Ingrid Lekk, Florencia Cabrera-Cabrera, Giorgio Turconi, Jürgen Tuvikene, Eli‐Eelika Esvald, Annika Rähni, Laoise Casserly, Daniel R. Garton, Jaan‐Olle Andressoo, Tõnis Timmusk, Indrek Koppel,
... study, we use three different in vitro approaches—luciferase assay, translating ribosome affinity purification(TRAP), and de ... mRNA translatability in primary neurons, we used dual luciferase reporter assay. Cortical neurons were transfected with firefly luciferase constructs where either Bdnf or control 5′ and ... UTR variants tested displayed a repressive effect on luciferase activity (Fig. S1C), including all most commonly used ... generates an ORF out of frame with the luciferase ORF (Fig. S1, E and F). When analyzing ...
Tópico(s): Nuclear Receptors and Signaling
2023 - Elsevier BV | Journal of Biological Chemistry
Zhenzhen Shi, Yuejing Zhang, Xin Wang, Hailong Pang, Lingyun Jia, Kun Sun, Ji Zhang, Jie Du, Hanqing Feng,
... fluid or the liquid medium is measured by luciferase–luciferin system that is based on the reaction: ATP + luciferase + luciferin → oxyluciferin + AMP + CO 2 + photon emission (Ramachandran ... 2019); (2) due to the high molecular weight, luciferase applied exogenously cannot diffuse across the plasma membrane ( ... the plant, rather than intracellular ATP. After exogenous luciferase is applied, luciferin is supplemented to activate the reaction of ATP with luciferase and luciferin, and the production of photon emission ...
Tópico(s): Cellular transport and secretion
2023 - Wiley | New Phytologist
JY Kim, Josie A. Silvaroli, Gabriela Vásquez Martínez, Bijay Bisunke, A. Luna-Ramírez, Laura A. Jayne, Mei Ji He Ho Feng, Bhavya Girotra, Shirely M. Acosta Martinez, Corynne R. Vermillion, Isaac Z. Karel, Nicholas Ferrell, Noah Weisleder, Sangwoon Chung, John W. Christman, Craig R. Brooks, Sethu M. Madhavan, Kari R. Hoyt, Rachel E. Cianciolo, Anjali A. Satoskar, Diana Zepeda‐Orozco, Jennifer C. Sullivan, Alan J. Davidson, Amandeep Bajwa, Navjotsingh Pabla,
... injured tubular epithelial cells. Chromatin immunoprecipitation and promoter luciferase assays confirmed that ZFP24 bound to a specific ... injured tubular epithelial cells. Chromatin immunoprecipitation and promoter luciferase assays confirmed that ZFP24 bound to a specific ... serum. We used a promoter–reporter lentiviral firefly luciferase vector (Applied Biological Materials, catalog numbers LV653 and ... pairs from the transcription start site) driven firefly luciferase. BUMPT stable cells were then reverse-transfected with ...
Tópico(s): Renal and related cancers
2023 - Elsevier BV | Kidney International
Yanhui Lu, Xiaofeng Huang, Wenping Liang, Yu Li, Mengen Xing, Wenhao Pan, Yun Zhang, Zhe Wang, Weihong Song,
... required for TREM2 expression are largely unknown. By luciferase assay, DNA pull-down, and in silico predictions, ... required for TREM2 expression are largely unknown. By luciferase assay, DNA pull-down, and in silico predictions, ... fragments, were cloned into pGL3-Basic vector for luciferase reporter assay. As in the brain, TREM2 is ... cotransfected the plasmid pCMV-RLuc to express Renilla luciferase under the strong ubiquitous promoter CMV as an ... E) showed nearly zero promoter activity and the luciferase expression under this fragment is even lower than ...
Tópico(s): Alzheimer's disease research and treatments
2023 - Elsevier BV | Journal of Biological Chemistry
Yang Wang, Xinna Ge, Yongning Zhang, Xin Guo, Jun Han, Lei Zhou, Hanchun Yang,
... 11-amino-acid subunit HiBiT derived from NanoLuc luciferase was inserted into the PRRSV genome at four ... 3'-UTR), respectively, and five recombinant viruses with luciferase activity were successfully rescued. The virological characteristics of ... kinetics as the parental virus and keeps the luciferase activity and genetic stability after eight rounds of ... of antiviral reagents by detecting the reduction of luciferase activity, showing a consistent trend with infectious titers, ... virus neutralization assay based on the intensity of luciferase activity. Last, the viral proliferation, virulence, validity, and ...
