Moriya Ohkuma, Shinichiro Muraoka, Takeshi Tanimoto, Michihiko Fujii, Akinori Ohta, Masamichi Takagi,
Previously, we characterized three genes and presented evidence for an n-alkane-inducible cytochrome P450 (P450alk) multigene family in an n-alkane-assimilating and diploid-type yeast, Candida maltosa. In the present report, we isolated and characterized additional members of this gene family, including a total of thirteen P450alk-related sequences (eight genes and five of their alleles). Two sets, each consisting of two genes, were tandemly arranged in the genome. A gene replacement experiment showed ...
Tópico(s): Biofuel production and bioconversion
1995 - Mary Ann Liebert, Inc. | DNA and Cell Biology
Shinsuke Ohta, Michihiko Yatazawa,
The effect of light on nicotine production in cultured tobacco callus tissues was investi-gated. Illumination strikingly inhibited the nicotine production even though the growth of the tissues was slightly stimulated by the light. The inhibitory effect of light increased as the intensity and the length of the illumination increased. No decisive difference in nicotine production was observed between the effects of blue light and red light. The depression of nicotine production by light was restored ...
Tópico(s): Soybean genetics and cultivation
1978 - Oxford University Press | Agricultural and Biological Chemistry
Saira Hameed, Koji Ikegami, Eiji Sugiyama, Shoko Matsushita, Yoshishige Kimura, Takahiro Hayasaka, Yuki Sugiura, Noritaka Masaki, Michihiko Waki, Isao Ohta, Md Amir Hossen, Mitsutoshi Setou,
A protocol for the direct analysis of the phospholipid composition in the whole body of adult soil nematode, Caenorhabditis elegans (C. elegans), was developed, which combined freeze-cracking of the exoskeletal cuticle and matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Biomolecules in the m/z range from 700 to 900 were more effectively detected in the freeze-cracked than from simple frozen adult nematode bodies. Different distribution of biomolecules was observed ...
Tópico(s): Genetics, Aging, and Longevity in Model Organisms
2015 - Springer Science+Business Media | Analytical and Bioanalytical Chemistry
Y. Saito, T. Yamagami, Y. Matsuzaka, Michiyoshi Namiki, Shigeo Ohta, Michihiko Toriumi, Rikio Yokota, F. Makino, Takuma Matsumoto, H. Hirosawa,
Abstract The balloon group at the Institute of Space and Astronautical Science (ISAS) has studied to develop high altitude balloons that could reach an altitude of more than 40 km with a light payload in order to meet the requirements of observations at extremely high altitudes. In 1999, we developed a ultra thin balloon film with a thickness of 3.4 μm. It is synthesized with a new catalyst, metallocene, and has a ultimate strength of ∼650 kg/cm 2 at −80°C. Using the film, we made the first balloon ...
Tópico(s): Spacecraft and Cryogenic Technologies
2002 - Elsevier BV | Advances in Space Research
Shinsuke Ohta, Michihiko Yatazawa,
Callus tissues were induced from stem and root segments of Rauwolfa serpentina. Growth and alkaloid production of the callus tissues were examined under various culture conditions. The growth was strikingly promoted in the presence of 2, 4-D (0.5_??_1ppm), kinetin (0.2_??_0.5ppm) and yeast extract (0.1_??_0.2%). At favourable conditions, the growth value in 4 weeks' culture was ca. 40 (F. W.), and ca. 25 (D. W.) for stem callus tissues, and ca. 15 (F. W.), and ca. 8 (D. W.) for root callus tissues. Stem ...
Tópico(s): Plant tissue culture and regeneration
1979 - Oxford University Press | Agricultural and Biological Chemistry
Y. Matsuzaka, T. Yamagami, Michiyoshi Namiki, Michihiko Toriumi, Shigeo Ohta, Fumiyoshi Makino, H. Hirosawa,
The development of high altitude balloons will enrich many areas of scientific observation. A high altitude balloon fabricated using improved technology with thin Winzen polyethylene films has been flown in the upper atmosphere. A light weight balloon carrying small instruments can reach an altitude higher than 40 km. In contrast to sounding rockets, the balloon-borne experiment provides a unique opportunity to stay at a high altitude for more than a few hours at low cost. We have developed methods ...
