Hyun Yong Jin, Hiroyo Oda, Maoyi Lai, Rebecca L. Skalsky, Kelly Bethel, Jovan Shepherd, Seung Goo Kang, Wen‐Hsien Liu, Mohsen Sabouri-Ghomi, Bryan R. Cullen, Klaus Rajewsky, Changchun Xiao,
... Search for more papers by this author Klaus Rajewsky Klaus Rajewsky Program of Cellular and Molecular Medicine, Children' ... Search for more papers by this author Klaus Rajewsky Klaus Rajewsky Program of Cellular and Molecular Medicine, Children's ...
Tópico(s): MicroRNA in disease regulation
2013 - Springer Nature | The EMBO Journal
... Search for more papers by this author Klaus Rajewsky Klaus Rajewsky Institute for Genetics, University of Cologne, D- ... Search for more papers by this author Klaus Rajewsky Klaus Rajewsky Institute for Genetics, University of Cologne, D-50931 ...
Tópico(s): T-cell and B-cell Immunology
1998 - Springer Nature | The EMBO Journal
Kathrin Geiger, Ulf Klein, Andreas Bräuninger, Stefan Berger, Korinna Leder, Klaus Rajewsky, Martin‐Leo Hansmann, Ralf Küppers,
... Main, GermanySearch for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Cologne, ... Main, GermanySearch for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Cologne, GermanySearch ...
Tópico(s): T-cell and B-cell Immunology
2000 - Wiley | European Journal of Immunology
Ulf Klein, Ralf Küppers, Klaus Rajewsky,
... Cologne, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics University of Cologne Weyertal ... Cologne, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics University of Cologne Weyertal 121 ...
Tópico(s): Immunodeficiency and Autoimmune Disorders
1995 - Wiley | Annals of the New York Academy of Sciences
Ralf Küppers, Klaus Rajewsky, Min Zhao, G De Vries Simons, R Laumann, R. Fischer, Martin‐Leo Hansmann,
... Cologne, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics Weyertal 121 University of ... Cologne, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics Weyertal 121 University of Cologne ...
Tópico(s): Galectins and Cancer Biology
1995 - Wiley | Annals of the New York Academy of Sciences
Shinsuke Taki, Markus Schmitt, David M. Tarlinton, Irmgard Förster, Klaus Rajewsky,
... 41, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics University of Cologne Weyertal ... 41, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics University of Cologne Weyertal 121, ...
Tópico(s): T-cell and B-cell Immunology
1992 - Wiley | Annals of the New York Academy of Sciences
Toshitada Takemori, Klaus Rajewsky,
... West GermanySearch for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Cologne, ... West GermanySearch for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Cologne, West ...
Tópico(s): Immune Cell Function and Interaction
1984 - Wiley | Immunological Reviews
Fred Sablitzky, Andreas Radbruch, Klaus Rajewsky,
... Germany.Search for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal ... Germany.Search for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal 121, ...
Tópico(s): Cell Adhesion Molecules Research
1982 - Wiley | Immunological Reviews
Garnett Kelsoe, Michael Reth, Klaus Rajewsky,
... Germany.Search for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal ... Germany.Search for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal 121, ...
Tópico(s): Transgenic Plants and Applications
1980 - Wiley | Immunological Reviews
Hua Gu, Irmgard Förster, Klaus Rajewsky,
... 41, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics University of Cologne Weyertal ... 41, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY Institute for Genetics University of Cologne Weyertal 121, ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1992 - Wiley | Annals of the New York Academy of Sciences
Thomas Christoph, Robert C. Rickert, Klaus Rajewsky,
... author on: Oxford Academic PubMed Google Scholar Klaus Rajewsky Klaus Rajewsky Institute for Genetics, University of CologneWeyertal 121, 50931 ...
1994 - Oxford University Press | International Immunology
Irmgard Förster, Paulo Vieira, Klaus Rajewsky,
... author on: Oxford Academic PubMed Google Scholar Klaus Rajewsky Klaus Rajewsky Institute for Genetics, University of CologneWeyertal 121, D- ...
Tópico(s): Immune Cell Function and Interaction
1989 - Oxford University Press | International Immunology
Gregory C. Ippolito, Jukka Pelkonen, Lars Nitschke, Klaus Rajewsky, Harry W. Schroeder,
... Würzburg, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY The Center for Blood Research, Harvard Medical ... Würzburg, GermanySearch for more papers by this authorKLAUS RAJEWSKY, KLAUS RAJEWSKY The Center for Blood Research, Harvard Medical School, ...
