Marie Brázdová, Vlastimil Tichý, Robert Helma, Pavla Bažantová, Alena Polášková, Aneta Krejčí, Marek Petr, Lucie Navrátilová, Olga Tichá, Karel Nejedlý, Martin L. Bennink, Vinod Subramaniam, Zuzana Bábková, Tomáš Martínek, Matej Lexa, Matej Adámik,
Triplex DNA is implicated in a wide range of biological activities, including regulation of gene expression and genomic instability leading to cancer. The tumor suppressor p53 is a central regulator of cell fate in response to different type of insults. Sequence and structure specific modes of DNA recognition are core attributes of the p53 protein. The focus of this work is the structure-specific binding of p53 to DNA containing triplex-forming sequences in vitro and in cells and the effect on p53- ...
Tópico(s): RNA modifications and cancer
2016 - Public Library of Science | PLoS ONE
Petr Mohyla, Jiří Hajnyš, Kristýna STERNADELOVÁ, Lucie Krejčí, Marek Pagáč, Kateřina Konečná, Pavel Krpec,
This work is focused on the analysis of the influence of welding on the properties and microstructure of the AISI316L stainless steel tube produced by 3D printing, specifically the SLM (Selective Laser Melting) method. Both non-destructive and destructive tests, including metallographic and fractographic analyses, were performed within the experiment. Microstructure analysis shows that the initial texture of the 3D print disappears toward the fuse boundary. It is evident that high temperature during ...
Tópico(s): Welding Techniques and Residual Stresses
2020 - Multidisciplinary Digital Publishing Institute | Materials
Jolanta Polkowska, Petr Krejčí, Marek Snochowski,
Three-month old Polish Lowland female lambs were fed isocaloric diets containing 14.2% (standard) or 8.1% of proteins for twenty weeks. Changes in body weight were characteristic for normal growth performance in all animals, but daily body gain calculated for the whole experimental period was significantly lower in lambs fed a low protein diet (87.9 +/- 13.5 and 158 +/- 13.8 g/day, respectively). Two series of blood collections (4 hrs with 15 min interval) were performed at age of 21 and 26 weeks in order ...
Tópico(s): Regulation of Appetite and Obesity
2009 - Thieme Medical Publishers (Germany) | Experimental and Clinical Endocrinology & Diabetes
Jakub Krejčí, Marek Babiuch, Ján Babjak, Jiří Suder, Rostislav Wierbica,
The article describes the use of embedded systems in the Industrial Internet of Things and its benefits for industrial robots. For this purpose, the article presents a case study, which deals with an embedded system using an advanced microcontroller designed to be placed directly on the robot. The proposed system is being used to collect information about industrial robot parameters that impact its behavior and its long-term condition. The device measures the robot's surroundings parameters and ...
Tópico(s): Modular Robots and Swarm Intelligence
2022 - Multidisciplinary Digital Publishing Institute | Micromachines
Ondřej Horký, Jiřı́ Mayer, Lenka Kablásková, Filip Rázga, Marta Krejčí, Jarmila Kissová, Marek Borský, Ivana Ježíšková, D. Dvořáková,
Summary Introduction: The reoccurrence or increase in autologous hematopoiesis after allogeneic transplantation has been linked to incipient leukemia relapse. However, the importance of such an emergency regarding microchimerism (i.e. mixed chimerism below 1% of autologous cells) still remains controversial, as fluctuating microchimerism can be observed for a very long time after transplantation. Methods: Using real‐time PCR (RQ‐PCR), we compare peripheral blood samples obtained from patients with ...
Tópico(s): Acute Myeloid Leukemia Research
2010 - Wiley | International Journal of Laboratory Hematology
Tomáš Krejčí, Ivo Dostál, Marek Havlíček, Stanislav Martinát,
Tópico(s): Migration, Aging, and Tourism Studies
2016 - Elsevier BV | Transportation Research Part D Transport and Environment
Jaroslav Meluzı́n, Lenka Špinarová, P Hude, Jan Krejčí, Helena Podroužková, Martin Pešl, Marek Orban, Ladislav Dušek, Jiří Jarkovský, Josef Kořínek,
The ratio of early diastolic transmitral flow velocity (E) to early diastolic mitral annular velocity (Ea) is frequently used to predict an increase in left ventricular filling pressure (LVFP). However, this approach has several limitations. The aim of this study was to test whether additional information is gained by new echocardiographic indexes utilizing strain and strain rate (SR) derived from 2-dimensional speckle tracking echocardiography (2D-STE) for the estimation of LVFP. Fifty-one patients ...
