
Alessandra Brunoro Motta, Sônia Regina Fiorim Enumo, Maria Margarida Pereira Rodrigues, Lidiane Leite,
A linguagem tem um papel central na vida humana, contribuindo para o desenvolvimento de habilidades cognitivas e sociais. Entre as formas de avaliar o comportamento lingüístico infantil, as narrativas têm sido empregadas por meio de atividades como descrever figuras e recontar histórias. A avaliação feita por procedimento assistido em que o avaliador auxilia a criança, após uma fase inicial sem ajuda, testando sem auxílio novamente ao final, tem se mostrado apropriada. Investigando este procedimento, ...
Tópico(s): Reading and Literacy Development
2006 - UNIVERSIDADE FEDERAL DO PARANÁ | Interação em Psicologia

Alexandra Cristina Senegaglia, Cármen Lúcia Kuniyoshi Rebelatto, Cláudio Luciano Franck, Juliana Souza Lima, Lidiane Maria Boldrini Leite, Débora Regina Daga, Cleverson Alex Leitão, Patrícia Shigunov, Ana Paula de Azambuja, Elisa Bana, Daniela Boscaro Marsaro, Bruna Schaidt, Andressa Micosky, Valderez Ravaglio Jamur, Yara Schluga, Isadora May Vaz, Lisandro Ribeiro, Alejandro Correa, e Paulo Roberto Slud Brofman,
The coronavirus pandemic is one of the most significant public health events in recent history. Currently, no specific treatment is available. Some drugs and cell-based therapy have been tested as alternatives to decrease the disease's symptoms, length of hospital stay, and mortality. We reported the case of a patient with a severe manifestation of COVID-19 in critical condition who did not respond to the standard procedures used, including six liters of O
Tópico(s): Long-Term Effects of COVID-19
2021 - SAGE Publishing | Cell Transplantation

Letícia Fracaro, Alexandra Cristina Senegaglia, Roberto H. Herai, Amanda Leitolis, Lidiane Maria Boldrini Leite, Cármen Lúcia Kuniyoshi Rebelatto, Paul Travers, Paulo Roberto Slud Brofman, Alejandro Correa,
Mesenchymal stromal cells (MSCs) can self-renew, differentiate into specialised cells and have different embryonic origins—ectodermal for dental pulp-derived MSCs (DPSCs) and mesodermal for adipose tissue-derived MSCs (ADSCs). Data on DPSCs adipogenic differentiation potential and timing vary, and the lack of molecular and genetic information prompted us to gain a better understanding of DPSCs adipogenic differentiation potential and gene expression profile. While DPSCs differentiated readily along ...
Tópico(s): Cancer Cells and Metastasis
2020 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences

Cláudio Luciano Franck, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Sérgio Adriane Bezerra de Moura, Nathália Forti Francisco, Jurandir Marcondes Ribas-Filho,
Burns are lesions in which the thermal energy of the causative agent transfers heat to the surface of the body, causing superficial or deep damage to the skin with protein denaturation in cells and biochemical maladjustments, which delay and disrupt the cicatricial process, increasing the chances of functional and aesthetic sequelae. This study evaluates the influence of adipose tissue-derived stem cells (ADSCs) on burn healing in terms of the size of the cicatricial space and quantified measures ...
Tópico(s): Burn Injury Management and Outcomes
2019 - Hindawi Publishing Corporation | Stem Cells International

Fernanda Cristina Mendes Barussi, Fernanda Zettel Bastos, Lidiane Maria Boldrini Leite, Felipe Yukio Ishikawa Fragoso, Alexandra Cristina Senegaglia, Paulo Roberto Slud Brofman, Anita Nishiyama, Cláudia Turra Pimpão, Pedro Vicente Michelotto,
This research evaluated the effects of bone marrow-derived mononuclear cells (BMMCs) on the inflammatory process in the equine recurrent airway obstruction (RAO). Eight horses in RAO clinical score were divided into cell therapy group (Gcel) treated with a single intratracheal dose of BMMCs, and dexamethasone group (Gdex) treated with 21days of oral dexamethasone. The horses were clinically revaluated on days 7 and 21, together with cytological evaluation of the BALF, and detection of inflammatory markers ( ...
Tópico(s): Immune Response and Inflammation
2016 - Elsevier BV | Respiratory Physiology & Neurobiology

