Produção Nacional
Revisado por pares
Rubén J. Sánchez, Jan Schripsema, Luiziana Ferreira da Silva, Marilda Keico Taciro, José Geraldo da Cruz Pradella, José Gregório Cabrera Gomez,
Medium-chain-length polyhydroxyalkanoates are produced by Pseudomonas putida strain IPT046 growing on carbohydrates. Analysis by gas chromatography and nuclear magnetic resonance of the elastomeric material revealed that PHAmcl is composed from essentially hydroxydecanoate (60–70%) and hydroxyoctanoate (20–25%) sequence units with a non-terminal double bond in about 6% of the side chains. The average molecular weight of PHAmcl is 223 kDalton and the X-ray diffractogram showed that 24% of the solid ...
Tópico(s): Microplastics and Plastic Pollution
2003 - Elsevier BV | European Polymer Journal
Produção Nacional
Revisado por pares
Luiziana Ferreira da Silva, Marilda Keico Taciro, M. E. Michelin Ramos, J. M. Carter, José Geraldo da Cruz Pradella, José Gregório Cabrera Gomez,
Fifty-five bacterial strains isolated from soil were screened for efficient poly-3-hydroxybutyrate (P3HB) biosynthesis from xylose. Three strains were also evaluated for the utilization of bagasse hydrolysate after different detoxification steps. The results showed that activated charcoal treatment is pivotal to the production of a hydrolysate easy to assimilate. Burkholderia cepacia IPT 048 and B. sacchari IPT 101 were selected for bioreactor studies, in which higher polymer contents and yields ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2004 - Springer Science+Business Media | Journal of Industrial Microbiology & Biotechnology
Produção Nacional
Revisado por pares
José Geraldo Pereira da Cruz, Tainaê Santos, Monaliza Marizete Baungratz, Júlia Niehues da Cruz, Débora Delwing Dal Magro, José Geraldo Pereira da Cruz,
Simvastatin inhibits 3-hydroxy-3-methylglutaryl CoA reductase, the rate-limiting enzyme in the cholesterol biosynthetic pathway, and is widely used to control plasma cholesterol levels and prevent cardiovascular disease. However, emerging evidence indicates that the beneficial effects of simvastatin extend to the central nervous system. The effects of simvastatin combined with fluoxetine provide an exciting and potential paradigm to decreased anxiety and depression. Thus, the present paper investigates ...
Tópico(s): Treatment of Major Depression
2012 - Dove Medical Press | Neuropsychiatric Disease and Treatment
Produção Nacional
Revisado por pares
Laísa dos Reis, Roselei Claudete Fontana, Priscila da Silva Delabona, Deise Juliana da Silva Lima, Marli Camassola, José Geraldo da Cruz Pradella, Aldo José Pinheiro Dillon,
The development of more productive strains of microorganisms and processes that increase enzyme levels can contribute to the economically efficient production of second generation ethanol. To this end, cellulases and xylanases were produced with the S1M29 mutant strain of Penicillium echinulatum, using different concentrations of cellulose (20, 40, and 60 g L−1) in batch and fed-batch processes. The highest activities of FPase (8.3 U mL−1), endoglucanases (37.3 U mL−1), and xylanases (177 U mL−1) were ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2013 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
S. R. Silva-Queiroz, Luiziana Ferreira da Silva, José Geraldo da Cruz Pradella, E.M. Pereira, José Gregório Cabrera Gomez,
The production of PHA from plant oils by Pseudomonas species soil isolated from a sugarcane crop was evaluated. Out of 22 bacterial strains three were able to use efficiently plant oils to grow and to accumulate PHA. Pseudomonas putida and Pseudomonas aeruginosa strains produced PHA presenting differences on monomer composition compatible with variability on monomer specificity of their PHA biosynthesis system. The molar fraction of 3-hydroxydodecanoate detected in the PHA was linearly correlated ...
