Diana Tomás, Joana Rodrigues, Ana Paula Muterle Varela, Manuela Veloso, Wanda Viegas, Manuela Silva,
Genomic diversity of Portuguese accessions of Avena species--diploid A. strigosa and hexaploids A. sativa and A. sterilis--was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species--rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies--IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite ...
Tópico(s): Plant Pathogens and Resistance
2016 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Hugo R. Oliveira, Diana Tomás, Manuela Silva, Susana Lopes, Wanda Viegas, Manuela Veloso,
Faba bean (Vicia faba L.) is a facultative cross-pollinating legume crop with a great importance for food and feed due to its high protein content as well as the important role in soil fertility and nitrogen fixation. In this work we evaluated genetic diversity and population structure of faba bean accessions from the Western Mediterranean basin and wild related species. For that purpose we screened 53 V. faba, 2 V. johannis and 7 V. narbonensis accessions from Portugal, Spain and Morocco with 28 ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
2016 - Public Library of Science | PLoS ONE
Miguel Bento, H. Sofia Pereira, Margarida Rocheta, Perry Gustafson, Wanda Viegas, Manuela Silva,
BackgroundPolyploidization is a major evolutionary process in plants where hybridization and chromosome doubling induce enormous genomic stress and can generate genetic and epigenetic modifications. However, proper evaluation of DNA sequence restructuring events and the precise characterization of sequences involved are still sparse.Methodology/Principal FindingsInter Retrotransposons Amplified Polymorphism (IRAP), Retrotransposons Microsatellite Amplified Polymorphism (REMAP) and Inter Simple Sequence ...
Tópico(s): Plant Disease Resistance and Genetics
2008 - Public Library of Science | PLoS ONE
Miguel Bento, Perry Gustafson, Wanda Viegas, Manuela Silva,
Genetic and epigenetic modifications resulting from different genomes adjusting to a common nuclear environment have been observed in polyploids. Sequence restructuring within genomes involving retrotransposon/microsatellite-rich regions has been reported in triticale. The present study uses inter-retrotransposon amplified polymorphisms (IRAP) and retrotransposon microsatellite amplified polymorphisms (REMAP) to assess genome rearrangements in wheat–rye addition lines obtained by the controlled backcrossing ...
Tópico(s): Plant Disease Resistance and Genetics
2010 - Springer Science+Business Media | Theoretical and Applied Genetics
Miguel Bento, J. P. Gustafson, Wanda Viegas, Manuela Silva,
Polyploidization is one of the major driving forces in plant evolution and is extremely relevant to speciation and diversity creation. Polyploidization leads to a myriad of genetic and epigenetic alterations that ultimately generate plants and species with increased genome plasticity. Polyploids are the result of the fusion of two or more genomes into the same nucleus and can be classified as allopolyploids (different genomes) or autopolyploids (same genome). Triticeae synthetic allopolyploid species ...
Tópico(s): Plant Disease Resistance and Genetics
2011 - NRC Research Press | Genome
Álvaro Antônio Alencar de Queiroz, Augusto Assunção, Ilda Ramadas, Wanda Viegas, Manuela Veloso,
A very rich heritage of pear (Pyrus communis) landraces is still maintained in Portugal. In this work, we present the molecular characterization of 48 accessions from the national reference collection at Vidago and Felgueiras. The core set of six microsatellite markers recommended by the European Cooperative Programme for Plant Genetic Resources was used. Thirty-six distinct genotypes were scored since several synonymies were found and three homonymous cultivars were detected. Three cultivars were ...
Tópico(s): Plant Physiology and Cultivation Studies
2014 - Elsevier BV | Scientia Horticulturae
Miguel Bento, Diana Tomás, Wanda Viegas, Manuela Silva,
Understanding how increased genome size and diversity within polyploid genomes impacts plant evolution and breeding continues to be challenging. Although historical studies by McClintock suggested the importance of transposable elements mediated by polyploidisation on genomic changes, data from plant crosses remain scarce. Despite the absence of a conclusive proof regarding autonomous retrotransposon movement in synthetic allopolyploids, the transposition of retrotransposons and their ubiquitous ...
Tópico(s): Plant tissue culture and regeneration
2013 - Karger Publishers | Cytogenetic and Genome Research
M. Cecília Almadanim, Margarida Baleiras-Couto, H. Sofia Pereira, L. C. Carneiro, Pedro Fevereiro, José Eiras‐Dias, Leonor Morais‐Cecílio, Wanda Viegas, Manuela Veloso,
Portugal is very rich in grapevine biodiversity but nowadays only a small number of cultivars (13 black and 3 white) with defined enological characteristics are being used. However, there are still 51 cultivars with significant expression in the country which have a great potential to be used in the establishment of new vineyards. Considering the importance of identifying and understanding the genetic relatedness of the main cultivars used for wine making in Portugal, those cultivars were genotyped ...
