Barbara Ruaro, Alberto Sulli, Elisa Alessandri, Carmen Pizzorni, Giorgia Ferrari, Maurizio Cutolo,
... a new technique, the laser speckle contrast analysis (LASCA), in systemic sclerosis (SSc) patients showing different patterns of nailfold microangiopathy. Correlations between LASCA and single laser Doppler flowmetry (LDF) analysis were ... healthy subjects were enrolled. PBP was evaluated using LASCA and LDF. Scleroderma patterns and microangiopathy evolution score ( ... assessed by nailfold videocapillaroscopy (NVC).As detected by LASCA and LDF, PBP was lower in SSc patients ... p=0.006 and p=0.002 for LASCA and LDF, respectively). A positive correlation was detected ...
Tópico(s): Diagnosis and Treatment of Venous Diseases
2013 - BMJ | Annals of the Rheumatic Diseases
Peng Miao, Nan Li, Nitish V. Thakor, Shanbao Tong,
... speckle contrast analysis (i.e.spatial or temporal LASCA) can be derived as the spatial or temporal estimators respectively.Both spatial LASCA and temporal LASCA suffer from noise due to insufficient statistics and ... in either spatial or temporal domain.Furthermore, either LASCA results in a reduction of spatial or temporal ... vitro study, random process estimator outperforms either spatial LASCA or temporal LASCA by providing much higher SNR (random process estimator vs. spatial LASCA vs. temporal LASCA: 33.64±6.87( . . .mean ...
Tópico(s): Climate Change and Health Impacts
2009 - Optica Publishing Group | Optics Express
David Allan, Nachiappan Chockalingam, Roozbeh Naemi,
... lower limb blood flow. Laser Speckle Contrast Analysis (LASCA) has been identified as the current gold standard ... obtained from this technique compare against that of LASCA at plantar skin. Therefore, the aim of this ... plantar surface of the foot using iPPG and LASCA. Perfusion at six plantar locations (Hallux, 1st 3rd ... metatarsal heads, midfoot, heel) was simultaneously measured using LASCA and iPPG in 20 healthy participants. Skin thickness ... medium strength between the perfusion values measured with LASCA and iPPG for most tested sites. No improvement ...
Tópico(s): Infrared Thermography in Medicine
2021 - Taylor & Francis | Journal of Medical Engineering & Technology
Maurizio Cutolo, Amber Vanhaecke, Barbara Ruaro, Ellen Deschepper, Claudia Ickinger, Karin Melsens, Yves Piette, Amelia Chiara Trombetta, Filip De Keyser, Vanessa Smith,
... on the reliability of laser speckle contrast analysis (LASCA) to measure the peripheral blood perfusion (PBP) in ... investigating the intra- and inter-rater reliability of LASCA. A systematic search was performed in 3 electronic ... SSc patients and 30 healthy subjects (HS) underwent LASCA assessment. Intra-rater reliability was assessed by having ... 87. The identified literature regarding the reliability of LASCA measurements reports good to excellent inter-rater agreement. ... very pilot study could confirm the reliability of LASCA measurements with good to excellent inter-rater agreement ...
Tópico(s): Dermatologic Treatments and Research
2018 - Elsevier BV | Autoimmunity Reviews
Dainis Jakovels, Uldis Rubīns, Jānis Spīgulis,
Laser speckle contrast analysis (LASCA) offers a non-contact, full-field, and real-time mapping of capillary blood flow and can be considered as an ... on development of non-contact PPG imaging (PPGI). LASCA and PPGI techniques are simpler and cheaper compared with LDPI. LASCA technique has been implemented in several commercial instruments. ... In this work we demonstrate simple implementation of LASCA and PPG imaging technique for primary low-cost ... 650 nm laser diode module is used for LASCA illumination, and white LEDs are illuminators for PPG ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2013 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
... we have developed the laser speckle contrast analysis (LASCA) technique. The LASCA method is a spatial domain method, based on ... and velocity. First, we present an improved naive LASCA algorithm with the running time O(k 2 ... the subimage considered. Then, we describe a fast LASCA algorithm, which takes time O(kn) to calculate ... the fast sequential algorithm to design the first LASCA parallel algorithm to run on the CREW PRAM ... takes only about one second using the fast LASCA algorithm. Furthermore, our parallel algorithm is easily implemented ...