Tópico(s): Enzyme Catalysis and Immobilization
2009 - Elsevier BV | Journal of Biotechnology
Produção Nacional
Revisado por pares
Lana Zanetti Tavares, Elda Sabino da Silva, José Geraldo da Cruz Pradella,
The influence of different aeration rates (Qair), 12, 20, 30, 35, 40, and 50 l min−1, on the production of poly(3-hydroxybutyric acid) (PHB) by Ralstonia eutropha DSM 545 during the accumulation phase, was investigated in an airlift bioreactor and the results were compared to the ones obtained in a stirred tank bioreactor. The time constants for mixing, oxygen transfer, oxygen consumption, power consumption, and kinetic parameters were the tools used to compare the systems. The results showed that, ...
Tópico(s): Enzyme Catalysis and Immobilization
2003 - Elsevier BV | Biochemical Engineering Journal
Produção Nacional
Revisado por pares
José Geraldo da Cruz Pradella, Marilda Keico Taciro, Alis Pataquiva-Mateus,
Burkholderia sacchari IPT 189 poly (3-hydroxybutyrate) (P3HB) production in airlift bioreactor were investigated in batch and fed-batch culture using sucrose as carbon source. In batch experiments it was observed that during the growth phase B. sacchari IPT 189 might display exponential growth even at very low carbohydrate concentration, as long as NH4+ concentration was above 190 mg l−1. The onset of accumulation phase took place when NH4+ concentration dropped below this value and continued as long ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2010 - Elsevier BV | Bioresource Technology
Produção Nacional
Miriam Lúcia Chiquetto Machado, Nilson Casimiro Pereira, Leila Figueiredo de Miranda, Mauro César Terence, José Geraldo da Cruz Pradella,
O PHB (Poli-3-hidroxibutirato) é um termoplástico biodegradável sintetizado por fermentação submersa a partir de matérias-primas renováveis. A utilização de fibras naturais tem sido pesquisada visando melhorar as propriedades e reduzir o custo do PHB. O objetivo deste trabalho foi o estudo das propriedades mecânicas (resistência à tração), térmicas (Análise termogravimétrica,TGA, e Calorimetria Exploratória Diferencial, DSC) e reológicas (torque em câmera de mistura e Índice de Fluidez) do PHB e de ...
Tópico(s): Polymer crystallization and properties
2010 - Associação Brasileira de Polímeros | Polímeros
Produção Nacional
Revisado por pares
Luiziana Ferreira da Silva, José Gregório Cabrera Gomez, Rafael Costa Santos Rocha, Marilda Keico Taciro, José Geraldo da Cruz Pradella,
BRAZIL FOR BIODEGRADABLE POLYMERS.In recent years, several studies have been developed in Brazil to produce biodegradable materials.A particular family of bacterial polymers, the polyhydroxyalkanoates (PHA), has received special attention.PHAs are thermoplastic, biodegradable, biocompatible, are synthesised from renewable resources and can substitute petrochemical plastics in some applications.Different aspects have been focused to increase productivity and to reduce the cost of PHA production: bacterial ...
Tópico(s): biodegradable polymer synthesis and properties
2007 - Brazilian Chemical Society | Química Nova
Produção Nacional
Revisado por pares
Rafael C. S. Rocha, Luiziana Ferreira da Silva, Marilda Keico Taciro, José Geraldo da Cruz Pradella,
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2007 - Springer Science+Business Media | World Journal of Microbiology and Biotechnology
Produção Nacional
Revisado por pares
Priscila da Silva Delabona, Cristiane S. Farinas, Mateus Ribeiro da Silva, Sindélia Freitas, José Geraldo da Cruz Pradella,
The on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. This study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. Glycosyl hydrolases (FPase, xylanase, and β-glucosidase) were produced using a new strain of Trichoderma harzianum, isolated from the Amazon rainforest and cultivated under different conditions. The influence of the carbon source was first ...
