Jorge P. López‐Alonso, Melisa Lázaro, David Gil-Cartón, Philip H. Choi, Alexandra Dodu, Liang Tong, Mikel Valle,
Abstract Pyruvate carboxylase (PC) is a tetrameric enzyme that contains two active sites per subunit that catalyze two consecutive reactions. A mobile domain with an attached prosthetic biotin links both reactions, an initial biotin carboxylation and the subsequent carboxyl transfer to pyruvate substrate to produce oxaloacetate. Reaction sites are at long distance, and there are several co-factors that play as allosteric regulators. Here, using cryoEM we explore the structure of active PC tetramers ...
Tópico(s): Photosynthetic Processes and Mechanisms
2022 - Nature Portfolio | Nature Communications
Patricia Julián, Andrey L. Konevega, Sjors H. W. Scheres, Melisa Lázaro, David Gil-Cartón, Wolfgang Wintermeyer, Marina V. Rodnina, Mikel Valle,
During protein synthesis, tRNAs and mRNA move through the ribosome between aminoacyl (A), peptidyl (P), and exit (E) sites of the ribosome in a process called translocation. Translocation is accompanied by the displacement of the tRNAs on the large ribosomal subunit toward the hybrid A/P and P/E states and by a rotational movement (ratchet) of the ribosomal subunits relative to one another. So far, the structure of the ratcheted state has been observed only when translation factors were bound to the ...
Tópico(s): RNA Research and Splicing
2008 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Xavier Querol, Andrés Alástuey, Sergio Rodrı́guez, Mar Viana, Begoña Artı́ñano, Pedro Salvador, E. Mantilla, Saúl García do Santos, Rosalía Fernández Patier, Jesús de la Rosa, Ana M. Sánchez de la Campa, Melisa Menéndez, Junsu Gil,
This paper summarises the results of a series of studies on the interpretation of time series of levels of total suspended particles (TSP) and particulate matter (PM, <10 μm) in six regions of Spain in the period 1996–2000. In addition to the local pollution events, high PM10 episodes are recorded during African dust outbreaks, regional atmospheric recirculation events (mainly in spring to autumn), and to a lesser extent, under the influence of European and Mediterranean long range transported air masses. ...
Tópico(s): Vehicle emissions and performance
2004 - Elsevier BV | The Science of The Total Environment
Mar Viana, Xavier Querol, Andrés Alástuey, J.I. Gil, Melisa Menéndez,
The effectiveness of combining principal component analysis (PCA) with multi-linear regression (MLRA) and wind direction data was demonstrated in this study. PM data from three grain-size fractions from a highly industrialised area in Northern Spain were analysed. Seven independent PM sources were identified by PCA: steel (Pb, Zn, Cd, Mn) and pigment (Cr, Mo, Ni) manufacture, road dust (Fe, Ba, Cd), traffic exhaust (P, OC + EC), regional-scale transport (NH4+, SO42-, V), crustal contributions (Al2O3, Sr, ...
Tópico(s): Vehicle emissions and performance
2006 - Elsevier BV | Chemosphere
Xavier Querol, Andrés Alástuey, Mar Viana, Sergio Rodrı́guez, Begoña Artı́ñano, Pedro Salvador, Saúl García do Santos, Rosalía Fernández Patier, Carolina Castillo, Jesús de la Rosa, Ana M. Sánchez de la Campa, Melisa Menéndez, J.I. Gil,
This work summarizes the results of a series of comprehensive studies on particulate matter (PM) carried out in Spain from 1999 to 2001. Monitoring sites were selected in accordance with different climatic and geographic conditions as well as anthropogenic influences, varying from rural background to urban curb-side sites. Measurements were carried out with gravimetric high-volume samplers and with automatic devices for different PM grain sizes, focusing on PM10 and PM2.5. A simultaneous meteorology ...
Tópico(s): Atmospheric chemistry and aerosols
2004 - Elsevier BV | Journal of Aerosol Science
Roberto Melero, Gretel Buchwald, Raquel Castaño, Monika Raabe, David Gil-Cartón, Melisa Lázaro, Henning Urlaub, Elena Conti, Óscar Llorca,
The nonsense-mediated mRNA decay (NMD) pathway is triggered upon assembly of the UPF surveillance complex near an exon junction complex (EJC). Cryo-EM studies have revealed the geometry of this transient assembly between the UPF complex and the EJC, which demonstrates how the UPF1 subunit elicits its RNA helicase activity toward the 3A end of the mRNA. Nonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance pathway that degrades aberrant mRNAs containing premature termination codons (PTCs). ...
