Jean H. Humphrey, Mduduzi N. N. Mbuya, Robert Ntozini, Lawrence H. Moulton, Rebecca J. Stoltzfus, Naume V. Tavengwa, Kuda Mutasa, Florence D. Majo, Batsirai Mutasa, Goldberg Mangwadu, Cynthia Chasokela, Ancikaria Chigumira, Bernard Chasekwa, Laura E. Smith, James M. Tielsch, Andrew D. Jones, Amee R. Manges, John A. Maluccio, Andrew J. Prendergast, Jean H. Humphrey, Andrew D. Jones, Amee R. Manges, Goldberg Mangwadu, John A. Maluccio, Mduduzi N. N. Mbuya, Lawrence H. Moulton, Robert Ntozini, Andrew J. Prendergast, Rebecca J. Stoltzfus, James M. Tielsch, Cynthia Chasokela, Ancikaria Chigumira, William Heylar, Preston Hwena, George Kembo, Florence D. Majo, Batsirai Mutasa, Kuda Mutasa, Philippa Rambanepasi, Virginia Sauramba, Naume V. Tavengwa, Franne Van Der Keilen, Chipo Zambezi, Dzivaidzo Chidhanguro, Dorcas Chigodora, Joseph Chipanga, Grace Gerema, Tawanda Magara, Mandava Mandava, Tafadzwa Mavhudzi, Clever Mazhanga, Grace Muzaradope, Marian Mwapaura, Simon Phiri, Alice Tengende, Cynthia Banda, Bernard Chasekwa, Leah Chidamba, Theodore Chidawanyika, Elisha Chikwindi, Lovemore K. Chingaona, Courage K. Chiorera, Adlight Dandadzi, Margaret Govha, Hlanai Gumbo, Karen T. Gwanzura, Sarudzai Kasaru, Rachel Makasi, Alois M. Matsika, Diana Maunze, Exevia Mazarura, Eddington Mpofu, Johnson Mushonga, Tafadzwa E. Mushore, Tracey Muzira, Netsai Nembaware, Sibongile Nkiwane, Penias Nyamwino, Sandra Rukobo, Thompson Runodamoto, Shepherd Seremwe, Pururudzai Simango, Joice Tome, Blessing Tsenesa, Umali Amadu, Beauty Bangira, Daniel Chiveza, Priscilla Hove, Horaiti A. Jombe, Didymus Kujenga, Lenin Madhuyu, Prince M Makoni, Naume Maramba, Betty Maregere, Ellen Marumani, Elisha Masakadze, Phathisiwe Mazula, Caroline Munyanyi, Grace Musanhu, Raymond C. Mushanawani, Sibongile Mutsando, Felicia Nazare, Moses Nyarambi, Wellington Nzuda, Trylife Sigauke, Monica Solomon, Tendai Tavengwa, Farisai Biri, Misheck Chafanza, Cloud Chaitezvi, Tsundukani Chauke, Collen Chidzomba, Tawanda Dadirai, Clemence Fundira, Athanasios C. Gambiza, Tatenda Godzongere, Maria Kuona, Tariro Mafuratidze, Idah Mapurisa, Tsitsi Mashedze, Nokuthula Moyo, Charles Musariri, Matambudzo Mushambadope, Tawanda R. Mutsonziwa, Augustine Muzondo, Rudo Mwareka, Juleika Nyamupfukudza, Baven Saidi, Tambudzai Sakuhwehwe, Gerald Sikalima, Jenneth Tembe, Tapiwanashe E. Chekera, Owen Chihombe, Muchaneta Chikombingo, Tichaona Chirinda, Admire Chivizhe, Ratidzai Hove, Rudo Kufa, Tatenda F. Machikopa, Wilbert Mandaza, Liberty Mandongwe, Farirai Manhiyo, Emmanuel Manyaga, Peter Mapuranga, Farai S. Matimba, Patience Matonhodze, Sarah Mhuri, Joice Mike, Bekezela Ncube, Walter T. S. Nderecha, Munyaradzi Noah, Charles Nyamadzawo, Jonathan Penda, Asinje Saidi, Sarudzai Shonhayi, Clemence Simon, Monica Tichagwa, Rachael Chamakono, Annie Chauke, Andrew F. Gatsi, Blessing