Charles S. Millet, E. H. Hayward,
... Contents, Ligature of the Pancreatic Ducts in Dogs, Lipase in Intestinal Juice, The Use of Cool, Warm, ...
1904 - Gale Group | NCCO-STM 1of2
A lipase-catalysed condensation of fatty acid and hydroxylamine is described. Reaction in liver extracts follows the inhibition pattern of liver lipase, hexyl resorcinol and fluoride acting as powerful inhibitors. ... found with pancreatine. The condensation with hydroxylamine on lipase occurs only with relatively high concentrations of hydroxylamine ... length optimun is found with octanoate, while pancreas lipase reacts little with compounds containing below 8 carbons, ... acide gras et d'hydroxylamine catalysée par une lipase. La réaction dans les extraits de foie suit ...
Tópico(s): Mass Spectrometry Techniques and Applications
1950 - Elsevier BV | Biochimica et Biophysica Acta
National Library of Medicine (NCBI)
... modification of the Loevenhart method for determining serum lipase activity, and the Somogyi2 method for determining serum ... The normal values of serum amylase and serum lipase determined by the methods...Bibliography1. CherryCrandall ISLA: The specificity of pancreatic lipase; its appearance in the blood after pancreatic injury, ... CrossrefGoogle Scholar4. SwanMyers KCHB: Experimental studies of serum lipase in multiple sclerosis, Arch. Neurol. and Psychiat. 38: 288, 1937. CrossrefGoogle Scholar5. JergesenSimonds FHJP: Blood lipase in patients with peptic ulcers; its relation to ...
Tópico(s): Pancreatitis Pathology and Treatment
1951 - American College of Physicians | Annals of Internal Medicine
The conditions necessary for optimum lipase activity vary with the source of enzyme, the substrate and the methods used in obtaining the lipase preparation.Thus, it has been reported (4) that ... niger and Penicillium roqueforti produce two types of lipase.The extracellular lipases were most active at pH 8.0, whereas the mycelial lipases exhibited maximum activity at pH 6.5.In ... Tammisto (13) demonstrated that the heat-resistant intracellular lipase of B. jl1w1'escens liquefaciens became heat sensitive ...
Tópico(s): Enzyme Catalysis and Immobilization
1952 - Elsevier BV | Journal of Dairy Science
The conditions necessary for optimum microbial lipase activity vary with the source of the enzyme, the substrate, and the treatment accorded the lipase preparation. Similarly, it appears that the production of bacterial and fungal lipases is related directly to the nutritional and physical ... Nelson (3) observed with Candida lipolytica that low lipase activity was associated with conditions favorable for rapid growth, whereas high lipase activity was associated with less favorable conditions. In ... agar was due to the inactivation of the lipase by acids developed in the glycerol fermentation. This ...
Tópico(s): Pharmacology and Obesity Treatment
1953 - Elsevier BV | Journal of Dairy Science
Merlin H. Peterson, Marvin J. Johnson, Walter V. Price,
... as butyric acid by the action of milk lipase on natural milk fats.It first was suggested by Rice and Markley (18) that milk lipase was one of the causes of rancidity in ... produced experimentally by the addition of sources of lipase, such as rennet paste (11) and pancreatin (13), ... raw skim milk as a means of milk lipase activation (12).Hood et al. (10) confirmed the ... carried out on the basis of the milk lipase theory.A large part of this work has been carried out with commercial lipases or crude enzyme preparations as the source of ...
Tópico(s): Animal Diversity and Health Studies
1948 - Elsevier BV | Journal of Dairy Science
John C. Wilcox, W.O. Nelson, W.A. Wood,
The lipases of microorganisms have been studied extensively from the aspects of occurrence in individual species and factors affecting enzyme production and activity.These lipases exhibit different patterns of substrate hydrolysis which can ... the substrate (4,6,13,14).Specifically, the lipases of Penicillium roqueforti and Aspergiltus niger showed distinct ... tricaprylin was used as substrate.The P. roqueforti lipase released butyric and eaprylic acids in the ratio ... whereas the same acids released by A. niger lipase were in a 1:4 ratio (13).The ...
Tópico(s): Biochemical Analysis and Sensing Techniques
1955 - Elsevier BV | Journal of Dairy Science
Daniel P. Schwartz, I.A. Gould, W.J. Harper,
... enzyme system of milk contains more than one lipase.The effect of formaldehyde on milk lipase activity at various pH levels and times of ... expressed concern about the sensitivity of the milk lipase system to added formaldehyde (1,2,5,6, ... literature when formaldehyde has been used in milk lipase studies have not always been in agreement.The ... postulated the possible existence in milk of two lipases, one being sensitive and the other tolerant to ... reported that formaldehyde inhibited the action of milk lipase on tributyrin by 75% at pH 8.5 ...