Tópico(s): Viral gastroenteritis research and epidemiology
2022 - American Society for Microbiology | Microbiology Spectrum
Produção Nacional
Revisado por pares
Vadim R. Viviani, Gabriel F. Pelentir, Vanessa R. Bevilaqua,
Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for ... intracellular biosensors for pH and heavy metals, firefly luciferase pH and metal sensitivities were considered drawbacks in analytical assays. We first demonstrated that firefly luciferases and their pH and metal sensitivities can be ... concentrations through ratiometric analysis. Using Macrolampis sp2 firefly luciferase, the intracellular pH could be ratiometrically estimated in ...
Tópico(s): Biosensors and Analytical Detection
2022 - Multidisciplinary Digital Publishing Institute | Biosensors
Iris te Paske, Arjen R. Mensenkamp, Kornelia Neveling, Nicoline Hoogerbrugge, Marjolijn J. L. Ligtenberg, Richarda M. de Voer, Stéphanie Baert‐Desurmont, Kathleen Claes, Kim De Leeneer, Lisa Elze, Simone van den Heuvel, Rachel S. van der Post, Yvonne van Twuijver, Tjakko J. van Ham, Anja Wagner, Mirjam M. de Jong, Edward M. Leter, Maartje Nielsen,
... duplication in the MLH1 promoter region using a luciferase reporter assay. Individual LLP004 carried 2 deep intronic ... only analyzed by mini-gene analysis or a luciferase reporter assay. Mini-gene analysis of the Alu ... MLH1 c.-404_-357dup) was assessed using a luciferase reporter assay (Supplementary Methods).5Hitchins M.P. et ... the pGL3-Basic-GW vector containing a firefly luciferase cassette by means of Gateway Cloning. Per transfection, ... positive control (c.-27C>A; associated with decreased luciferase activity4Hitchins M.P. et al.Cancer Cell. 2011; ...
Tópico(s): Cancer Genomics and Diagnostics
2022 - Elsevier BV | Gastroenterology
Maxim A. Moroz, Juan Zurita, Anna Moroz, Ekaterina Nikolov, Yury Likar, Konstantin Dobrenkov, Jason T. Lee, Larissa Shenker, Ronald G. Blasberg, Inna Serganova, Vladimir Ponomarev,
... characterized a novel bioluminescence reporter—membrane-anchored Cypridina luciferase (maCLuc)—paired with a unique vargulin substrate. This luciferase-substrate pair has no cross-reactivity with established d-luciferin- or coelenterazine-based luciferase reporters. We compare maCLuc with several established luciferase-based reporter systems (firefly, click beetle, Renilla, and Gaussia luciferases), using both in vitro and in vivo models. ... of these reporter systems, which allow for multiplexed-luciferase imaging of 3 and 4 separate targets concurrently ...
Tópico(s): Cell Image Analysis Techniques
2021 - Elsevier BV | Molecular Therapy — Oncolytics
Maša Kenda, Jan Vegelj, Barbara Herlah, Andrej Perdih, Přemysl Mladěnka, Marija Sollner Dolenc,
Firefly luciferase is susceptible to inhibition and stabilization by compounds under investigation for biological activity and toxicity. This can ... in in vitro cell-based assays. However, firefly luciferase remains one of the most commonly used reporter ... Here, we evaluated isoflavonoids for inhibition of firefly luciferase. These natural compounds are often studied using luciferase reporter-gene assays. We used a quantitative structure– ... concomitant detection of inhibition of firefly and Renilla luciferases. The QSAR model predicted a moderate to high ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
M. Hauge, Jennifer Pham, Helena Mancebo, Asuka Inoue, Wesley B. Asher, Jonathan A. Javitch,
... unmodified receptors. Our strategy uses NanoLuc, an engineered luciferase from Oplophorus gracilirostris (deep-sea shrimp) that is smaller and brighter than other well-established luciferases. Recently, several publications have explored functional NanoLuc split ... unmodified receptors. Our strategy uses NanoLuc, an engineered luciferase from Oplophorus gracilirostris (deep-sea shrimp) that is smaller and brighter than other well-established luciferases. Recently, several publications have explored functional NanoLuc split ...
