Book.
0000 - Gale Group | SAS Part 1
Chen‐Xu Liu, Zhong‐Jian Cai, Qiang Wang, Zhijie Wu, Qing Gu, Shu‐Li You,
... with LiOtBu (48.0 mg, 0.6 mmol), L7 (25.3 mg, 0.04 mmol), [Rh(C2H4) ... with 3,5-(MeO)2-C6H3-substituted ligand L7, the corresponding product 3aa was obtained in 88% ... Thus, 3,5-(MeO)2-C6H3-substituted ligand L7 was the optimal choice. With 2.0 equivalents ... as follows: [Rh(C2H4)2Cl]2 (5 mol%), L7 (20 mol%), and LiOtBu (3.0 equiv) in ... L5 THF – – 13 L6 THF 72 99 14 L7 THF 88 >99 15 L8 THF 68 99 ... L11 THF 55 −99 19 L12 THF – – 21b L7 THF 92 >99 22c L7 THF 97 (93)f >99 aReaction conditions: 1a ( ... 0 equiv), [Rh(C2H4)2Cl]2 (5 mol%), L7 (0.04 mmol), LiOtBu (0.6 mmol) in ...
Tópico(s): Metal-Organic Frameworks: Synthesis and Applications
2020 - Chinese Chemical Society | CCS Chemistry
J. SMITH., R. J., RADICAL., A METROPOLITAN., Q., A MEMBER OF THE ABOVE ROOM.,
... April 17., Court of Common Pleas, Westminster, April l7. Sparling V. Lynes., Rolls' Court, Tuesday, April 17. ...
1832 - Gale Group | TDA
Amal Senevirathne, Chamith Hewawaduge, John Hwa Lee,
Abstract The present study employs the Brucella abortus L7/L12 antigen in a Salmonella secretion platform and ... challenge in BALB/c mice. The highly conserved L7/L12 open reading frame was PCR amplified from ... beta-lactamase secretory signal. The plasmid constructs pJHL65::L7/L12 was then transformed into an attenuated Salmonella ... vector-only control, or the vaccine strain secreting L7/L12 antigen. Assessment of humoral and cell-mediated ... RT-PCR based cytokine profiling upon restimulation with L7/L12 purified antigen. Results indicate that immunization with ...
Tópico(s): Escherichia coli research studies
2020 - BioMed Central | Veterinary Research
Jonathan Northcroft, John Dugdale, Barbara Hall, Helena Frith Powell, Nigel Botherway, Guy Ritchie, Fred Redwood, S R, Jim Munro, Sally Brock, David Grayson, Helen Davies, Peter Whittle, Nicholas Rufford, John Peter, Professor Gideon Garter, Hamish Stevenson, Natalie Ayres, Jim Munro football editor, Andrew Longmore, Sue Harpley, John Waples Business Editor, S G, Nicolette Jones, Andrew Taylor, David Smith, David Cracknell Political Editor, Neil Wormald, Richard Green, E P, Clive Davis, Prunella Scales, K R, Dr Sam Banik, Richard Fletcher City Editor, Tony Allen-Mills, Simon Wilde, Martin James, Jane Thynne, Alyson Walsh, Robert Winnett, Dipesh Gadher Transport Correspondent, Irwin Stelzer, Adrian Furnham, Julian Rathbone, Robert Hewison, Peter Wilson, Rosie Millard, Tracey Emin, David Dougill, Chima Okezue, John Arlidge, Emma John, Christopher Hart, D D, Paul Spare, Cathy James, Tony Marcus, Stuart Barnes, Peter Jones, H C, Kamil Tchorek, Cally Law, Paul Forsyth, Hugh Mcllvanney, Michael Harold, Geraldine Hackett Education Correspondent, Hugh Canning, Andrew Stone, Jeremy Clarkson, Edward Porter, Peter Conradi, Victoria Segal, Nicola Smith, Nick Middleton, Stewart Lee, Fergus Garrett, Patricia Nicol, Sarah Dempster, Dave Pollard, Kevin Jackson, Richard Fletcher, Greg Struthers, Suzi