Produção Nacional
Revisado por pares
Ariane Zamoner, Alessandra Nejar Bruno, Émerson André Casali, Patrícia Frasson Corbelini, Gabriela Placoná Diniz, Maria Luiza Morais Barreto‐Chaves, Fátima Regina Mena Barreto Silva, João José Freitas Sarkis, Regina Pessoa‐Pureur,
The Sertoli cells play an essential role in the maintenance and control of spermatogenesis. The ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) and 5′-nucleotidase activities can modulate the extracellular adenine nucleotide levels, controlling nucleotide-mediated signaling events in Sertoli cells. Since thyroid hormones (TH) and adenine nucleotides and nucleosides play important modulatory roles in Sertoli cell proliferation and differentiation, the aim of our study was to investigate ...
Tópico(s): Folate and B Vitamins Research
2006 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Ariane Zamoner, Carine Royer, Kátia Padilha Barreto, Regina Pessoa‐Pureur, Fátima Regina Mena Barreto Silva,
Thyroid hormones (TH) play important roles in brain development. Although most of the nongenomic actions of TH are known to be calcium-dependent, the effects of 3,5,3′-triiodo-l-thyronine (T3) or thyroxine (T4) on calcium influx in cerebral cortex of rats are not clear. In this study we investigate some mechanisms involved in the effect of T3 and T4 on Ca2+ uptake in slices of cerebral cortex from 10-day-old male rats. Results indicated 10−6 M T3 or 10−7 M T4 was able to increase 45Ca2+ uptake after ...
Tópico(s): Ion channel regulation and function
2006 - Elsevier BV | Neuroscience Research
Produção Nacional
Revisado por pares
Karine Cunha Volpato, Danusa Menegaz, Laura D. Leite, Kátia Padilha Barreto, Euler de Vilhena Garcia, Fátima Regina Mena Barreto Silva,
The purpose of this study was to investigate the involvement of calcium in K+ currents and its effects on amino acid accumulation and on the membrane potential regulated by tri-iodo-L-thyronine (T3) in Sertoli cells. Immature rat testes were pre-incubated for 30 min in Krebs-Ringer bicarbonate buffer and incubated for 60 min in the presence of [14C]methylaminoisobutyric acid with and without T3 or T4 (dose-response curve). Specific channel blockers or chelating agents were added at different concentrations ...
Tópico(s): Pharmacological Effects and Toxicity Studies
2003 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Ariane Zamoner, Kátia Padilha Barreto, Danilo Wilhelm Filho, Fabíola Sell, Viviane Mara Woehl, Fátima Theresinha Costa Rodrigues Guma, Fátima Regina Mena Barreto Silva, Regina Pessoa‐Pureur,
Hyperthyroidism was induced in rats and somatic indices and metabolic parameters were analyzed in testis. In addition, the morphological analysis evidenced testes maturation and intense protein synthesis and processing, supporting the enhancement in vimentin synthesis in hyperthyroid testis. Furthermore, vimentin phosphorylation was increased, indicating an accumulation of phosphorylated vimentin associated to the cytoskeleton, which could be a consequence of the extracellular-regulated kinase (ERK) ...
Tópico(s): Reproductive Biology and Fertility
2007 - Elsevier BV | Molecular and Cellular Endocrinology
Produção Nacional
Revisado por pares
Ariane Zamoner, Kátia Padilha Barreto, Danilo Wilhelm Filho, Fabíola Sell, Viviane Mara Woehl, Fátima Theresinha Costa Rodrigues Guma, Regina Pessoa‐Pureur, Fátima Regina Mena Barreto Silva,
Congenital hypothyroidism was induced in rats by adding 0.05% 6-propyl-2-thiouracil in the drinking water from day 9 of gestation, and continually up to postnatal day 15. Structural alterations observed by light microscopy of seminiferous tubules and by transmission electron microscopy of Sertoli cells of treated animals were consistent with hypothyroid condition. Hypothyroidism was also associated with high phospho-p38 mitogen-activated protein kinase and decreased phospho-extracellular signal- ...
