Inge Winter-van Rossum, Mark Weiser, Silvana Galderisi, Stefan Leucht, István Bitter, Birte Glenthøj, Alkomiet Hasan, Jurjen J. Luykx, Marina Kupchik, Georg Psota, Paola Rocca, Nikos Stefanis, Alexander Teitelbaum, Mor Bar Haim, Claudia Leucht, Georg Kemmler, Timo Schurr, Michael Davidson, René S. Kahn, W.W. Fleischhacker, René S. Kahn, Walter Wolfgang Fleischhacker, Michael Davidson, Inge Winter-van Rossum, Mark Weiser, Jurjen J. Luykx, Alkomiet Hasan, M.D. Mosescu, Silvana Galderisi, Marina Kupchik, Nikos Stefanis, Alexander Teitelbaum, Paola Rocca, Georg Psota, George Umoh, István Bitter, Lucho G Hranov, Alex Hofer, Joachim Cordes, Ramin Nilforooshan, Julio Bobes, Solveig Klebo Reitan, Manuel Morrens, Aurel Nireștean, John Geddes, Benedicto Crespo‐Facorro, Marcin Olajossy, Alessandro Rossi, Erik Johnsen, Csekey László, Adela Magdalena Ciobanu, Peter Haddad, Igor Oife, Miquel Bernardo, Rodicutza Stan, Marek Jarema, Dan Rujescu, Libor Ustohal, Neil Mayfield, Paola Dazzan, Avi Valevski, Jan Libiger, Richard Köhler, Pavel Mohr, Sofia Pappa, Petros Drosos, Thomas R. E. Barnes, Esther DeClercq, Elias Wagner, Paola Bucci, Armida Mucci, Yaacov Rabinowitz, Adam Adamopoulous, Benjamin Draiman, Cristiana Montemagni, Manfred Greslechner, Hannah Herlihy, Csilla Bolyós, Christian Schmidt-Kraepelin, Jessica True, Leticia Alvarez Garcia, Berit Walla, Bernhard Sabbe, Lucaks Emese, Sarah Mather, Nikodem Skoczeń, Serena Parnanzone, Jill Bjarke, Krisztina Karácsonyi, Steve Lankshear, Marina Garriga, Adam Wichniak, Heidi Baumbach, Timo Schurr, Leonie Willebrands, Lyliana Nasib, Cynthia Okhuijsen‐Pfeifer, Elianne Huijsman,
Schizophrenia is a severe psychiatric disorder with periods of remission and relapse. As discontinuation of antipsychotic medication is the most important reason for relapse, long-term maintenance treatment is key. Whether intramuscular long-acting (depot) antipsychotics are more efficacious than oral medication in preventing medication discontinuation is still unresolved. We aimed to compare time to all-cause discontinuation in patients randomly allocated to long-acting injectable (LAI) versus oral ...
Tópico(s): Pharmaceutical studies and practices
2023 - Elsevier BV | The Lancet Psychiatry
Han Boter, Joseph Peuskens, Jan Libiger, W. Wolfgang Fleischhacker, Michael Davidson, Silvana Galderisi, René S. Kahn,
Predefined response and remission criteria may hold more clinical relevance than mean scores on rating scales. We compared the effectiveness of low doses of haloperidol and regular doses of second generation antipsychotics (SGAs) on ≥ 50% response and remission. In an open randomized clinical trial in 14 countries, 498 unselected first-episode patients with schizophrenia were assigned to haloperidol (1–4 mg/d; n = 103), amisulpride (200–800 mg/d; n = 104), olanzapine (5–20 mg/d; n = 105), quetiapine (200–750 ...
Tópico(s): Bipolar Disorder and Treatment
2009 - Elsevier BV | Schizophrenia Research
Han Boter, Eske M. Derks, W. Wolfgang Fleischhacker, Michael Davidson, René S. Kahn,
Article Abstract Objective: Most randomized drug trials in schizophrenia exclude patients with comorbidities such as suicidality or substance use, which may limit the generalizability of the results. We aimed to evaluate the generalizability of the results of these trials in participants of a randomized clinical trial with broad inclusion criteria. Method: In 50 sites in 14 countries, 498 patients with first-episode psychosis (DSM-IV schizophrenia, schizoaffective disorder, or schizophreniform disorder) ...
