Alexander Schulte, Hauke S. Günther, Heidi Phillips, Dirk Kemming, Tobias Martens, Samir Kharbanda, Robert Soriano, Zora Modrušan, Svenja Zapf, Manfred Westphal, Katrin Lamszus,
Abstract Glioblastomas contain stem‐like cells that can be maintained in vitro using specific serum‐free conditions. We investigated whether glioblastoma stem‐like (GS) cell lines preserve the expression phenotype of human glioblastomas more closely than conventional glioma cell lines. Expression profiling revealed that a distinct subset of GS lines, which displayed a full stem‐like phenotype (GSf), mirrored the expression signature of glioblastomas more closely than either other GS lines or cell ...
Tópico(s): MicroRNA in disease regulation
2011 - Wiley | Glia
Kimio Yoshizato, Svenja Zapf, Manfred Westphal, Michael E. Berens, Alf Giese,
OBJECTIVE Because of the wide dissemination of malignant glioma cells by the time that malignant glioma is diagnosed, anti-invasive strategies that are designed to limit their further spread may be of little value unless mechanisms of the invasive cascade can be used to render invasive cells susceptible to cytoreductive treatments. We recently determined that elevated thromboxane synthase gene expression and enzymatic activity are associated with a highly migratory phenotype of glioma cells in vitro ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2002 - Lippincott Williams & Wilkins | Neurosurgery
Patrick Janson, Kristina Willeke, Lisa Zaibert, Andrea Budnick, Anne Berghöfer, Sarah Kittel‐Schneider, Peter U. Heuschmann, Andreas Zapf, Manfred Wildner, Carolin Stupp, Thomas Keil,
A systematic overview of mental and physical disorders of informal caregivers based on population-based studies with good methodological quality is lacking. Therefore, our aim was to systematically summarize mortality, incidence, and prevalence estimates of chronic diseases in informal caregivers compared to non-caregivers. Following PRISMA recommendations, we searched major healthcare databases (CINAHL, MEDLINE and Web of Science) systematically for relevant studies published in the last 10 years ( ...
Tópico(s): Family Caregiving in Mental Illness
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Kristina Willeke, Patrick Janson, Katharina Zink, Carolin Stupp, Sarah Kittel‐Schneider, Anne Berghöfer, Thomas Ewert, Ryan King, Peter U. Heuschmann, Andreas Zapf, Manfred Wildner, Thomas Keil,
We aimed to systematically identify and evaluate all studies of good quality that compared the occurrence of mental disorders in the self-employed versus employees. Adhering to the Cochrane guidelines, we conducted a systematic review and searched three major medical databases (MEDLINE, Web of Science, Embase), complemented by hand search. We included 26 (three longitudinal and 23 cross-sectional) population-based studies of good quality (using a validated quality assessment tool), with data from 3, ...
Tópico(s): Fibromyalgia and Chronic Fatigue Syndrome Research
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Carmen Eckerich, Alexander Schulte, Tobias Martens, Svenja Zapf, Manfred Westphal, Katrin Lamszus,
Malignant gliomas are incurable because of their diffuse infiltration of the surrounding brain. The recepteur d'origine nantais (RON) receptor tyrosine kinase is highly expressed in several epithelial cancer types and mediates tumorigenic, pro-invasive as well as metastatic effects. Analyzing RON expression in human gliomas, we found that different splice variants with known oncogenic activity are expressed in glioblastomas (GBM). In addition, the RON ligand macrophage-stimulating protein (MSP) is ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2009 - Wiley | Journal of Neurochemistry
Gabriele Bolte, A. Heißenhuber, Rüdiger von Kries, Bernhard Liebl, Andreas Zapf, Manfred Wildner, Hermann Fromme,
Tópico(s): School Health and Nursing Education
2007 - Springer Science+Business Media | Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz
E. L. Kim, Kimio Yoshizato, Lan Kluwe, Hildegard Meissner, Gabriele Warnecke, S. Zapf, Manfred Westphal, Wolfgang Deppert, Armin Giese,
Background: p53 is the most frequently mutated gene in human cancers and its functional integrity is an important predictor of treatment response and clinical outcome. The majority of mutations found in different types of cancer cluster within the DNA binding domain encoded by exons 5-8. In clinical specimens the functional status of p53 is, therefore, often evaluated by direct mutation analysis of exons 5-8 or indirectly by immunostaining and evaluation of the subcellular localization pattern or ...
