Miguel Leão, Sara Gomes, José Pedraza‐Chaverrí, Neuza Machado, Emı́lia Sousa, Madalena Pinto, Alberto Inga, Clara Pereira, Lucı́lia Saraiva,
α-Mangostin (1) and gambogic acid (2) are natural products with potent cytotoxic activity against several human tumor cells. However, their molecular mechanisms of action remain controversial. In this work, using yeast-based assays, it was shown that both xanthones are potential inhibitors of the p53–MDM2 interaction. This activity on p53–MDM2 interaction was confirmed by a gene reporter assay in a human tumor cell. Additionally, computational docking studies supported the potential of these xanthones ...
Tópico(s): Carbohydrate Chemistry and Synthesis
2013 - American Chemical Society | Journal of Natural Products
Miguel Leão, Clara Pereira, Alessandra Bisio, Yari Ciribilli, Ana M. Oliveira Paiva, Neuza Machado, Andreia Palmeira, Miguel X. Fernandes, Emı́lia Sousa, Madalena Pinto, Alberto Inga, Lucı́lia Saraiva,
The virtual screening of a library of xanthone derivatives led us to the identification of potential novel MDM2 ligands. The activity of these compounds as inhibitors of p53–MDM2 interaction was investigated using a yeast phenotypic assay, herein developed for the initial screening. Using this approach, in association with a yeast p53 transactivation assay, the pyranoxanthone (3,4-dihydro-12-hydroxy-2,2-dimethyl-2H,6H-pyrano[3,2-b]xanthen-6-one) (1) was identified as a putative small-molecule inhibitor ...
Tópico(s): Ubiquitin and proteasome pathways
2013 - Elsevier BV | Biochemical Pharmacology
Ana PatrÃcia Graça, Flávia Viana, Joana Bondoso, Maria InÃas Correia, Luís Gomes, Madalena Humanes, Alberto Reis, Joana R. Xavier, Helena Gaspar, Olga Maria Lage,
Interest in the study of marine sponges and their associated microbiome has increased both for ecological reasons and for their great biotechnological potential. In this work, heterotrophic bacteria associated with three specimens of the marine sponge Erylus deficiens, were isolated in pure culture, phylogenetically identified and screened for antimicrobial activity. The isolation of bacteria after an enrichment treatment in heterotrophic medium revealed diversity in bacterial composition with only ...
Tópico(s): Antimicrobial Peptides and Activities
2015 - Frontiers Media | Frontiers in Microbiology
Miguel Leão, Joana Soares, Sara Gomes, Liliana Raimundo, Helena Ramos, Cláudia Bessa, Glória Queiróz, Sofia Domingos, Madalena Pinto, Alberto Inga, Honorina Cidade, Lucı́lia Saraiva,
Chalcones are naturally occurring compounds with recognized anticancer activity. It was recently shown that the O-prenyl derivative (2) of 2'-hydroxy-3,4,4',5,6'-pentamethoxychalcone (1) had a remarkably increased cytotoxicity against human tumour cells compared to its precursor. With this study, we aimed to investigate the molecular mechanism underlying the improved tumour cytotoxicity of prenylchalcone 2.The impact of chalcones 1 and 2 on p53-MDM2 interaction was investigated using yeast growth- ...
Tópico(s): Synthesis and biological activity
2015 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Fernando Madalena Volpe, José Alberto Del-Porto,
Seasonality of mania has been previously reported in several world regions. A spring and/or summer peak has been the most frequent finding, correlating to climatic variables, especially luminosity. There are, however, no South American studies on this association.The charts of 269 manic patients admitted from 1996 to 2000 in a psychiatric hospital at Belo Horizonte, Brazil, were reviewed. Seasonality was assessed with Cosinor Analysis. Correlations of the rate of admissions for mania to climatic ...
