Jakub Bogusławski, Grzegorz Soboń, Karol Tarnowski, Rafał Zybała, Krzysztof Mars, Andrzej Mikuła, Krzysztof M. Abramski, Jarosław Sotor,
We report on the generation of 152-nJ Q-switched pulses in all-polarization-maintaining Er-doped fiber laser. The laser is passively modulated by the antimony telluride (Sb2Te3) layer sputtered on a surface of the side-polished fiber in order to exploit the evanescent field interaction in optically nonlinear material. The laser cavity is designed in an extremely simple way comprising, in addition to fibers, only one component and the saturable absorber, forming a robust, compact, and stable source ...
Tópico(s): Laser-Matter Interactions and Applications
2016 - SPIE | Optical Engineering
Jakub Bogusławski, Grzegorz Soboń, Rafał Zybała, Krzysztof Mars, Andrzej Mikuła, Krzysztof M. Abramski, Jarosław Sotor,
We report a study on a hybrid mode-locked fiber laser with two saturable absorbers: slow and fast, integrated in a single device. Amorphous antimony telluride (Sb(2)Te(3)) layer was deposited on side-polished fiber to form the slow saturable absorber due to the third order nonlinear susceptibility of Sb(2)Te(3). Additionally, an unsymmetrical design of the device causes polarization-dependent losses and together with polarization controller allows to use a nonlinear polarization evolution to form ...
Tópico(s): Photonic Crystal and Fiber Optics
2015 - Optica Publishing Group | Optics Express
Michał Łach, Kinga Pławecka, Joanna Marczyk, Celina Ziejewska, Maria Hebdowska-Krupa, Marek Nykiel, Marek Hebda, Krzysztof Miernik, Dariusz Mierzwiński, Kinga Korniejenko, Janusz Mikuła, Krzysztof Smoroń,
Diatomites are one of the most interesting materials found in various regions of the world. In Poland, there is the only active diatomite mine in Jawornik Ruski in the Podkarpacie region. Diatomite fields in Poland with an average SiO2 content of 72% are found in the Carpathian Mountains, in the Leszczawka region. Geological data indicate the existence of at least 4 large deposits of this material, but one deposit (Jawornik) is being exploited. The diatomite from the Polish deposit differs from other ...
Tópico(s): Adsorption, diffusion, and thermodynamic properties of materials
2023 - Elsevier BV | Journal of Cleaner Production
Ewa Drożdż, Agnieszka Łącz, Andrzej Koleżyński, Andrzej Mikuła, Krzysztof Mars,
A series of highly porous Sr1-xYxTiO3 samples (with x = 0, 2, 4, 6 and 8 mol%) were prepared by sol-gel method and sintered at 1200 °C. Single phase materials were obtained for up to 6 mol% of yttrium admixture as confirmed by XRD measurements. For samples with 8 mol% of dopant, traces of Y2Ti2O7 phase were found indicating yttrium solubility limit in this structures at below 8 mol%. For all samples electrochemical impedance spectroscopy measurements were carried out in air and hydrogen (reduced) atmosphere. ...
Tópico(s): Advancements in Solid Oxide Fuel Cells
2016 - Elsevier BV | Solid State Ionics
Paweł Nieroda, Juliusz Leszczyński, Andrzej Mikuła, Krzysztof Mars, Mirosław J. Kruszewski, Andrzej Koleżyński,
Copper(I) sulphide is one of the most intensely studied superionic thermoelectric materials. High mobility of copper ions have positive impact on thermoelectric figure of merit, however it brings some stability drawbacks. In this work we compare thermoelectric properties of Cu2S synthesized under various conditions. All of the obtained powders were densified by induction hot pressing (IHP) at the same conditions. Phase composition, microstructure and thermoelectric homogeneity were investigated by ...
Tópico(s): Physics of Superconductivity and Magnetism
2020 - Elsevier BV | Ceramics International
Ewa Pruszyńska‐Oszmałek, Paweł Kołodziejski, P. Kaczmarek, Maciej Sassek, Dawid Szczepankiewicz, Robert Mikuła, Krzysztof W. Nowak,
It is well known that orexins are involved in the metabolism and endocrine function of rodent adipocytes, but there are no data on other animal species, including pigs. Therefore, in this study, we tested the hypothesis that orexin A (OxA) and orexin B (OxB) modulate the metabolism and endocrine functions of isolated porcine adipocytes and adipose tissue explants. Moreover, we characterized the possible mechanism of OxA action in porcine adipocytes. According to the results, both orexin receptor 1 ...
