Produção Nacional
Revisado por pares
Mariana Araya de Godoy, Amanda S. Souza, Mônica Lobo, Omar Vidal Kress Sampaio, Louise Moraes, Marcelo R. Baldanza, Tatiana Magri, João Pedro Saar Wernerck de Castro, Maria das Graças Tavares do Carmo, Márcia Soares-Mota, Mônica S. Rocha, Rosalia Méndez‐Otero, Marcelo F. Santiago,
There is no consensus about the effects of protein restriction on neurogenesis and behavior. Here, for the first time, we evaluated the effects of protein restriction during gestation and lactation, on the two major neurogenic regions of the adult brain, the subgranular zone (SGZ) of the hippocampal dentate gyrus and the subventricular zone (SVZ), simultaneously. We also assessed different types of behavior relevant to each region. After mating, pregnant Wistar rats were divided into a control group ( ...
Tópico(s): Anesthesia and Neurotoxicity Research
2012 - Elsevier BV | Brain Research
María de los Ángeles Fanaro, María Rita Otero, Marcelo Argelo,
This paper is part of a doctoral thesis that investigates Basic Quantum Mechanics (QM) teaching in high school. A Conceptual Structure of Reference (CSR) based on the Path Integral Method of Feynman (1965) was rebuilt and a Proposed Conceptual Structure for Teaching (PCST) (Otero, 2006, 2007) the basics of Quantum Mechanics at secondary school was designed, analysed and carried out. This PCST does not follow the historical route and it is complementary to the canonical formalism. The concepts: probability ...
Tópico(s): Statistics Education and Methodologies
2016 - UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL | Investigações em Ensino de Ciências
Alexánder Carreño, Manuel Gacitúa, Juan A. Fuentes, Dayán Páez‐Hernández, Juan Pablo Peñaloza, Carolina Otero, Marcelo D. Preite, Elı́es Molins, Wesley B. Swords, Gerald J. Meyer, Juan M. Manríquez, Rubén Polanco, I. Chávez, Ramiro Arratia‐Pérez,
Re(CO) 3 + complexes with an ancillary ligand present an electron withdrawing effect suitable for cell imaging.
Tópico(s): Radiopharmaceutical Chemistry and Applications
2016 - Royal Society of Chemistry | New Journal of Chemistry
Juan I. Young, Verónica Otero, Marcelo G. Cerdán, Tomás L. Falzone, Eng-Cheng Chan, Malcolm J. Low, Marcelo Rubinstein,
The pro-opiomelanocortin (POMC) gene is expressed in a subset of hypothalamic and hindbrain neurons and in pituitary melanotrophs and corticotrophs. POMC neurons release the potent opioid beta-endorphin and several active melanocortins that control homeostasis and feeding behavior. POMC gene expression in the CNS is believed to be controlled by distinct cis-acting regulatory sequences. To analyze the transcriptional regulation of POMC in neuronal and endocrine cells, we produced transgenic mice carrying ...
Tópico(s): Cholesterol and Lipid Metabolism
1998 - Society for Neuroscience | Journal of Neuroscience
Produção Nacional
Revisado por pares
Rosalia Méndez‐Otero, Marcelo F. Santiago,
Cell migration occurs extensively during mammalian brain development and persists in a few regions in the adult brain. Defective migratory behavior of neurons is thought to be the underlying cause of several congenital disorders. Knowledge of the dynamics and molecular mechanisms of neuronal movement could expand our understanding of the normal development of the nervous system as well as help decipher the pathogenesis of neurological developmental disorders. In our studies we have identified and ...
Tópico(s): Caveolin-1 and cellular processes
2003 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Produção Nacional
Revisado por pares
Fernanda Gubert, Camila Zaverucha-do-Valle, Pedro M. Pimentel‐Coelho, Rosalia Méndez‐Otero, Marcelo F. Santiago,
During development, radial glia cells contribute to neuronal migration and neurogenesis, and differentiate into astrocytes by the end of the developmental period. Recently, it was demonstrated that during development, radial glia cells, in addition to their role in migration, also give rise to neuroblasts. Furthermore, radial glial cells remain in the adult brain as adult neural stem cells (NSC) in the subventricular zone (SVZ) around the lateral ventricles (LVs), and generate new neurons continuously ...