Tópico(s): Underwater Vehicles and Communication Systems
2000 - Elsevier BV | Advances in Space Research
Shinsuke Ohta, Michihiko Yatazawa,
Tópico(s): Fungal and yeast genetics research
1980 - Elsevier BV | Biochemie und Physiologie der Pflanzen
Shao-Pin Chiu, Michihiko Yamanouchi, Tatsuro Oyamada, Hiromichi Ohta, Juhn‐Jong Lin,
Epitaxial $\mathrm{L}{\mathrm{a}}_{2/3}\mathrm{S}{\mathrm{r}}_{1/3}\mathrm{Mn}{\mathrm{O}}_{3}$ (LSMO) films have been grown on $\mathrm{SrTi}{\mathrm{O}}_{3}$ (001) substrates via pulsed laser deposition. In a 22-nm-thick LSMO film with a low residual resistivity of ${\ensuremath{\rho}}_{0}\ensuremath{\approx}59\phantom{\rule{0.16em}{0ex}}\ensuremath{\mu}\mathrm{\ensuremath{\Omega}}\phantom{\rule{0.16em}{0ex}}\mathrm{cm}$, we found a zero-field dip in the magnetoresistance (MR) below 10 K, manifesting the ...
Tópico(s): Advanced Condensed Matter Physics
2017 - American Physical Society | Physical review. B./Physical review. B
Toshiki Nagakubo, Takuto Kumano, Takehiro Ohta, Yoshiteru Hashimoto, Michihiko Kobayashi,
Abstract Although cyclic imines are present in various bioactive secondary metabolites, their degradative metabolism remains unknown. Here, we report that copper amine oxidases, which are important in metabolism of primary amines, catalyze a cyclic imine cleavage reaction. We isolate a microorganism ( Arthrobacter sp. C-4A) which metabolizes a β-carboline alkaloid, harmaline. The harmaline-metabolizing enzyme (HarA) purified from strain C-4A is found to be copper amine oxidase and catalyze a ring-opening ...
Tópico(s): Pharmacogenetics and Drug Metabolism
2019 - Nature Portfolio | Nature Communications
Yasuyoshi Kurokawa, Hiroshi Ohta, M. Okubo, Michihiko Takahashi,
A homogeneous transparent gel was formed when an organic solution of cellulose acetate was in contact with a metal alkoxide solution. The gel is insoluble in common solvents and electrolyte solutions. It seems that the reaction is due to coordination bonding between hydroxy groups on the pyranose ring and the metal. Enzyme could be immobilized in the gel fibres. The immobilized enzyme is stable for a long period and can be used in a packed column.
Tópico(s): Electrochemical sensors and biosensors
1994 - Elsevier BV | Carbohydrate Polymers
Shinsuke Ohta, Osamu Matsui, Michihiko Yatazawa,
A tobacco callus strain, OMT-53, was selected from many cultures as a desirable strain having high nicotine producing capacity. Several culture conditions were examined, aiming to get higher nicotine production with the callus strain, OMT-53. It was revealed that the nicotine production was remarkably enhanced when the callus tissues were cultured at a limited concentration of α-NAA in culture medium. The optimal concentrations of sucrose and nitrogen in the culture medium were 3% and 840mgN/L respectively. ...
Tópico(s): Plant tissue culture and regeneration
1978 - Oxford University Press | Agricultural and Biological Chemistry
Masaki Yagi, Mio Kabata, Tomoyo Ukai, Sho Ohta, Akito Tanaka, Yui Shimada, Michihiko Sugimoto, Kimi Araki, Keisuke Okita, Knut Woltjen, Konrad Hochedlinger, Takuya Yamamoto, Yasuhiro Yamada,
CpG islands (CGIs) including those at imprinting control regions (ICRs) are protected from de novo methylation in somatic cells. However, many cancers often exhibit CGI hypermethylation, implying that the machinery is impaired in cancer cells. Here, we conducted a comprehensive analysis of CGI methylation during somatic cell reprogramming. Although most CGIs remain hypomethylated, a small subset of CGIs, particularly at several ICRs, was often de novo methylated in reprogrammed pluripotent stem cells ( ...
Tópico(s): Renal and related cancers
2019 - Elsevier BV | Stem Cell Reports
Shinsuke Ohta, Yoshihiro Kojima, Michihiko Yatazawa,
Nicotine biosynthesis in tobacco callus tissues was investigated by comparing high nicotine producing strain OMT-53 with scarcely producing strain NT-5. NT-5 strain which had been subcultured for about 5 years with a medium containing 1.0 ppm 2, 4-D, produced very little amount of nicotine even after more than 47 subcultures on OT-23 medium containing 0.15ppm α-NAA. On the other hand, OMT-53 strain maintained a constant high level nicotine producing (ca. 2% or more on D. W. basis) capacity over 50 ...