Tópico(s): Textile materials and evaluations
1998 - SPIE | Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Barbara Ruaro, Cosimo Bruni, Barbara Wade, Elisa Baratella, Paola Confalonieri, Caterina Antonaglia, Pietro Geri, Marco Biolo, Marco Confalonieri, Francesco Salton,
Laser speckle contrast analysis (LASCA) is a non-contact technique able to quantify peripheral blood perfusion (PBP) over large skin areas. LASCA has been used to study hand PBP in ... sclerosis (SSc) and systemic lupus erythematosus (SLE) and LASCA showed that PBP was significantly lower in these ... subjects (HS). Moreover, it has been demonstrated that LASCA is a safe technique also able to monitor ... during systemic and local treatment. The use of LASCA, coupled with reactivity tests is commonplace in the ... as specific tests of endothelium function. In conclusion, LASCA is a safe, non-contact reliable instrument for ...
Tópico(s): Infrared Thermography in Medicine
2021 - Frontiers Media | Frontiers in Physiology
Eric Pion, Claudia Asam, Anna-Lena Feder, Oliver Felthaus, Paul I. Heidekrueger, Lukas Prantl, Silke Haerteis, Thiha Aung,
... blood perfusion, based on laser speckle contrast analysis (LASCA). Aim of the present study was to utilize ... allowing real-time observation of the microcirculation via LASCA. Fertilized chicken eggs were grafted with embryonal/alveolar ... was measured before and after tumor growth using LASCA. The procedure is accelerated and simplified through the ... tumors exhibited satisfactory growth processes on the CAM. LASCA visualized microcirculation accurately and enabled an extensive investigation ... the CAM than the controls. The utilization of LASCA for the investigation of tumor angiogenesis within the ...
Tópico(s): Photoacoustic and Ultrasonic Imaging
2020 - Elsevier BV | Microvascular Research
S. Ragol, Itay Remer, Yaron Shoham, Sivan Hazan, Udi Willenz, Igor Sinelnikov, V. V. Dronov, Lior Rosenberg, A. Bilenca,
Laser speckle contrast analysis (LASCA) is an established optical technique for accurate widefield visualization of relative blood perfusion when no or minimal scattering from static ... elements is present, as demonstrated, for example, in LASCA imaging of the exposed cortex. However, when LASCA is applied to diagnosis of burn wounds, light ... flow for burns of different severity. Unlike conventional LASCA systems that employ scientific or industrial-grade cameras, our LASCA system is realized here using a camera phone, ...
Tópico(s): Bee Products Chemical Analysis
2015 - SPIE | Journal of Biomedical Optics
Peng Miao, Minheng Li, Hugues Fontenelle, Anastasios Bezerianos, Yihong Qiu, Shanbao Tong,
Laser speckle contrast analysis (LASCA) has been demonstrated as a full-field method for imaging the cerebral blood flow (CBF). However, conventional LASCA is limited to extremely low dynamic range because ... CBF. In this study, we proposed an enhanced LASCA (eLASCA) method to improve the dynamic range of LASCA based on monotonic point transformation. In investigating the ... much more significant decrease of relative CBF than LASCA (hypothermia: 189% versus 137%, postrewarming: 151% versus 119%). ... 009) of CBF change after the rewarming than LASCA (p=0.013). In addition, eLASCA greatly improves ...