Woolfson, Lydia Slater, Kate Hoey, Joe Klein, Jeff Potter, Andrew Porter Deputy Political Editor, Antony Jay, Paul Donovan, Ali Rifat, Frances Spalding, Paul Kimmage, Chris Woodhead, Jasper Gerard, Paul Driver, Garth Pearce, Chris West, John Follain, Professor David Coleman, Ian Hawkey, Stuart Andrews, Felipe Fernandez-Armesto, Alex Clark, David Leppard, Tim Rayment, Paul Durman, Shelley Von Strunckel, Mark Edwards, Heston Blumenthal, Susan Clark, Kira Cochrane, Richard Starks, Raymond Keene, C L, Gareth Huw Davies, Kate Saunders, Rod Liddle, David Cracknell, Robbie Hudson, Zoe Brennan, Stanley Stewart, Cosmo Landesman, Alice Douglas, Emma Smith, Stephen Jones, Dominic O'Connell, Peter Koenig, Louise Armitstead, Sarah-Kate Medical Correspondent, Uzi Mahnaimi, Nick Cain, Stephen Bleach, Adrienne Connors, Ines Sabalic, Natalie Graham, Catherine Wheatley, Lucy Atkins, Caroline Donald, S A, David Walsh, Miranda Seymour, David Emery, Jano Nottage, Kasha Harmer, Trevor Lewis, S P, Colin McDowell, Stephen Armstrong, John Elliott, M L, Victoria O'brien, Jonathan Leake Environment Editor, N R, Roland White, Richard Woods, John Prescott Mp, Sheryl Garratt, Shane Watson, Amrit Dhillon, Maurice Chittenden, Richard Brooks Arts Editor, Richard Rae, Jon Ungoed-Thomas, Michael Sheridan, Clare Francis, Michael Smith, Dileep Premachandran, Chris Haslam, Cynthia Lennon, Mark Kleinman, John Waples, Hunter Davies, David Parsons, Marek Kohn, Brian Glanville, Simon Jenkins, Dr Myles Bowen, Deirdre Fernand, Jonathan Carr-Brown, Roland Dobbs Professor, Jonathan Leake Science Editor, Jonathan Leake Science Correspondent, Jessica Bown, Monika Linton, Chrissy Iley, Justin Sparks, Danny McGuire, Sally Kinnes, Daniel Emery, Sophie Harrison, Stuart Allan, Lucretia Stewart, Ben Dowell, Minette Marrin, Dr Shaba Nabi, Gordon Airs, John Aizlewood, Charles Doidge, Mark Hodson, Andrew Frankel, Joe Lovejoy, Julia Baird, Ali Hussain, Jonathan Futrell, David Budworth, Hugh Pearman, Graham Norwood, Dan Cairns, P W, Christopher Bray, Waheed Saleem, Matt Roberts, David Smith Economics Editor, Tom Deveson, Janie Omorogbe, Sarah Baxter, Will Crove-White, Fleur Britten, Michael Carter, Matthew Goodman, Kate Spicer, Jason Dawe, Mark Barrett, Sian Griffiths, Karen Robinson, Helen Stewart,
... and fun to use Musical Fashionista Motorola SLVR L7—free with contract, or £200 at www. carphonewarehouse. ...
2005 - Gale Group | Sunday Times HA GDA
Haibo Liu, Xiaoxue Wu, Tan Jun, Xiao Li,
... mesiobuccal root of the second molar (L6 db –L7 mb ), and (iv) between the mesiodistal roots of the second molar (L7 mb –L7 db ). The buccal alveolar bone thickness, the narrowest ... the crest edge to the mandibular roots. The L7 mb –L7 db region had the thickest buccal alveolar bone ... buccal inter-radicular spaces were smallest in the L7 mb –L7 db region and greatest in the L6 db –L7 mb region. The distances from the implantation site ... 13 mm from the L6 region to the L7 region. Conclusions The L6 db –L7 mb region should be the first choice for ...