Tópico(s): Enzyme Catalysis and Immobilization
2011 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
Marina O.S. Dias, Marcelo Pereira da Cunha, Charles D.F. Jesus, George J. M. Rocha, José Geraldo da Cruz Pradella, Carlos Eduardo Vaz Rossell, Rubens Maciel Filho, Antonio Bonomi,
Much of the controversy surrounding second generation ethanol production arises from the assumed competition with first generation ethanol production; however, in Brazil, where bioethanol is produced from sugarcane, sugarcane bagasse and trash will be used as feedstock for second generation ethanol production. Thus, second generation ethanol production may be primarily in competition with electricity production from the lignocellulosic fraction of sugarcane. A preliminary technical and economic ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2011 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
Priscila da Silva Delabona, Cristiane S. Farinas, Deise Juliana da Silva Lima, José Geraldo da Cruz Pradella,
This work investigates the glycosyl hydrolase (GH) profile of a new Trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. The influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on GH biosynthesis was assessed using experimental mixture design (EMD). Additionally, the effect of increased component concentrations in culture media selected from the EMD was studied. It was found that that a mixed culture ...
Tópico(s): Enzyme Production and Characterization
2012 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
José Geraldo da Cruz Pradella, Jaciane Lutz Ienczak, Cecília Romero Delgado, Marilda Keico Taciro,
Tópico(s): Biofuel production and bioconversion
2012 - Springer Science+Business Media | Biotechnology Letters
Produção Nacional
Revisado por pares
Daniela A. Ribeiro, Júnio Cota, Thabata M. Alvarez, Fernanda Brüchli, Juliano Bragato, Beatriz Merchel Piovezan Pereira, Bianca Alves Pauletti, George J. Jackson, Maria Teresa Borges Pimenta, M.T. Murakami, Marli Camassola, Roberto Ruller, Aldo José Pinheiro Dillon, José Geraldo da Cruz Pradella, Adriana Franco Paes Leme, Fábio M. Squina,
Plant feedstocks are at the leading front of the biofuel industry based on the potential to promote economical, social and environmental development worldwide through sustainable scenarios related to energy production. Penicillium echinulatum is a promising strain for the bioethanol industry based on its capacity to produce large amounts of cellulases at low cost. The secretome profile of P. echinulatum after grown on integral sugarcane bagasse, microcrystalline cellulose and three types of pretreated ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2012 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Priscila da Silva Delabona, Júnio Cota, Zaira B. Hoffmam, Maria Paula Paixão, Cristiane S. Farinas, João Paulo L. Franco Cairo, Deise Juliana da Silva Lima, Fábio M. Squina, Roberto Ruller, José Geraldo da Cruz Pradella,
Supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. This study investigated which enzymes were complementary to the enzyme set of Trichoderma harzianum in the degradation of sugarcane bagasse. Specific activities of T. harzianum extract on different substrates were compared with the extracts of Penicillium echinulatum and Trichoderma reesei, and two commercial cellulase preparations. Complementary ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2012 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
Fernanda M. Kapritchkoff, Alexandre P. Viotti, R. C. P. Alli, Marisa Zuccolo, José Geraldo da Cruz Pradella, Alfredo Eduardo Maiorano, Everson Alves Miranda, Antonio Bonomi,
Polyhydroxybutyrate (PHB) is the most studied among a wide variety of polyhydroxyalkanoates, bacterial biodegradable polymers known as potential substitutes for conventional plastics. This work aimed at evaluating the use of enzymes to recover and purify the PHB produced by Ralstonia eutropha DSM545. Screening experiments allowed the selection of trypsin, bromelain and lysozyme among six enzymes, based on their efficiency in lysing cells of a non-PHB producing R. eutropha strain. Then, process conditions ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2005 - Elsevier BV | Journal of Biotechnology
Produção Nacional
Revisado por pares
Simone Cardoso Diniz, Marilda Keico Taciro, José Gregório Cabrera Gomez, José Geraldo da Cruz Pradella,
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2004 - Springer Science+Business Media | Applied Biochemistry and Biotechnology
Produção Nacional
Revisado por pares
Maria Augusta Crivelente Horta, Renato Vicentini, Priscila da Silva Delabona, Prianda Rios Laborda, Aline Crucello, Sindélia Freitas, Reginaldo Massanobu Kuroshu, Igor Polikarpov, José Geraldo da Cruz Pradella, Anete Pereira de Souza,
Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2014 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Cleiton Márcio Pinto Braga, Priscila da Silva Delabona, Deise Juliana da Silva Lima, Maria Paula Paixão, José Geraldo da Cruz Pradella, Cristiane S. Farinas,
Accessory enzymes that assist biomass degradation could be used to improve the recovery of fermentable sugar for use in biorefineries. In this study, different fungal strains isolated from the Amazon rainforest were evaluated in terms of their ability to produce feruloyl esterase (FAE) and xylanase enzymes, and an assessment was made of the contributions of the enzymes in the hydrolysis of pretreated sugarcane bagasse. In the selection step, screening using plate assays was followed by shake flask ...