Tópico(s): RNA modifications and cancer
2012 - Nature Portfolio | Nature Structural & Molecular Biology
Gorka Lasso, Linda Yu, David Gil-Cartón, Melisa Lázaro, Liang Tong, Mikel Valle,
The tetrameric enzyme pyruvate carboxylase (PC), a biotin-dependent carboxylase, produces oxaloacetate by two consecutive reactions that take place in distant active sites. Previous crystal structures revealed two different configurations for PC tetramers, the so-called symmetric and asymmetric, which were understood as characteristic molecular architectures for PC from different organisms. We have analyzed PC samples from Staphylococcus aureus while the enzyme generates oxaloacetate, expecting PC ...
Tópico(s): Advanced Electron Microscopy Techniques and Applications
2014 - Elsevier BV | Structure
Gil Lemos, Melisa Menéndez, Álvaro Semedo, Paula Camus, Mark Hemer, Mikhail Dobrynin, Pedro Miranda,
This study focuses on the assessment of systematic biases in wave climate simulations, exploring different bias correction methods commonly used for climate impact variables (e.g. precipitation, temperature), and on the analysis of bias corrected wave climate projections. Four different bias correction methods are analyzed, two of which are applied to an 8-member dynamic multi-forcing global wave climate ensemble, to mitigate the significant wave height (HS) bias. The GOW2 (Global Ocean Waves 2) global ...
Tópico(s): Coastal and Marine Dynamics
2019 - Elsevier BV | Global and Planetary Change
Gil Lemos, Melisa Menéndez, Álvaro Semedo, Pedro Miranda, Mark Hemer,
Tópico(s): Ocean Waves and Remote Sensing
2021 - Springer Science+Business Media | Climate Dynamics
Diana C. Fechner, Melisa J. Hidalgo, Juan Díaz, Raúl A. Gil, Roberto G. Pellerano,
Traceability of food has become an important issue. In particular, the traceability of natural products has become an important topic considering the high number of possible sources of environmental pollutants which these products are exposed during the production process. Chemical composition and organoleptic characteristics of honey are strongly influenced by its geographical origin. Currently, multivariate statistical techniques applied to multielement data are used to identify geographical origin ...
Tópico(s): Meat and Animal Product Quality
2019 - Elsevier BV | Food Bioscience
Héctor Lobeto, Álvaro Semedo, Melisa Menéndez, Gil Lemos, Rajesh Kumar, Adem Akpınar, Mikhail Dobrynin, Bahareh Kamranzad,
Abstract This study investigates the epistemic uncertainty associated with the wave propagation modeling in wave climate projections. A single-forcing, single-scenario, seven-member global wave climate projection ensemble is used, developed using three wave models with a consistent numerical domain. The uncertainty is assessed through projected changes in wave height, wave period, and wave direction. The relative importance of the wave model used and its internal parameterization are examined. The ...
Tópico(s): Oceanographic and Atmospheric Processes
2023 - IOP Publishing | Environmental Research Letters
Edgar Enrique Neri Castro, Melisa Bénard-Valle, Alejandro Alagón, Guillermo Gil, Jorge López de León, Miguel Borja,
El estudio de los venenos de serpientes mexicanas ha retomado importancia en los últimos cinco años. Se han caracterizado un número importante de venenos, que incluyen estudios sobre la variación intraespecífica de las especies de mayor importancia médica. Sin embargo, hay una enorme falta de reportes de casos clínicos y estudios sobre la potencia neutralizante de los antivenenos hacia el veneno de las especies mexicanas. En el presente trabajo se realizó una revisión sobre los estudios de los venenos ...