Tópico(s): Microtubule and mitosis dynamics
2005 - International Institute of Anticancer Research (IIAR) Conferences 1997. Athens, Greece. Abstracts | Anticancer Research
Kimio Yoshizato, Svenja Zapf, Manfred Westphal, Michael E. Berens, Alf Giese,
Tópico(s): Eicosanoids and Hypertension Pharmacology
2002 - Lippincott Williams & Wilkins | Neurosurgery
F. Kürzel, C Hagel, S. Zapf, Hildegard Meissner, Manfred Westphal, Armin Giese,
Tópico(s): Estrogen and related hormone effects
2002 - Springer Science+Business Media | Acta Neurochirurgica
Malte Mohme, Cécile L. Maire, Kristoffer Riecken, Svenja Zapf, Tim Aranyossy, Manfred Westphal, Katrin Lamszus, Boris Fehse,
Intratumoral heterogeneity has been identified as one of the strongest drivers of treatment resistance and tumor recurrence. Therefore, investigating the complex clonal architecture of tumors over time has become a major challenge in cancer research. We developed a new fluorescent "optical barcoding" technique that allows fast tracking, identification, and quantification of live cell clones in vitro and in vivo using flow cytometry (FC). We optically barcoded two cell lines derived from malignant ...
Tópico(s): CRISPR and Genetic Engineering
2017 - Elsevier BV | Molecular Therapy
Maximilian J. A. Puchner, Armin Giese, S. Zapf, Markus Grebe, Manfred Westphal,
Tópico(s): Computational Drug Discovery Methods
2001 - Springer Science+Business Media | Acta Neurochirurgica
Alexander Schulte, Katrin Liffers, Annegret Kathagen, Sabine Riethdorf, Svenja Zapf, Adrian Merlo, Katharina Kolbe, Manfred Westphal, Katrin Lamszus,
Background.The treatment efficacy of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors like erlotinib has not met expectations for glioblastoma therapy, even for EGFR-overexpressing tumors.We determined possible mechanisms of therapy resistance using the unique BS153 glioblastoma cell line, which has retained amplification of the egfr gene and expression of EGFR variant (v)III.Methods.Functional effects of erlotinib, gefitinib, and cetuximab on BS153 proliferation, migration, and ...
Tópico(s): Colorectal Cancer Treatments and Studies
2013 - Oxford University Press | Neuro-Oncology
N. Derichs, Javier Sanz, Thomas von Känel, C. Stolpe, Antonia Zapf, B. Tümmler, Sabina Gallati, Manfred Ballmann,
The value of the signs and symptoms of pregnancy and of the pregnancy diagnostic test as predictors of pregnancy was estimated for 1,592 women seen in general practice. The presence of a sign or symptom alone is a poor pointer to a diagnosis of pregnancy. The combination of pairs of features improves the value, the best being breast signs combined with either the presence of signs of pregnancy on vaginal examination or a palpable fundus, both giving predictive values of 0·89. The pregnancy diagnostic ...
Tópico(s): Esophageal and GI Pathology
2010 - BMJ | Thorax
Carmen Eckerich, Svenja Zapf, Regina Fillbrandt, Sonja Loges, Manfred Westphal, Katrin Lamszus,
The c-Met receptor and its ligand scatter factor/hepatocyte growth factor (SF/HGF) are strongly overexpressed in malignant gliomas. Signaling through c-Met as well as exposure to hypoxia can stimulate glioma cell migration and invasion. In several cancer cell types, hypoxia was shown to activate the c-met promoter, which contains hypoxia inducible factor-1 (HIF-1) binding sites. We hypothesized that hypoxia might upregulate c-Met also in glioma cells. Analyzing 18 different glioblastoma cell lines ...
Tópico(s): Organ Transplantation Techniques and Outcomes
2007 - Wiley | International Journal of Cancer
Alf Giese, Christian Hagel, Ella L. Kim, Svenja Zapf, Jasmin Djawaheri, Michael E. Berens, Manfred Westphal,
The capacity of glial tumor cells to migrate and diffusely infiltrate normal brain compromises surgical eradication of the disease. Identification of genes associated with invasion may offer novel strategies for anti-invasive therapies. The gene for TXsyn, an enzyme of the arachidonic acid pathway, has been identified by differential mRNA display as being overexpressed in a glioma cell line selected for migration. In this study TXsyn mRNA expression was found in a large panel of glioma cell lines ...
