Martin Procházka, Jana Procházková, Marek Ľubušký, Radovan Pilka, Jana Úlehlová, Igor Michalec, Petr Polák, Marian Kacerovský, Luděk Slavík,
The study aimed at finding a laboratory approach to detect endothelial damage in normal pregnancy as well as in pregnancy complicated by preeclampsia using selected markers of endothelial activation.A total of 403 healthy pregnant women without a history of deep vein thrombosis and/or hypertension were prospectively studied. From all women, venous blood was collected before the end of the 1st trimester, between weeks 24 and 28 of gestation, and in the 3rd trimester (weeks 34-36). Assays of tissue ...
Tópico(s): Biomarkers in Disease Mechanisms
2015 - Clinical Laboratory Publications | Clinical Laboratory
Ivana Musilová, Ctirad Andrýs, Magdaléna Holečková, Veronika Kolárová, Lenka Plíšková, Marcela Drahošová, Radka Bolehovská, Radovan Pilka, K Huml, Teresa Cobo, Bo Jacobsson, Marian Kacerovský,
Introduction: We aimed to compare the amniotic fluid interleukin (IL)-6 concentrations measured using the automated electrochemiluminescence immunoassay method and ELISA, and to establish an IL-6 concentration cut-off value for intra-amniotic inflammation (IAI) in preterm prelabor rupture of membranes (PPROM), which can be used in the automated electrochemiluminescence immunoassay method.Materials and methods: A total of 120 women with PPROM were included in this study. Amniotic fluid samples were ...
Tópico(s): Congenital Anomalies and Fetal Surgery
2018 - Informa | The Journal of Maternal-Fetal & Neonatal Medicine
Tomáš Oždian, Jan Vodička, Jiří Dostál, Dušan Holub, Jana Václavková, Michal Ješeta, Barbora Hamerníková, Pavla Kouřilová, Ondřej Malchar, Vladimír Dvořák, Pavel Hejtmánek, Kateřina Sobková, Pavel Ventruba, Radovan Pilka, Petr Džubák, Marián Hajdúch,
Cervical mucus (CM) is a viscous fluid that is produced by the cervical glands and functions as a uterine cervix plug. Its viscosity decreases during ovulation, providing a window for non-invasive sampling. This study focuses on proteomic characterization of CM to evaluate its potential as a non-invasively acquired source of biomarkers and in understanding of molecular (patho)physiology of the female genital tract. The first objective of this work was to optimize experimental workflow for CM processing ...
Tópico(s): Reproductive Physiology in Livestock
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Markéta Pospíšková, Michaela Špenerová, R Pilka, M Kudela, Marián Hajdúch, V Srámek, Bohuslav Melichar, Karel Cwiertka,
Epithelial ovarian carcinoma (EOC) is a highly chemosensitive tumor, but most patients with advanced EOC initially responding to first-line chemotherapy will eventually relapse. Chemosensitivity testing may offer an opportunity for the optimal selection of chemotherapeutic agents for individual patients. In the present retrospective analysis we have examined the changes in chemosensitivity profiles during the course of the disease. Chemosensitivity was determined using the 3-(4,5-dimethylthiazol- ...
Tópico(s): Ovarian cancer diagnosis and treatment
2010 - IMR Press | European Journal of Gynaecological Oncology
Jan Vodička, Jiřı́ Dostál, Dušan Holub, Radovan Pilka, Petr Džubák, Marián Hajdúch, Tomáš Oždian,
Cervical mucus is a viscous fluid functioning as a cervix plug. Products of the endometrial and cervical glands can be detected in the cervical mucus. Cervical mucus is further enriched with transudate originating from the fallopian tubes and proteins originating from the ovaries, peritoneum and distant tissues. With increasing levels of ovarian estrogens, the properties of cervical mucus for possible collection and processing change appropriately. For these reasons, we chose a group of 10 patients ...
Tópico(s): Sperm and Testicular Function
2023 - Czech Medical Association of J. E. Purkyně | Česká gynekologie