Tópico(s): Virus-based gene therapy research
2008 - Bioscientifica | Journal of Molecular Endocrinology
Produção Nacional
Revisado por pares
Hemily Batista-Silva, Keyla Rodrigues, Kieiv Resende Sousa de Moura, Nicolas Elie, Glen Van Der Kraak, Christelle Delalande, Fátima Regina Mena Barreto Silva,
This study investigated the in vitro and short-term in vivo effects of Bisphenol A (BPA) on testicular energy metabolism and morphology in the zebrafish (Danio rerio). Testes were incubated in vitro for 1 h or fish were exposed in vivo to BPA in the tank water for 12 h. Testicular lactate, glycogen and cholesterol were measured and 14C-deoxy-d-glucose uptake and activity of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. In addition, testis ...
Tópico(s): Toxic Organic Pollutants Impact
2021 - Elsevier BV | Reproductive Toxicology
Produção Nacional
Revisado por pares
Hemily Batista-Silva, Keyla Rodrigues, Kieiv Resende Sousa de Moura, Glen Van Der Kraak, Christelle Delalande-Lecapitaine, Fátima Regina Mena Barreto Silva,
This study investigated the acute in vitro effect of low-concentration bisphenol A (BPA) on calcium (45Ca2+) influx in zebrafish (Danio rerio) testis and examined whether intracellular Ca2+ was involved in the effects of BPA on testicular toxicity. In vitro studies on 45Ca2+ influx were performed in the testes after incubation with BPA for 30 min. Inhibitors were added 15 min before the addition of 45Ca2+ and BPA to testes to study the mechanism of action of BPA. The involvement of intracellular calcium ...
Tópico(s): Estrogen and related hormone effects
2020 - Elsevier BV | Ecotoxicology and Environmental Safety
Produção Nacional
Revisado por pares
Hemily Batista-Silva, Betina Fernanda Dambrós, Keyla Rodrigues, Patrícia A. Cesconetto, Ariane Zamoner, Kieiv Resende Sousa de Moura, Allisson Jhonatan Gomes Castro, Glen Van Der Kraak, Fátima Regina Mena Barreto Silva,
Bis(2-ethylhexyl)phthalate (BEHP) negatively affects testicular functions in different animal species, disturbing reproductive physiology and male fertility. The present study investigated the in vitro acute effect of BEHP on the mechanism of action of ionic calcium (Ca2+) homeostasis and energy metabolism. In addition, the effect of BEHP on oxidative stress was studied in vitro and in vivo in the testis of Danio rerio (D. rerio). Testes were treated in vitro for 30 min with 1 μM BEHP for 45Ca2+ influx ...
Tópico(s): Reproductive biology and impacts on aquatic species
2020 - Elsevier BV | Biochimie
Produção Nacional
Revisado por pares
Delsi Altenhofen, Gabrielle da Luz, Marisa Jádna Silva Frederico, Dalila Venzke, Mayara Brich, Silvana Virgínia Gagliotti Vigil, Tânia Sílvia Fröde, Carlos Eduardo Blanco Linares, Moacir Geraldo Pizzolatti, Fátima Regina Mena Barreto Silva,
Isoflavones widely distributed in plants prevent diabetes. This study investigated the in vivo and in vitro effect of 3',4'-dihydroxy-6″,6″,6″',6″'-tetramethylbis(pyrano[2″,3″:5,6::2″',3″':7,8]isoflavone (bis-pyrano prenyl isoflavone) on glucose homeostasis in hyperglycemic rats. The ethyl acetate fraction from aerial parts of Polygala molluginifolia that contain isoflavones was assayed on glucose tolerance, on in vitro maltase activity and on protein glycation. The isoflavone bis-pyrano prenyl isolated ...
