Produção Nacional
Roberta Sitnik, Alexandre R. Marra, Roberta Cardoso Petroni, Ozires Ramos, Marinês Dalla Valle Martino, Jacyr Pasternak, Oscar Fernando Pavão dos Santos, Cristóvão Luís Pitangueira Mangueira, João Renato Rebello Pinho,
Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2014 - Instituto Israelita de Ensino e Pesquisa Albert Einstein | Einstein (São Paulo)
Produção Nacional
Revisado por pares
Márcio Lassance Martins de Oliveira, Bernardo Rangel Tura, Mauro Meira Leite, Eduardo José Melo dos Santos, Luís Cristóvão Pôrto, Lygia da Veiga Pereira, Antônio Carlos Campos de Carvalho,
Identifying human leukocyte antigen (HLA) haplotype-homozygous donors for the generation of induced pluripotent stem (iPS) cell lines permits the construction of biobanks immunologically compatible with significant numbers of individuals for use in therapy. However, two questions must be addressed to create such a bank: how many cell lines are necessary to match most of the recipient population and how many people should be tested to find these donors? In Japan and the UK, 50 and 100 distinct HLA- ...
Tópico(s): CAR-T cell therapy research
2023 - Elsevier BV | Stem Cell Reports
Produção Nacional
Revisado por pares
Akinori Cardozo Nagato, Frank Silva Bezerra, Manuella Lanzetti, Alan Aguiar Lopes, Marco Aurélio dos Santos Silva, Luís Cristóvão Pôrto, Samuel Santos Valença,
Summary In this study our aim was to investigate the time courses of inflammation, oxidative stress and tissue damage after hyperoxia in the mouse lung. Groups of BALB/c mice were exposed to 100% oxygen in a chamber for 12, 24 or 48 h. The controls were subjected to normoxia. The results showed that IL‐6 increased progressively after 12 ( P < 0.001) and 24 h ( P < 0.001) of hyperoxia with a reduction at 48 h ( P < 0.01), whereas TNF‐α increased after 24 ( P < 0.001) and 48 h ( P < 0.001). The number of macrophages ...
Tópico(s): High Altitude and Hypoxia
2012 - Wiley | International Journal of Experimental Pathology
Produção Nacional
Revisado por pares
Eduardo Tavares Lima Trajano, Cinthya Sternberg, Mauricio S. Caetano, Marco Aurélio dos Santos Silva, Luís Cristóvão Pôrto, Juliana C. Santos, Marcelo Lima Ribeiro, Clarissa Bichara Magalhães, Walter A. Zin, Cláudia F. Benjamim, Samuel Santos Valença,
The aim of the present study was to investigate the involvement of oxidative stress in acute lung injury (ALI) induced by lipopolysaccharide (LPS) and its effects upon cell structure, function and inflammation. In total, 108 male C57BL/6 mice were divided into seven groups: CTR Group (50 µL of saline) administered intratracheally (i.t.), LPS 6 h (10 µg of LPS − i.t.), LPS 12 h (10 µg of LPS − i.t.), LPS 24 h (10 µg of LPS − i.t.), LPS 48 h (10 µg of LPS − i.t.), LPS 24 h (10 µg − i.t.) + NAC 40 mg/kg (gavage) and 24 ...
Tópico(s): S100 Proteins and Annexins
2011 - Taylor & Francis | Inhalation Toxicology
Produção Nacional
Revisado por pares
Samuel Santos Valença, Carlos Romualdo Rueff-Barroso, Wagner Alves Pimenta, Adriana Correa Melo, Renata Tiscoski Nesi, Marco Aurélio dos Santos Silva, Luís Cristóvão Pôrto,
Nitric oxide (NO) represents one of the most important intra- and extracellular mediators and takes part in both biologic and pathologic processes. This study aimed to verify the treatment with an NO inhibitor and an NO substrate in pulmonary emphysema induced by cigarette smoke (CS) in a murine model. We compared N-acetylcysteine (NAC), a precursor of glutathione, to G-nitro-L-arginine-methyl ester or L-NAME (LN), which is an NO inhibitor, and to l-arginine (LA), which is a substrate for NO formation. ...
