Toshio Iwasaki, Asako Kounosu, Miho Aoshima, Daijiro Ohmori, Takeo Imai, Akio Urushiyama, Nathaniel J. Cosper, Robert A. Scott,
... 266Crossref PubMed Google Scholar, 13Iwasaki T. Aoshima M. Kounosu A. Oshima T. Ghisla S. Kroneck P. Macheroux ... sequenced, and initially characterized (13Iwasaki T. Aoshima M. Kounosu A. Oshima T. Ghisla S. Kroneck P. Macheroux ... 4S] cluster binding motifs (13Iwasaki T. Aoshima M. Kounosu A. Oshima T. Ghisla S. Kroneck P. Macheroux ... Scopus (36) Google Scholar, 13Iwasaki T. Aoshima M. Kounosu A. Oshima T. Ghisla S. Kroneck P. Macheroux ... 4Fe-4S]-[4Fe-4S] (13Iwasaki T. Aoshima M. Kounosu A. Oshima T. Ghisla S. Kroneck P. Macheroux ...
Tópico(s): Photosynthetic Processes and Mechanisms
2002 - Elsevier BV | Journal of Biological Chemistry
Asako Kounosu, Zhongrui Li, Nathaniel J. Cosper, Jacob E. Shokes, Robert A. Scott, Takeo Imai, Akio Urushiyama, Toshio Iwasaki,
... Google Scholar, 27Cosper N.J. Eby D.M. Kounosu A. Kurosawa N. Neidle E.L. Kurtz Jr., ... number AB047031 (27Cosper N.J. Eby D.M. Kounosu A. Kurosawa N. Neidle E.L. Kurtz Jr., ... linkage (DDBJ accession number AB023295) 2T. Iwasaki, A. Kounosu, H. Iwasaki, T. Imai, A. Urushiyama, Y. Hayashi- ... A. Scott, manuscript in preparation. 2T. Iwasaki, A. Kounosu, H. Iwasaki, T. Imai, A. Urushiyama, Y. Hayashi- ... PDF PubMed Scopus (31) Google Scholar, 30Iwasaki T. Kounosu A. Dikanov S.A. Kawamori A. Yamauchi J. ... ferredoxin (ARF) (27Cosper N.J. Eby D.M. Kounosu A. Kurosawa N. Neidle E.L. Kurtz Jr., ...
Tópico(s): Porphyrin Metabolism and Disorders
2004 - Elsevier BV | Journal of Biological Chemistry
Toshinobu Horii, Hiroki Ohtsuka, Mari Osaki, Hisashi Ohkuni,
... Scholar H. Ohtsuka, H. Ohtsuka Medca Japan Laboratory, Kounosu, Saitama, Japan Search for other works by this ... Scholar H. Ohkuni H. Ohkuni Medca Japan Laboratory, Kounosu, Saitama, Japan Search for other works by this ...
Tópico(s): Herpesvirus Infections and Treatments
2009 - Oxford University Press | Letters in Applied Microbiology
Albert L. Whetstone, S. Kounosu,
A tunnel diode coincidence circuit which is versatile and simple is described. It is intended to serve as the elemental block for building up complicated fast logic schemes which are frequently required in high energy physics experiments. Performance results are given as well as discussions of numerous possible variations on the design.
Tópico(s): Semiconductor Quantum Structures and Devices
1962 - American Institute of Physics | Review of Scientific Instruments
Junzo SHIMOIIZAKA, K. Nakatsuka, Toyohisa Fujita, Akira Kounosu,
The development of sink-float separators for materials of different densities, which utilize the levitation force of magnetic fluid, have been recently reported. The system usually consists of an electromagnet, separation cell, equipment for recovery of magnetic fluid smeared on the separated products, and controlling system. This paper describes and discusses the construction of sink-float separators using permanent magnets and provides test results for artificial mixtures of nonferrous metals. ...