Tópico(s): Carbohydrate Chemistry and Synthesis
2016 - Wiley | Journal of Cellular Biochemistry
Gavin Giovannoni, Jeffrey Cohen, Alasdair Coles, Hans-Peter Hartung, Eva Havrdová, Krzysztof Selmaj, David Margolin, Stephen Lake, Susan M. Kaup, Michael Panzara, D. A. S. Compston, Douglas L. Arnold, Jeffrey Cohen, Alasdair Coles, Alastair Compston, Christian Confavreux, Edward Fox, Hans-Peter Hartung, Eva Havrdová, Krzysztof Selmaj, Howard L. Weiner, Hillel S. Panitch, David B. Clifford, Jack P. Antel, Frederik Barkhof, David R. Snydman, Leslie J. DeGroot, Douglas B. Cines, Ralph B. D’Agostino, Benjamin Greenberg, Jörg Krauss, Volker Limmroth, Clyde Markowitz, Robert T. Naismith, David Tabby, Norma Deri, Karyn Boundy, Simon Broadley, Michael Dreyer, Suzanne Hodgkinson, John O. King, Richard Macdonell, FAFRM RACP, Pamela McCombe, Mark Paine, Stephen W. Reddel, Raymond Schwartz, Steve Vucic, MD. Karl Vass, PhD Dominique Dive, Bénédicte Dubois, Christian Sindic, Dagoberto Callegaro, Maria Lúcia Brito Ferreira, Marcio Mena Barreto Martins, Charles Peter Tilbery, Charles Ayotte, Donald Brunet, Mark Freedman, François Grand’Maison, François Jacques, Marcelo Kremenchutzky, Anthony Traboulsee, MD Licia Antonelli, Vesna Brinar, Mario Habek, Spomenka Kiđemet Piskač, Zlatko Trkanjec, Anton Vladić, Ivana Kovářová, Ivan Rektor, Radomír Taláb, Marta Vachová, Thor Petersen, Mads Ravnborg, Per Soelberg Sørensen, Michel Clanet, Pierre Clavelou, de Sèze, Marc Debouverie, Gilles Edan, Catherine Lubetzki, Thibault Moreau, Patrick Vermersch, Karl Baum, Judith Haas, Bernhard Hemmer, Ulrich Herrlinger, Wolfgang Köhler, G. Ochs, Martin Stangel, Hayrettin Tumani, Péter Urbán, Uwe K. Zettl, Tjalf Ziemssen, Anat Achiron, Arnon Karni, Adi Vaknin Dembinsky, Antonio Bertolotto, Ruggero Capra, Giancarlo Comi, Luca Durelli, Angelo Ghezzi, Gianluigi Mancardi, Maria Giovanna Marrosu, Carlo Pozzilli, Noemí Santos Caballero, Claudia Venzor Mendoza, J. Villanueva, Raymond Hupperts, Erik van Munster, Wojciech Kozubski, Krzysztof Selmaj, Z Stelmasiak, Evgeniy Robertovich Barantsevich, DMedSc, Anna N. Belova, Alexey Boyко, Е. И. Гусев, N A Malkova, Semen V. Perfiliev, И Е Повереннова, A A Skoromets, I. D. Stolyarov, E Z Yakupov, И. А. Завалишин, Evica Dinčić, Jelena Drulović, Čongor Nadj, Gordana Tončev, Rafael Arroyo González, Guillermo Izquierdo‐Ayuso, Óscar Fernández, Xavier Montalbán, Jan Lycke, Anders Svenningsson, Tetyana Kobys, Tetyana Nehrych, Natalia P. Voloshyna, Gavin Giovannoni, David Rog, Neil Scolding, Basil Sharrack, Nuhad E. Abou Zeid, Mark Agius, Ann Bass, G. Kim Bigley, Roberto Bomprezzi, Aaron Boster, Christine Boutwell, Tiffany J. Braley, Mark Cascione, Gary Clauser, Joanna Cooper, Heidi Crayton, Jeffrey Dunn, Keith R. Edwards, Stanton B. Elias, Bradley K. Evans, Mark H. Fletcher, Corey C. Ford, E. Frohman, Suzanne Gazda, Thomas Giancarlo, Jeffrey Gitt, Steven A. Glyman, Andrew Goodman, Christopher Gottschalk, Malcolm Gottesman, Erica Grazioli, Mark Gudesblatt, Ajay S. Gupta, Joseph Herbert, William Honeycutt, Bruce L. Hughes, Samuel F. Hunter, Todd J. Janus, Adil Javed, Davis E. Jones, Burk Jubelt, Lily Jung Henson, Omar Khan, Mariko Kita, S Kirzinger, Stephen Krieger, Stanley J. Krolczyk, Christopher LaGanke, Enrico C. Lallana, Ellen Lathi, Neil Lava, Sharon Lynch, Bennett-Irving Machanic, Silva Markovic‐Plese, David H. Mattson, Daniel D. Mikol, Aaron Miller, Tamara Ann Miller, Alireza Minagar, Galen W. Mitchell, Harold L. Moses, Donald Negroski, Gabriel Pardo, Mary Ann Picone, Jana Lízrová Preiningerová, Kottil Rammohan, Claire Riley, Peter Riskind, Syed Rizvi, M.E.E. van Rossen, Ted L. Rothstein, Vernon Rowe, Walter Royal, John Schaeffer, Christopher A. Sheppard, Richard Shubin, Scott Silliman, B. Singer, Lee Stein, Brian Steingo, H. R. Sullivan, Timothy Thoits, Ben Thrower, Cary Twyman, Anand Vaishnav, Stephen G. Vincent, Timothy R. Vollmer, Stephen R. Waldman, Leslie P. Weiner, Jeanette Wendt, Dean M. Wingerchuk, Sibyl Wray, Daniel Wynn,
Objective: To characterize effects of alemtuzumab treatment on measures of disability improvement in patients with relapsing-remitting multiple sclerosis (RRMS) with inadequate response (≥1 relapse) to prior therapy. Methods: Comparison of Alemtuzumab and Rebif Efficacy in Multiple Sclerosis (CARE-MS) II, a 2-year randomized, rater-blinded, active-controlled, head-to-head, phase 3 trial, compared efficacy and safety of alemtuzumab 12 mg with subcutaneous interferon-β-1a (SC IFN-β-1a) 44 μg in patients with RRMS. ...
Tópico(s): Immunotherapy and Immune Responses
2016 - Lippincott Williams & Wilkins | Neurology
Produção Nacional
Revisado por pares
Marisa Jádna Silva Frederico, Allisson Jhonatan Gomes Castro, Danusa Menegaz, C. Murat, Camila Pires Mendes, Alessandra Mascarello, Ricardo José Nunes, Fátima Regina Mena Barreto Silva,
Glibenclamide is widely used and remains a cornerstone and an effective antihyperglycemic drug. After the casual discovery of its hypoglycemic potential, this compound was introduced for diabetes treatment. However, the long-term side effects reveal that glibenclamide should be replaced by new molecules able to maintain the health of β-cells, protecting them from hyperstimulation/hyperexcitability, hyperinsulinemia, functional failure and cell death. The aim of this review was to highlight the main ...
Tópico(s): Pancreatic function and diabetes
2016 - Bentham Science Publishers | Current Drug Targets
Produção Nacional
Revisado por pares
Fabiana Ourique, Maicon Roberto Kviecinski, Guilherme Zirbel, Luiza S. E. P. W. Castro, Allisson Jhonatan Gomes Castro, Fátima Regina Mena Barreto Silva, Jaime A. Valderrama, David Ríos, Julio Benites, Pedro Buc Calderón, Rozangela Curi Pedrosa,
The purpose of the study was to obtain further in vivo data of antitumor effects and mechanisms triggered by juglone and Q7 in combination with ascorbate. The study was done using Ehrlich ascites tumor-bearing mice. Treatments were intraperitoneal every 24 h for 9 days. Control group was treated with excipient. Previous tests selected the doses of juglone and Q7 plus ascorbate (1 and 100 mg/kg, respectively). Samples of ascitic fluid were collected to evaluate carbonyl proteins, GSH and activity ...
Tópico(s): Coenzyme Q10 studies and effects
2016 - Elsevier BV | Biochemical and Biophysical Research Communications
Produção Nacional
Revisado por pares
Allisson Jhonatan Gomes Castro, Luisa Helena Cazarolli, Francieli Kanumfre de Carvalho, Gabrielle da Luz, Delsi Altenhofen, Adair R.S. Santos, Moacir Geraldo Pizzolatti, Fátima Regina Mena Barreto Silva,
The effect of 3β-hidroxihop-22(29)ene (3-BHO) on insulin and glucagon-like peptide 1 (GLP-1) secretion as well as the mechanism of action of the compound in pancreatic islet on glucose homeostasis was investigated. The data from in vivo treatment show that 3-BHO significantly reduces the hyperglycemia by increasing the insulin and GLP-1 secretion, as well as by accumulating hepatic glycogen in hyperglycemic rats. In rat pancreatic β-cell, 3-BHO stimulates the glucose uptake, insulin vesicles translocation ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2015 - Elsevier BV | The Journal of Steroid Biochemistry and Molecular Biology
Produção Nacional
Revisado por pares
Catharina Nucci-Martins, Daniel F. Martins, Leandro F. Nascimento, Dalila Venzke, Aldo Sena de Oliveira, Marisa Jádna Silva Frederico, Fátima Regina Mena Barreto Silva, Inês Maria Costa Brighente, Moacir Geraldo Pizzolatti, Adair R.S. Santos,
The medicinal plant Pterodon pubescens Benth has been traditionally used for a long time to treat rheumatic diseases due to its anti-inflammatory and analgesic activities. The present study aims to evaluate the antinociceptive effect of ethanolic extract from P. pubescens fruits (EEPp) in a model of neuropathic pain in mice. The phytochemical analysis of EEPp was performed through GC–MS, HPLC and colorimetric analysis. The antinociceptive effects of EEPp (30–300 mg/kg, i.g.) were evaluated on mechanical ...
Tópico(s): Morinda citrifolia extract uses
2015 - Elsevier BV | Journal of Ethnopharmacology
Produção Nacional
Revisado por pares
João Bosko Formigas Relvas, Mariana Mena Barreto Bastos, André Augusto Franco Marques, Angela Delfina Bitencourt Garrido, Emílio Carlos Sponchiado Júnior,
Tópico(s): Occupational health in dentistry
2015 - Springer Nature | Clinical Oral Investigations
Produção Nacional
Revisado por pares
Allisson Jhonatan Gomes Castro, Marisa Jádna Silva Frederico, Luisa Helena Cazarolli, Camila Pires Mendes, Lizandra C. Bretanha, Éder C. Schmidt, Zenilda L. Bouzon, Verônica Aiceles de Medeiros Pinto, Cristiane da Fonte Ramos, Moacir Geraldo Pizzolatti, Fátima Regina Mena Barreto Silva,
The effect of in vivo treatment with ursolic acid (UA) on glycemia in hyperglycemic rats and its mechanism of action on muscle were studied. The UA effects on glycemia, glycogen, LDH, calcium and on insulin levels were evaluated after glucose tolerance curve. The β-cells were evaluated through the transmission electron microscopy. UA mechanism of action was studied on muscles through the glucose uptake with/without specific insulin signaling inhibitors. The nuclear effect of UA and the GLUT4 expression ...
Tópico(s): Phytochemicals and Antioxidant Activities
2014 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - General Subjects
Produção Nacional
Revisado por pares
Daiane Cattani, Vera Lúcia de Liz Oliveira Cavalli, Carla Elise Heinz Rieg, Juliana Tonietto Domingues, Tharine Dal‐Cim, Carla I. Tasca, Fátima Regina Mena Barreto Silva, Ariane Zamoner,
Previous studies demonstrate that glyphosate exposure is associated with oxidative damage and neurotoxicity. Therefore, the mechanism of glyphosate-induced neurotoxic effects needs to be determined. The aim of this study was to investigate whether Roundup(®) (a glyphosate-based herbicide) leads to neurotoxicity in hippocampus of immature rats following acute (30min) and chronic (pregnancy and lactation) pesticide exposure. Maternal exposure to pesticide was undertaken by treating dams orally with 1% ...
Tópico(s): Retinoids in leukemia and cellular processes
2014 - Elsevier BV | Toxicology
Produção Nacional
Revisado por pares
Samuel Vandresen-Filho, Patrícia Severino, Leandra C. Constantino, Wagner C. Martins, Simone Molz, Tharine Dal‐Cim, Daniela Bohn Bertoldo, Fátima Regina Mena Barreto Silva, Carla I. Tasca,
Tópico(s): Epilepsy research and treatment
2014 - Springer Science+Business Media | Neurotoxicity Research
Produção Nacional
Revisado por pares
Daiane Cattani, Paola Bez Goulart, Vera Lúcia de Liz Oliveira Cavalli, Elisa Cristiana Winkelmann Duarte, André Quincozes dos Santos, Paula Pierozan, Diogo O. Souza, Viviane Mara Woehl, Marilda da Cruz Fernandes, Fátima Regina Mena Barreto Silva, Carlos Alberto Gonçalves, Regina Pessoa‐Pureur, Ariane Zamoner,
Congenital hypothyroidism is associated with delay in cell migration and proliferation in brain tissue, impairment of synapse formation, misregulation of neurotransmitters, hypomyelination and mental retardation. However, the mechanisms underlying the neuropsychological deficits observed in congenital hypothyroidism are not completely understood. In the present study we proposed a mechanism by which hypothyroidism leads to hippocampal neurotoxicity. Congenital hypothyroidism induces c-Jun-N-terminal ...
Tópico(s): Neuroscience and Neuropharmacology Research
2013 - Elsevier BV | Molecular and Cellular Endocrinology
Produção Nacional
Revisado por pares
Vera Lúcia de Liz Oliveira Cavalli, Daiane Cattani, Carla Elise Heinz Rieg, Paula Pierozan, Leïla Zanatta, Eduardo Benedetti Parisotto, Danilo Wilhelm Filho, Fátima Regina Mena Barreto Silva, Regina Pessoa‐Pureur, Ariane Zamoner,
Glyphosate is the primary active constituent of the commercial pesticide Roundup. The present results show that acute Roundup exposure at low doses (36 ppm, 0.036 g/L) for 30 min induces oxidative stress and activates multiple stress-response pathways leading to Sertoli cell death in prepubertal rat testis. The pesticide increased intracellular Ca2+ concentration by opening L-type voltage-dependent Ca2+ channels as well as endoplasmic reticulum IP3 and ryanodine receptors, leading to Ca2+ overload ...
Tópico(s): Sperm and Testicular Function
2013 - Elsevier BV | Free Radical Biology and Medicine
Produção Nacional
Revisado por pares
Luisa Helena Cazarolli, Danielle Fontana Pereira, Virgínia Demarchi Kappel, Poliane Folador, Maria dos Santos Reis Bonorino Figueiredo, Moacir Geraldo Pizzolatti, Fátima Regina Mena Barreto Silva,
The aim of the study was to investigate the in vitro effect and the mechanism of action of kaempferitrin on glucose uptake in an insulin target (soleus muscle). A stimulatory effect of kaempferitrin on glucose uptake was observed when rat soleus muscle was incubated with 10, 100 and 1000 ηM of this flavonoid glycoside. The presence of specific insulin signaling inhibitors, such as wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K), RO318220, an inhibitor of protein kinase C (PKC), PD98059, ...
