... seems to be very little doubt but that luciferase is a protein or so closely associated with ... has properties which agree completely with those of luciferase. Dubois believes Pholas luciferase to be an oxidizing enzyme similar to the ... alcohol, etc. He has detected iron in a luciferase solution which has dialyzed against running water for ... and to act as an "oxyzymase ferrique." Cypridina luciferase, on the other hand, is not readily destroyed ... use of them for this purpose, keeping the luciferase solutions for many months. Professor A. H. Phillips ...
Tópico(s): Molecular Communication and Nanonetworks
1919 - Rockefeller University Press | The Journal of General Physiology
Milton J. Cormier, John R. Totter, H. H. Rostorfer,
Various bacterial luciferase preparations have been compared for their ability to oxidize DPNH in the presence of quinones and ferricyanide and ... production. From such studies it was concluded that luciferase is not a DPNH oxidase and that the ... light production. Purification of five different types of luciferase preparations by starch-column electrophoresis resulted in an ... oxidase obtained from E. coli was added. Some luciferase preparations from A. fischeri showed an absolute requirement ... Apparently, the nature of the activation of purified luciferase by these compounds lies, for the most part, ...
Tópico(s): Photoreceptor and optogenetics research
1956 - Elsevier BV | Archives of Biochemistry and Biophysics
Ann Gunsalus-Miguel, Edward A. Meighen, M. Ziegler Nicoli, Kenneth H. Nealson, J. Woodland Hastings,
... isolation and purification on a large scale of luciferases from two different bioluminescent bacteria, Pf and MAV. In only three major steps luciferase can be obtained in greater than 95% purity. ... the subunits with the removal of urea, renatured luciferase with a high specific activity is obtained. These procedures should be generally applicable with luciferases from different bacterial strains, including mutant luciferases. The two luciferases have been used to produce specific antiluciferases, which, ...
Tópico(s): Photoreceptor and optogenetics research
1972 - Elsevier BV | Journal of Biological Chemistry
Shiao‐Chun Tu, Cory A. Waters, J. Woodland Hastings,
Properties of photoexcitable luciferase are compared with those of luciferase, both isolated from the bacterium Beneckea harveyi. The proteins have the same molecular ... with comparable efficiencies, by antibodies against either pure luciferase (a heterodimeric protein) or individual subunits thereof. Compared with luciferase, photoexcitable luciferase has a broader pH range for optimal activity, ... chromophore, designated B, can be isolated from photoexcitable luciferase. The binding of B to luciferase restores all ...
Tópico(s): 3D Printing in Biomedical Research
1975 - American Chemical Society | Biochemistry
Thomas F. Holzman, Thomas Baldwin,
Upon limited proteolysis of luciferases from the luminous marine bacteria Photobacterium fischeri, Photobacterium phosphoreum, and Beneckea harveyi, the rate of loss of luciferase activity is the same as the rate of ... thus appears that the larger subunit of the luciferase from P. phosphoreum should be designated alpha based ... apparent homology with the alpha subunits of the luciferases from B. harveyi and P. fischeri. The luciferase from B. harveyi is more sensitive to chymotrypsin than to trypsin; the luciferases of the Photobacterium species are more sensitive to ...
Tópico(s): Microbial Community Ecology and Physiology
1980 - National Academy of Sciences | Proceedings of the National Academy of Sciences
... in single barnacle muscle fibres by microinjections DuPont luciferase-luciferin. 2. Injection of DuPont luciferase-luciferin into a cannulated fibre results almost immediately ... reaction mixture. 3. (i) Injection of dehydroluciferin before luciferase-luciferin reduces peak flash in a dose-dependent ... medium (10 mM-HCO3-ASW) shortly before injecting luciferase-luciferin results in a reduction in peak flash. ... 8. 5. Injection of pyrophosphate (PPi) shortly after luciferase-luciferin causes a dual effect: PPi in low ...
Tópico(s): Enzyme function and inhibition
1980 - Wiley | The Journal of Physiology
A.M. Michelson, M.F. Isambert,
Pholad luciferase has been obtained completely pure. It is a high molecular weight (310,000) ferroprotein containing four atoms ... superoxide dismutase does not inhibit the bioluminescent reaction luciferase/O2/luciferin but at higher pH inhibition is ... O2/phosphate. A mechanism is proposed in which luciferase and O2 produce enzyme bound superoxide radical with ... rise to light emission. Stimulation of the enzymic (luciferase) oxidation of luciferin is observed in presence of ... or of O2⨪ or by induction of a luciferase modulated and controlled free radical chain reaction. La ...