Hwena, Hillary Jawi, Benjamin Kaisa, Sithembile Kamutanho, Tapiwa Kaswa, Paradhi Kayeruza, Juliet Lunga, Nomatter Magogo, Daniel Manyeruke, Patricia Mazani, Fungai Mhuriyengwe, Farisai Mlambo, Stephen Moyo, Tawanda Mpofu, Mishelle Mugava, Yvonne Mukungwa, Fungai Muroyiwa, Eddington Mushonga, Selestino Nyekete, Tendai Rinashe, Kundai Sibanda, Milton Chemhuru, Jeffrey Chikunya, Vimbai F. Chikwavaire, Charity Chikwiriro, Anderson Chimusoro, Jotam Chinyama, Gerald Gwinji, Nokuthula Hoko-Sibanda, Rutendo Kandawasvika, Tendai Madzimure, Brian Maponga, Antonella Mapuranga, Joana Marembo, Luckmore Matsunge, Simbarashe Maunga, Mary Muchekeza, Monica Muti, Marvin Nyamana, Efa Azhuda, Urayai Bhoroma, Ailleen Biriyadi, Elizabeth Chafota, Angelline Chakwizira, Agness Chamhamiwa, Tavengwa Champion, Stella Chazuza, Beauty Chikwira, Chengeto Chingozho, Abigail Chitabwa, Annamary Dhurumba, Albert Furidzirai, Andrew Gandanga, Chipo Gukuta, Beauty Macheche, Bongani Marihwi, Barbara Masike, Eunice Mutangandura, Beatrice Mutodza, Angeline Mutsindikwa, Alice Mwale, Rebecca Ndhlovu, Norah Nduna, Cathrine Nyamandi, Elias Ruvata, Babra Sithole, Rofina Urayai, Bigboy Vengesa, Micheal Zorounye, Memory Bamule, Michael Bande, Kumbirai Chahuruva, Lilian Chidumba, Zvisinei Chigove, Kefas Chiguri, Susan Chikuni, Ruvarashe Chikwanda, Tarisai Chimbi, Micheal Chingozho, Olinia Chinhamo, Regina Chinokuramba, Chiratidzo Chinyoka, Xaviour Chipenzi, Raviro Chipute, Godfrey Chiribhani, Mary Chitsinga, Charles Chiwanga, Anamaria Chiza, Faith Chombe, Memory Denhere, Ephania Dhamba, Miriam Dhamba, Joyas Dube, Florence Dzimbanhete, Godfrey Dzingai, Sikhutele Fusira, Major Gonese, Johnson Gota, Kresencia Gumure, Phinias Gwaidza, Margret Gwangwava, Winnet Gwara, Melania Gwauya, Maidei Gwiba, Joyce Hamauswa, Sarah Hlasera, Eustina Hlukani, Joseph Hotera, Lovemore Jakwa, Gilbert Jangara, Micheal Janyure, Christopher Jari, Duvai Juru, Tabeth Kapuma, Paschalina Konzai, Moly Mabhodha, Susan Maburutse, Chipo Macheka, Tawanda Machigaya, Florence Machingauta, Eucaria Machokoto, Evelyn Madhumba, Learnard Madziise, Clipps Madziva, Mavis Madzivire, Mistake Mafukise, Marceline Maganga, Senzeni Maganga, Emmanuel Mageja, Miriam Mahanya, Evelyn Mahaso, Sanelisiwe Mahleka, Pauline Makanhiwa, Mavis Makarudze, Constant Makeche, Nickson Makopa,
BackgroundChild stunting reduces survival and impairs neurodevelopment. We tested the independent and combined effects of improved water, sanitation, and hygiene (WASH), and improved infant and young child feeding (IYCF) on stunting and anaemia in in Zimbabwe.MethodsWe did a cluster-randomised, community-based, 2 × 2 factorial trial in two rural districts in Zimbabwe. Clusters were defined as the catchment area of between one and four village health workers employed by the Zimbabwe Ministry of Health ...