Tópico(s): Estrogen and related hormone effects
1999 - Oxford University Press | Neuro-Oncology
Elżbieta Kaczmarek, Svenja Zapf, Hakim Bouterfa, Jörg C. Tonn, Manfred Westphal, Alf Giese,
Abstract The invasive cellular behavior of malignant gliomas is determined by receptor mediated cell–substratum contacts and cell–cell interaction as well as cellular locomotion. This study attempts to break down the complex phenomena of the invasive process into their components of attachment to neighboring cells, aggregate formation, adhesion to matrix substratum, migration and invasion into three‐dimensional cellular aggregates separately analyzed in different in vitro assay systems. Using a ...
Tópico(s): Cell Adhesion Molecules Research
1999 - Wiley | International Journal of Developmental Neuroscience
Alexander Schulte, Hauke S. Günther, Tobias Martens, Svenja Zapf, Sabine Riethdorf, Clemens Wülfing, Malgorzata Stoupiec, Manfred Westphal, Katrin Lamszus,
Abstract Purpose: Despite the high incidence of epidermal growth factor receptor (EGFR) gene amplification and rearrangement in glioblastomas, no suitable cell line exists that preserves these alterations in vitro and is tumorigenic in immunocompromised mice. On the basis of previous observations that glioblastoma cells cultured with serum lose the EGFR amplification rapidly and that EGF can inhibit the growth of EGFR-amplified tumor cells, we hypothesized that serum-free and EGF-free culture conditions ...
Tópico(s): Mathematical Biology Tumor Growth
2012 - American Association for Cancer Research | Clinical Cancer Research
Carmen Eckerich, Svenja Zapf, Ulrike Ulbricht, Sabine Müller, Regina Fillbrandt, Manfred Westphal, Katrin Lamszus,
Abstract Contactin is a cell surface adhesion molecule that is normally expressed by neurons and oligodendrocytes. Particularly high levels of contactin are present during brain development. Using subtractive cloning, we identified contactin transcripts as overexpressed in glioblastomas compared with normal brain. We confirmed contactin overexpression in glioblastomas at the protein level, and localized contactin to the surface of glial fibrillary acidic protein (GFAP)‐expressing glioblastoma cells. ...
Tópico(s): Cell Adhesion Molecules Research
2005 - Wiley | Glia
Matthias Lukasczik, Jutta Ahnert, Veronika Ströbl, Heiner Vogel, Carolin Donath, Ilka M. Enger, Elmar Gräßel, Lena Heyelmann, Heidemarie Lux, Jochen Maurer, Dominik Özbe, Stefanie Spieckenbaum, Elzbieta Voigtländer, Manfred Wildner, Andreas Zapf, A. Zellner, Alfons Hollederer,
Healthcare professionals are confronted with specific work-related demands that influence work-family relations and might indirectly affect the quality of healthcare. This paper seeks to provide an overview of the current state of research on this topic of relevance to health services research. The overview may serve as a starting point for modifying structures in the healthcare system (especially in rural regions) with the aim of improving work-family compatibility.A systematic national and international ...
Tópico(s): Workplace Health and Well-being
2017 - Thieme Medical Publishers (Germany) | Das Gesundheitswesen
Cécile L. Maire, Malte Mohme, Michael Bockmayr, Krystian Fita, Kristoffer Riecken, Daniela Börnigen, Malik Alawi, Antonio Virgilio Failla, Katharina Kolbe, Svenja Zapf, Mareike Holz, Katrin Neumann, Lasse Dührsen, Tobias Lange, Boris Fehse, Manfred Westphal, Katrin Lamszus,
Immunotherapeutic strategies are increasingly important in neuro-oncology, and the elucidation of escape mechanisms that lead to treatment resistance is crucial. We investigated the impact of immune pressure on the clonal dynamics and immune escape signature by comparing glioma growth in immunocompetent versus immunodeficient mice. Glioma-bearing WT and Pd-1-/- mice survived significantly longer than immunodeficient Pfp-/- Rag2-/- mice. While tumors in Pfp-/- Rag2-/- mice were highly polyclonal, ...