Marcela Ferreira Dias-Netipanyj, Lidiane Maria Boldrini Leite, Edvaldo da Silva Trindade, Andréa Novais Moreno-Amaral, Selene Elífio-Esposito,
Macrophages are cells of high plasticity and can act in different ways to ensure that the appropriate immune response remains controlled. This study shows the effects of the C-type Bothrops jararacussu venom lectin (BJcuL) on the activation of human macrophages derived from the U937 cell line. BJcuL binds on the cell surface, and this event is inhibited by its specific carbohydrate. It induced phagocytosis and production of H2O2, and expression of antigen presentation molecules. It also enhanced ...
Tópico(s): Immune cells in cancer
2016 - Elsevier BV | Toxicology in Vitro

Roberta Targa Stramandinoli‐Zanicotti, André Lopes Carvalho, Cármen Lúcia Kuniyoshi Rebelatto, Laurindo Moacir Sassi, María Fernanda Torres, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Alejandro Correa-Dominguez, Crisciele KULIGOVSKY, Paulo Roberto Slud Brofman,
Stem cell-based regenerative medicine is one of the most intensively researched medical issues. Pre-clinical studies in a large-animal model, especially in swine or miniature pigs, are highly relevant to human applications. Mesenchymal stem cells (MSCs) have been isolated and expanded from different sources. Objective: This study aimed at isolating and characterizing, for the first time, bone marrow-derived MSCs (BM-MSCs) from a Brazilian minipig (BR1). Also, this aimed to validate a new large-animal ...
Tópico(s): Xenotransplantation and immune response
2014 - UNIVERSIDADE DE SÃO PAULO | Journal of Applied Oral Science

Dayane Mayumi Miyasaki, Alexandra Cristina Senegaglia, Sérgio Adriane Bezerra de Moura, Amanda Leitolis, Luiz Guilherme Achcar Capriglione, Letícia Fracaro, Lidiane Maria Boldrini Leite, Paulo Henrique Utumi, Felipe Yukio Ishikawa Fragoso, Fernando Meyer, Alejandro Correa, Paulo Roberto Slud Brofman,
Chronic kidney disease (CKD) is characterized by structural abnormalities and the progressive loss of kidney function. Extracellular vesicles (EVs) from human umbilical cord tissue (hUCT)-derived mesenchymal stem cells (MSCs) and expanded human umbilical cord blood (hUCB)-derived CD133+ cells (eCD133+) maintain the characteristics of the parent cells, providing a new form of cell-free treatment. We evaluated the effects of EVs from hUCT-derived MSCs and hUCB-derived CD133+ cells on rats with CDK induced ...
Tópico(s): Renal and related cancers
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences

Natássia Caroline Resende Corrêa, Crisciele Kuligovski, Ariane Caroline Campos Paschoal, Ana Paula Ressetti Abud, Cármen Lúcia Kuniyoshi Rebelatto, Lidiane Maria Boldrini Leite, Alexandra Cristina Senegaglia, Bruno Dallagiovanna, Alessandra Melo de Aguiar,
With the increasing need to develop in vitro assays to replace animal use, human stem cell-derived methods are emerging and showing outstanding contributions to the toxicological screening of substances. Adult human stem cells such as adipose-derived stem cells (ADSC) and periodontal ligament stem cells (PDLSC) were used as cell substrates for a cytotoxicity assay and toxicity prediction using the neutral red uptake (NRU) assay. First, primary cell cultures from three independent donors, from each ...
Tópico(s): 3D Printing in Biomedical Research
2017 - Elsevier BV | Regulatory Toxicology and Pharmacology