Tópico(s): Mental Health Research Topics
2006 - Elsevier BV | Journal of Affective Disorders
Lucrezia Martino, Madalena V. Cruz, Alberto Scoma, Filomena Freitas, Lorenzo Bertin, Mariastella Scandola, Maria A.M. Reis,
Used cooking oil (UCO) was employed as the sole carbon source for the production of polyhydroxybutyrate (PHB) by cultivation in batch mode of Cupriavidus necator DSM 428. The produced biomass was used for extraction of the PHB granules with a solvent-free approach using sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and the enzyme Alcalase in an aqueous medium. The recovered PHB granules showed a degree of purity higher than 90% and no crystallization (i.e., granules were recovered ...
Tópico(s): Biofuel production and bioconversion
2014 - Elsevier BV | International Journal of Biological Macromolecules
Produção Nacional
Revisado por pares
Cristiane Duque, Thaís de Cássia Negrini, Nancy Tomoko Sacono, Denise Madalena Palomari Spolidório, Carlos Alberto de Souza Costa, Josimeri Hebling,
Tópico(s): Dental materials and restorations
2009 - Springer Nature | Clinical Oral Investigations
Produção Nacional
Revisado por pares
A.L. Miranda, H. Roche, Marco Antônio Ferreira Randi, Madalena Lima Menezes, Ciro Alberto de Oliveira Ribeiro,
For assessing the impact of chlorinated compounds, such as organochlorine pesticides, polychlorinated biphenyls, chlorotriazines (atrazine, simazine), and chlorinated phenylureas (diuron), on the Ponta Grossa lake South of Brazil, ten freshwater trahira fish (Hoplias malabaricus) were collected in October 2005. The contamination status was evaluated by the energy budget and various histopathological markers. The results showed detectable amounts of persistent organic pollutants (POPs) in the liver and ...
Tópico(s): Microplastics and Plastic Pollution
2008 - Elsevier BV | Environment International
Hérica Adad Ricci, Débora Lopes Salles Scheffel, Matheus Racy Mariusso, Denise Madalena Palomari Spolidório, Carlos Alberto de Souza Costa, Josimeri Hebling,
To evaluate the effect of saturation of demineralized dentin with aqueous and alcoholic excipients of chlorhexidine (CHX) on the exposure of collagen fibrils in resin-dentin bonds in sound and caries-affected dentin.Flat midcoronal dentin surfaces were prepared from 24 noncarious molars, and artificial caries was induced in half of the sample. For each substrate, the surfaces were assigned to 4 groups (n = 3) according to the saturation solution of the dentin: water, ethanol, 1% CHX aqueous or alcoholic ...
Tópico(s): Bone and Dental Protein Studies
2014 - Quintessence Publishing Company | Journal of adhesive dentistry/The journal of adhesive dentistry
Fernando Ribeiro, Alberto J. Alves, Madalena Teixeira, Fátima Miranda, Cristina Azevedo, José Alberto Duarte, José Oliveira,
Heart rate recovery, defined as the fall in heart rate during the first minute after exercise, is an indicator of autonomic function, and has been found to be an independent predictor of mortality after acute myocardial infarction. Exercise training has several well-known benefits in terms of cardiorespiratory fitness, modifiable cardiovascular risk factors and prognosis after acute coronary events. However, there are no randomized controlled studies in the literature evaluating the effects of exercise ...
Tópico(s): Cardiac Health and Mental Health
2012 - Elsevier BV | Revista Portuguesa de Cardiologia
Madalena Vieira‐Pinto, João R. Alberto, José Aranha, João Serejo, Ana Canto, Mónica V. Cunha, Ana Botelho,
Tópico(s): Infectious Diseases and Tuberculosis
2011 - Springer Science+Business Media | European Journal of Wildlife Research
Nórton Luís Oliveira, Fernando Ribeiro, Madalena Teixeira, Lilibeth Campos, Alberto J. Alves, Gustavo Silva, José Oliveira,
The purpose of this study is to evaluate the effects of an 8-week exercise-based cardiac rehabilitation program on traditional and nonlinear heart rate variability (HRV) indexes, assessing the potential confounding influences of habitual physical activity (PA) and dietary intake. In this parallel-group trial, 96 patients (56 ± 10 years old) were randomized to the exercise group (EG) or to the control group (CG) 4 weeks after an acute myocardial infarction. Exercise-based cardiac rehabilitation program ...
