Yasuhiro Yoshimatsu, Ikumi Wakabayashi, Shiori Kimuro, Naoya Takahashi, Kazuki Takahashi, Miho Kobayashi, Nako Maishi, Katarzyna A. Podyma‐Inoue, Kyoko Hida, Kohei Miyazono, Tetsuro Watabe,
Abstract The tumor microenvironment (TME) consists of various components including cancer cells, tumor vessels, cancer‐associated fibroblasts (CAFs), and inflammatory cells. These components interact with each other via various cytokines, which often induce tumor progression. Thus, a greater understanding of TME networks is crucial for the development of novel cancer therapies. Many cancer types express high levels of TGF‐β, which induces endothelial‐to‐mesenchymal transition (EndMT), leading to formation ...
Tópico(s): Cancer Cells and Metastasis
2020 - Wiley | Cancer Science
Yuta Takahashi, Mariana Morales Valencia, Yang Yu, Yasuo Ouchi, Kazuki Takahashi, Maxim N. Shokhirev, Kathryn Lande, April Williams, Chiara Fresia, Masakazu Kurita, Tomoaki Hishida, Kensaku Shojima, Fumiyuki Hatanaka, Estrella Núñez‐Delicado, Concepción Rodrı́guez Esteban, Juan Carlos Izpisúa Belmonte,
Summary Transgenerational epigenetic inheritance in mammals remains a debated subject. Here, we demonstrate that DNA methylation of promoter-associated CpG islands (CGIs) can be transmitted from parents to their offspring in mice. We generated DNA methylation-edited mouse embryonic stem cells (ESCs), in which CGIs of two metabolism-related genes, the Ankyrin repeat domain 26 and the low-density lipoprotein receptor, were specifically methylated and silenced. DNA methylation-edited mice generated by microinjection ...
Tópico(s): RNA modifications and cancer
2023 - Cell Press | Cell
Shingo Kodama, Katarzyna A. Podyma‐Inoue, Toshihiro Uchihashi, Kyoko Kurioka, Hitomi Takahashi, Akinari Sugauchi, Kazuki Takahashi, Toshihiro Inubushi, Mikihiko Kogo, Susumu Tanaka, Tetsuro Watabe,
Melanoma is an aggressive type of cancer originating from the skin that arises from neoplastic changes in melanocytes. Transforming growth factor‑β (TGF‑β) is a pleiotropic cytokine and is known to contribute to melanoma progression by inducing the epithelial‑mesenchymal transition (EMT) program and creating an environment that favors tumor progression. There are three TGF‑β isoforms, TGF‑β1, TGF‑β2 and TGF‑β3, all of which engage in pro‑tumorigenic activities by activating SMAD signaling pathways. ...
Tópico(s): Cancer Cells and Metastasis
2021 - Elsevier BV | Oncology Reports
Nobuaki Matsui, Kazuki Takahashi, Miho Takeichi, Touma Kuroshita, Kaori Noguchi, Kana Yamazaki, Hideaki Tagashira, Kenichi Tsutsui, Hideki Okada, Yuki Kido, Yumiko Yasui, Nobuyuki Fukuishi, Yoshiyasu Fukuyama, Masaaki Akagi,
The therapeutic use of neurotrophic factors to treat neurodegenerative disorders, including Alzheimer's disease, is considered feasible. Magnolol and honokiol, constituents of the Magnolia plant, are small organic compounds with neurotrophic activity. We investigated whether magnolol and honokiol can prevent age-related learning and memory impairment and cholinergic deficits in senescence-accelerated mice (SAM). Magnolol (1, 10 mg/kg) or honokiol (0.1, 1 mg/kg) were orally administered to SAMP8 mice ...
Tópico(s): Pharmacological Effects of Natural Compounds
2009 - Elsevier BV | Brain Research
Kazunari Shinbo, Ken Onishi, Shunsuke Miyabayashi, Kazuki Takahashi, Shigenobu Katagiri, Keizo Kato, Futao Kaneko, Rigoberto C. Advíncula,
Ultrathin films containing water-soluble copper (II) phthalocyanine-3,4′,4″,4″′- tetrasulfonic acid (CuPcTs) and poly(diallyldimethylammonium chloride) (PDADMAC) were fabricated using the electrostatic layer-by-layer deposition technique. UV–vis absorption spectra and ellipsometry measurements suggested successive linear depositions of the bilayers of CuPcTs and PDADMAC. The thickness of the CuPcTs monolayer obtained from the ellipsometry was smaller than the plain size of CuPcTs and the molecule planes ...