Tópico(s): Sleep and related disorders
2018 - Elsevier BV | Domestic Animal Endocrinology
Ewa E. Hennig, Michał Mikula, Krzysztof Goryca, Agnieszka Paziewska, Joanna K. Ledwon, Monika Nesteruk, Marek Woszczyński, Bożena Walewska‐Zielecka, Kazimiera Pyśniak, Jerzy Ostrowski,
Abstract One of the main questions regarding nonalcoholic fatty liver disease is the molecular background of the transition from simple steatosis ( SS ) to the inflammatory and fibrogenic condition of steatohepatitis ( NASH ). We examined the gene expression changes during progression from histologically normal liver to SS and NASH in models of obesity caused by hyperphagia or a high‐fat diet. Microarray‐based analysis revealed that the expression of 1445 and 264 probe sets was changed exclusively in SS ...
Tópico(s): Metabolism, Diabetes, and Cancer
2014 - Wiley | Journal of Cellular and Molecular Medicine
Natalia Maryan, Małgorzata Statkiewicz, Michał Mikula, Krzysztof Goryca, Agnieszka Paziewska, Adriana Strzałkowska, Michalina Dąbrowska, Mateusz Bujko, Jerzy Ostrowski,
Altered epigenetic mechanisms, similar to gene mutations, contribute to the pathogenesis and molecular heterogeneity of neoplasms, including colorectal cancer (CRC). Enhancer of zeste 2 (EZH2) is a histone methyltransferase, which is involved in epigenetic gene silencing and is aberrantly expressed in CRC. Therefore, the identification of the genes regulated by EZH2 in CRC is important to improve current understanding of its role in cancer epigenetics. The present study used chromatin immunoprecipitation ( ...
Tópico(s): Epigenetics and DNA Methylation
2012 - Spandidos Publishing | Molecular Medicine Reports
Barbara Górka, Joanna Skubis-Zegadło, Michał Mikula, Krzysztof Bardadin, Ewa Paliczka, Barbara Czarnocka,
NrCAM (neuron-glia-related cell-adhesion molecule) is primarily, although not solely, expressed in the nervous system. In the present study, NrCAM expression was analysed in a series (46) of papillary thyroid carcinomas (PTCs) and paired normal tissues (NT). Quantitative reverse transcriptase (QRT)-PCR revealed that NrCAM expression was upregulated in all PTCs compared to normal thyroid, whatever the stage or size of the primary tumour. NrCAM transcript levels were 1.3- to 30.7-fold higher in PTCs than ...
Tópico(s): S100 Proteins and Annexins
2007 - Springer Nature | British Journal of Cancer
Joanna Skubis-Zegadło, Anna Nikodemska, Ewa Przytuła, Michał Mikula, Krzysztof Bardadin, Jerzy Ostrowski, B. E. Wenzel, Barbara Czarnocka,
The Pendred syndrome gene (PDS) encodes a transmembrane protein, pendrin, which is expressed in follicular thyroid cells and participates in the apical iodide transport. Pendrin expression has been studied in various thyroid neoplasms by means of immunohistochemistry (IHC), Western blot and RT–quantitative real-time PCR. The expression was related to the functional activity of the thyroid tissue. Follicular cells of normal, nodular goitre and Graves' disease tissues express pendrin at the apical pole ...
Tópico(s): Thyroid Disorders and Treatments
2005 - Springer Nature | British Journal of Cancer
Artur A. Antoniewicz, Agnieszka Paziewska, Michał Mikula, Krzysztof Goryca, Michalina Dąbrowska, Sławomir Poletajew, Andrzej Borówka, Jerzy Ostrowski,
Aggressive intervention against the bladder wall during transurethral resection of bladder tumors (TURBT) causes damage and leakage from blood vessels to the bladder lumen. The aim of this study was to determine whether TURBT could increase the level of circulating urothelial cells. Expression of tumor markers, discriminative for nucleated blood cells and urothelium, was evaluated by quantitative (q) RT-PCR on RNA isolated from peripheral blood samples of 51 patients who underwent TURBT for ≥cT1c bladder ...