Tópico(s): Polyamine Metabolism and Applications
1978 - Oxford University Press | Agricultural and Biological Chemistry
Kazunobu Konishi, Kyoko Ishida, Ken-Ichi Oinuma, Takehiro Ohta, Yoshiteru Hashimoto, Hiroki Higashibata, Teizo Kitagawa, Michihiko Kobayashi,
Aldoxime dehydratase (OxdA), which is a novel heme protein, catalyzes the dehydration of an aldoxime to a nitrile even in the presence of water in the reaction mixture. The combination of site-directed mutagenesis of OxdA (mutation of all conserved histidines in the aldoxime dehydratase superfamily), estimation of the heme contents and specific activities of the mutants, and CD and resonance Raman spectroscopic analyses led to the identification of the proximal and distal histidines in this unique ...
Tópico(s): Heme Oxygenase-1 and Carbon Monoxide
2004 - Elsevier BV | Journal of Biological Chemistry
Michihiko Yamanouchi, Yasufumi Araki, Takaki Sakai, Tetsuya Uemura, Hiromichi Ohta, Jun’ichi Ieda,
In a ferromagnetic Weyl metal SrRuO3, a large effective magnetic field Heff exerted on a magnetic domain wall (DW) by current has been reported. We show that the ratio of Heff to current density exhibits nonmonotonic temperature dependence and surpasses those of conventional spin-transfer torques and spin-orbit torques. This enhancement is described well by topological Hall torque (THT), which is exerted on a DW by Weyl electrons emerging around Weyl points when an electric field is applied across ...
Tópico(s): Topological Materials and Phenomena
2022 - American Association for the Advancement of Science | Science Advances
Takayoshi Katase, H. Takahashi, Tetsuya Tohei, Yuki Suzuki, Michihiko Yamanouchi, Yuichi Ikuhara, Ichiro Terasaki, Hiromichi Ohta,
The oxygen‐deficient Sr 4− x Er x Co 4 O 12− δ (SECO), one of the ordered perovskite oxides, is a room‐temperature (RT) ferrimagnetic semiconductor that arises from the A‐site‐ordered structure. Development of such a material can lead to the realization of spintronic heterojunction devices; however, to this point there have been difficulties in achieving the A‐site‐ordered structure in SECO thin films. Here, single‐crystalline film growth of SECO with perfectly aligned A‐site‐ordered structure on ( ...
Tópico(s): Physics of Superconductivity and Magnetism
2015 - Wiley | Advanced Electronic Materials
Kazunobu Konishi, Takehiro Ohta, Ken-Ichi Oinuma, Yoshiteru Hashimoto, Teizo Kitagawa, Michihiko Kobayashi,
Recently, we discovered an intriguing hemoprotein [aliphatic aldoxime dehydratase (OxdA)] that catalyzes the dehydration of aliphatic aldoximes [R–CH=N–OH] to the corresponding nitriles [R–C≡N] in the industrial Pseudomonas chlororaphis B23 strain. Unlike the utilization of H 2 O 2 or O 2 as a mediator of the catalysis by other heme-containing enzymes (e.g., P450), OxdA is notable for the direct binding of a substrate to the heme iron, experimental evidence of which was obtained here by means of resonance ...
Tópico(s): Heme Oxygenase-1 and Carbon Monoxide
2006 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Ken-Ichi Oinuma, Takehiro Ohta, Kazunobu Konishi, Yoshiteru Hashimoto, Hiroki Higashibata, Teizo Kitagawa, Michihiko Kobayashi,
Resonance Raman spectra have been measured to characterize the heme environment in aldoxime dehydratase (OxdA), a novel hemoprotein, which catalyzes the dehydration of aldoxime into nitrile. The spectra showed that the ferric heme in the enzyme is six‐coordinate low spin, whereas the ferrous heme is five‐coordinate high spin. We assign a prominent vibration that occurs at 226 cm −1 in the ferrous enzyme to the Fe‐proximal histidine stretching vibration. In the CO‐bound form of OxdA, the correlation ...
Tópico(s): Heme Oxygenase-1 and Carbon Monoxide
2004 - Wiley | FEBS Letters
Michihiko Yamanouchi, Tatsuro Oyamada, Hiromichi Ohta,
We investigate the current-induced effective magnetic field Heff in La0.67Sr0.33MnO3 (18)/LaAlO3(0,2,6)/SrTiO3 (LSMO/LAO/STO) structures by using the planar Hall effect (PHE), where numbers in parentheses give the nominal thickness of the given layer in unit cells. In all the structures, applying an in-plane current creates an in-plane Heff orthogonal to the current direction, and the direction of Heff for the LSMO/LAO(6)/STO structure is opposite to that for the LSMO/LAO(0,2)/STO structures. At low ...
