Norma Grant, Phil Yates, Anthony Harris, John Naughton, Jon Ashworth, Brendan Martin, Stephen Brook, Mark Mann, Andrew Pierce, Political Correspondent, Andrew Pierce, Adrian Lee, Nigel Colborn, Jack Arkinstall, Jane MacQuitty, Magnus Linklater, Inigo Gilmore, John Diamond, Ivo Tennant, Tim Oliver, Philip Howard, Jeremy Kingston, Joanna Bale, James Bone, Roger Maynard, Kenneth Baker, Kevin Eason, John Jolliffe, Gerald Davies, Clare Stewart, Joe Joseph, Robert Miller, Roger Berthoud, Martin Fletcher, Ruth Gledhill, Religion Correspondent, Robert Sheehan, Roger Bray, John Hopkins, Golf Correspondent, Benet Steinberg, Peter Ball, Ruth Gledhill, Carlton Reid and Kathryn Leigh, Karl Lamers, Peter Davis Director, Libby Purves, Candida Crewe, Ian Morton, Robin Young, Jill Sherman Chief Political Correspondent, Patrick Middleton, Jenny MacArthur, Michael Clark, Jeremy Laurance, Health Correspondent, H. J. Baker, Kea, Kevin Eason Motoring Editor, Richard Stott, Quentin Letts, Philip Argent, Ian Bradley, David Hands, Frances Bissell, Sarah Jones, Sarah Bagnall, Peter Barnard, Michael S. Stainton, David Charter, Philip Bassett, Industrial Editor, Nicholas Wapshott, Derwent May, Kate Muir, Carl Mortished Edited, Lizanne Rose, Russell Kempson, Roger Bell, Stephen Anderton, Sarah Johnson, Barry Millington, Joanne Bower Honorary Secretary, George Plumptre, Gillian Bowditch, Scotland Correspondent, Raymond Keene, Vaughan Freeman, David Charter, Education Correspondent, David Robinson, Alan Lee, Cricket Correspondent, Leyla Linton, Andrew Roberts, Karen Zagor, Frances Gibb, Legal Correspondent, P. H. S, Alix Ramsay, James Allcock, Ian Stirling, Harriet Paterson, Richard Evans, Herb Greer, Eric Reguly, Richard Owen, David Powell, Athletics Correspondent, Oliver Holt, Anne Watson, Erica Wagner, Roger Boyes, Nigel Hawkes, Science Editor, Marcel Berlins, Elliott (Chairman), Kevin Eason and Alan Copps, David Leigh, Julian Muscat, Adam Fresco, Richard Duce, Anne Ashworth, Joanna Pitman, Jack Crossley, Philip Webster Political Editor, Sara McConnell, Rob Hughes, Football Correspondent, Alan Copps, Jonathan Palmer, Karen Kay, Francis Fitzgibbon, Tim Wapshott, Russell Jenkins, John Shaw, Jane Owen, Peter Riddell, Ben MacIntyre, Barry Ward, Helen Pridham, Marlene Dietrich, Tony Dawe, Richard Beeston, Simon Jenkins, Hattie Ellis, Tim Jones, Kate Bassett, Martin Waller, Christopher Irvine, Kyle Smith, Graham Allen, Marianne Curphey and Robert Miller, Jonathan Meades, Marianne Curphey, Jill Crawshaw, Zoe Keniston, Richard Morrison, David Powell, Denis Stevens, Michael Henderson, Fiona Beckett, Christina Rees, Alasdair Murray, Anthony Howard, David Long, David Hands, Rugby Correspondent, Ian Murray and James Landale, Gill Hornby, Jonathan Mirsky, Stacy Sullivan, Dominic Kennedy, Social Services Correspondent, Michael Hornsby Agriculture Correspondent,
... DT Range Rover Vogue 3.9 Registration Numbers Mdk 8 50 RR PJR 6 7 DFC Premier ...
