Salvatore Di Filippo, Celestina Manzoni, Simeone Andrulli, Giuseppe Pontoriero, Cesare Dell’Oro, Vincenzo La Milia, G. Bacchini, Monica Crepaldi, Maria Carla Bigi, Francesco Locatelli,
... dialysance values underestimated Kde by no more than 4.3 mL/min (P < 0.001). In steps 0 to 1 and 1 to 2, the underestimate of Kde by conventional dialysance increased at higher values of Kde, but this relationship did not exist when considering actual dialysance.The value of ionic dialysance is affected by the method of determination, given the effect of cardiopulmonary recirculation on inlet plasma water conductivity when inlet dialysate conductivity is changed. ...
Tópico(s): Acute Kidney Injury Research
2001 - Elsevier BV | Kidney International
Jihye Ha, Hyeonah Lee, Saeam Shin, Hyunsoo Cho, Haerim Chung, Ji Eun Jang, Soo‐Jeong Kim, June‐Won Cheong, Seung‐Tae Lee, Jin Seok Kim, Jong Rak Choi,
... Vκ-Jκ; IGKB: Vκ-Kde; and JκCκ intron-Kde).‡ NGS analysis was performed with the LymphoTrack IGH FR1 Assay and LymphoTrack IGK Assay (Invivoscribe Technologies).§ Conventional myelomas refer to myeloma with Ig with both heavy and light chain molecules. Open table in a new tab NGS, next-generation sequencing. To examine the correlation between plasma cell percentage and clonality positivity, an analysis was ...
Tópico(s): Glycosylation and Glycoproteins Research
2021 - Elsevier BV | Journal of Molecular Diagnostics
Waleed Alduaij, Laura K. Hilton, Muntadhar Al Moosawi, Susana Ben‐Neriah, Barbara Meissner, Merrill Boyle, Kelly Mekwunye, David W. Scott, Heather A. Leitch, Jeffrey W. Craig,
... no clonal peaks (data not shown), the Vk-Kde/intron-Kde reaction (tube B) identified a single prominent peak ... bp) length amplified by the Vk2f/Vk4/Vk5-Kde primer set,6 consistent with a clonal B-cell population (Vk-Kde 3, Figure 2A, B). The same peak was ... analysis of composite tumor components using IGK Vk-Kde/intron-Kde BIOMED-2 primer sets in (A) the cervical ... shown in the lower panel. The IGK Vk-Kde/intron-Kde reaction yields 3 discrete collections of PCR products ... and their anticipated PCR product size ranges: Vk-Kde 1: Vk1f/6/Vk7-Kde, 210-250 bp, ...
Tópico(s): Viral-associated cancers and disorders
2022 - Wolters Kluwer | HemaSphere
Charu Chaudhry, Matthew C. Weston, Peter Schuck, Christian Rosenmund, Mark L. Mayer,
... type and three mutants. The rate of desensitization (kdes) is fastest for wild type, ∼200-fold slower ... of desensitization relative to wild type (−RT ln kdes/kwt) for each mutant (symbol and error bars). ... produced progressively slower rates of onset of desensitization (kdes) with rank-order responses to 10 mM glutamate ... The most effective combination of mutations, -HERLK-, slowed kdes 190-fold compared with wild-type GluR6, producing ... 298 K for SE and SV experiments, respectively). kdes, kdeact, % des, and krec are the rates of ...
Tópico(s): Cellular transport and secretion
2009 - Springer Nature | The EMBO Journal
Wei Wang, Yi Ding, Andrew Campbell, Beverly C. Handy, L. Jeffrey Medeiros, Pei Lin,
... the lymph node revealed the kappa deleting element (kde), which led to inactivation of the IGK allele; ... dominant IGK clone from lymph node revealed the kde rearranged to the Jk-Ck intron (IGKintr-IGKdel, Fig. 4D). The rearrangement involving kde has been shown to inactivate the IGK allele ... heterogeneous population composed of lymphocytes, plasmacytoid lymphocytes and plasma cells. Similar to other haematopoietic diseases, however, clonal ...
Tópico(s): Lymphoma Diagnosis and Treatment
2019 - Elsevier BV | Pathology
Mark Screen, Per Harald Jonson, Olayinka Raheem, Johanna Palmio, Reijo Laaksonen, Terho Lehtimäki, Mario Sirito, Ralf Krahe, Peter Hackman, Bjarne Udd,
... array raw intensity signals were analyzed with Inforsense KDE version 2.0.4 (Inforsense, London, UK) by ...
Tópico(s): Mitochondrial Function and Pathology
2014 - Elsevier BV | American Journal Of Pathology
Caroline Cox, Michael H. Surgan,
... chapter35_.html [accessed 28 September 2006]. Google ScholarEverett KDE, Dickerson HW. 2003. Ichthyophthirius multifilis and Tetrahymena thermophila ...