Tópico(s): Polymer Surface Interaction Studies
2003 - Elsevier BV | Thin Solid Films
Kazuki Takahashi, Takaaki Ishigure, Yasuhiro Koike,
Modal dispersion in plastic optical fibers with W-shaped refractive index profiles (W-shaped POFs) is investigated. The authors focus on the effect of the refractive index valley at the boundary of core and cladding on the group delay. In this paper, particularly, the relationship between the depth of the index valley and the modal dispersion is highlighted. In order to analyze the relationship precisely, the authors prepare the W-shaped POFs with a high numerical aperture (NA /spl sim/ 0.25), which ...
Tópico(s): Advanced Photonic Communication Systems
2006 - Institute of Electrical and Electronics Engineers | Journal of Lightwave Technology
Takaaki Ishigure, H. Endo, K. Ohdoko, Kazuki Takahashi, Yasuhiro Koike,
A plastic optical fiber (POF) having a W-shaped refractive index profile (W-shaped POF) was prepared for the first time that had a possibility to realize a higher bit rate transmission than those of the conventional silica-based multimode fiber and graded index (GI) POF links. Since the W-shaped POF has a valley of the refractive index at the boundary of the core and cladding of the conventional graded-index (GI) POF, the group delay of higher order modes is strongly influenced, and the modal dispersion ...
Tópico(s): Photonic and Optical Devices
2005 - Institute of Electrical and Electronics Engineers | Journal of Lightwave Technology
Kazuki Takahashi, Tomoya Kotani, Yoshinao Katsu, Masakane Yamashita,
In immature zebrafish oocytes, dormant cyclin B1 mRNAs localize to the animal polar cytoplasm as aggregates. After hormonal stimulation, cyclin B1 mRNAs are dispersed and translationally activated, which are necessary and sufficient for the induction of zebrafish oocyte maturation. Besides cytoplasmic polyadenylation element-binding protein (CPEB) and cis-acting elements in the 3′ untranslated region (UTR), Pumilio1 and a cis-acting element in the coding region of cyclin B1 mRNA are important for ...
Tópico(s): Lipid metabolism and biosynthesis
2014 - Elsevier BV | Biochemical and Biophysical Research Communications
Tomohiro Naruse, Toshio Hattori, Haruo Miura, Kazuki Takahashi,
We have studied the thermal degradation of unidirectional carbon-FRP (CFRP) rings. First, we developed a method based on the ring tensile test for evaluating hoop directional strength. And we calculated the distribution of hoop stress by a finite-element method considering friction. Then, we measured the thermal reduction of fatigue strength of unidirectional CFRP rings. To evaluate the degradation of fatigue strength, we applied the Arrhenius equation to confirm the relation between temperature ...
Tópico(s): Mechanical Behavior of Composites
2001 - Elsevier BV | Composite Structures
Mana Katano, Kazuki Takahashi, Tomonari Hirano, Yusuke Kazama, Tomoko Abe, Hirokazu Tsukaya, Ali Ferjani,
Efficient use of seed nutrient reserves is crucial for germination and establishment of plant seedlings. Mobilizing seed oil reserves in Arabidopsis involves β-oxidation, the glyoxylate cycle, and gluconeogenesis, which provide essential energy and the carbon skeletons needed to sustain seedling growth until photoautotrophy is acquired. We demonstrated that H(+)-PPase activity is required for gluconeogenesis. Lack of H(+)-PPase in fugu5 mutants increases cytosolic pyrophosphate (PPi) levels, which ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2016 - Frontiers Media | Frontiers in Plant Science
Daiki Tamba, Osamu Kubo, Masaya Oda, Shun Osaka, Kazuki Takahashi, Hiroshi Tabata, Kentaro Kaneko, Shizυo Fujita, Mitsuhiro Katayama,
The surface structure of α-Ga2O3(0001) grown on an α-Al2O3(0001) substrate by mist chemical vapor deposition was studied by coaxial impact-collision ion scattering spectroscopy (CAICISS) and atomic force microscopy (AFM). The minimum step height observed in the AFM image was 0.21 ± 0.01 nm, coinciding with the height of three atomic layers of α-Ga2O3(0001). It was revealed by CAICISS analysis that the surface of α-Ga2O3(0001) is terminated by a Ga layer followed by an O layer, which is consistent with ...
