Eveline R. Goethals, Sarah S. Jaser, C.M. Verhaak, Sofie Prikken, Kristina Casteels, Koen Luyckx, Alan M. Delamater,
AIMS Although research exists on parental communication in adolescents with type 1 diabetes (T1D), the role of communication by health care providers remains understudied. Grounded in Self-Determination Theory, this study examined the role of autonomy-supportive communication (i.e., providing meaningful rationale and offering choices with regard to treatment recommendations) by providers and parents, and how they interact in the prediction of diabetes outcomes. Methods In this cross-sectional study, ...
Tópico(s): Childhood Cancer Survivors' Quality of Life
2020 - Elsevier BV | Diabetes Research and Clinical Practice
Eveline R. Goethals, Bart Soenens, Maartje de Wit, Maarten Vansteenkiste, Lori M. Laffel, Kristina Casteels, Koen Luyckx,
Objective Grounded in Self-Determination Theory, this study examines the role of parental expectations and communication style (ie, in an autonomy-supportive vs controlling way) in the prediction of adolescent motivation (ie, internalization or defiance) to adhere to self-management for type 1 diabetes. Methods Structural Equation Modeling was used in a cross-sectional, multi-informant study of 129 adolescents (Mage = 14.43; 54.4% girls), 110 mothers, and 98 fathers. Adolescents reported on self-motivation, ...
Tópico(s): Child and Adolescent Psychosocial and Emotional Development
2019 - Wiley | Pediatric Diabetes
Bart de Laat, Akila Weerasekera, Gil Leurquin‐Sterk, Willy Gsell, Guy Bormans, Uwe Himmelreich, Cindy Casteels, Koen Van Laere,
Abstract To study the role of the glutamatergic system in alcohol abuse disorders, we conducted a longitudinal study of alcohol exposure in rats. Rats were followed with 1 H‐magnetic resonance spectroscopy during a baseline, exposure and abstinence phase to measure prefrontal glutamate and glycine concentrations. Additionally, 18 F‐FPEB PET was used to assess metabotropic glutamate receptor 5 (mGluR5) availability before and after exposure to alcohol or saccharin. Finally, individual anxiety levels ...
Tópico(s): Receptor Mechanisms and Signaling
2018 - Wiley | Addiction Biology
Bart de Laat, Akila Weerasekera, Gil Leurquin‐Sterk, Guy Bormans, Uwe Himmelreich, Cindy Casteels, Koen Van Laere,
Cocaine addiction is a disorder that still lacks diagnostic biomarkers or effective pharmacotherapy. We present findings on a rat model of cocaine self-administration that was followed up longitudinally using the metabotropic glutamate receptor type 5 (mGluR5) tracer 18 F-3-fluoro-5-[(pyridin-3-yl)ethynyl]benzonitrile ( 18 F-FPEB) PET, proton MR spectroscopy ( 1 H-MRS), and behavioral tests. Methods: Forty-two Wistar rats were scanned with 18 F-FPEB PET and 1 H-MRS before and after sucrose or intravenous ...
Tópico(s): Advanced MRI Techniques and Applications
2018 - Society of Nuclear Medicine and Molecular Imaging | Journal of Nuclear Medicine
Eveline R. Goethals, Leen Oris, Bart Soenens, Cynthia A. Berg, Sofie Prikken, Nady Van Broeck, Ilse Weets, Kristina Casteels, Koen Luyckx,
To examine the role of diabetes-specific parental regulation and general parenting dimensions (responsiveness and psychological control) in treatment adherence throughout adolescence and emerging adulthood.A total of 521 patients (aged 14-25 years) with Type 1 diabetes, 407 mothers, and 345 fathers were included. Analyses within and across informants examined the associations between the parenting variables and treatment adherence (and potential moderation effects in these associations).Lower psychological ...