Elsevier BV
Marya Plotkin, Katie M. Williams, Absolom Mbinda, Vivaldo Nunes Oficiano, Benard Nyauchi, Patrick Walugembe, Emily Keyes, Barbara Rawlins, Donna R. McCarraher, Otto Chabikuli,
Abstract Background The COVID-19 pandemic has disrupted the provision of essential reproductive, maternal, newborn, and child health (RMNCH) services in sub-Saharan Africa to varying degrees. Original models estimated as many as 1,157,000 additional child and 56,700 maternal deaths globally due to health service interruptions. To reduce potential impacts to populations related to RMNCH service delivery, national governments in Kenya, Mozambique, Uganda, and Zimbabwe swiftly issued policy guidelines ...
Tópico(s): Breastfeeding Practices and Influences
2022 - BioMed Central | BMC Public Health
Bicko Steve Juma, Asunta Mukami, Cecilia Mweu, Mathew Piero Ngugi, Wilton Mbinda,
Cassava is the world’s most essential food root crop, generating calories to millions of Sub-Saharan African subsistence farmers. Cassava leaves and roots contain toxic quantities of the cyanogenic glycoside linamarin. Consumption of residual cyanogens results in cyanide poisoning due to conversion of the cyanogens to cyanide in the body. There is a need for acyanogenic cassava cultivars in order for it to become a consistently safe and acceptable food, and commercial crop. In recent years, the ...
Tópico(s): GABA and Rice Research
2022 - Frontiers Media | Frontiers in Plant Science
Salinity stress is a major environmental impediment affecting the growth and production of crops. Finger millet is an important cereal grown in many arid and semi-arid areas of the world characterized by erratic rainfall and scarcity of good-quality water. Finger millet salinity stress is caused by the accumulation of soluble salts due to irrigation without a proper drainage system, coupled with the underlying rocks having a high salt content, which leads to the salinization of arable land. This ...
Tópico(s): Polysaccharides and Plant Cell Walls
2021 - Frontiers Media | Frontiers in Plant Science
Wilton Mbinda, Agnes Kavoo, Fredah Maina, Margaret Odeph, Cecilia Mweu, Nzilani Naomi, Mathew Piero Ngugi,
Abstract Background Finger millet blast disease, caused by Pyricularia oryzae , is a serious constrain of finger millet production which is threatening global food security especially to the resource poor smallholder farmers in arid and semi-arid regions. The disease adversely affects finger millet production and consumption due to its wide distribution and destruction in all finger millet growing areas of southern Asia and eastern Africa. Here, we present a study that investigated the occurrence, ...
Tópico(s): Plant Pathogenic Bacteria Studies
2021 - BioMed Central | CABI Agriculture and Bioscience
Wilton Mbinda, Hosea Isanda Masaki,
Climate change has significantly altered the biodiversity of crop pests and pathogens, posing a major challenge to sustainable crop production. At the same time, with the increasing global population, there is growing pressure on plant breeders to secure the projected food demand by improving the prevailing yield of major food crops. Finger millet is an important cereal crop in southern Asia and eastern Africa, with excellent nutraceutical properties, long storage period, and a unique ability to ...
Tópico(s): Wheat and Barley Genetics and Pathology
2021 - Frontiers Media | Frontiers in Plant Science
Mina C. Hosseinipour, Craig Innes, Sarita Naidoo, Philipp Mann, Julia Hütter, Gita Ramjee, Modulakgotla Sebe, Lucas Maganga, Michael E. Herce, Allan C. deCamp, Kyle Marshall, One Dintwe, Erica Andersen‐Nissen, Georgia D. Tomaras, Nonhlanhla N. Mkhize, Lynn Morris, Ryan L. Jensen, Maurine D. Miner, Giuseppe Pantaleo, S.-W Ding, Olivier Van Der Meeren, Susan W. Barnett, M. Juliana McElrath, Lawrence Corey, James G. Kublin, Nicole Frahm, Barbara Metch, Marguerite Koutsoukos, Stewart Reid, Bupe Sichalwe, Mah Asombang, Christine Namakobo, Sam Mundia, Lumbwe Banda, Joyce Mapanza, Jacinta Shilimi, Emmanuel Kapesa, Abisai Kisinda, Cornelia Lueer, Lilian Njovu, Wiston William, Faith Mlagalila, Elizabeth Ntapara, Willhelmina Olomi, Nnhamo Chiwerengo, Revocatus Kunambi, Bahati Myombe, Rosemary Mwilinga, Neema Mbinda, Joyce Masala, Joseph Mapunda, On Ho, Denelle Reilly, Liz Briesemeister, Marianne Hansen, Jill Zeller, Simbarashe Takuva, Caroline Brackett, Jack Heptinstall, Kelly E. Seaton, David Beaumont, Lu Zhang, Sheetal Sawant, Marcella Sarzotti‐Kelsoe, Tandile Hermanus, Valerie Bekker, Stephen De Rosa, Saleha Omarjee, Stephany Wilcox, Shamiska Rohith, Asiphe Basethi, R Da Costa Noble, Daryl E. Morris,
Abstract Background The Pox-Protein Public-Private Partnership is performing a suite of trials to evaluate the bivalent subtype C envelope protein (TV1.C and 1086.C glycoprotein 120) vaccine in the context of different adjuvants and priming agents for human immunodeficiency virus (HIV) type 1 (HIV-1) prevention. Methods In the HIV Vaccine Trials Network 111 trial, we compared the safety and immunogenicity of DNA prime followed by DNA/protein boost with DNA/protein coadministration injected intramuscularly ...
Tópico(s): HIV/AIDS drug development and treatment
2020 - Oxford University Press | Clinical Infectious Diseases
Mbinda Jonathan Kaleli, Paul Kuria Kamweru, Joel M. Gichumbi, F. G. Ndiritu,
Rice husk (RH) is an agricultural waste, and easily available in rice growing areas. The husk is mostly burnt as a way of getting rid of it. The ash obtained after burning or calcination may have economic application, mainly dependent on its properties. These properties in turn depend on the calcination method. However, for commercial viability, and for many applications, the calcination method should not only be as simple as possible but also cheap. This study characterized the elemental composition, ...
Tópico(s): Coal and Its By-products
2020 - Academic Journals | Journal of Chemical Engineering and Materials Science
Asunta Mukami, Alex Ngetich, Easter D. Syombua, Richard Oduor, Wilton Mbinda,
Finger millet is an important cereal that is grown in semi-arid and arid regions of East-Africa. Salinity stress is a major environmental impediment for the crop growth and production. This study aimed to understand the physiological and biochemical responses to salinity stress of six Kenyan finger millet varieties (GBK043137, GBK043128, GBK043124, GBK043122, GBK043094, GBK043050) grown across different agroecological zones under NaCl-induced salinity stress (100, 200 and 300 mM NaCl). Seeds were ...
Tópico(s): Leaf Properties and Growth Measurement
2020 - Springer Science+Business Media | Physiology and Molecular Biology of Plants
Mary Wandia Kivata, Margaret Mbuchi, Fredrick Eyase, Wallace Bulimo, Cecilia Kyany’a, Valerie Oundo, Wilton Mbinda, Willy Sang, Ben Andagalu, Olusegun O. Soge, R. Scott McClelland, John T. Distelhorst,
Abstract Background Treatment of gonorrhea is complicated by the development of antimicrobial resistance in Neisseria gonorrhoeae (GC) to the antibiotics recommended for treatment . Knowledge on types of plasmids and the antibiotic resistance genes they harbor is useful in monitoring the emergence and spread of bacterial antibiotic resistance. In Kenya, studies on gonococcal antimicrobial resistance are few and data on plasmid mediated drug resistance is limited. The present study characterizes plasmid ...