Tópico(s): Neuroinflammation and Neurodegeneration Mechanisms
2008 - Elsevier BV | Brain Research
Nuno Otero, Marcelo Milrad, Yvonne Rogers, António J. Santos, Manuela Veríssimo, Nuno Torres,
In this paper, we revisit different theoretical and conceptual issues that have been influencing the design of educational technology artefacts. In particular, we take into consideration recent perspectives in cognitive science that acknowledge the important effects of external representations (ERs) in learning and discuss the challenges regarding the applicability of these ideas in connection to seamless-learning environments. Extending the previous work revised here, this paper suggests that in ...
Tópico(s): Visual and Cognitive Learning Processes
2011 - Inderscience Publishers | International Journal of Mobile Learning and Organisation
Jonathas S. Bittencourt, Luciano Artemio Leal, Max C. Langer, Sergio Alex Kugland de Azevedo,
... PVL), Rainer Schoch (SMNS), Ricardo Martínez (PVSJ), Alejandro Otero and Marcelo Reguero (La Plata, Argentina), Diego Pol and Eduardo ...
Tópico(s): Evolution and Paleontology Studies
2012 - Taylor & Francis | Alcheringa An Australasian Journal of Palaeontology
María de los Ángeles Fanaro, María Rita Otero, Marcelo Arlego,
This paper discusses the teaching of basic quantum mechanics in high school. Rather than following the usual formalism, our approach is based on Feynman's path integral method. Our presentation makes use of simulation software and avoids sophisticated mathematical formalism.
Tópico(s): Experimental Learning in Engineering
2012 - American Association of Physics Teachers | The Physics Teacher
Fernando Tourı́s-Otero, Marcelo Cortez-San Martı́n, José Martı́nez-Costas, Javier Benavente,
We have recently shown that the avian reovirus non-structural protein μNS forms cytoplasmic inclusions in transfected cells and recruits σNS to these structures. In the present study we further demonstrate that μNS mediates the association of the major core protein λA, but not of σA or σC, with inclusions, indicating that the recruitment of viral proteins into avian reovirus factories has specificity. Thus, some proteins appear to be initially recruited to factories by association with μNS, whereas ...
Tópico(s): Viral Infections and Immunology Research
2004 - Elsevier BV | Journal of Molecular Biology
Produção Nacional
Revisado por pares
Camila Zaverucha-do-Valle, Louise A. Mesentier‐Louro, Fernanda Gubert, Nicoli Mortari, Ana Beatriz Padilha, Bruno Diaz Paredes, André Luiz Mencalha, Eliana Abdelhay, Camila da Silva Teixeira, Fernanda Guimarães Meireles Ferreira, Fernanda Tovar‐Moll, Sérgio Augusto Lopes de Souza, Bianca Gutfilen, Rosalia Méndez‐Otero, Marcelo F. Santiago,
In adult mammals, the regeneration of the optic nerve is very limited and at the moment there are several groups trying different approaches to increase retinal ganglion cell (RGC) survival and axonal outgrowth. One promising approach is cell therapy. In previous work, we performed intravitreal transplantation of bone-marrow mononuclear cells (BMMCs) after optic nerve crush in adult rats and we demonstrated an increase in RGC survival and axon outgrowth 14 days after injury. In the present work, we ...
Tópico(s): Mesenchymal stem cell research
2014 - Elsevier BV | Brain Research
Produção Nacional
Revisado por pares
Louise A. Mesentier‐Louro, Camila Zaverucha-do-Valle, Almir Jordão da Silva‐Junior, Gabriel Nascimento‐dos‐Santos, Fernanda Gubert, Ana Beatriz Padilha de Figueirêdo, Ana Luiza Torres, Bruno Diaz Paredes, Camila da Silva Teixeira, Fernanda Tovar‐Moll, Rosalia Méndez‐Otero, Marcelo F. Santiago,
Bone marrow-derived cells have been used in different animal models of neurological diseases. We investigated the therapeutic potential of mesenchymal stem cells (MSC) injected into the vitreous body in a model of optic nerve injury. Adult (3–5 months old) Lister Hooded rats underwent unilateral optic nerve crush followed by injection of MSC or the vehicle into the vitreous body. Before they were injected, MSC were labeled with a fluorescent dye or with superparamagnetic iron oxide nanoparticles, ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2014 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Louise A. Mesentier‐Louro, Camila Zaverucha-do-Valle, Paulo Henrique Rosado-de-Castro, Almir Jordão da Silva‐Junior, Pedro M. Pimentel‐Coelho, Rosalia Méndez‐Otero, Marcelo F. Santiago,
Following optic nerve injury associated with acute or progressive diseases, retinal ganglion cells (RGCs) of adult mammals degenerate and undergo apoptosis. These diseases have limited therapeutic options, due to the low inherent capacity of RGCs to regenerate and due to the inhibitory milieu of the central nervous system. Among the numerous treatment approaches investigated to stimulate neuronal survival and axonal extension, cell transplantation emerges as a promising option. This review focuses ...