Tópico(s): Nitric Oxide and Endothelin Effects
2011 - Elsevier BV | Pulmonary Pharmacology & Therapeutics
Produção Nacional
Alessandra Dellavance, Alexandre Gabriel Júnior, Alice F. U. Cintra, Antônio Carlos Ximenes, Barbara Nuccitelli, Ben Hur Taliberti, Caio Moreira, Carlos Alberto von Mühlen, Carlos David Araújo Bichara, Cláudio Henrique Ramos dos Santos, Cristiane Martinez Yano, Cristóvão Luís Pitangueira Mangueira, Darlene Gonçalves Carvalho, Eloísa Bonfá, Elvira M. Doi, Fabiana Nunes de Carvalho Guimarães, Flávia Ikeda e Araújo, Hugo Mendonça Mundim, Jozélia Rêgo, Lisiane Ericonio dos Anjos Vieira, Luciana Costa Poli, Luís Eduardo Coelho Andrade, Maria Roseli Monteiro Callado, Mauro Meira de Mesquita, Mitiko Sugiyama, Natasha Slhessarenko, Nílzio Antônio da Silva, Orlando Gabriel Carballo, Paulo Guilherme Leser, Paulo Luiz Carvalho Francescantônio, Renata Jarach, Ricardo Machado Xavier, Roger A. Levy, Suzane Pretti Figueiredo Neves, Wilson de Melo Cruvinel, Wilton Silva dos Santos,
OBJETIVO: O Segundo Consenso Brasileiro de Fator Antinuclear (FAN) em Células HEp-2 ratificou os algoritmos de decisão para leitura dos padrões do FAN na imunofluorescência indireta vistos na primeira edição do Consenso Brasileiro, adicionando ainda um novo algoritmo relacionado com os padrões mistos. MÉTODOS: Tendo em vista a habilidade do teste em detectar autoantígenos nos distintos compartimentos celulares, e não apenas no núcleo, propõe-se novas denominações para este exame laboratorial. RESULTADOS ...
Tópico(s): Lymphoma Diagnosis and Treatment
2003 - Elsevier BV | Revista Brasileira de Reumatologia
Produção Nacional
Revisado por pares
Aline Almeida-Oliveira, Monique Smith-Carvalho, Luís Cristóvão Pôrto, Juliana Cardoso-Oliveira, Aline dos Santos Ribeiro, Rosângela Rosa Falcão, Eliana Abdelhay, Luís Fernando Bouzas, Luiz Claudio Santos Thuler, Maria Helena Ornellas, Hilda Rachel Diamond,
Most studies on natural killer (NK) cells and aging have focused on overall cell numbers and global cytotoxic activity. NK cell functions are controlled by surface receptors belonging to three major families: killer cell immunoglobulin-like receptors (KIRs), natural cytotoxicity receptors (NCRs), and C-type lectins. The expression of these receptors was investigated from childhood through old age in T, NKT- and NK cells and also in the CD56dim (cytotoxic) and CD56bright (responsible for cytokine production) ...
Tópico(s): Immune Cell Function and Interaction
2011 - Elsevier BV | Human Immunology
Produção Nacional
Revisado por pares
Jandra Pacheco dos Santos, Ricardo Artigiani Neto, Cristóvão Luís Pitangueira Mangueira, Renée Zon Filippi, Paulo Sampaio Gutierrez, Johanna Westra, Elisabeth Brouwer, Alexandre Wagner Silva de Souza,
Takayasu arteritis (TAK) is a large-vessel granulomatous vasculitis; the inflammatory infiltration in arteries comprises macrophages, multi-nucleated giant cells, CD4+ and CD8+ T cells, γδ T cells, natural killer (NK) cells and neutrophils. However, it is unknown which subtype of macrophages predominates. This study aims to evaluate macrophages subpopulations in the aorta in TAK. Immunohistochemistry was performed in the aorta from TAK patients (n = 22), patients with atherosclerotic disease (n = 9) and ...