Tópico(s): Diet, Metabolism, and Disease
1956 - Elsevier BV | Journal of Dairy Science
Th. Förster, C. Jensen, Emily Plath,
To properly study lipase activity in milk it is desirable to control the loss in activity of the enzyme that occurs during the ... by nitrogen ebullition.Editor.A rapid decrease in lipase activity during the first hours of cold storage ... problem to the investigator interested in estimating the lipase content of fresh milk.Peterson et al. (6) ... 10 to 15 hours prior to making their lipase determinations.This practice provided samples in which the lipase activity remained reasonably constant.The values they reported ...
Tópico(s): Diet, Metabolism, and Disease
1956 - Elsevier BV | Journal of Dairy Science
Cow's milk contains at least two lipases that are present in the plasma of warm, freshly drawn milk.One, the membrane lipase, is irreversibly adsorbed on the fat globules as ... late in their lactation.The other one, plasma lipase, is associated with the caseinate system and it ... milk of nmltiple lipolytic enzymes that include both lipase and simple esterases.Since milk contains no simple ... use of esters other than milk fat for lipase deternfination in milk can not be justified (6, ... hydrolysis of milk fat.A study of the lipase system itself in milk is, therefore, of fundamental ...
Tópico(s): Amino Acid Enzymes and Metabolism
1957 - Elsevier BV | Journal of Dairy Science
Marvin M. Nachlas, Robert Kevin Blackburn,
... and calorimetric (4, 5) techniques, the esterase and lipase activities of sera and many tissues have been ... be generally accepted.First, the ideal substrates for lipase appear to be either monoesters or glycerides of ... ability of esterase to hydrolyze naphthyl laurate and lipase to hydrolyze tributyrin.This fact is probably the ... most suited for the differentiation of esterase from lipase because of its inhibitory action on esterase and its accelerating effect on lipase.By utilizing these two facts, a calorimetric method ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
1958 - Elsevier BV | Journal of Biological Chemistry
... LIPA gene affects splicing and causes lysosomal acid lipase deficiencyLysosomal acid lipase deficiency allograft recurrence and liver failure- clinical outcomes of 18 liver transplantation patientsLysosomal Acid Lipase Deficiency: Report of Five Cases across the Age SpectrumLysosomal acid lipase deficiency - underestimated cause of dislipidemia. what's new? ... and hydrolysis: A contribution to sterol homeostasisLysosomal acid lipase deficiency: a form of non-obese fatty liver ... storage disorder: the phenotypic spectrum of lysosomal acid lipase deficiencyThe role of sebelipase alfa in the treatment ...
Tópico(s): Lysosomal Storage Disorders Research
1963 - American College of Physicians | Annals of Internal Medicine
Virendra Kumar Patel, Herbert S. Goldberg, D. C. Blenden,
... S. Goldberg, and Donald Blenden . Characterization of leptospiral lipase. J. Bacteriol. 88: 877–884. 1964.—A technique for leptospiral lipase extraction which yielded a highly active, stable, and concentrated lipase preparation was developed. The chief characteristics of leptospiral lipase were determined and are summarized below. Leptospiral lipase was soluble in water and stable in both ... enzyme was most active. With this substrate, leptospiral lipase was found to display optimal activity at pH ... at 30 C. The Michaelis constant of leptospiral lipase with tributyrin substrate was determined to be 4. ...
Tópico(s): Leptospirosis research and findings
1964 - American Society for Microbiology | Journal of Bacteriology
The storage stability of milk lipase was markedly increased by the addition of glutathione, hydroquinone, and potassium thiocyanate.These results indicate that the loss of lipase activity on aging milk results mainly from oxidative ... The greater effectiveness of glutathione in stabilizing milk lipase may indicate the importance of sulfhydryl groups for this enzyme activity.The heat inactivation of milk lipase followed first-order kinetics.The pH of optimum ... the range of 6.6-7.6.Milk lipase was very sensitive to photo-inactivation and to ...
Tópico(s): Milk Quality and Mastitis in Dairy Cows
1959 - Elsevier BV | Journal of Dairy Science
The acceleration of lipase action in raw milk by homogenization is now generally accepted.This acceleration has been attributed by some to increased surface area afforded the lipase by the breakdown of the fat globules and ... is accumulating which indicates that factors which affect lipase action in normal milk may not have the same effect on lipase activity in the homogenized product.A limited amount ... been found to be effective in greatly inhibiting lipase action in normal unhomogenized milk (9,10,11, ...