Yasmine Mendes Pupo, Lidiane Maria Boldrini Leite, Alexandra Cristina Senegaglia, Liziane Antunes, Jéssica Mendes Nadal, Eliane Leal de Lara, Rafael Eiji Saito, Sandra Regina Masetto Antunes, William Fernandes Lacerda, Paulo Vítor Farago,
In this study, the preparation and characterization of three hydroxyapatite-based bioactive scaffolds, including hydroxyapatite microspheres (HAps), amoxicillin-hydroxyapatite composite (Amx-HAp), and collagen-hydroxyapatite composite (Col-HAp) were performed. In addition, their behavior in human dental pulp mesenchymal stem cell (hDPSC) culture was investigated. HAps were synthesized through the following methods: microwave hydrothermal, hydrothermal reactor, and precipitation, respectively. hDPSCs ...
Tópico(s): Dental Implant Techniques and Outcomes
2021 - Multidisciplinary Digital Publishing Institute | Materials

Dilson Novais Rocha, Márcio Arêdes Martins, Jimmy Soares, Marcelo Gomes Marçal Vieira Vaz, Maurício de Oliveira Leite, Lidiane Covell, Leonardo Brantes Bacellar Mendes,
The genus Scenedesmus harbors around 120 species, and some strains have been successfully used for mass culture and biotechnological applications. Considering the potential of this genus as a promising feedstock for production of biofuels, mainly biodiesel, it was evaluated the combined effects of trace elements, salinity stress and different cultivation modes (single batch, semi-continuous, and two-stage batch) on lipid productivity of the freshwater strains S. obliquus BR003 and S. bajacalifornicus ...
Tópico(s): Photosynthetic Processes and Mechanisms
2019 - Elsevier BV | Bioresource Technology

Lidiane Pereira de Albuquerque, Leydianne Leite de Siqueira Patriota, Vitor Gonzatto, Emmanuel Viana Pontual, Patrícia Maria Guedes Paiva, Thiago Henrique Napoleão,
Coronaviruses (CoVs) are a broad group of spherical and enveloped viruses that cause diseases in humans and animals. CoVs have become a major threat to public health in the past two decades, exemplified by epidemics of acute respiratory syndromes and, most recently, the coronavirus disease 2019 pandemic. The envelope of CoVs contains spike (S) proteins, which are transmembrane proteins with a crown-like shape involved in cell attachment, cell‒cell fusion, host tropism, and pathogenesis. The receptors ...
Tópico(s): SARS-CoV-2 detection and testing
2020 - Sciencedomain International | Advances in Research

Lidiane Maria Boldrini Leite, Pedro Vicente Michelotto, Sérgio Adriane Bezerra de Moura, Luiz Guilherme Achcar Capriglione, Fernanda Cristina Mendes Barussi, Felipe Yukio Ishikawa Fragoso, Alexandra Cristina Senegaglia, Paulo Roberto Slud Brofman,
Mesenchymal stem cell (MSC) research has demonstrated the potential of these cells to modulate lung inflammatory processes and tissue repair; however, the underlying mechanisms and treatment durability remain unknown. Here, we investigated the therapeutic potential of human bone marrow-derived MSCs in the inflammatory process and pulmonary remodeling of asthmatic BALB/c mice up to 14 d after transplantation. Our study used ovalbumin to induce allergic asthma in male BALB/c mice. MSCs were injected ...
Tópico(s): IL-33, ST2, and ILC Pathways
2020 - SAGE Publishing | Cell Transplantation

Alejandro Correa, Gabriel Salles Ottoboni, Alexandra Cristina Senegaglia, Luiz Guilherme Achcar Capriglione, Nelson Itiro Miyague, Lidiane Maria Boldrini Leite, Valderez Ravaglio Jamur, Cármen Lúcia Kuniyoshi Rebelatto, Márcia Olandoski, Paulo Roberto Slud Brofman,
Pharmacological approaches are partially effective in limiting infarct size. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs) CD133+ have opened new perspectives for the treatment of ischemic areas after infarction. This preclinical study evaluated the effect of intramyocardial transplantation of purified or expanded human umbilical cord blood-derived CD133+ cells on the recovery of rats following acute myocardial infarction (AMI). Histology studies, electrocardiogram, ...
Tópico(s): Mesenchymal stem cell research
2018 - Hindawi Publishing Corporation | Stem Cells International