Tópico(s): Cardiac Imaging and Diagnostics
2010 - Oxford University Press | European Journal of Echocardiography
Petr Klusáček, Filip Alexandrescu, Robert Osman, Jiří Malý, Josef Kunc, Petr Dvořák, Bohumil Frantál, Marek Havlíček, Tomáš Krejčí, Stanislav Martinát, Hana Skokanová, Jakub Trojan,
The application of principles of good governance in brownfield regeneration, for instance through improved transparency and participation of various groups of stakeholders, varies between regions and cities. In this article, we approach good governance as a strategic response of actors in the struggle for creating development opportunities on brownfield land. Good governance has been mostly seen as a normative consideration, but it is not clear why regions with lower development prospects would ...
Tópico(s): Urban Planning and Governance
2018 - Elsevier BV | Land Use Policy
Firoozeh Sahebi, Simona Iacobelli, Giulia Sbianchi, Linda Köster, Didier Blaise, Péter Reményi, Nigel H. Russell, Per Ljungman, Guido Kobbe, Jane F. Apperley, Marek Trněný, Marta Krejčí, W Wiktor-Jędrzejczak, James F. Sanchez, Nicolaas Schaap, Cecilia Isaksson, Stig Lenhoff, Paul Browne, Christof Scheid, Keith Wilson, Ibrahim Yakoub‐Agha, Soledad González Muñiz, Stefan Schönland, Curly Morris, Laurent Garderet, Nicolaus Kröger,
The advent of novel agents for multiple myeloma (MM) is cause for a re-examination of the incidence of second primary malignancies (SPMs). We examined the SPM rate in MM patients who were enrolled in the prospective observational CALM (Collaboration to Collect Autologous Transplant outcome in Lymphoma and Myeloma) study. Between 2008 and 2012, 3204 patients with MM underwent a first autologous hematopoietic stem cell transplantation. Plerixafor was used as a mobilizing agent for patients with poor ( ...
Tópico(s): Lung Cancer Research Studies
2018 - Elsevier BV | Biology of Blood and Marrow Transplantation
Alexandra Sisáková, Veronika Altmannová, Marek Šebesta, Lumír Krejčí,
Proper DNA replication is essential for faithful transmission of the genome. However, replication stress has serious impact on the integrity of the cell, leading to stalling or collapse of replication forks, and has been determined as a driving force of carcinogenesis. Mus81-Mms4 complex is a structure-specific endonuclease previously shown to be involved in processing of aberrant replication intermediates and promotes POLD3-dependent DNA synthesis via break-induced replication. However, how replication ...
Tópico(s): Cancer-related Molecular Pathways
2017 - BioMed Central | BMC Biology
Jaroslav Meluzı́n, Lenka Špinarová, Petr Hude, Jan Krejčí, Hana Poloczková, Helena Podroužková, Martin Pešl, Marek Orban, Ladislav Dušek, Josef Kořínek,
Background In idiopathic dilated cardiomyopathy (IDC), myocardial deformational parameters and their mutual relationships remain incompletely characterized. Methods Thirty-seven patients with IDC underwent two-dimensional speckle-tracking echocardiography (2D-STE) to assess left ventricular rotation, torsion, and longitudinal, circumferential, and radial systolic and diastolic strains and strain rates. Additionally, 2D-STE was performed in 14 controls. Results All deformational parameters on 2D-STE ...