Tópico(s): Childhood Cancer Survivors' Quality of Life
2017 - Oxford University Press | Journal of Pediatric Psychology
Eveline R. Goethals, Maartje de Wit, Nady Van Broeck, Jurgen Lemiere, Dagmar Van Liefferinge, Susanne Böhler, Marian De wulf, Elke Dello, Jolien Laridaen, Lynn Van Hecke, Shana Van Impe, Kristina Casteels, Koen Luyckx,
Objective Managing type 1 diabetes (T1D) requires the ability to make complex and critical decisions regarding treatment, to execute complex tasks accurately, and to make adjustments when problems arise. This requires effective neuropsychological competences of patients and their families, especially in the domain of executive functioning (EF): the ability to self-monitor, plan, solve problems, and set priorities. Previous research focused mainly on child EF, neglecting the impact of parental EF. ...
Tópico(s): Bipolar Disorder and Treatment
2017 - Wiley | Pediatric Diabetes
Melissa Crabbé, Nina Dirkx, Cindy Casteels, Koen Van Laere,
Abstract Metabotropic glutamate receptors (mGluRs) have been proposed as promising therapeutic targets to correct the dysregulated glutamate signaling, associated with neurodegenerative pathologies. Of all mGluR subtypes, especially mGluR5 acts as a modulator of glutamate-induced excitotoxicity. To study the behavior of mGluR5 following localized excitotoxicity, we utilised a pharmacological model that portrays exacerbated neuronal glutamate release, mediated by the endogenous excitotoxin quinolinic ...
Tópico(s): Neuroscience and Neural Engineering
2019 - Nature Portfolio | Scientific Reports
Melissa Crabbé, Anke Van der Perren, Savannah Kounelis, Thomas Lavreys, Guy Bormans, Veerle Baekelandt, Cindy Casteels, Koen Van Laere,
Rat models based on viral vector-mediated overexpression of α-synuclein are regarded as highly valuable models that closely mimic cardinal features of human Parkinson's disease (PD) such as L-DOPA-dependent motor impairment, dopaminergic neurodegeneration and α-synuclein inclusions. To date, the downstream effects of dopaminergic cell loss on brain glucose metabolism, including the neuroinflammation component, have not been phenotyped in detail for this model. Cerebral glucose metabolism was monitored ...
Tópico(s): Nerve injury and regeneration
2019 - Elsevier BV | Experimental Neurology
Anke Van der Perren, Cindy Casteels, Koen Van Laere, Rik Gijsbers, Chris Van den Haute, Veerle Baekelandt,
In order to study the molecular pathways of Parkinson's disease (PD) and to develop novel therapeutic strategies, scientific investigators rely on animal models. The identification of PD-associated genes has led to the development of genetic PD models. Most transgenic α-SYN mouse models develop gradual α-SYN pathology but fail to display clear dopaminergic cell loss and dopamine-dependent behavioral deficits. This hurdle was overcome by direct targeting of the substantia nigra with viral vectors ...
Tópico(s): Autism Spectrum Disorder Research
2016 - MyJOVE | Journal of Visualized Experiments
Melissa Crabbé, Anke Van der Perren, Ilse Bollaerts, Savannah Kounelis, Veerle Baekelandt, Guy Bormans, Cindy Casteels, Lieve Moons, Koen Van Laere,
The purinergic P2X7 receptor is a key mediator in (neuro)inflammation, a process that is associated with neurodegeneration and excitotoxicity in Parkinson's disease (PD). Recently, P2X7 imaging has become possible with [11C]JNJ-(54173)717. We investigated P2X7 availability, in comparison with availability of the translocator protein (TSPO), in two well-characterized rat models of PD using in vitro autoradiography at multiple time points throughout the disease progression. Rats received either a unilateral ...
