Book.
1940 - Gale Group | World Scholar Latin America
Angel Matías Sanchez, Marina Inés Flamini, Xiaodong Fu, Paolo Mannella, Maria Silvia Giretti, Lorenzo Goglia, Andrea Riccardo Genazzani, Tommaso Simoncini,
Estrogens are important regulators of neuronal cell morphology, and this is thought to be critical for gender-specific differences in brain function and dysfunction. Dendritic spine formation is dependent on actin remodeling by the WASP-family verprolin homologous (WAVE1) protein, which controls actin polymerization through the actin-related protein (Arp)-2/3 complex. Emerging evidence indicates that estrogens are effective regulators of the actin cytoskeleton in various cell types via rapid, extranuclear ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2009 - Oxford University Press | Molecular Endocrinology
Book.
1943 - Gale Group | World Scholar Latin America
Paolo Mannella, Angel Matías Sanchez, Maria Silvia Giretti, Andrea Riccardo Genazzani, Tommaso Simoncini,
Sex steroids are important for brain function and protection. However, growing evidence suggests that these actions might depend on the timing of exposure to steroids. We have studied the effects of steroid administration on the survival of neural cells and we have partially characterized the possible mechanisms. The effect of a 24 h pre-treatment with 17β-estradiol or 17β-estradiol plus progesterone or medroxyprogesterone acetate on the toxic action of l-glutamate was used to test the experimental ...
Tópico(s): Neuroscience and Neuropharmacology Research
2009 - Elsevier BV | Steroids
Angel Matías Sanchez, Tommaso Simoncini,
Cell morphology is controlled by a complex and redundant array of intracellular signaling pathways devoted to the regulation of the actin cytoskeleton and of its relationship with the cell membrane and the extracellular matrix. Sex steroids are effective regulators of cell morphology and tissue organization, and recent evidence indicates that this is obtained through the regulation of the cytoskeleton. Intriguingly, many of these regulatory actions related to cell morphology are achieved through ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2009 - Elsevier BV | Steroids
Marina Inés Flamini, Angel Matías Sanchez, Lorenzo Goglia, Veronica Tosi, Andrea Riccardo Genazzani, Tommaso Simoncini,
Estrogen and selective estrogen receptor modulators (SERMs) differentially impact endometrial cell function, however, the biological basis of these differences is not established. Deregulated cell adhesion to the extracellular matrix, cell movement and invasion are related to endometrial disorders, such as endometriosis or endometrial cancer. Remodeling of the actin cytoskeleton is required to achieve cell adhesion and movement. Estrogen receptor (ER) regulates actin and cell membrane remodeling through ...
Tópico(s): Coagulation, Bradykinin, Polyphosphates, and Angioedema
2009 - Oxford University Press | Molecular Human Reproduction
Xiaodong Fu, Marina Inés Flamini, Angel Matías Sanchez, Lorenzo Goglia, Maria Silvia Giretti, A. R. Genazzani, Tommaso Simoncini,
The endothelial effects of progestogens are poorly investigated. Actin remodeling and cell movement are fundamental for endothelial function and are controlled by the actin-binding protein moesin. In this work, we studied the effects of progesterone and medroxyprogesterone acetate (MPA) on actin remodeling, moesin activation and cell movement in human endothelial cells. Our findings show that progesterone and MPA trigger a rapid endothelial actin rearrangement, with the formation of cortical actin ...
Tópico(s): Endometriosis Research and Treatment
2008 - Oxford University Press | Molecular Human Reproduction
Xiaodong Fu, Maria Silvia Giretti, Lorenzo Goglia, Marina Inés Flamini, Angel Matías Sanchez, Chiara Baldacci, Silvia Garibaldi, Régine Sitruk‐Ware, Andrea Riccardo Genazzani, Tommaso Simoncini,
Limited information is available on the effects of progestins on breast cancer progression and metastasis. Cell migration and invasion are central for these processes, and require dynamic cytoskeletal and cell membrane rearrangements for cell motility to be enacted.We investigated the effects of progesterone (P), medroxyprogesterone acetate (MPA), drospirenone (DRSP) and nestorone (NES) alone or with 17beta-estradiol (E2) on T47-D breast cancer cell migration and invasion and we linked some of these ...
Tópico(s): Angiogenesis and VEGF in Cancer
2008 - BioMed Central | BMC Cancer
Xiaodong Fu, Maria Silvia Giretti, Chiara Baldacci, Silvia Garibaldi, Marina Inés Flamini, Angel Matías Sanchez, Angiolo Gadducci, Andrea Riccardo Genazzani, Tommaso Simoncini,
Progesterone plays a role in breast cancer development and progression but the effects on breast cancer cell movement or invasion have not been fully explored. In this study, we investigate the actions of natural progesterone and of the synthetic progestin medroxyprogesterone acetate (MPA) on actin cytoskeleton remodeling and on breast cancer cell movement and invasion. In particular, we characterize the nongenomic signaling cascades implicated in these actions. T47-D breast cancer cells display ...
Tópico(s): Cell Adhesion Molecules Research
2008 - Public Library of Science | PLoS ONE
Marina Inés Flamini, Xiaodong Fu, Angel Matías Sanchez, Maria Silvia Giretti, Silvia Garibaldi, Lorenzo Goglia, Silvia Pisaneschi, Veronica Tosi, Andrea Riccardo Genazzani, Tommaso Simoncini,
Abstract Raloxifene (RAL) is a selective oestrogen receptor modulator (SERM) approved for the prevention and treatment of osteoporosis and for the prevention of breast cancer in postmenopausal women. However, little is known on the effects of this SERM on breast cancer cell metastasis, which is the main cause of morbidity and death. Cell movement is critical for local progression and distant metastasis of cancer cells. These processes rely on the dynamic control of the actin cytoskeleton and of cell ...
Tópico(s): HER2/EGFR in Cancer Research
2008 - Wiley | Journal of Cellular and Molecular Medicine
Marina Inés Flamini, Angel Matías Sanchez, Andrea Riccardo Genazzani, Tommaso Simoncini,
To explore the effects of 17β-estradiol (E(2)) on cytoskeletal remodeling and motility of endometrial stromal cells (ESC) and Ishikawa cells and to characterize the role of focal adhesion kinase (FAK) in these processes.In vitro study of cytoskeletal remodeling and cellular morphology and motility in ESC or Ishikawa cells.University research center.Endometrial samples obtained from women requiring endometrial biopsies.Treatments with E(2) and multiple inhibitors of signaling pathways.Activation of ...
Tópico(s): Reproductive System and Pregnancy
2010 - Elsevier BV | Fertility and Sterility
Xiaodong Fu, Lorenzo Goglia, Angel Matías Sanchez, Marina Inés Flamini, Maria Silvia Giretti, Veronica Tosi, Andrea R. Genazzani, Tommaso Simoncini,
While progesterone plays multiple roles in the process of breast development and differentiation, its role in breast cancer is less understood. We have shown previously that progestins stimulate breast cancer cell migration and invasion because of the activation of rapid signaling cascades leading to modifications in the actin cytoskeleton and cell membrane that are required for cell movement. In this study, we have investigated the effects of progesterone on the formation of focal adhesion (FA) ...
Tópico(s): HER2/EGFR in Cancer Research
2010 - Bioscientifica | Endocrine Related Cancer
Angel Matías Sanchez, Marina Inés Flamini, Chiara Baldacci, Lorenzo Goglia, Andrea Riccardo Genazzani, Tommaso Simoncini,
The ability of cancer cells to move and invade the surrounding environment is the basis of local and distant metastasis. Cancer cell movement requires dynamic remodeling of the cytoskeleton and cell membrane and is controlled by multiprotein complexes including focal adhesion kinase (FAK) or the Neural Wiskott-Aldrich Syndrome Protein (N-WASP). We show that 17β-estradiol induces phosphorylation of FAK and its translocation toward membrane sites where focal adhesion complexes are assembled. This process ...
Tópico(s): S100 Proteins and Annexins
2010 - Oxford University Press | Molecular Endocrinology
Angel Matías Sanchez, Marina Inés Flamini, Sara Zullino, Sandeep Gopal, Andrea R. Genazzani, Tommaso Simoncini,
Sex steroids play a key role in cell movement and tissue organization. Cell migration requires the integration of events that induce changes in cell structure such as protrusion, polarization and traction toward the direction of migration. These actions are driven by actin remodeling and are stabilized by the development of adhesion sites to extracellular matrix via transmembrane receptors linked to the actin cytoskeleton. Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that facilitates ...
Tópico(s): HER2/EGFR in Cancer Research
2010 - Oxford University Press | Molecular Human Reproduction
Lorenzo Goglia, Veronica Tosi, Angel Matías Sanchez, Marina Inés Flamini, Xiaodong Fu, Sara Zullino, Andrea R. Genazzani, Tommaso Simoncini,
The aim of this study is the identification of direct endothelial regulation by the androgens testosterone (T) and dihydrotestosterone (DHT). We tested the effects of T and DHT on nitric oxide (NO) synthesis and on tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) expression in human endothelial cells and in ovariectomized (OVX) rats. The results showed that at physiological concentrations T and DHT increase endothelial synthesis of NO. This depends on a rapid recruitment ...
Tópico(s): Estrogen and related hormone effects
2010 - Oxford University Press | Molecular Human Reproduction
Tommaso Simoncini, X-D. Fu, Antonio Caruso, Silvia Garibaldi, Chiara Baldacci, Maria Silvia Giretti, Paolo Mannella, Marina Inés Flamini, Angel Matías Sanchez, Andrea R. Genazzani,
Progestins have actions on the cardiovascular system, which depend on the structure as well as on receptor binding characteristics. Drospirenone (DRSP) is a progestin that uniquely interferes with the signaling of the mineralocorticoid receptor (MR). Hormone therapy containing DRSP results in blood pressure reduction in hypertensive post-menopausal women. We describe the effects of DRSP on endothelial nitric oxide (NO) synthesis and compare them with those of progesterone (P) and of medroxyprogesterone ...
Tópico(s): Menopause: Health Impacts and Treatments
2007 - Oxford University Press | Human Reproduction
Angel Matías Sanchez, Marina Inés Flamini, Kinga Polak, G. Palla, Stefania Spina, Paolo Mannella, Alessandro D. Genazzani, Tommaso Simoncini,
Cell morphology and its interaction with the extracellular environment are integrated processes involving a number of intracellular controllers orchestrating cytoskeletal proteins and their interaction with the cell membrane and anchorage proteins. Sex steroids are effective regulators of cell morphology and tissue organisation, and recent evidence indicates that this is obtained through the regulation of the actin cytoskeleton. Intriguingly, many of these regulatory actions related to cell morphology ...
Tópico(s): Menopause: Health Impacts and Treatments
2011 - Wiley | Journal of Neuroendocrinology
Silvia Pisaneschi, Francesca Anna Letizia Strigini, Angel Matías Sanchez, Silvia Begliuomini, Elena Casarosa, Andrea Ripoli, Paolo Ghirri, Antonio Boldrini, Bruno Fink, Andrea R. Genazzani, Flavio Coceani, Tommaso Simoncini,
Background Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric ...
Tópico(s): Neonatal Respiratory Health Research
2012 - Public Library of Science | PLoS ONE
Marina Inés Flamini, Gisel V. Gauna, Mayra L. Sottile, Silvina B. Nadin, Angel Matías Sanchez, Laura M. Vargas‐Roig,
Abstract Breast cancer is the most common malignancy in women and the appearance of distant metastases produces the death in 98% of cases. The retinoic acid receptor β ( RAR β) is not expressed in 50% of invasive breast carcinoma compared with normal tissue and it has been associated with lymph node metastasis. Our hypothesis is that RAR β protein participates in the metastatic process. T47D and MCF 7 breast cancer cell lines were used to perform viability assay, immunobloting, migration assays, RNA ...
Tópico(s): Chemokine receptors and signaling
2014 - Wiley | Journal of Cellular and Molecular Medicine
Angel Matías Sanchez, Marina Inés Flamini, Andrea Riccardo Genazzani, Tommaso Simoncini,
Sex steroids are important regulators of neuronal cell morphology, and this is critical for gender differences in brain function and dysfunction. Neuronal morphology is controlled by multiprotein complexes including moesin (a member of the ezrin/radixin/moesin family), focal adhesion kinase (FAK), or the Wiskott-Aldrich syndrome protein-family verprolin homologous (WAVE1) protein, controlling dynamic remodeling of the cytoskeleton and cell membrane. We investigated the actions of natural progesterone ( ...
Tópico(s): HER2/EGFR in Cancer Research
2013 - Oxford University Press | Molecular Endocrinology
Eric Padron, Guillermo Garcia‐Manero, Mrinal M. Patnaik, Raphaël Itzykson, Terra L. Lasho, Aziz Nazha, Raajit K. Rampal, Matias Sanchez, Elias Jabbour, Najla H Al Ali, Zachary Thompson, Simona Colla, Pierre Fenaux, Hagop M. Kantarjian, Sally Killick, Mikkael A. Sekeres, Alan F. List, Francesco Onida, Rami S. Komrokji, Ayalew Tefferi, Éric Solary,
Abstract Since its reclassification as a distinct disease entity, clinical research efforts have attempted to establish baseline characteristics and prognostic scoring systems for chronic myelomonocytic leukemia (CMML). Although existing data for baseline characteristics and CMML prognostication have been robustly developed and externally validated, these results have been limited by the small size of single-institution cohorts. We developed an international CMML data set that included 1832 cases ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2015 - Springer Nature | Blood Cancer Journal
Jorge Eduardo Shortrede, Ivonne Uzair, Flavia Judith Neira, Marina Inés Flamini, Angel Matías Sanchez,
Breast cancer is the major cause of cancer-related death in women. Its treatment is particularly difficult when metastasis occurs. The ability of cancer cells to move and invade the surrounding environment is the basis of local and distant metastasis. Cancer cells are able to remodel the actin cytoskeleton, which requires the recruitment of numerous structural and regulatory proteins that modulate actin filaments dynamics, including Paxillin or the Neural Wiskott-Aldrich Syndrome Protein (N-WASP). ...
Tópico(s): Estrogen and related hormone effects
2016 - Elsevier BV | Molecular and Cellular Endocrinology
Angel Matías Sanchez, Marina Inés Flamini, Eleonora Russo, Elena Casarosa, Simone Pacini, Mario Petrini, Andrea Riccardo Genazzani, Tommaso Simoncini,
Reproductive hormones influence breast cancer development and progression. While the actions of sex steroids in this setting are established, tentative evidence suggests that follicle-stimulating hormone (FSH) and luteinizing hormone (LH) may also play a role, yet this remains elusive. We here identify that T-47D breast cancer cells express functional receptors for FSH and LH, and that these hormones regulate breast cancer cell motility and invasion through the control of the actin cytoskeleton and ...
