J L Preud'homme, Michel Klein, S Labaume, M Séligmann,
Abstract Twenty‐five to 30 % of blood lymphocytes from a myeloma patient with an IgGl( k ) protein with antibody activity to horse α 2 ‐macroglobulin (α2M) reacted with purified IgG from a rabbit antiserum to the idiotypic determinants of this protein. A small proportion of these idiotype‐bearing lymphocytes were IgG‐bearing B cells, but most of them were T cells. Several experiments demonstrated the actual synthesis of the idiotypic structures carried by the T lymphocytes. All idiotype‐bearing lymphocytes ...
Tópico(s): Immune Cell Function and Interaction
1977 - Wiley | European Journal of Immunology
Pièrre Aucouturier, Marc Bauwens, Ahmed Amine Khamlichi, Luc Denoroy, S. Spinelli, Guy Touchard, J L Preud'homme, Michel Cogné,
Abstract Monoclonal free L chains secreted in immunoproliferative disorders are frequently involved in renal complications, including a specific proximal tubule impairment, the Fanconi's syndrome, which is generally featured by intracellular crystallization of L chain-related material. In a patient with myeloma-associated Fanconi's syndrome, hexagonal crystals (most surrounded by smooth membranes) were found in kidney proximal tubular cells and bone marrow plasma cells and phagocytes. The sequence ...
Tópico(s): Drug Transport and Resistance Mechanisms
1993 - American Association of Immunologists | The Journal of Immunology
Anna Rocca, Ahmed Amine Khamlichi, Pièrre Aucouturier, L Guillot Noel, Luc Denoroy, J-L PREUD'HOMME, Michel Cogné,
Although structural abnormalities of monoclonal immunoglobulin light chains (LC) are suspected to play a determinant role in non-amyloid light chain deposition disease (LCDD), this condition is as yet poorly documented at the molecular level, since only three sequences have been reported to date. In a case of myeloma-associated LCDD, the patient's urine contained an unglycosylated kappa Bence Jones protein made up of dimers and monomers with an apparent molecular mass of 25,000 which was assigned ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1993 - Oxford University Press | Clinical & Experimental Immunology
Isabelle Leduc, J-L PREUD'HOMME, Michel Cogné,
SUMMARY The mb-1 gene encodes a protein associated with membrane-bound immunoglobulins (mlg) and it has been suggested that it may play a role in signal iransduction. Using a murine probe, we cloned a human complementary DNA homologous to the murine mb-1 sequence. Its complete sequence is in full agreement with a recently published human cDNA sequence but differs from a previously reported partial sequence. We studied mb-1 expression in human cells at various maturation stages. Large amounts of a ...
Tópico(s): Cell Adhesion Molecules Research
1992 - Oxford University Press | Clinical & Experimental Immunology
Marie‐Odile Jauberteau, N. Gualde, Jean‐Louis Preud'homme, Michel Rigaud, R. Gil, J M Vallat, Nicole Baumann,
SUMMARY Small amounts of oligoclonal immunoglobulins were detected by Western blotting in the serum from a patient with motor neuron syndrome. The prominent one. a monoclonal IgMλ, reacted strongly with the gangliosides GM1 and GDlb and more weakly with asialo GM1, as shown by immunoenzymatic staining of thin-layer chromatograms of gangliosides, ELISA on purified glycolipid coats and immunoadsorption with purified GM1. Affinity-chromatography with purified GMI resulted in the purification of monoclonal ...
Tópico(s): Trypanosoma species research and implications
1990 - Oxford University Press | Clinical & Experimental Immunology
Catherine Decourt, Anna Rocca, Frank Bridoux, François Vrtovsnik, Jean‐Louis Preud'homme, Michel Cogné, Guy Touchard,
We have designed an in vivo model in which murine hybridoma cell clones producing human Ig light chains (LC) are administred to mice. Depending on which monoclonal LC is expressed, this model mimicks either cast myeloma nephropathy or the pathological condition defined as myeloma-associated Fanconi’s syndrome (FS) with LC crystallization. Morphological alterations of the kidney cells are thus obtained in mice. All studied LC are closely related human monoclonal VκI proteins, which differ by a limited ...