Tópico(s): Infrared Thermography in Medicine
2008 - Institute of Electrical and Electronics Engineers | IEEE Transactions on Biomedical Engineering
Ingemar Fredriksson, Marcus Larsson,
... Doppler flowmetry (LDF) and laser speckle contrast analysis (LASCA) both utilize the spatiotemporal properties of laser speckle ... describing how the LDF power spectrum and the LASCA contrast can be calculated from each other, and ... to show how perfusion estimates for LDF and LASCA are affected by changes in blood concentration and ... that perfusion estimates from conventional single exposure time LASCA are in general more sensitive to changes in ... one can conclude that those drawbacks with the LASCA technique can be overcome using a multiple exposure ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2016 - SPIE | Journal of Biomedical Optics
Jonathan I. Brauer, Iwona B. Beech, Jan Sunner,
... as laser ablation and solvent capture by aspiration (LASCA) is presented and its performance demonstrated using selected, unaltered biological materials. LASCA employs a pulsed 2.94 μm laser beam ... high mass resolution Q-ToF mass spectrometer. The LASCA system separates the sampling and ionization steps in ... from a single ablation spot (pixel). Using the LASCA platform, rapid (6 s per pixel), high sensitivity, ...
Tópico(s): Ion-surface interactions and analysis
2015 - American Chemical Society | Journal of the American Society for Mass Spectrometry
Ferenc Domoki, Dániel Zölei, Orsolya Oláh, Valéria Tóth‐Szűki, B. Hopp, Ferenc Bari, Tomi Smausz,
A new laser speckle-contrast analysis (LASCA) technique based on multi-exposure imaging was employed to simultaneously study pial arteriolar responses with cerebrocortical perfusion changes to various vasodilator (5– ... proportional to blood flow velocity by two different LASCA techniques: our novel multi-exposure or a single ... ms) approach. 1/τ values yielded by different LASCA techniques were not significantly different at most points. LASCA easily detected both increases and decreases in cortical ... velocity and cross sectional area changes. In summary, LASCA emerges as an appealing method to simultaneously study ...
Tópico(s): Thermal Regulation in Medicine
2012 - Elsevier BV | Microvascular Research
Nils Hecht, Johannes Woitzik, Jens P. Dreier, Peter Vajkoczy,
... first clinical experience with laser speckle contrast analysis (LASCA) as a complementary imaging tool for the noninvasive ... study was to address the general feasibility of LASCA in terms of handling and image quality and ... of hemodynamic compromise and complex aneurysms. The portable LASCA device was centered over the surgical field, and ... graft and subsequent flow initiation through the bypass, LASCA allowed immediate visualization and measurement of relative CBF in excellent spatiotemporal resolution. Conclusions In this study LASCA offered noninvasive and rapid intraoperative assessment of relative ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2009 - American Association of Neurological Surgeons | Neurosurgical FOCUS
Georg Royl, Christoph Leithner, Heike Sellien, Jan Philipp Müller, Dirk Megow, Nikolas Offenhauser, Jens Steinbrink, Matthias Kohl‐Bareis, Ulrich Dirnagl, Ute Lindauer,
Laser Speckle Contrast Analysis (LASCA), a novel, high-resolution blood flow imaging method, was performed on rat somatosensory cortex during functional activation. In the same ... results show that: 1. CBF changes measured by LASCA or LDF are nonlinearly dependent on the magnitude ... The magnitude of relative CBF changes measured by LASCA and LDF shows a strong correlation. 3. LASCA imaging localizes the highest relative changes of CBF ... contribution by larger vessels. This study demonstrates that LASCA is a reliable method that provides 2D-imaging ...
Tópico(s): Optical Imaging and Spectroscopy Techniques
2006 - Elsevier BV | Brain Research
Carlo Alberto Cutolo, A. Cere, P. I. kander Toma, Tommaso Cannavacciuolo, Chiara Toma, S. Balito, Veronica Gerli, Vanessa Smith, Alberto Sulli, Sabrina Paolino, Emanuele Gotelli, Carlo Enrico Traverso, Massimo Nicolò, Maurizio Cutolo, Elvis Hysa,
... by nailfold videocapillaroscopy (NVC), laser speckle contrast analysis (LASCA), and optical coherence tomography angiography (OCTA). The enrollment ... rheumatological and ophthalmological examinations, as well as NVC, LASCA, and OCTA analysis on the same day at ... NVC) and the mean perfusion of fingers (at LASCA) with the retinal and choroidal perfusion (at OCTA) ( ... 67, p = 0.001). Finally, the combination of LASCA and OCTA parameters showed a significant discrimination capacity ...