Tópico(s): Immune Cell Function and Interaction
2003 - Wiley | Annals of the New York Academy of Sciences
Dirk Löffert, Stefan Schaal, Andreas Ehlich, Richard R. Hardy, Yong‐Rui Zou, Werner Müller, Klaus Rajewsky,
... USA.Search for more papers by this authorKlaus Rajewsky, Corresponding Author Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal ... USA.Search for more papers by this authorKlaus Rajewsky, Corresponding Author Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal 121, ... Avenue, Philadelphia, PA 19111, USA.Address correspondence to Klaus Rajewsky, Institute for Genetics, University of Cologne, Weyertal 121. ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1994 - Wiley | Immunological Reviews
Verena Labi, Siying Peng, Filippos Klironomos, Mathias Munschauer, Nicolai Kastelic, Tirtha Chakraborty, Katia Schoeler, Emmanuel Derudder, Manuela Martella, Guido Mastrobuoni, Luis R. Hernández-Miranda, Ines Lahmann, Christine Kocks, Carmen Birchmeier, Stefan Kempa, Leticia Quintanilla‐Martínez, Markus Landthaler, Nikolaus Rajewsky, Klaus Rajewsky,
Knockout of the ubiquitously expressed miRNA-17∼92 cluster in mice produces a lethal developmental lung defect, skeletal abnormalities, and blocked B lymphopoiesis. A shared target of miR-17∼92 miRNAs is the pro-apoptotic protein BIM, central to life-death decisions in mammalian cells. To clarify the contribution of miR-17∼92:Bim interactions to the complex miR-17∼92 knockout phenotype, we used a system of conditional mutagenesis of the nine Bim 3′ UTR miR-17∼92 seed matches. Blocking miR-17∼92: ...
Tópico(s): RNA Interference and Gene Delivery
2019 - Cold Spring Harbor Laboratory Press | Genes & Development
Yitzhak Reizel, Ofra Sabag, Yael Skversky‐Blocq, Adam Spiro, Benjamin E. Steinberg, Diana N. Bernstein, Amber Wang, Julia E. Kieckhaefer, Catherine Li, Eli Pikarsky, Rena Levin-Klein, Alon Goren, Klaus Rajewsky, Klaus H. Kaestner, Howard Cedar,
Development in mammals is accompanied by specific de novo and demethylation events that are thought to stabilize differentiated cell phenotypes. We demonstrate that a large percentage of the tissue-specific methylation pattern is generated postnatally. Demethylation in the liver is observed in thousands of enhancer-like sequences associated with genes that undergo activation during the first few weeks of life. Using. conditional gene ablation strategy we show that the removal of these methyl groups ...
Tópico(s): RNA modifications and cancer
2018 - Nature Portfolio | Nature Communications
Sergei B. Koralov, Stefan A. Muljo, Gunther R. Galler, Azra Krek, Tirtha Chakraborty, Chryssa Kanellopoulou, Kari Jensen, Bradley S. Cobb, Matthias Merkenschlager, Nikolaus Rajewsky, Klaus Rajewsky,
To explore the role of Dicer-dependent control mechanisms in B lymphocyte development, we ablated this enzyme in early B cell progenitors. This resulted in a developmental block at the pro- to pre-B cell transition. Gene-expression profiling revealed a miR-17∼92 signature in the 3′UTRs of genes upregulated in Dicer-deficient pro-B cells; a top miR-17∼92 target, the proapoptotic molecule Bim, was highly upregulated. Accordingly, B cell development could be partially rescued by ablation of Bim or ...
Tópico(s): RNA Interference and Gene Delivery
2008 - Cell Press | Cell
Manfred Kraus, Marat Alimzhanov, Nikolaus Rajewsky, Klaus Rajewsky,
We previously showed that type I interferon-induced, Cre-mediated ablation of surface BCR expression in mature B cells through Ig-heavy chain deletion results in apoptosis of these cells. This led to the hypothesis that survival signals from the BCR are vital for mature B cells. Here, we test two critical assumptions of this model. First, we demonstrate loss of mature B cells upon induced mutation of a signaling module of the BCR, not precluding BCR surface expression. Second, we show that the cells ...