Tópico(s): Cell Adhesion Molecules Research
2020 - Oxford University Press | Clinical & Experimental Immunology
Produção Nacional
Alessandra Dellavance, Alexandre Gabriel Júnior, Barbara Nuccitelli, Ben Hur Taliberti, Carlos Alberto von Mühlen, Carlos David Araújo Bichara, Cláudio Henrique Ramos dos Santos, Cleonice Bueno, Cristiane Martinez Yano, Cristóvão Luís Pitangueira Mangueira, Darlene Gonçalves Carvalho, Elizângela Cardoso, Eloísa Bonfá, Flávia Ikeda e Araújo, Gustavo Gabriel Rassi, Hugo Mendonça Mundim, Izidro Bendet, Jozélia Rêgo, Lisiane Maria Enriconi dos Anjos Vieira, Luís Eduardo Coelho Andrade, Maria Ordália Ferro Barbosa, Mitiko Sugiyama, Mittermayer Barreto Santiago, Natasha Slhessarenko, Nílzio Antônio da Silva, Paulo Luiz Carvalho Francescantônio, Renata Jarach, Roberto Suda, Roger A. Levy, Silvia Oliveira Sampaio, Suzane Pretti Figueiredo Neves, Wilson de Melo Cruvinel, Wilton Silva dos Santos, Yanna K. de M. Nóbrega,
OBJETIVO:O 3º Consenso Brasileiro para pesquisa de autoanticorpos em Células HEp-2 (FAN) teve como propósito avaliar as dificuldades de implantação do 2º Consenso ocorrido no ano de 2002, discutir estratégias para controlar a qualidade do ensaio e promover a atualização das associações clínicas dos diversos padrões. MÉTODOS:Participaram do encontro em Goiânia nos dias 13 e 14 de abril de 2008 pesquisadores e especialistas de diversos centros universitários e laboratórios clínicos de diferentes regiões ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2009 - Elsevier BV | Revista Brasileira de Reumatologia
Produção Nacional
Revisado por pares
Guilherme Ramires de Jesús, Marcela Ignacchiti Lacerda, Bruna Costa Rodrigues, Flávia Cunha dos Santos, Adriana P. Nascimento, Luís Cristóvão Pôrto, Nilson Ramires de Jesús, Roger A. Levy, Evandro Mendes Klumb,
Objective To evaluate mean serum levels of vascular endothelial growth factor (VEGF), placental growth factor (PlGF), and soluble Flt‐1 (sFlt‐1) in pregnant patients with systemic lupus erythematosus (SLE) with inactive disease, active lupus nephritis, and preeclampsia for differential diagnosis between these conditions. Methods Pregnant women with SLE, with singleton pregnancies and no other autoimmune diseases, were classified according to disease activity (inactive SLE and active lupus nephritis) ...
Tópico(s): Reproductive System and Pregnancy
2020 - Wiley | Arthritis Care & Research
Produção Nacional
Revisado por pares
Eduardo Tavares Lima Trajano, Larissa Alexsandra da Silva Neto Trajano, Marco Aurélio dos Santos Silva, Neil Grant Venter, Luís Cristóvão Pôrto, Adenilson de Souza da Fonseca, Andréa Monte‐Alto‐Costa,
Tópico(s): Burn Injury Management and Outcomes
2015 - Springer Science+Business Media | Lasers in Medical Science
Produção Nacional
Revisado por pares
Priscilla Bento Matos Cruz Derogis, Lívia Rentas Sanches, Valdir Fernandes de Aranda, Marjorie Paris Colombini, Cristóvão Luís Pitangueira Mangueira, Marcelo Katz, Adriana Caschera Leme Faulhaber, Cláudio Ernesto Albers Mendes, Carlos Eduardo dos Santos Ferreira, Carolina Nunes França, João Carlos de Campos Guerra,
Rivaroxaban is an oral direct factor Xa inhibitor, therapeutically indicated in the treatment of thromboembolic diseases. As other new oral anticoagulants, routine monitoring of rivaroxaban is not necessary, but important in some clinical circumstances. In our study a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was validated to measure rivaroxaban plasmatic concentration. Our method used a simple sample preparation, protein precipitation, and a fast chromatographic ...