Tópico(s): Systemic Lupus Erythematosus Research
2023 - Springer Science+Business Media | Rheumatology International
Zean Tian, Zhongyang Zhang, Jiang Xiao, Feng Wei, Ping Shen, Fan Wu,
... tool based on the largest standard cluster analysis (LaSCA), which satisfies the three essential requirements for general ... identification, and injective representation. The specific functionalities of LaSCA include the directed graph model of complex systems, ... treat atoms as spheres, icosahedrons, tetrahedrons, or points. LaSCA can process more than 1.2 million atoms ...
Tópico(s): Machine Learning in Materials Science
2023 - Multidisciplinary Digital Publishing Institute | Metals
Onega V. Ulianova, Sergey S. Ulyanov, Sergey Zaytsev, Yury V. Saltykov, Alexander S. Ulyanov, Valentina Feodorova,
... Typing (MLST). This method is based on s-LASCA imaging ( s patial La ser S peckle C ... coordinates for GB-speckles , processed by the s-LASCA method. A very high sensitivity and accuracy of ... model. The high efficiency of usage of s- LASCA -imaging of GB-speckles has been shown. The ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2021 - Public Library of Science | PLoS ONE
Marco Di Battista, Riccardo Morganti, Eva Tani, Mattia Da Rio, Alessandra Della Rossa, Marta Mosca,
... healthy controls (HC) using laser speckle contrast analysis (LASCA). Adult consecutive SSc patients and HC were enrolled. ... blood perfusion of the hands was evaluated by LASCA, subsequently obtaining 3 different regions of interest: from ... in SSc with great sensibility and specificity using LASCA, the best non-invasive imaging technique for the dynamical evaluation of peripheral perfusion. LASCA-PDG appears also as a tool able to ...
Tópico(s): Diagnosis and Treatment of Venous Diseases
2021 - Elsevier BV | Microvascular Research
Albin Stjernbrandt, Bodil Björ, Hans Pettersson, Ronnie Lundström, Ingrid Liljelind, Tohr Nilsson, Jens Wahlström,
... sensory testing (QST), and laser speckle contrast analysis (LASCA). Methods: Eight women and four men, ages 22– ... the dorsum of the hand was measured using LASCA, at baseline, after two-minute 12°C water ... One subject (8%) had impaired thermal perception thresholds. LASCA at baseline showed absent proximal-distal perfusion gradients ... cold sensitivity, which challenges the neurosensory pathophysiological hypothesis. LASCA indicated disturbances in microvascular regulation and could prove ...
Tópico(s): Biomedical and Chemical Research
2020 - Taylor & Francis | International Journal of Circumpolar Health
Haiying Cheng, Qingming Luo, Shaoqun Zeng, Xing‐Qiu Chen, Jian Cen, Hui Gong,
... and compared with current laser speckle contrast analysis (LASCA); the comparison indicates that the spatial resolution of ... is five times higher than that of current LASCA. Cerebral blood flow under different temperatures was investigated ... modified LSI. Compared with the results obtained by LASCA, the blood flow map obtained by the modified ... much higher spatial resolution than that of current LASCA and other laser Doppler perfusion imaging methods.
Tópico(s): Climate Change and Health Impacts
2003 - SPIE | Journal of Biomedical Optics
... healthy controls (HC) using laser speckle contrast analysis (LASCA).Adult consecutive SSc patients and HC were enrolled. ... blood perfusion of the hands was evaluated by LASCA, subsequently obtaining 3 different regions of interest: from ... in SSc with great sensibility and specificity using LASCA, the best non-invasive imaging technique for the dynamical evaluation of peripheral perfusion. LASCA-PDG appears also as a tool able to ...
Tópico(s): Infrared Thermography in Medicine
1974 - Elsevier BV | Microvascular Research
Onega V. Ulianova, Alexander S. Ulyanov, Sergey Zaytsev, Yury V. Saltykov, Sergey S. Ulyanov, Valentina Feodorova,
... GB-speckles have been processed using the s-LASCA (laser speckle contrast analysis) technique. Statistical properties of s-LASCA images of GB-speckles have been analyzed. It is shown that application of the s-LASCA technique allows us to essentially increase the the ...