Tópico(s): NF-κB Signaling Pathways
2004 - Cell Press | Cell
Dmitry V. Kuprash, Marat Alimzhanov, Alexei V. Tumanov, Sergei I. Grivennikov, Alexander N. Shakhov, Ludmila N. Drutskaya, Michael W. Marino, Regina L. Turetskaya, Arthur O. Anderson, Klaus Rajewsky, Klaus Pfeffer, Sergei A. Nedospasov,
Homologous genes and gene products often have redundant physiological functions. Members of the tumor necrosis factor (TNF) family of cytokines can signal activation, proliferation, differentiation, costimulation, inhibition, or cell death, depending on the type and status of the target cell. TNF, lymphotoxin alpha (LTalpha), and LTbeta form a subfamily of a larger family of TNF-related ligands with their genes being linked within a compact 12-kb cluster inside the major histocompatibility complex ...
Tópico(s): Immune Cell Function and Interaction
2002 - Taylor & Francis | Molecular and Cellular Biology
Robert G. Endres, Marat Alimzhanov, Thomas Plitz, Agnes Fütterer, Marie Kosco‐Vilbois, Sergei A. Nedospasov, Klaus Rajewsky, Klaus Pfeffer,
The formation of germinal centers (GCs) represents a crucial step in the humoral immune response. Recent studies using gene-targeted mice have revealed that the cytokines tumor necrosis factor (TNF), lymphotoxin (LT) alpha, and LTbeta, as well as their receptors TNF receptor p55 (TNFRp55) and LTbetaR play essential roles in the development of GCs. To establish in which cell types expression of LTbetaR, LTbeta, and TNF is required for GC formation, LTbetaR-/-, LTbeta-/-, TNF-/-, B cell-deficient (BCR-/-), ...
Tópico(s): Immune Response and Inflammation
1999 - Rockefeller University Press | The Journal of Experimental Medicine
Ulrich Krawinkel, Matthias Cramer, Claudia Berek, Günter J. Hämmerling, Samuel J. Black, Klaus Rajewsky, Klaus Eichmann,
The antigen receptor of B lymphocytes is a classical antibody molecule whose specificity is determined by six hypervariable regions, three of which belong to the light chain and three to the heavy chain. The antigen receptor of T lymphocytes, on the other hand, is a molecule of thus far unknown structure that does not appear to be of the conventional immunoglobulin (Ig) design. In this paper, the problem of the molecular nature of the T-cell receptor is discussed on the basis of two lines of experimental ...
Tópico(s): Immune Cell Function and Interaction
1977 - Cold Spring Harbor Laboratory Press | Cold Spring Harbor Symposia on Quantitative Biology
Samuel J. Black, G J Hämmerling, Claudia Berek, Klaus Rajewsky, Klaus Eichmann,
Guinea pig anti-idiotypic antibodies (anti-Id) of the IgG1 class, directed to an A/J antibody to Group A streptococcal carbohydrate (A-CHO), or directed to a BALB/c myeloma protein that binds the same antigen, stimulate B-precursor cells as well as T-helper cells when injected into mice of the appropriate strain. The strain-specific induction of both precursor and helper activity was detected by in vitro secondary responses of primed spleen cells to A-CHO or to 2,4,6-trinitrophenyl (TNP) upon challenge ...
Tópico(s): vaccines and immunoinformatics approaches
1976 - Rockefeller University Press | The Journal of Experimental Medicine
Jingwei Zhang, Thomas Sommermann, Xun Li, Lutz Gieselmann, Kathrin de la Rosa, Maria Stecklum, Florian Klein, Christine Kocks, Klaus Rajewsky,
Introduction Epstein-Barr virus (EBV) infection in humans is associated with a wide range of diseases including malignancies of different origins, most prominently B cells. Several EBV latent genes are thought to act together in B cell immortalization, but a minimal set of EBV genes sufficient for transformation remains to be identified. Methods Here, we addressed this question by transducing human peripheral B cells from EBV-negative donors with retrovirus expressing the latent EBV genes encoding ...
Tópico(s): Parvovirus B19 Infection Studies
2023 - Frontiers Media | Frontiers in Immunology
Tomoharu Yasuda, T. Wirtz, Baochun Zhang, Thomas Wunderlich, Marc Schmidt‐Supprian, Thomas Sommermann, Klaus Rajewsky,
... Planck Institute for Biochemistry, 82152 Martinsried, Germany Correspondence: klaus.rajewsky{at}mdc-berlin.de and tomoharu.yasuda{at} ...