Tópico(s): Animal Diversity and Health Studies
1941 - Elsevier BV | Journal of Dairy Science
... mehrere Krankheitsgruppen unterteilen, welche im einzelnen besprochen werden. Lipase-Erhohungen gehen immer auf ein Pankreopathie zuruck. Eine ... mit operativ oder autoptisch gesichertem Pankreaskarzinom war die Lipase-Aktivitat erhoht. Bei acht Patienten mit begrundetem Verdacht ... auch zu einer deutlichen Pankreasparenchymschadigung fuhren. Erwahnenswert sind Lipase-Erhohungen im Laufe einer Hepatitis und bei Niereninsuffizienz. Zwischen Reststickstoffanstieg und Lipase-Erhohungen besteht eine auffallend gute Korrelation. Determination of ...
Tópico(s): Diabetes Management and Research
1965 - Thieme Medical Publishers (Germany) | DMW - Deutsche Medizinische Wochenschrift
Gilbert Benzonana, P. Desnuelle,
... paper was to study the action of pancreatic lipase (glycerol ester hydrolase, EC 3.1.1.3) ... in an heterogeneous medium. It is shown that: Lipase is adsorbed by its emulsified substrate and the ... For the same weight, the same amount of lipase and the same experimental conditions. v becomes higher ... the total volume (A0) of the emulsion. Thus, lipase adsorption may be considered as fully reversible in ... of emulsion, is playing, in the case of lipase, the role normally devoted to the concentration of the substrate. The Km (emulsion) of lipase corresponds to a certain value of this interface ...
Tópico(s): Lipid metabolism and biosynthesis
1965 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
John C. George, C. L. Talesara,
... PhysiologyVolume 60, Issue 1 p. 33-39 Article Lipase activity of the particulate fractions of the pigeon ... mechanism of the hydrolysis of glycerides by pancreatic lipase. Acta Chem. Scand., 7: 557–558. Borgstrom, B. ... and C. R. Park 1960 Properties of intestinal lipase. J. Biol. Chem., 235: 3076, 3078. Drummond, G. ... George, J. C., and P. Thomas Iype 1962 Lipase activity in the developing chick liver. Proc. Soc. ... George, J. C., and P. Thomas Iype 1962 Lipase activity of the particulate fractions of the pigeon ...
Tópico(s): Meat and Animal Product Quality
1962 - Wiley | Journal of Cellular and Comparative Physiology
Robert L. Ory, Aaron M. Altschul,
Lipases are currently classified among hydrolases. They participate in transfer reactions in which molecules other than water ... have ever been found to be hydrolyzed by lipases. The mammalian lipases are classified into three groups—the lipases discharged into the digestive tract by specialized organs, tissue lipases, and milk lipases. Among the digestive lipases, the enzyme synthesized by the acinar cells of ... brain, muscle, arteries, kidney, adipose tissue, and serum. Lipases are widely distributed among bacteria, yeasts, and fungi. ...
Tópico(s): Pancreatic function and diabetes
1962 - Elsevier BV | Biochemical and Biophysical Research Communications
Per Björntorp, Robert H. Furman,
... in the presence of heparin and epinephrine. The lipase activity present in the incubation medium and in ... homogenization was measured, partially purified, and characterized. A lipase was eluted into the incubation medium which was considered to be identical with lipoprotein lipase described by E. D. Korn. It had a ... sulfate, EDTA, and deoxycholate. The yield of this lipase was greater when heparin was present in the ... obtained from fed rather than fasting rats. This lipase was also present in the tissue homogenates. A ...
Tópico(s): Adipose Tissue and Metabolism
1962 - American Physiological Society | American Journal of Physiology-Legacy Content
... to feeding and fasting of heparin-releasable lipoprotein lipase in rat cardiomyocytesThe Journal of Nutritional Biochemistry, Vol. 3, No. 12Effect of ethanol intake on lipoprotein lipase activity in rat heartBiochemical Pharmacology, Vol. 41, No. 12Lipoprotein lipase in rat heart—I. Characterization of tri-, di- and monoacylglycerol lipase activities in post-heparin effluentsComparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol. 94, No. 1Lipoprotein lipase in rat heart—II. influence of apolipoproteins and ...
Tópico(s): Diabetes Management and Research
1967 - American Physiological Society | American Journal of Physiology-Legacy Content
ARTICLESComparison of heparin-released and epinephrine-sensitive lipases in rat adipose tissueSJ Ho, RJ Ho, and HC MengSJ Ho, RJ Ho, and HC MengPublished Online:01 Feb 1967https://doi.org/10. ... Lipid Research, Vol. 46, No. 8Regulation of lipoprotein lipase in different rat tissuesMetabolism, Vol. 37, No. 6Blood ... lipoprotein lipaseIn Vitro, Vol. 19, No. 5Tissue lipoprotein lipase activity and its action in lipoprotein metabolismInternational Journal ... inhibitors on the synthesis and release of lipoprotein lipase in cultured cells derived from the stromal-vascular ...