Tópico(s): Insect and Pesticide Research
2020 - National Autonomous University of Mexico | Revista Latinoamericana de Herpetología
Elisa Belén Cortés Zamora, Marta Mas Romero, María Teresa Tabernero Sahuquillo, Almudena Avendaño Céspedes, Fernando Andrés-Petrel, Cristina Gómez Ballesteros, Victoria Sánchez‐Flor Alfaro, Rita López-Bru, Melisa López-Utiel, Sara Cifuentes, Laura Plaza Carmona, Borja Gil García, Ana Pérez Fernández-Rius, Rubén Alcantud Córcoles, Belén Roldán García, Luis Romero Rizos, Pedro Manuel Sánchez‐Jurado, Carmen Luengo Márquez, Mariano Esbrí Víctor, Matilde León, Gabriel Ariza‐Zafra, Elena Martín Sebastiá, Esther Jiménez, Gema Paterna Mellinas, Esther Martínez‐Sánchez, Alicia Noguerón García, María Fe Ruiz García, Rafael García Molina, Juan de Dios Estrella Cazalla, Pedro Abizanda,
To analyze the psychological and functional sequelae of the COVID-19 pandemic among older adults living in long term care facilities (LTCFs).Cohort longitudinal study SETTING ANT PARTICIPANTS: A total of 215 residents ≥ 65 years without moderate-to-severe cognitive impairment, living in five LTCFs in Albacete (Spain).Baseline on-site data were collected between March - June 2020 and three-month follow-up between June to September 2020. Symptoms of depression, anxiety, posttraumatic stress disorder (PTSD), ...
Tópico(s): Long-Term Effects of COVID-19
2022 - Elsevier BV | American Journal of Geriatric Psychiatry
Isaiah Herrera, Melisa Hernández Febles, R. González Jorge, Elena Colino Gil, Ana Bordes Benítez, María José Pena López,
El objetivo del estudio fue conocer el patrón clínico-epidemiológico de la tos ferina en niños diagnosticados en un hospital pediátrico de la isla de Gran Canaria. Se revisaron retrospectivamente las historias clínicas de los pacientes con diagnóstico de infección por Bordetella pertussis mediante reacción en cadena de la polimerasa (PCR) en el período septiembre 2003-septiembre 2007. Se detectaron 49 casos de tos ferina, 47 en menores de 6 meses. La incidencia anual de hospitalización osciló entre ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2008 - Elsevier BV | Anales de Pediatría
Roberto Melero, S. Rajagopalan, Melisa Lázaro, Andreas C. Joerger, Tobias Brandt, Dmitry B. Veprintsev, Gorka Lasso, David Gil-Cartón, Sjors H. W. Scheres, J.M. Carazo, Alan R. Fersht, Mikel Valle,
The multidomain homotetrameric tumor suppressor p53 has two modes of binding dsDNA that are thought to be responsible for scanning and recognizing specific response elements (REs). The C termini bind nonspecifically to dsDNA. The four DNA-binding domains (DBDs) bind REs that have two symmetric 10 base-pair sequences. p53 bound to a 20-bp RE has the DBDs enveloping the DNA, which is in the center of the molecule surrounded by linker sequences to the tetramerization domain (Tet). We investigated by electron ...
Tópico(s): RNA modifications and cancer
2010 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Gil Lemos, Álvaro Semedo, Mark Hemer, Melisa Menéndez, Pedro Miranda,
Abstract The energy content of wind-waves is propagated across the oceans in the form of swell waves, the main drivers of long-term changes in coastal morphology and offshore hazards. A state-of-the-art swell tracking algorithm is applied to a global ensemble of CMIP5 dynamic wave climate projections, to assess future changes in remotely originated swell events towards the end of the 21st century, and how they propagate. The contribution of multiple wave generation areas is considered. It is found ...
Tópico(s): Tropical and Extratropical Cyclones Research
2021 - IOP Publishing | Environmental Research Letters
Gil Lemos, Álvaro Semedo, Mikhail Dobrynin, Melisa Menéndez, Pedro Miranda,
Abstract A quantile-based bias-correction method is applied to a seven-member dynamic ensemble of global wave climate simulations with the aim of reducing the significant wave height H S , mean wave period T m , and mean wave direction (MWD) biases, in comparison with the ERA5 reanalysis. The corresponding projected changes toward the end of the twenty-first century are assessed. Seven CMIP5 EC-EARTH runs (single forcing) were used to force seven wave model (WAM) realizations (single model), following the ...
Tópico(s): Climate variability and models
2020 - American Meteorological Society | Journal of Applied Meteorology and Climatology
... Gonçalves, Juanjo Fernández, Rosa Fernández, Benita Fernández Martínez, Melisa Fernández Puentes, Amelia Fonseca, Joaquím Fonseca, José Manuel Fraile, Isabel García, María Antonieta García, Alzira Gaspar, Joëlle Ghazarian, Flores Gil, Inez Luna Gil, Prazeres Giraldes, Paz Gómez, Célia ...
Tópico(s): Musicology and Musical Analysis
2008 - Taylor & Francis | Ethnomusicology Forum
Marta Mas Romero, Almudena Avendaño Céspedes, María Teresa Tabernero Sahuquillo, Elisa Belén Cortés Zamora, Cristina Gómez Ballesteros, Victoria Sánchez‐Flor Alfaro, Rita López Bru, Melisa López Utiel, Sara Cifuentes, Luz María Peña-Longobardo, Antonio Murillo Romero, Laura Plaza Carmona, Borja Gil García, Ana Pérez Fernández-Rius, Rubén Alcantud Córcoles, Belén Roldán García, Luis Romero Rizos, Pedro Manuel Sánchez Jurado, Matilde León, Pilar Atienzar Núñez, Alicia Noguerón García, María Fe Ruiz García, Rafael García Molina, Juan de Dios Estrella Cazalla, Juan Oliva, Pedro Abizanda,
Background/Objectives To analyze mortality, costs, residents and personnel characteristics, in six long-term care facilities (LTCF) during the outbreak of COVID-19 in Spain. Design Epidemiological study. Setting Six open LTCFs in Albacete (Spain). Participants 198 residents and 190 workers from LTCF A were included, between 2020 March 6 and April 5. Epidemiological data were also collected from six LTCFs of Albacete for the same period of time, including 1,084 residents. Measurements Baseline demographic, ...
Tópico(s): COVID-19 and healthcare impacts
2020 - Public Library of Science | PLoS ONE
Pablo Fernández-Millán, Melisa Lázaro, Şirin Cansız-Arda, Joachim M. Gerhold, Nina Rajala, Claus-A. Schmitz, Cristina Silva-Espiña, David Gil-Cartón, Pau Bernadó, Mikel Valle, Johannes N. Spelbrink, Marı́a Solà,
The mitochondrial replicative helicase Twinkle is involved in strand separation at the replication fork of mitochondrial DNA (mtDNA). Twinkle malfunction is associated with rare diseases that include late onset mitochondrial myopathies, neuromuscular disorders and fatal infantile mtDNA depletion syndrome. We examined its 3D structure by electron microscopy (EM) and small angle X-ray scattering (SAXS) and built the corresponding atomic models, which gave insight into the first molecular architecture ...
Tópico(s): DNA Repair Mechanisms
2015 - Oxford University Press | Nucleic Acids Research
Héctor Lobeto, Álvaro Semedo, Gil Lemos, Ali Dastgheib, Melisa Menéndez, Roshanka Ranasinghe, Jean‐Raymond Bidlot,
Abstract Coastal wave storms pose a massive threat to over 10% of the world’s population now inhabiting the low elevation coastal zone and to the trillions of $ worth of coastal zone infrastructure and developments therein. Using a ~ 40-year wave hindcast, we here present a world-first assessment of wind-wave storminess along the global coastline. Coastal regions are ranked in terms of the main storm characteristics, showing Northwestern Europe and Southwestern South America to suffer, on average, the ...
Tópico(s): Tropical and Extratropical Cyclones Research
2024 - Nature Portfolio | Scientific Reports
Laura Llorente, Antonio Casabella, Melisa Hernández Febles, Elena Colino Gil, Alicia Eisman Maraver, María José Pena López,
Describir el patrón epidemiológico y clínico de la infección por Bordetella pertussis (tosferina) en niños menores de un año hospitalizados en un hospital pediátrico de Gran Canaria. Se revisaron retrospectivamente las historias clínicas de los pacientes con diagnóstico microbiológico de infección por B. pertussis mediante reacción en cadena de la polimerasa, de enero de 2008 a diciembre de 2016. Se identificaron 110 pacientes, de los cuales 105 (95,4%) fueron menores de 6 meses y el 59,1% eran varones. ...