Tópico(s): Advanced Chemical Sensor Technologies
1973 - Elsevier BV | Biochimie
The luciferase of the luminous marine bacterium Vibrio harveyi is rapidly inactivated during late log phase and stationary phase. ... synthesis and degradation of total protein and of luciferase throughout growth of the culture shows that the ... low level in stationary phase.The synthesis of luciferase was maximal in early log phase and declined ... to near zero at about the time that luciferase inactivation began.Luciferase was stable throughout exponential growth and accumulated in the cells, but during stationary phase, luciferase cross-reacting material was lost from the 100, ...
Tópico(s): Advanced Chemical Sensor Technologies
1982 - Elsevier BV | Journal of Biological Chemistry
Jeffrey R. de Wet, Keith V. Wood, M. DeLuca, Donald R. Helinski, Suresh Subramani,
The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. The gene contains six introns, ... bases in length. The 5' end of the luciferase mRNA was determined by both S1 nuclease analysis and primer extension. Although the luciferase cDNA clone lacked the six N-terminal codons ... the missing 5' sequence. The full-length, intronless luciferase gene was inserted into mammalian expression vectors and ... CV-1) cells in which enzymatically active firefly luciferase was transiently expressed. In addition, cell lines stably ...
Tópico(s): Bacillus and Francisella bacterial research
1987 - Taylor & Francis | Molecular and Cellular Biology
Jeffrey R. de Wet, Keith V. Wood, Marlene DeLuca, Donald R. Helinski, Suresh Subramani,
The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. The gene contains six introns, ... bases in length. The 5' end of the luciferase mRNA was determined by both S1 nuclease analysis and primer extension. Although the luciferase cDNA clone lacked the six N-terminal codons ... the missing 5' sequence. The full-length, intronless luciferase gene was inserted into mammalian expression vectors and ... CV-1) cells in which enzymatically active firefly luciferase was transiently expressed. In addition, cell lines stably ...
Tópico(s): Neurobiology and Insect Physiology Research
1987 - Taylor & Francis | Molecular and Cellular Biology
Thomas M. Schmidt, Kamil Kopecký, Kenneth H. Nealson,
... coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited at all stages of growth, although more so during the preinduction phase. Luciferase was purified from cultures of X. luminescens Hm ... and found to be similar to other bacterial luciferases. The Xenorhabdus luciferase consisted of two subunits with approximate molecular masses ... a molecular mass of 24 kilodaltons copurified with luciferase, and in its presence, either NADH or NADPH ...
Tópico(s): Entomopathogenic Microorganisms in Pest Control
1989 - American Society for Microbiology | Applied and Environmental Microbiology
Michael O. Boylan, Jerry Pelletier, Edward A. Meighen,
... the luxA and luxB genes coding for bacterial luciferase has been generated by site-specific mutagenesis so that it is now possible to express luciferase under control of a single promoter in eukaryotes as well as in prokaryotes. The fused luciferase subunits (alpha and beta), linked by a decapeptide, ... and allowed purification of a highly active fused luciferase in the absence of the beta-luciferase subunit. Consequently luciferase activity can be directly attributed to the fused luciferase alone and does not require complementation with free ...
Tópico(s): Molecular Communication and Nanonetworks
1989 - Elsevier BV | Journal of Biological Chemistry
Sung‐Bau Lee, Shi‐Chen Ou, Chung-Fan Lee, Li‐Jung Juan,
... confirmed by DNA sequencing. Plasmids encoding the firefly luciferase gene driven by the HCMV major IE promoter ( ... H. W. Lee. To generate plasmids encoding the luciferase reporter driven by serially 5′-deleted mutants of ... 938-V5/His. pMH100-TK, which contains a luciferase reporter gene driven by the thymidine kinase promoter ... experiments, cells were transfected with the MIEP-driven luciferase reporter and infected with HCMV 4 h post- ... amount (Fig. 2B) for 2 h.Western and Luciferase Assay—Luciferase assays and Western analyses were performed ...