Tópico(s): Photoreceptor and optogenetics research
2021 - Elsevier BV | Journal of Biological Chemistry
Anna S. Young, Thomas Zoeller, Russ Hauser, Tamarra James‐Todd, Brent A. Coull, Peter Behnisch, Abraham Brouwer, Hongkai Zhu, Kurunthachalam Kannan, Joseph G. Allen,
... with Human Nuclear Hormone Receptors in Cell-Based Luciferase Reporter Assays Anna S. Young, Thomas Zoeller, Russ ... activities of extracted dust in five cell-based luciferase reporter assays and dust concentrations of 42 measured ... field sites on various sampling days.Cell-Based Luciferase Reporter Gene AssaysThe dust samples and field blanks were analyzed in chemically activated luciferase gene expression (CALUX) assays by BioDetection Systems. Based ... did not proceed further (Figure S2bi,bj).These luciferase reporter gene assays employed human female osteosarcoma cell ...
Tópico(s): Indoor Air Quality and Microbial Exposure
2021 - National Institute of Environmental Health Sciences | Environmental Health Perspectives
Yuri Tolkach, Romina Zarbl, Simone Bauer, Manuel Ritter, Jörg Ellinger, Stephan Hauser, Laura Hüser, Sabine M. Klauck, Peter Altevogt, Holger Sültmann, Dimo Dietrich, Glen Kristiansen,
... ERG-inducible LNCaP TMPRSS2:ERG (T2E) cells and luciferase promoter assays were used. DNA methylation of the ... strong induction of CD24 mRNA and protein expression. Luciferase promoter assays using the wild-type and mutated ... ERG-inducible LNCaP TMPRSS2:ERG (T2E) cells and luciferase promoter assays were used. DNA methylation of the ... strong induction of CD24 mRNA and protein expression. Luciferase promoter assays using the wild-type and mutated ... Cloning primers are listed in Table 2. The luciferase assay was performed in LNCaP T2E cells. Briefly, ...
Tópico(s): Cancer-related molecular mechanisms research
2021 - Elsevier BV | American Journal Of Pathology
Marrit Putker, David Wong, Estere Seinkmane, Nina M. Rzechorzek, Aiwei Zeng, Nathaniel P. Hoyle, Johanna E. Chesham, Mathew D. Edwards, Kevin A. Feeney, Robin Fischer, Nicolai Peschel, Ko‐Fan Chen, Michael Vanden Oever, Rachel S. Edgar, Christopher P. Selby, Aziz Sancar, John S. O’Neill,
... using the genetically encoded PER2::LUC clock protein::luciferase fusion reporter (Maywood et al, 2011; Ono et ... D. Normalised, detrended bioluminescence recording of the XLG-luciferase reporter (XLG-LUC; equivalent of PER2::LUC) expressed ... are regulated post-transcriptionally Standard curve of recombinant luciferase that was used to determine the number of PER2::LUC molecules. Known concentrations of recombinant luciferase were spiked into (non-luciferase containing) cell lysate to reproduce experimental conditions, and ...
Tópico(s): Photoreceptor and optogenetics research
2021 - Springer Nature | The EMBO Journal
Mandi Gandelman, Warunee Dansithong, Stephen C. Kales, Sharan Paul, Gentrie Maag, Erika Aoyama, Alexey Zakharov, Ganesha Rai, Thomas S. Dexheimer, Brooke M. Whitehill, Hongmao Sun, Ajit Jadhav, Anton Simeonov, Mark J. Henderson, Duong P. Huynh, Stefan M. Pulst, Daniel R. Scoles,
... a potential therapeutic avenue. Using a cell-based luciferase reporter of SNCA expression we performed a quantitative ... a potential therapeutic avenue. Using a cell-based luciferase reporter of SNCA expression we performed a quantitative ... was generated using genome editing to insert the luciferase gene downstream and in frame of the SNCA ... Previously we engineered a cell line that expresses luciferase from the SNCA locus, HEK-293-SNCA-luc, ... response to SNCA siRNA or a small molecule luciferase inhibitor (19Dansithong W. Paul S. Scoles D.R. ...
Tópico(s): Autophagy in Disease and Therapy
2021 - Elsevier BV | Journal of Biological Chemistry
Sonja Blumenstock, Elena Katharina Schulz‐Trieglaff, Kerstin Voelkl, Anna‐Lena Bolender, Paul Lapios, Jana Lindner, Mark S. Hipp, F. Ulrich Hartl, Rüdiger Klein, Irina Dudanova,
... neuronal proteostasis. The mice express EGFP-fused firefly luciferase (Fluc-EGFP), a conformationally unstable protein that requires ... of intracellular Fluc-EGFP foci and by reduced luciferase activity. Using these mice, we provide evidence for ... and mutated versions of the conformationally unstable firefly luciferase (Fluc) protein fused to EGFP are ideal for ... readouts: decrease in bioluminescence due to decline in luciferase activity and formation of Fluc-EGFP foci in ... two-tailed t-test. Left, quantification of specific luciferase activity of FlucSM-EGFP upon indicated treatments, normalized ...