Tópico(s): Microtubule and mitosis dynamics
1999 - Elsevier BV | Blood
Ahmed Amine Khamlichi, Pièrre Aucouturier, Jean‐Louis Preud'homme, Michel Cogné,
The sequences of two immunoglobulin gamma 1 heavy chains involved in the formation of non-amyloid tissue deposits were determined in two patients (RIC and THR) affected with plasma cell monoclonal proliferation and heavy-chain-deposition disease. The proliferating plasma cells of patients RIC and THR synthesized truncated gamma 1 chains of 45 kDa and 24 kDa, respectively, carrying internal deletions of the first constant (CH1) domain (RIC) or of the CH1, hinge and CH2 domains(THR). The shortened gamma ...
Tópico(s): Amyloidosis: Diagnosis, Treatment, Outcomes
1995 - Wiley | European Journal of Biochemistry
Ahmed Amine Khamlichi, Anna Rocca, Guy Touchard, Pièrre Aucouturier, JL Preud'homme, Michel Cogné,
Light chain deposition disease (LCDD) results from a propensity of some human monoclonal L chains to form tissue deposits. We designed an experimental model for in vivo expression of human kappa L chain sequences in mice and compared a somatically mutated LCDD chain with a closely related control kappa chain, both encoded by the unique V kappa IV gene. Mice secreting the LCDD chain but not those producing the control chain showed deposits with a distribution similar to that observed in patients. ...
Tópico(s): Amyloidosis: Diagnosis, Treatment, Outcomes
1995 - Elsevier BV | Blood
Ahmed Amine Khamlichi, Pièrre Aucouturier, Jean‐Louis Preud'homme, Michel Cogné,
Tópico(s): Genetic Neurodegenerative Diseases
1995 - Wiley | European Journal of Biochemistry
Viviane Pierrard, Graciela López Rosson, K. Borremans, J. Lemaire, Jeroen Maes, Sabrina Bonnewijn, E. Van Ransbeeck, Eddy Neefs, M. Cyamukungu, S. Benck, Luc Bonnet, С. В. Борисов, J. Cabrera, G. Grégoire, Christophe Semaille, Glenn Creve, Jurgen De Saedeleer, B. Desoete, F. Preud’homme, Michel Anciaux, A. Helderweirt, K. Litefti, N. Brun, D. Pauwels, Cyril Quevrin, D. Moreau, Risto Punkkinen, Э. Валтонен, W. Hajdas, P. Nieminen,
Tópico(s): Astro and Planetary Science
2014 - Springer Science+Business Media | Space Science Reviews
Murielle Girard, Z. Ayed, Pierre‐Marie Preux, Bernard Bouteille, Jean‐Louis Preud'homme, Michel Dumas, Marie‐Odile Jauberteau,
In stage II human african trypanosomiasis (HAT), which is characterized by central nervous system (CNS) involvement, neurones and oligodendrocytes might be targets of dysimmune processes. Nitric oxide (NO) production by peripheral macrophages is documented in HAT. We studied the production of NO by murine astrocytes and microglia cocultured with Trypanosoma brucei (T. b.) brucei AnTat 1.9. Purified astrocytes or microglia from mouse brains were cocultured with T. b. brucei, and in some instances with ...
Tópico(s): Helminth infection and control
2000 - Wiley | Parasite Immunology
Isabelle Brindel, Jean‐Louis Preud'homme, Jean‐Michel Vallat, Didier Vincent, Jean-Luc Vasquez, Marie‐Odile Jauberteau,
A possibly pathogenic serum monoclonal IgMλ from a patient with chronic relapsing polyneuropathy was shown to react with the disialosyl-lactosyl residue (NeuAcα2–8NeuAcα2–3)-Ga1β1-4Glc expressed by GD1b, GT1b, GQ1b, GD2 and GD3. A part of this epitope in terminal position in GM3, GD1a and LM1 was also recognized by the IgMλ.