Tópico(s): Minerals Flotation and Separation Techniques
1980 - IEEE Magnetics Society | IEEE Transactions on Magnetics
Toshio Iwasaki, Rimma I. Samoilova, Asako Kounosu, Daijiro Ohmori, Sergei A. Dikanov,
CW EPR spectra of reduced [2Fe−2S](Cys)3(His)1 clusters of mammalian mitoNEET soluble domain appear to produce features resulting from the interaction of the electron spins of the two adjacent clusters, which can be explained by employing the local spin model. This model favors the reduction of the outermost iron with His87 and Cys83 ligands, which is supported by orientation-selected hyperfine sublevel correlation (HYSCORE) characterization of the uniformly 15N-labeled mitoNEET showing one strongly ...
Tópico(s): Metal complexes synthesis and properties
2009 - American Chemical Society | Journal of the American Chemical Society
Nathaniel J. Cosper, D. Matthew Eby, Asako Kounosu, Norio Kurosawa, Ellen L. Neidle, Donald M. Kurtz, Toshio Iwasaki, Robert A. Scott,
Proteins containing Rieske-type [2Fe-2S] clusters play important roles in many biological electron transfer reactions. Typically, [2Fe-2S] clusters are not directly involved in the catalytic transformation of substrate, but rather supply electrons to the active site. We report herein X-ray absorption spectroscopic (XAS) data that directly demonstrate an average increase in the iron-histidine bond length of at least 0.1 A upon reduction of two distantly related Rieske-type clusters in archaeal Rieske ...
Tópico(s): Porphyrin Metabolism and Disorders
2002 - Wiley | Protein Science
Toru Mizukami, William I. Chang, Igor Garkavtsev, Nancy Kaplan, D. Rachel Lombardi, Tomohiro Matsumoto, Osami Niwa, Asako Kounosu, Mitsuhiro Yanagida, Thomas G. Marr, David Beach,
We present the application of a nonrandom sequencetagged site (STS) content detection method in mapping an entire genome, that of fission yeast. The novelty of our strategy is in the use of STS probes made from both ends of cosmid clones, selected on the basis of “sample without replacement” (only library clones that show no previous positive hybridization are selected and made into probes). We developed powerful techniques, based on consistency analysis, for error detection and contig assembly. In ...
Tópico(s): Genomics and Phylogenetic Studies
1993 - Cell Press | Cell
Noboru KUBOTA, Minoru Kounosu, Kyoichi Saito, Kazuyuki Sugita, Kohei Watanabe, Takanobu Sugo,
A phenyl group was appended to the epoxy-group-containing polymer chain grafted onto the pore surface of a porous hollow-fiber membrane for hydrophobic interaction chromatography. For introduction of the phenyl group at a density sufficient for protein collection, glycidyl methacrylate (GMA) grafted membrane was immersed in 1 M phenol aqueous solution whose pH ranged from 9 to 13. A bovine serum albumin (BSA) buffer solution containing 2 M (NH4)2SO4 was forced to permeate through the resultant porous ...
Tópico(s): Advanced NMR Techniques and Applications
1996 - Elsevier BV | Reactive and Functional Polymers
Toshio Iwasaki, Asako Kounosu, Taketoshi Uzawa, Rimma I. Samoilova, Sergei A. Dikanov,
The weakly coupled 15N atoms around a reduced Rieske [2Fe−2S] cluster of the uniformly 15N-labeled, hyperthermostable archaeal Rieske protein appear to produce readily observable cross-peaks in the HYSCORE spectra, with the well-resolved couplings of 0.3−0.4 MHz for the Nε and 1.1 MHz for the peptide backbone nitrogens, in addition to the contributions from the coordinated Nδ atoms. These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly ...
Tópico(s): Ammonia Synthesis and Nitrogen Reduction
2004 - American Chemical Society | Journal of the American Chemical Society
Toshio Iwasaki, Asako Kounosu, Derrick R.J. Kolling, Antony R. Crofts, Sergei A. Dikanov, Akihisa Jin, Takeo Imai, Akio Urushiyama,
The pH-dependent resonance Raman (RR) spectral changes of the cytochrome bc1-associated, high-potential Rieske proteins have frequently been invoked to explain the redox-linked ionization behavior. We report herein RR spectral data of archaeal and bacterial Rieske proteins that directly demonstrate the pH-dependent changes near and above pKa,ox2, but not around pKa,ox1, of the visible circular dichroism (CD) transitions. The RR spectral changes are attributed to modification of the immediate [2Fe−2S] ...