Tópico(s): Semiconductor materials and devices
2016 - American Institute of Physics | Applied Physics Letters
Nobuyuki Sakurai, Kazuki Takahashi, Natsuko Emura, Takashi Fujii, Hiroki Hirayama, Soichi KAGEYAMA, Tsutomu HASHIZUME, Ken Sawai,
The functions of POU class 5 transcription factor 1 (Oct-4) and caudal-type homeobox 2 (Cdx2) in the differentiation of the murine inner cell mass (ICM) and trophectoderm (TE) have been described in detail. However, little is known about the roles of OCT-4 and CDX2 in preimplantation bovine embryos. To elucidate their functions during early development in bovine embryos, we performed OCT-4 and CDX2 downregulation using RNA interference. We injected OCT-4- or CDX2-specific short interfering RNAs (siRNAs) ...
Tópico(s): Animal Genetics and Reproduction
2016 - Mary Ann Liebert, Inc. | Cellular Reprogramming
Natsuko Emura, Nobuyuki Sakurai, Kazuki Takahashi, Tsutomu HASHIZUME, Ken Sawai,
Oct-4, a member of the POU family of transcription factors, is a key factor that regulates the segregation of the inner cell mass (ICM) and the trophectoderm (TE) during the transition from morula to blastocyst in mice. However, little is known about its role in porcine early embryogenesis. To determine the function of OCT-4 in the ICM and TE segregation of porcine embryos, we studied the developmental morphology of porcine embryos using RNA interference technology. Our experiments demonstrated that ...
Tópico(s): Renal and related cancers
2016 - Japanese Society of Animal Reproduction | Journal of Reproduction and Development
Nobuyuki Sakurai, Kazuki Takahashi, Natsuko Emura, Tsutomu HASHIZUME, Ken Sawai,
Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found ...
Tópico(s): Epigenetics and DNA Methylation
2016 - Japanese Society of Animal Reproduction | Journal of Reproduction and Development
Ayumu Yamashita, Tadayasu Dotani, H. Ezuka, M. Kawasaki, Kazuki Takahashi,
The performance of the charge coupled devices on-board the ASCA satellite has been traced for five years after the launch. We found the gradual increase of the dark current and the decrease of the charge transfer efficiency. These changes may be explained by charge traps due to the radiation damage. The nature of the radiation damage is investigated in detail using various methods.
Tópico(s): Silicon and Solar Cell Technologies
1999 - Elsevier BV | Nuclear Instruments and Methods in Physics Research Section A Accelerators Spectrometers Detectors and Associated Equipment
Takahiro Μatsumoto, Kazuki Takahashi, Sumire Kanayama, Yuka Nakano, Hiromi Imai, Masumi Kibi, Daisuke Imahori, Tomohiro Hasei, Tetsushi Watanabe,
Tópico(s): Plant biochemistry and biosynthesis
2017 - Springer Science+Business Media | Journal of Natural Medicines
Nhi Chung, Y. S. Bogliotti, Wei Ding, Marcela Vilariño, Kazuki Takahashi, James Chitwood, Richard M. Schultz, Pablo J. Ross,
The substantial epigenetic remodeling that occurs during early stages of mammalian embryonic development likely contributes to reprogramming the parental genomes from a differentiated to a totipotent state and activation of the embryonic genome. Trimethylation of lysine 27 of histone 3 (H3K27me3) is a repressive mark that undergoes global dynamic changes during preimplantation development of several species. To ascertain the role of H3K27me3 in bovine preimplantation development we perturbed the ...
Tópico(s): Cancer-related molecular mechanisms research
2017 - Landes Bioscience | Epigenetics
Rie Iida‐Norita, Yasuhiro Suzuki, Yutaka Furutani, Kazuki Takahashi, Yasuhiro Yoshimatsu, Katarzyna A. Podyma‐Inoue, Tetsuro Watabe, Yasufumi Sato,
Vasohibin‐2 ( VASH 2) is a homolog of VASH 1, an endothelium‐derived angiogenesis inhibitor. Vasohibin‐2 is mainly expressed in cancer cells, and has been implicated in the progression of cancer by inducing angiogenesis and tumor growth. Although VASH 2 has been recently reported to be involved in epithelial–mesenchymal transition ( EMT ), its precise roles are obscure. The aim of the present study was to clarify the role of VASH 2 in the EMT of cancer cells in relation to transforming growth factor‐β ( ...
Tópico(s): Hedgehog Signaling Pathway Studies
2017 - Wiley | Cancer Science
Ismail Hossain, Toshiaki Tsunogae, Yukiyasu Tsutsumi, Kazuki Takahashi,
The Paleoproterozoic (1.7 Ga) basement rocks from Maddhapara, Bangladesh show a large range of chemical variations (e.g. SiO2 = 50.7–74.7%) and include diorite, quartz diorite, monzodiorite, quartz monzonite and granite. The pluton overall displays metaluminous, calc-alkaline orogenic suite; mostly I-type suites formed within subduction-related magmatism. The observed major elements show general trends for fractional crystallization. Trace element contents also indicate the possibility of a fractionation ...