Tópico(s): Cleft Lip and Palate Research
2019 - Springer Nature | Progress in Orthodontics
Alfred Hawkins, R. F. A., Mr. W. H. Mallock, Leonard Rees, Lloyd's, L. Spence, Thos. Landers, Mr. Havelock Ellis, Mr. Daniel Frohman, Argus, Mrs. Mary Scharlieb, Bertram Williams L. D. S, Yarborough, Sir E. Ray Lankester, Mr. Welter Sichael, E. White, A. T. Spriggs, Alice Eeles, Mr. Sidney Low, S. W., Duchess, Lord Cromer, Sergius Cyon, Augusta A. Dawson-Dawson, H. L. K., Hun Hunter, Frank Rutter, F. G. Bettany, Edward Carson, A. Rubens, C. H. Bovill, H. R. S., E. L. J., R. L. Eagle, Col F. N. Maude C. B., Bishop welldon, Miss Cively Homilton, L. Van Vliet, George Bellamy, J. Stanley Holmes F. C. A, J. T. Grein, Subaltern, Paul A. Rubens, (Mrs) S. B. Stuart, Hymenopter, Gerald Biss, Frank Runohman, Dr. R. W. Scton Watson, H. R. M., Harold Cox,
... 4 Lost in 4 Days British Submarine Finishes L7 Only Seven Survivors Central News: More Violations Ships ...
1916 - Gale Group | Sunday Times HA GDA
Markus A. Carlson, Bassam G. Haddad, Amanda J. Weis, Colby S. Blackwood, Catherine Shelton, Michelle E. Wuerth, Justin D. Walter, Paul Spiegel,
Ribosomal protein L7/L12 is associated with translation initiation, elongation, and termination by the 70S ribosome. The guanosine 5′ triphosphate hydrolase ( ... factor G ( EF ‐G) requires the presence of L7/L12, which is critical for ribosomal translocation. Here, ... developed new methods for the complete depletion of L7/L12 from Escherichia coli 70S ribosomes to analyze the effect of L7/L12 on the activities of the GTP ase ... 3, IF 2, and LepA. Upon removal of L7/L12 from ribosomes, the GTP ase activities of ...
Tópico(s): RNA Research and Splicing
2017 - Wiley | FEBS Journal
Liz Dolan, Julia Llewellyn Smith, David Adams, Nick Nuttall Environment Correspondent, Alasdair Palmer, Ian Percival, Andrew Pierce, Anatol Lieven, Patricia Davies, Jane MacQuitty, Simon Barnes, Michael Scott Rohan, Jill Sherman Political Correspondent, Bill Frost, Neil Bennett, D. Major, Ronald Faux, Jeremy Laurence Health Services Correspondent, John Diamond, Giles Gordon, Philip Howard, Jonathan Mirsky and Dominic Kennedy, Hilary Finch, John Marriott, G. P., Ivan Lawrence, Steve Clarke, Clive Davis, Nicola Cole, Albert Dormer, Shirley Davenport, Nick Nuttall, Environment Correspondent, David Flusfeder, K. E., Kevin Eason, Colin Narbrough, Eve-Ann Prentice, Diplomatic Correspondent, Helen Simpson, Stuart Jones Tennis Correspondent, Gerald Davies, Geoff Brown, Joe Joseph, Robert Miller, Giles Coren, Michael Dynes Whitehall Correspondent, Melvyn Marckus, City Editor, Stewart Tendler Crime Correspondent, John Hopkins, Golf Correspondent, Peter Ball, Ruth Gledhill, S. L., Peter Rayner, W. B. Morgan, Raymond Keene Chess Correspondent, Tom Coughtrie, Peter Mandelson, Anne Caborn, Susan Grossman, John Wellman, Peter Bills, Peter Waymark, Robin Young, Rodney Milnes, Michael