Tópico(s): Cardiac Imaging and Diagnostics
2009 - Elsevier BV | Journal of the American Society of Echocardiography
Ondřej Fiala, Miloš Pešek, Jindřich Fínek, Jana Krejčí, Jan Říčař, Zdeněk Bortlíček, Lucie Benešová, Marek Minárik,
Erlotinib is an epidermal growth factor receptor tyrosine kinase inhibitor used in treatment of advanced NSCLC.Patients harboring EGFR or KRAS mutations represent minority of all patients in caucasian population and there is no available predictor for a predominant group of patients harboring the wild-type EGFR and wild-type KRAS genes.Skin rash is the most frequent manifestation of cutaneous toxicity of erlotinib.Rash is associated with a good therapeutic response.We aimed at the evaluation of ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2012 - AEPress | Neoplasma
Jiří Minařík, Petr Pavlíček, Luděk Pour, Tomáš Pika, Vladimír Maisnar, Ivan Špıčka, Jiří Jarkovský, Marta Krejčí, Jaroslav Bačovský, Jakub Radocha, Jan Štraub, Petr Kessler, Marek Wróbel, Lenka Walterová, M. Sýkora, Jarmila Obernauerová, Lucie Brožová, Evžen Gregora, D. Adamová, Jaromír Gumulec, Zdeněk Adam, Vlastimil Ščudla, Roman Hájek,
Objective Subcutaneous (SC) application of bortezomib has been recently introduced as a new application route in multiple myeloma (MM) patients. We performed an analysis to compare the outcomes of bortezomib-based therapy in multiple myeloma (MM) patients treated using either intravenous (IV) or subcutaneous (SC) route of administration. Patients and methods During January 2012 through December 2013, we performed a retrospective analysis of 446 patients with MM treated with bortezomib-based regimens ( ...
Tópico(s): Protein Degradation and Inhibitors
2015 - Public Library of Science | PLoS ONE
Jiří Blažek, Petr Hošek, Karolína Hrabcová, Monika Bratová, Juraj Kultan, Michal Hrnčiarik, Markéta Černovská, Petra Zemanová, Jana Krejčí, Leona Koubková, Marek Šťastný, Martin Svatoň,
The aim of this study was to investigate possible association between adverse events of nivolumab therapy and the effectiveness of treatment in patients with non-small cell lung cancer (NSCLC). Focusing on serious adverse events (i.e., those of grade ≥3), we evaluated overall survival (OS), progression-free survival (PFS), as well as objective response rate (ORR) to treatment.We retrospectively analyzed a set of patients from the TULUNG database of NSCLC treated with nivolumab in eight oncology centers. ...
Tópico(s): Colorectal Cancer Treatments and Studies
2023 - Stanford University Highwire Press | In Vivo
Martin Maděrka, Vladimír Dvořák, Jozef Hambálek, David Stejskal, G Krejčí, Marek Švesták, Kateřina Langová, R Pilka,
Objective The aim of this study was to compare TFF3, AIF-1, S100-A11 and DKK1 serum levels in patients with cervical dysplasia, and in healthy female controls. Methods The first group included 59 patients with a histological dia-gnosis of precancerous disease CIN 1. The second group included 198 patients with a histological dia-gnosis of precancerous disease CIN 2 or CIN 3. The control group was comprised of 90 patients who underwent elective total hysterectomy for nonmalignant disorders. In all ...
Tópico(s): Cervical Cancer and HPV Research
2021 - Czech Medical Association of J. E. Purkyně | Česká gynekologie
Linda M. Janelli, Genevieve W. Kanski, Mary Neary,
... 49-62), Online publication date: 1-Mar-1997. Marek K, Rantz M, Fagin C and Krejci J (2021) OBRA '87: Has It Resulted in ...
Tópico(s): Elder Abuse and Neglect
1994 - Slack Incorporated (United States) | Journal of Gerontological Nursing
Olga Stehlíková, Jana Chovancová, Boris Tichý, Marta Krejčí, Yvona Brychtová, Anna Panovská, H. Francová Skuhrová, Kateřina Burčková, Marek Borský, Tomáš Loja, Jiřı́ Mayer, Šárka Pospı́šilová, Michael Doubek,
Summary Introduction Minimal residual disease ( MRD ) detection has become increasingly important for the assessment of therapy response in chronic lymphocytic leukemia ( CLL ). However, current MRD analysis methods, both molecular genetic and flow cytometric, are time‐consuming and require experienced laboratory staff. Methods To reduce the demands of flow cytometric MRD detection in CLL , we have introduced a novel flow cytometric 8‐color protocol. The MRD analysis results using this protocol were then compared ...