1996 - Gale Group | TDA
Harutoshi Sakakima, Yoshihiro Yoshida, Yoshiki Yamazaki, Fumiyo Matsuda, Masako Ikutomo, Kosei Ijiri, Hisako Muramatsu, Takashi Muramatsu, Kenji Kadomatsu,
... sciatic nerves was explored by comparing wild‐type (Mdk +/+ ) mice with MK‐deficient (Mdk −/− ) mice after freezing injury. In the Mdk −/− mice, a regenerative delay was observed, preceded by ... recovery was delayed compared with that in the Mdk +/+ mice. In the regenerating nerve, unmyelinated axons were ... containing synaptic vesicles disappeared in both mice. In Mdk −/− mice, the appearance of nerve terminals was delayed ... The recovery of evoked electromyogram was delayed in Mdk −/− mice compared with Mdk +/+ mice. Our results suggested ...
Tópico(s): Proteoglycans and glycosaminoglycans research
2009 - Wiley | Journal of Neuroscience Research
Claudia Neunaber, Philip Catalá-Lehnen, Frank Timo Beil, Robert Percy Marshall, Vincent Kanbach, Anke Baranowsky, Wolfgang Lehmann, Thomas Streichert, Anita Ignatius, Takashi Muramatsu, Thorsten Schinke, Michael Amling,
Midkine (Mdk) and pleiotrophin (Ptn) comprise a family of heparin-binding growth factors known primarily for their effects on ... development and remodeling. Together with the finding that Mdk expression increases in the course of primary osteoblast differentiation, we reasoned that Mdk, rather than Ptn, could play a physiologic role in bone formation. Here, we show that Mdk-deficient mice display an increased trabecular bone volume ... differentially affected in trabecular and cortical bone of Mdk-deficient mice. To understand the effect of Mdk ...
Tópico(s): Fibroblast Growth Factor Research
2010 - Oxford University Press | Journal of Bone and Mineral Research
Astrid Liedert, Laura Mattausch, Viktoria Röntgen, Robert Blakytny, Daniel Vogele, Marcus Pahl, Ronny Bindl, Claudia Neunaber, Thorsten Schinke, Sheila Harroch, Michael Amling, Anita Ignatius,
... shown that the deficiency of growth factor midkine (Mdk) in mice at 12 and 18 months of ... may, at least in part, be dependent on Mdk expression. To investigate this, we loaded the ulnae of Mdk-deficient mice and appropriate wild-type mice at ... rate, and bone formation rate in ulnae of Mdk-deficient mice compared to wild-type mice in response to loading. Because Mdk has been shown to bind to a complex ... vitro studies using osteoblastic cells, transiently over-expressing Mdk, Wnt-3a, and Ptprz to evaluate whether Mdk ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2010 - Elsevier BV | Bone
Tomoki Kosugi, Yukio Yuzawa, Waichi Sato, Hanayo Kawai, Seiichi Matsuo, Yoshifumi Takei, Takashi Muramatsu, Kenji Kadomatsu,
... that mice deficient in the growth factor midkine (Mdk−/−) exhibited strikingly milder nephropathy than Mdk+/+ mice, even though both mice showed similar extents ... expression was induced in the glomerular mesangium of Mdk+/+ mice with diabetic nephropathy and in primary cultured mesangial cells exposed to high glucose. Mdk−/− mesangial cells exhibited reduced phosphorylation of protein kinase ... midkine restored extracellular signal-regulated kinase phosphorylation in Mdk−/− cells under high glucose conditions, whereas a midkine ...
Tópico(s): Diabetes and associated disorders
2006 - Elsevier BV | American Journal Of Pathology
Hio Chung Kang, Il-Jin Kim, Hyewon Park, Sang‐Geun Jang, Sun-A Ahn, Sang Nam Yoon, Hee-Jin Chang, Byong Chul Yoo, Jae-Gahb Park,
MDK is a heparin-binding growth factor associated with cancer development. Here, we sought to examine the association of MDK expression with resistance and sensitivity to different chemotherapeutic agents. We established stable HeLa cell transfectants (HeLa–MDK) and tested for decreased sensitivity to chemotherapeutic agents ( ... cisplatin). In addition, we used siRNA to block MDK expression in SNU-638 human gastric cancer cells and examined the chemosensitizing effect. HeLa–MDK cells treated with 5-FU, doxorubicin, and cisplatin ...