Tópico(s): Bipolar Disorder and Treatment
2009 - Physicians Postgraduate Press, Inc. | The Journal of Clinical Psychiatry
Michael Fleischhacker, T. Beinert, Michael Ermitsch, DZEMILE SEFERI, Kurt Possinger, CLAUS ENGELMANN, Burkhard Jandrig,
Recently, in addition to the detection of circulating tumor cells in peripheral blood of patients with solid tumors, the presence of free circulating nucleic acids in the plasma and serum has also been described. We have focused on the possibility of isolating and amplifying intact extracellular, tumor-related mRNA from the plasma/serum of patients with lung cancer. For this purpose, we established several RT-PCR-based amplification systems for the detection of a panel of five different genes. The ...
Tópico(s): RNA Research and Splicing
2001 - Wiley | Annals of the New York Academy of Sciences
Giuseppe Lippi, Fay Betsou, Janne Cadamuro, Michael P. Cornes, Michael Fleischhacker, Palle Bach Nielsen Fruekilde, Michael Neumaier, Mads Nybo, Andrea Padoan, Mario Plebani, Laura Sciacovelli, Pieter Vermeersch, Alexander Meyer, Ana-Maria Šimundić,
Abstract The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE) was originally established in 2013, with the main aims of (i) promoting the importance of quality in the preanalytical phase of the testing process, (ii) establishing best practices and providing guidance for critical activities in the preanalytical phase, (iii) developing and disseminating European surveys for exploring practices concerning preanalytical issues, (iv) ...
Tópico(s): Meta-analysis and systematic reviews
2019 - De Gruyter | Clinical Chemistry and Laboratory Medicine (CCLM)
Christoph Kneip, Bernd Schmidt, Michael Fleischhacker, Anke Seegebarth, Jörn Lewin, Nadja Flemming, Stefanie Seemann, Thomas Schlegel, Christian Witt, Volker Liebenberg, Dimo Dietrich,
DNA methylation is an important epigenetic mechanism involved in fundamental biological processes such as development, imprinting, and carcino-genesis. For these reasons, DNA methylation represents a valuable source for cancer biomarkers. Methods for the sensitive and specific detection of methylated DNA are a prerequisite for the implementation of DNA biomarkers into clinical routine when early detection based on the analysis of body fluids is desired. Here, a novel technique is presented for the ...
Tópico(s): RNA modifications and cancer
2009 - Future Science Ltd | BioTechniques
B. Schmidt, Tim Carstensen, E Engel, Burkhard Jandrig, Christian Witt, Michael Fleischhacker,
The aim of this study was to determine whether nucleic acids are detectable in cell-free bronchial lavage supernatants, and whether it is possible to find alterations in this DNA and RNA of genes known to be present in lung tumour cells. DNA was isolated from cell-free lavage supernatants from 30 and RNA from 25 lung cancer patients. The DNA was examined for microsatellite alterations (MA) and the RNA analysed for the expression of seven tumour-associated genes. Intact DNA and mRNA could be isolated ...
Tópico(s): Genomics and Chromatin Dynamics
2003 - Elsevier BV | European Journal of Cancer
Monika Jung, Silke Klotzek, Michaela Lewandowski, Michael Fleischhacker, Klaus Jung,
Cell-free DNA in serum and plasma has been suggested to have diagnostic potential because associations between DNA concentrations and several disorders have been described (1). The concentration of cell-free DNA circulating in plasma and serum has been analyzed in several studies and used as an interchangeable index of the quantity of circulating DNA in blood (2)(3)(4). However, it is known that the DNA concentration in serum is ∼3- to 24-fold higher than in plasma (3)(4)(5)(6). Recently published articles ...
Tópico(s): Renal and related cancers
2003 - American Association for Clinical Chemistry | Clinical Chemistry
Sven de Vos, Sharon P. Wilczynski, Michael Fleischhacker, Phillip Koeffler,
Leiomyoma is the most common benign smooth muscle tumor. Although rare in other organs, it is frequent in the uterus, occurring in nearly 40% of women over 50 years of age. These benign tumors rarely undergo malignant transformation. The incidence of leiomyosarcomas in uterine leiomyomas is estimated to be between 0.13 and 0.29%. Little is known of the genetic abnormalities leading to this neoplasia. Loss of p53 function has been implicated in the pathogenesis of many human tumors. We evaluated ...