Tópico(s): Venous Thromboembolism Diagnosis and Management
2017 - Public Library of Science | PLoS ONE
Alessandra Dellavance, Alexandre Gabriel Júnior, Alice F. U. Cintra, Antônio Carlos Ximenes, Barbara Nuccitelli, Ben Hur Tabilerti, Caio Moreira, Carlos Alberto von Mühlen, Carlos David Araújo Bichara, Cláudio Henrique Radmos dos Santos, Cristiane Martinez Yano, Cristóvão Luís Pitangueira Mangueira, Darlene Gonçalves Carvalho, Eloísa S.D. de O. Bonfá, Elvira M. Doi, Fabiana Nunes de Carvalho Guimarães, Flávia Ikeda e Araújo, Hugo Mendonça Mundim, Jozélia Rêgo, Lisiane Ericonio dos Anjos Vieira, Luciana Poli, Luís Eduardo Coelho Andrade, Maria Roseli Monteiro Callado, Mauro Meira de Mesquita, Mitiko Sugiyama, Natasha Slhessarenko, Nílzio Antônio da Silva, Orlando Gabriel Carballo, Paulo Guilherme Leser, Paulo Luiz Carvalho Francescantônio, Renata Jarach, Ricardo Machado Xavier, Roger A. Levy, Suzane Pretti Figueiredo Neves, Wilson de Melo Cruvinel, Wilton Silva dos Santos,
Tópico(s): Chronic Lymphocytic Leukemia Research
2003 - Elsevier BV | Revista Brasileira de Reumatologia
Produção Nacional
Revisado por pares
Paulo Luiz Carvalho Francescantônio, Luis Eduardo Coelho Andrade, Wilson de Melo Cruvinel, Flávia Ikeda e Araújo, Alessandra Dellavance, Alexandre Gabriel Júnior, Barbara Nuccitelli, Ben Hur Taliberti, Carlos Alberto von Mühlen, Carlos David Araújo Bichara, Cláudio Henrique Ramos dos Santos, Cleonice Bueno, Cristiane Martinez Yano, Cristóvão Luís Pitangueira Mangueira, Darlene Gonçalves Carvalho, Elizângela Cardoso, Eloísa Bonfá, Gustavo Gabriel Rassi, Hugo Mendonça Mundim, Izidro Bendet, Jozélia Rêgo, Lisiane Maria Enriconi dos Anjos Vieira, Maria Ordália Ferro Barbosa, Mitiko Sugiyama, Mittermayer Barreto Santiago, Natasha Slhessarenko, Nílzio Antônio da Silva, Renata Jarach, Roberto Suda, Roger A. Levy, Silvia Oliveira Sampaio, Suzane Pretti Figueiredo Neves, Wilton Silva dos Santos, Yanna K. de M. Nóbrega,
OBJETIVO: O III Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) objetivou discutir estratégias para controlar a qualidade do ensaio, promover a atualização das associações clínicas dos diversos padrões e avaliar as dificuldades de implantação do II Consenso ocorrido no ano de 2002. MÉTODOS: Nos dias 13 e 14 de abril de 2007 participaram do encontro em Goiânia pesquisadores e especialistas de diversos centros universitários e laboratórios clínicos de diferentes regiões do ...