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1994 - Elsevier BV | Neuroscience Letters
T Defêchereux, C Degauque, Etienne Hamoir, G Lamberty, Laurence Preud'Homme, Michel Meurisse,
The accurate assessment of tumour size is an important consideration during preoperative evaluation of adrenal tumors, particularly incidentaloma; however the "size criteria" is still a controversial topic in some respects: size is a bad indicator of malignancy, there is still a confusion in the "grey zone" for tumors between 3 and 6 cm, and no universal consensus on the exact cut-off value for resection has been agreed. Nowadays it is clearly accepted that the "size criteria" alone is extremely limited ...
Tópico(s): Renal cell carcinoma treatment
2002 - Elsevier BV | Annales de Chirurgie
Jean–Claude Rambaud, A Galian, F Danon, Jean‐Louis Preud'homme, Per Brandtzæg, Michel Wassef, Michel Le Carrer, M Méhaut, O Voinchet, R Pérol, A. Chapman,
Tópico(s): Chronic Lymphocytic Leukemia Research
1983 - Wiley | Cancer
Jean–Claude Rambaud, A Galian, F Danon, Jean‐Louis Preud'homme, Per Brandtzæg, Michel Wassef, Michel Le Carrer, M Méhaut, O Voinchet, R Pérol, A. Chapman,
Two cases of alpha-chain disease (alpha-CD) without detectable amounts of alpha-CD protein in serum are reported. Both patients presented with the usual epidemiologic, clinical and pathologic features of alpha-CD, including a predominantly plasmacytic infiltration of the whole small intestine, alpha-CD Protein was found in the jejunal fluid in one case but not in the other, the latter presenting as a "nonsecretory" form of alpha-CD. In both cases, immunofluorescence study of the small bowel mucosa ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1983 - Wiley | Cancer
Ahmed Amine Khamlichi, Pièrre Aucouturier, Christine Silvain, Marc Bauwens, Guy Touchard, J-L PREUD'HOMME, François Nau, Michel Cogné,
SUMMARY Previous data suggest that structural abnormalities of immunoglobulin light chains may be responsible for non-amyloid light chain deposition disease (LCDD). We report on the complete primary sequence deduced from complementary (c)DNA analysis of a normal-sized κ chain in a case of myeloma-associated LCDD. The patient's urine contained a κ type Bence-Jones protein made of monomers and dimers of an unglycosylated κ chain. The bone marrow myeloma cells contained intracellular κ and γ chains by ...
Tópico(s): Glycosylation and Glycoproteins Research
1992 - Oxford University Press | Clinical & Experimental Immunology
Anna Rocca, Ahmed Amine Khamlichi, Guy Touchard, B. Mougenot, Pierre Ronco, Luc Denoroy, Sophie Déret, Jean‐Louis Preud'homme, Pièrre Aucouturier, Michel Cogné,
Abstract Certain monoclonal Ig light chains (LC) are responsible for marked disturbances of proximal tubule cell functions (Fanconi's syndrome, FS). In patients with FS, intracellular crystal-like inclusions containing LC determinants are commonly found in plasma cells, macrophages, and renal tubular cells. In an attempt at understanding the pathogenesis of myeloma-associated FS, we recently determined the first complete primary sequence of a kappa-LC (CHEB) responsible for the disease. We now report ...
Tópico(s): Microtubule and mitosis dynamics
1995 - American Association of Immunologists | The Journal of Immunology
Samir Mounir, P Guglielmi, J L Preud'homme, François Nau, Michel Cogné,
The Burkitt's lymphoma cell lines JBL2 and Ly91 contain intracytoplasmic mu chains without L chain by immunofluorescence. These mu chains and the corresponding cDNA are abnormally short. Detailed analysis of the productive H chain alleles showed that the cell lines JBL2 and Ly91 use a VHIV and a VHIII subgroup gene, respectively. In both cell lines, the 3' part of the V exon was markedly abnormal and contained several stop codons. Point mutations affected the JH6 donor splice site in the JBL2 gene, ...