Tópico(s): Cancer Cells and Metastasis
2011 - Springer Science+Business Media | International Urology and Nephrology
Patrycja Sosińska, Justyna Mikuła‐Pietrasik, Krzysztof Książek,
The complexity of eukaryotic organisms involves the regulation of gene expression through DNA–protein, RNA–DNA, RNA–RNA, and RNA–protein interactions. The role of RNA molecules in the regulation of genes in higher species has become even more evident with the discovery that about 97% of transcription products are represented by non-protein coding RNAs (ncRNAs) including short ncRNAs and long ncRNAs (lncRNAs). In addition to the well-characterized role of ncRNAs in different physiological cellular ...
Tópico(s): RNA Research and Splicing
2015 - Elsevier BV | Mutation Research/Reviews in Mutation Research
Paweł Uruski, Agnieszka Sepetowska, Corinna Konieczna, Martyna Pakuła, Michał Wyrwa, Akylbek Tussupkaliyev, Andrzej Tykarski, Justyna Mikuła‐Pietrasik, Krzysztof Książek,
Various types of normal and cancer cells undergo senescence in response to carboplatin and paclitaxel, which are considered the gold standard treatments in ovarian cancer management. Surprisingly, the effect of these drugs on ovarian cancer cell senescence remained unknown.The experiments were conducted on primary high-grade serous ovarian cancer cells. Molecular markers of senescence were evaluated using cytochemistry and immunofluorescence. Cell cycle distribution was analyzed using flow cytometry. ...
Tópico(s): Mechanisms of cancer metastasis
2021 - BioMed Central | Cellular & Molecular Biology Letters
Martyna Pakuła, Ewa Mały, Paweł Uruski, Anna Witucka, Małgorzata Bogucka, Natalia Jaroszewska, Nicoletta Makowska, Arkadiusz Niklas, Rafał Moszyński, Stefan Sajdak, Andrzej Tykarski, Justyna Mikuła‐Pietrasik, Krzysztof Książek,
Spontaneous senescence of cancer cells remains a puzzling and poorly understood phenomenon. Here we comprehensively characterize this process in primary epithelial ovarian cancer cells (pEOCs). Analysis of tumors from ovarian cancer patients showed an abundance of senescent cells in vivo. Further, serially passaged pEOCs become senescent after a few divisions. These senescent cultures display trace proliferation, high expression of senescence biomarkers (SA--Gal, -H2A.X), growth-arrest in the G1 phase, ...
Tópico(s): Virus-based gene therapy research
2020 - Multidisciplinary Digital Publishing Institute | Cancers
Martyna Pakuła, Anna Witucka, Paweł Uruski, Artur Radziemski, Rafał Moszyński, Dariusz Szpurek, Konstantin Maksin, Aldona Woźniak, Stefan Sajdak, Andrzej Tykarski, Justyna Mikuła‐Pietrasik, Krzysztof Książek,
Mechanisms of transmesothelial invasion of ovarian cancer are still poorly understood. Here we examined whether this phenomenon may be determined by an expression of intercellular junctions in peritoneal mesothelial cells (PMCs). Analysis of ovarian tumors showed that cancer cells are localized below an intact layer of PMCs. The PMCs located near the invaded cancer cells displayed low expression of connexin 43, E-cadherin, occludin, and desmoglein, as well as expressed SA-β-Gal, a marker of senescence. ...
Tópico(s): Telomeres, Telomerase, and Senescence
2019 - Nature Portfolio | Scientific Reports
Małgorzata Kucińska, H. Piotrowska, Michał W. Łuczak, Justyna Mikuła‐Pietrasik, Krzysztof Książek, Milena Wozniak, Marcin Wierzchowski, Jarosław Dudka, Walter Jäger, Marek Murias,
Resveratrol and its higher hydroxylated analogs have been reported to possess a variety of biological properties including antioxidant as well as prooxidant effects. The antioxidant properties are assumed to enable these compounds to protect cells from oxidative damage, however prooxidant activity are held likely to be responsible for their cytotoxic or pro-apoptotic effects. In present study the effects of resveratrol (Res) and its three derivatives: 3,3′,4,4′-tetrahydroxy-trans-stilbene (M6), 3, ...
Tópico(s): Bioactive natural compounds
2014 - Elsevier BV | Chemico-Biological Interactions
Andrzej Mikuła, Krzysztof Mars, Paweł Nieroda, Paweł Rutkowski,
For the first time, an alternative way of improving the stability of Cu-based thermoelectric materials is proposed, with the investigation of two different copper chalcogenide–copper tetrahedrite composites, rich in sulfur and selenium anions, respectively. Based on the preliminary DFT results, which indicate the instability of Sb-doped copper chalcogenide, the Cu1.97S–Cu12Sb4S13 and Cu2−xSe–Cu3SbSe3 composites are obtained using melt-solidification techniques, with the tetrahedrite phase concentration ...