Tópico(s): Cytomegalovirus and herpesvirus research
2009 - Elsevier BV | Journal of Biological Chemistry
Takachika Ito, Shoichi Suzuki, Sachiko Kanaji, Hiroshi Shiraishi, Shoichiro Ohta, Kazuhiko Arima, Go Tanaka, Taro Tamada, Eijiro Honjo, K. Christopher García, Ryota Kuroki, Kenji Izuhara,
... cells transfected with the germ line ϵ promoter-luciferase gene (DND-39/Gϵ cells) were prepared and ... phycoerythrin-labeled anti-CD124 Ab (BD Biosciences). The luciferase assay was performed as previously described (8.Arima ... TTCNNNNGAA), as a promoter sequence of the firefly luciferase gene. The Renilla-luciferase reporter gene (phRL-Tk, Promega, Madison, WI) was ... cell lysates were applied to the Picagene Dual Luciferase assay kit (TOYO Inc., Tokyo, Japan). Iodination of ... lacking the N-terminal 32 amino acids showed luciferase activities similar to the wild type, whereas only ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2009 - Elsevier BV | Journal of Biological Chemistry
Zhican Wang, Gihani T. Wijewickrama, Kuan-wei Peng, Birgit M. Dietz, Long Yuan, Richard B. van Breemen, Judy L. Bolton, Gregory R. J. Thatcher,
... of nanomolar potency in cell culture using a luciferase reporter assay and, using a chromatin immunoprecipitation assay, ... of nanomolar potency in cell culture using a luciferase reporter assay and, using a chromatin immunoprecipitation assay, ... same except omitting gentamicin. Transient Transfection and ERE-Luciferase Assays-Briefly, MCF-7 cells were cultured in ... were transfected with 2 μg of the pERE-luciferase plasmid, which contains three copies of the Xenopus laevis vitellogenin A2 ERE upstream of firefly luciferase. To normalize transfection efficiency, 1 μg of pRL- ...
Tópico(s): Genomics, phytochemicals, and oxidative stress
2009 - Elsevier BV | Journal of Biological Chemistry
Ömer Faruk Hatipoğlu, Satoshi Hirohata, Mehmet Zeynel Cilek, Hiroko Ogawa, Toru Miyoshi, Masanari Obika, Kadir Demircan, Ryoko Shinohata, Shozo Kusachi, Yoshifumi Ninomiya,
... the promoter region of ADAMTS1, and the secreted luciferase assay system demonstrated that hypoxia induced luciferase secretion in the culture medium 4.6-fold ... the promoter region of ADAMTS1, and the secreted luciferase assay system demonstrated that hypoxia induced luciferase secretion in the culture medium 4.6-fold ... for all densitometry analysis. We used the secreted luciferase vector system as previously reported (24Secchiero P. Corallini ... 33) Google Scholar). The structures of ADAMTS1 promoter-luciferase constructs are shown in Fig. 5. To generate ...
Tópico(s): High Altitude and Hypoxia
2009 - Elsevier BV | Journal of Biological Chemistry
Claudia A. Bertuccio, Hannah C. Chapin, Yiqiang Cai, Kavita Mistry, Véronique Chauvet, Stefan Somlo, Michael J. Caplan,
... protein and PC-2 showed an increase in luciferase activity and in CTT expression, indicating that the ... alter intracellular Ca2+ concentrations. PC-2 enhancement of luciferase activity was not altered by any of these ... protein and PC-2 showed an increase in luciferase activity and in CTT expression, indicating that the ... alter intracellular Ca2+ concentrations. PC-2 enhancement of luciferase activity was not altered by any of these ... terminal tail PC-1-Gal4VP16 fusion protein Firefly luciferase Renilla luciferase gadolinium 1,2-bis(o-aminophenoxy) ...
Tópico(s): Genetic Syndromes and Imprinting
2009 - Elsevier BV | Journal of Biological Chemistry
Heng Zhang, Chen‐Ying Liu, Zhengyu Zha, Bin Zhao, Jun Yao, Shimin Zhao, Yue Xiong, Qun‐Ying Lei, Kun‐Liang Guan,
... 24 h post-transfection for protein analysis or luciferase activity assay. To establish stable TAZ-expressing cells, ... solution. The peptides were analyzed by MS/MS. Luciferase Activity Assay—For the luciferase reporter assay, 293T cells were seeded in 24- ... plates. A mixture of 5× upstream activating sequence-luciferase reporter, Renilla, and the indicated plasmids was cotransfected. ... four hours after transfection, cells were lysed, and luciferase activity was measured using a dual-luciferase reporter ...
Tópico(s): Axon Guidance and Neuronal Signaling
2009 - Elsevier BV | Journal of Biological Chemistry
Tsuyoshi Mishiro, Ko Ishihara, Shinjiro Hino, Shuichi Tsutsumi, Hiroyuki Aburatani, Katsuhiko Shirahige, Yoshikazu Kinoshita, Mitsuyoshi Nakao,
... AC3 have enhancer-blocking effects, we performed a luciferase reporter assay in Hep3B cells (Figure 2A). The ... APOC3 enhancer and the APOA4 promoter reduced the luciferase activities to approximately 40–60% of the control ... of the enhancer (pIEALD1, pIEALD2 and pIEALD3). The luciferase activities were not decreased by the AC sites, ... and APOA4 promoter (A4 P) upstream of the luciferase gene. The indicated reporter constructs were introduced into ... cells to examine the enhancer–promoter associations. The luciferase activities from pEALD were normalized to 100. The ...
Tópico(s): RNA modifications and cancer
2009 - Springer Nature | The EMBO Journal
Mei Nie, Saima Aijaz, Isabelle V. Leefa Chong San, María S. Balda, Karl Matter,
... based on two minimal promoters—one driving firefly luciferase expression and the other driving renilla luciferase expression—that differ only by the presence of ... cells were transfected with the ZONAB reporter firefly luciferase construct and the control reporter driving renilla luciferase expression. The used promoter is repressed by ZONAB; ... TAT–C3 fusion protein. After measuring the two luciferase activities, firefly to renilla luciferase ratios were calculated and changes in percentages compared ...
Tópico(s): interferon and immune responses
2009 - Springer Nature | EMBO Reports
Amalraj Thangasamy, Jessica Rogge, Sudhakar Ammanamanchi,
... of –400-bp Wild-type/Mutant RON Promoter-luciferase Reporter Constructs—The 400-bp RON promoter-luciferase reporter was described previously (17Thangasamy A. Rogge J. ... CA) based on the 400-bp RON promoter-luciferase reporter construct as a template. The mutation was ... 5′-GCGTTGCTGTCGTGCGTCCGCAGG-3′; mutant primer, 5′-GCGTTGCTGTAAAGCGTCCGCAGG-3′.Luciferase Assay—Breast cancer cells were seeded into 12- ... Cells were harvested 24 h following transfection, and luciferase activity was measured following normalization to protein levels ( ...
Tópico(s): Axon Guidance and Neuronal Signaling
2009 - Elsevier BV | Journal of Biological Chemistry
Jean‐Baptiste Marq, Stéphane Hausmann, Jeremy Luban, Daniel Kolakofsky, Dominique Garcin,
... pβ-IFN-fl-lucter, which contains the firefly luciferase gene under the control of the human IFN- ... virus TK promoter region upstream of the Renilla luciferase gene (Promega). pEBS-E3L100–190 and pEBS-E3L100– ... were harvested and assayed for firefly and Renilla luciferase activity (dual-luciferase reporter assay system; Promega). Relative expression levels were calculated by dividing the firefly luciferase values by those of Renilla luciferase. Cytoplasmic extracts were prepared using 0.5% Nonidet ... control. Both the level of IFNβ reporter gene (luciferase) activation and the presence of endogenous IFN released ...
Tópico(s): interferon and immune responses
2009 - Elsevier BV | Journal of Biological Chemistry
Ming Du, Kim M. Keeling, Liming Fan, Xiaoli Liu, David M. Bedwell,
... a cystic fibrosis mouse model. Using a dual luciferase readthrough reporter system in cultured cells, we found ... a cystic fibrosis mouse model. Using a dual luciferase readthrough reporter system in cultured cells, we found ... while enhancing its efficacy. Cell Culture and Dual Luciferase Readthrough Assays-HEK293T cells maintained as monolayer cultures ... number P-5387) was from Sigma. For dual luciferase assays, HEK293T cells were split into 96-well ... period. The cells were transfected with the dual luciferase UGA readthrough reporter (pDB691) or CGA sense control ...
Tópico(s): Cystic Fibrosis Research Advances
2009 - Elsevier BV | Journal of Biological Chemistry
Erin A. Kimbrel, Andrew L. Kung,
... Gal4 DNA-binding domain; CyD1-luc, cyclin D1 luciferase; GST, glutathione S-transferase; CMV, cytomegalovirus; TBS, Tris- ... by Ted Dawson (Johns Hopkins University). TOPflash/FOPflash luciferase reporters (pOT-luc and pOF-luc) were from Upstate Biotechnology. pGL4.14-cyclinD1-luciferase, containing 1882 bp of the human cyclin D1 ... from Toshi Shioda (Massachusetts General Hospital). CAGA(12)-luciferase was kindly provided by Baogiang Guo (University of Manchester, UK). p21-luciferase was created by PCR amplification of a 1- ...
Tópico(s): Cancer-related Molecular Pathways
2009 - Elsevier BV | Journal of Biological Chemistry
Tekla Hornakova, Judith Staerk, Yohan Royer, Elisabetta Flex, Marco Tartaglia, Stefan N. Constantinescu, Laurent Knoops, Jean‐Christophe Renauld,
... 20-28Crossref PubMed Scopus (122) Google Scholar). Dual Luciferase Assay-STAT1, STAT3, and STAT5 transcriptional activity was assessed by measurements of luciferase expression in HEK293, COS-7, U4C, and γ2A ... upon transient transfection of appropriate cDNA constructs and luciferase reporter vectors, namely pGL3-pap1-luc, pLHRE-luc, or pGRR5-luc. Another reporter plasmid, Renilla luciferase (pRLTk, Promega), was co-transfected as an internal ... rat β-casein gene promoter, inserted upstream a luciferase gene (27Gerland K. Bataille-Simoneau N. Basle M. ...