Tópico(s): Neurological Complications and Syndromes
2019 - Frontiers Media | Frontiers in Neuroscience
Cindy Casteels, Guy Bormans, Koen Van Laere,
Small animal PET can be applied to study molecular processes in animal models of a variety of human diseases. In order to keep the animals in a restricted position during imaging, anaesthesia is in many instances inevitable. Using small animal PET and ex vivo autoradiography, we examined the influence of pentobarbital and isoflurane anaesthesia on the rat brain uptake of [18F]MK-9470, a radioligand for the type 1 cannabinoid receptor. PET imaging was performed on adult Wistar rats under pentobarbital ( ...
Tópico(s): Forensic Toxicology and Drug Analysis
2010 - Springer Science+Business Media | European Journal of Nuclear Medicine and Molecular Imaging
Esther Wolfs, Bryan Holvoet, Laura Ordovás, Natacha Breuls, Nicky Helsen, Matthias Schönberger, Susanna Raitano, Tom Struys, B Vanbilloen, Cindy Casteels, Maurilio Sampaolesi, Koen Van Laere, Ivo Lambrichts, Catherine M. Verfaillie, Christophe M. Deroose,
Molecular imaging is indispensable for determining the fate and persistence of engrafted stem cells. Standard strategies for transgene induction involve the use of viral vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes. Methods: We used zinc finger nucleases to induce stable expression of human imaging reporter genes into the safe-harbor locus adeno-associated virus integration site 1 in human embryonic stem cells. Plasmids were generated carrying reporter genes ...
Tópico(s): Virus-based gene therapy research
2017 - Society of Nuclear Medicine and Molecular Imaging | Journal of Nuclear Medicine
Anke Van der Perren, Cindy Casteels, Koen Van Laere, Rik Gijsbers, Chris Van den Haute, Veerle Baekelandt,
In order to study the molecular pathways of Parkinson's disease (PD) and to develop novel therapeutic strategies, scientific investigators rely on animal models. The identification of PD-associated genes has led to the development of genetic PD models. Most transgenic α-SYN mouse models develop gradual α-SYN pathology but fail to display clear dopaminergic cell loss and dopamine-dependent behavioral deficits. This hurdle was overcome by direct targeting of the substantia nigra with viral vectors ...
Tópico(s): Autism Spectrum Disorder Research
2016 - MyJOVE | Journal of Visualized Experiments
De Laat Bart, Gsell Willy, Kristof Govaerts, Uwe Himmelreich, Cindy Casteels, Van Laere Koen,
... Laat Willy Gsell Kristof Govaerts Uwe Himmelreich Cindy Casteels Koen Van Laere Google Bart De Laat Willy Gsell Kristof Govaerts Uwe Himmelreich Cindy Casteels Koen Van Laere Google Scholar Bart De Laat Willy Gsell Kristof Govaerts Uwe Himmelreich Cindy Casteels Koen Van Laere PubMed Bart De Laat Willy Gsell Kristof Govaerts Uwe Himmelreich Cindy Casteels Koen Van Laere Related Article in Frontiers Google Scholar ...
Tópico(s): Neural dynamics and brain function
2016 - Frontiers Media | Frontiers in Aging Neuroscience
Tom Struys, Kristof Govaerts, Wouter Oosterlinck, Cindy Casteels, Annelies Bronckaers, Michel Koole, Koen Van Laere, Paul Herijgers, Ivo Lambrichts, Uwe Himmelreich, Tom Dresselaers,
We have characterized both acute and long-term vascular and metabolic effects of unilateral common carotid artery occlusion in mice by in vivo magnetic resonance imaging and positron emission tomography. This common carotid artery occlusion model induces chronic cerebral hypoperfusion and is therefore relevant to both preclinical stroke studies, where it serves as a control condition for a commonly used mouse model of ischemic stroke, and neurodegeneration, as chronic hypoperfusion is causative ...