Tópico(s): Enzyme Structure and Function
2004 - American Chemical Society | Journal of the American Chemical Society
Sergei A. Dikanov, A. A. Shubin, Asako Kounosu, Toshio Iwasaki, Rimma I. Samoilova,
Tópico(s): Metal Extraction and Bioleaching
2004 - Springer Science+Business Media | JBIC Journal of Biological Inorganic Chemistry
Recent studies have indicated that post‐translational flavinylation of succinate dehydrogenase subunit A (SdhA) in eukaryotes and bacteria require the chaperone‐like proteins Sdh5 and SdhE, respectively. How does covalent flavinylation occur in prokaryotes, which lack SdhE homologs? In this study, I showed that covalent flavinylation in two hyperthermophilic bacteria/archaea lacking SdhE, Thermus thermophilus and Sulfolobus tokodaii , requires heat and dicarboxylic acid. These thermophilic bacteria/ ...
Tópico(s): Biochemical and Molecular Research
2014 - Wiley | FEBS Letters
Shunsuke Watanabe, Yuka Kounosu, Hiroshi Shimada, Atsushi Sakamoto,
Although generally recognized for its role in nitrogen recycling and remobilization, purine catabolism might also be involved in the plant response and adaptation to environmental stress. Previously, we demonstrated that allantoin, a major purine intermediary metabolite in stressed plants, stimulates abscisic acid production and activates stress-responsive gene expression, leading to increased tolerance to abiotic stress in Arabidopsis seedlings. Here, we show that dysfunction of purine degradation, ...
Tópico(s): Plant Stress Responses and Tolerance
2014 - Japanese Society for Plant Biotechnology | Plant Biotechnology
Noboru KUBOTA, Minoru Kounosu, Kyoichi Saito, Kazuyuki Sugita, Kohei Watanabe, Takanobu Sugo,
A novel porous membrane was prepared by radiation-induced graft polymerization of an epoxy-group-containing vinyl monomer, glycidyl methacrylate, and subsequent addition of phenol and water. A phenyl group was appended to the polymer chain grafted on to a porous polyethylene hollow-fibre membrane. The presence of a diol group together with the phenyl group was required to reduce non-selective adsorption of the protein. A bovine serum albumin (BSA) phosphate buffer solution containing 2 M ammonium ...
Tópico(s): Radioactive element chemistry and processing
1995 - Elsevier BV | Journal of Chromatography A
Noboru KUBOTA, Minoru Kounosu, Kyoichi Saito, Kazuyuki Sugita, Kohei Watanabe, Takanobu Sugo,
A porous hollow-fiber membrane containing a phenyl group as a hydrophobic ligand was prepared by radiation-induced graft polymerization of glycidyl methacrylate, followed by successive ring-opening reactions with phenol and water. Bovine serum albumin (BSA) was bound to the ligand during permeation of a BSA solution in phosphate buffer containing 2M (NH4)2SO4 through the pores of the hollow fiber. Subsequent elution with an (NH4)2SO4-free buffer exhibited an elution percentage of 82%. Repeated cycles ...
Tópico(s): Advanced Drug Delivery Systems
1997 - Elsevier BV | Journal of Membrane Science
N. Kubota, Minoru Kounosu, Kazuki Saito, Kazuyuki Sugita, Kohei Watanabe, Takanobu Sugo,
Phenyl and butyl groups as hydrophobic ligands for protein binding were appended to the poly(glycidyl methacrylate) chain grafted onto a pore surface of a porous polyethylene hollow-fiber membrane with a pore size of 0.2 μm. A hydrophobic ligand density of the modified membranes from 0.5 to 2.5 mmol/g was obtained, while pure water flux of the hollow fibers was 80% that of the original hollow fiber. Favorable kinetics, wherein an increasing permeation rate provides an increasing binding rate of ...
Tópico(s): Polymer Surface Interaction Studies
1997 - American Chemical Society | Biotechnology Progress
Mayu Hikone, Naoya Sakamoto, Masayuki Ota, Takuya Washino, Kenichiro Kobayashi, Sentaro Iwabuchi, Haruko Kazama, Akiko Kounosu, Kumiko Negishi, Yusuke Ainoda, Shigekazu Iguchi, Atsushi Yoshida, Ken Kikuchi, Kenji Ohnishi,
Gemella is a facultative anaerobic Gram-positive coccus and a rare cause of infective endocarditis (IE). Gram staining may eventually misidentify the organism, which tends to easily decolorize and manifest as either Gram-negative or Gram-variable. Commercial biochemical tests are often used to identify Gemella, but the methods they employ sometimes lack accuracy. A 52-year-old woman was diagnosed with Gemella taiwanensis IE after initial identification of the pathogen as Gemella haemolysans using ...