Tópico(s): Immunodeficiency and Autoimmune Disorders
2009 - Sociedade Brasileira de Patologia Clínica | Jornal Brasileiro de Patologia e Medicina Laboratorial
Produção Nacional
Revisado por pares
Luís Cristóvão Pôrto, Gustavo Milson Fabrício-Silva, Bruno Poschetzky, Renata M. Perez, Ronaldo Carneiro dos Santos, Luciana Tricai Cavalini,
Background: The host immune response is an important indicator of the outcome of hepatitis C virus (HCV) infection and disease progression. The aim of this study was to explore cytokine gene polymorphisms as a candidate for susceptibility to persistent HCV infection or HCV spontaneous clearance in a population from Rio de Janeiro, Brazil. Methods: Genetic polymorphisms in the cytokines, tumor necrosis factor-alpha (–308), transforming growth factor-beta 1 (codons 10 and 25), interleukin-10 (IL-10; –1082 ...
Tópico(s): Liver Disease Diagnosis and Treatment
2015 - Dove Medical Press | Hepatic Medicine Evidence and Research
Produção Nacional
Revisado por pares
Marco Aurélio dos Santos Silva, Akinori Cardozo Nagato, Eduardo Tavares Lima Trajano, Jackson Nogueira Alves, Ana Carla Balthar Bandeira, Luís Cristóvão Pôrto, Frank Silva Bezerra,
Smoking plays an important role in cardiovascular diseases. However, the reasons why some individuals develop those diseases and others do not remain to be explained.This study aimed at assessing the redox profile of the heart of different mouse strains after exposure to cigarette smoke.Male mice of the Swiss (n = 10), C3H (n = 10), BALB/c (n = 10) and C57BL/6 (n = 10) strains were exposed to cigarette smoke (12 cigarettes/day), while their respective controls (n = 10) were exposed to ambient air for 60 days. After ...
Tópico(s): Biochemical effects in animals
2013 - Sociedade Brasileira de Cardiologia (SBC) | Arquivos Brasileiros de Cardiologia
Produção Nacional
Revisado por pares
P.M.S.T. de Castro, Helena Nasser, Agessandro Abrahão, Larissa Cardilo dos Reis, Ingred Riça, Samuel Santos Valença, Daniele C. Rezende, Luis Eduardo M. Quintas, Moisés C.M. Cavalcante, Luís Cristóvão Pôrto, Vera Lúcia Gonçalves Koatz,
Neutrophil accumulation response to cigarette smoke (CS) in humans and animal models is believed to play an important role in pathogenesis of many tobacco-related lung diseases. Here we evaluated the lung anti-inflammatory effect of aspirin and indomethacin in mice exposed to CS. C57BL/6 mice were exposed to four cigarettes per day during 4 days and were treated i.p. with aspirin or indomethacin, administered each day 1 h before CS exposure. Twenty four hours after the last exposure, cells and inflammatory ...
Tópico(s): Inflammatory mediators and NSAID effects
2009 - Elsevier BV | Biochemical Pharmacology
Produção Nacional
Revisado por pares
Samuel Santos Valença, Luís Cristóvão Pôrto,
Various studies of emphysema involve long-term exposure of animals to cigarette smoke, focusing on the cell type involved in the protease/antiprotease imbalance and on extracellular matrix degradation. In emphysema, increased expression of metalloproteinases is associated with cytokines, and evidence suggests that the matrix metalloproteinase-12 (MMP-12) plays an important role. Our objective was to investigate tissue inhibitor of metalloproteinase-2 (TIMP-2), tumor necrosis factor-alpha (TNF-alpha) ...