Clark, Richard Scott, Michael Hamlyn, Frances Bissell, Laurie Taylor, David Hands, Charles Bremner, John Warwick Montgomery, David Alexander, Jonathan Sacks, John Percival, Annie Rankin, Peter Barnard, John Reed, John Hennessy, Derwent May, J. M., Robert Béar, Sue Fox, Margaret Dibben, Richard Cork, George Rae, Stephen Anderton, John Woodcock, Tom Piper, Jimmy Smith, David Miller, Jill Insley, Barry Millington, P. J. Price, Sir Ranulph Fiennes, George Plumptre, David Toop, John Higgins, Kenneth MacKenzie, V. F., David Lyon and Michael Hartnall, Raymond Keene, David Robinson, John Russell Taylor, Emma Wilkins, Andrew Lowe-Watson, Rhodes Boyson, Stephanie Lewis, Michael Dynes, Whitehall Correspondent, Phil McLennan, Richard Evans, Nigel Hawkes, Oliver Holt, David Powell, Athletics Correspondent, Jonathan Shepherd, Nigel Hawkes, Science Editor, Helen Nowicka, Nigel Barraclough, Philip Webster, Political Editor, Mark Lucas, Kate Alderson, Eric Saywell, Wilfrid M. Moss, Julian Muscat, Nicholas Wood, Chief Political Correspondent, Richard Duce, Martin Waller, Deputy City Editor, John Goodbody, M. A. Symonds, Terence Lee, Jane Bidder, Ross Tieman Industrial Correspondent, Sara McConnell, Rob Hughes, Football Correspondent, Nicholas Watt Ireland Correspondent, Brian MacArthur, Craig Lord, Mel Webb, Gerald Larner, Matthew Parris, Mike Rosewell, Richard Hemmings Director, Frank Jeffery, Christopher Walker, Middle East Correspondent, John Shaw, Keith Kemp, Neil Murray, Peter Riddell, Ben MacIntyre, Barry Ward, Ian Brodie, Russell Twisk, Tony Dawe, Alan Lee Cricket Correspondent, David Sinclair, Simon Jenkins, Stephen Pettitt, Kate Bassett, Robert Kee, Christopher Thomas and Jonathan Mirsky, Stuart Hills, Benedict Nightingale, Tom Rhodes, Katie Sotheran, Jill Sherman, Political Correspondent, Edward Gorman and Tim Judah, Richard Morrison, Patricia Tehan Banking Correspondent, Lindsay Cook Deputy Business Editor, Martin Waller Deputy City Editor, Andrew Finkel, John Phillips, Mike Golding, Matthew Bond, Matthew Beard, Hilary Kingsley, Christopher Irvine,
... the Portuguese Grand Prix The Times Lease Plan L7 Company Car Driver 1994 Wave goodbye to little ...
1994 - Gale Group | TDA
Tatsuya Yamada, Yoshihiro Adachi, Masahiro Fukaya, Miho Iijima, Hiromi Sesaki,
... which is expressed from the Purkinje cell–specific L7 promoter.13Kageyama Y. Zhang Z. Roda R. Fukaya ... 1464-1469Crossref PubMed Scopus (416) Google Scholar The L7-Cre mice were obtained from the Jackson Laboratory ( ... PubMed Scopus (136) Google Scholar By breeding Rip3+/−::L7-Cre+/−::Drp1flox/flox mice and Rip3+/−::Drp1flox/flox ... control (Drp1flox/flox), Rip3-KO (Rip3−/−::Drp1flox/flox), L7-Drp1-KO (L7-Cre+/−::Drp1flox/flox), and double-KO (Rip3−/−::L7-Cre+/−::Drp1flox/flox) mice. The Drp1flox/flox mice ... aperture) objective. We generated control, Rip3-KO (Rip3−/−), L7-Drp1-KO (L7-Drp1flox/flox), and double-KO ( ...
