that developed in a laboratory worker a few days after handling live M- cells for antigen production.
Tópico(s): Galectins and Cancer Biology
2004 - Centers for Disease Control and Prevention | Emerging infectious diseases
Guillermo H. Giambartolomei, M. Victoria Delpino, Mariela E. Cahanovich, Jorge C. Wallach, Pablo C. Baldi, Carlos A. Velikovsky, Carlos A. Fossati,
This study evaluated the cellular immune response against Brucella species cytoplasmic protein (CP) in peripheral blood mononuclear cells (PBMC) of 25 patients with brucellosis. In vitro proliferation and cytokine gene expression and production were investigated. PBMC from 14 patients proliferated in response to CP (responder patients [RPs]) and cells from 11 patients did not (nonresponder patients [NRPs]). CP-specific interleukin (IL)–2 and interferon-γ were significantly induced in PBMC from RPs, compared ...
Tópico(s): Burkholderia infections and melioidosis
2002 - Oxford University Press | The Journal of Infectious Diseases
Fernando A. Goldbaum, Carlos P. Rubbi, Jorge C. Wallach, Silvia E. Miguel, Pablo C. Baldi, Carlos A. Fossati,
A preparation of Brucella abortus cytoplasmic proteins was depleted of lipopolysaccharide (LPS) by immunoadsorption with a monoclonal antibody (MAb), BC68, specific for the O antigen of B. abortus smooth LPS. Two enzyme-linked immunosorbent assay (ELISA) systems were developed and used in this study. The first system includes an LPS-free cytoplasmic protein preparation; the second one was based on antigen capture on MAb BC68. By using these systems, we have demonstrated that 94% (33 of 35) of the brucellosis ...
Tópico(s): Galectins and Cancer Biology
1992 - American Society for Microbiology | Journal of Clinical Microbiology
Pablo C. Baldi, Guillermo H. Giambartolomei, Jorge C. Wallach, Carlos A. Velikovsky, Carlos A. Fossati,
AbstractAntibodies to cytoplasmic proteins (CP) of Brucella have been shown to be useful for the diagnosis of human brucellosis; however, some early-diagnosed patients lack such an antibody response while having high titers of antibodies to lipopolysaccharide (LPS). To address which factors determine this serological discrepancy in the early stages of brucellosis we examined the antibody response to CP and LPS of 21 patients involved in an outbreak of B. melitensis infection who had a short duration ...
Tópico(s): Leptospirosis research and findings
2001 - Informa | Scandinavian Journal of Infectious Diseases
Jorge C. Wallach, M. Victoria Delpino, Romina Scian, Bettina Deodato, Carlos A. Fossati, Pablo C. Baldi,
A case of prepatellar bursitis in a man with chronic brucellosis is presented. Brucella abortus biotype 1 was isolated from the abundant yellowish fluid obtained from the bursa. Clinical and epidemiological data did not suggest a direct inoculation of the agent in the bursa. However, the patient mentioned occasional local trauma due to recreational sports, which may have constituted a predisposing factor. As determined by ELISA, there were higher levels of IgG against Brucella LPS and cytosolic ...
Tópico(s): Musculoskeletal synovial abnormalities and treatments
2010 - Microbiology Society | Journal of Medical Microbiology