Tópico(s): Air Quality and Health Impacts
2008 - Sociedade Brasileira de Pneumologia e Tisiologia | Jornal Brasileiro de Pneumologia
Produção Nacional
Wilson de Melo Cruvinel, Luís Eduardo Coelho Andrade, Carlos Alberto von Mühlen, Alessandra Dellavance, Antônio Carlos Ximenes, Carlos David Araújo Bichara, Cleonice Bueno, Cristóvão Luís Pitangueira Mangueira, Eloísa Bonfá, Fabiano de Almeida Brito, Fernanda Bull Flumian, Glaucielen Gomes da Silva, Jozélia Rêgo, Lisiane Maria Ericoni dos Anjos, Natasha Slhessarenko, Sandra Gofinet Pasoto, Suzane Pretti Figueiredo Neves, Valéria Valim, Wilton Silva dos Santos, Paulo Luiz Carvalho Francescantônio,
Abstract Background The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations ( www.anapatterns.org ). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2019 - BioMed Central | Advances in Rheumatology
Produção Nacional
Revisado por pares
Romulo Vianna, Giovanna Lopes, Raphaela dos Santos Menezes, Luís Cristóvão Pôrto,
Characterization of three novel HLA-DQB1 alleles: HLA-DQB1*04:02:01:11, -DQB1*05:01:01:13, and -DQB1*06:03:01:04.
Tópico(s): Immune Cell Function and Interaction
2021 - Wiley | HLA
Produção Nacional
Revisado por pares
Thais Oliveira Claizoni dos Santos, Carlos Eduardo dos Santos Ferreira, Cristóvão Luís Pitangueira Mangueira, Adriano Luiz Ammirati, Patrícia Faria Scherer, Marisa Petrucelli Doher, Thais Nemoto Matsui, Bento Fortunato Cardoso dos Santos, Virgílio Gonçalves Pereira, Marcelo C. Batista, Júlio César Martins Monte, Oscar Fernando Pavão dos Santos, Marcelino de Souza Durão,
The use of regional citrate anticoagulation (RCA) in liver failure (LF) patients can lead to citrate accumulation. We aimed to evaluate serum levels of citrate and correlate them with liver function markers and with the Cat/Cai in patients under intensive care and undergoing continuous venovenous hemodiafiltration with regional citrate anticoagulation (CVVHDF-RCA). A prospective cohort study in an intensive care unit was conducted. We compared survival, clinical, laboratorial and dialysis data between ...
Tópico(s): Pancreatitis Pathology and Treatment
2023 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Alessandra P. Lamarca, Luiz Gonzaga Paula de Almeida, Ronaldo da Silva Francisco, Lucymara Fassarella Agnez‐Lima, Kátia Castanho Scortecci, Vinícius Pietta Perez, Otávio J. B. Brustolini, Eduardo Sérgio Soares Sousa, Danielle Secco, Ângela Santos, George Rêgo Albuquerque, Ana Paula Melo Mariano, Bianca Mendes Maciel, Alexandra Lehmkuhl Gerber, Ana Paula de Campos Guimarães, Paulo Ricardo Porfírio do Nascimento, Francisco Paulo Freire Neto, Sandra Rocha Gadelha, Luís Cristóvão Pôrto, Eloiza Helena Campana, Selma M. B. Jerônimo, Ana Tereza Ribeiro de Vasconcelos,
The sharp increase of COVID-19 cases in late 2020 has made Brazil the new epicenter of the ongoing SARS-CoV-2 pandemic. The novel viral lineages P.1 (Variant of Concern Gamma) and P.2, respectively identified in the Brazilian states of Amazonas and Rio de Janeiro, have been associated with potentially higher transmission rates and antibody neutralization escape. In this study, we performed the whole-genome sequencing of 185 samples isolated from three out of the five Brazilian regions, including ...