Tópico(s): Soft tissue tumors and treatment
1994 - Elsevier BV | Gynecologic Oncology
Michael Fleischhacker, Bernd Schmidt,
Tópico(s): Lung Cancer Treatments and Mutations
2008 - Nature Portfolio | Nature Medicine
Christoph Kirsch, Sabine Weickmann, Bernd Schmidt, Michael Fleischhacker,
Cell‐free DNA is of increasing interest in different fields, such as cancer research, prenatal diagnosis, and the diagnosis of nonmalignant diseases. The quantity of free‐circulating DNA in plasma, serum, and other body fluids is usually low and its isolation is still a challenge. Here we evaluate the application of the new commercially available NucleoSpin Plasma XS Kit for the isolation of cell‐free DNA from plasma, serum, and cell‐free bronchial lavage samples. The NucleoSpin columns proved superior ...
Tópico(s): Renal and related cancers
2008 - Wiley | Annals of the New York Academy of Sciences
Bernd Schmidt, Sabine Weickmann, Christian Witt, Michael Fleischhacker,
In several papers an increased quantity of cell‐free plasma DNA as well as the presence of long DNA fragments in cell‐free plasma and serum has been described. We isolated cell‐free DNA from plasma, serum, and bronchial lavage supernatants from 33 lung cancer patients and 27 patients with a benign lung disease. The DNA was amplified by real‐time PCR, and the quantity as well as the DNA integrity was determined. We did not find significant differences between the patient populations. Our results ...
Tópico(s): Genetic factors in colorectal cancer
2008 - Wiley | Annals of the New York Academy of Sciences
K. Weber, Reinhard Sohr, B. Schulz, Michael Fleischhacker, Markus Ruhnke,
Production of E,E-farnesol (FOH) and biofilm formation were studied under various conditions in 56 strains of eight Candida spp. FOH production differed significantly not only between Candida spp. but within Candida albicans strains as well. FOH concentrations and biofilm formation were the highest for C. albicans.
Tópico(s): Mycotoxins in Agriculture and Food
2008 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Julia Pasligh, Clarissa Radecke, Michael Fleischhacker, Markus Ruhnke,
Mixed infections caused by different Candida species are the rule rather than the exception. The discrimination between the two closely related species Candida albicans and Candida dubliniensis is not trivial. Therefore, there is a need for fast, reliable, and inexpensive methods with high specificity for the identification and differentiation of these two Candida species, which are frequently detected in the oral cavities of patients with a human immunodeficiency virus infection. We applied several ...
Tópico(s): Oral microbiology and periodontitis research
2010 - Elsevier BV | Journal of Microbiology Immunology and Infection
Bernd Schmidt, Volker Liebenberg, Dimo Dietrich, Thomas Schlegel, Christoph Kneip, Anke Seegebarth, Nadja Flemming, Stefanie Seemann, Jürgen Distler, Jörn Lewin, Reimo Tetzner, Sabine Weickmann, Ulrike Wille, Triantafillos Liloglou, Olaide Y. Raji, Martin Walshaw, Michael Fleischhacker, Christian Witt, John K. Field,
Abstract Background This study aimed to show that SHOX2 DNA methylation is a tumor marker in patients with suspected lung cancer by using bronchial fluid aspirated during bronchoscopy. Such a biomarker would be clinically valuable, especially when, following the first bronchoscopy, a final diagnosis cannot be established by histology or cytology. A test with a low false positive rate can reduce the need for further invasive and costly procedures and ensure early treatment. Methods Marker discovery ...
Tópico(s): Neonatal Respiratory Health Research
2010 - BioMed Central | BMC Cancer
Klaus Jung, Michael Fleischhacker, Anja Rabien,
Circulating cell-free DNA (cfDNA) has been suggested as a cancer biomarker. Several studies assessed the usefulness of quantitative and qualitative tumor-specific alterations of cfDNA, such as DNA strand integrity, frequency of mutations, abnormalities of microsatellites, and methylation of genes, as diagnostic, prognostic, and monitoring markers in cancer patients. Most of the papers that could be evaluated in this review resulted in a positive conclusion. However, methodical diversity without the ...