Tópico(s): Pharmacology and Obesity Treatment
1967 - American Physiological Society | American Journal of Physiology-Legacy Content
... 2008 | Drug Metabolism Reviews, Vol. 5, No. 2Lipoprotein Lipase in the Fat Body of Triatoma brasiliensis27 September ... Physiologie et de Biochimie, Vol. 84, No. 1Triglyceride lipase activity in subcellular fractions from beef heart18 October ... Letters, Vol. 35, No. 2The Activity of Lipoprotein Lipase in Rat Heart after Tourniquet StressMechanisms of Enzyme and Substrate Activation by Lipoprotein Lipase CofactorsJournal of Biological Chemistry, Vol. 247, No. 8Separation of molecular species of lipoprotein lipase from adipose tissueJournal of Lipid Research, Vol. 13, ...
Tópico(s): Adipokines, Inflammation, and Metabolic Diseases
1966 - American Physiological Society | American Journal of Physiology-Legacy Content
Margit Hamosh, Theresa R. Clary, Sidney S. Chernick, Robert O. Scow,
... The effect of pregnancy and lactation on lipoprotein lipase activity in adipose and mammary tissues and on ... below 1.0 mM throughout lactation. 3. Lipoprotein lipase activity in parametrial adipose tissue, 12 units/g ... the next 7 days, (1 unit of lipoprotein lipase activity = 1 μmole of triglyceride hydrolyzed per h.) ... below 2 units/g throughout lactation. 4. Lipoprotein lipase activity in the inguinal-abdominal mammary glands increased ... parturition to more than 40 units/g. Lipoprotein lipase activity in mammary gland remained high as long ...
Tópico(s): Lipid metabolism and disorders
1970 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Abstract The regulation of lipoprotein lipase activity was studied in rat adipocytes. Incubation of fat cells for 60 min in buffer without glucose and insulin resulted in a 50% decrease in lipoprotein lipase activity. This decrease was prevented by glucose and ... insulin and caused a rapid decay of lipoprotein lipase activity (T½ = 24 min). Lipolytic concentrations of N6, ... of glucose and insulin decreased fat cell lipoprotein lipase activity and also decreased the rate of l- ... rate of protein synthesis and changes in lipoprotein lipase activity caused by lipolytic agents, cycloheximide, and by ...
Tópico(s): Lipid metabolism and biosynthesis
1970 - Elsevier BV | Journal of Biological Chemistry
K. Hayase, Benjamin F. Miller,
... studied in vitro. The tissue contains an acid lipase (pH optimum, 5.4) and an alkaline lipase (pH optimum, 8.8). Both lipases catalyze the hydrolysis of saturated triglycerides; the rate ... and trilinolenate are hydrolyzed at similar rates. Alkaline lipase is inhibited about 50% at 7.2 mm glycerol trioleate, while acid lipase is unaffected at this concentration. Both lipases are activated by Ca(++) ions. The acid lipase is easily inactivated by deionized water used either as a homogenizing or dialyzing medium. Acid lipase is strongly inhibited by BSA, sodium deoxycholate, and ...
Tópico(s): Diabetes, Cardiovascular Risks, and Lipoproteins
1970 - Elsevier BV | Journal of Lipid Research
LaRosa Jc, R I Levy, W. Virgil Brown, Fredrickson Ds,
... Diseases, Vol. 25, No. 4Abnormal activation of lipoprotein lipase by non-equilibrating apoC-II: further evidence for ... 81, No. 6Lipoprotein ApoC-II activation of lipoprotein lipase. Modulation by apolipoprotein A-IV.Journal of Biological ... triacylglycerol hydrolysis in HDL as induced by hepatic lipase: modulation of the phospholipase activity by changes in ... very low density lipoproteins by human milk lipoprotein lipase: The identification of lipoprotein B as the main ... 348, No. 1 Lipoprotein STriglyceride Hydrolysis by Lipoprotein Lipase Bound to Endothelial Cells in CultureHigh-Density Lipoprotein ...
Tópico(s): Lipid metabolism and biosynthesis
1971 - American Physiological Society | American Journal of Physiology-Legacy Content
R. Verger, Louis Sarda, P. Desnuelle,
Porcine pancreatic lipase contains two SH groups (SH1 and SH11). The SH1 group is reactive in the native enzyme towards sulfhydryl ... the preparation of two well-defined S-substituted lipase derivatives: mono 5-thio-2-nitrobenzoic acid (TNB) lipase in which the SH1 group was selectively blocked by a TNB radical and diphenyl mercuric (DPM) lipase in which the SH1 and SH11 groups were ... also be prepared by incubation of mono TNB-lipase with phenylmercuric chloride. All these reactions were found ... reaction was observed to occur when mono TNB-lipase was treated with 0.3% sodium dodecyl sulfate ...
Tópico(s): Hormonal and reproductive studies
1971 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics