Piotr Laudański, Gabriela Rogalska, Damian Warzecha, Michał Lipa, Grzegorz Mańka, Mariusz Kiecka, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Toomas Neuman, Priit Adler, Hedi Peterson, Andres Salumets, Mirosław Wielgoś,
Abstract STUDY QUESTION Are there specific autoantibody profiles in patients with endometriosis that are different from those in controls? SUMMARY ANSWER This study did not reveal a significantly higher prevalence of autoantibodies in the studied groups of patients. WHAT IS KNOWN ALREADY Various inflammatory factors are postulated to be involved in the pathomechanisms of endometriosis, and a potential link exists with autoimmune diseases, which may also play an important role. As the diagnosis of ...
Tópico(s): Endometrial and Cervical Cancer Treatments
2023 - Oxford University Press | Human Reproduction
Damian Warzecha, Julia Załęcka, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Mirosław Wielgoś, Łukasz Ołdak, Anna Leśniewska, Ewa Gorodkiewicz, Piotr Laudański,
Laparoscopy as a diagnostic tool for patients with suspected endometriosis is associated with several potentially life-threatening complications. Therefore, it is imperative to identify reliable, non-invasive biomarkers of the disease. The aim of this study was to analyse the concentrations of fibronectin and type IV collagen in peritoneal fluid and plasma to assess their role as potential biomarkers in the diagnosis of endometriosis. Fibronectin and collagen IV protein levels were assessed by surface ...
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Ksawery Goławski, Robert Soczewica, Joanna Kacperczyk‐Bartnik, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Damian Warzecha, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Mirosław Wielgoś, Ewa Koc‐Żórawska, Marcin Żórawski, Piotr Laudański,
Cadherin 12 (CDH 12) can play a role in the pathogenesis of endometriosis. The aim of this study was to compare the levels of cadherin 12 in the peritoneal fluid between women with and without endometriosis. This was a multicenter cross-sectional study. Eighty-two patients undergoing laparoscopic procedures were enrolled in the study. Cadherin 12 concentrations were determined using the enzyme-linked immunosorbent assay. The level of statistical significance was set at p < 0.05. No differences in cadherin ...
Tópico(s): Gynecological conditions and treatments
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Monika Wróbel, Zielińska Zuzanna, Łukasz Ołdak, Aleksandra Kalicka, Grzegorz Mańka, Mariusz Kiecka, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Piotr Pierzyński, Cezary Wojtyła, Michał Lipa, Damian Warzecha, Mirosław Wielgoś, Włodzimierz Sawicki, Ewa Gorodkiewicz, Piotr Laudański,
Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study—plasma was collected ...
Tópico(s): Reproductive System and Pregnancy
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Barbara Lisowska‐Myjak, Ewa Skarżyńska, Monika Wróbel, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Damian Warzecha, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Mirosław Wielgoś, Piotr Laudański,
An evaluation of the association between the concentrations of vitamin D-binding protein and lactoferrin in the plasma and peritoneal fluid may facilitate the elucidation of molecular mechanisms in endometriosis. Vitamin D-binding protein and lactoferrin concentrations were measured by ELISA in plasma and peritoneal fluid samples from 95 women with suspected endometriosis as classified by laparoscopy into groups with (n = 59) and without endometriosis (n = 36). There were no differences (p > 0.05) in the ...
Tópico(s): Intestinal and Peritoneal Adhesions
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Ewa Skarżyńska, Monika Wróbel, Hanna Zborowska, Mateusz Franciszek Kołek, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Damian Warzecha, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Mirosław Wielgoś, Barbara Lisowska‐Myjak, Piotr Laudański,
The aim of this study was to investigate the relationship between lactoferrin and iron and its binding proteins in women with endometriosis by simultaneously measuring these parameters in plasma and peritoneal fluid. Ninety women were evaluated, of whom 57 were confirmed as having endometriosis. Lactoferrin was measured by ELISA, transferrin, ferritin and iron on a Cobas 8000 analyser. Lactoferrin and transferrin in peritoneal fluid were lower compared to plasma, in contrast to ferritin and iron. ...
Tópico(s): Intestinal and Peritoneal Adhesions
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Paweł Bartnik, Joanna Kacperczyk‐Bartnik, Ksawery Goławski, Janusz Sierdziński, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Damian Warzecha, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Krzysztof Czajkowski, Mirosław Wielgoś, Ewa Koc‐Żórawska, Marcin Żórawski, Piotr Laudański,
Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial−mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical ...
Tópico(s): Intestinal and Peritoneal Adhesions
2022 - Multidisciplinary Digital Publishing Institute | Biomedicines
Julia Załęcka, Zuzanna Zielińska, Łukasz Ołdak, Agata Sakowicz, Grzegorz Mańka, Mariusz Kiecka, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Piotr Pierzyński, Michał Ciebiera, Cezary Wojtyła, Michał Lipa, Damian Warzecha, Mirosław Wielgoś, Krzysztof Cendrowski, Ewa Gorodkiewicz, Piotr Laudański,
Endometriosis is a common disease with a complex pathomechanism and atypical symptoms, often leading to delayed diagnosis. Currently, the sole method for confirming the presence of the disease is through laparoscopy and histopathological examination of collected tissue. However, this invasive procedure carries potential risk and complications, necessitating the exploration of non-surgical diagnostic methods for endometriosis. This study aims to analyze peritoneal fluid and plasma samples for the ...