Tópico(s): Plant biochemistry and biosynthesis
2013 - Elsevier BV | European Journal of Pharmacology
Produção Nacional
Revisado por pares
Virgínia Demarchi Kappel, Luisa Helena Cazarolli, Danielle Fontana Pereira, Bárbara Graziela Postal, Fernanda Angélica Madoglio, Ziliani da Silva Buss, Flávio Henrique Reginatto, Fátima Regina Mena Barreto Silva,
The acute effect of crude extract, n-butanol and aqueous residual fractions of Musa x paradisiaca L., Musaceae, leaves on glycemia, serum insulin secretion and glycogen content in an in vivo approach was evaluated. In addition, the in vitro effect on disaccharidases activity and albumin glycation was studied. The crude extract and fractions, n-butanol and aqueous residual, reduced glycemia and increased liver glycogen content in hyperglycemic rats, inhibited maltase activity and the formation of ...
Tópico(s): Advanced Glycation End Products research
2013 - Springer Science+Business Media | Revista Brasileira de Farmacognosia
Produção Nacional
Revisado por pares
Allisson Jhonatan Gomes Castro, Marisa Jádna Silva Frederico, Luisa Helena Cazarolli, Lizandra C. Bretanha, Luciana de Carvalho Tavares, Ziliani da Silva Buss, Márcio Ferreira Dutra, Ariane Zamoner, Moacir Geraldo Pizzolatti, Fátima Regina Mena Barreto Silva,
The effect of betulinic acid on glycemia and its mechanism of action compared with 1,25(OH)2 vitamin D3 in rat muscle were investigated. Betulinic acid improved glycemia, induced insulin secretion and increased the glycogen content and glucose uptake in muscle tissue. Additionally, the integrity of both PI3K and the cytoskeleton is necessary for the stimulatory action of betulinic acid in glucose uptake. The genomic effect was apparent, since cycloheximide and PD98059 nullified the stimulatory effect ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2013 - Elsevier BV | The International Journal of Biochemistry & Cell Biology
Produção Nacional
Revisado por pares
Virgínia Demarchi Kappel, Luisa Helena Cazarolli, Danielle Fontana Pereira, Bárbara Graziela Postal, Ariane Zamoner, Flávio Henrique Reginatto, Fátima Regina Mena Barreto Silva,
Abstract Objectives The aim of this study was to investigate the in-vitro effect of rutin on glucose uptake in an insulin target (soleus muscle) and the mechanism of action involved. Methods Isolated soleus muscles from rats were treated with rutin (500 μm) with or without the following inhibitors; hydroxy-2-naphthalenylmethylphosphonic acid trisacetoxymethyl ester (HNMPA(AM)3), an insulin receptor tyrosine kinase activity inhibitor, wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
2013 - Oxford University Press | Journal of Pharmacy and Pharmacology
Produção Nacional
Revisado por pares
Ana Paula Zanatta, Leïla Zanatta, Renata Gonçalves, Ariane Zamoner, Fátima Regina Mena Barreto Silva,
There is increasing experimental evidence of the nongenomic action of thyroid hormones mediated by receptors located in the plasma membrane or inside cells. The aim of this work was to characterize the reverse T₃ (rT₃) action on calcium uptake and its involvement in immature rat Sertoli cell secretion. The results presented herein show that very low concentrations of rT₃ are able to increase calcium uptake after 1 min of exposure. The implication of T-type voltage-dependent calcium channels and chloride ...
Tópico(s): Hypothalamic control of reproductive hormones
2013 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Virgínia Demarchi Kappel, Marisa Jádna Silva Frederico, Bárbara Graziela Postal, Camila Pires Mendes, Luisa Helena Cazarolli, Fátima Regina Mena Barreto Silva,
Rutin is a flavonol glycoside with multiple biological activities and it has been demonstrated that rutin modulates glucose homeostasis. In pancreatic β-cell, an increase in intracellular calcium concentration triggers exocytosis and thus insulin secretion. The aim of the study reported herein was to investigate the effect of rutin associated intracellular pathways on Ca2+ uptake in isolated rat pancreatic islets. We focused on the acute effects of rutin on in vivo insulin secretion and the in vitro ...
Tópico(s): Diet, Metabolism, and Disease
2013 - Elsevier BV | European Journal of Pharmacology
Produção Nacional
Revisado por pares
Virgínia Demarchi Kappel, Leïla Zanatta, Bárbara Graziela Postal, Fátima Regina Mena Barreto Silva,
Rutin is a flavonoid with several pharmacological properties and it has been demonstrated that rutin can modulate glucose homeostasis. In skeletal muscle, an increase in intracellular calcium concentration may induce glucose transporter-4 (GLUT-4) translocation with consequent glucose uptake. The aim of this study was to investigate the effect of rutin and intracellular pathways on calcium uptake as well as the involvement of calcium in glucose uptake in skeletal muscle. The results show that rutin ...
Tópico(s): Calcium signaling and nucleotide metabolism
2013 - Elsevier BV | Archives of Biochemistry and Biophysics
Produção Nacional
Revisado por pares
Virgínia Demarchi Kappel, Danielle Fontana Pereira, Luisa Helena Cazarolli, Samantha Manes Guesser, Carlos H. Blum Da Silva, Eloir Paulo Schenkel, Flávio Henrique Reginatto, Fátima Regina Mena Barreto Silva,
In this study, the in vivo effect of the crude extract and n-butanol and aqueous residual fractions of Baccharis articulata (Lam.) Pers. on serum glucose levels, insulin secretion and liver and muscle glycogen content, as well as in vitro action on serum intestinal disaccharidase activity and albumin glycation were investigated. Oral administration of the extract and fractions reduced glycemia in hyperglycemic rats. Additionally, the n-butanol fraction, which has high flavonoids content, stimulated ...
Tópico(s): Advanced Glycation End Products research
2012 - Multidisciplinary Digital Publishing Institute | Molecules
Produção Nacional
Revisado por pares
Leïla Zanatta, Paola Bez Goulart, Renata Gonçalves, Paula Pierozan, Elisa Cristiana Winkelmann Duarte, Viviane Mara Woehl, Regina Pessoa‐Pureur, Fátima Regina Mena Barreto Silva, Ariane Zamoner,
The involvement of calcium-mediated signaling pathways in the mechanism of action of 1α,25-dihydroxyvitamin D3 (1,25D) is currently demonstrated. In this study we found that 1,25D induces nongenomic effects mediated by membrane vitamin D receptor (VDRm) by modulating intermediate filament (IF) phosphorylation and calcium uptake through L-type voltage-dependent calcium channels (L-VDCC) in cerebral cortex of 10 day-old rats. Results showed that the mechanism of action of 1,25D involves intra- and extracellular ...
Tópico(s): Stress Responses and Cortisol
2012 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Produção Nacional
Revisado por pares
Marisa Jádna Silva Frederico, Allisson Jhonatan Gomes Castro, Alessandra Mascarello, Camila Pires Mendes, Virginia Dermachi Kappel, Taisa Regina Stumpf, Paulo César Leal, Ricardo José Nunes, Rosendo Augusto Yunes, Fátima Regina Mena Barreto Silva,
In this study the in vivo and in vitro antidiabetic effects of four acylhydrazone derivatives were investigated in rats. The secretagogue action, oral glucose tolerance, insulinogenic index and mechanism of action of these acylhydrazones in relation to calcium uptake in pancreatic islets were studied. Also, the insulinomimetic effect on glycemia in diabetic rats was verified. Of the acylhydrazones studied, 1 and 4 were able to increase glucose tolerance in an acute time-course. A powerful secretagogue ...
Tópico(s): Metabolism, Diabetes, and Cancer
2012 - Bentham Science Publishers | Current Topics in Medicinal Chemistry