Tópico(s): Mesenchymal stem cell research
2015 - Hindawi Publishing Corporation | Stem Cells International
Daniel Luna, Daniel A. Rizzato Lede, Carlos Otero, Marcelo Risk, Fernán Gónzalez Bernaldo de Quirós,
Clinical Decision Support Systems can alert health professionals about drug interactions when they prescribe medications. The Hospital Italiano de Buenos Aires in Argentina developed an electronic health record with drug-drug interaction alerts, using traditional software engineering techniques and requirements. Despite enhancing the drug-drug interaction knowledge database, the alert override rate of this system was very high. We redesigned the alert system using user-centered design (UCD) and participatory ...
Tópico(s): Data Quality and Management
2017 - Elsevier BV | Journal of Biomedical Informatics
Produção Nacional
Revisado por pares
Louise A. Mesentier‐Louro, Sara De Nicolò, Pamela Rosso, Luigi Antonio De Vitis, Valerio Castoldi, Letizia Leocani, Rosalia Méndez‐Otero, Marcelo F. Santiago, Paola Tirassa, Paolo Rama, Alessandro Lambìase,
Nerve growth factor (NGF) is suggested to be neuroprotective after nerve injury; however, retinal ganglion cells (RGC) degenerate following optic-nerve crush (ONC), even in the presence of increased levels of endogenous NGF. To further investigate this apparently paradoxical condition, a time-course study was performed to evaluate the effects of unilateral ONC on NGF expression and signaling in the adult retina. Visually evoked potential and immunofluorescence staining were used to assess axonal damage ...
Tópico(s): Axon Guidance and Neuronal Signaling
2017 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Produção Nacional
Revisado por pares
Louise A. Mesentier‐Louro, Pamela Rosso, Valentina Carito, Rosalia Méndez‐Otero, Marcelo F. Santiago, Paolo Rama, Alessandro Lambìase, Paola Tirassa,
Tópico(s): Glaucoma and retinal disorders
2018 - Springer Science+Business Media | Molecular Neurobiology
Produção Nacional
Revisado por pares
Nicanor Obaldía, Elamaran Meibalan, Juliana M. Sá, Siyuan Ma, Martha A. Clark, Pedro Mejia, Roberto R. Moraes Barros, William Otero, Marcelo U. Ferreira, James R. Mitchell, Danny A. Milner, Curtis Huttenhower, Dyann F. Wirth, Manoj T. Duraisingh, Thomas E. Wellems, Matthias Marti,
ABSTRACT Plasmodium vivax causes heavy burdens of disease across malarious regions worldwide. Mature P. vivax asexual and transmissive gametocyte stages occur in the blood circulation, and it is often assumed that accumulation/sequestration in tissues is not an important phase in their development. Here, we present a systematic study of P. vivax stage distributions in infected tissues of nonhuman primate (NHP) malaria models as well as in blood from human infections. In a comparative analysis of ...
Tópico(s): Hepatitis B Virus Studies
2018 - American Society for Microbiology | mBio
José P. O’Gorman, Sergio Santillana, Rodrigo A. Otero, Marcelo Reguero,
Aristonectines show a highly derived morphology among elasmosaurid plesiosaurs, including some species with large body size. A new postcranial skeleton is described from the uppermost Maastrichtian levels of the López de Bertodano Formation, Seymour Island (= Marambio), Antarctica, being referred to as cf. Aristonectes sp; the most striking feature of the specimen described is its large body size, among the largest elasmosaurids worldwide. The occurrence of this specimen, located approximately 2.3 ...