Tópico(s): Bacteriophages and microbial interactions
2021 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Valdir Fernandes de Aranda, Priscilla Bento Matos Cruz Derogis, Lívia Rentas Sanches, Cristóvão Luís Pitangueira Mangueira, Marcelo Katz, Adriana Caschera Leme Faulhaber, Cláudio Ernesto Albers Mendes, Carlos Eduardo dos Santos Ferreira, Carolina Nunes França, João Carlos de Campos Guerra,
The aim of the study was to evaluate the diagnostic accuracy of thromboelastometry for assessing rivaroxaban concentrations. The accuracy of thromboelastometry was compared with the high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method, which is the gold standard for drug plasma monitoring (the reference standard). Forty-six clinically stable patients were treated with 10, 15, or 20 mg of rivaroxaban once daily (OD group) or 15 mg twice a day (BID group) (no particular indication ...
Tópico(s): Venous Thromboembolism Diagnosis and Management
2019 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Produção Nacional
Revisado por pares
Mauren Fernanda Moller dos Santos, F.C.A.C. Oliveira, Renata Kiyomi Kishimoto, Daniela Borri, Fábio Pires de Souza Santos, Paulo Vidal Campregher, Paulo Augusto Achucarro Silveira, Nelson Hamerschlak, Cristóvão Luís Pitangueira Mangueira, Fernando Barroso Duarte, A.H. Crepaldi, Marco Aurélio Salvino, Elvira Deolinda Rodrigues Pereira Velloso,
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), ...
Tópico(s): Chronic Myeloid Leukemia Treatments
2019 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Produção Nacional
Revisado por pares
Marco Aurélio dos Santos Silva, Karla Maria Pereira Pires, Eduardo Tavares Lima Trajano, Vanessa Martins, Renata Tiscoski Nesi, Cláudia Farias Benjamin, Mauricio S. Caetano, Cinthya Sternberg, Mariana Nascimento Machado, Walter A. Zin, Samuel Santos Valença, Luís Cristóvão Pôrto,
The development of bleomycin-induced pulmonary fibrosis (BLEO-PF) has been associated with differences in genetic background and oxidative stress status. The authors’ aim was to investigate the crosstalk between the redox profile, lung histology, and respiratory function in BLEO-PF in C57BL/6, DBA/2, and BALB/c mice. BLEO-PF was induced with a single intratracheal dose of bleomycin (0.1 U/mouse). Twenty-one days after bleomycin administration, the mortality rate was over 50% in C57BL/6 and 20% in ...
Tópico(s): Occupational and environmental lung diseases
2012 - SAGE Publishing | Toxicologic Pathology
Produção Nacional
Revisado por pares
Cíntia Barros Santos-Rebouças, Rafael Mina Piergiorge, Cristina dos Santos Ferreira, Raquel de Seixas Zeitel, Alexandra Lehmkuhl Gerber, Marta Cristine Félix Rodrigues, Ana Paula de Campos Guimarães, Rodrigo M. Silva, Adriana Rodrigues Fonseca, Rangel Celso Souza, Ana Tereza Antunes Monteiro de Souza, Átila Duque Rossi, Luís Cristóvão Pôrto, Cynthia Chester Cardoso, Ana Tereza Ribeiro de Vasconcelos,
Abstract Background Multisystem Inflammatory Syndrome in Children (MIS-C) is a life-threatening complication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, which manifests as a hyper inflammatory process with multiorgan involvement in predominantly healthy children in the weeks following mild or asymptomatic coronavirus disease 2019 (COVID-19). However, host monogenic predisposing factors to MIS-C remain elusive. Methods Herein, we used whole exome sequencing (WES) on 16 ...