Tópico(s): Catalysis for Biomass Conversion
2014 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
Daniele Longo Machado, João Moreira Neto, José Geraldo da Cruz Pradella, Antonio Bonomi, Sarita Cândida Rabelo, Aline Carvalho da Costa,
Abstract Although adsorption is an essential step in the enzymatic hydrolysis of lignocellulosic materials, literature reports controversial results in relation to the adsorption of the cellulolitic enzymes on different biomasses/pretreatments, which makes difficult the description of this phenomenon in hydrolysis mathematical models. In this work, the adsorption of these enzymes on Avicel and sugarcane bagasse pretreated by the hydrothermal bagasse (HB) and organosolv bagasse ( OB ) methods was evaluated. ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2014 - Wiley | Biotechnology and Applied Biochemistry
Produção Nacional
Revisado por pares
Diogo Robl, Patrícia dos Santos Costa, Fernanda Büchli, Deise Juliana da Silva Lima, Priscila da Silva Delabona, Fábio M. Squina, Ida Chapaval Pimentel, Gabriel Padilla, José Geraldo da Cruz Pradella,
The aim of this study was to develop a bioprocess for the production of β-glucosidase and pectinase from the fungus Annulohypoxylon stygium DR47. Media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26 U/mL of pectinase at pH 4.0 and 10.13 U/mL of β-glucosidase at pH 5.0. In addition, the enzymes extracts were able to replace partially Celluclast 1.5L in sugar cane bagasse hydrolysis. Proteomic analysis from A. stygium ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2014 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
João Paulo Fernandes Vieira, Jaciane Lutz Ienczak, Carlos Eduardo Vaz Rossell, José Geraldo da Cruz Pradella, Telma Teixeira Franco,
Tópico(s): Biofuel production and bioconversion
2014 - Springer Science+Business Media | Biotechnology Letters
Produção Nacional
Revisado por pares
Jaciane Lutz Ienczak, Mélodi Schmidt, Luci Kelin Quines, Kellen Zanfonato, José Geraldo da Cruz Pradella, Willibaldo Schmidell, Gláucia Maria Falcão de Aragão,
Tópico(s): Enzyme Catalysis and Immobilization
2015 - Springer Science+Business Media | Applied Biochemistry and Biotechnology
Produção Nacional
Revisado por pares
Lucas Gelain, José Geraldo da Cruz Pradella, Aline Carvalho da Costa,
A mathematical model to describe the kinetics of enzyme production by the filamentous fungus Trichoderma harzianum P49P11 was developed using a low cost substrate as main carbon source (pretreated sugarcane bagasse). The model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40 g L−1). The 10 g L−1 concentration was used to validate the model ...