Tópico(s): Lymphoma Diagnosis and Treatment
2013 - Wiley | International Journal of Laboratory Hematology
Ondřej Fiala, Miloš Pešek, Jindřich Fínek, Jana Krejčí, Zdeněk Bortlíček, Lucie Benešová, Marek Minárik,
Molecular targeted therapy based on tyrosine kinase inhibitors, directed at the epidermal growth factor receptor (EGFR) is one of novel options for management of NSCLC.Erlotinib is EGFR tyrosine kinase inhibitor used for treatment of the advanced NSCLC.This presented study is focused on comparison of erlotinib and chemotherapy efficacy in the second line treatment of the advanced NSCLC.DCR and PFS became the primary endpoints.Total number of patients was 290.A group treated with chemotherapy in the ...
Tópico(s): Colorectal Cancer Treatments and Studies
2012 - AEPress | Neoplasma
Natália Káňavová, Anna Krejčí, Martina Benedeková, Marek Doležel, Lubomír Machuča,
Tópico(s): Renal function and acid-base balance
2015 - Springer Science+Business Media | Chemical Papers
Veronika Válková, Jitka Jirčíková, M Trnková, Kateřina Steinerová, Petra Keslová, M. Lánská, Zdeněk Kořı́stek, Luděk Raida, Marta Krejčí, K. Kruntoradova, Tomáš Doležal, Kateřina Benešová, Petr Cetkovský, Marek Trněný,
Although allogeneic haematopoietic stem cell transplantation (allo-HSCT) offers a unique curative potential, it may be connected with high treatment-related morbidity and mortality.Besides many organ complications, allo-HSCT may significantly affect quality of life (QOL).Patients and methods: Between January 2011 and December 2012, five hundred and ninety patients (pts) from 6 transplant centers in the Czech Republic filled in the questionnaire for the quantitative measurement of QOL using Functional ...
Tópico(s): Acute Lymphoblastic Leukemia research
2016 - AEPress | Neoplasma
Marek Šebesta, Peter Burkovics, Lajos Haracska, Lumír Krejčí,
The error-free repair of double-strand DNA breaks by homologous recombination (HR) ensures genomic stability using undamaged homologous sequence to copy genetic information. While some of the aspects of the initial steps of HR are understood, the molecular mechanisms underlying events downstream of the D-loop formation remain unclear. Therefore, we have reconstituted D-loop-based in vitro recombination-associated DNA repair synthesis assay and tested the efficacy of polymerases Pol δ and Pol η to ...
Tópico(s): PARP inhibition in cancer therapy
2011 - Elsevier BV | DNA repair
Peter Burkovics, Marek Šebesta, D. Balogh, Lajos Haracska, Lumír Krejčí,
Stalling of replication forks at unrepaired DNA lesions can result in discontinuities opposite the damage in the newly synthesized DNA strand. Translesion synthesis or facilitating the copy from the newly synthesized strand of the sister duplex by template switching can overcome such discontinuities. During template switch, a new primer–template junction has to be formed and two mechanisms, including replication fork reversal and D-loop formation have been suggested. Genetic evidence indicates a ...
Tópico(s): Polyomavirus and related diseases
2013 - Oxford University Press | Nucleic Acids Research
Marek Petr, Robert Helma, Alena Polášková, Aneta Krejčí, Zuzana Dvořáková, Iva Kejnovská, Lucie Navrátilová, Matej Adámik, Michaela Vorlı́čková, Marie Brázdová,
G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. ...
Tópico(s): Bacteriophages and microbial interactions
2016 - Portland Press | Bioscience Reports
Marek Špinka, Gudrun Illmann, Zuzana Štětková, Petr Krejčí, M. Tománek, Lech Sedlak, J Lidický,
It has been established that sows up- or down-regulate their milk production as the frequency of nursings is changed. The amount of udder massage by piglets might also influence milk production. To investigate whether these effects are associated with changes in prolactin or insulin levels, we enforced five sows each to nurse either every 35 min (MIN35) or every 70 min (MIN70) over a 26- to 28-hr period. Milk production was measured during the first 24 hr of this period. During the last three to four ...