Tópico(s): Mitochondrial Function and Pathology
2021 - Springer Nature | The EMBO Journal
Weiwei Zheng, Qing Chu, Tianjun Xu,
... miR-217-5p, thereby preventing it from inhibiting luciferase activity (Fig. 3E). Taken together, these data suggest ... miR-217-5p. Then, we constructed the NARL luciferase plasmid and the mutant plasmid with the mutated miR-217-5p binding site (Fig. 4A). The luciferase assay shows that miR-217-5p mimics and ... can both significantly inhibit the wild-type NARL luciferase plasmid activity, but have no effect on the ... IL-8, IL-1β, and IRF3 report genes' luciferase activity by inhibiting NOD1 (Fig. 5C). Furthermore, we ...
Tópico(s): interferon and immune responses
2021 - Elsevier BV | Journal of Biological Chemistry
András Dávid Tóth, Dániel Garger, Susanne Prokop, Eszter Soltész‐Katona, Péter Várnai, András Balla, Gábor Turu, László Hunyady,
... receptor interactions in live cells using the Gaussia luciferase (GLuc) as a bioluminescence resonance energy transfer donor. ... receptor interactions in live cells using the Gaussia luciferase (GLuc) as a bioluminescence resonance energy transfer donor. ... tagging with naturally nonsecreted and high-molecular-weight luciferases (such as Renilla luciferase) induces endoplasmic retention and impaired PM expression of ... been overcome by the development of the small luciferase NanoLuciferase (NanoLuc) (14Hall M.P. Unch J. Binkowski ...
Tópico(s): Molecular Communication and Nanonetworks
2021 - Elsevier BV | Journal of Biological Chemistry
M. Neefjes, Bas Housmans, Guus van den Akker, L.W. van Rhijn, Tim J. M. Welting, P.M. van der Kraan,
... compared the characteristics of five reporter genes (Firefly luciferase, stable- and unstable Nano luciferase, secretable Gaussia luciferase and Red Fluorescent Protein) to study complex body ... a reporter gene and slow kinetics compared to luciferases. Intracellularly measured luciferases, such as Firefly luciferase and Nano luciferase, revealed good compatibility with complex body fluids. Secreted Gaussia luciferase appeared to be incompatible with complex body fluids, ...
Tópico(s): Neurobiology and Insect Physiology Research
2021 - Nature Portfolio | Scientific Reports
Jérôme Delroisse, Laurent Duchatelet, Patrick Flammang, Jérôme Mallefet,
... luciferin substrate, an oxygen derivative, and a specialised luciferase enzyme. In some cases, the enzyme and the ... have indeed been discovered, and several non-homologous luciferases lato sensu have been identified which, all together, ... some phylogenetically related organisms may use non-homologous luciferases (e.g., at least four convergent luciferases are found in Pancrustacea), it has also been observed that phylogenetically distant organisms may use homologous luciferases (e.g., parallel evolution observed in some cnidarians, ...
Tópico(s): bioluminescence and chemiluminescence research
2021 - Frontiers Media | Frontiers in Marine Science
Ryota Kurimoto, Tomoki Chiba, Yoshiaki Ito, Takahide Matsushima, Yuki Yano, Kohei Miyata, Yuka Yashiro, Tsutomu Suzuki, Kozo Tomita, Hiroshi Asahara,
... RNA-binding proteins using a let-7 sensor luciferase reporter and identified the tRNA pseudouridine synthase, TruB1. ... that regulate let-7 miRNA by using a luciferase reporter assay with a set of expression plasmid ... inserting a let-7a target site into the luciferase 3′UTR region driven by the SV-40 promoter in pLuc2 plasmids (Fig 1A). Thus, luciferase activity is repressed in the presence of let7 ... cDNAs in the RBP library, expression of the luciferase reporter would be induced. As a positive control ...