Tópico(s): Enzyme Catalysis and Immobilization
2015 - Elsevier BV | Bioresource Technology
Produção Nacional
Revisado por pares
Diogo Robl, Priscila da Silva Delabona, Patrícia dos Santos Costa, Deise Juliana da Silva Lima, Sarita Cândida Rabelo, Ida Chapaval Pimentel, Fernanda Büchli, Fábio M. Squina, Gabriel Padilla, José Geraldo da Cruz Pradella,
Fungal xylanases have been widely studied and various production methods have been proposed using submerged and solid-state fermentation. This class of enzyme is used to supplement cellulolytic enzyme cocktails in order to enhance the enzymatic hydrolysis of plant cell walls. The present work investigates the production of xylanase and other accessory enzymes by a recently isolated endophytic Aspergillus niger DR02 strain, using the pentose-rich liquor from hydrothermal pretreatment of sugarcane ...
Tópico(s): Enzyme Catalysis and Immobilization
2015 - Taylor & Francis | Biocatalysis and Biotransformation
Produção Nacional
Revisado por pares
João Paulo Fernandes Vieira, Jaciane Lutz Ienczak, Paolo Costa, Carlos Eduardo Vaz Rossell, Telma Teixeira Franco, José Geraldo da Cruz Pradella,
This study aimed to assess the single-cell oil production integrated to a sugarcane-mill and molasses as main raw material. Rhodotorula glutinis lipid production with low-cost raw sugarcane molasses was accessed. The envisage process relies in an optimized sugarcane molasses culture media in a fed-batch strategy to achieve a high-cell density high-lipid cell content. A Process Flow Design (PFD) integrated to a typical Brazilian sugar mill was proposed according to fermentation experiments. Performed ...
Tópico(s): Process Optimization and Integration
2016 - Elsevier BV | Industrial Crops and Products
Produção Nacional
Revisado por pares
H. Brognaro, Thaís Milena de Souza Bezerra, José Geraldo da Cruz Pradella, Priscila da Silva Delabona, Deise Juliana da Silva Lima, Eleni Gomes, Steve Hartson, Janet Rogers, Brian Couger, Rolf A. Prade,
Biomass is abundant, renewable and useful for biofuel production as well as chemical priming for plastics and composites. Deconstruction of biomass by enzymes is perceived as recalcitrant while an inclusive breakdown mechanism remains to be discovered. Fungi such as Myceliophthora thermophila M77 appear to decompose natural biomass sources quite well. This work reports on this fungus fermentation property while producing cellulolytic enzymes using natural biomass substrates. Little hydrolytic activity ...
Tópico(s): Enzyme Production and Characterization
2016 - Springer Nature | AMB Express
Produção Nacional
Priscila Vaz de Arruda, Joana Carolina Freire Sandes Santos, Rita C. L. B. Rodrigues, Débora Danielle Virgínio da Silva, Celina K. Yamakawa, George Jackson de Moraes Rocha, Jonas Nolasco Júnior, José Geraldo da Cruz Pradella, Carlos Eduardo Vaz Rossell, Maria das Graças de Almeida Felipe,
In this study, volumetric oxygen mass transfer coefficient (kLa) was selected as a criterion for facilitating the scale up of xylitol production by Candida guilliermondii at the bench and pilot-scale level. A kLa value of 16 h−1 was applied in reactors with volumetric capacity of 2.4 L, 18 L and 125 L. Fermentation was successfully scaled-up from the bench to pilot-scale level with all experiments demonstrating a minimum of 60% xylose to xylitol conversion efficiency. Under all evaluated conditions ...
Tópico(s): Enzyme Catalysis and Immobilization
2016 - Elsevier BV | Journal of Industrial and Engineering Chemistry
Produção Nacional
Revisado por pares
Priscila da Silva Delabona, Deise Juliana da Silva Lima, Diogo Robl, Sarita Cândida Rabelo, Cristiane S. Farinas, José Geraldo da Cruz Pradella,
Abstract The use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. This work evaluates the production of cellulase enzymes using glycerol for high cell density growth of Trichoderma harzianum followed by induction with a cellulosic material. Firstly, the influence of the carbon source used in the pre-culture step was investigated in ...
Tópico(s): Enzyme Catalysis and Immobilization
2016 - Springer Science+Business Media | Journal of Industrial Microbiology & Biotechnology