Tópico(s): Cardiovascular and exercise physiology
2014 - Elsevier BV | American Heart Journal
Diana Martins, Bárbara Sousa, Nair Lopes, Madalena Gomes, Luiz Alberto Veronese, André Albergaria, Joana Paredes, Fernando Schmitt,
The current system of pathologic classification of human breast cancers does not take into account the biologic determinants of prognosis, nor is there a consensus regarding the progression from in situ to invasive carcinoma. The present study compared the molecular phenotypes of in situ and invasive components of breast cancer in the same sample. We built a series of 189 in situ and invasive carcinomas using tissue microarrays and classified them according to their immunoprofiles regarding estrogen ...
Tópico(s): Cancer Cells and Metastasis
2011 - Elsevier BV | Human Pathology
Produção Nacional
Revisado por pares
Fernando Madalena Volpe, Almir Tavares, José Alberto Del Porto,
To investigate the existence of a seasonal distribution of three dimensions of mania, psychosis, aggression and suicidality, during psychiatric hospitalizations, in a Brazilian sample, correlating these findings with local climatic variables. Charts of 425 admissions of 269 ICD-10 manic patients, from 1996–2000 were reviewed for the occurrence of these symptom dimensions. Seasonality was assessed using Cosinor Analysis. When a harmonic seasonal distribution was not present, chi-square tests comparing ...
Tópico(s): Suicide and Self-Harm Studies
2007 - Elsevier BV | Journal of Affective Disorders
Niels Kruse, Staffan Persson, Daniel Alcolea, Justyna M.C. Bahl, Inês Baldeiras, Elisabetta Capello, Davide Chiasserini, Luisella Bocchio‐Chiavetto, Andreja Emeršič, Sebastiaan Engelborghs, Erden Eren, Tormod Fladby, Giovanni B. Frisoni, María‐Salud García‐Ayllón, Şermin Genç, Olymbia Gkatzima, Niels H. H. Heegaard, André M. Janeiro, Branislav Kováčech, H. Bea Kuiperij, Maria João Leitão, Alberto Lleó, Madalena Martins, Mafalda Matos, Hanne M. Møllergård, Flavio Nobili, Annika Öhrfelt, Lucilla Parnetti, Catarina R. Oliveira, Uroš Rot, Javier Sáez‐Valero, Hanne Struyfs, Julia T. Tanassi, Peggy Taylor, Magda Tsolaki, Eugeen Vanmechelen, Marcel M. Verbeek, Norbert Žilka, Kaj Blennow, Henrik Zetterberg, Brit Mollenhauer,
Decreased levels of alpha-synuclein (aSyn) in cerebrospinal fluid (CSF) in Parkinson's disease and related synucleinopathies have been reported, however, not consistently in all cross-sectional studies. To test the performance of one recently released human-specific enzyme-linked immunosorbent assay (ELISA) for the quantification of aSyn in CSF, we carried out a round robin trial with 18 participating laboratories trained in CSF ELISA analyses within the BIOMARKAPD project in the EU Joint Program - Neurodegenerative ...
Tópico(s): Neurological disorders and treatments
2015 - Elsevier BV | Neurobiology of Aging
Produção Nacional
Revisado por pares
Fernanda Gonçalves Basso, Camila F. Oliveira, Amanda Fontana, Cristina Kurachi, Vanderlei Salvador Bagnato, Denise Madalena Palomari Spolidório, Josimeri Hebling, Carlos Alberto de Souza Costa,
The aim of this study was to evaluate the effect of specific parameters of low-level laser therapy (LLLT) on biofilms formed by Streptococcus mutans, Candida albicans or an association of both species. Single and dual-species biofilms - SSB and DSB - were exposed to laser doses of 5, 10 or 20 J/cm2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm, 0.04 W). After irradiation, the analysis of biobilm viability (MTT assay), biofilm growth (cfu/mL) and cell morphology (SEM) showed that ...