Tópico(s): Renal cell carcinoma treatment
2010 - Elsevier BV | Clinica Chimica Acta
Rubén Pı́o, Javier García, Leticia Corrales, Daniel Ajona, Michael Fleischhacker, María J. Pajares, Felipe Cardenal, Luis Seijó, Javier J. Zulueta, Ernest Nadal, Christian Witt, María D. Lozano, Bernd Schmidt, Luis M. Montuenga,
Abstract Background: Cytologic examination of specimens obtained from the respiratory tract is a lung cancer diagnostic procedure with high specificity but moderate sensitivity. The use of molecular biomarkers may enhance the sensitivity of cytologic examination in the detection of lung cancer. Methods: Complement factor H, a protein secreted by lung cancer cells, was quantified in a series of bronchoalveolar lavage supernatants from lung cancer patients and patients with nonmalignant respiratory ...
Tópico(s): Complement system in diseases
2010 - American Association for Cancer Research | Cancer Epidemiology Biomarkers & Prevention
Michael Fleischhacker, Julia Pasligh, Gary P. Moran, Markus Ruhnke,
We investigated the population structure of 208 Candida dubliniensis isolates obtained from 29 patients (25 human immunodeficiency virus [HIV] positive and 4 HIV negative) as part of a longitudinal study. The isolates were identified as C. dubliniensis by arbitrarily primed PCR (AP-PCR) and then genotyped using the Cd25 probe specific for C. dubliniensis. The majority of the isolates (55 of 58) were unique to individual patients, and more than one genotype was recovered from 15 of 29 patients. A total ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2010 - American Society for Microbiology | Journal of Clinical Microbiology
Michael Fleischhacker, Bernd Schmidt,
Recent results indicate that not only intact circulating tumor cells but also extracellular, free-circulating nucleic acids containing tumor-associated genetic alterations can be detected in peripheral blood of cancer patients. The development of sen
Tópico(s): Advanced biosensing and bioanalysis techniques
2010 - IOS Press | Cancer Biomarkers
Michael Fleischhacker, Clarissa Radecke, B. Schulz, Markus Ruhnke,
Tópico(s): Mycotoxins in Agriculture and Food
2007 - Springer Science+Business Media | European Journal of Clinical Microbiology & Infectious Diseases
Anne Whitworth, H. Oberbauer, WW Fleischhacker, Otto‐Michael Lesch, H. Walter, A. Nimmerrichter, T Platz, Felix Fischer, A. Potgieter,
SummaryBackground About 50% of alcoholic patients relapse within 3 months of treatment. Previous studies have suggested that acamprosate may help to prevent such relapse. The aim of our study was to assess the efficacy and safety of long-term acamprosate treatment in alcohol dependence.Methods In this multicentre, double-blind, placebo-controlled study, we recruited 455 patients, aged 18-65 years, with chronic or episodic alcohol dependence. Patients were randomly allocated treatment with acamprosate ( ...
Tópico(s): Alcohol Consumption and Health Effects
1996 - Elsevier BV | The Lancet
Norbert Bruhn, T. Beinert, C. Oehm, Burkhard Jandrig, Iver Petersen, Xiaowei CHEN, K. Possinger, Michael Fleischhacker,
A bstract : In this paper, we show that the same panel of three microsatellite markers is useful for the detection of alterations in the DNA of tumor cells and plasma from patients diagnosed with SCLC and NSCLC. In 31% of the SCLC patients, we detected a microsatellite alteration(s) or LOH in at least one locus. In the group of patients diagnosed with NSCLC, a microsatellite alteration or LOH was detected in at least one locus in 33% of the patients. In all but 2 patients, the identical alteration ...