Tópico(s): Cellular transport and secretion
2016 - Elsevier BV | American Journal Of Pathology
Francisco A. Velloso, José D. Simoes, Abel Francisco, João D'olivelra Amaral, Joaqaim R. Nogueira, Antonio C. D'almeida,
... Wages from May Next for Carpenterers Shall Be L7 10s, "Post" for Printing, Keating's Powder, Multiple ...
1912 - Gale Group | NCCO EuropeAfrica
Munusamy Madhaiyan, Cao Hu, Joseph Jegan Roy, S.-J. Kim, Hang-Yeon Weon, Soon-Wo Kwon, Lianghui Ji,
Four orange-pigmented isolates, L7-456, L7-484(T), L9-479 and L9-753(T), originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using ... to the genus Aureimonas. In these analyses, strain L7-484(T) appeared to be most closely related ... identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753(T) were ... A. ureilytica 5715S-12(T) (97.5 %). Both L7-484(T) and L9-753(T) contained Q- ... also present. The DNA G+C contents of L7-484(T) and L9-753(T) were 66. ...
Tópico(s): Plant Disease Resistance and Genetics
2012 - Microbiology Society | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Sharon Anavi‐Goffer, Daniel Fleischer, Dow P. Hurst, Diane L. Lynch, Judy Barnett‐Norris, Shanping Shi, Deborah L. Lewis, Somnath Mukhopadhyay, Allyn C. Howlett, Patricia H. Reggio, Mary E. Abood,
... transduction capabilities of CB1 with those of an L7.60F mutation and an L7.60I mutation that mimics the CB2 sequence. The ... 5′-3-O-(thio)triphosphate binding assay. The L7.60F receptor exhibited attenuated stimulation by agonists WIN- ... 55,940 but not HU-210, whereas the L7.60I receptor exhibited impaired stimulation by all agonists ... coupling to Gαi3 but not Gα0A in the L7.60I mutant, whereas the reduction in the time course for the L7.60F mutant was governed by Gαi3. Furthermore, Gαi3 ... transduction capabilities of CB1 with those of an L7.60F mutation and an L7.60I mutation that ...
Tópico(s): Pancreatic function and diabetes
2007 - Elsevier BV | Journal of Biological Chemistry
Roxana Nechifor, Marat B. Murataliev, Kevin S. Wilson,
Protein L7/L12 of the bacterial ribosome plays an important role in activating the GTP hydrolytic activity of elongation ... translocation during protein synthesis. Previously, we cross-linked L7/L12 from two residues (209 and 231) flanking ... and smaller defects in ribosomal translocation. Removal of L7/L12 from the ribosome strongly reduced the activities ... interactions between helix AG′ of EF-G and L7/L12 of the ribosome. Protein L7/L12 of the bacterial ribosome plays an important ... translocation during protein synthesis. Previously, we cross-linked L7/L12 from two residues (209 and 231) flanking ...
Tópico(s): Bacterial Genetics and Biotechnology
2007 - Elsevier BV | Journal of Biological Chemistry
Miwako Iwai, Takayuki Michikawa, Ivan Bosanac, Mitsuhiko Ikura, Katsuhiko Mikoshiba,
... and tenth loop regions (L1, L3, L4, L5, L7, L8, and L10, respectively, supplemental Fig. S2) (7Bosanac ... 3-specific loop regions, L1, L3, L4, L5, L7, L8, and L10, were substituted with residues appearing ... 5A). These results showed that both L5 and L7 are required for the suppression of IP3 binding ... specific amino acid residues within both L5 and L7 in T604m3 were simultaneously changed to the residues ... IP3R1 (supplemental Fig. S2). This mutant protein, (L5,L7)m1/T604m3, exhibited an apparent dissociation constant (80. ...