Tópico(s): Dental materials and restorations
2011 - Fundação Odontológica de Ribeirão Preto | Brazilian Dental Journal
Produção Nacional
Revisado por pares
Nair Lopes, Bárbara Sousa, Diana Martins, Madalena Gomes, Daniella Serafim Couto Vieira, Luiz Alberto Veronese, Fernanda Milanezi, Joana Paredes, José Luís Costa, Fernando Schmitt,
Breast cancer is a heterogeneous disease associated with different patient prognosis and responses to therapy. Vitamin D has been emerging as a potential treatment for cancer, as it has been demonstrated that it modulates proliferation, apoptosis, invasion and metastasis, among others. It acts mostly through the Vitamin D receptor (VDR) and the synthesis and degradation of this hormone are regulated by the enzymes CYP27B1 and CYP24A1, respectively. We aimed to study the expression of these three ...
Tópico(s): Cancer Risks and Factors
2010 - BioMed Central | BMC Cancer
Nórton Luís Oliveira, Fernando Ribeiro, Alberto J. Alves, Madalena Teixeira, Fátima Miranda, José Oliveira,
Heart rate variability (HRV) is a simple and noninvasive measure that estimates cardiac autonomic modulation, mainly the parasympathetic contribution. Increased sympathetic and/or decreased parasympathetic nervous activity is seen in post-myocardial infarction (MI) patients. Consequently, these patients present reduced HRV, which has been associated with increased risk of adverse events and mortality. Exercise training, recommended as a complementary therapy for patients with cardiovascular disease, ...
Tópico(s): Non-Invasive Vital Sign Monitoring
2013 - Elsevier BV | Revista Portuguesa de Cardiologia
Mónica V. Cunha, Filipa Matos, Ana Canto, Teresa Albuquerque, João R. Alberto, José Aranha, Madalena Vieira‐Pinto, Ana Botelho,
Tópico(s): Infectious Diseases and Tuberculosis
2011 - Elsevier BV | Research in Veterinary Science
Gil Machado, E. Franců, Milada Vavrdová, Deolinda Flores, Paulo E. Fonseca, Fernando Rocha, Luís C. Gama Pereira, António Alberto Gomes, Madalena Fonseca, Helder I. Chaminé,
The Albergaria-a-Velha Unit is one of several tectonostratigraphic out-of-sequence units of the metamorphic belt associated with the Porto–Tomar shear zone (Ossa–Morena Zone, W Portugal). It is composed of considerably deformed – very low grade – metasediments, namely shales, siltstones and rare fine sandstones. In this work we present new sedimentological and biostratigraphical data that suggest the Albergaria-a-Velha Unit was deposited from the (?)early Frasnian to the Serpukhovian in a distal marine ...