Tópico(s): Inflammasome and immune disorders
2022 - BioMed Central | Molecular Medicine
Produção Nacional
Wilson de Melo Cruvinel, Luís Eduardo Coelho Andrade, Alessandra Dellavance, Antônio Carlos Ximenes, Carlos David Araújo Bichara, Cristóvão Luís Pitangueira Mangueira, Eloísa Bonfá, Fabiano de Almeida Brito, Henrique Ataíde Mariz, Lisiane Maria Enriconi dos Anjos, Sandra Gofinet Pasoto, Valéria Valim, Wilton Ferreira S Santos, Clayson Moura Gomes, Roberpaulo Anacleto Neves, Paulo Luiz Carvalho Francescantônio,
The VI Brazilian Consensus on Autoantibodies against HEp-2 cells for determination of autoantibodies against cellular constituents on HEp-2 cells was held on September, 2019, in Fortaleza (CE, Brazil). The guidelines in this edition were formulated by the group of Brazilian experts discussing the classification of complex patterns, the classification of the nuclear discrete dots (few and multiple), the identification of the discrete fine speckled pattern (AC-4a) and improvements on the ANA report.Sixteen ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2022 - BioMed Central | Advances in Rheumatology
Produção Nacional
Revisado por pares
Eliane Aparecida Rosseto-Welter, Silvia Sanches Rodrigues, Amanda Braga Figueiredo, Carolina Nunes França, Danielle Bruna Leal Oliveira, André Luís Lacerda Bachi, Jônatas Bussador do Amaral, Ricardo Andreotti Siqueira, Laiz Camerão Bento, Ana Paula da Silva, Nydia Strachman Bacal, Carlos Eduardo dos Santos Ferreira, Cristóvão Luís Pitangueira Mangueira, João Renato Rebello Pinho,
Herein, we aimed to follow up on the cellular and humoral immune responses of a group of individuals who initially received the CoronaVac vaccine, followed by a booster with the Pfizer vaccine.Blood samples were collected: before and 30 days after the first CoronaVac dose; 30, 90, and 180 days after the second CoronaVac dose, and also 20 days after the booster with the Pfizer vaccine.Whilst the positivity to gamma interferon-type cellular response increased after the first CoronaVac dose, neutralizing ...
Tópico(s): Retinal and Optic Conditions
2023 - Multidisciplinary Digital Publishing Institute | Vaccines
Produção Nacional
Revisado por pares
Rossana Santiago de Sousa Azulay, Luís Cristóvão Pôrto, Silva Da, Maria da Glória Tavares, Roberta Maria Duailibe Ferreira Reis, Gilvan Cortês Nascimento, Sabrina da Silva Pereira Damianse, Viviane Chaves de Carvalho Rocha, Marcelo Magalhäes, Vandílson Pinheiro Rodrigues, Paulo Ricardo Vilas Boas Carvalho, Manuel dos Santos Faria, Marília Brito Gomes,
Abstract This study aimed to investigate the relationship between genetic ancestry inferred from autosomal and Y chromosome markers and HLA genotypes in patients with Type 1 Diabetes from an admixed Brazilian population. Inference of autosomal ancestry; HLA-DRB1 , -DQA1 and -DQB1 typifications; and Y chromosome analysis were performed. European autosomal ancestry was about 50%, followed by approximately 25% of African and Native American. The European Y chromosome was predominant. The HLA-DRB1*03 and ...
Tópico(s): Digestive system and related health
2021 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Roberta Sitnik, Maira Marranghello Maluf, Ketti Gleyzer de Oliveira, Ricardo Andreotti Siqueira, Carlos Eduardo dos Santos Ferreira, Cristóvão Luís Pitangueira Mangueira, Raymundo Soares Azevedo, Maria Lúcia Gomes Ferraz, Maria Cássia Jacintho Mendes Corrêa, Paulo Roberto Abrão Ferreira, Gérson Fernando Mendes Pereira, Flávia Moreno Alves de Souza, Cristina Pimenta, João Renato Rebello Pinho,
Acute viral hepatitis is a disease of great clinical importance. This study proposes actions to better characterise cases of acute hepatitis in Brazil and to provide relevant information to institutionalised health policies within the Unified Health System. Available data on acute hepatitis in Brazil need to be re-evaluated regarding the different hepatotropic agent (hepatitis A to E virus) frequencies, as well as other agents that can cause similar clinical conditions, such as Herpes Simplex Virus ...
Tópico(s): Mosquito-borne diseases and control
2021 - BMJ | BMJ Open