Tópico(s): Thermoregulation and physiological responses
2020 - IOP Publishing | Laser Physics Letters
Dániel Zölei-Szénási, S. Czimmer, T. Smausz, Ferenc Domoki, B. Hopp, Lajos Kemény, Ferenc Bari, I. Iványi,
Laser Speckle Contrast Analysis (LASCA) has been proven to be a highly useful tool for the full-field determination of the blood perfusion of a ... Here we present two relatively simple extensions of LASCA for these problems. The application of multiple autocorrelation ...
Tópico(s): Infrared Thermography in Medicine
2015 - Springer Nature | Journal of the European Optical Society Rapid Publications
Marcin Gierek, Beata Bergler‐Czop, Anna Słaboń, Wojciech Łabuś, Gabriela Ochała-Gierek,
Laser speckle contrast analysis (LASCA) is a quite new and unique device which can find applications in multiple diagnostic approaches in dermatology and dermatosurgery. LASCA can be used in multiple approaches. We would ... to make the world's first presentation of LASCA usefulness in hidradenitis suppurativa (HS) surgery based on a case series.To evaluate the usefulness of LASCA in the surgical treatment of HS.In HS ... for Burns Treatment in Siemianowice Śląskie with specific LASCA findings.As a result of the LASCA examination, ...
Tópico(s): Chemotherapy-related skin toxicity
2023 - Termedia Publishing House | Advances in Dermatology and Allergology
Han Si-Yi, Ling Wang, Haibo Yu, Yanhua Gou, Weizheng Zhong, Xingxian Huang, Zhang Shao-Yun, Yongfeng Liu, Yirong Chen,
... In the trial, the laser speckle contrast analysis (LASCA) technology will be applied to monitor the microcirculation ... will also explore the objectivity and effectiveness of LASCA in the evaluation of facial paralysis (FP), and ... provide evidence for the feasibility of using the LASCA technique as a typing diagnosis of FP in ...
Tópico(s): Vestibular and auditory disorders
2021 - Wolters Kluwer | Medicine
... small fiber functions using laser speckle contrast analysis (LASCA). Methods Axon flare responses, which reflect small fiber ... flare responses (temporal/spatial characteristics) were measured using LASCA. To highlight the clinical relevance, this method was ... 3‐fold higher levels of baseline skin microcirculation. LASCA may be applied in the clinic to aid ...
Tópico(s): Acupuncture Treatment Research Studies
2018 - Wiley | Pain Practice
Barbara Ruaro, Alberto Sulli, Carmen Pizzorni, Sabrina Paolino, Vanessa Smith, Maurizio Cutolo,
... BP) values assessed by laser speckle contrast analysis (LASCA) in selected skin areas of hands and face ... volunteer healthy subjects were enrolled after informed consent. LASCA was performed at different areas of the face ( ... those with limited cutaneous SSc (p<0.04).LASCA detects a significant reduction of BP only in ...
Tópico(s): Body Contouring and Surgery
2016 - Elsevier BV | Microvascular Research
Jonathan I. Brauer, Zakari Makama, Vincent Bonifay, Egemen Aydın, Eric D. Kaufman, Iwona B. Beech, Jan Sunner,
Ambient laser ablation and solvent capture by aspiration (LASCA) mass spectrometric imaging was combined with metabolomics high- ... free surfaces demonstrated heterogeneous distributions of corrosion damage. LASCA data were correlated with areas on the coupons ... amounts of corrosion products. However, by using the LASCA imaging interface, images of more than 1000 ions ( ...
Tópico(s): Marine Biology and Environmental Chemistry
2015 - American Institute of Physics | Biointerphases
G.H.S.F.L. Carvalho, Adriana S. Vivacqua, Jano Moreira de Souza, Sergio Palma da Justa Medeiros,
... exposing some decision-making processes, describes a system, LaSca (from Large Scale), to support decisions in large- ... in addition to facilitate the decision-making process, LaSca also allows its users to decide how to ...
Tópico(s): Cognitive Science and Mapping
2011 - Verlag der Technischen Universität Graz | JUCS - Journal of Universal Computer Science