Tópico(s): Ichthyology and Marine Biology
2019 - Elsevier BV | Cretaceous Research
Produção Nacional
Revisado por pares
Louise A. Mesentier‐Louro, Leandro Coelho Teixeira‐Pinheiro, Fernanda Gubert, Juliana Ferreira Vasques, Almir Jordão da Silva‐Junior, Luiza Chimeli-Ormonde, Gabriel Nascimento‐dos‐Santos, Rosalia Méndez‐Otero, Marcelo F. Santiago,
Retina and/or optic nerve injury may cause irreversible blindness, due to degeneration of retinal ganglion cells. We and others have previously shown that the intravitreal injection of mesenchymal stem cells (MSCs) protects injured retinal ganglion cells and stimulates their regeneration after optic nerve injury, but the long-term effects of this therapy are still unknown.We injected rat MSC (rMSC) intravitreally in adult (3-5 months) Lister Hooded rats of either sex after optic nerve crush. Retinal ...
Tópico(s): Anesthesia and Neurotoxicity Research
2019 - BioMed Central | Stem Cell Research & Therapy
Produção Nacional
Revisado por pares
Gabriel Nascimento‐dos‐Santos, Leandro Coelho Teixeira‐Pinheiro, Almir Jordão da Silva‐Junior, Luiza Carvalho, Louise A. Mesentier‐Louro, William W. Hauswirth, Rosalia Méndez‐Otero, Marcelo F. Santiago, Hilda Petrs‐Silva,
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2019 - Springer Nature | Gene Therapy
Johanna Velander, Mohammed Ahmed Taiye, Nuno Otero, Marcelo Milrad,
Abstract Uncovering patterns and trends in vast, ever-increasing quantities of data has been enabled by different machine learning methods and techniques used in Artificial Intelligence (AI) systems. Permeating many aspects of our lives and influencing our choices, development in this field continues to advance and increasingly impacts us as individuals and our society. The risks and unintended effects such as bias from input data or algorithm design have recently stirred discourse about how to inform ...
Tópico(s): Teaching and Learning Programming
2023 - Springer Science+Business Media | Education and Information Technologies
Produção Nacional
Revisado por pares
Luiz Ricardo C. Vasconcellos, Letícia Martimiano, Danillo Pereira Dantas, Filipe Mota Fonseca, Hílton Antônio Mata dos Santos, Leonardo H. Travassos, Rosalia Méndez‐Otero, Marcelo T. Bozza, Pedro M. Pimentel‐Coelho,
Background and Purpose: Heme is a red blood cell component released in the brain parenchyma following intracerebral hemorrhage. However, the study of the pathophysiological mechanisms triggered by heme in the brain is hampered by the lack of well-established in vivo models of intracerebral heme injection. This study aims to optimize and characterize a protocol of intrastriatal heme injection in mice, with a focus on the induction of lipid peroxidation, neuroinflammation and, ultimately, sensorimotor ...
Tópico(s): Neurological and metabolic disorders
2021 - Lippincott Williams & Wilkins | Stroke
Produção Nacional
Revisado por pares
Fernanda Gubert, Igor Bonacossa-Pereira, Ana B. Decotelli, Michelle Furtado, Andréia Vasconcelos-dos-Santos, Rosalia Méndez‐Otero, Marcelo F. Santiago,
Amyotrophic lateral sclerosis (ALS) is a chronic degenerative disease that mainly affects motor neurons, leading to progressive paralysis and death. Recently, cell therapy has emerged as a therapeutic alternative for several neurological diseases, including ALS, and bone-marrow cells are one of the major cell sources. Considering the importance of pre-clinical trials to determine the best therapeutic protocol and the hope of translating this protocol to the clinical setting, we tested bone-marrow ...
Tópico(s): Nerve injury and regeneration
2019 - Elsevier BV | Brain Research
José P. O’Gorman, Rodrigo A. Otero, Marcelo Reguero, Zulma Gasparini,
The last twenty million years (Maastrichtian–Santonian) of Southern Hemisphere plesiosaur history is especially well recorded in the Weddellian Province (Patagonia; Western Antarctica and New Zealand). The oldest Late Cretaceous plesiosaurs, two specimens referred to Polycotylidae indet., come from the Santonian levels of the Santa Marta Formation, while the oldest elasmosaurids come from the lower Campanian of the same formation. In the lower Maastrichtian of the Snow Hill Island Formation the non- ...