Tópico(s): Lymphoma Diagnosis and Treatment
2009 - Elsevier BV | Journal of Biological Chemistry
Yin Gao, Adrienne Howard, Kechen Ban, Joya Chandra,
... capillary genetic analyzer ABI 3100. Construction of Dual-luciferase Expression Plasmid pFynPromoterCheck Vector-Full-length and deletion ... As an internal control, we constructed the Renilla luciferase reporter plasmids containing the firefly luciferase gene in the same plasmid thereby allowing normalization of the Renilla luciferase data. Three DNA fragments: a 4.6-kb ... together to construct the dualluciferase (firefly and Renilla luciferases) expression plasmids. This vector, renamed pFynPromoterCheck, allows the ...
Tópico(s): Acute Myeloid Leukemia Research
2009 - Elsevier BV | Journal of Biological Chemistry
Xiaoliang Liu, Yanyan Zhao, Wang Lu-zeng, Xianghong Yang, Zhihong Zheng, Yuanyuan Zhang, Fangjie Chen, Hong Liu,
... promoter. This heterologous promoter regulated the expression of luciferase and his-tagged CYP4F2 in transfected HEK 293 ... promoter. This heterologous promoter regulated the expression of luciferase and his-tagged CYP4F2 in transfected HEK 293 ... hormonal regulation of kidney androgen-regulated protein (Kap)-luciferase transgenic mice.Toxicol Sci. 2004; 79: 266-277Crossref ... pKAP-CYP4F2/his expression vectors. The expression of luciferase and his-tagged CYP4F2 was detected after transient ... HEK293 cells. As illustrated in Figure 1a, the luciferase activity of cells transfected with the pKAP-LUC, ...
Tópico(s): Alcohol Consumption and Health Effects
2009 - Elsevier BV | Kidney International
Shokoufeh Mahmoodzadeh, Stephan Fritschka, Elke Dworatzek, Thi Hang Pham, Eva Becher, Arne Kuehne, Mercy M. Davidson, Vera Regitz‐Zagrosek,
... by restriction site digestion and sequence analysis. Thereafter, luciferase reporter constructs were generated by using restriction sites ... into promoterless pGl2-basic vector (Promega). The resulting luciferase reporter constructs are referred to as: A-promoter- ... well plates. After 24 h of incubation, promoter-luciferase reporter construct (1 μg) and the internal reference Renilla luciferase reporter plasmid phRL-TK vector (10 ng, Promega) ... cell extracts were prepared, and Firefly and Renilla luciferase activities were sequentially measured using the Dual-GloTM- ...
Tópico(s): Inflammatory mediators and NSAID effects
2009 - Elsevier BV | Journal of Biological Chemistry
L. Policastro, Irene L. Ibañez, Hebe Durán, Gastón Soria, Vanesa Gottifredi, Osvaldo L. Podhajcer,
... the minimal cytomegalovirus (CMV) plasmid upstream of the luciferase reporter gene (Figure 1a).To evaluate the responsiveness ... were placed at different positions relative to the luciferase gene (Figure 1c). The 5′-E6(6)VE- ... as shown by the complete reversal of inducible luciferase activity in the presence of catalase (Figure 1d). The basal luciferase activity driven by E6(40)VE was as ... cells lines with E6(40)VE-LUC, where luciferase activity was driven by the chimeric promoter. As expected, ROS dependent-luciferase activity was higher in malignant cells than in ...
Tópico(s): ATP Synthase and ATPases Research
2009 - Elsevier BV | Molecular Therapy
Ramasamy Paulmurugan, Parasuraman Padmanabhan, Byeong‐Cheol Ahn, Sunetra Ray, Jürgen K. Willmann, Tarik F. Massoud, Sandip Biswal, Sanjiv S. Gambhir,
... Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast ... Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast ... protein p53 along with the reporter gene firefly luciferase in cells and tumor xenografts in living animals. ... derived from adenovirus, and the reporter gene firefly luciferase (FLUC) [(GAL4DNA)5-E4TATA-FLUC], in different co- ... system (Figure 1c). The constitutive transactivation system showed luciferase signal both in the presence and the absence ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2009 - Elsevier BV | Molecular Therapy