Tópico(s): Immune Cell Function and Interaction
2013 - Cold Spring Harbor Laboratory Press | Cold Spring Harbor Symposia on Quantitative Biology
Die Primärwirkung von Röntgenstrahlen einer Dosis von etwa 0,5 bis 150 Millionen r auf die kristallisierte Trockensubstanz von Aminosäuren und Peptiden wurde mit Hilfe chemischer, biochemischer und physikalisch-chemischer Arbeitsmethoden untersucht. Es wurde gefunden, daß in allen prinzipiell möglichen Fällen folgende Reaktionen stets wiederkehren : Aminbildung infolge Decarboxylierung; Bildung einer α-Imino- bzw. α-Ketocarbonsäure infolge einer Dehydrierung in α-,β-Stellung; Bildung von β,γ- bzw. ...
Tópico(s): Mass Spectrometry Techniques and Applications
1957 - De Gruyter | Zeitschrift für Naturforschung B
Changchun Xiao, Dinis Pedro Calado, Gunther R. Galler, To‐Ha Thai, Heide Christine Patterson, Jing Wang, Nikolaus Rajewsky, Timothy P. Bender, Klaus Rajewsky,
(Cell 131, 146–159; October 5, 2007) In Figure 2C of this article, we inadvertently used an incorrect FACS plot (duplication of the FACS plot representing mir150+/− mice) in the lower-right panel representing mir150−/− mice. The corrected panel is shown below. The small differences of the percentages in two of the panels on the right are due to an updated FlowJo software. We apologize for this error and any confusion it may have caused. The conclusions of the paper are not affected. MiR-150 Controls ...
Tópico(s): MicroRNA in disease regulation
2016 - Cell Press | Cell
Changchun Xiao, Dinis Pedro Calado, Gunther R. Galler, To‐Ha Thai, Heide Christine Patterson, Jing Wang, Nikolaus Rajewsky, Timothy P. Bender, Klaus Rajewsky,
Summary MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this ...
Tópico(s): Immune Cell Function and Interaction
2007 - Cell Press | Cell
Marat Alimzhanov, Dmitry V. Kuprash, Marie Kosco‐Vilbois, Arne Luz, Regina L. Turetskaya, Alexander Tarakhovsky, Klaus Rajewsky, Sergei A. Nedospasov, Klaus Pfeffer,
The tumor necrosis factor (TNF) family cytokines lymphotoxin (LT) α and LTβ form heterotrimers that are expressed on the surface of activated lymphocytes and natural killer cells; LTα homotrimers can be secreted as well. Mice with a disrupted LTα gene lack lymph nodes (LN), Peyer’s patches (PP), and follicular dendritic cell (FDC) networks and reveal profound defects of the splenic architecture. However, it is unclear which of these abnormalities is the result of the absence in LTα homotrimers or LTαβ ...
Tópico(s): T-cell and B-cell Immunology
1997 - National Academy of Sciences | Proceedings of the National Academy of Sciences
To‐Ha Thai, Dinis Pedro Calado, Stefano Casola, K. Mark Ansel, Changchun Xiao, Yingzi Xue, Andrew Murphy, David Frendewey, David M. Valenzuela, Jeffery L. Kutok, Marc Schmidt‐Supprian, Nikolaus Rajewsky, George D. Yancopoulos, Anjana Rao, Klaus Rajewsky,
MicroRNAs are small RNA species involved in biological control at multiple levels. Using genetic deletion and transgenic approaches, we show that the evolutionarily conserved microRNA-155 (miR-155) has an important role in the mammalian immune system, specifically in regulating T helper cell differentiation and the germinal center reaction to produce an optimal T cell-dependent antibody response. miR-155 exerts this control, at least in part, by regulating cytokine production. These results also ...
Tópico(s): Circular RNAs in diseases
2007 - American Association for the Advancement of Science | Science
Silvia Monticelli, K. Mark Ansel, Changchun Xiao, Nicholas D. Socci, Anna M. Krichevsky, To‐Ha Thai, Nikolaus Rajewsky, Debora S. Marks, Chris Sander, Klaus Rajewsky, Anjana Rao, Kenneth S. Kosik,
MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. However, in mammals, expression data are principally based on whole tissue analysis and are still very incomplete.We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. Complementary oligonucleotides capable of hybridizing ...
Tópico(s): Cancer-related molecular mechanisms research
2005 - BioMed Central | Genome biology