Tópico(s): Ichthyology and Marine Biology
2019 - Science Press | ADVANCES IN POLAR SCIENCE
Colleen Hamilton, John Whalley,
... Ikiara (Kenya), Nguyuru H.I. Lipumba (Tanzania), Manuel Otero (Argentina), T. Ademola Oyejide (Nigeria), Marcelo de Paiva Abreu (Brazil), and Eduardo Perez Motta ( ... Ikiara (Kenya), Nguyuru H.I. Lipumba (Tanzania), Manuel Otero (Argentina), T. Ademola Oyejide (Nigeria), Marcelo de Paiva Abreu (Brazil), and Eduardo Perez Motta ( ... Ikiara (Kenya), Nguyuru H.I. Lipumba (Tanzania), Manuel Otero (Argentina), T. Ademola Oyejide (Nigeria), Marcelo de Paiva Abreu (Brazil), and Eduardo Perez Motta ( ... Ikiara (Kenya), Nguyuru H.I. Lipumba (Tanzania), Manuel Otero (Argentina), T. Ademola Oyejide (Nigeria), Marcelo de Paiva Abreu (Brazil), and Eduardo Perez Motta ( ...
Tópico(s): Global Financial Crisis and Policies
1995 - Wiley | World Economy
Produção Nacional
Revisado por pares
Rosalia Méndez‐Otero, Marcelo F. Santiago,
Migration of neurons from their site of origin to their final destination is a critical and universal step in the formation of the complex structure of the nervous system. The migratory process is thought to be governed in part by genetically and epigenetically defined sequences of signals which are interpreted by migrating cells. The molecular mechanisms that underlie neuronal migration have been the subject of intense investigation. As in other developmental processes, many molecules must participate ...
Tópico(s): RNA Interference and Gene Delivery
2001 - Brazilian Academy of Sciences | Anais da Academia Brasileira de Ciências
Marcelo Otero, Hernán G. Solari,
We present a stochastic dynamical model for the transmission of dengue that takes into account seasonal and spatial dynamics of the vector Aedes aegypti. It describes disease dynamics triggered by the arrival of infected people in a city. We show that the probability of an epidemic outbreak depends on seasonal variation in temperature and on the availability of breeding sites. We also show that the arrival date of an infected human in a susceptible population dramatically affects the distribution ...
Tópico(s): Mathematical and Theoretical Epidemiology and Ecology Models
2009 - Elsevier BV | Mathematical Biosciences
Ernesto J. Calvo, Erica Forzani, Marcelo Otero,
We describe quartz crystal electroacoustic admittance studies in thickness shear mode resonators loaded with self-assembled multilayers composed of alternate layers of glucose oxidase (GOx) and poly(allylamine) covalently attached to [Os(bpy)2ClPyCOH]-, (PAH−Os), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold on the quartz crystal. The complex acoustic impedance parameters, RS and XLS of a lumped-element Butterworth−Van Dyke (BVD) resonator have been determined for organized thin films ...
Tópico(s): Force Microscopy Techniques and Applications
2002 - American Chemical Society | Analytical Chemistry
Erica Forzani, Marcelo Otero, Manuel A. Pérez, Manuel López Teijelo, Ernesto J. Calvo,
Self-assembled multilayers composed of alternated layers of glucose oxidase (GOx) and poly(allylamine) covalently attached to [Os(bpy)2ClPyCOH]+ (PAH−Os), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold surface were studied "ex situ" and "in situ" (with the films in contact with water) by ellipsometry and quartz crystal microbalance (QCM). The ellipsometric parameters of the thiol film on gold in the first layer were analyzed in terms of an anisotropic single-layer model. For the subsequent ( ...
Tópico(s): Surfactants and Colloidal Systems
2002 - American Chemical Society | Langmuir
Ernesto J. Calvo, Erica Forzani, Marcelo Otero,
The ellipsometric and mass increase during the oxidation–reduction cycles of self-assembled layer-by-layer osmium complex derivatized poly(allylamine) and glucose oxidase multilayers due to the exchange of anions and solvent with the electrolyte and viscoelastic changes of the surface layer have been studied using the electrochemical quartz crystal microbalance (EQCM) technique. From the measurement of the quartz crystal impedance spectrum of films deposited on a thiolate Au-quartz crystal immersed ...
Tópico(s): Polymer Surface Interaction Studies
2002 - Elsevier BV | Journal of Electroanalytical Chemistry