Tópico(s): Biochemical effects in animals
2016 - SAGE Publishing | Journal of Cerebral Blood Flow & Metabolism
Dieter Ory, Anna M. Planas, Tom Dresselaers, Willy Gsell, Andrey Postnov, Sofie Celen, Cindy Casteels, Uwe Himmelreich, Zeger Debyser, Koen Van Laere, Alfons Verbruggen, Guy Bormans,
The goal of this study was to measure functional and structural aspects of local neuroinflammation induced by intracerebral injection of lipopolysaccharide (LPS) in rats using TSPO microPET imaging with [18 F]DPA-714, magnetic resonance imaging (MRI), in vitro autoradiography and immunohistochemistry (IHC) in order to characterize a small animal model for screening of new PET tracers targeting neuroinflammation. Rats were injected stereotactically with LPS (50 μg) in the right striatum and with saline ...
Tópico(s): S100 Proteins and Annexins
2015 - Elsevier BV | Nuclear Medicine and Biology
Jenny Ceccarini, Rawaha Ahmad, Laura Van de Vliet, Cindy Casteels, Mathieu Vandenbulcke, Wim Vandenberghe, Koen Van Laere,
Many Huntington disease (HD) mutation carriers already have cognitive and psychiatric symptoms in the premanifest (premotor) phase of the disease (pre-HD), but the molecular underpinnings of these symptoms are not well understood. Previous work has shown reduced availability of the cerebral type 1 cannabinoid receptor (CB1R) in manifest HD. Here, we investigated whether CB1R binding is related to cognitive and psychiatric symptoms in pre-HD mutation carriers. Methods: CB1R binding was measured with ...
Tópico(s): Cannabis and Cannabinoid Research
2018 - Society of Nuclear Medicine and Molecular Imaging | Journal of Nuclear Medicine
Evy Cleeren, Cindy Casteels, Karolien Goffin, Michel Koole, Koen Van Laere, Peter Janssen, Wim Van Paesschen,
Summary Objective We investigated changes in the endocannabinoid system and glucose metabolism during temporal lobe epileptogenesis. Methods Because it is rarely possible to study epileptogenesis in humans, we applied the electrical amygdala kindling model in nonhuman primates to image longitudinal changes in type 1 cannabinoid receptor ( CB 1R) binding and cerebral glucose metabolism. Two rhesus monkeys received [ 18 F]‐ MK ‐9470 and fluorodeoxyglucose–positron emission tomography ([ 18 F]‐ FDG ‐PET) scans ...
Tópico(s): Epilepsy research and treatment
2018 - Wiley | Epilepsia
Jenny Ceccarini, Cindy Casteels, Michel Koole, Guy Bormans, Koen Van Laere,
Recent biochemical and post-mortem evidence suggests involvement of the endocannabinoid system in alcohol drinking behaviour and dependence. Using [18F]MK-9470 small-animal PET imaging, our primary objective was to evaluate in vivo type 1 cannabinoid receptor (CB1R) binding changes in rats subjected to several ethanol conditions: (1) at baseline, (2) after acute intraperitoneal administration of ethanol (4 g/kg) or saline, (3) after 7 days of forced chronic ethanol consumption, and (4) after abstinence ...