Tópico(s): Reproductive Biology and Fertility
2016 - Elsevier BV | Molecular and Cellular Endocrinology
I.F. Sáez del Bosque, Wenzhong Zhu, Torsten Howind, A. Matías, Agustín Matías-Sánchez, César Medina,
Tópico(s): Concrete and Cement Materials Research
2017 - Elsevier BV | Cement and Concrete Composites
Marina Inés Flamini, Ivonne Uzair, Gisela E. Pennacchio, Flavia Judith Neira, Joselina Magali Mondaca, F. Darío Cuello-Carrión, Graciela A. Jahn, Tommaso Simoncini, Angel Matías Sanchez,
Thyroid hormones (TH) play a fundamental role in diverse processes, including cellular movement. Cell migration requires the integration of events that induce changes in cell structure towards the direction of migration. These actions are driven by actin remodeling and stabilized by the development of adhesion sites to extracellular matrix via transmembrane receptors linked to the actin cytoskeleton. Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that promotes cell migration and invasion ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2017 - Springer Science+Business Media | Hormones and Cancer
Susana López‐Querol, Juana Arias-Trujillo, Maria GM-Elipe, Agustín Matías-Sánchez, Blas Cantero,
The improvement reached on the compaction and bearing capacity of aeolian sand collected in Jeddah (Saudi Arabia) after its stabilization with Portland cement is evaluated, comparing the behavior for both treated and untreated samples. With the aim of using this type of soil in the construction of embankments for road or railway applications, the results obtained have been evaluated in terms of maximum dry density, optimum moisture content (compaction test) and bearing capacity (CBR). Special attention ...
Tópico(s): Geotechnical Engineering and Analysis
2017 - Elsevier BV | Construction and Building Materials
Eva Gómez-Llanos, Pablo Durán‐Barroso, Agustín Matías-Sánchez,
From ecological and economic perspectives, current demands on freshwater management require the assessment of human water resource activities and urban water cycle impacts. The need to measure and control the quality of water returned to the environment is critical to evaluations of the efficiencies and sustainable management of wastewater treatment plants (WWTPs). This paper proposes a management assessment framework based on blue and grey water footprint (WF) to study the treatment and disposal ...
Tópico(s): Wastewater Treatment and Reuse
2018 - Elsevier BV | Journal of Cleaner Production
Blas Cantero, I.F. Sáez del Bosque, A. Matías, Agustín Matías-Sánchez, César Medina,
Dissociating economic growth from the use of natural resources is imperative to the sustainable development of the construction industry. The use of secondary raw materials by processing and managing construction and demolition waste (C&DW) is one of the major challenges to transition to a circular economy. This study assessed the effect of simultaneously using cement additioned with the ceramic (fired clay-based) fraction of C&DW and recycled mixed aggregate (RMA) in concrete manufacture by analysing ...
Tópico(s): Innovations in Concrete and Construction Materials
2019 - Springer Science+Business Media | Archives of Civil and Mechanical Engineering
Juana Arias-Trujillo, Agustín Matías-Sánchez, Blas Cantero, Susana López‐Querol,
An experimental investigation, aimed at evaluating the improvement of aeolian sand (from Saudi Arabia) when treated with low dosages of a vinyl acrylic (a polymer emulsion), is reported in this paper. Special attention is devoted to the influence of the lateral confinement, particularly in terms of compaction and bearing capacity (represented by CBR), for which a modification of the standard test has been developed trying to simulate extreme confinement conditions. Experimental results demonstrate ...
Tópico(s): Geotechnical Engineering and Analysis
2019 - Elsevier BV | Construction and Building Materials
Ivonne Uzair, Jeremias Conte Grand, Marina Inés Flamini, Angel Matías Sanchez,
The thyroid hormone triiodothyronine (T3) plays a fundamental role in growth regulation, differentiation, metabolism and cellular movement. These processes are particularly important considering that deregulation of T3 levels could promote abnormal responsiveness of mammary epithelial cells, which may lead to the development and progression of breast cancer (BC). Once cells migrate and invade different tissues, BC metastasis is the main cause of cancer-related death because it is particularly difficult ...
Tópico(s): Growth Hormone and Insulin-like Growth Factors
2019 - Frontiers Media | Frontiers in Endocrinology