Tópico(s): Wine Industry and Tourism
2015 - Julius Kühn-Institut | Vitis
Álvaro Antônio Alencar de Queiroz, Joana Bagoin Guimarães, Claudia Sánchez, Fernanda Simões, Rui Maia Sousa, Wanda Viegas, Manuela Veloso,
A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, “Rocha” dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as to date molecular studies have been performed on a single “Rocha” clone. Eleven Simple Sequence Repeats (SSR) markers were used to assess the genetic diversity of 130 local cultivars, 80 of them being “Rocha” ...
Tópico(s): Plant Pathogens and Resistance
2019 - Multidisciplinary Digital Publishing Institute | Sustainability
N. F. Ramos, Margarida S. Miranda, Albina R. Franco, Simone S. Silva, Jorge Manuel Teixeira de Azevedo, Isabel R. Dias, Rui L. Reis, Carlos Viegas, Manuela E. Gomes,
Silk has been used in several biomedical applications, including tissue engineering, drug delivery systems, biomedical implants, and diagnostic medical devices, due to its attractive intrinsic properties such as biocompatibility, controllable biodegradability, and excellent mechanical properties. In recent years, several attempts have been made to produce silk fibers with improved properties and new functionalities by feeding silkworms with modified diets containing nanomaterials. Nanomaterials ...
Tópico(s): Insect Utilization and Effects
2020 - American Chemical Society | ACS Sustainable Chemistry & Engineering
Diana Tomás, José Carlos Rodrígues, Wanda Viegas, Manuela Silva,
Wheat is one of the most important cereals for food and feed, and it is, therefore, necessary to determine the effects of short-term high temperature events (heatwaves) during grain filling. These heatwave events are increasingly common, especially in Portugal. In this work, seven commercial varieties recommended for production in Portugal were submitted to one-week high temperature (HT) treatment ten days after anthesis to evaluate heat effects on grain yield and quality. Grain yield parameters, ...
Tópico(s): Meat and Animal Product Quality
2020 - Multidisciplinary Digital Publishing Institute | Agronomy
Diana Tomás, Ana Paula Soares Dias, Manuela Silva, Hugo R. Oliveira, María José Suso, Wanda Viegas, Manuela Veloso,
Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. ...
Tópico(s): Agricultural pest management studies
2016 - Multidisciplinary Digital Publishing Institute | Diversity
Diana Tomás, João Brazão, Wanda Viegas, Manuela Silva,
The plant stress response has been extensively characterized at the biochemical and physiological levels. However, knowledge concerning repetitive sequence genome fraction modulation during extreme temperature conditions is scarce. We studied high-temperature effects on subtelomeric repetitive sequences (pSc200) and 45S rDNA in rye seedlings submitted to 40°C during 4 h. Chromatin organization patterns were evaluated through fluorescent in situ hybridization and transcription levels were assessed ...
Tópico(s): RNA Research and Splicing
2012 - Karger Publishers | Cytogenetic and Genome Research
Joana Rodrigues, Wanda Viegas, Manuela Silva,
The genus Avena comprises four distinct genomes organized in diploid (AA or CC), tetraploid (AABB or AACC) and hexaploid species (AACCDD), constituting an interesting model for phylogenetic analysis. The aim of this work was to characterize 45S rDNA intergenic spacer (IGS) variability in distinct species representative of Avena genome diversity-A. strigosa (AA), A. ventricosa (CvCv), A. eriantha (CpCp), A. barbata (AABB), A. murphyi (AACC), A. sativa (AACCDD) and A. sterilis (AACCDD) through the assessment ...
Tópico(s): Genomics and Phylogenetic Studies
2017 - Public Library of Science | PLoS ONE
Diana Tomás, Wanda Viegas, Manuela Silva,
Wheat is undoubtedly one of the most important crops worldwide and it is essential to study how the distinct varieties answer to heat waves associated with climatic changes, in order to design adequate wheat breeding strategies. To assess high temperature (HT) impact in wheat grain characteristics, seven commercial varieties, which have been recommended for production in Portugal, were submitted for one-week HT treatment ten days after anthesis. Firstly, predicted grain technological quality was ...