Tópico(s): Advanced Condensed Matter Physics
2020 - American Institute of Physics | AIP Advances
Mamoru Kimura, Toshihiro Kusunoki, Michihiko OHTA,
Glulam specimens with a large section were tested to find the effect of the processing accuracy of joints on the strength of bonded joints experimentally, furthermore bending tests were carried out with the full scale glulam specimens in order to investigate the bending strength of bonded joints. As a result, the following conclusions were obtained. Bending strength of the bonded joint is substantially subject to the processing accuracy of the joint part, and full strength connection of glulam with ...
Tópico(s): Advanced Cellulose Research Studies
1996 - | Journal of Structural and Construction Engineering (Transactions of AIJ)
Shizuo HAYASHI, Keiichi KATORI, Michihiko OHTA, Hiroyoshi YOSHIDA, Yasuo Higashibata,
Recently there are many cases in Japan to build by RC precast structures. It is important to make clear the shear behavior of RC precast structures, especially that of member-to-member joints. The easiness of connecting member with member is also important. The authors designed a new joint method connecting shear wall with floor slab, which was used by mesh bars under inclined arrangement. On this study the authors performed experiments under shear force to investigate the seismic behavior of this ...
Tópico(s): Urban and spatial planning
1994 - | Journal of Structural and Construction Engineering (Transactions of AIJ)
Shinsuke Ohta, Michihiko Yatazawa,
The effect of light on nicotine production in cultured tobacco callus tissues was investigated. Illumination strikingly inhibited the nicotine production even though the growth of the tissues was slightly stimulated by the light. The inhibitory effect of light increased as the intensity and the length of the illumination increased. No decisive difference in nicotine production was observed between the effects of blue light and red light. The depression of nicotine production by light was restored ...
Tópico(s): Soybean genetics and cultivation
1978 - Oxford University Press | Agricultural and Biological Chemistry
Junpei Nomura, Hiroshi Hashimoto, Takehiro Ohta, Yoshiteru Hashimoto, Koichi Wada, Yoshinori Naruta, Ken-Ichi Oinuma, Michihiko Kobayashi,
Aldoxime dehydratase (OxdA), which is a unique heme protein, catalyzes the dehydration of an aldoxime to a nitrile even in the presence of water in the reaction mixture. Unlike the utilization of H 2 O 2 or O 2 as a mediator of catalysis by other heme-containing enzymes (e.g., P450), OxdA is notable for the direct binding of a substrate to the heme iron. Here, we determined the crystal structure of OxdA. We then constructed OxdA mutants in which each of the polar amino acids lying within ∼6 Å of the ...
Tópico(s): Hemoglobin structure and function
2013 - National Academy of Sciences | Proceedings of the National Academy of Sciences
H. Fuke, Daisuke Akita, Issei Iijima, Naoki Izutsu, Yuka Kato, J. Kawada, Y. Matsuzaka, Eiichi Mizuta, Michiyoshi Namiki, N. Nonaka, Shigeo Ohta, Y. Saito, M. Seo, Atsushi Takada, Kouichi Tamura, Michihiko Toriumi, Kazuhiko Yamada, T. Yamagami, T. Yoshida,
Abstract Since 1971, numerous balloons have been launched from the Japanese balloon base, the Sanriku Balloon Center (SBC). Through these years, balloon technologies have been developed continuously and many scientific achievements have resulted. Recently, however, because of the limited area of the launching pad of the SBC, we have been faced with the difficulty of safely launching large balloons. To solve this issue, we decided to move the balloon base from the SBC to the Taiki Aerospace Research ...
Tópico(s): Cryospheric studies and observations
2009 - Elsevier BV | Advances in Space Research
Ken-Ichi Oinuma, Hideyuki Kumita, Takehiro Ohta, Kazunobu Konishi, Yoshiteru Hashimoto, Hiroki Higashibata, Teizo Kitagawa, Yoshitsugu Shiro, Michihiko Kobayashi,
On stopped‐flow analysis of aliphatic aldoxime dehydratase (OxdA), a novel hemoprotein, a spectrum derived from a reaction intermediate was detected on mixing ferrous OxdA with butyraldoxime; it gradually changed into that of ferrous OxdA with an isosbestic point at 421 nm. The spectral change on the addition of butyraldoxime to the ferrous H320A mutant showed the formation of a substrate‐coordinated mutant, the absorption spectrum of which closely resembled that of the above intermediate. These ...