Tópico(s): Meat and Animal Product Quality
1999 - Elsevier BV | Domestic Animal Endocrinology
Vojtěch Petr, Petra Hrubá, Marek Kollár, Karel Krejčí, Roman Šafránek, Soňa Štěpánková, Jarmila Dědochová, Jana Machová, Jakub Zieg, Janka Slatinská, Eva Pokorná, Ondřej Viklický,
Background. Thrombotic microangiopathy (TMA) significantly affects kidney graft survival, but its pathophysiology remains poorly understood. Methods. In this multicenter, retrospective, case–control paired study designed to control for donor-associated risks, we assessed the recipients’ risk factors for de novo TMA development and its effects on graft survival. The study group consists of patients with TMA found in case biopsies from 2000 to 2019 (n = 93), and the control group consists of recipients ...
Tópico(s): Organ Donation and Transplantation
2021 - Wolters Kluwer | Transplantation Direct
Helena Bedáňová, Jiří Ondrášek, Jan Černý, Marek Orban, Lenka Špinarová, Petr Hude, Jan Krejčí, Petr Němec,
Heart transplantation (HTx) is the most effective therapy in patients with end-stage heart failure. Diabetes-related complications are relative contraindications for heart transplantation. The aim of our study was to analyse the impact of DM (diabetes mellitus) at the time of heart transplantation and new-onset post-transplantation DM on long-term survival.A retrospective database analysis was performed on all patients who had undergone HTx at our institution between 4/1997 and 9/2007. Patients were ...
Tópico(s): Organ Transplantation Techniques and Outcomes
2009 - Palacký University Olomouc | Biomedical Papers
Jakub Krejčí, Marek Babiuch, Jiří Suder, Václav Krys, Zdenko Bobovský,
Tópico(s): Blockchain Technology Applications and Security
2025 - Multidisciplinary Digital Publishing Institute | Sensors
Jan Čadil, Kristýna Vltavská, Igor Krejčí, David Hartman, Marek Brabec,
Aggregate production function, especially in its Cobb-Douglas and more general CES form, is still very popular among economists.However there is a huge and long-standing critique regarding the function.The core of the criticism which prevailed until these days is that not only the Cobb-Douglas but also CES aggregate production functions are nothing more than income identity in disguise.Such fact would have serious implications for contemporary macroeconomics -both theory and practice.In this paper ...
Tópico(s): Economic Theory and Institutions
2017 - International Institute of Social and Economic Sciences | International Journal of Economic Sciences
Ivan Literák, Lavrentis Sidiropoulos, Jan Škrábal, Thijs Valkenburg, Šimon Krejčí, Marek Dostál, Elisabeth Navarrete, Dimitris P. Vasilakis,
The main reasons for the global biodiversity decline are the destruction and degradation of natural habitats caused by human activity by transforming them into agricultural lands. Although this transformation has been linked with decreased biodiversity, some bird species might have learned how to benefit from newly created habitats. We studied home ranges and habitat preferences for daytime activity and roosting of two sedentary Marsh Harriers (Circus aeruginosus) from the Evros Delta and the Mediterranean ...
Tópico(s): Marine and coastal plant biology
2023 - The Waterbird Society | Waterbirds
Martin Maděrka, Vladimír Dvořák, Jozef Hambálek, David Stejskal, G Krejčí, Marek Švesták, Kateřina Langová, R Pilka,
Objective The aim of this study was to compare the serum levels of TFF3, AIF-1, S100-A11 and DKK1 in surgically staged patients with cervical cancer, and in healthy female controls. Methods In total 85 consecutive patients dia-gnosed at the Department of Obstetrics and Gynecology, University Hospital in Olomouc with cervical cancer undergoing radical hysterectomy or fertility sparing surgery with pelvic lymphadenectomy were included. Ninety patients who underwent elective total hysterectomy for ...
Tópico(s): Endometrial and Cervical Cancer Treatments
2021 - Czech Medical Association of J. E. Purkyně | Česká gynekologie