Tópico(s): Receptor Mechanisms and Signaling
2007 - Elsevier BV | Journal of Biological Chemistry
Deyan Luo, Peng Li, Li Xing, Guangyu Zhao, Wei Shi, Zhang Songle, Xiliang Wang,
... 2 A DNA vaccine comprising the Brucella ribosomal L7/L12 gene has been demonstrated to induce significant ... study, we constructed the plasmid containing two genes, L7/L12 and P39, to evaluate its protective capacity ... ad libitum. Plasmids and protein Plasmids pcDNA3.1(+)-L7/L12,5 pcDNA3.1(+)-P39 and fusion protein L7/L12-P39 (rL7/L12-P39), crude Brucella abortus ... kept in our laboratory. Construction and preparation of L7/L12-P39 DNA vaccine PCR was performed using ... from gene bank. The primer sequences of the L7/L12 were 5′-TAGGTACCATGGCTGATCTCGC-3′ (primer 1) and ...
Tópico(s): Escherichia coli research studies
2006 - Lippincott Williams & Wilkins | Chinese Medical Journal
Akiko Hagiya, Takao Naganuma, Yasushi Maki, Jun Ohta, Yukiko Tohkairin, Tomomi Shimizu, Takaomi Nomura, Akira Hachimori, Toshio Uchiumi,
... coli core 50 S subunits deficient in L10·L7/L12 complex and L11. Analysis of incorporation of ... coli core 50 S subunits deficient in L10·L7/L12 complex and L11. Analysis of incorporation of ... prokaryotic ribosomes constitute a characteristic pentameric complex, L10(L7/L12)2(L7/L12)2 (4Pettersson I. Hardy S.J. Liljas ... PubMed Scopus (41) Google Scholar), in which two L7/L12 homodimers bind to the C-terminal regions ... P proteins) are counterparts of prokaryotic L10 and L7/L12, respectively, although P1 and P2 are related ...
Tópico(s): Viral Infections and Immunology Research
2005 - Elsevier BV | Journal of Biological Chemistry
Eduard V. Bocharov, Alexander G. Sobol, Konstantin V. Pavlov, Dmitry M. Korzhnev, Victor Jaravine, A.T. Gudkov, Alexander S. Arseniev,
... structure and internal dynamic properties of ribosomal protein L7 from Escherichia coli were derived. The structure of L7 dimer in solution can be described as a ... region. Combining structural data for the ribosomal protein L7/L12 from NMR spectroscopy and x-ray crystallography, ... molecular switch, initiating "ratchet-like" motions of the L7/L12 stalk with respect to the ribosomal surface ... provides useful insights into the role of protein L7/L12 in the functioning of the ribosome. Based ... structure and internal dynamic properties of ribosomal protein L7 from Escherichia coli were derived. The structure of ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2004 - Elsevier BV | Journal of Biological Chemistry
Takaomi Nomura, Ruriko Mochizuki, Eric R. Dabbs, Yoshihiro Shimizu, Takuya Ueda, Akira Hachimori, Toshio Uchiumi,
... a mutation (Ser15 to Phe) in ribosomal protein L7/L12 was isolated among revertants of a streptomycin-dependent strain. In the crystal structure of the L7/L12 dimer, residue 15 within the N-terminal ... approaches. Gel electrophoretic analysis showed that the Phe15-L7/L12 variant had reduced ability in binding to ... 0.05% of nonionic detergent. Mobility of Phe15-L7/L12 on gel containing the detergent was very ... of an extended structural state of the mutant L7/L12. Ribosomes isolated from LL103 cells contained a ...
Tópico(s): Bacterial Genetics and Biotechnology
2003 - American Chemical Society | Biochemistry
... the truncated PrP under the control of the L7 promoter We expressed PrP devoid of residues 32– ... under the control of the Purkinje cell-specific L7 promoter (Oberdick et al., 1990) in Zürich I ... under the control of the Purkinje cell-specific L7 promoter. Hemizygous mice (L7-ΔPrP/−) of the lines TgF13 (n = 10), TgF32 ( ... under the control of the Purkinje cell-specific L7 promoter. Immunostaining for calbindin (top row), PrP (middle ... GFAP (lower row) of sections through cerebella of L7-ΔPrP mice (TgF13) are shown. Sections were immunostained ...