Tópico(s): Neuroblastoma Research and Treatments
2006 - Elsevier BV | Cancer Letters
Anna Nordin, Wanzhong Wang, Karin Welén, Jan‐Erik Damber,
... CRPC, is needed. Expression of growth factor midkine (MDK) is correlated with poor clinical outcomes in various ... PC. In the present study, we have evaluated MDK expression and NED in two separate tumor groups: early and advanced PC.Immunohistochemical analysis of MDK, the neuronal marker tubulin-beta III (TUBB3) and ... imaging was performed on a selection of specimens.MDK, TUBB3, and CGA were upregulated in CRPC compared to HN tumors. MDK was highly associated to the expression of both CGA and TUBB3, and identified MDK-positive NE-like looking cells found to co- ...
Tópico(s): Fibroblast Growth Factor Research
2012 - Wiley | The Prostate
Yingying Xu, Xiaoyun Mao, Yongxi Song, Feng Zhao, Zhenning Wang, Wei-xu Zhang, Huimian Xu, Feng Jin,
Midkine (MDK) is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis, which is ... current study was to investigate the mechanism of MDK involved in the Adriamycin (ADR) resistance in human ... ADR compared with Adriamycin-sensitive SGC7901 cell line. MDK mRNA and protein expression levels were significantly higher ... gain a deeper insight into the role of MDK in SGC7901/ADR, we stably transfected Adriamycin-sensitive SGC7901 with viral vector expressing MDK. Our result showed that multidrug resistance type I ( ...
Tópico(s): Fibroblast Growth Factor Research
2012 - SAGE Publishing | Tumor Biology
Jingyan Luo, Xiaoxiao Wang, Zhibo Xia, Lixuan Yang, Zhi‐Ming Ding, Shiyuan Chen, Bingquan Lai, Nu Zhang,
Midkine (MDK) expression is associated with the proliferation of many cancers, including glioma. However, the upstream signaling that leads to MDK accumulation remains elusive. This study investigates the molecular mechanism that induces MDK overexpression in human glioma. The Repository for Molecular Brain Neoplasia Data was analyzed to identify potential MDK regulators. Expression of MDK and specificity protein 1 (SP1) was compared in ... assay was used to confirm the transcriptional regulation. MDK-force-expressed, SP1-silenced glioma cells were used ...
Tópico(s): Ubiquitin and proteasome pathways
2014 - American Society for Cell Biology | Molecular Biology of the Cell
Melanie Haffner‐Luntzer, Aline Heilmann, Anna E. Rapp, Simon Thomas Beie, Thorsten Schinke, Michael Amling, Anita Ignatius, Astrid Liedert,
The growth and differentiation factor midkine (Mdk) plays an important role in bone development and remodeling. Mdk-deficient mice display a high bone mass phenotype when aged 12 and 18 months. Furthermore, Mdk has been identified as a negative regulator of ... pro-inflammatory effects. Together with the finding that Mdk is expressed in chondrocytes during fracture healing, we hypothesized that Mdk could play a complex role in endochondral ossification ... an external fixator were created in wildtype and Mdk-deficient mice. Fracture healing was evaluated 4, 10, ...
Tópico(s): Fibroblast Growth Factor Research
2014 - Public Library of Science | PLoS ONE
Huifang Hao, Yutaka Maeda, Takuya Fukazawa, Tomoki Yamatsuji, Munenori Takaoka, Xiao-Hong Bao, Junji Matsuoka, Tatsuo Okui, Tsuyoshi Shimo, Nagio Takigawa, Yasuko Tomono, Motowo Nakajima, Iris M. Fink-Baldauf, Sandra L. Nelson, William Seibel, Ruben Papoian, Jeffrey A. Whitsett, Yoshio Naomoto,
Midkine (MDK) is a heparin-binding growth factor that is highly expressed in many malignant tumors, including lung cancers. MDK activates the PI3K pathway and induces anti-apoptotic ... the survival of tumors. Therefore, the inhibition of MDK is considered a potential strategy for cancer therapy. ... a novel small molecule compound (iMDK) that targets MDK. iMDK inhibited the cell growth of MDK-positive H441 lung adenocarcinoma cells that harbor an ... iMDK did not reduce the cell viability of MDK-negative A549 lung adenocarcinoma cells or normal human ...