Lidiane Pereira de Albuquerque, Ana Vitória Leite Monte, Regina Maria Sousa de Araújo,
Objetivo: Destacar, através de uma revisão narrativa, o manejo e medidas de controle da COVID-19 para as gestantes e os possíveis riscos durante a transmissão vertical e a amamentação. Utilizaram-se bases de dados PubMed e Science Direct que retrataram artigos sobre infecções virais, COVID-19, gestação e amamentação. Revisão bibliográfica: Alterações fisiológicas, metabólicas e vasculares podem agravar a apresentação clínica da COVID-19, promovendo disfunção endotelial, ativação do sistema complemento ...
Tópico(s): Maternal and Neonatal Healthcare
2020 - Revista Eletronica Acervo Saude | Revista Eletrônica Acervo Saúde

Javier Jerez‐Roig, Nayara Priscila Dantas de Oliveira, Bartolomeu Fagundes de Lima Filho, Maria Amanda de Farias Bezerra, Monayane Grazielly Leite Matias, Lidiane Macedo Ferreira, Fabienne Louise Juvêncio dos Santos Amaral, Dyego Leandro Bezerra de Souza, Kênio Costa de Lima,
Background/Study Context: Depression is a common psychiatric disorder in the elderly that leads to a decrease in quality of life and functional impairment, among other health problems. The study of depressive symptoms in institutionalized elderly is scarce in Latin America and can contribute to plan prevention and treatment actions in order to improve health conditions for the residents as well as quality of life. Therefore, the aim of this study is to determine the prevalence of depressive symptoms ...
Tópico(s): Aging and Gerontology Research
2016 - Routledge | Experimental Aging Research

Jean Pierre Barros Thibaut, Lidiane Mota Monteiro, Lydia Christina Calcanho Leite, Carla Maria Souza Menezes, Lı́dia Moreira Lima, François Noël,
Schistosomiasis is one of the most prevalent infectious diseases worldwide and classified as a neglected disease for which there is an urgent need for searching new drug candidates. According to TDR/WHO, existing leads with proven schistosomicidal activity, like meclonazepam, might be the objects of further exploration. Here, we decided to investigate if the benzodiazepine binding sites that we recently characterized in adult Schistosoma mansoni could represent the molecular target of meclonazepam ...
Tópico(s): Malaria Research and Control
2009 - Elsevier BV | European Journal of Pharmacology
Thalia Lima Nogueira, Shirley Daiane Alves da Silva, Lidiane Hilário Da Silva, Maria Vitória Silva Leite, José Filipe Alves Da Rocha, Raul Sousa Andreza,
Por meio da sua facilidade de transmissão e altas taxas de mortalidade, o SARS-CoV-2 até 2021 já infectou mais de 15 milhões de brasileiros. Além do período de incubação e manifestação dos sinais clínicos, a COVID-19 acarreta em muito mais sofrimento pela permanência de sequelas que retardam esse quadro, causando infecção multissistêmica pelo acometimento de tecidos vitais como o cardiológico, muscular, neurológico e psicológico. Sendo necessário o manejo por meio da reabilitação que tem por finalidade ...
Tópico(s): Healthcare Regulation
2021 - | Journal Archives of Health

Mariana Macedo Alvim, Lidiane Ayres da Silva, Isabel Cristina Gonçalves Leite, Marcelo Silva Silvério,
Eventos adversos por interações medicamentosas potenciais em unidade de terapia intensiva de um hospital de ensino ARTIGO ORIGINAL INTRODUÇÃOEventos adversos a medicamentos (EAM) são considerados de interesse mundial para os formuladores de políticas públicas, os profissionais de saúde e a população, pois possuem elevada frequência e aumentam a morbimortalidade dos pacientes, constituindo um problema de saúde pública. (1,2)As complicações relacionadas ao uso de medicamentos são o tipo de evento adverso ...
Tópico(s): Patient Safety and Medication Errors
2015 - Associação de Medicina Intensiva Brasileira | Revista Brasileira de Terapia Intensiva