Tópico(s): Crop Yield and Soil Fertility
2020 - Multidisciplinary Digital Publishing Institute | Agronomy
Cristina P. Sousa, Fabiano G. Nery, Jorge Manuel Teixeira de Azevedo, Carlos Viegas, Manuela E. Gomes, Isabel R. Dias,
Values of serum tartrate-resistant acid phosphatase ( TRAP) activity were obtained in adult dogs and its biological variability was assessed. Nine healthy skeletally mature Portuguese Podengo dogs were used for the determination of TRAP, total and bone alkaline phosphatase serum activities, and also to study their relationship with serum minerals, namely calcium (Ca), phosphorous (P), and magnesium (Mg). The serum TRAP activity was 2.19±0.56IU/mL, with intra-individual variation of 18.3% and inter-individual ...
Tópico(s): Bone health and osteoporosis research
2011 - UNIVERSIDADE FEDERAL DE MINAS GERAIS | Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Diana Tomás, Luís Coelho, José Carlos Rodrígues, Wanda Viegas, Manuela Silva,
Wheat is a dietary staple consumed worldwide strongly responsible for proteins and carbohydrate population intake. However, wheat production and quality will scarcely fulfill forward demands, which are compounded by high-temperature (HT) events as heatwaves, increasingly common in Portugal. Thus, landraces assume crucial importance as potential reservoirs of useful traits for wheat breeding and may be pre-adapted to extreme environmental conditions. This work evaluates four Portuguese landrace yield ...
Tópico(s): Crop Yield and Soil Fertility
2020 - Frontiers Media | Frontiers in Plant Science
Miguel Bento, Sónia Gomes Pereira, Wanda Viegas, Manuela Silva,
The predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. It is therefore vital to understand crops diversity associated to abiotic stress response. Heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for a precocious and rigorous evaluation of varietal diversity regarding heat tolerance. Plant cell membrane thermostability (CMS) is a widely used method ...
Tópico(s): Plant Water Relations and Carbon Dynamics
2017 - Public Library of Science | PLoS ONE
Diana Tomás, Wanda Viegas, Manuela Silva,
High temperature (HT) events have negative impact on wheat grains yield and quality. Transcriptome profiles of wheat developing grains of commercial genotypes (Antequera and Bancal) and landraces (Ardito and Magueija) submitted to heatwave-like treatments during grain filling were evaluated. Landraces showed significantly more differentially expressed genes (DEGs) and presented more similar responses than commercial genotypes. DEGs were more associated with transcription and RNA and protein synthesis ...
Tópico(s): Wheat and Barley Genetics and Pathology
2022 - Frontiers Media | Frontiers in Plant Science
José Araújo-Martins, Pedro Martins, J. Viegas, Daniel Aelenei, Manuela Cano, João Paulo Teixeira, Paulo Paixão, Ana Luísa Papoila, Paula Pinto, Catarina Pedro, J. Rosado‐Pinto, Isabella Annesi‐Maesano, Nuno Neuparth,
Indoor air quality (IAQ) is considered an important determinant of human health. The association between exposure to volatile organic compounds, particulate matter, house dust mite, molds and bacteria in day care centers (DCC) is not completely clear. The aim of this project was to study these effects. This study comprised two phases. Phase I included an evaluation of 45 DCCs (25 from Lisbon and 20 from Oporto, targeting 5161 children). In this phase, building characteristics, indoor CO2 and air temperature/ ...
Tópico(s): Air Quality and Health Impacts
2014 - Elsevier BV | Revista Portuguesa de Pneumologia
Márcia T. Rodrigues, Albino Martins, Isabel R. Dias, Carlos Viegas, Nuno M. Neves, Manuela E. Gomes, Rui L. Reis,
Bone extracellular matrix (ECM) is composed of mineralized collagen fibrils which support biological apatite nucleation that participates in bone outstanding properties. Understanding and mimicking bone morphological and physiological parameters at a biological scale is a major challenge in tissue engineering scaffolding. Using emergent (nano)technologies scaffold designing may be critically improved, enabling highly functional tissue substitutes for bone applications. This study aims to develop novel ...
Tópico(s): biodegradable polymer synthesis and properties
2012 - Wiley | Journal of Tissue Engineering and Regenerative Medicine
Elena G. Popa, Pedro P. Carvalho, Ana F.V. Dias, T. C. Santos, Vítor Espirito Santo, Alexandra P. Marques, Carlos Viegas, Isabel R. Dias, Manuela E. Gomes, Rui L. Reis,
Carrageenans are highly sulphated galactans, well-known for their thermogelation properties which have been extensively exploited in food and cosmetics industry but poorly explored in the biomedicine field. In this study, we have assessed the in vitro and in vivo biocompatibility of κ-carrageenan hydrogels that have been explored for regenerative medicine and tissue engineering applications. The in vitro cytotoxicity of the materials using a L929 mouse fibroblast cell line was evaluated, and the ...