Tópico(s): Cholangiocarcinoma and Gallbladder Cancer Studies
2000 - Wiley | Annals of the New York Academy of Sciences
T. Beinert, D. Binder, C. Oehm, Sabine Ziemer, Friedrich Priem, M. Stuschke, Michael Schweigert, Gerda Siebert, H.‐G. Mergenthaler, P. Schmid, Michael Fleischhacker, K. Possinger,
Tópico(s): Angiogenesis and VEGF in Cancer
2000 - Springer Science+Business Media | Journal of Cancer Research and Clinical Oncology
Michael Fleischhacker, Dimo Dietrich, Volker Liebenberg, John K. Field, Bernd Schmidt,
It is now widely accepted that cancer is caused by complex interactions between genetic and epigenetic factors and the environment. Only in the last 20 years, DNA methylation has been recognized as an epigenetic mechanism, which plays a major role during the development and progression of cancers. Accordingly, DNA methylation profiling provides a useful source for biomarkers in distinct clinical questions; for example, risk stratification, diagnosis, staging, prognosis and therapy-response prediction. ...
Tópico(s): Cancer-related molecular mechanisms research
2013 - Taylor & Francis | Expert Review of Respiratory Medicine
Michael Fleischhacker, Bernd Schmidt, Sabine Weickmann, Debora M.I. Fersching, Gloria Leszinski, Barbara Siegele, Oliver Stötzer, Dorothea Nagel, Stefan Holdenrieder,
Tópico(s): Renal and related cancers
2011 - Elsevier BV | Clinica Chimica Acta
Christoph Kneip, Bernd Schmidt, Anke Seegebarth, Sabine Weickmann, Michael Fleischhacker, Volker Liebenberg, John K. Field, Dimo Dietrich,
IntroductionRecently, analysis of DNA methylation of the SHOX2 locus was shown to reliably identify lung cancer in bronchial aspirates of patients with disease. As a plasma-based assay would expand the possible applications of the SHOX2 biomarker, this study aimed to develop a modified SHOX2 assay for use in a blood-based test and to analyze the performance of this optimized SHOX2 methylation assay in plasma.MethodsQuantitative real-time polymerase chain reaction was used to analyze DNA methylation ...
Tópico(s): RNA modifications and cancer
2011 - Elsevier BV | Journal of Thoracic Oncology
Katja Schneider, Dimo Dietrich, Michael Fleischhacker, Gunda Leschber, Johannes Merk, Frank Schäper, Henk Stapert, Erik R Vossenaar, Sabine Weickmann, Volker Liebenberg, Christoph Kneip, Anke Seegebarth, Fikret Erdogan, Gudrun Rappold, Bernd Schmidt,
Abstract Background DNA methylation in the SHOX2 locus was previously used to reliably detect lung cancer in a group of critical controls, including 'cytologically negative' samples with no visible tumor cell content, at a high specificity based on the analysis of bronchial lavage samples. This study aimed to investigate, if the methylation correlates with SHOX2 gene expression and/or copy number alterations. An amplification of the SHOX2 gene locus together with the observed tumor-specific hypermethylation ...
Tópico(s): RNA modifications and cancer
2011 - BioMed Central | BMC Cancer
Michael Fleischhacker, Stefan Schulz, Korinna Jöhrens, Marie von Lilienfeld‐Toal, Thomas Held, Ellen Fietze, C. Schewe, Iver Petersen, Markus Ruhnke,
Tópico(s): Bacterial Identification and Susceptibility Testing
2011 - Elsevier BV | Clinical Microbiology and Infection
Michael Fleischhacker, B. Schmidt,
It has been known for decades that it is possible to detect small amounts of extracellular nucleic acids in plasma and serum of healthy and diseased human beings. The unequivocal proof that part of these circulating nucleic acids (CNAs) is of tumor origin, initiated a surge of studies which confirmed and extended the original observations. In the past few years many experiments showed that tumor-associated alterations can be detected at the DNA and RNA level. At the DNA level the detection of point ...
Tópico(s): RNA modifications and cancer
2006 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer
A few years after the first description of free-circulating DNA in plasma and serum, the detection of tumor-associated overexpressed mRNA in plasma was also reported. This observation has been confirmed and it seems to be clear that the presence of free-circulating RNA is a ubiquitous phenomenon. In this short review I will discuss some basic aspects of the release mechanisms for the RNA, its biological meaning, and clinical value.
Tópico(s): Renal and related cancers
2006 - Wiley | Annals of the New York Academy of Sciences