Tópico(s): MicroRNA in disease regulation
2020 - Springer Nature | The EMBO Journal
Hiromi Murata, Susumu Tanaka, Tomoko Tsuzuki‐Nakao, Takeharu Kido, Maiko Kakita‐Kobayashi, Naoko Kida, Yoji Hisamatsu, Hiroaki Tsubokura, Yoshiko Hashimoto, Masaaki Kitada, Hidetaka Okada,
... IL15 gene through in silico analysis. Using a luciferase reporter assay, we found that the upstream region ... IL15 gene through in silico analysis. Using a luciferase reporter assay, we found that the upstream region ... on IL15 transcription in ESCs, we performed a luciferase reporter assay with IL15ups/pGL4.10 (Fig. 4A) ... 35), but E2 and MPA treatment significantly increased luciferase activity of IL15ups/pGL4.10 (6.78 ± 1. ... secretory phase, E2 and MPA stimulation significantly increased luciferase activity (18.79 ± 2.20, p < 0.05; ...
Tópico(s): Endometriosis Research and Treatment
2020 - Elsevier BV | Journal of Biological Chemistry
Xing Liu, Chunchun Zhu, Huangyuan Zha, Jinhua Tang, Fangjing Rong, Xiaoyun Chen, Sijia Fan, Chenxi Xu, Juan Du, Junji Zhu, Jing Wang, Gang Ouyang, Guangqing Yu, Xiaolian Cai, Zhu Chen, Wuhan Xiao,
... effect of SIRT5 on promoter activity. IFNβ promoter luciferase reporter and ISRE-luciferase reporter (containing interferon stimulated response elements) are well- ... IFNβ promoter reporter (IFNβ-luc.) activity and ISRE-luciferase reporter activity were strongly inhibited (Fig 1A–D). ... SeV-induced IFNβ promoter reporter activity and ISRE-luciferase reporter activity were enhanced upon SIRT5 knockdown by ... SeV-induced IFNβ promoter reporter activity and ISRE-luciferase reporter activity were enhanced in SIRT5−/− cells compared ...
Tópico(s): Toxoplasma gondii Research Studies
2020 - Springer Nature | The EMBO Journal
Produção Nacional
Revisado por pares
Mariele C. Carvalho, Atílio Tomazini, Danilo T. Amaral, M.T. Murakami, Vadim R. Viviani,
Firefly luciferases usually emit green-yellow bioluminescence at physiological pH values. However, under acidic conditions, in the presence of ... bioluminescence colors and pH-sensitivity, about 20 firefly luciferases have been cloned, sequenced and investigated. The proton ... responsible for pH- and metal sensitivity in firefly luciferases was shown to involve the residues H310, E311 and E354 in firefly luciferases. However, it is still unclear how and why pH-sensitivity arose and evolved in firefly luciferases. Here, we cloned and characterized two novel luciferase ...
Tópico(s): Cell Image Analysis Techniques
2020 - Springer Science+Business Media | Photochemical & Photobiological Sciences
Siu Chiu Chan, Sachin Hajarnis, Sophia M. Vrba, Vishal Patel, Peter Igarashi,
... a TCF/LEF1-binding site linked to a luciferase reporter gene. Induction of the DN–HNF-1β mutant increased both basal and Wnt3a-stimulated luciferase activity compared with uninduced cells (Fig. 2A). Treatment ... phosphorylation of β-catenin by GSK3β, further increased luciferase activity. Mutation of the TCF/LEF1–binding sites ... FOPFlash reporter plasmid largely abolished the stimulation of luciferase activity (Fig. 2B). The significantly higher luciferase activity in DN-HNF-1β–expressing cells treated ...
Tópico(s): Epigenetics and DNA Methylation
2020 - Elsevier BV | Journal of Biological Chemistry
Ewa Jabłońska, Emilia Białopiotrowicz, Maciej Szydłowski, Monika Prochorec–Sobieszek, Przemysław Juszczyński, Anna Szumera‐Ciećkiewicz,
... Sigma-Aldrich, annealed, and cloned into psiCheck-2 luciferase reporter vector (Promega, Madison, WI). HEK293T cells were ... 2000 (Thermo Fisher). Twenty-four hours after transfection, luciferase activities were determined as previously described [24Sewastianik T ... s instructions. Reporter assays with DEPTOR promoter-driven luciferase vector were performed in RIVA and U2932 cell ... GGGGACTTTCC‐3′) upstream of the minimal promoter driving luciferase expression (Addgene plasmid No. 14886, deposited by David ... served as a reporter construct for NFκB activation. Luciferase activities were measured using the Dual-Luciferase Reporter ...