Tópico(s): Fibroblast Growth Factor Research
2013 - Public Library of Science | PLoS ONE
Donghong He, Hu Chen, Hisako Muramatsu, Amy W. Lasek,
... Alcohol engages signaling pathways in the brain. Midkine ( MDK ) is a neurotrophic factor that is over‐expressed in the prefrontal cortex of alcoholics. MDK and one of its receptors, anaplastic lymphoma kinase ( ... goal of this study was to determine whether MDK and ALK expression and signaling are activated by ... found that ethanol treatment of neuroblastoma cells increased MDK and ALK expression. We also assessed activation of ... ethanol exposure. Similarly, treatment of cells with recombinant MDK protein increased ALK , ERK and STAT3 phosphorylation, suggesting ...
Tópico(s): Fibroblast Growth Factor Research
2015 - Wiley | Journal of Neurochemistry
Karim Shaheen, A I Abdelmageed, Eslam Safwat, Ashraf M. Albreedy,
... is needed. We aimed to evaluate serum midkine (MDK) as a biomarker for HCC diagnosis. Patients and ... patients, and 30 healthy subjects were enrolled. Serum MDK using ELISA was measured in all included subjects. Results. Serum MDK was significantly elevated in HCC group compared to ... mL), respectively. No significant association was found between MDK and either BCLC stage, tumor diameter, tumor number, ... operating characteristic curve showed that best cutoff for MDK and AFP was 0.387 and 88.5 ng/mL, respectively. Area under the curve of MDK was significantly larger than that of AFP (0. ...
Tópico(s): Fibroblast Growth Factor Research
2015 - Hindawi Publishing Corporation | International Journal of Hepatology
Melanie Haffner‐Luntzer, Julia Kemmler, Verena Heidler, Katja Prystaz, Thorsten Schinke, Michael Amling, Anna Kovtun, Anna E. Rapp, Anita Ignatius, Astrid Liedert,
The heparin-binding growth and differentiation factor midkine (Mdk) is proposed to negatively regulate osteoblast activity and bone formation in the adult skeleton. As Mdk-deficient mice were protected from ovariectomy (OVX)-induced ... of postmenopausal osteoporosis. We have previously demonstrated that Mdk negatively influences bone regeneration during fracture healing. Here, we investigated whether the inhibition of Mdk using an Mdk-antibody (Mdk-Ab) improves compromised bone healing in osteoporotic OVX- ...
Tópico(s): Protease and Inhibitor Mechanisms
2016 - Public Library of Science | PLoS ONE
Roslyn Vongsuvanh, David van der Poorten, Tristan J. Iseli, Simone I. Strasser, Geoffrey W. McCaughan, Jacob George,
... HCC) surveillance are lacking. We compared serum midkine (MDK), dickkopf-1 (DKK1), osteopontin (OPN) and AFP for ... at and before cancer diagnosis. Serum levels of MDK and OPN were higher in HCC patients compared ... cohort, 59.18% (n = 29/49) had elevated MDK, applying the optimal cut-off of 0.44 ng/ml. Using AFP ≥ 20 IU/ml or MDK ≥ 0.44 ng/ml, a significantly greater number ( ... The area under the receiver operating curve for MDK was superior to AFP and OPN in NASH-HCC diagnosis. In the longitudinal cohort, MDK was elevated in 15/28 (54%) of HCC ...
Tópico(s): Liver Disease Diagnosis and Treatment
2016 - Public Library of Science | PLoS ONE
Hu Chen, Dongjing He, Amy W. Lasek,
Midkine (MDK) is a cytokine and neurotrophic factor that is more highly expressed in the brains of alcoholics and ... to drink large amounts of ethanol, suggesting that MDK may regulate ethanol consumption. Here we measured ethanol consumption in male and female Mdk knockout (-/-) mice using the two-bottle choice and ... in the dark (DID) tests. We found that Mdk -/- mice consumed significantly more ethanol than wild-type controls in both tests. To determine if MDK acts in the ventral tegmental area (VTA) to regulate ethanol consumption, we delivered lentivirus expressing a Mdk shRNA into the VTA of male C57BL/6J ...
Tópico(s): Chemokine receptors and signaling
2017 - Wiley | Genes Brain & Behavior
Chih‐Kai Hong, Cheng‐Li Lin, Fa‐Chuan Kuan, Ping-Hui Wang, Ming‐Long Yeh, Wei‐Ren Su,
... were evaluated, namely, the McKeon's double Krackow (MDK) suture, Wilson's double Krackow (WDK) suture, and ... differences in elongation after cyclic loading among the MDK suture (7.9 ± 3.6 mm, 14% ± 6%), ... showed that elongation after cyclic loading in the MDK suture was significantly less than the WDK suture ( ... In this porcine in vitro biomechanical study, the MDK suture had significantly smaller elongation after cyclic loading ... ultimate failure load was not different across the MDK, WDK, and OMK sutures. Clinical Relevance Smaller elongation ...
Tópico(s): Tendon Structure and Treatment
2017 - Elsevier BV | Arthroscopy The Journal of Arthroscopic and Related Surgery
Carolina Cubillos‐Zapata, Miguel Ángel Martínez‐García, Elena Díaz‐García, Víctor Toledano, Francisco Campos‐Rodríguez, Manuel Sánchez‐de‐la‐Torre, Eduardo Nagore, A. Martorell, Luis Blasco, Esther Pastor, Jorge Abad, Josep M. Montserrat, Valentín Cabriada, Irene Cano‐Pumarega, Jaime Corral‐Peñafiel, Eva Arias, Olga Mediano, María Somoza‐González, Joan Dalmau‐Arias, Isaac Almendros, Ramón Farré, Eduardo López‐Collazo, David Gozal, Francisco García‐Río,
Midkine (MDK) might mediate the proangiogenic effect of intermittent hypoxia (IH) in patients with obstructive sleep apnea (OSA) and cutaneous melanoma (CM). We compare circulating MDK in CM patients with and without OSA, and ... aggressiveness, while exploring in vitro effects of soluble MDK on human lymphatic endothelial (HLEC) and melanoma cell proliferation. In 360 CM patients, sleep studies and MDK serum level measurements were performed. The effect of MDK on cell proliferation was assessed using HLEC and ...
Tópico(s): Salivary Gland Tumors Diagnosis and Treatment
2020 - Wiley | The FASEB Journal
Seiya Takada, Harutoshi Sakakima, Takahiro Matsuyama, Shotaro Otsuka, Kazuki Nakanishi, Kosuke Norimatsu, Yuki Itashiki, Akira Tani, Kiyoshi Kikuchi,
... and neuronal apoptosis activity, after TBI.Wild type (Mdk+/+) and MK gene deficient (Mdk-/-) mice were subjected to fluid percussion injury for ... phenotype markers, and apoptotic activity.As opposed to Mdk+/+ mice, Mdk-/- mice reported a significantly reduced area of brain ... in the perilesional site were significantly decreased in Mdk-/- than in the Mdk+/+ mice at 3 days after TBI. However, the ... CD16/32) immunoreactive areas were significantly reduced in Mdk-/- than in the Mdk+/+ mice. Likewise, the mRNA ...
Tópico(s): S100 Proteins and Annexins
2020 - BioMed Central | Journal of Neuroinflammation
Dong Hoon Shin, Jeong Yeon Jo, Sun Ha Kim, Min-Young Choi, Chungyong Han, Beom K. Choi, Sang Soo Kim,
... the HIF-1α expression pattern. Among them, midkine (MDK) expression was affected the most in response to HIF-1α. MDK is a heparin-binding growth factor that promotes angiogenesis and carcinogenesis. Indeed, MDK significantly increased HUVEV endothelial cell migration and neo- ... assay (CAM) assay via paracrine signaling. In addition, MDK secreted from NSCLC cells interacted with Notch2 which ... and increased cancer promotion. However, in response to MDK knock down, siRNA or the MDK inhibitor, iMDK treatment not only decreased MDK-induced ...
Tópico(s): Proteoglycans and glycosaminoglycans research
2020 - Multidisciplinary Digital Publishing Institute | Cancers
Beiquan Hu, Chao Qin, Li Li, Lei Wei, Xianlun Mo, Huasheng Fan, Yuanhua Lei, Feng Wei, Donghua Zou,
... Our previous bioinformatics-based study found that midkine (MDK) was associated with poor prognosis of glioblastoma (GBM). However, the mechanism of MDK in GBM remains elusive. Methods A public GBM- ... center were used validate the aberrant expression of MDK in GBM at the RNA and protein levels. The relationship between MDK expression and survival of GBM patients was also explored through survival analysis. Subsequently, we identified MDK-related GBM-specific genes using differential expression analysis. ...
Tópico(s): Cancer-related molecular mechanisms research
2021 - BioMed Central | Cancer Cell International
Arezoo Gowhari Shabgah, Fatemeh Ezzatifar, A. Surendar, Angelina Olegovna Zekiy, Majid Ahmadi, Seyed Mohammad Gheibihayat, Jamshid Gholizadeh Navashenaq,
... from highly complex molecular and pathological pathways. Midkine (MDK) is a growth factor that impacts viability, migration, and other cell activities. Since MDK has been involved in the inflammatory responses, it has been claimed that MDK has a crucial role in HCC. MDK acts as an anti-apoptotic factor, which mediates tumor cell viability. In addition, MDK blocks anoikis to promote metastasis. There is also evidence that MDK is involved in angiogenesis. It has been shown that the application of anti-MDK approaches might be promising in the treatment of ...
Tópico(s): Fibroblast Growth Factor Research
2021 - Wiley | IUBMB Life
Warren A. Campbell, Amanda Fritsch‐Kelleher, Isabella Palazzo, Thanh Hoang, Seth Blackshaw, Andy J. Fischer,
Abstract Recent studies suggest midkine (MDK) is involved in the development and regeneration of the zebrafish retina. We investigate the expression patterns of MDK and related factors, roles in neuronal survival, and ... using single‐cell RNA‐sequencing, we find that MDK and pleiotrophin ( PTN ), a MDK‐related cytokine, are upregulated by Müller glia (MG) ... of development in chick. While PTN is downregulated, MDK is dramatically upregulated in mature MG after retinal damage or FGF2 and insulin treatment. By comparison, MDK and PTN are downregulated by MG in damaged ...
Tópico(s): Retinal Diseases and Treatments
2021 - Wiley | Glia
Quan Zhou, Chen Yang, Zezhong Mou, Siqi Wu, Xiyu Dai, Xinan Chen, Yuxi Ou, Limin Zhang, Jianjun Sha, Haowen Jiang,
Recent studies identified Midkine (MDK) as playing a key role in immune regulation. In this study, we aimed to discover the clinical significance and translational ... was applied for evaluating the targeting potential of MDK. We found that intratumoral MDK expression correlated with PCa progression, which indicated an ... free survival for postoperative PCa patients. Addition of MDK expression to the postoperative risk assessment tool CAPRA-S could improve its prognostic value. Tumors with MDK abundance characterized the tumor-infiltrating CD8+ T cells ...