Tópico(s): Bacterial Infections and Vaccines
2020 - BioMed Central | BMC Infectious Diseases
Mary Wandia Kivata, Margaret Mbuchi, Fredrick Eyase, Wallace Bulimo, Cecilia Kyany’a, Valerie Oundo, Simon Wachira Muriithi, Ben Andagalu, Wilton Mbinda, Olusegun O. Soge, R. Scott McClelland, Willy Sang, James D. Mancuso,
Phenotypic fluoroquinolone resistance was first reported in Western Kenya in 2009 and later in Coastal Kenya and Nairobi. Until recently gonococcal fluoroquinolone resistance mechanisms in Kenya had not been elucidated. The aim of this paper is to analyze mutations in both gyrA and parC responsible for elevated fluoroquinolone Minimum Inhibitory Concentrations (MICs) in Neisseria gonorrhoeae (GC) isolated from heterosexual individuals from different locations in Kenya between 2013 and 2017. Antimicrobial ...
Tópico(s): Syphilis Diagnosis and Treatment
2019 - BioMed Central | BMC Microbiology
Asunta Mukami, Alex Ngetich, Cecilia Mweu, Richard Oduor, Mutemi Muthangya, Wilton Mbinda,
Drought is the most perilous abiotic stress that affects finger millet growth and productivity worldwide. For the successful production of finger millet, selection of drought tolerant varieties is necessary and critical stages under drought stress, germination and early seedling growth, ought to be fully understood. This study investigated the physiological and biochemical responses of six finger millet varieties (GBK043137, GBK043128, GBK043124, GBK043122, GBK043094 and GBK043050) under mannitol- ...
Tópico(s): Seed Germination and Physiology
2019 - Springer Science+Business Media | Physiology and Molecular Biology of Plants
Salinity is among the most severe and widespread environmental constrains to global crop production, especially in arid and semi-arid climates and negatively affecting productivity of salt sensitive crop species. Breeding and selection of salt tolerant crop varieties is therefore necessary for sustainable plant productivity. Given that germination and seeding phases are the most critical phase in the plant life cycle, this study aimed to evaluate seed germination potential and associated traits ...
Tópico(s): Food composition and properties
2019 - Sciencedomain International | Annual Research & Review in Biology
Wilton Mbinda, Omwoyo Ombori, Christina Dixelius, Richard Oduor,
Tópico(s): Aldose Reductase and Taurine
2018 - Springer Science+Business Media | Molecular Biotechnology
Michael Musila Ndile, David Nyaga Ngai, Jane Wanja Mbiri, Shardrack M Njagi, Wilton Mbinda, Mathew Piero Ngugi,
Medicinal plant Caesalpinia volkensii is used to treat Malaria, conjunctivitis, retinoblastoma, and eyelid swelling as well as gonorrhoea and bilharzia. Animal toxicity information on this plant is limited. This medicinal plant was collected in Embu County in Kenya to evaluate its Acute and sub-chronic toxicity using Wistar rats. The rats were orally administered with different doses of the plants extracts. They were weighed on first day and after every 7 days during treatment with the extract. ...
Tópico(s): Pharmacological Effects of Medicinal Plants
2017 - OMICS Publishing Group | Journal of Drug Metabolism & Toxicology
Magali Roques, Richard J. Wall, Alexander Douglass, Abhinay Ramaprasad, David Ferguson, Mbinda L. Kaindama, Lorenzo Brusini, Joshi Nimitray, Zineb Rchiad, Declan Brady, David S. Guttery, Sally P. Wheatley, Hiroyuki Yamano, Anthony A. Holder, Arnab Pain, Bill Wickstead, Rita Tewari,
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types ...
Tópico(s): Glycosylation and Glycoproteins Research
2015 - Public Library of Science | PLoS Pathogens
... African Wild Dog, Otjinene, Epukiro, Otjombinde, Omuramba Ua Mbinda, Eiseb and Ondjou communal conservancies, in order to ...
Tópico(s): Bryophyte Studies and Records
2014 - AOSIS | Koedoe
Majid Ezzati, Bernard M. Mbinda, Daniel M. Kammen,
Suspended particulate matter and carbon emissions from the combustion of biomass, in addition to their environmental consequences, have been causally associated with the incidence of respiratory and eye infections. Improved stoves offer the potential for emissions reduction. We compare the emissions of suspended particulate matter and carbon monoxide from traditional and improved biofuel stoves in Kenya under the actual conditions of household use. Data for analysis is from 137 14-h days of continuous ...