Tópico(s): Glioma Diagnosis and Treatment
2020 - American Society for Clinical Investigation | Journal of Clinical Investigation
Malte Mohme, Simon Schliffke, Cécile L. Maire, Alessandra Rünger, Laura Glau, Klaus C. Mende, Jakob Matschke, Christina Gehbauer, Nuray Akyüz, Svenja Zapf, Mareike Holz, Miriam Schaper, Tobias Martens, Nils Ole Schmidt, Sven Peine, Manfred Westphal, Mascha Binder, Eva Tolosa, Katrin Lamszus,
Purpose: Immunotherapeutic treatment strategies for glioblastoma (GBM) are under investigation in clinical trials. However, our understanding of the immune phenotype of GBM-infiltrating T cells (tumor-infiltrating lymphocytes; TILs) and changes during disease progression is limited. Deeper insight is urgently needed to therapeutically overcome tumor-induced immune exhaustion.Experimental Design: We used flow cytometry and cytokine assays to profile TILs and peripheral blood lymphocytes (PBLs) from ...
Tópico(s): Immunotherapy and Immune Responses
2018 - American Association for Cancer Research | Clinical Cancer Research
Gabriele Bolte, G. Büchele, U Schwegler, E Roscher, Andreas Zapf, Manfred Wildner, Hermann Fromme,
Ziel: Das Ziel der Studie war die systematische und standardisierte Erhebung der Prävalenz von Atemwegserkrankungen bei Kindern in einem ländlichen Gebiet in Bayern, um zu klären, ob ein Erkrankungs-Cluster vorliegt. Anlass für die Untersuchung waren Berichte einer Elterninitiative über eine Häufung von Erkrankungen, für die ein Zusammenhang mit einer erhöhten Luftschadstoffbelastung durch benachbarte Betriebe und den damit verbundenen Kraftfahrzeugverkehr vermutet wurde. Methoden: Es wurde eine ...
Tópico(s): Indoor Air Quality and Microbial Exposure
2006 - Thieme Medical Publishers (Germany) | Das Gesundheitswesen
Andreas Müller, Klaus Stefan Drese, Hubert Gnaser, Manfred J. Hampe, Volker Hessel, Holger Löwe, Stefan Schmitt, Ralf Zapf,
Heterogeneous gas phase catalyst screening with catalysts applied on titer-plates is presented. It will be shown that titer-plates can as well be used for the sample preparation outside the reactor and for the catalyst testing in the reactor. For the catalyst preparation, the known wash-coating-impregnation procedure is applied. An alumina coated precursor is introduced into a manifold which utilizes the flow features of the separated wells for the impregnation of the precursor with solutions of ...
Tópico(s): Catalysis and Oxidation Reactions
2003 - Elsevier BV | Catalysis Today
Kristina Willeke, Patrick Janson, Katharina Zink, Christina Tischer, Peter U. Heuschmann, Andreas Zapf, Manfred Wildner, Carolin Stupp, Thomas Keil,
BACKGROUND: A stringent systematic review of population-based observational studies focusing on the physical health of self-employed individuals as a basis for the development of targeted prevention strategies is lacking. OBJECTIVE: We aimed to systematically evaluate all the studies of good quality that compared the occurrence of chronic physical disorders in self-employed individuals with that of employees. METHODS: We searched three major medical databases (MEDLINE, Web of Science, Embase) following ...
Tópico(s): Occupational exposure and asthma
2023 - IOS Press | Work
Alf Giese, Christian Hagel, Ella L. Kim, Svenja Zapf, Jasmin Djawaheri, Michael E. Berens, Manfred Westphal,
The capacity of glial tumor cells to migrate and diffusely in ltrate normal brain compromises surgical eradication of the disease.Identi cation of genes associated with invasion may offer novel strategies for anti-invasive therapies.The gene for TXsyn, an enzyme of the arachidonic acid pathway, has been identi ed by differential mRNA display as being overexpressed in a glioma cell line selected for migration.In this study TXsyn mRNA expression was found in a large panel of glioma cell lines but ...