Tópico(s): Immune Cell Function and Interaction
2020 - Elsevier BV | Experimental Hematology
Gang Du, Yicheng Qiao, Zhangpeng Zhuo, Jiaqi Zhou, Xiaorong Li, Zhiming Liu, Li Yang, Haiyang Chen,
... an octoploid mature enterocyte (ECs; Pdm1+). Quantification of luciferase activity after administration of endogenous chemicals. Error bars ... in Drosophila midguts was tested using an "esg-luciferase" reporter system (Figs 1A and B, and EV1B). This system is based on the luciferase reporter expression by esg-GAL4-driven UAS-luciferase in Esg+ cells, which allows the tracing and ... accumulation in aged midguts (Fig 1B). Moreover, the luciferase activity of aged flies (40 days) in response ... ISCs) A. A model illustrating the Drosophila "esg>luciferase" reporter system of chemical administration. The chemicals were ...
Tópico(s): Biochemical Acid Research Studies
2020 - Springer Nature | EMBO Reports
Seiya Ozono, Yanzhao Zhang, Minoru Tobiume, Satoshi Kishigami, Kenzo Tokunaga,
... HiBiT. This peptide is a fragment of NanoLuc luciferase and generates a strong luminescent signal when complemented ... importantly, by comparing between ELISA and the HiBiT luciferase assay, we successfully obtained an excellent linear correlation between p24 concentrations and HiBiT-based luciferase activity. Overall, we conclude that HiBiT-tagged viruses ... HiBiT. This peptide is a fragment of NanoLuc luciferase and generates a strong luminescent signal when complemented ... importantly, by comparing between ELISA and the HiBiT luciferase assay, we successfully obtained an excellent linear correlation ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2020 - Elsevier BV | Journal of Biological Chemistry
Shungo Nakamura, Nae Fu, Kumiko Kondo, Ken‐ichi Wakabayashi, Toru Hisabori, Kazunori Sugiura,
... the first application of BRET by using Renilla luciferase (Rluc) (14Cormier M.J. Hori K. Anderson J. ... et al. (16Hoshino H. Nakajima Y. Ohmiya Y. Luciferase-YFP fusion tag with enhanced emission for single- ... M.R. Meisenheimer P.L. et al.Engineered luciferase reporter from a deep sea shrimp utilizing a ... PubMed Scopus (664) Google Scholar) is the brightest luciferase derived from the Oplophorus luciferase. Venus is an EYFP derivative, which is 30- ... M.R. Meisenheimer P.L. et al.Engineered luciferase reporter from a deep sea shrimp utilizing a ...
Tópico(s): Photoreceptor and optogenetics research
2020 - Elsevier BV | Journal of Biological Chemistry
Alejandro Sarrion‐Perdigones, Yezabel Gonzalez, Koen J. T. Venken,
... be independently detectable and cover large dynamic ranges. Luciferases are good candidates for generation of such signals. ... be sensitively detected. We recently developed a multiplex luciferase assay that allows monitoring the activity of five ... used synthetic assembly cloning to assemble all six luciferase reporter units into a single vector over eight ... experimental variation. Our proof‐of‐concept multiplex hextuple luciferase assay was designed to simultaneously monitor the p53, ... allows the stitching of numerous other cellular pathway luciferase reporters. Here we present an improved three‐step ...
Tópico(s): Cell Image Analysis Techniques
2020 - Wiley | Current Protocols in Molecular Biology
Eunkyoung Shin, Hanyong Jin, Dae‐Shik Suh, Yongyang Luo, Hye‐Jeong Ha, Tae Heon Kim, Yoonsoo Hahn, Seogang Hyun, Kangseok Lee, Jeehyeon Bae,
... inserted, in-frame, into the CDS of the luciferase gene (Fig 3A). miR-1236 transfection decreased the activity of the luciferase reporter harboring the 402C>G FOXL2 variant sequence by 70%, without affecting the activity of the luciferase reporter containing the WT FOXL2 sequence (Fig 3B). ... were inserted in the 3′-UTR of the luciferase reporter gene (Fig 3C and D). The specificity ... targets 402C>G A. Schematic representation of the luciferase reporter constructs used to assay miR-1236 activity ... inserted in-frame into the CDS of the luciferase gene in the pGL3 control vector. B. Luciferase ...
Tópico(s): Immune Cell Function and Interaction
2020 - Springer Nature | The EMBO Journal