Tópico(s): COVID-19 impact on air quality
2000 - American Chemical Society | Environmental Science & Technology
Abstract Cassava is a major food crop for millions of people in Africa, Asia and South America, forming an essential food-security and income generation commodity for small-scale or subsistence farming communities. The storage root is the most important component of the crop that provides more calories than cereals. Immediately after harvest, cassava storage roots undergo complex biochemical and physiological changes known as postharvest physiological deterioration (PPD), which is influenced by genotype, ...
Tópico(s): Nematode management and characterization studies
2022 - BioMed Central | CABI Agriculture and Bioscience
Margaret Odeph, Wanjala Luasi Wycliffe, Kavoo Agnes, Mweu Cecilia, Ngugi Matthew, Maina Fredah, Nzilani Naomi, Mwema Mbinda Wilton,
Finger millet is a food crop that provides nutritional security and is climatically resilient for farming and agricultural diversification. However, its quality and yield remain low due to biotic and abiotic factors, the greatest of which is blast disease caused by Magnaporthe oryzae. We surveyed the occurrence, distribution and severity of finger millet blast in five counties, namely, Busia, Bungoma, Kisii, Machakos and Makueni, in Kenya. Blast disease occurrence was determined by observing disease ...
Tópico(s): Weed Control and Herbicide Applications
2020 - Academic Journals | African Journal of Plant Science
Morrisa Malkin, Alexandria K. Mickler, Theophilus O. Ajibade, Alexis Coppola, Eden Demise, Esinath Derera, Joy Otsanya Ede, Meghan Gallagher, Lucia Gumbo, Zorodzai Jakopo, Kristen Little, Absolom Mbinda, Gladwin Muchena, Nyaradzo Debra Muhonde, Khesiwe Ncube, Fifi Ogbondeminu, Shannon Pryor, Elsie Nzale Sang,
ABSTRACT Background: We describe how High Impact Practices (HIPs) in family planning (FP) were adapted across Kenya, Nigeria, and Zimbabwe to maintain access to services in response to the coronavirus disease (COVID-19) pandemic. Methods: Using a qualitative data collection tool structured around 3 HIP categories (service delivery, demand creation, and enabling environment), adaptations in FP programs during the pandemic were documented. We describe adaptations made to 3 specific HIPs: mobile outreach, community ...
Tópico(s): Adolescent Sexual and Reproductive Health
2022 - Johns Hopkins University Press | Global Health Science and Practice
This exploratory study describes high school students' perceptions of their parents' involvement in their education and in relation to school achievement. A new 12-item Parental Involvement Scale was used to measure parents' involvement in curricular and extracurricular activities and using exploratory analyses to estimate the scale's properties. Exploratory analysis resulted in the reduction of the 12 items to 8, with an internal consistency (Cronbach alpha) .82. Grade 12 science students indicated ...
Tópico(s): Family and Disability Support Research
2005 - SAGE Publishing | Psychological Reports
Wilton Mbinda, Colleta Musangi,
Calotropis procera it is known for traditional Kenyan medicinal system and it is used for it has previously been employed for treatment various diseases. This study aimed at examining methanolic extract of C. procera to establish antioxidant potential in vitro, total phenolic and flavonoid contents in order to identify potential sources of new antioxidants in food and pharmaceutical formulations. A comprehensive assessment on the antioxidant activity of stem back and root of C. procera by in vitro ...
Tópico(s): Phytochemistry and Biological Activities
2019 - | The Journal of Phytopharmacology
Wilton Mbinda, Christina Dixelius, Richard Oduor,
Drought stress often leads to reduced yields and is a perilous delimiter for expanded cultivation and increased productivity of sweet potato. Cell wall stabilization proteins have been identified to play a pivotal role in mechanical stabilization during desiccation stress mitigation in plants. They are involved in numerous cellular processes that modify cell wall properties to tolerate the mechanical stress during dehydration. This provides a plausible approach to engineer crops for enhanced stable ...