Tópico(s): Insect and Pesticide Research
2006 - National Institute of Environmental Health Sciences | Environmental Health Perspectives
Andrey A. Komissarov, Peter A. Andreasen, Julie Støve Bødker, Paul Declerck, John Anagli, Joseph D. Shore,
... large{⇄}k1EIk−2\large{⇄}k2E∼I′kiamp;↗ksamp;↘kdE−I∗↓I∗+E Inhibitory pathwaySubstrate pathway(Eq. 1) ...
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
2004 - Elsevier BV | Journal of Biological Chemistry
... sets: IGHV-IGHJ, IGHD-IGHJ, IGKV-IGKJ, IGKV-KDE/intronRSS-KDE. The forward primer sets in IGHV target the ... the deletion event involves the κ deleting element (Kde) in the intron (intron-Kde), the initial IGK V-J rearrangement is preserved. ... genes have unproductive rearrangements and both undergo intron-Kde–mediated deletion, it is possible to find evidence ... rearrangements per cell, in addition to two intron-Kde rearrangements. In the second case, two IGKVJ rearrangements ... deletion events, one of which was a IGKV-Kde deletion which removes any VJ rearrangement(s) on ...
Tópico(s): Lymphoma Diagnosis and Treatment
2021 - Elsevier BV | Journal of Molecular Diagnostics
Javad Ghorbani, Peter D. Wentzell, Mohsen Kompany‐Zareh, Nematollah Omidikia,
... modulator (MPM) adsorption model parameters such as β, kdes,0, and Keq,0, were fitted to the ... 00 are calculated interquartile range (IQR) for β, kdes,0, and Keq,0, respectively. Finally, results show three and two outliers for β and kdes,0, respectively.
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2022 - Elsevier BV | Journal of Chromatography A
Michaela Nováková, Aleš Janda, Marcin W. Włodarski, Martina Suková, Vít Campr, Markéta Žaliová, Eva Froňková, Elena Vodickova, Ondřej Zapletal, Jan Blatný, Kateřina Lejhancová, Dagmar Pospı́šilová, Zdena Černá, Tomáš Kuhn, Zuzana Zemanová, Jan Trka, Ondřej Hrušák, Jan Starý, Ester Mejstříková,
... were analyzed by FC. The level of intronRSS-Kde recombination excision circles (KREC) and T-cell receptor ...
Tópico(s): Cystic Fibrosis Research Advances
2014 - Elsevier BV | Blood
Giovanna Piras, Maria Monne, Antonella Uras, Laura Pilo, Luciana Arca, Maurizio Cucca, Angelo Palmas, Anna Calvisi, Annalisa Noli, Marco Murineddu, A. Gabbas,
... analysis. IGHV-J, IGHD-J, IGKV-J, IGKV-KDE, IGKJ-C-INTRON-KDE rearrangements were amplified by PCR and analyzed following ... and the 60% presented the non-productive IGKV-KDE and IGKJ-C-INTRON-KDE rearrangements. Parallel analysis of clonality pattern and chromosomal ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2007 - Elsevier BV | Blood
Lloyd Hutchinson, Jong Seo Lee, Bruce A. Woda, Ediz F. Cosar,
... in three multiplexed PCR reactions to target IGK, KDE and IGL rearrangements. Variable region (V) primers were ... FRIII: 29.7%/100%, IGK: 80.4%/100%, KDE: 25.0%/100%, and IGL: 35.1%/96.8%. The combination of IGH FRIII, IGK, and KDE detected 83.8% (31/37) vs 67.3% ( ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2016 - OMICS Publishing Group | Journal of Molecular Biomarkers & Diagnosis
E. Sokol, V I Vasilyev, Svetlana Smirnova, Т Н Сафонова, S G Palshina, Е Б Родионова, Н В Кокосадзе, А. М. Ковригина,
... Ig kappa chain rearrangements (Vκ-Jk and Vκ-KDE/Intron RSS-KDE) in 1 case. The latter patient didn9t have ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2015 - BMJ | Annals of the Rheumatic Diseases
Elena Fernandez Redondo, Joaquín Martínez‐López, Ramón García‐Sánz, María-Ángeles Montalbán, Pilar Martínez‐Sánchez, Bruno Paiva, María‐Victoria Mateos, Laura Rosiñol, Maria Luisa Martín, Rosa Ayala, Rafael Martínez, Javier López, Adrián Alegre, Joan Besalduch, Joan Bargay, MT Hernández, María Eugenia Sarasquete, Pedro Sánchez‐Godoy, M Callís, Manuela Fernández, Joan Bladé, Jesús F. San Miguel, Juan José Lahuerta,
... IGK) rearrangements K-VJ and Kappa deleting element (KDE). DHJ and light chain rearrangements detected at the ...
Tópico(s): Glycosylation and Glycoproteins Research
2011 - Elsevier BV | Blood