Tópico(s): Protein Interaction Studies and Fluorescence Analysis
2005 - Wiley | FEBS Letters
Shigeru Nishizawa, Tetsuo Yokoyama, Seiji Ohta, Hiroshi Ryu, Toshiaki Ninchoji, Ichiro Shimoyama, Seiji Yamamoto, Yasushi Sugiura, Kenichi Uemura, Michihiko Nozue,
The authors report a case of a cerebellopontine (CP) angle lipoma with a very unusual histological appearance. The 38-year-old male patient suffered vertigo, left tinnitus, and left hearing disturbance. Computed tomography and magnetic resonance imaging showed a nonenhanced low-density area and a high-intensity region in the left CP angle, respectively. The tumor, which was only partially removed because of its tight adhesion to the VIIIth nerve and brainstem, consisted of mature lipocytes and contained ...
Tópico(s): Tumors and Oncological Cases
1990 - Japan Neurological Society | Neurologia medico-chirurgica
Michihiko Yamanouchi, Tatsuro Oyamada, Koichi Sato, Hiromichi Ohta, Jun’ichi Ieda,
We investigated the coercive field H c for domain wall (DW) motion as a function of the current I in the ferromagnetic oxide SrRuO 3 , a model system with narrow DWs for fabricating high-density spintronics devices. The DW is moved by I in the direction of the current, and H c is modulated linearly in I. This linear relationship is consistent with an effective magnetic field H eff driving the DW. The direction of DW motion and the magnitude of H eff are well described by a model based on the field- ...
Tópico(s): Physics of Superconductivity and Magnetism
2019 - IEEE Magnetics Society | IEEE Transactions on Magnetics
T. Yamagami, Michiyoshi Namiki, Y. Matsuzaka, Michihiko Toriumi, Yoshiaki Uchida, Shuichi Hirayama, Tatsuro Komatsu, H. Honda, Naoki Izutsu, Y. Saito, Shigeo Ohta, H. Hirosawa, Takaaki Matsumoto,
Abstract The Sanriku Balloon Center (SBC), which belongs to the Institute of Space and Astronautical Science, was built in 1971. The launching field of SBC was 140 m in length and 20 m in width. In Japan, the balloon launching method that we have been using since 1971 is a kind of static launching method. In 1998, we extended the launching field 20 m in length and created a new launcher at this point. We have recently developed a “semi-dynamic” launching method. This newly developed launcher is different ...
Tópico(s): Underwater Vehicles and Communication Systems
2002 - Elsevier BV | Advances in Space Research
Michiyoshi Namiki, Y. Matsuzaka, Hideyuki Honda, Michihiko Toriumi, Eiji Kamioka, Y. Saito, Naoki Izutsu, S. Ohta, T. Yamagami, Nobuyuki Yajima, H. Hirosawa, Nobuhiko Ohya, Fukashi Takezawa, Kenji Yamaguchi,
We describe a new Low Altitude Space Communication System (LASCOS), which was completed in 1996 by the Institute of Space and Astronautical Science in Japan. This system consists of a mobile balloon tracking and receiving station and networks which connect them to the Sanriku Balloon Center in Iwate Municipality and the Institute of Space and Astronautical Science (ISAS) in Kanagawa Municipality. This station and the SBC receiving station are connected via telephone lines, i. e. an Integrated Services ...
Tópico(s): Opportunistic and Delay-Tolerant Networks
2000 - Elsevier BV | Advances in Space Research
Shinsuke Ohta, Osamu Matsui, Michihiko Yatazawa,
... Tobacco Tissue Culture Get access Shinsuke Ohta, Shinsuke Ohta Department of Agricultural Chemistry, Faculty of Agriculture, Nagoya University, Nagoya, Japan Search for other works by this author on: Oxford Academic Google Scholar Osamu Matsui, Osamu Matsui Department of Agricultural Chemistry, Faculty of Agriculture, Nagoya University, Nagoya, Japan Search for other works by this author on: Oxford Academic Google Scholar Michihiko Yatazawa Michihiko Yatazawa Department of Agricultural Chemistry, Faculty ...
Tópico(s): Plant Virus Research Studies
1978 - Oxford University Press | Agricultural and Biological Chemistry