Tópico(s): Chemical Synthesis and Analysis
1999 - Oxford University Press | Neuro-Oncology
Alfons Hollederer, G. Braun, G. Dahlhoff, Hans Drexler, John D. Engel, Elmar Gräßel, Eveline Häusler, Homan van der Heide, Peter U. Heuschmann, G. Hörl, H. Imhof, Kaplan Ma, R. Kasperbauer, David Klemperer, Peter L. Kolominsky‐Rabas, Joseph Kuhn, Markus Lang, R. Langejürgen, A. Lankes, Reiner Leidl, Bernhard Liebl, Julika Loss, Kristin Ludewig, Ulrich Mansmann, N. Melcop, Klaus Nagels, Dennis Nowak, H. Pfundner, Bernd Reuschenbach, Armin Schneider, W Schneider, Oliver Schöffski, W. Schreiber, Sven Voigtländer, Manfred Wildner, Andreas Zapf, A. Zellner,
Zweck des Memorandums zur Weiterentwicklung der Gesundheitsversorgungsforschung in Bayern ist es, die globalen Ziele der Landesarbeitsgemeinschaft Gesundheitsversorgungsforschung (LAGeV) zu operationalisieren und gemeinsam auf Basis der Bestandsanalyse Prioritäten hinsichtlich der Zukunftsthemen, konkrete Umsetzungsschritte, Methoden und Arbeitsformen für den weiteren Verlauf festzulegen. Die LAGeV ist ein Fachgremium, das die Kompetenzen von Akteuren aus Wissenschaft, Politik und Praxis im Hinblick ...
Tópico(s): Social and Demographic Issues in Germany
2014 - Thieme Medical Publishers (Germany) | Das Gesundheitswesen
Malte Mohme, Cécile L. Maire, Kristoffer Riecken, Malik Alawi, Lasse Dührsen, Katrin Neumann, Tobias Lange, Antonio Virgilio Failla, Svenja Zapf, Boris Fehse, Manfred Westphal, Katrin Lamszus,
Given the increasing importance of immunotherapeutic treatment strategies in neuro-oncology, the investigation of immune escape mechanisms, which lead to treatment resistance, is crucial. Therefore, studying tumor heterogeneity to understand the clonal dynamics and emergence of immune escape variants during cancer immunoediting can yield valuable insights into the adaptive processes of treatment resistance. We applied a novel multicolor labeling strategy, termed optical barcoding, which makes use ...
Tópico(s): Genetics, Bioinformatics, and Biomedical Research
2017 - Oxford University Press | Neuro-Oncology
Andreas Zapf, Joseph Kuhn, Manfred Wildner,
Einleitung Die Tätigkeitsfelder des Öffentlichen Gesundheitsdienstes erleben eine zunehmende Annäherung an verschiedenste Public Health Anwendungsfelder. Daraus ergeben sich Perspektiven für eine sowohl inhaltliche als auch methodische gemeinsame Weiterentwicklung von ÖGD und Public Health im 21. Jahrhundert.
Tópico(s): Medical and Health Sciences Research
2014 - De Gruyter | Public Health Forum
Patrick Janson, Chu-Wei Hung, Kristina Willeke, Dieter Frisch, Anne Berghöfer, Peter U. Heuschmann, Andreas Zapf, Manfred Wildner, Carolin Stupp, Thomas Keil,
Zusammenfassung Einleitung Die Pflege eines Angehörigen ist mit psychischen Erkrankungen und verminderter Lebensqualität der Pflegenden assoziiert. Das Ziel dieser systematischen Übersichtsarbeit war es, die Ergebnisse methodisch guter Interventionsstudien zur Wirksamkeit nicht-pharmakologischer Interventionen auf patientenrelevante Endpunkte bei pflegenden Angehörigen in Deutschland zusammenzufassen. Methodik In drei großen wissenschaftlichen Literaturdatenbanken suchten wir nach Interventionsstudien ...
Tópico(s): Intergenerational Family Dynamics and Caregiving
2024 - Thieme Medical Publishers (Germany) | Das Gesundheitswesen
Kristina Willeke, Patrick Janson, Anna Kirchner, Christina Tischer, Arun D’Souza, Peter U. Heuschmann, Andreas Zapf, Manfred Wildner, Carolin Stupp, Thomas Keil,
BACKGROUND: Compared to workers of larger companies it is less clear what health promoting interventions might be beneficial for employees of small businesses and self-employed individuals. OBJECTIVE: Our aim was to critically appraise trials investigating health promotion programs among small business workers and self-employed individuals, by means of a systematic review. METHODS: We conducted a search of primary studies using MEDLINE, Web of Science, LIVIVO and the Cochrane library. Our assessment ...
Tópico(s): Climate Change and Health Impacts
2024 - IOS Press | Work