Tópico(s): earthquake and tectonic studies
2017 - Elsevier BV | Journal of Asian Earth Sciences
Yuji Mitani, Yutaro Oki, Yukari Fujimoto, Takumi Yamaguchi, K. Iwata, Yu Watanabe, Kazuki Takahashi, Kanji Yamada, Akira Ishikawa,
The prevention of pneumonia is an urgent issue among Japanese older adults. However, little has been reported on the relationship between a Functional Independence Measure (FIM) and the Geriatric Nutrition Risk Index (GNRI) for the prevention of pneumonia in patients in long-term care facilities in Japan. We aimed to clarify the relevance of FIM and GNRI for inpatients with and without pneumonia.We identified 233 patients who were hospitalized in our long-term nursing hospital from April 2012 to September ...
Tópico(s): Nutrition and Health in Aging
2017 - Wiley | Geriatrics and gerontology international/Geriatrics & gerontology international
Kazuki Takahashi, Ryousuke Morimoto, Hiromitsu Tabeta, Mariko Asaoka, Masanori Ishida, Masayoshi Maeshima, Hirokazu Tsukaya, Ali Ferjani,
To reveal the logic of size regulation in multicellular organisms, we have used Arabidopsis thaliana as a model organism and its leaves as a model organ. We discovered the existence of a compensatory system, whereby a decrease in leaf cell number often triggers unusual cell enlargement. However, despite the large number of compensation-exhibiting mutants analyzed to date, we have only a limited understanding of the detailed molecular mechanisms triggering the decrease in cell number and subsequent ...
Tópico(s): Plant Molecular Biology Research
2017 - Oxford University Press | Plant and Cell Physiology
Kazuki Takahashi, Toshiaki Tsunogae, M. Santosh, Yusuke Takamura, Yukiyasu Tsutsumi,
The Lützow-Holm Complex (LHC) of East Antarctica forms a part of the latest Neoproterozoic–Cambrian high-grade metamorphic segment of the East African–Antarctic Orogen. Here we present new petrological, geochemical, and zircon U–Pb geochronological data on meta-igneous rocks from four localities (Austhovde, Telen, Skallevikshalsen, and Skallen) in the LHC, and evaluate the regional Paleoproterozoic (ca. 1.8 Ga) arc magmatism in this terrane for the first time. The geochemical features reveal a volcanic- ...
Tópico(s): Geochemistry and Geologic Mapping
2017 - Elsevier BV | Journal of Asian Earth Sciences
Yusuke Okamoto, Watal M. Iwasaki, Kazuto Kugou, Kazuki Takahashi, Arisa Oda, Koichi Sato, Wataru Kobayashi, Hidehiko Kawai, Ryo Sakasai, Akifumi Takaori‐Kondo, Takashi Yamamoto, Masato T. Kanemaki, Masato Taoka, Toshiaki Isobe, Hitoshi Kurumizaka, Hideki Innan, Kunihiro Ohta, Masamichi Ishiai, Minoru Takata,
During mild replication stress provoked by low dose aphidicolin (APH) treatment, the key Fanconi anemia protein FANCD2 accumulates on common fragile sites, observed as sister foci, and protects genome stability. To gain further insights into FANCD2 function and its regulatory mechanisms, we examined the genome-wide chromatin localization of FANCD2 in this setting by ChIP-seq analysis. We found that FANCD2 mostly accumulates in the central regions of a set of large transcribed genes that were extensively ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2018 - Oxford University Press | Nucleic Acids Research
Hayato Okamoto, Yasuhiro Yoshimatsu, Taishi Tomizawa, Akiko Kunita, Rina Takayama, Teppei Morikawa, Daisuke Komura, Kazuki Takahashi, Tsukasa Oshima, Moegi Sato, Mao Komai, Katarzyna A. Podyma‐Inoue, Hiroaki Uchida, Hirofumi Hamada, Katsuhito Fujiu, Shumpei Ishikawa, Masashi Fukayama, Takeshi Fukuhara, Tetsuro Watabe,
Abstract Malignant melanoma is one of the untreatable cancers in which conventional therapeutic strategies, including chemotherapy, are hardly effective. Therefore, identification of novel therapeutic targets involved in melanoma progression is urgently needed for developing effective therapeutic methods. Overexpression of interleukin-13 receptor α2 (IL13Rα2) is observed in several cancer types including glioma and pancreatic cancer. Although IL13Rα2 is implicated in the progression of various types ...