Tópico(s): Pneumonia and Respiratory Infections
2017 - Elsevier BV | Anales de Pediatría
Marina Pollán, Beatriz Pérez‐Gómez, Roberto Pastor‐Barriuso, Jesús Oteo, Miguel A. Hernán, Mayte Pérez‐Olmeda, Jose L Sanmartín, Aurora Fernández-García, Israel Cruz, Nerea Fernández de Larrea, Marta Molina, Francisco Rodríguez-Cabrera, Mariano Martín, Paloma Merino Amador, José León Paniagua, Juan F Muñoz-Montalvo, Faustino Blanco, Raquel Yotti, Faustino Blanco, Rodrigo Gutiérrez Fernández, Mariano Martín, Saturnino Mezcua Navarro, Marta Molina, Juan F Muñoz-Montalvo, Matías Salinero Hernández, Jose L Sanmartín, Manuel Cuenca‐Estrella, Raquel Yotti, José León Paniagua, Nerea Fernández de Larrea, José María Navarro‐Marí, Roberto Pastor‐Barriuso, Beatriz Pérez‐Gómez, Marina Pollán, Ana Avellón, Giovanni Fedele, Aurora Fernández-García, Jesús Oteo, Mayte Pérez‐Olmeda, Israel Cruz, Elena Fernández‐Martínez, Francisco Rodríguez-Cabrera, Miguel A. Hernán, Susana Padrones Fernández, José Manuel Rumbao Aguirre, José María Navarro‐Marí, Begoña Palop Borrás, Ana Belén Jiménez, Manuel Rodríguez‐Iglesias, Ana María Calvo Gascón, María Luz Lou Alcaine, Ignacio Donate Suárez, O. Alvarez, Mercedes Rodríguez Pérez, Margarita Cases Sanchís, Carlos Javier Villafáfila-Gomila, Lluís Carbó Saladrigas, Adoración Hurtado Fernández, Antonio Oliver, Elías Castro Feliciano, María Noemí González Quintana, José María Barrasa Fernández, María Araceli Hernández Betancor, Melisa Hernández Febles, Leopoldo Martín Martín, Luis-Mariano López López, Teresa Ugarte Miota, Inés De Benito Población, María Sagrario Celada Pérez, María Natalia Vallés Fernández, Tomás Maté, M. Arranz, Marta Domínguez-Gil González, Isabel Fernández-Natal, Gregoria Megías Lobón, Juan Luis Muñoz Bellido, Pilar Ciruela, Ariadna Mas i Casals, María Doladé, María Ángeles Marcos, Dúnia Pérez del Campo, Antonio Félix de Castro, Ramón Limón Ramírez, Maria Francisca Elías Retamosa, Manuela Rubio González, María Sinda Blanco Lobeiras, Alberto Fuentes Losada, Antonio Aguilera, Germán Bou, Yolanda Caro, Noemí Marauri, Luis Miguel Soria Blanco, Isabel del Cura-González, Montserrat Hernández Pascual, Roberto Alonso, Paloma Merino Amador, Natalia Cabrera Castro, Aurora Tomás Lizcano, Cristóbal Ramírez Almagro, M. Segovia Hernández, Nieves Ascunce Elizaga, María Ederra Sanz, Carmen Ezpeleta Baquedano, Ana Bustinduy Bascaran, Susana Tamayo, Luis Elorduy Otazua, Rebeca Benarroch Benarroch, Jesús Lopera Flores, Antonia Vázquez de la Villa,
BackgroundSpain is one of the European countries most affected by the COVID-19 pandemic. Serological surveys are a valuable tool to assess the extent of the epidemic, given the existence of asymptomatic cases and little access to diagnostic tests. This nationwide population-based study aims to estimate the seroprevalence of SARS-CoV-2 infection in Spain at national and regional level.Methods35 883 households were selected from municipal rolls using two-stage random sampling stratified by province ...
Tópico(s): SARS-CoV-2 and COVID-19 Research
2020 - Elsevier BV | The Lancet
Laura Llorente, Antonio Casabella, Melisa Hernández Febles, Elena Colino Gil, Alicia Eisman Maraver, María José Pena López,
Describe the epidemiological and clinical pattern of Bordetella pertussis infection (whooping cough) among hospitalised infants less than one year-old in a paediatric hospital in Gran Canaria.A retrospective review of the patient hospital records was performed, and recording only those with a microbiological diagnosis of pertussis infection detected using polymerase chain reaction, from January 2008 to December 2016.A total of 110 patients were identified, of which 105 (95.4%) were less than 6 months ...
Tópico(s): Pneumonia and Respiratory Infections
2018 - Elsevier BV | Anales de Pediatría (English Edition)
María Cristina Almazán, Griselda Noemí Copa, Juan José Lauthier, José F. Gil, Inés López Quiroga, Carlos L. Hoyos, Melisa Evangelina Díaz Fernández, Julio R. Nasser, Masataka Korenaga, Jorge D. Marco, Paola A. Barroso,
Leishmaniases are vector-borne diseases that in the Americas are distributed from southern United States to northern Argentina. The vectors for this disease are small dipterans known as sand flies that are usually identified morphologically by observing structures with taxonomic value; but it is time-consuming, laborious, and requires entomological expertise. Then, this work was aimed at identifying sand flies with molecular techniques, using the morphological identification as a reference technique, ...
Tópico(s): Insect Resistance and Genetics
2020 - Elsevier BV | Acta Tropica
Magdalena Bosio Bonet, Melisa Corball, Rocío Gil Redondo,
Tópico(s): Dermatologic Treatments and Research
2023 - Elsevier BV | Piel
Jorge P. López‐Alonso, Melisa Lázaro, David Gil-Cartón, Philip H. Choi, Alexandra Dodu, Liang Tong, Mikel Valle,
Tópico(s): Biotin and Related Studies
2022 - Nature Portfolio | Nature Communications
Kelly Johana Corredor, Sara Manuela Gil, Melisa Naranjo Vanegas,
Introducción: El cáncer de mama (CM) es una enfermedad heterogénea, clasificada en tres subtipos intrínsecos principales: receptores hormonales positivos (RH+), con sobreexpresión del oncogén HER2 y triples negativos (TN), cada uno de ellos con hallazgos imagenológicos representativos. Objetivo: Caracterizar y clasificar los hallazgos de los subtipos intrínsecos del CM utilizando imágenes de mamografía con medio de contraste endovenoso. Metodología: Se realizó un análisis descriptivo de una submuestra ...
Tópico(s): Global Cancer Incidence and Screening
2023 - | Revista colombiana de radiología
María Cristina Almazán, Silvana P. Cajal, Melisa Díaz-Fernández, Marisa Juaréz, Marcelo Quipildor, Guillermo Portal, Valeria Tejerina, Carlos Vargas, Griselda Noemí Copa, José F. Gil, Rubén O. Cimino, Alejandro Krolewiecki,
ABSTRACT. American tegumentary leishmaniasis (ATL) is a neglected tropical disease affecting the skin and mucosa. American tegumentary leishmaniasis due to Leishmania ( Viannia ) braziliensis is endemic in Argentina, where the Department of Oran is a hyperendemic focus. All cases of ATL with laboratory confirmation evaluated at a referral center in Oran city between 1985 and 2019 were analyzed retrospectively. Information from cases included clinical form, lesion size and number, time of evolution, and ...
Tópico(s): Research on Leishmaniasis Studies
2023 - American Society of Tropical Medicine and Hygiene | American Journal of Tropical Medicine and Hygiene
Montoya Martínez Jj, José Miguel Rubio, Y. Ouahid, Analía Melisa López, Antonio Madejón, Gil-Garcia Ai, Hannam Rj, Butler Hre, P. Castan,
Background and aims: Quick, user-friendly, and sensitive diagnostic tools are the keys to controlling the spread of the SARS-CoV-2 pandemic in the new epidemiologic landscape. The aim of this work is to characterize a new COVID-19 antigen test that uses an innovative chromatographic Affimer®-based technology designed for the qualitative detection of SARS-CoV-2 antigen. As rapid technology to detect COVID-19, the test was extensively characterized in vitro. Once the analytical parameters of performance ...
Tópico(s): Biosensors and Analytical Detection
2021 - | Journal of Biology and Today s World