Tópico(s): Neuroscience and Neuropharmacology Research
2013 - Springer Science+Business Media | European Journal of Nuclear Medicine and Molecular Imaging
Caroline Vandeputte, Jean‐Marc Taymans, Cindy Casteels, Frea Coun, Yicheng Ni, Koen Van Laere, Veerle Baekelandt,
Abstract Background Accurate and reproducible behavioral tests in animal models are of major importance in the development and evaluation of new therapies for central nervous system disease. In this study we investigated for the first time gait parameters of rat models for Parkinson's disease (PD), Huntington's disease (HD) and stroke using the Catwalk method, a novel automated gait analysis test. Static and dynamic gait parameters were measured in all animal models, and these data were compared to ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2010 - BioMed Central | BMC Neuroscience
Koen Veys, Zheng Fan, Moheb Ghobrial, Ann Bouché, Melissa García‐Caballero, Kim Vriens, Nadine V. Conchinha, Aline Seuwen, Felix Schlegel, Tatiane Gorski, Melissa Crabbé, Paola Gilardoni, Raphaela Ardicoglu, Johanna Schaffenrath, Cindy Casteels, Gino De Smet, Ilse Smolders, Koen Van Laere, E. Dale Abel, Sarah‐Maria Fendt, Aileen Schroeter, Joanna Kalucka, Anna Rita Cantelmo, Thomas Wälchli, Annika Keller, Peter Carmeliet, Katrien De Bock,
Endothelial cells (ECs) are highly glycolytic and generate the majority of their energy via the breakdown of glucose to lactate. At the same time, a main role of ECs is to allow the transport of glucose to the surrounding tissues. GLUT1 (glucose transporter isoform 1/Slc2a1) is highly expressed in ECs of the central nervous system (CNS) and is often implicated in blood-brain barrier (BBB) dysfunction, but whether and how GLUT1 controls EC metabolism and function is poorly understood.We evaluated the ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2020 - Lippincott Williams & Wilkins | Circulation Research
Melissa Crabbé, Anke Van der Perren, Akila Weerasekera, Uwe Himmelreich, Veerle Baekelandt, Koen Van Laere, Cindy Casteels,
Several lines of evidence point to alterations in glutamatergic signaling in Parkinson's disease (PD) and levodopa-induced dyskinesia (LID), involving the metabotropic glutamate receptor type 5 (mGluR5). Using small-animal positron emission tomography (PET) with [18F]FPEB and proton magnetic resonance spectroscopy, we investigated cerebral changes in the mGluR5 and glutamate/glutamine availability in vivo in PD rats and following onset of LIDs. In parallel, behavioral tests were performed. Comparing ...
Tópico(s): Parkinson's Disease Mechanisms and Treatments
2017 - Elsevier BV | Neurobiology of Aging
Bart de Laat, Gil Leurquin‐Sterk, Sofie Celen, Guy Bormans, Michel Koole, Koen Van Laere, Cindy Casteels,
The metabotropic glutamate receptor 5 (mGluR5) is a high-interest target for PET imaging because it plays a role in several pathologies, including addiction, schizophrenia, and fragile X syndrome. Methods: We studied the pharmacokinetics of 18 F-FPEB (3- 18 F-fluoro-5-(2-pyridinylethynyl)benzonitrile), a selective PET radioligand for mGluR5, and used it to quantify mGluR5 in rat brain. Quantification was performed using both arterial sampling in combination with compartment models and simplified reference ...
Tópico(s): Receptor Mechanisms and Signaling
2015 - Society of Nuclear Medicine and Molecular Imaging | Journal of Nuclear Medicine
Maarten Ooms, Roma Rietjens, Janaki Raman Rangarajan, Kathleen Vunckx, Sara Valdeolivas, Frederik Maes, Uwe Himmelreich, Javier Fernández‐Ruíz, Guy Bormans, Koen Van Laere, Cindy Casteels,
Several lines of evidence imply early alterations in endocannabinoid and phosphodiesterase 10A (PDE10A) signaling in Huntington disease (HD). Using [18F]MK-9470 and [18F]JNJ42259152 small-animal positron emission tomography (PET), we investigated for the first time cerebral changes in type 1 cannabinoid (CB1) receptor binding and PDE10A levels in vivo in presymptomatic, early symptomatic, and late symptomatic HD (R6/2) mice, in relation to glucose metabolism ([18F]FDG PET), brain morphology (magnetic resonance ...
Tópico(s): Neurological disorders and treatments
2014 - Elsevier BV | Neurobiology of Aging
Jenny Ceccarini, Titia Hompes, Anne Verhaeghen, Cindy Casteels, Hendrik Peuskens, Guy Bormans, Stephan Claes, Koen Van Laere,
Involvement of the type 1 cannabinoid receptor (CB1R) in the effects of alcohol on the brain is supported by animal experiments, but how in vivo CB1R levels are altered in alcoholic patients is still unclear. To assess the short-time effects of a binge drinking episode on CB1R availability, 20 healthy social drinkers underwent [(18)F]MK-9470-positron emission tomography (PET) at baseline and after intravenous ethanol administration (ALC ACU). Moreover, 26 alcoholic patients underwent sequential CB1R ...