Tópico(s): Seed Germination and Physiology
2019 - Frontiers Media | Frontiers in Plant Science
Ngetich Alex, M. Cecilia, Mathew Piero Ngugi, Mukami Asunta, Ojulong Henry, Wilton Mbinda,
In the present study, an efficient protocol for somatic embryogenesis and plant regeneration was established in six finger millet varieties (GBK-043137, GBK-043128, GBK-043124, GBK-043122, GBK-043094 and GBK-043050). Shoot tips from 3 days in vitro grown plants were inoculated on MS supplemented with various concentrations and combinations of α-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and kinetin for callus induction and somatic embryogenesis. For ...
Tópico(s): Plant Genetic and Mutation Studies
2018 - Academic Journals | AFRICAN JOURNAL OF BIOTECHNOLOGY
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Mathew Piero Ngugi, Richard Oduor, Wilton Mbinda,
A high yield of isolated protoplast and reliable regeneration system are prerequisite for successful somatic hybridization and genome editing research. However, reproducible plant regeneration from protoplasts remains a bottleneck for many crops, including cassava. We evaluated several factors that influence isolation of viable protoplasts form leaf mesophyll, induction of embryogenic calli, and regeneration of plants in three cassava cultivars; Muchericheri, TMS60444 and Karibuni. A relatively ...
Tópico(s): Plant tissue culture and regeneration
2022 - Public Library of Science | PLoS ONE
Jane Wanja Mbiri, Sichangi Kasili, Patrick Kisangau, Michael Musila Ndile, Mathew Piero Ngugi, Wilton Mbinda,
Background: The barks of the medicinal plant, Terminalia brownii, are widely used in African folk medicine for the management of pain. However, this ethno-medicinal allegation has not been scientifically validated. This study was therefore designed to verify the antinociceptive potential of the methanolic bark extract of T. brownii in Wistar rats (Rattus novegicus). Methods: Fresh barks of T. brownii were obtained from Kitui County, Kenya with the guidance of a residential herbalist. The study used ...
Tópico(s): Complementary and Alternative Medicine Studies
2016 - OMICS Publishing Group | Journal of Pain & Relief
Steve Juma Bicko, M. Cecilia, Mathew Piero Ngugi, Wilton Mbinda,
Improved agricultural production of essential crops through advanced breeding is important for increasing access to nutritious food for the world's rapidly growing population, which is expected to reach 9.8 billion by 2050. Recent advancements in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein9 (CRISPR/Cas9) genome editing process, which uses single-guide RNA for genome editing, have made it easy, stable and efficient tool for targeted gene mutations, knockout ...
Tópico(s): GABA and Rice Research
2021 - Academic Journals | AFRICAN JOURNAL OF BIOTECHNOLOGY
Leanne K. Van der Weyde, Morulaganyi Kokole, Connie Modise, Balekanye Mbinda, Phale Seele, Rebecca Klein,
Livestock depredation can be devastating to both farmers and the species considered responsible if they are subsequently persecuted. Many proposed conflict solutions are limited in their uptake because they may be short-term, localised, expensive or species-specific. Livestock guarding dogs have been a successful solution in many parts of the world, however recommended imported breeds are generally expensive or inaccessible to many rural farmers. In this study, we report on a program placing local ...
Tópico(s): Rangeland Management and Livestock Ecology
2020 - | Journal of Vertebrate Biology
Wilton Mbinda, Sylvester Anami,
Sweetpotato is an important food crop in the world as well as in Kenya. Various fungal, and viral diseases are major constraints in its production and are currently threatening the sweetpotato production in sub-Saharan Africa. Genetic engineering offers significant potential for the crop’s genetic improvement. However, this is limited by the low efi¬Âciency and strong genotype dependency in tissue culture. This study aimed to establish an efficient somatic embryogenesis and plant regeneration system ...
Tópico(s): Sugarcane Cultivation and Processing
2016 - OMICS Publishing Group | Journal of Tissue Science & Engineering