Tópico(s): Streptococcal Infections and Treatments
2017 - Elsevier BV | Journal of Infection and Chemotherapy
Tanzila Afrin, Kazunori Murase, Asuka Kounosu, Vicky L. Hunt, Mark W. Bligh, Yasunobu Maeda, Akina Hino, Haruhiko Maruyama, Isheng Jason Tsai, Taisei Kikuchi,
Soil-transmitted helminths (STHs) are medically important parasites that infect 1.5 billion humans globally, causing a substantial disease burden. These parasites infect the gastrointestinal tract of their host where they co-exist and interact with the host gut bacterial flora, leading to the coevolution of the parasites, microbiota and host organisms. However, little is known about how these interactions change through time with the progression of infection. Strongyloidiasis is a human parasitic ...
Tópico(s): Parasites and Host Interactions
2019 - Frontiers Media | Frontiers in Cellular and Infection Microbiology
Asuka Kounosu, Kazunori Murase, Akemi Yoshida, Haruhiko Maruyama, Taisei Kikuchi,
Abstract The development and application of next-generation sequencing (NGS) have enabled comprehensive analyses of the microbial community through extensive parallel sequencing. Current analyses of the eukaryotic microbial community are primarily based on polymerase chain reaction amplification of 18S rRNA gene (rDNA) fragments. We found that widely-used 18S rDNA primers can amplify numerous stretches of the bacterial 16S rRNA gene, preventing the high-throughput detection of rare eukaryotic species, ...
Tópico(s): Microbial Community Ecology and Physiology
2019 - Nature Portfolio | Scientific Reports
Toshio Iwasaki, Asako Kounosu, Rimma I. Samoilova, Sergei A. Dikanov,
The hyperfine couplings for strongly and weakly coupled 15N nuclei around a reduced Rieske [2Fe−2S] center of uniformly 15N-labeled, hyperthermostable archaeal Rieske protein at pH 13.3 were determined by hyperfine sublevel correlation (HYSCORE) spectroscopy and compared with those at physiological pH. Significant changes in the hyperfine couplings of the terminal histidine Nδ ligands and Nε nuclei were observed between them, which can be explained by not only the redistribution of the unpaired electron ...
Tópico(s): Metalloenzymes and iron-sulfur proteins
2006 - American Chemical Society | Journal of the American Chemical Society
A. Toshimitsu Yokobori, Atsushi Kamei, S. Kounosu,
Real materials inevitably contain both stress concentrators as cracks or notches and as dislocations, that is, both macroscopic and microscopic one. From this viewpoint, a concept of combined micro and macro fracture mechanics is proposed for low-stress brittle fracture of steels.
Tópico(s): Rock Mechanics and Modeling
1974 - Springer Science+Business Media | International Journal of Fracture
Yan Yu, Hiroshi Wada, Jun‐ichi Inoue, Shinji Imaizumi, Yuichi Kounosu, Kazuma Tsuboi, Hidetoshi Matsumoto, Minoru Ashizawa, Takehiko Mori, Mie Minagawa, Akihiko Tanioka,
An unzipped graphene oxide nanoribbon is patterned by surface selective deposition or inkjet printing, and its successive reduction to a graphene nanoribbon by vacuum annealing leads to highly conducting transparent graphene nanoribbon films. Such graphene nanoribbon films are used as source and drain electrodes in bottom-contact organic transistors based on pentacene and sexithiophene as well as C60.
Tópico(s): Organic Electronics and Photovoltaics
2011 - Institute of Physics | Applied Physics Express
Toshio Iwasaki, Asako Kounosu, Ye Tao, Zhongrui Li, Jacob E. Shokes, Nathaniel J. Cosper, Takeo Imai, Akio Urushiyama, Robert A. Scott,
Proteins containing Rieske-type [2Fe-2S] clusters play essential functions in all three domains of life. We engineered the two histidine ligands to the Rieske-type [2Fe-2S] cluster in the hyperthermophilic archaeal Rieske-type ferredoxin from Sulfolobus solfataricus to modify types and spacing of ligands and successfully converted the metal and cluster type at the redox-active site with a minimal structural change to a native Rieske-type protein scaffold. Spectroscopic analyses unambiguously established ...
Tópico(s): CO2 Reduction Techniques and Catalysts
2005 - Elsevier BV | Journal of Biological Chemistry
Taisei Kikuchi, Mehmet Dayı, Vicky L. Hunt, Kenji Ishiwata, Atsushi Toyoda, Asuka Kounosu, Simo Sun, Yasunobu Maeda, Yoko Kondo, Belkisyolé Alarcón de Noya, Óscar Noya, Somei Kojima, Toshiaki Kuramochi, Haruhiko Maruyama,
Abstract The cryptic parasite Sparganum proliferum proliferates in humans and invades tissues and organs. Only scattered cases have been reported, but S. proliferum infection is always fatal. However, S. proliferum ’s phylogeny and life cycle remain enigmatic. To investigate the phylogenetic relationships between S. proliferum and other cestode species, and to examine the mechanisms underlying pathogenicity, we sequenced the entire genomes of S. proliferum and a closely related non–life-threatening ...
Tópico(s): Helminth infection and control
2021 - Nature Portfolio | Communications Biology
Takutoshi Sugiyama, Madoka Ichikawa‐Seki, Hironobu Sato, Asuka Kounosu, Mio Tanaka, Haruhiko Maruyama,
Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis in Japan. Quality characteristics of the test were accessed by receiver operating characteristic (ROC) analysis, with sera from fasciolosis patients (n = 10), patients with no evidence of parasitic infections (n = 29), and patients with other helminth infections (n = 119). Both the sensitivity and specificity of the test achieved 100% with the control samples. To ...
Tópico(s): Parasite Biology and Host Interactions
2021 - Elsevier BV | Parasitology International
Takayoshi Iseki, Kazuo Yamaguchi, Hideo TAGAI,
... Hideo Tagai, Takayoshi Iseki, Takeo Saeki, and Toshiro Kounosu, “Solid State Reactions Between CaO and a Single ...
Tópico(s): Luminescence Properties of Advanced Materials
1970 - Wiley | Journal of the American Ceramic Society
Toshio Iwasaki, Asako Kounosu, Derrick R.J. Kolling, Sangmoon Lhee, Antony R. Crofts, Sergei A. Dikanov, Takuro Uchiyama, Takashi Kumasaka, Hiroyuki Ishikawa, Miwa Kono, Takeo Imai, Akio Urushiyama,
The rate of quinol oxidation by cytochrome bc(1)/b(6)f complex is in part associated with the redox potential (E(m)) of its Rieske [2Fe-2S] center, for which an approximate correlation with the number of hydrogen bonds to the cluster has been proposed. Here we report comparative resonance Raman (RR) characterization of bacterial and archaeal high-potential Rieske proteins and their site-directed variants with a modified hydrogen bond network around the cluster. Major differences among their RR spectra ...
Tópico(s): Enzyme Structure and Function
2006 - Wiley | Protein Science
... Hideo Tagai, Takayoshi Iseki, Takeo Saeki, and Toshiro Kounosu, “Solid State Reactions Between CaO and a Single ...
Tópico(s): Recycled Aggregate Concrete Performance
1970 - Wiley | Journal of the American Ceramic Society
Hideo TAGAI, Takayoshi Iseki, Takeo Saeki, Toshiro Kounosu,
Reflection microscopy, electron probe X-ray microanalysis and single crystal X-ray diffraction were used to analyse the reaction between CaO and a single crystal of Al2O3 at high temperatures. A single crystal of α-Al2O3 was embedded in CaO powder and heated in the temperature range 1200° to 1500°C. The polished cross sections of the specimens were investigated by the methods above mentioned. The following results were obtained.1) Calcium aluminate layer appeared on the surface of a single crystal ...
Tópico(s): Aluminum Alloys Composites Properties
1969 - Ceramic Society of Japan | Journal of the Ceramic Association Japan