Tópico(s): Alcohol Consumption and Health Effects
2014 - Society for Neuroscience | Journal of Neuroscience
Cindy Casteels, Michel Koole, Sofie Celen, Guy Bormans, Koen Van Laere,
[18F]MK-9470 is an inverse agonist for the type 1 cannabinoid (CB1) receptor allowing its use in PET imaging. We characterized the kinetics of [18F]MK-9470 and evaluated its ability to quantify CB1 receptor availability in the rat brain. Dynamic small-animal PET scans with [18F]MK-9470 were performed in Wistar rats on a FOCUS-220 system for up to 10 h. Both plasma and perfused brain homogenates were analysed using HPLC to quantify radiometabolites. Displacement and blocking experiments were done using ...
Tópico(s): Forensic Toxicology and Drug Analysis
2012 - Springer Science+Business Media | European Journal of Nuclear Medicine and Molecular Imaging
Koen Van Laere, Cindy Casteels, Sophie Lunskens, Karolien Goffin, Igor D. Grachev, Guy Bormans, Wim Vandenberghe,
The type 1 cannabinoid receptor (CB1) is a crucial modulator of synaptic transmission in brain and has been proposed as a potential therapeutic target in Parkinson's disease (PD), especially for treatment of levodopa-induced dyskinesias (LID). Our aim was to measure CB1 levels in brains of PD patients in vivo and to investigate the relation between CB1 availability and LID. We studied 12 healthy controls and 29 PD patients (9 drug-naïve patients with early PD, 10 patients with advanced PD and LID, and ...
Tópico(s): Neuroscience and Neuropharmacology Research
2011 - Elsevier BV | Neurobiology of Aging
Koen Van Laere, Cindy Casteels, I. Dhollander, Karolien Goffin, Igor D. Grachev, Guy Bormans, Wim Vandenberghe,
The type 1 cannabinoid receptor (CB1) is a crucial modulator of synaptic transmission in the brain. Animal and postmortem human data suggest that mutant huntingtin represses CB1 transcription. Our aim was to measure CB1 levels in the brains of Huntington disease (HD) patients in vivo. Methods: Twenty symptomatic HD patients and 14 healthy controls underwent PET with the novel CB1 ligand N -[2-(3-cyano-phenyl)-3-(4-(2- 18 F-fluorethoxy)phenyl)-1-methylpropyl]-2-(5-methyl-2-pyridyloxy)-2-methylproponamide. ...
Tópico(s): Neuroscience and Neuropharmacology Research
2010 - Society of Nuclear Medicine and Molecular Imaging | Journal of Nuclear Medicine
Kris van Kuyck, Nathalie Gérard, Koen Van Laere, Cindy Casteels, Guido Pieters, Loes Gabriëls, Bart Nuttin,
Tópico(s): Transcranial Magnetic Stimulation Studies
2009 - Elsevier BV | Journal of Psychiatric Research
Cindy Casteels, Erwin Lauwers, Guy Bormans, Veerle Baekelandt, Koen Van Laere,
The unilateral 6-hydroxydopamine (6-OHDA) lesion rat model is a well-known acute model for Parkinson's disease (PD). Its validity has been supported by invasive histology, behavioral studies and electrophysiology. Here, we have characterized this model in vivo by multitracer imaging [glucose metabolism and dopamine transporter (DAT)] in relation to behavioral and histological parameters. Eighteen female adult Wistar rats (eight 6-OHDA-lesioned, ten controls) were investigated using multitracer [18F]-fluoro- ...
Tópico(s): Neurological disorders and treatments
2007 - Springer Science+Business Media | European Journal of Nuclear Medicine and Molecular Imaging