Tópico(s): Galectins and Cancer Biology
1990 - American Association of Immunologists | The Journal of Immunology
Pièrre Aucouturier, Ahmed Amine Khamlichi, J L Preud'homme, Marc Bauwens, Guy Touchard, Michel Cogné,
The primary structure of three amyloid precursor light chains was deduced from the sequence of complementary DNA (cDNA) from bone marrow cells from patients affected with classical lambda (patient Air) or kappa (patient Arn) amyloidosis and from a patient (Aub) in whom lambda amyloid deposits were unusual by their perimembranous location in the kidney glomerulus. All three RNAs were of normal size, as estimated by Northern blotting, and encoded normal-sized light chains. The deduced light-chain sequence ...
Tópico(s): Alzheimer's disease research and treatments
1992 - Portland Press | Biochemical Journal
Christine Silvain, Pièrre Aucouturier, Isabelle Leduc, Edith Mihaesco, Jean‐Louis Preud' Homme, Michel Cogné,
Abstract A human monoclonal IgA1‐IgA2λ. hybrid molecule was detected in a myeloma patient homozygous for the A2m(1) allotype during a systematic study of monoclonal IgA with subclass‐specific monoclonal antibodies (mAb) and the lectin jacalin. This monoclonal immunoglobulin (GAU) reacted with both, although not with all, anti‐α1 and anti‐α2 mAb. Its heavy (H) chain contained an al hinge region as shown by jacalin reactivity, the presence of disulfide‐linked H and light chains in spite of its belonging ...
Tópico(s): Chronic Lymphocytic Leukemia Research
1993 - Wiley | European Journal of Immunology
Michel Cogné, Jean‐Louis Preud'homme, Marc Bauwens, Guy Touchard, Pièrre Aucouturier,
That structural abnormalities may be responsible for nonamyloid immunoglobulin (Ig) light chain deposition disease (LCDD) is suggested by previous results of Ig biosynthesis studies, but this hypothesis was not documented at the molecular level. We report on the first complete primary sequence deduced from cDNA analysis of a kappa light chain responsible for LCDD associated with an apparently nonsecretory myeloma. Bone marrow myeloma cells contained intracellular kappa chains and no heavy chains by ...
Tópico(s): Amyloidosis: Diagnosis, Treatment, Outcomes
1991 - American Society for Clinical Investigation | Journal of Clinical Investigation
Michel Cogné, J L Preud'homme,
We studied a case of nonsecretory alpha-chain disease. The proliferating plasma cells contained a short transcript coding for a truncated membrane-form alpha 1-chain. The productive alpha-gene bore several noncontiguous deletions affecting the VHDJH and CH1 regions. Two deletions were accompanied with peculiar insertions containing duplications. The first insertion contained an acceptor splice site and was present in part in the mature transcript, thus coding for an abnormal aminoterminal peptide. ...
Tópico(s): Genomics and Chromatin Dynamics
1990 - American Association of Immunologists | The Journal of Immunology
Michel Cogné, Jean‐Louis Preud'homme, P Guglielmi,
Tópico(s): Diabetes and associated disorders
1989 - Elsevier BV | Research in Immunology
Catherine Decourt, Guy Touchard, Jean-Louis Preud'homme, Rubén Vidal, Hélène Beaufils, Marie‐Claude Diemert, Michel Cogné,
We herein report on the first two primary sequences (BOU and RAC) of monoclonal light chains of the λ type responsible for nonamyloid λ light chain deposition disease. Both patients were affected with severe forms of myeloma complicated with renal failure. The pathological presentation typically featured Congo red-negative deposits along tubular basement membranes but differed somewhat from the typical “Randall-type” κ light chain deposition disease: they lacked the prominent glomerulosclerosis pattern ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
1998 - Elsevier BV | American Journal Of Pathology
Philippe Couratier, Feng-Hua Yi, Jean-Louis Preud'homme, Pierre Clavelou, Aisha White, Philippe Sindou, Jean-Michel Vallat, Marie‐Odile Jauberteau,
Anti-neurofilament (NF) autoantibodies were searched for by enzyme-linked immunosorbent assays (ELISA) in the serum from 85 sporadic amyotrophic lateral sclerosis (ALS) patients, 98 healthy controls and 79 patients with unrelated immunological diseases (Guillain–Barré syndrome, myasthenia gravis and multiple sclerosis). ELISA cutoff value was determined as mean control levels +2 SD and it corresponded to a specificity of 94%. Such high level antibodies were detected in 24.7% of ALS patients contrasting ...