Cármen Lúcia Kuniyoshi Rebelatto, Lidiane Maria Boldrini Leite, Débora Regina Daga, Daniela Boscaro Marsaro, Isadora May Vaz, Valderez Ravaglio Jamur, Alessandra Melo de Aguiar, Thalita Bastida Vieira, Bianca Polak Furman, Cecília Oliveira Aguiar, Paulo Roberto Slud Brofman,
Mesenchymal stromal cells (MSCs) have been considered a therapeutic strategy in regenerative medicine because of their regenerative and immunomodulatory properties. The translation of MSC-based products has some challenges, such as regulatory and scientific issues. Quality control should be standardized and optimized to guarantee the reproducibility, safety, and efficacy of MSC-based products to be administered to patients. The aim of this study was to develop MSC-based products for use in clinical ...
Tópico(s): Cancer Cells and Metastasis
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences

Lidiane Leite, Elizabeth Maria Andrade Aragão,
Este artigo discute a construção do sujeito ético-político a partir das experiências relacionais engendradas nos espaços de um fórum popular em Vitória -ES. Recorre, principalmente, ao pensamento de Michel Foucault acerca do poder e da ética, de modo a problematizar a idéia de sujeito político atrelada, basicamente, à idéia de sujeito de direito. Propõe pensar a experiência política situada na trama articulada entre o poder e a resistência, tomada nos termos éticos do cuidado de si
Tópico(s): Foucault, Power, and Ethics
2010 - UNIVERSIDADE FEDERAL FLUMINENSE | Fractal Revista de Psicologia

Raul Cremonezi Piva, Maria Helena Verdan, Lidiane Schultz Branquinho, Cândida Aparecida Leite Kassuya, Cláudia Andréa Lima Cardoso,
The population uses the aqueous extract as tea from leaves of Ocimum selloi Benth. (alfavaca) for pain and inflammation issues. This study is motivated by a lack of data about inflammation properties of O. selloi.This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition ...
Tópico(s): Ethnobotanical and Medicinal Plants Studies
2021 - Elsevier BV | Journal of Ethnopharmacology

André Faro, Priscilla Maria Habib Mendonça, Lidiane dos Anjos Santos, Naiara Franca Silva, Juliana Karine Leite Tavares,
A presente pesquisa objetivou delinear os principais conceitos acerca dos anabolizantes entre os praticantes de musculação nas academias de Aracaju (SE). Pretendeu-se, também, conhecer a prevalência da utilização destas substâncias na amostra selecionada. A amostra constituiu-se de 58 indivíduos do sexo masculino, entre 18 e 35 anos de idade, com predominância de formação de nível médio e superior. Como resultados, detectou-se que a concepção prevalente quanto aos anabolizantes e aos seus malefícios ...
Tópico(s): Physical Education and Sports Studies
2006 - UNIVERSIDADE ESTADUAL DE MARINGÁ | Psicologia em Estudo

Lidiane Silva Dutra, Magda Narciso Leite, Marcos Antônio Fernandes Brandão, Priscila Aparecida de Almeida, Fernando Antônio Simas Vaz, Marcone Augusto Leal de Oliveira,
Seeds of Aesculus hippocastanum L. are used in European phytotherapy to treat inflammatory and vascular problems, and also to help in the regulation of the microcirculation. Thus, the quality control of herbal medicines using this species is important.To develop and to optimise a capillary zone electrophoresis method to determine total β-escin in different extracts of A. hippocastanum L.The optimal condition found through chemometric approach was: 25 mmol/L of bicarbonate-carbonate buffer, pH 10. ...
Tópico(s): Mycotoxins in Agriculture and Food
2013 - Wiley | Phytochemical Analysis

Bernardo Zoehler, Letícia Fracaro, Lidiane Maria Boldrini Leite, José Samuel da Silva, Paul Travers, Paulo Roberto Slud Brofman, Maria da Graça Bicalho, Alexandra Cristina Senegaglia,
Mesenchymal stromal cells (MSCs) have been used in immunosuppressive therapy due to their therapeutic effects, with the HLA-G molecule seeming to play a fundamental role. This work evaluated alternative MSC sources to bone marrow (BM), namely, umbilical cord tissue (UC), adipose tissue (AD) and dental pulp tissue (DP), and the influence of interferon-γ (IFN-γ) and hypoxia on the cultivation of these cells for use in immunosuppression therapies. Expression of costimulatory markers CD40, CD80 and CD86 and ...
Tópico(s): Wound Healing and Treatments
2022 - Multidisciplinary Digital Publishing Institute | Cells