Tópico(s): Algal biology and biofuel production
2014 - Wiley | Journal of Biomedical Materials Research Part A
Cláudia Marcos, Cláudia Viegas, André M. Almeida, Manuela Guerra,
Traditional sausages—smoked, fermented or dried—are meat products that are part of the traditional daily diet in rural Portugal, and also highly valued in major cities with an increasing demand. These ethnic meat products are manufactured mainly by small-scale industries or artisanal producers according to and/or inspired by traditional processes. They are present in a wide variety of types, many recognized for their quality (38 certified products). Presently, cure technologies used are important ...
Tópico(s): Insect Utilization and Effects
2016 - BioMed Central | Journal of Ethnic Foods
Wanda Viegas, Manuela Silva, Nuno Neves, Alexandra Castilho, Augusta Barão, Álvaro Antônio Alencar de Queiroz, J. S. Heslop‐Harrison, Leonor Morais‐Cecílio, Luis Amado, Margarida Delgado, Vitor Carvalho,
Using the silver staining technique, which allows the identification in metaphase cells of rDNA loci active in the previous interphase, it was observed the consistent expression of 1R NOR in hexaploid triticale cultivars with a 2R/2D chromosome substitution, confirmed through genomic in situ hybridization using rye total genomic DNA and different cloned probes of repetitive sequences. A control mediated by non-nucleolar chromosomes on the suppression of rye NOR in triticale was therefore detected ...
Tópico(s): Plant Disease Resistance and Genetics
1996 - Springer Nature (Netherlands) | Developments in plant breeding
Wanda Viegas, Nathália de Andrade Neves, Manuela Silva, Ana D. Caperta, Leonor Morais‐Cecílio,
Nucleolar dominance is an enigma. The puzzle of differential amphiplasty has remained unresolved since it was first recognised and described in Crepis hybrids by Navashin in 1934. Here we review the body of knowledge that has grown out of the many models that have tried to find the genetic basis for differential rRNA gene expression in hybrids, and present a new interpretation. We propose and discuss a chromatin imprinting model which re-interprets differential amphiplasty in terms of two genomes ...
Tópico(s): Genetic Mapping and Diversity in Plants and Animals
2002 - Bentham Science Publishers | Current Genomics
Isabel R. Dias, José Arthur de Abreu Camassa, João A. Bordelo, Pedro S. Babo, Carlos Viegas, Nuno Dourado, Rui L. Reis, Manuela E. Gomes,
Tópico(s): Bone health and treatments
2018 - Springer Science+Business Media | Current Osteoporosis Reports
Alexandra Castilho, Álvaro Antônio Alencar de Queiroz, Nathália de Andrade Neves, Augusta Barão, Manuela Silva, Wanda Viegas,
Tópico(s): Plant Genetic and Mutation Studies
1995 - Springer Science+Business Media | Chromosome Research
Diana Tomás, Miguel Bento, Wanda Viegas, Manuela Silva,
The union of different genomes in the same nucleus frequently results in hybrid genotypes with improved genome plasticity related to both genome remodeling events and changes in gene expression. Most modern cereal crops are polyploid species. Triticale, synthesized by the cross between wheat and rye, constitutes an excellent model to study polyploidization functional implications. We intend to attain a deeper knowledge of dispersed repetitive sequence involvement in parental genome reshuffle in ...
Tópico(s): Plant Disease Resistance and Genetics
2012 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Miguel Bento, Diana Tomás, Wanda Viegas, Manuela Silva,
Polyploidization is a major genome modification that results in plant species with multiple chromosome sets. Parental genome adjustment to co-habit a new nuclear environment results in additional innovation outcomes. We intended to assess genomic changes in polyploid model species with small genomes using inter retrotransposons amplified polymorphism (IRAP) and retrotransposon microsatellite amplified polymorphism (REMAP). Comparative analysis among diploid and autotetraploid A. thaliana and A. suecica ...
Tópico(s): Plant Virus Research Studies
2015 - Institute of Experimental Botany of the Czech Academy of Sciences | Biologia Plantarum
Márcia T. Rodrigues, Manuela E. Gomes, Carlos Viegas, Jorge Manuel Teixeira de Azevedo, Isabel R. Dias, Fernando Muñóz, Rui L. Reis,
This study aims to assess the in vivo performance of cell-scaffold constructs composed of goat marrow stromal cells (GBMCs) and SPCL (a blend of starch with polycaprolactone) fibre mesh scaffolds at different stages of development, using an autologous model. GBMCs from iliac crests were seeded onto SPCL scaffolds and in vitro cultured for 1 and 7 days in osteogenic medium. After 1 and 7 days, the constructs were characterized for proliferation and initial osteoblastic expression by alkaline phosphatase ( ...
Tópico(s): Periodontal Regeneration and Treatments
2010 - Wiley | Journal of Tissue Engineering and Regenerative Medicine