Tópico(s): Paleontology and Stratigraphy of Fossils
2011 - Polish Geological Institute | Geological Quarterly
Cristina Jácome, Ana Margarida Pereira, Rute Almeida, Manuel Ferreira‐Magalhães, Mariana Couto, Luís Araújo, Mariana Pereira, Magna Alves‐Correia, Cláudia Chaves Loureiro, Maria Joana Catarata, Lília Maia Santos, João Gonçalves-Pereira, Bárbara Ramos, Cristina Lopes, Ana Mendes, José Carlos Cidrais Rodrigues, Georgeta Oliveira, Ana Paula Aguiar, Ivete Afonso, Joana Carvalho, Ana Arrobas, José Coutinho Costa, Joana Alves Dias, Ana Todo‐Bom, João Azevedo, Carmelita Ribeiro, Marta Alves, Paula Pinto, Nuno Neuparth, Ana Palhinha, João Gaspar Marques, Nicole Pinto, Pedro Martins, Filipa Todo Bom, Maria Alvarenga Santos, Alberto Costa, Armandina Silva Neto, Marta Santalha, Carlos Lozoya, Natacha Santos, Diana Silva, Maria João Vasconcelos, Luís Taborda‐Barata, Célia Carvalhal, Maria Fernanda Teixeira, Rodrigo Rodrigues Alves, Ana Sofia Moreira, Cláudia Sofia Pinto, Pedro Morais Silva, Carlos Alves, Raquel Câmara, Didina Coelho, Diana Bordalo, Ricardo M. Fernandes, Rosário Ferreira, Fernando Menezes, Ricardo Gomes, Maria José Cálix, Ana Marques, João Cardoso, Madalena Emiliano, Rita Gerardo, Carlos Nunes, Rita Câmara, José Alberto Ferreira, Aurora Carvalho, Paulo Freitas, Ricardo Cruz‐Correia, João Fonseca,
We aimed to compare patient's and physician's ratings of inhaled medication adherence and to identify predictors of patient-physician discordance.Baseline data from two prospective multicentre observational studies.29 allergy, pulmonology and paediatric secondary care outpatient clinics in Portugal.395 patients (≥13 years old) with persistent asthma.Data on demographics, patient-physician relationship, upper airway control, asthma control, asthma treatment, forced expiratory volume in one second (FEV1) ...
Tópico(s): Cystic Fibrosis Research Advances
2019 - BMJ | BMJ Open
Produção Nacional
Revisado por pares
Aline Rogéria Freire de Castilho, Cristiane Duque, Thaís de Cássia Negrini, Nancy Tomoko Sacono, Andréia Bolzan de Paula, Patrícia Almada Sacramento, Carlos Alberto de Souza Costa, Denise Madalena Palomari Spolidório, Regina Maria Puppin‐Rontani,
To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline hyclate. The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments – agar diffusion test for 24 h and biofilm assay for 24 h and 7 days – against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 μL of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. ...
Tópico(s): Oral microbiology and periodontitis research
2011 - Elsevier BV | Archives of Oral Biology
Nuno Bettencourt, João Rocha, Nuno Ferreira, Gustavo Pires‐Morais, Mónica Carvalho, Daniel Leite, Bruno Melica, Lino Santos, Alberto Rodrigues, Pedro Braga, Madalena Teixeira, Lino Simões, Adelino Leite‐Moreira, Silva Cardoso, Eike Nagel, Vasco Gama,
Background Preliminary studies have shown the potential of myocardial computed tomography perfusion (CTP) analysis for ischemia detection in both animals and humans. Objective To provide validation data on stress-rest CTP protocols as additive tools to improve the accuracy of multidetector computed tomography (MDCT) for coronary artery disease (CAD) in symptomatic patients. Methods Ninety symptomatic patients with suspected CAD (62 ± 8 years, 66% males) underwent both MDCT and invasive coronary angiography ( ...
Tópico(s): Advanced X-ray and CT Imaging
2011 - Elsevier BV | Journal of cardiovascular computed tomography
Produção Nacional
Revisado por pares
Aline Rogéria Freire de Castilho, Cristiane Duque, Thaís de Cássia Negrini, Nancy Tomoko Sacono, Andréia Bolzan de Paula, Carlos Alberto de Souza Costa, Denise Madalena Palomari Spolidório, Regina Maria Puppin‐Rontani,
To evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT). For the in vitro studies, RMGIC was associated with 0.2, 0.5, 1.25 and 2.5% CHX. Microbiologic evaluation consisted of an agar diffusion test on cariogenic ...