Tópico(s): CRISPR and Genetic Engineering
2003 - Springer Nature | The EMBO Journal
Produção Nacional
Revisado por pares
Luciana Ribeiro, Vasco Azevedo, Yves Le Loir, Sérgio C. Oliveira, Yakhya Dièye, Jean-Christophe Piard, Alexandra Gruss, Philippe Langella,
... in humans. The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the ... lactic acid bacteria (LAB) as a carrier. The L7/L12 gene was expressed in Lactococcus lactis , the ... under the nisin-inducible promoter. Using different signals, L7/L12 was produced in cytoplasmic, cell-wall-anchored, and secreted forms. Cytoplasmic production of L7/L12 gave a low yield, estimated at 0. ... a signal peptide resulted in increased yield of L7/L12 to 3 mg/liter; secretion efficiency (SE) ... mature moiety of the staphylococcal nuclease (Nuc) and L7/L12 further increased yield to 8 mg/liter. ...
Tópico(s): Bacteriophages and microbial interactions
2002 - American Society for Microbiology | Applied and Environmental Microbiology
Thomas Ilg, Monika Demar, Dorothee Harbecke,
... LT6 (directed against [-6Galβ1–4Manα1PO4-]x,x = unknown), L7.25 and AP3 (directed against [Manα1–2]0– ... electrophoresis (SDS-PAGE); immunoblotting using the mAbs LT6, L7.25, LT17, LT8.2 (23Ilg T. Harbecke D. ... Fig. 5 B). The anti-mannooligosaccharide cap antibody L7.25 (Fig. 1 A) recognizes LPG and a ... lanes 2). In the PG repeat-negative mutant, L7.25 recognizes the full set of PPGs, whereas ... front of the gel, a broad band of L7.25-reactive material is visible that is absent ... PubMed Google Scholar), which is also reactive with L7.25. 2T. Ilg, unpublished results. FACS analysis (Fig. ...
Tópico(s): Eosinophilic Disorders and Syndromes
2001 - Elsevier BV | Journal of Biological Chemistry
Dagmar Mohr, Wolfgang Wintermeyer, Marina V. Rodnina,
... major stimulating interaction is that with ribosomal protein L7/12, which in experiments with isolated protein stimulates ... al., 2000). On the ribosome, the effect of L7/12 may be augmented by other interactions (see Discussion). The single, highly conserved arginine of L7/12 is not essential for the stimulation, indicating that the function of L7/12 may be to induce or stabilize the ... 1998; Stark et al., 2000), which comprises protein L7/12, based on immunoelectron microscopic and biochemical data ( ... al., 1999). In contrast, the strong stimulation by L7/12 of the GTPase activity of EF-G ...
Tópico(s): Bacteriophages and microbial interactions
2000 - Springer Nature | The EMBO Journal
O I Griaznova, Robert R. Traut,
Escherichia coli ribosomal protein L10 binds the two L7/L12 dimers and thereby anchors them to the ... to define the binding sites for the two L7/L12 dimers and then to make and test ... the proteins to bind both dimers of protein L7/L12. The L10 variant with deletion of 10 amino acids bound one L7/L12 dimer in solution and when reconstituted into ribosomes promoted the binding of only one L7/L12 dimer to the ribosome. The ribosomes that contained a single L7/L12 dimer were homogeneous by gel electrophoresis where ...
Tópico(s): Bacterial Genetics and Biotechnology
2000 - American Chemical Society | Biochemistry
Hideki Kitaura, M Kinomoto, Takeshi Yamada,
... resistance. Two such proteins corresponded to ribosomal proteins L7 and L12. The mixture of these proteins L7/L2 induced a strong delayed-type hypersensitivity reaction. ... other unidentified proteins. It is well known that L7 and L12 are encoded by the same gene ... the N-terminus of L12 converting it to L7 in Escherichia coli. L12, but not L7, was found in two-dimensional electrophoresis of BCG ribosomes, although we found two proteins corresponding to L7 and L12 in PPDs and a native culture ... compared the delayed-type hypersensitivity reaction elicited by L7/L12 derived from a culture filtrate of BCG ...