Tópico(s): Peripheral Neuropathies and Disorders
1998 - Elsevier BV | Journal of the Neurological Sciences
V Roussel, Fan Yi, Marie‐Odile Jauberteau, C Couderq, Corinne Lacombe, V Michelet, R. Gil, Philippe Couratier, Jean‐Michel Vallat, J L Preud'homme,
The prevalence of serum anti-phospholipid antibodies (aPL) was evaluated in multiple sclerosis (MS) patients to search for a possible association with a distinct form of the disease. Anti-cardiolipin antibodies (Ab) (aCL) and anti-beta 2 glycoprotein I Ab (abeta2GPI) were measured together with antinuclear Ab (ANA), anti-double-stranded DNA Ab (anti-ds DNA) and anti-myelin Ab in 89 patients. Twenty-nine (32.6%) patients had serum aPL, 19xaCL (15 of the IgG and four of the IgM isotype), 14 abeta2GPI (two IgG ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2000 - Elsevier BV | Journal of Autoimmunity
Claude Matuchansky, Guy Touchard, P Babin, Marc Lemaire, Michel Cogné, Jean‐Louis Preud'homme,
Tópico(s): Whipple's Disease and Interleukins
1988 - Elsevier BV | Gastroenterology
I. Brindel, J L Preud'homme, Jean‐Jacques Diaz, C. Giraud, Jean-Michel Vallat, Marie‐Odile Jauberteau,
Serum monoclonal IgMλ from a patient with axonal neuropathy reacted with axonal, nuclear, cytoplasmic and cell surface components by immunofluorescence. Experiments performed with Fab fragments of the monoclonal IgM proved that this reactivity was clearly due to actual antibody activity. Further study by Western blotting and ELISA showed that the IgMλ reacted with the 200 kDa neurofilament protein, H1 and H2b histones and L14, L24 and L7 ribosomal proteins. This reactivity was abolished after adsorption ...
Tópico(s): Skin and Cellular Biology Research
1995 - Elsevier BV | Journal of Autoimmunity
Jean‐Louis Preud'homme, Pièrre Aucouturier, Guy Touchard, Ahmed Amine Khamlichi, Anna Rocca, Luc Denoroy, Michel Cogné,
Monoclonal immunoglobulin deposition disease, a complication of overtly malignant or apparently benign immunoproliferative disorders, is a severe disease featuring tissue deposition of monoclonal light, light and heavy, or heavy chains. A number of converging arguments strongly suggest a direct pathogenetic role of structural abnormalities or peculiarities of variables regions of light and/or heavy chains (associated with deletions in the constant region for the heavy chains). Recent structural data ...
Tópico(s): Blood properties and coagulation
1994 - Elsevier BV | International Journal of Immunopharmacology
Nathalie Pineau, Jean-Christophe Brugier, Jean-Philippe Breux, B Becq-Giraudon, Jean-Michel Descamps, Pièrre Aucouturier, Jean‐Louis Preud'homme,
The proliferative response of peripheral blood mononuclear cells stimulated by jacalin was investigated in 157 samples from 104 HIV-infected patients at various clinical stages of disease. Jacalin proliferative response correlated with the absolute numbers and percentages of CD4+ lymphocytes and with the percentages of Leu 8 lymphocytes. It correlated negatively with the percentages of CD8+ and CD38+ lymphocytes. It was depressed during acute HIV infection and at advanced stages., The response in ...
Tópico(s): Glycosylation and Glycoproteins Research
1989 - Lippincott Williams & Wilkins | AIDS