Tópico(s): Oral microbiology and periodontitis research
2012 - Elsevier BV | Journal of Dentistry
Nuno Bettencourt, Amedeo Chiribiri, Andreas Schuster, Nuno Ferreira, Francisco Sampaio, Ricardo Duarte, Lino Santos, Bruno Melica, Alberto Rodrigues, Pedro Braga, Madalena Teixeira, Lino Simões, Adelino Leite‐Moreira, José Silva‐Cardoso, Eike Nagel, Pedro Portugal, Vasco Gama,
Tópico(s): Coronary Interventions and Diagnostics
2012 - Elsevier BV | International Journal of Cardiology
Nuno Bettencourt, Nuno Ferreira, Amedeo Chiribiri, Andreas Schuster, Francisco Sampaio, Lino Santos, Bruno Melica, Alberto Rodrigues, Pedro Braga, Madalena Teixeira, Adelino Leite‐Moreira, José Silva‐Cardoso, Pedro Portugal, Vasco Gama, Eike Nagel,
Cardiovascular magnetic resonance (CMR) myocardial perfusion imaging (MPI) is a state-of-the-art noninvasive modality for detection of myocardial ischemia and coronary artery disease. Magnetic resonance coronary angiography (MRCA) allows visualization of the coronary tree, but its incremental value as part of a CMR protocol including MPI and late gadolinium enhancement (LGE) is not well established. We aimed to evaluate the additive diagnostic value of a 3-dimensional whole-heart MRCA integration into ...
Tópico(s): Coronary Interventions and Diagnostics
2013 - Lippincott Williams & Wilkins | Circulation Cardiovascular Imaging
Produção Nacional
Revisado por pares
Maria Luísa Leite, Diana Gabriela Soares, Carla Caroline de Oliveira Duque, Ester Alves Ferreira Bordini, Giovana Anovazzi, Fernanda Gonçalves Basso, Denise Madalena Palomari Spolidório, Josimeri Hebling, Carlos Alberto de Souza Costa,
Tópico(s): Dental Erosion and Treatment
2018 - Springer Nature | Clinical Oral Investigations
Fernando Ribeiro, Alberto J. Alves, Madalena Teixeira, Fátima Miranda, Cristina Azevedo, José Alberto Duarte, José A. Puppim de Oliveira,
Heart rate recovery, defined as the fall in heart rate during the first minute after exercise, is an indicator of autonomic function, and has been found to be an independent predictor of mortality after acute myocardial infarction. Exercise training has several well-known benefits in terms of cardiorespiratory fitness, modifiable cardiovascular risk factors and prognosis after acute coronary events. However, there are no randomized controlled studies in the literature evaluating the effects of exercise ...
Tópico(s): Heart rate and cardiovascular health
2012 - Elsevier BV | Revista Portuguesa de Cardiologia (English Edition)
Madalena Pires da Fonseca, Diana Dias, Carla Sá, Alberto Amaral,
Access to higher education in P ortugal is governed by a quota system ( numerus clausus ). The purpose of this article is to answer two questions: First, how does the numerus clausus system shape the demand for medical studies? Second, how do non‐enrolled medical candidates influence the global allocation system and generate waves of dissatisfaction? The results show that students who do not succeed in entering medicine register in other programmes, such as pharmacy, veterinary medicine and dental studies, ...
Tópico(s): Dental Education, Practice, Research
2013 - Wiley | European Journal of Education
Pedro Antunes, Ana Joaquim, Francisco Sampaio, Célia Nunes, António Ascensão, Eduardo M. Vilela, Madalena Teixeira, Andreia Capela, Anabela Amarelo, C Marques, Sofia Viamonte, Alberto J. Alves, Dulce Esteves,
Abstract Aims Exercise training has been suggested to prevent anthracycline-related cardiac dysfunction, but clinicalbased evidence is scarce. We investigated the effects of a supervised exercise training programme (SETP) on cardiac toxicity markers in women with breast cancer (BC) receiving anthracycline-containing chemotherapy. Methods and results Ninety-three women with early-stage breast cancer were randomly allocated to a supervised exercise training programme (SETP) plus usual care group (Exercise, ...
Tópico(s): Cardiac Imaging and Diagnostics
2023 - Oxford University Press | European Journal of Preventive Cardiology