Tópico(s): Melanoma and MAPK Pathways
2019 - Nature Portfolio | Scientific Reports
Hirofumi Fujii, K. Hara, Kohei Hayashi, H. Kakuno, Hideyo Kodama, K. Nagamine, Kotaro Sato, Shin-Hong Kim, Atsuto Suzuki, T. Sumiyoshi, Kazuki Takahashi, Fumihiko Takasaki, Shuji Tanaka, Satoru Yamashita,
Abstract We have investigated the status of the nuclear fuel assemblies in the Unit-1 reactor of the Fukushima Daiichi nuclear power plant by cosmic muon radiography. In this study, muon tracking detectors were placed outside the reactor building. We succeeded in identifying the inner structure of the reactor complex, such as the reactor containment vessel, pressure vessel, and other structures of the reactor building, through the concrete wall of the reactor building. We found that a large number ...
Tópico(s): Neutrino Physics Research
2020 - University of Oxford | Progress of Theoretical and Experimental Physics
Mariko Takeuchi, Hirokazu Kuwahara, Takumi Murakami, Kazuki Takahashi, Rei Kajitani, Atsushi Toyoda, Takehiko Itoh, Moriya Ohkuma, Yuichi Hongoh,
Abstract Several Trichonympha protist species in the termite gut have independently acquired Desulfovibrio ectosymbionts in apparently different stages of symbiosis. Here, we obtained the near-complete genome sequence of Desulfovibrio phylotype ZnDsv-02, which attaches to the surface of Trichonympha collaris cells, and compared it with a previously obtained genome sequence of ‘Candidatus Desulfovibrio trichonymphae’ phylotype Rs-N31, which is almost completely embedded in the cytoplasm of Trichonympha ...
Tópico(s): Plant and animal studies
2020 - Springer Nature | The ISME Journal
Tomohiro Ogawa, Kazuki Takahashi, Sanjog S. Nagarkar, Koji Ohara, You‐lee Hong, Yusuke Nishiyama, Satoshi Horike,
A proton-conducting coordination polymer glass derived from a protic ionic liquid works as a moldable solid electrolyte and the anhydrous fuel cell showed I – V performance of 0.15 W cm −2 at 120 °C.
Tópico(s): Conducting polymers and applications
2020 - Royal Society of Chemistry | Chemical Science
So Maezawa, Akihiko Sakashita, Masashi Yukawa, Xiaoting Chen, Kazuki Takahashi, Kris G. Alavattam, Ippo Nakata, Matthew T. Weirauch, Artem Barski, Satoshi H. Namekawa,
Owing to bursts in the expression of thousands of germline-specific genes, the testis has the most diverse and complex transcriptome of all organs. By analyzing the male germline of mice, we demonstrate that the genome-wide reorganization of super-enhancers (SEs) drives bursts in germline gene expression after the mitosis-to-meiosis transition. SE reorganization is regulated by two molecular events: the establishment of meiosis-specific SEs via A-MYB (MYBL1), a key transcription factor for germline ...
Tópico(s): Ubiquitin and proteasome pathways
2020 - Nature Portfolio | Nature Structural & Molecular Biology
Akinori Yamanaka, Kazuki Takahashi,
Data assimilation (DA) based on Bayes' theorem helps improve the accuracy of numerical models and simultaneously enables the estimation of unknown parameters used in the numerical model by combining simulation results with observational data. We applied the local ensemble transform Kalman filter (LETKF), a computationally efficient and accurate DA methodology, to a phase-field model of dendritic solidification in a binary alloy. We demonstrated the efficiency of LETKF through numerical experiments ( ...
Tópico(s): Aluminum Alloy Microstructure Properties
2020 - Elsevier BV | Materials Today Communications
Akihiko Sakashita, So Maezawa, Kazuki Takahashi, Kris G. Alavattam, Masashi Yukawa, Yueh‐Chiang Hu, Shohei Kojima, Nicholas F. Parrish, Artem Barski, Mihaela Pavličev, Satoshi H. Namekawa,
Gene regulation in the germline ensures the production of high-quality gametes, long-term maintenance of the species and speciation. Male germline transcriptomes undergo dynamic changes after the mitosis-to-meiosis transition and have been subject to evolutionary divergence among mammals. However, the mechanisms underlying germline regulatory divergence remain undetermined. Here, we show that endogenous retroviruses (ERVs) influence species-specific germline transcriptomes. After the mitosis-to-meiosis ...
Tópico(s): CRISPR and Genetic Engineering
2020 - Nature Portfolio | Nature Structural & Molecular Biology