Tópico(s): Quantum Dots Synthesis And Properties
2021 - Multidisciplinary Digital Publishing Institute | Materials
Martyna Pakuła, Paweł Uruski, Arkadiusz Niklas, Aldona Woźniak, Dariusz Szpurek, Andrzej Tykarski, Justyna Mikuła‐Pietrasik, Krzysztof Książek,
The study was designed to establish whether high aggressiveness of high-grade serous ovarian cancer cells (HGSOCs), which display rapid growth, advanced stage at diagnosis and the highest mortality among all epithelial ovarian cancer histotypes, may be linked with a specific pattern of mesothelial-mesenchymal transition (MMT) elicited by these cells in normal peritoneal mesothelial cells (PMCs). Experiments were performed on primary PMCs, stable and primary ovarian cancer cells, tumors from patients ...
Tópico(s): Genetic factors in colorectal cancer
2019 - Multidisciplinary Digital Publishing Institute | Cancers
Michał Mikula, Magdalena Skrzypczak, Krzysztof Goryca, Katarzyna Paczkowska, Joanna K. Ledwon, Małgorzata Statkiewicz, Maria Kulecka, Marta Grzelak, Michalina Dąbrowska, Urszula Kuklinska, Jakub Karczmarski, Izabela Rumieńczyk, Kamil Jastrzębski, Marta Miączyńska, Krzysztof Ginalski, Karol Bomsztyk, Jerzy Ostrowski,
Genome-wide mechanisms that coordinate expression of subsets of functionally related genes are largely unknown. Recent studies show that receptor tyrosine kinases and components of signal transduction cascades including the extracellular signal-regulated protein kinase (ERK), once thought to act predominantly in the vicinity of plasma membrane and in the cytoplasm, can be recruited to chromatin encompassing transcribed genes. Genome-wide distribution of these transducers and their relationship to ...
Tópico(s): RNA regulation and disease
2016 - Oxford University Press | Nucleic Acids Research
Tomasz Rzymski, Michał Mikula, Katarzyna Wiklik, Krzysztof Brzózka,
Cyclin-dependent kinase (CDK) inhibitors have been developed as potential anticancer therapeutics and several nonselective compounds are currently in advanced clinical trials. This review is focused on the key biological roles of CDK8 kinase, which provide a proof-of-principle for continued efforts toward effective cancer treatment, targeting activity of this CDK family member. Among currently identified kinase inhibitors, several displayed significant selectivity for CDK8 and notably the effectiveness ...
Tópico(s): Advanced Breast Cancer Therapies
2015 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
Grzegorz Izydorczyk, Katarzyna Mikula, Dawid Skrzypczak, Krzysztof Trzaska, Κωνσταντίνος Μουστάκας, Anna Witek‐Krowiak, Katarzyna Chojnacka,
This literature review outlines the most important-agricultural and non-agricultural-types of sewage sludge management. The potential of waste sludge protein hydrolysates obtained by chemical sludge conditioning was reported. The discussed areas include acidic and alkaline hydrolysis, lime conditioning, polyelectrolyte dewatering and other supporting techniques such as ultrasounds, microwave or thermal methods. The legislative aspects related to the indication of the development method and admission ...
Tópico(s): Anaerobic Digestion and Biogas Production
2021 - Springer Science+Business Media | Environmental Science and Pollution Research
Andrzej Mikuła, Paweł Nieroda, Krzysztof Mars, Juliusz Dąbrowa, Andrzej Koleżyński,
A multi-phase composite based on the copper sulfide and bornite phases was obtained. The sintering process (inductive hot pressing method) and multiple thermoelectric measurements lead, however, to complete reconstruction of the structure. Structural, thermoelectric and stability studies indicate that the final material initially consisting of 90% Cu2S and 10% Cu5FeS4 is characterized by a thermoelectric figure of merit (ZT) of about 1.0 (900 [K]) with significantly improved stability in comparison ...