Tópico(s): Pneumonia and Respiratory Infections
1999 - Wiley | Scandinavian Journal of Immunology
Michael Justice, Theresa Ku, Ming‐Jo Hsu, Karen Carniol, Dennis M. Schmatz, Jennifer Nielsen,
... structure that is similar to the L10 and L7/L12 complex inEscherichia coli ribosomes. The conservation of ... PubMed Scopus (77) Google Scholar) ribosomes. The prokaryotic L7/L12 ribosomal proteins have been studied extensively by ... the stalk proteins and elongation factors. Mutations in L7/L12 perturb both EF-Tu and EF-G ... between EF-Tu and EF-G and the L7/L12 complex (reviewed in Ref. 16Czworkowski J. Moore ... links are observed between the EF-G and L7/L12 proteins in the presence of the nonhydrolyzable ... 71: 1277-1280Crossref PubMed Scopus (60) Google Scholar). L7/L12 proteins in the EF-G-fusidic acid- ...
Tópico(s): Mycotoxins in Agriculture and Food
1999 - Elsevier BV | Journal of Biological Chemistry
Uma Chandran, Barbour S. Warren, Christopher T. Baumann, Gordon L. Hager, Donald Defranco,
... Cruz Biologicals (Santa Cruz, CA). The sequences of L7, L73M, and L7M oligonucleotides are shown in Fig. ... Scholar). 0.05 μm32P-labeled double-stranded oligonucleotide (L7 or L7M) was incubated with 1× gel shift ... above. 0.05 μm32P-labeled double-stranded oligonucleotide (L7 or L7M) was incubated with 1× Oct-1 ... 1 also binds the distal GnRH nGRE oligonucleotide L7, although with lower affinity than to the nGRE ... formed on this nGRE (i.e. complex C1-L7) contains Oct-1 and GR (25Chandran U.R. ... compared with the Oct-1-containing complex, C1-L7, formed on the distal nGRE (Fig. 3 A, ...
Tópico(s): Hypothalamic control of reproductive hormones
1999 - Elsevier BV | Journal of Biological Chemistry
Yvonne Berghöfer-Hochheimer, Christian Zurek, Stefan Wölfl, Peter Hemmerich, Thomas Münder,
... hybrid system, we identified the previously described protein L7, that specifically interacted with the VDR in the ... Deletion analysis indicated, that the N-terminus of L7, which harbours a basic region leucine zipper like ... with the VDR. Binding assays with purified GST-L7 demonstrated, that L7 specifically pulled down the VDR, that was either ... endogenously contained in the cell line U937. Interestingly, L7 inhibited ligand-dependent VDR-RXR heterodimerization, when constitutively expressed in yeast. We also demonstrate that L7 repressed binding of VDR-RXR heterodimers to a ...
Tópico(s): Vitamin D Research Studies
1998 - Wiley | Journal of Cellular Biochemistry
Meirav Bar Shimon, Rivka Goldshleger, Steven J.D. Karlish,
... between trans-membrane segments M7 and M8 and L7/8. Minor cleavages are also detected in loops ... and spatial organization. The two cleavage sites in L7/8 of the α subunit and two cleavage ... β subunit identify interacting segments of the subunits. L7/8 is also close to L9/10 and ... between trans-membrane segments M7 and M8 and L7/8. Minor cleavages are also detected in loops ... and spatial organization. The two cleavage sites in L7/8 of the α subunit and two cleavage ... β subunit identify interacting segments of the subunits. L7/8 is also close to L9/10 and ...
Tópico(s): Electrochemical Analysis and Applications
1998 - Elsevier BV | Journal of Biological Chemistry