Tópico(s): Vitamin D Research Studies
2024 - Elsevier BV | Advances in Medical Sciences
Aleksandra Zyguła, Anna Sankiewicz, Agata Sakowicz, Ewa Dobrzyńska, Agnieszka Dakowicz, Grzegorz Mańka, Mariusz Kiecka, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Piotr Pierzyński, Cezary Wojtyła, Michał Lipa, Damian Warzecha, Mirosław Wielgoś, Krzysztof Cendrowski, Ewa Gorodkiewicz, Piotr Laudański,
Background The aim of this study was to analyze the concentration of leptin in peritoneal fluid and plasma and to assess their role as potential biomarkers in the diagnosis of endometriosis. Materials & methods Leptin adjusted for BMI (leptin/BMI ratio) was measured using surface plasmon resonance imaging (SPRI) biosensors. Patients with suspected endometriosis were included in the study. Plasma was collected from 70 cases, and peritoneal fluid from 67 cases. Based on the presence of endometriosis ...
Tópico(s): Endometrial and Cervical Cancer Treatments
2024 - Frontiers Media | Frontiers in Endocrinology
Małgorzata Rogalińska, Jerzy Z. Błoński, Paweł Góralski, Ewa Wawrzyniak, Mariusz L. Hartman, Aneta Rogalska, Paweł Robak, Aneta Koceva‐Chyła, Henryk Piekarski, Tadeusz Robak, Zofia M. Kiliańska,
To improve the efficacy of therapeutic options in chronic lymphocytic leukemia (CLL) an in vitro system to determine the response of mononuclear blood cells from blood of patients was elaborated. The study combines four approaches, i.e., cell viability, apoptosis rate, differential scanning calorimetry (DSC), and immunoblotting to develop personalized therapy protocols based on the cell sensitivity to drug exposure of individual CLL patients. The complementary analyses were performed on 28 peripheral ...
Tópico(s): Acute Lymphoblastic Leukemia research
2015 - Spandidos Publishing | International Journal of Oncology
Joanna Kacperczyk‐Bartnik, Paweł Bartnik, Ksawery Goławski, Janusz Sierdziński, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Damian Warzecha, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Krzysztof Czajkowski, Mirosław Wielgoś, Ewa Koc‐Żórawska, Marcin Żórawski, Piotr Laudański,
The evidence of poly (ADP-ribose) polymerase (PARP) association with the immune response could be coherent with the immunological theory of endometriosis and suggests the possibility of a new research direction. The aim of the study was to evaluate the levels of PARP in plasma and peritoneal fluid of patients with and without endometriosis. It was a multicenter, cross-sectional study. Plasma and peritoneal fluid samples were collected from patients with and without endometriosis during planned laparoscopic ...
Tópico(s): Ovarian cancer diagnosis and treatment
2022 - Multidisciplinary Digital Publishing Institute | Biomedicines
Monika Wróbel, Barbara Lisowska‐Myjak, Ewa Skarżyńska, Grzegorz Mańka, Mariusz Kiecka, Michał Lipa, Damian Warzecha, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Mirosław Wielgoś, Piotr Laudański,
Aim: Biochemical parameters and their interrelationships in plasma and peritoneal fluid (PF) may facilitate understanding of biological processes associated with endometriosis. Selection of vitamin D binding protein (VDBP) and lactoferrin (LF) is based on their characteristic properties and association with inflammatory processes. The aim of this study was to assess the concentrations and correlations of VDBP and LF in plasma and PF in order to investigate systemic and local pathology associated with ...
Tópico(s): Endometriosis Research and Treatment
2024 - Elsevier BV | European Journal of Obstetrics & Gynecology and Reproductive Biology
Michal Zygarowicz, Joanna Kacperczyk‐Bartnik, Janusz Sierdziński, Cezary Wojtyła, Piotr Pierzyński, Grzegorz Mańka, Mariusz Kiecka, Robert Spaczyński, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokitaⴕ, Jakub Młodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Michał Lipa, Damian Warzecha, Mirosław Wielgoś, Ewa Koc‐Żórawska, Marcin Żórawski, Piotr Laudański,
Introduction: Endometriosis is an inflammatory-related reproductive age disease characterized by the presence of endometrial cells outside the uterine cavity. Current laboratory practice does not provide specific markers for detecting and assessing the advancement of endometriosis in either plasma or peritoneal fluid. The severity of disease is assessed in stages from I to IV based on the results of laparoscopic inspection. The protein annexin A2 (ANXA2) has been reported to be associated with inflammatory ...
Tópico(s): Uterine Myomas and Treatments
2023 - Dove Medical Press | Journal of Inflammation Research