Tópico(s): Proteoglycans and glycosaminoglycans research
2022 - Wiley | Cancer Science
Luyu Zheng, Qun Liu, Ruijun Li, Shibin Chen, Jingyu Tan, Lina Li, Xichen Dong, Changzhi Huang, Tao Wen, Jian Liu,
... cell line HCT116. We further disclosed that midkine (MDK), an emerging oncoprotein and EMT inducer, is a ... in these cell lines. Mechanistically, IFN-γ upregulated MDK via STAT1, a principle downstream effector in the IFN-γ signalling. MDK is elevated in the majority of cancer types ... cancer metastasis in all examined cell lines. Targeting MDK using a specific MDK inhibitor (iMDK) broadly reversed IFN-γ-activated EMT, ... γ-triggered metastasis. Collectively, our data uncover a MDK-dependent EMT inducing mechanism underlying IFN-γ-driven ...
Tópico(s): Glycosylation and Glycoproteins Research
2022 - Frontiers Media | Frontiers in Oncology
... extent of tubulointerstitial injuries in MK wild-type [Mdk(+/+)] and knockout [Mdk(-/-)] mice 90 min after IRI. MK was expressed weakly in the proximal tubules in Mdk(+/+) mouse kidneys. After IRI, MK expression in proximal ... expression was observed in the distal tubules in Mdk(+/+) mice. Immediate induction of MK expression was observed ... inflammatory leukocytes such as neutrophils and macrophages in Mdk(-/-) mice than in Mdk(+/+) mice. Marked up-regulation of monocyte chemoattractant protein ... 1) and MIP-2 expression was detected in Mdk(+/+) mouse kidneys. Tubulointerstitial damage observed after IRI was ...
Tópico(s): Cardiac and Coronary Surgery Techniques
2002 - Oxford University Press | Nephrology Dialysis Transplantation
Arturo R. Maldonado, Chuck Klanke, Anil G. Jegga, Bruce J. Aronow, Yonatan Y. Mahller, Timothy P. Cripe, Timothy M. Crombleholme,
Abstract Background Expression profile analyses of midkine (MDK), a multifunctional protein important in development but repressed postnataly, indicate that it is highly expressed in approximately 80% of adult carcinomas and many ... capable of targeting developmentally primitive cancers that express MDK. Methods We sought to increase the oncolytic efficacy of the virus by fusing the human MDK promoter to the HSV type 1 neurovirulence gene, ... protein product increases viral replication. Results Tissue‐specific MDK promoter activity in human tumor cells and transgene ...
Tópico(s): Herpesvirus Infections and Treatments
2010 - Wiley | The Journal of Gene Medicine
Chien‐Min Chen, Dueng‐Yuan Hueng, Yen-Po Cheng, Chin Lin, Ping-Yi Lin, Chun‐Yuan Cheng, Hsin‐I Ma,
... prognosis and predictive biomarkers is very important. Midkine (MDK), a heparin-binding growth factor, is important in ... This study aimed to identify over-expression of MDK in gliomas and correlate this with clinical outcomes. ... data of 100 and 180 patients, respectively. The MDK expression, World Health Organization (WHO) pathological grade, sex, ... A search of the GEO profile revealed that MDK expression level was statistically greater in the WHO ... By the Kaplan-Meier survival curve, the high MDK expression group had poorer survival outcome (2.38- ...
Tópico(s): Caveolin-1 and cellular processes
2014 - Medknow | Surgical Neurology International
Suat Erdoğan, Zeynep Banu Doğanlar, Oğuzhan Doğanlar, Kader Turkekul, Rıza Serttas,
Midkine (MDK) is a tumor-promoting factor that is often overexpressed in various human carcinomas, and the role of MDK has not yet been fully investigated in prostate ... cells. PCSCs were treated with different concentrations of MDK inhibitor, iMDK, for 24-72 hours. The IC50 values were determined by the MTT test. Endogenous MDK messenger RNA expression was knocked down by small ... by small interfering RNA-mediated knockdown of endogenous MDK expression. iMDK was shown to preferentially induce cell ... either of the agents used alone. Inhibition of MDK expression strongly suppressed the migration of PCSCs compared ...
Tópico(s): Glycosylation and Glycoproteins Research
2017 - Elsevier BV | The American Journal of the Medical Sciences