Frederico C. Martinho, Fábio Renato Manzolli Leite, Rodrigo Arruda‐Vasconcelos, Lidiane Mendes Louzada, Richard P. Darveau, Brenda Paula Figueiredo de Almeida Gomes,
Abstract Introduction We verified the association between selected bacterial profiles and levels of cytokines, chemokines, and the expression of signs and symptoms of primary endodontic infection with apical periodontitis. Methods Samples were collected from 21 root canals, and macrophages were stimulated for 24 hours. Tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-10, IL-12p70, interferon gamma, and chemokine (C-C motif) ligand 2 (CCL2) were measured using cytometric bead array. We investigated ...
Tópico(s): Inflammatory mediators and NSAID effects
2021 - Elsevier BV | Journal of Endodontics

Paulo Souza‐Fonseca‐Guimaraes, Fernando Guimarães, Caroline Souza-Araújo, Lidiane Maria Boldrini Leite, Alexandra Cristina Senegaglia, Anita Nishiyama, Fernando Souza‐Fonseca‐Guimaraes,
Sepsis is a complex systemic inflammatory syndrome, the most common cause of which is attributed to systemic underlying bacterial infection. The complete mechanisms of the dynamic pro- and anti-inflammatory processes underlying the pathophysiology of sepsis remain poorly understood. Natural killer (NK) cells play a crucial role in the pathophysiology of sepsis, leading to exaggerated inflammation due their rapid response and production of pro-inflammatory cytokines such as interferon gamma (IFN-γ). ...
Tópico(s): Immune cells in cancer
2017 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Ismael Inacio Cardoso Antonio, Zanin Kronka Adriana, Brito Lima Lanna Natalia, Priscilla Nátaly de Lima Silva, Fernandes Colombari Lidiane, Leite dos Santos Paula, Geraldi Pierozzi Caroline,
Chemical treatment is known to effectively eradicate or at least reduce the presence of fungi in seeds. However, this treatment should not impair seed quality. The purpose of the present study was to evaluate the physiological and sanitary quality of Cozumel hybrid melon seeds treated with the fungicide Thiabendazole. The experiments were conducted under a completely randomized design, consisting of 15 treatments in a 3×5 factorial system (three seed lots × five Thiabendazole doses: 0, 0.12, 0.2, 0. ...
Tópico(s): Nematode management and characterization studies
2015 - Academic Journals | African Journal of Agricultural Research

Taline Baganha Stefanello Catelan, Joyce Alencar Santos Radai, Maicon Matos Leitão, Lidiane Schultz Branquinho, Paulo César de Paula Vasconcelos, Silvia Cristina Heredia Vieira, Cândida Aparecida Leite Kassuya, Cláudia Andréa Lima Cardoso,
Some species of Campomanesia are used in the folk medicine due to anti-inflammatory, anti-diarrheal, anti-diabetes and hypercholesterolemic. However studies with Campomanesia guazumifolia (Cambess.) O. Berg. are scarce.This study investigated the anti-inflammatory activity and toxicological profile of infusion obtained from leaves of Campomanesia guazumifolia in mice.Leaves infusion of C. guazumifolia was obtained in the proportion of 20 g/L (leaves/water) at 95-100 °C for 10 min in an enclosed container. ...
Tópico(s): Phytochemistry and biological activity of medicinal plants
2018 - Elsevier BV | Journal of Ethnopharmacology

Alexandra Cristina Senegaglia,
Lidiane Maria Boldrini Leite*1, Pedro Vicente MichelottoJr2, Sérgio Adriane Bezerra de Moura3, Paulo Roberto Slud Brofman1 and Alexandra Cristina ... Senegaglia, Core for Cell Technology, Curitiba, Paraná, Brazil Lidiane Maria Boldrini Leite, Core for Cell Technology, Curitiba, Paraná, Brazil DOI: ...
Tópico(s): Tracheal and airway disorders
2019 - Biomedical Research Network | Biomedical Journal of Scientific & Technical Research