Tópico(s): Magnetic and transport properties of perovskites and related materials
2020 - Elsevier BV | Solid State Ionics
Juliusz Dąbrowa, Mirosław Stygar, Andrzej Mikuła, A. Knapik, Krzysztof Mroczka, Waldemar Tejchman, Marek Danielewski, Manfred Martin,
A high-entropy (Co,Cr,Fe,Mn,Ni)3O4 oxide, characterized by the Fd-3m single-phase, spinel structure, was synthesized for the first time. The material was based on the combination of Co, Cr, Fe, Mn and Ni elements, well known from the other group of high-entropy materials, namely high-entropy alloys. The microstructure of the material was studied using SEM + EDS and XRD methods, showing single-phase structure. Additional structural characterization was performed with use of Raman spectroscopy, also ...
Tópico(s): Advanced materials and composites
2017 - Elsevier BV | Materials Letters
Michał Mikula, Karol Bomsztyk, Krzysztof Goryca, Krzysztof Chojnowski, Jerzy Ostrowski,
The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a nucleic acid-binding protein that acts as a docking platform integrating signal transduction pathways to nucleic acid-related processes. Given that hnRNPK could be involved in other steps that compose gene expression the definition of its genome-wide occupancy is important to better understand its role in transcription and co-transcriptional processes. Here, we used chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) to analyze ...
Tópico(s): RNA and protein synthesis mechanisms
2013 - Elsevier BV | Journal of Biological Chemistry
Justyna Mikuła‐Pietrasik, Szymon Rutecki, Krzysztof Książek,
Tópico(s): Cancer Cells and Metastasis
2022 - Springer Nature | Cellular and Molecular Life Sciences
Justyna Mikuła‐Pietrasik, Paweł Uruski, Krzysztof Aniukiewicz, Patrycja Sosińska, Zbigniew Krasiński, Andrzej Tykarski, Krzysztof Książek,
Although the role of endothelium in varicose vein development is indisputable, the effect of the pathology on biological properties of endothelial cells remains unclear. Here we examined if the presence of varicose veins affects senescence of endothelial cells (HUVECs) and, if so, what will be the local and systemic outcome of this effect. Experiments showed that HUVECs subjected to serum from varicose patients display improved proliferation, increased expression of senescence marker, SA-β-Gal, and ...
Tópico(s): Dermatologic Treatments and Research
2016 - Hindawi Publishing Corporation | Oxidative Medicine and Cellular Longevity
Justyna Mikuła‐Pietrasik, Patrycja Sosińska, Krzysztof Książek,
Objective Resveratrol (Res) is known to inhibit adhesion of numerous malignancies though its effect on an adherence of ovarian cancer cells to peritoneal mesothelium remains undefined. Methods To address this issue, ovarian cancer cells (A2780, OVCAR-3, SKOV-3) were subjected to Res (10, 50, 100 μM), and then their adhesion to omentum-derived human peritoneal mesothelial cells (HPMCs) was assayed. Results The study showed that Res inhibits adhesion of all ovarian cancer cell lines investigated. More ...
Tópico(s): Cell Adhesion Molecules Research
2014 - Elsevier BV | Gynecologic Oncology
Joanna Jakubowska, Justyna Mikuła‐Pietrasik, Krzysztof Książek, H. Krawczyk,
We synthesised seven 2-aminestilbenes with methoxy substitents in reactions of dinitrostilbenes with sodium azide. In order to study the positioning of the nitro groups, the optimum structure of obtained stilbenes using the DFT B3LYP/6-311++G(2d,p) method was calculated. Very interesting aspect of this regioselectivity reaction is the fact that in all substrates and synthetized compounds the nitro groups in position 2 were not coplanar whereas the para -nitro groups were coplanar with respect to ...
Tópico(s): Click Chemistry and Applications
2014 - Hindawi Publishing Corporation | BioMed Research International
Patrycja Sosińska, Justyna Mikuła‐Pietrasik, Krzysztof Książek,
Normal human somatic cells have strictly limited proliferative capacity and reach a state of senescence when it becomes exhausted. It is believed that senescence is a response to extensive and irreparable DNA injury, localized in telomeric and/or non-telomeric regions of the genome. Main cause of this damage is oxidative stress, increasing due to deteriorated function of mitochondria. Senescent cells accumulate in tissues during aging, which is causatively linked with the development of various ...
Tópico(s): Genetics, Aging, and Longevity in Model Organisms
2016 - Index Copernicus International S.A. | Postępy Higieny i Medycyny Doświadczalnej
Curie-Skłodowskiej w Lublinie, długoletni kierownik Katedry Prawa Rzymskiego tego Uniwersytetu,
Tópico(s): Medieval European History and Architecture
2018 - Institute of History | Czasopismo Prawno-Historyczne